CN107312827A - A kind of SSR molecular marker method for identifying non-Bt cotton variety authentication - Google Patents
A kind of SSR molecular marker method for identifying non-Bt cotton variety authentication Download PDFInfo
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Abstract
The invention belongs to biological technical field, a kind of SSR molecular marker method for identifying non-Bt cotton variety authentication is disclosed, including：The preparation of sample；Using genomic DNA as template, enter performing PCR with 26 pairs of SSR core primers and expand, PCR primer is subjected to 8% native polyacrylamide gel electrophoresis, the electrophoresis pattern of detected sample is obtained；The electrophoresis pattern of standard sample by the electrophoresis pattern of every part of detected sample with obtaining is compared, and such as detected sample is consistent with the bands of a spectrum composition or banding pattern of standard sample, then seed to be detected and standard seed are same kind, draw the authenticity of seed to be detected.Result of the present invention is reliable and stable, simple to operate, economical and efficient, compared to Morphological Identification method have the advantages that efficiently and accurately, it is time saving and energy saving, be not subject to seasonal restrictions, preferable application prospect is illustrated, the identification and evaluation of non-Bt cotton variety authentication in production practices is preferably served.
Description
Technical field
The invention belongs to biological technical field, more particularly to a kind of SSR molecule marks for identifying non-Bt cotton variety authentication
Note method.
Background technology
Accurately, the quick identification for carrying out variety of crops, is distinguished for Crop breed audit, kind protection, true and false kind
Not, played an important role in terms of the property right dispute of kind.For a long time, both at home and abroad to cotton variety authenticity identification be all with
Based on morphology, seed morphology identification, seedling identification and field plot field plot test are specifically included.Seed morphology is identified
Be according to different cotton variety fibre lengths and regularity, short flannel it is how many and whether there is, the seed morphology such as the shape of cottonseed and color
Feature, and then judge variety authentication；Seedling identification method is mainly measured to some quantitative characters, according to the leaf color of cotton seedling,
The features such as blade profile, vein and blade face fine hair determine this kind and hybrid strain seedling number；Field plot field plot test method identification of species is true
Property when, identification should be observed to unginned cotton, gined cotton etc. after seedling stage, flower bud phase, flowering and boll-setting period, the term of opening bolls and harvest, foundation cotton
Strain, leaf, flower, the feature of bell and fiber quality traits evaluated and judged.
In summary, the problem of prior art is present be：
Seed morphology identification method is easily influenceed by a variety of production cultivation conditions, also extremely limited for the character identified,
When breed difference is small and during big quantity, differentiates just highly difficult.The method is above restricted in application, can only be referred to as one kind reference
Mark；Seedling identification method has been expanded using scope, but the method is mainly measured to some quantitative characters, is only used for identification product
The authenticity planted, it is impossible to be used in the special-shaped strain of identification；During the field plot test method identification of species authenticity of field plot, exist cycle length,
Time-consuming, investigation character is by the defect such as cultivation condition and such environmental effects, the artificial error of observation be larger.And testing result
Relatively lag behind, its ageing and reliability is also by a definite limitation, it is difficult to which the seed quality problem of monitoring in the market is conciliate in time
The certainly dispute such as kind infringement.
The content of the invention
The problem of existing for prior art, the invention provides a kind of the SSR for identifying non-Bt cotton variety authentication points
Sub- labeling method.
The present invention is achieved in that a kind of SSR molecular marker method for identifying non-Bt cotton variety authentication, the mirror
The SSR molecular marker method of permanent rule cotton variety authenticity, comprises the following steps：
The preparation of sample：3 sample progress are made in 15 individual plants of every part of sample detection, every 5 individual plants DNA mixed in equal amounts
Ssr analysis；
Using the genomic DNA of extraction as template, enter performing PCR with 26 pairs of SSR core primers and expand, resulting PCR is expanded
Increase production thing and carry out 8% native polyacrylamide gel electrophoresis, obtain the electrophoresis pattern of detected sample；
The electrophoresis pattern of standard sample by the electrophoresis pattern of every part of detected sample with obtaining is compared, such as to be detected
Sample is consistent with the bands of a spectrum composition or banding pattern of standard sample, then seed to be detected and standard seed are same kind, are drawn to be checked
Survey the authenticity of seed.
