CN107311733A - A kind of production method of high content adenosine fruiting bodies of cordyceps militaris - Google Patents
A kind of production method of high content adenosine fruiting bodies of cordyceps militaris Download PDFInfo
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- CN107311733A CN107311733A CN201710318519.8A CN201710318519A CN107311733A CN 107311733 A CN107311733 A CN 107311733A CN 201710318519 A CN201710318519 A CN 201710318519A CN 107311733 A CN107311733 A CN 107311733A
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- cordyceps militaris
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D9/00—Other inorganic fertilisers
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
Abstract
The invention discloses a kind of production method of high content adenosine fruiting bodies of cordyceps militaris, fruiting bodies of cordyceps militaris is cultivated in the medium, component includes the 120g of peanut 80 that shells by weight in 2000g culture mediums, the 120g of raw soya bean 80, the 550g of rice 450, the 60g of tomato 40, the dry 60g of dried silkworm chrysalis meal 40, the 60g of fish meal 40, the fresh 60g of beef 40, the 60g of potato 40, the 20g of glucose 10, the 10g of dusty yeast 5, the 20g of sucrose 10, the 30g of agar 20, the 60mg of vitamin B1 20, straw leachate 450ml 550ml and soil extract 450ml 550ml, preparation technology of the present invention is simple, obtained fruiting bodies of cordyceps militaris adenosine recovery rate is high.
Description
Technical field
The present invention relates to fruiting bodies of cordyceps militaris production technical field, specially a kind of high content adenosine fruiting bodies of cordyceps militaris
Production method.
Background technology
Cordyceps militaris also known as northern Chinese caterpillar Fungus, Cordceps militaris etc., xenogenesis is belonged to together with cordyceps sinensis, is the type sepecies of Cordyceps, category
It is that it colonizes in the stroma and ossified pupa grown on Insecta Lepidoptera noctuid pupal cell in Ascomycotina Cordyceps
The complex of body.Years of researches show that there are Cordyceps militaris a variety of pharmacology such as immunological regulation, antitumor, antiviral, anti-infective to live
Property, is widely used in tumour, immunologic hypofunction including HIV infection etc. in a variety of diseases.
Adenosine is a kind of endogenous purine nucleoside acid, with extensive physiologically active, the content of adenosine and winter in Cordyceps militaris
Worm summer grass is similar, even more high, more more than 0.01%.So far separating identification from cordyceps sinensis and fermented hypha has 11 nucleosides
Class compound, including adenine, adenosine, uracil, uridine, guanine, guanosine, hypoxanthine, inosine, thymus gland are phonetic
Pyridine, thymidine and uracil deoxyribonucleoside etc., fruiting bodies of cordyceps militaris extraction adenosine efficiency is low in the prior art, carry
Take rate low, therefore, existing processing method how is improved in technology path and processing technology, it has been urgently to solve to improve adenosine content
Certainly, and significant problem.
The content of the invention
It is an object of the invention to provide a kind of production method of high content adenosine fruiting bodies of cordyceps militaris, to solve the above-mentioned back of the body
The problem of being proposed in scape technology.
To achieve the above object, the present invention provides following technical scheme：A kind of high content adenosine fruiting bodies of cordyceps militaris, it is described
Fruiting bodies of cordyceps militaris is cultivated in the medium, and component includes shell peanut 80-120g, life by weight in 2000g culture mediums
Soya bean 80-120g, rice 450-550g, tomato 40-60g, dry dried silkworm chrysalis meal 40-60g, fish meal 40-60g, fresh beef 40-60g,
Potato 40-60g, glucose 10-20g, dusty yeast 5-10g, sucrose 10-20g, agar 20-30g, vitamin B1 20-60mg, rice
Careless leachate 450ml-550ml and soil extract 450ml-550ml.
It is preferred that, the preferred component proportioning of culture medium is：Shell peanut 100g, raw soya bean 100g, rice 500g, tomato
50g, dry dried silkworm chrysalis meal 50g, fish meal 50g, fresh beef 50g, potato 50g, glucose 15g, dusty yeast 8g, sucrose 15g, agar 2g,
Vitamin B1 40mg, straw leachate 500ml and soil extract 500ml.