For 3 groups of inconsistent sample mixings of bands of a spectrum on same site, further analysis constitutes 15 individual plant collection of illustrative plates of the sample mixing,
Determine non-this product material；
The result judgement of cotton variety authenticity identification：It is basic using 26 pairs when carrying out cotton variety authenticity identification
Core mark is analyzed it, according to the similarities and differences of measuring samples and standard control sample sample mixing electrophoretic band, is judged by sample
The authenticity of product.
Further, using the quick grind away of CTAB method combination Automatic Grinding Prototypes of improvement, the genome of each individual plant is extracted
DNA。
Further, SSR core primers enter performing PCR amplification and included：
Select 10 μ L reaction volume；Including：
The 60ng μ L-1 μ L of DNA profiling 1,
4pmol μ L-1 each 0.4 μ L of forward and reverse primer,
The μ L of 10 × Buffer 1,
The 2.5mmol μ L of L-dNTP 0.8,
The 5U μ L-1 6.3 μ L of μ L and ddH2O of Taq enzyme 0.1, are expanded in PCR amplification instrument.
Further, the response procedures of PCR amplifications include：
95 DEG C of pre-degeneration 4min, 1 circulation；94 DEG C of denaturation 45s, 55 DEG C of annealing 35s, 72 DEG C of extension 45s, totally 35 are followed
Ring；72 DEG C of extension 7min, 4 DEG C of preservations.
Further, pcr amplification product detection includes：
SSR amplified productions use 8% native polyacrylamide gel electrophoresis to be detected, the SSR pieces of electrophoretic separation
Section is dyed with argentation.
Further, the electrophoresis pattern of standard sample of the electrophoresis pattern by every part of detected sample with obtaining is compared
Compared with specifically including：
When carrying out cotton variety authenticity identification, the overall banding pattern amplified in different cultivars using each pair primer is singly
Position carries out data record；The most generally and it is generally fixed combined type banding pattern, i.e. these banding patterns with 3 kinds~6 kinds banding patterns of presence
Several bands always exist simultaneously to disappear simultaneously, the overall band amplified according to each pair primer in kind to be measured and standard control
Type, compares standard control and the overall banding pattern difference of kind to be measured.
Further, the result of cotton variety authenticity identification, is specifically included：
When carrying out cotton variety authenticity identification using 26 core marks, when between standard sample and measuring samples collection of illustrative plates
When having 3 or more than 3 difference marks, interpretation is different cultivars；
When there is less than or equal to 2 difference marks between two kind sample mixing collection of illustrative plates, interpretation is approximate kind or same breed.
Further, 26 pairs of SSR core primers by with the larger material of 8 parts of hereditary differences to fixed on chromosome
586 pairs of polymorphism primers of position carry out preliminary screening, have extensive representational germ plasm resource secondary screening followed by 120 parts, most
26 pairs of polymorphism height, banding patterns clearly core primers are determined eventually；26 pairs of core primers are uniformly distributed on 26 chromosomes of cotton,
Detect 138 allelic variations altogether on 120 parts of secondary screening materials, average each SSR there are 5.31, and luffing is 2~9, its
In there is the site of polymorphism to be 129, polymorphism ratio is up to 93.48%；26 pairs of core primers generally distinguishing ability is still
Can, it can be widely applied in cotton variety identification.
Advantages of the present invention and good effect are：
A kind of method for identification non-Bt cotton variety authentication that the present invention is provided.It is demonstrated experimentally that result is reliable and stable, behaviour
Make simple, economical and efficient, compared to Morphological Identification method have the advantages that efficiently and accurately, it is time saving and energy saving, be not subject to seasonal restrictions, show
Preferable application prospect, preferably serves the identification and evaluation of non-Bt cotton variety authentication in production practices.Therefore, originally
Before invention SSR cores mark and non-Bt cotton Varieties identification method will have wide application in cotton variety identification field
Scape, to having reference function in other respective crop cultivar identification researchs.
Brief description of the drawings
Fig. 1 is the SSR molecular marker method flow diagram of identification non-Bt cotton variety authentication provided in an embodiment of the present invention.
Fig. 2 is primer NAU2083 provided in an embodiment of the present invention in standard sample and 3 groups of sample mixings of 10 parts of test samples
In AFLP system.
Fig. 3 is primer NAU2083 provided in an embodiment of the present invention to composition B1, B2, B3 and F1, F2, F3 15 individual plants
Collection of illustrative plates.
Embodiment
In order to make the purpose , technical scheme and advantage of the present invention be clearer, with reference to embodiments, to the present invention
It is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, it is not used to
Limit the present invention.
The application principle of the present invention is described in detail below in conjunction with the accompanying drawings
In the SSR molecular marker method of identification non-Bt cotton variety authentication provided in an embodiment of the present invention, core primers
Screening and checking：
The present invention using 8 parts of hereditary differences it is larger, with extensive representational cotton variety as sample material, to cotton point
The 586 pairs of primers announced in sub- registration database carry out preliminary screening, and the primer that primary dcreening operation is obtained is entered with 120 parts of germ plasm resources
Row checking, with reference to the distribution situation of SSR primers on chromosome, finally establishes 26 pairs and is evenly distributed on cotton chromosome
Primer is the preferred core primers (subordinate list 1) that cotton variety is identified.
1 26 pairs of SSR core primers information of table
| Numbering | Primer | Chromosome position | fwd_Sequence5'_3' | rev_Sequence5'_3' |
| 1 | NAU2083 | ch01 | AGAAGAGGTTGACGGTGAAG | TGAGTGAAGAACCTGCACAT |
| 2 | NAU2277 | ch02 | GAACTAGCCACATGATGCAC | TTGTTGAGGCATTAGTTTGC |
| 3 | NAU1071 | ch03 | ACCAACAATGGTGACCTCTT | CCCTCCATAACCAAAAGTTG |
| 4 | BNL530 | ch04 | CGTAGGATGGAAACGAAAGC | GCCACACTTTTCCCTCTCAA |
| 5 | NAU1200 | ch05 | CAACAGCAACAACCACAA | CTGCCTCGAGGACAAATAGT |
| 6 | CGR5651 | ch06 | TTTGGCTTAGCATTTGGAGG | CCGATCACTGTCCGTCTCTT |
| 7 | BNL1694 | ch07 | CGTTTGTTTTCGTGTAACAGG | TGGTGGATTCACATCCAAAG |
| 8 | DPL0111 | ch08 | CTTTCATAATACATACGCCTTGCC | TCACAGCATCCTATCAGGTATCAG |
| 9 | CGR5707 | ch09 | AAACCCGATATCCTTAGCCTTT | GGAAAGGAGGAAGAGGAGGA |
| 10 | NAU879 | ch10 | AGGAACCGATTCAAAGCTAA | TTTCCCCATTCTTGGTTAAG |
| 11 | BNL3442 | ch11 | CATTAGCGGATTTGTCGTGA | AACGAACAAAGCAAAGCGAT |
| 12 | BNL598 | ch12 | TATCTCCTTCACGATTCCATCAT | AAAAGAAAACAGGGTCAAAAGAA |
| 13 | CGR5576 | ch13 | CGGTTCAACCCGACTGTTT | GAGGAAAGAAAGGAAGAGAGGG |
| 14 | CIR246 | ch14 | TTAGGGTTTAGTTGAATGG | ATGAACACACGCACG |
| 15 | NAU3736 | ch15 | CATGTGCATTTCATCCTGTC | CCAAGTGAGAGGCATTTTCT |
| 16 | MUSS95 | ch16 | GCAACCATTAATTAAGCAAGTAACAA | CGAAGAATATGTGAACCTACAGAAAC |
| 17 | HAU1413 | ch17 | CTGACTTGGACCGAGAACTT | AACCAGGACCGATGAAATAA |
| 18 | TMB2295 | ch18 | TGAGTTCATGTTCCCCACTG | CTAAACATACTCTGTCAAACAC |
| 19 | BNL3977 | ch19 | ATCCAAACCAACCATGCAAT | GAAGGGGTTTTGCATTTCAA |
| 20 | JESPR190 | ch20 | GCCCGCCATCTTTGAGGATCCG | GGCAAAACTTGACAATTTTCTCGGC |
| 21 | JESPR158 | ch21 | CACCATTCGGCAGCTATTTC | CTGCAAACCCTAGCCTAGACG |
| 22 | NAU2026 | ch22 | GAATCTCGAAAACCCCATCT | ATTTGGAAGCGAAGTACCAG |
| 23 | JESPR13 | ch23 | GCTCTCAAATTGGCCTGTGT | GGTGGAGGCATTCCTGCTAAC |
| 24 | BNL3452 | ch24 | TGTAACTGAGCAGCCGTACG | GCCAAAGCAGAGTGAGATCC |
| 25 | BNL3937 | ch25 | ACATCAAACAAAGCAAGCCA | ATCTCTGTTTTCTCCCCCGT |
| 26 | NAU1042 | ch26 | CATGCAAATCCATGCTAGAG | GGTTTCTTTGGTGGTGAAAC |
Wherein, the sequence of NAU2083 fwd_Sequence5'_3' core primers is designated as：SED ID NO：1.