It is preferred that, the straw leaches liquid and preparation method thereof and comprised the following steps：
A, take straw 45-55g, add in pulverizer and crush, pulverizer rotating speed is 2000-3000 revs/min, grinding time is
10min-20min, obtains straw end；
600-800g water is added in B, the straw end for obtaining step A, water is boiled into 50min-70min, mixed liquor is obtained, afterwards
It is cooled to normal temperature；
C, the mixed liquor for obtaining step B are added and centrifuged in centrifuge, that is, obtain straw leachate.
It is preferred that, the soil extract preparation method comprises the following steps：
A, the soil 80-120g for taking water saturation, add water 500-600ml afterwards；
B, soil and water is sufficiently stirred for 10-20min；
Supernatant, as soil extract are taken after C, afterwards standing 24h.
It is preferred that, the fruiting bodies of cordyceps militaris preparation method comprises the following steps：
A, the peanut that will shell, raw soya bean, tomato, dry dried silkworm chrysalis meal, fish meal, fresh beef, potato, glucose, dusty yeast, sucrose,
Add in pulverizer and crush after agar, vitamin B1 mixing, pulverizer rotating speed is 3000-4000 revs/min, and grinding time is
20min-40min, obtains mixture A；
B, add rice, straw leachate, soil extract in mixture A and poured into after being sufficiently mixed in Culture basin, it
After carry out high-temperature sterilization；
C, normal temperature is cooled to afterwards, strain is accessed in an aseptic environment；
D, step C culture medium cultivated 6-8 days under no light conditions, until media surface grows mycelia；
E, the culture medium for afterwards obtaining step D are cultivated 3 days in the environment of suitable light, 20 DEG C of temperature, complete annesl, induce button
Grow；
F, culture medium cultivated 6 weeks in the environment of middle light, 22 DEG C of temperature afterwards, that is, obtain fruiting bodies of cordyceps militaris.
Compared with prior art, the beneficial effects of the invention are as follows：Preparation technology of the present invention is simple, and obtained Cordyceps militaris is real
Body adenosine recovery rate is high；The present invention extracts Cordyceps militaris adenosine content height, and the content of adenosine can reach every gram of raw material extraction and obtain
2.95mg amount, the recovery rate of final active component may be up to 0.23%, all higher than other general extraction methods recovery rates, and
Quality controllable, material damages few, with short production cycle, is adapted to large-scale production, and market potential is huge.
Embodiment
The technical scheme in the embodiment of the present invention is clearly and completely described below, it is clear that described embodiment
Only a part of embodiment of the invention, rather than whole embodiments.Based on the embodiment in the present invention, the common skill in this area
The every other embodiment that art personnel are obtained under the premise of creative work is not made, belongs to the model that the present invention is protected
Enclose.
Invention provides following technical scheme：
Embodiment one：
A kind of high content adenosine fruiting bodies of cordyceps militaris, the fruiting bodies of cordyceps militaris is cultivated in the medium, group in 2000g culture mediums
Part by weight include shell peanut 80g, raw soya bean 80g, rice 450g, tomato 40g, dry dried silkworm chrysalis meal 40g, fish meal 40g,
Fresh beef 40g, potato 40g, glucose 10g, dusty yeast 5g, sucrose 10g, agar 20g, vitamin B1 20mg, straw leachate
450ml and soil extract 450ml.
In the present embodiment, straw leaches liquid and preparation method thereof and comprised the following steps：
A, take straw 45g, add in pulverizer and crush, pulverizer rotating speed is 2000 revs/min, and grinding time is 10min, obtains rice
Careless end；
600g water is added in B, the straw end for obtaining step A, water is boiled into 50min, mixed liquor is obtained, is cooled to afterwards often
Temperature；
C, the mixed liquor for obtaining step B are added and centrifuged in centrifuge, that is, obtain straw leachate.
In the present embodiment, soil extract preparation method comprises the following steps：
A, the soil 80g for taking water saturation, add water 500ml afterwards；
B, soil and water is sufficiently stirred for 10min；
Supernatant, as soil extract are taken after C, afterwards standing 24h.
In the present embodiment, fruiting bodies of cordyceps militaris preparation method comprises the following steps：
A, the peanut that will shell, raw soya bean, tomato, dry dried silkworm chrysalis meal, fish meal, fresh beef, potato, glucose, dusty yeast, sucrose,
Add in pulverizer and crush after agar, vitamin B1 mixing, pulverizer rotating speed is 3000 revs/min, and grinding time is 20min, is obtained
To mixture A；
B, add rice, straw leachate, soil extract in mixture A and poured into after being sufficiently mixed in Culture basin, it
After carry out high-temperature sterilization；
C, normal temperature is cooled to afterwards, strain is accessed in an aseptic environment；
D, step C culture medium cultivated 6 days under no light conditions, until media surface grows mycelia；
E, the culture medium for afterwards obtaining step D are in suitable light（450lx）, cultivate 3 days in the environment of 20 DEG C of temperature, complete annesl,
Induction button is grown；
F, afterwards by culture medium in middle light（600lx）, cultivate 6 weeks in the environment of 22 DEG C of temperature, that is, obtain fruiting bodies of cordyceps militaris.