As shown in figure 1, the SSR molecular marker method of identification non-Bt cotton variety authentication provided in an embodiment of the present invention,
Comprise the following steps：
S101:The preparation of sample：3 samples are made in 15 individual plants of every part of sample detection, every 5 individual plants DNA mixed in equal amounts
(A1, A2, A3) is analyzed；
S102:Using the genomic DNA of extraction as template, enter performing PCR with 26 pairs of SSR core primers and expand, will be resulting
Pcr amplification product carries out 8% native polyacrylamide gel electrophoresis, obtains the electrophoresis pattern of detected sample；
S103:The standard sample that the electrophoresis pattern of every part of detected sample and the method according to the S101 and S102 are obtained
The electrophoresis pattern of product (X1, X2, X3) is compared, and such as the bands of a spectrum of detected sample and standard sample constitute (banding pattern) unanimously, then
The seed to be detected is same kind with standard seed, draws the authenticity of seed to be detected.
S104:For 3 groups of inconsistent sample mixings of bands of a spectrum on same site, further analysis constitutes 15 lists of the sample mixing
Strain collection of illustrative plates, determines non-this product material.
S105:The result judgement of cotton variety authenticity identification：When carrying out cotton variety authenticity identification, 26 pairs are utilized
Taproot mark is analyzed it, according to the similarities and differences of measuring samples and standard control sample sample mixing electrophoretic band, judge by
The authenticity of sample product.
If adding up in 26 SSR markers：
A) by sample product and standard control differences between samples reference numerals ＞ 2, interpretation is different cultivars；
B) by sample product and standard control differences between samples reference numerals≤2, interpretation is approximate kind；
C) by sample product and standard control differences between samples reference numerals=0, interpretation is identical or extremely approximate kind
According to above-mentioned authenticity identification scheme, 10 parts of Upland Cottons (strain) that upper main cultivation is produced to Xinjiang are reflected
Fixed, its qualification result and field test results are completely the same.It is demonstrated experimentally that SSR-DNA fingerprint techniques of the present invention can quickly, it is accurate
True identification cotton variety authenticity.
The application principle of the present invention is further described with reference to specific embodiment.
SSR cores mark the step of expanding and detect into performing PCR as follows：
1.1 preparation of samples
Upland Cotton of the experiment from the upper main cultivation of production：Including source cotton No. 6, new land is early No. 13, new land is early No. 26, new
No. 37 in early No. 37 of land, No.9 Xinluzhong, new land, Shandong cotton grind No. 28, CCRI 35, CCRI 41, CCRI No. 43 10
Kind is authenticity identification material, and each kind randomly selects 15 individual plants, and the leaf DNA mixed in equal amounts of every 5 individual plants is carried out
Ssr analysis.
1.2 DNA extraction
Using the quick grind away of CTAB method combination Automatic Grinding Prototypes of improvement, the genomic DNA of each individual plant is extracted.Due to
Purity requirement of the SSR marker to template DNA be not high, so performing PCR amplification can directly be entered by being not required to purifying DNA.
1.3 SSR-PCR are expanded
1.3.1 reaction system
10 μ L reaction volume：The μ L of DNA profiling (60ng μ L-1) 1, forward and reverse primer (4pmol μ L-1) each 0.4 μ L, 10
The 0.8 μ L of μ L, dNTP (2.5mmol L-1) of × Buffer 1, the 6.3 μ L of μ L and ddH2O of Taq enzyme (5U μ L-1) 0.1, expand in PCR
Increase and expanded on instrument.
1.3.2 response procedures
95 DEG C of pre-degeneration 4min, 1 circulation；94 DEG C of denaturation 45s, 55 DEG C of annealing 35s, 72 DEG C of extension 45s, totally 35 are followed
Ring；72 DEG C of extension 7min, 4 DEG C of preservations.Because each pair SSR primers have its specific annealing temperature, therefore in amplification program
Annealing temperature is slightly changed according to the actual Tm values of different primers, and other programs are constant.
1.4 PCR primers are detected
SSR amplified productions use 8% native polyacrylamide gel electrophoresis to be detected, the SSR pieces of electrophoretic separation
Section is dyed with argentation.