Embodiment two：
A kind of high content adenosine fruiting bodies of cordyceps militaris, the fruiting bodies of cordyceps militaris is cultivated in the medium, group in 2000g culture mediums
Part includes shell peanut 120g, raw soya bean 120g, rice 550g, tomato 60g, dry dried silkworm chrysalis meal 60g, fish meal by weight
60g, fresh beef 60g, potato 60g, glucose 20g, dusty yeast 10g, sucrose 20g, agar 30g, vitamin B1 60mg, straw
Leachate 550ml and soil extract 550ml.
In the present embodiment, straw leaches liquid and preparation method thereof and comprised the following steps：
A, take straw 55g, add in pulverizer and crush, pulverizer rotating speed is 3000 revs/min, and grinding time is 20min, obtains rice
Careless end；
800g water is added in B, the straw end for obtaining step A, water is boiled into 70min, mixed liquor is obtained, is cooled to afterwards often
Temperature；
C, the mixed liquor for obtaining step B are added and centrifuged in centrifuge, that is, obtain straw leachate.
In the present embodiment, soil extract preparation method comprises the following steps：
A, the soil 120g for taking water saturation, add water 600ml afterwards；
B, soil and water is sufficiently stirred for 20min；
Supernatant, as soil extract are taken after C, afterwards standing 24h.
In the present embodiment, fruiting bodies of cordyceps militaris preparation method comprises the following steps：
A, the peanut that will shell, raw soya bean, tomato, dry dried silkworm chrysalis meal, fish meal, fresh beef, potato, glucose, dusty yeast, sucrose,
Add in pulverizer and crush after agar, vitamin B1 mixing, pulverizer rotating speed is 4000 revs/min, and grinding time is 40min, is obtained
To mixture A；
B, add rice, straw leachate, soil extract in mixture A and poured into after being sufficiently mixed in Culture basin, it
After carry out high-temperature sterilization；
C, normal temperature is cooled to afterwards, strain is accessed in an aseptic environment；
D, step C culture medium cultivated 8 days under no light conditions, until media surface grows mycelia；
E, the culture medium for afterwards obtaining step D are cultivated 3 days in the environment of suitable light, 20 DEG C of temperature, complete annesl, induce button
Grow；
F, culture medium cultivated 6 weeks in the environment of middle light, 22 DEG C of temperature afterwards, that is, obtain fruiting bodies of cordyceps militaris.
Embodiment three：
A kind of high content adenosine fruiting bodies of cordyceps militaris, the fruiting bodies of cordyceps militaris is cultivated in the medium, group in 2000g culture mediums
Part by weight include shell peanut 90g, raw soya bean 90g, rice 480g, tomato 45g, dry dried silkworm chrysalis meal 45g, fish meal 45g,
Fresh beef 45g, potato 45g, glucose 12g, dusty yeast 6g, sucrose 12g, agar 22g, vitamin B1 30mg, straw leachate
480ml and soil extract 480ml.
In the present embodiment, straw leaches liquid and preparation method thereof and comprised the following steps：
A, take straw 48g, add in pulverizer and crush, pulverizer rotating speed is 2200 revs/min, and grinding time is 12min, obtains rice
Careless end；
650g water is added in B, the straw end for obtaining step A, water is boiled into 55min, mixed liquor is obtained, is cooled to afterwards often
Temperature；
C, the mixed liquor for obtaining step B are added and centrifuged in centrifuge, that is, obtain straw leachate.
In the present embodiment, soil extract preparation method comprises the following steps：
A, the soil 90g for taking water saturation, add water 520ml afterwards；
B, soil and water is sufficiently stirred for 12min；
Supernatant, as soil extract are taken after C, afterwards standing 24h.
In the present embodiment, fruiting bodies of cordyceps militaris preparation method comprises the following steps：
A, the peanut that will shell, raw soya bean, tomato, dry dried silkworm chrysalis meal, fish meal, fresh beef, potato, glucose, dusty yeast, sucrose,
Add in pulverizer and crush after agar, vitamin B1 mixing, pulverizer rotating speed is 3200 revs/min, and grinding time is 25min, is obtained
To mixture A；
B, add rice, straw leachate, soil extract in mixture A and poured into after being sufficiently mixed in Culture basin, it
After carry out high-temperature sterilization；
C, normal temperature is cooled to afterwards, strain is accessed in an aseptic environment；
D, step C culture medium cultivated 6.5 days under no light conditions, until media surface grows mycelia；
E, the culture medium for afterwards obtaining step D are cultivated 3 days in the environment of suitable light, 20 DEG C of temperature, complete annesl, induce button
Grow；
F, culture medium cultivated 6 weeks in the environment of middle light, 22 DEG C of temperature afterwards, that is, obtain fruiting bodies of cordyceps militaris.
Example IV：
A kind of high content adenosine fruiting bodies of cordyceps militaris, the fruiting bodies of cordyceps militaris is cultivated in the medium, group in 2000g culture mediums
Part includes shell peanut 110g, raw soya bean 110g, rice 520g, tomato 55g, dry dried silkworm chrysalis meal 55g, fish meal by weight
55g, fresh beef 55g, potato 55g, glucose 18g, dusty yeast 9g, sucrose 18g, agar 28g, vitamin B1 50mg, straw leaching
Go out liquid 520ml and soil extract 520ml.
In the present embodiment, straw leaches liquid and preparation method thereof and comprised the following steps：
A, take straw 52g, add in pulverizer and crush, pulverizer rotating speed is 2800 revs/min, and grinding time is 18min, obtains rice
Careless end；
750g water is added in B, the straw end for obtaining step A, water is boiled into 65min, mixed liquor is obtained, is cooled to afterwards often
Temperature；
C, the mixed liquor for obtaining step B are added and centrifuged in centrifuge, that is, obtain straw leachate.
In the present embodiment, soil extract preparation method comprises the following steps：
A, the soil 110g for taking water saturation, add water 550ml afterwards；
B, soil and water is sufficiently stirred for 18min；
Supernatant, as soil extract are taken after C, afterwards standing 24h.
In the present embodiment, fruiting bodies of cordyceps militaris preparation method comprises the following steps：
A, the peanut that will shell, raw soya bean, tomato, dry dried silkworm chrysalis meal, fish meal, fresh beef, potato, glucose, dusty yeast, sucrose,
Add in pulverizer and crush after agar, vitamin B1 mixing, pulverizer rotating speed is 3800 revs/min, and grinding time is 35min, is obtained
To mixture A；
B, add rice, straw leachate, soil extract in mixture A and poured into after being sufficiently mixed in Culture basin, it
After carry out high-temperature sterilization；
C, normal temperature is cooled to afterwards, strain is accessed in an aseptic environment；
D, step C culture medium cultivated 7.5 days under no light conditions, until media surface grows mycelia；
E, the culture medium for afterwards obtaining step D are cultivated 3 days in the environment of suitable light, 20 DEG C of temperature, complete annesl, induce button
Grow；
F, culture medium cultivated 6 weeks in the environment of middle light, 22 DEG C of temperature afterwards, that is, obtain fruiting bodies of cordyceps militaris.
Embodiment five：
A kind of high content adenosine fruiting bodies of cordyceps militaris, the fruiting bodies of cordyceps militaris is cultivated in the medium, group in 2000g culture mediums
Part includes shell peanut 100g, raw soya bean 100g, rice 500g, tomato 50g, dry dried silkworm chrysalis meal 50g, fish meal by weight
50g, fresh beef 50g, potato 50g, glucose 15g, dusty yeast 8g, sucrose 15g, agar 2g, vitamin B1 40mg, straw leaching
Go out liquid 500ml and soil extract 500ml.
In the present embodiment, straw leaches liquid and preparation method thereof and comprised the following steps：
A, take straw 50g, add in pulverizer and crush, pulverizer rotating speed is 2500 revs/min, and grinding time is 15min, obtains rice
Careless end；
700g water is added in B, the straw end for obtaining step A, water is boiled into 60min, mixed liquor is obtained, is cooled to afterwards often
Temperature；
C, the mixed liquor for obtaining step B are added and centrifuged in centrifuge, that is, obtain straw leachate.