The interpretation of 1.5 SSR AFLP systems
When carrying out cotton variety authenticity identification, the overall banding pattern amplified in different cultivars using each pair primer is singly
Position carries out data record.The most universal and be generally fixed combined type banding pattern there is 3-6 kinds banding pattern, i.e., these banding patterns is several
Band always exists simultaneously to disappear simultaneously, the overall band that can be amplified according to each pair primer in kind to be measured and standard control
Type, compares standard control and the overall banding pattern difference of kind to be measured, effective to simplify compared with common " 0 ", " 1 " note band method
Workload, while human error can be avoided.
1.6 cotton variety authenticities are identified
It is object to choose 10 cotton conventional varieties, and it is numbered (table 2), randomly selects No. 6 product of 15 plants of source cottons
Kind, 1 group is uniformly mixed into 5 individual plant DNA, 3 samples (A1, A2, A3) are made as standard control.By measuring samples
3 samples (X1, X2, X3) are made according to the method described above, standard sample and measuring samples are expanded using 26 pairs of core marks
Increase, by analyzing the difference site of the overall collection of illustrative plates of 10 measuring samples and standard control, judge the authenticity of measuring samples.
Table 2 is used for 10 non-Bt cotton kinds of authenticity identification
Table2 List of 10conventional cotton varieties for authenticity
identification
1.6.1 difference Locus Analysis in Shoots between 3 groups of sample mixings of same sample
Found by 3 groups of sample mixing electrophoresis patterns for contrasting same sample, wherein 3 groups of source cotton No. 6 and CCRI 43 are mixed
Bands of a spectrum are consistent during sample is marked at 26；There is the inconsistent (figure of 4-13 site bands of a spectrum between 3 groups of sample mixings of remaining 8 kind
2).It has been observed that in the inconsistent site of bands of a spectrum, 3 groups of sample mixings of each kind show two kinds of amplification banding patterns.For same
3 groups of inconsistent sample mixings of bands of a spectrum on site, further analysis constitutes 15 individual plant collection of illustrative plates (Fig. 3) of the sample mixing, determines non-this product
Material.
1.6.2 difference Locus Analysis in Shoots between test sample and standard sample
Define and be limited with 3 difference marks according to the different cotton varieties of experiment setting：Cotton is carried out using 26 core marks
During flower variety authenticity identification, when having 3 or more than 3 difference marks between standard sample and measuring samples collection of illustrative plates, it can sentence
Read as different cultivars；When there is less than or equal to 2 differences mark between two kind sample mixing collection of illustrative plates, can interpretation be approximate kind or phase
Same kind.In 10 test samples, only to expand on 26 sites banding pattern with standard sample completely the same for A1-A3 samples, its
There is 8-18 difference site in remaining sample, in fact A1-A3 samples are 3 points of samples of No. 6 kinds of source cotton with standard sample,
Its qualification result and field test results are completely the same.
The identification of test sample and standard sample is not only realized using the present invention, and 10 also being capable of phase by sample product
Mutually distinguish.In the lab using 26 pairs of core primers to being detected by sample product authenticity, its detection is only needed 1-2 days
Complete, and result is quick, accurate
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention
Any modifications, equivalent substitutions and improvements made within refreshing and principle etc., should be included in the scope of the protection.
Claims (8)
1. a kind of SSR molecular marker method for identifying non-Bt cotton variety authentication, it is characterised in that the identification non-Bt cotton
The SSR molecular marker method of variety authentication, comprises the following steps：
The preparation of sample：15 individual plants of every part of sample detection, every 5 individual plants DNA mixed in equal amounts is made 3 samples and carries out SSR points
Analysis；
Using the genomic DNA of extraction as template, enter performing PCR with 26 pairs of SSR core primers and expand, resulting PCR is expanded and produced
Thing carries out 8% native polyacrylamide gel electrophoresis, obtains the electrophoresis pattern of detected sample；
The electrophoresis pattern of standard sample by the electrophoresis pattern of every part of detected sample with obtaining is compared, such as detected sample
Consistent with the bands of a spectrum composition or banding pattern of standard sample, then seed to be detected and standard seed are same kind, draw kind to be detected
The authenticity of son；
For 3 groups of inconsistent sample mixings of bands of a spectrum on same site, further analysis constitutes 15 individual plant collection of illustrative plates of the sample mixing, it is determined that
Non- this product material；
The result judgement of cotton variety authenticity identification：When carrying out cotton variety authenticity identification, 26 pairs of taproots are utilized
Mark is analyzed it, according to the similarities and differences of measuring samples and standard control sample sample mixing electrophoretic band, is judged by sample product
Authenticity.