In the present embodiment, soil extract preparation method comprises the following steps：
A, the soil 100g for taking water saturation, add water 550ml afterwards；
B, soil and water is sufficiently stirred for 15min；
Supernatant, as soil extract are taken after C, afterwards standing 24h.
In the present embodiment, fruiting bodies of cordyceps militaris preparation method comprises the following steps：
A, the peanut that will shell, raw soya bean, tomato, dry dried silkworm chrysalis meal, fish meal, fresh beef, potato, glucose, dusty yeast, sucrose,
Add in pulverizer and crush after agar, vitamin B1 mixing, pulverizer rotating speed is 3500 revs/min, and grinding time is 30min, is obtained
To mixture A；
B, add rice, straw leachate, soil extract in mixture A and poured into after being sufficiently mixed in Culture basin, it
After carry out high-temperature sterilization；
C, normal temperature is cooled to afterwards, strain is accessed in an aseptic environment；
D, step C culture medium cultivated 7 days under no light conditions, until media surface grows mycelia；
E, the culture medium for afterwards obtaining step D are cultivated 3 days in the environment of suitable light, 20 DEG C of temperature, complete annesl, induce button
Grow；
F, culture medium cultivated 6 weeks in the environment of middle light, 22 DEG C of temperature afterwards, that is, obtain fruiting bodies of cordyceps militaris.
Preparation technology of the present invention is simple, and obtained fruiting bodies of cordyceps militaris adenosine recovery rate is high；The present invention extracts Cordyceps militaris gland
Glycosides content is high, and the content of adenosine can reach every gram of raw material and extract the amount for obtaining 2.95mg, and the recovery rate of final active component can
Up to 0.23%, all higher and quality controllable than other general extraction methods recovery rates, material damages few, with short production cycle, is adapted to big
Large-scale production, market potential is huge.
Although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
A variety of changes, modification can be carried out to these embodiments, replace without departing from the principles and spirit of the present invention by understanding
And modification, the scope of the present invention is defined by the appended.
Claims (5)
1. a kind of high content adenosine fruiting bodies of cordyceps militaris, it is characterised in that：The fruiting bodies of cordyceps militaris is cultivated in the medium,
Component includes shell peanut 80-120g, raw soya bean 80-120g, rice 450-550g, west by weight in 2000g culture mediums
Red persimmon 40-60g, dry dried silkworm chrysalis meal 40-60g, fish meal 40-60g, fresh beef 40-60g, potato 40-60g, glucose 10-20g, ferment
Female powder 5-10g, sucrose 10-20g, agar 20-30g, vitamin B1 20-60mg, straw leachate 450ml-550ml and soil
Earth leachate 450ml-550ml.
2. a kind of high content adenosine fruiting bodies of cordyceps militaris according to claim 1, it is characterised in that：The preferred group of culture medium
Part matches：Shell peanut 100g, raw soya bean 100g, rice 500g, tomato 50g, dry dried silkworm chrysalis meal 50g, fish meal 50g, fresh ox
Meat 50g, potato 50g, glucose 15g, dusty yeast 8g, sucrose 15g, agar 2g, vitamin B1 40mg, straw leachate 500ml
And soil extract 500ml.
3. realize a kind of production method of high content adenosine fruiting bodies of cordyceps militaris described in claim 1, it is characterised in that：It is described
Straw leaches liquid and preparation method thereof and comprised the following steps：
A, take straw 45-55g, add in pulverizer and crush, pulverizer rotating speed is 2000-3000 revs/min, grinding time is
10min-20min, obtains straw end；
600-800g water is added in B, the straw end for obtaining step A, water is boiled into 50min-70min, mixed liquor is obtained, afterwards
It is cooled to normal temperature；
C, the mixed liquor for obtaining step B are added and centrifuged in centrifuge, that is, obtain straw leachate.
4. realize a kind of production method of high content adenosine fruiting bodies of cordyceps militaris described in claim 1, it is characterised in that：It is described
Soil extract preparation method comprises the following steps：
The soil 80-120g of water saturation is taken, water 500-600ml is added afterwards；
Soil and water are sufficiently stirred for 10-20min；
Supernatant, as soil extract are taken after standing 24h afterwards.