2. the SSR molecular marker method of non-Bt cotton variety authentication is identified as claimed in claim 1, it is characterised in that adopted
With the quick grind away of CTAB method combination Automatic Grinding Prototypes of improvement, the genomic DNA of each individual plant is extracted.
3. the SSR molecular marker method of non-Bt cotton variety authentication is identified as claimed in claim 1, it is characterised in that SSR
Core primers, which enter performing PCR amplification, to be included：
Select 10 μ L reaction volume；Including：
The 60ng μ L-1 μ L of DNA profiling 1,
4pmol μ L-1 each 0.4 μ L of forward and reverse primer,
The μ L of 10 × Buffer 1,
The 2.5mmol μ L of L-dNTP 0.8,
The 5U μ L-1 6.3 μ L of μ L and ddH2O of Taq enzyme 0.1, are expanded in PCR amplification instrument.
4. the SSR molecular marker method of non-Bt cotton variety authentication is identified as claimed in claim 1, it is characterised in that PCR
The response procedures of amplification include：
95 DEG C of pre-degeneration 4min, 1 circulation；94 DEG C of denaturation 45s, 55 DEG C of annealing 35s, 72 DEG C of extension 45s, totally 35 circulations；72
DEG C extension 7min, 4 DEG C preservation.
5. the SSR molecular marker method of non-Bt cotton variety authentication is identified as claimed in claim 1, it is characterised in that PCR
Amplified production detection includes：
SSR amplified productions are detected that the SSR fragments of electrophoretic separation are used using 8% native polyacrylamide gel electrophoresis
Argentation is dyed.
6. the SSR molecular marker method of non-Bt cotton variety authentication is identified as claimed in claim 1, it is characterised in that institute
The electrophoresis pattern for stating the standard sample by the electrophoresis pattern of every part of detected sample with obtaining is compared, and is specifically included：
When carrying out cotton variety authenticity identification, entered in units of the overall banding pattern that each pair primer is amplified in different cultivars
Row data record；The most generally and to be generally fixed combined type banding pattern in the presence of 3 kinds~6 kinds banding patterns, i.e., these banding patterns is several
Band always exists simultaneously to disappear simultaneously, the overall banding pattern amplified according to each pair primer in kind to be measured and standard control,
Compare standard control and the overall banding pattern difference of kind to be measured.
7. the SSR molecular marker method of non-Bt cotton variety authentication is identified as claimed in claim 1, it is characterised in that cotton
The result of flower variety authenticity identification, is specifically included：
When carrying out cotton variety authenticity identification using 26 cores mark, when having 3 between standard sample and measuring samples collection of illustrative plates
During individual or more than 3 difference marks, interpretation is different cultivars；
When there is less than or equal to 2 difference marks between two kind sample mixing collection of illustrative plates, interpretation is approximate kind or same breed.
8. the SSR molecular marker method of non-Bt cotton variety authentication is identified as claimed in claim 1, it is characterised in that institute
26 pairs of SSR core primers are stated by drawing with the larger material of 8 parts of hereditary differences to oriented 586 pairs of polymorphisms on chromosome
Thing carries out preliminary screening, has extensive representational germ plasm resource secondary screening followed by 120 parts, finally determines 26 pairs of polymorphisms
High, banding pattern clearly core primers；26 pairs of core primers are uniformly distributed on 26 chromosomes of cotton, in 120 parts of secondary screening materials
On detect 138 allelic variations altogether, average each SSR has 5.31, and luffing is 2~9, wherein the position with polymorphism
Point is 129.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110527743A (en) * | 2019-09-20 | 2019-12-03 | 石河子大学 | For identifying polymorphic molecular marker and the application of Xinjiang colour cotton variety |
| CN111663002A (en) * | 2020-07-14 | 2020-09-15 | 福建农林大学 | Microsatellite molecular marker for distinguishing genetic background of second chromosome of high-noble variety and dense variety of cutting hand of sugarcane and application of microsatellite molecular marker |
| CN114410815A (en) * | 2021-12-31 | 2022-04-29 | 石河子大学 | Method for constructing Xinjiang upland cotton variety fingerprint spectrum |
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| CN111663002A (en) * | 2020-07-14 | 2020-09-15 | 福建农林大学 | Microsatellite molecular marker for distinguishing genetic background of second chromosome of high-noble variety and dense variety of cutting hand of sugarcane and application of microsatellite molecular marker |
| CN114410815A (en) * | 2021-12-31 | 2022-04-29 | 石河子大学 | Method for constructing Xinjiang upland cotton variety fingerprint spectrum |
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