5. realize a kind of production method of high content adenosine fruiting bodies of cordyceps militaris described in claim 1, it is characterised in that：It is described
Fruiting bodies of cordyceps militaris preparation method comprises the following steps：
A, the peanut that will shell, raw soya bean, tomato, dry dried silkworm chrysalis meal, fish meal, fresh beef, potato, glucose, dusty yeast, sucrose,
Add in pulverizer and crush after agar, vitamin B1 mixing, pulverizer rotating speed is 3000-4000 revs/min, and grinding time is
20min-40min, obtains mixture A；
B, add rice, straw leachate, soil extract in mixture A and poured into after being sufficiently mixed in Culture basin, it
After carry out high-temperature sterilization；
C, normal temperature is cooled to afterwards, strain is accessed in an aseptic environment；
D, step C culture medium cultivated 6-8 days under no light conditions, until media surface grows mycelia；
E, the culture medium for afterwards obtaining step D are cultivated 3 days in the environment of suitable light, 20 DEG C of temperature, complete annesl, induce button
Grow；
F, culture medium cultivated 6 weeks in the environment of middle light, 22 DEG C of temperature afterwards, that is, obtain fruiting bodies of cordyceps militaris.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110002920A (en) * | 2019-05-06 | 2019-07-12 | 信阳农林学院 | A kind of purple leaf barberry dedicated fertilizer and preparation method thereof |
| KR20210027875A (en) * | 2019-09-03 | 2021-03-11 | 경상국립대학교산학협력단 | Culture method for enhancing cordycepin content of Paecilomyces javanicus Mycelium using Light-Emitting Diode |
| KR20210027873A (en) * | 2019-09-03 | 2021-03-11 | 경상국립대학교산학협력단 | Culture method for enhancing content of Paecilomyces javanicus mycelium using Light-Emitting Diode |
| KR20210027876A (en) * | 2019-09-03 | 2021-03-11 | 경상국립대학교산학협력단 | Culture method for enhancing content of Cordyceps militaris mycelium using Light-Emitting Diode |
| KR20210027874A (en) * | 2019-09-03 | 2021-03-11 | 경상국립대학교산학협력단 | Culture method for enhancing cordycepin content of Cordyceps Militaris Mycelium using Light-Emitting Diode |
| CN112568056A (en) * | 2020-12-08 | 2021-03-30 | 锦州聚红农业科技有限公司 | High-adenosine cordyceps militaris cultivation method |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH09327232A (en) * | 1996-06-11 | 1997-12-22 | Oubiken:Kk | Medium for cultivation of 'tochu-kasou' |
| CN104086305A (en) * | 2014-06-30 | 2014-10-08 | 桂林丰茂源农业技术开发有限公司 | Culture medium for cordyceps militaris fruiting body |
-
2017
- 2017-05-08 CN CN201710318519.8A patent/CN107311733A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH09327232A (en) * | 1996-06-11 | 1997-12-22 | Oubiken:Kk | Medium for cultivation of 'tochu-kasou' |
| CN104086305A (en) * | 2014-06-30 | 2014-10-08 | 桂林丰茂源农业技术开发有限公司 | Culture medium for cordyceps militaris fruiting body |
Non-Patent Citations (2)
| Title |
|---|
| 张红 等: "不同培养基对蛹虫草子实体中虫草素和腺苷含量的影响", 《北京农业》 * |
| 黄盼宁: "以稻草为外加碳源的研究进展", 《科技创新与应用》 * |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110002920A (en) * | 2019-05-06 | 2019-07-12 | 信阳农林学院 | A kind of purple leaf barberry dedicated fertilizer and preparation method thereof |
| KR20210027875A (en) * | 2019-09-03 | 2021-03-11 | 경상국립대학교산학협력단 | Culture method for enhancing cordycepin content of Paecilomyces javanicus Mycelium using Light-Emitting Diode |
| KR20210027873A (en) * | 2019-09-03 | 2021-03-11 | 경상국립대학교산학협력단 | Culture method for enhancing content of Paecilomyces javanicus mycelium using Light-Emitting Diode |
| KR20210027876A (en) * | 2019-09-03 | 2021-03-11 | 경상국립대학교산학협력단 | Culture method for enhancing content of Cordyceps militaris mycelium using Light-Emitting Diode |
| KR20210027874A (en) * | 2019-09-03 | 2021-03-11 | 경상국립대학교산학협력단 | Culture method for enhancing cordycepin content of Cordyceps Militaris Mycelium using Light-Emitting Diode |
| KR102356056B1 (en) | 2019-09-03 | 2022-01-26 | 경상국립대학교산학협력단 | Culture method for enhancing content of Paecilomyces javanicus mycelium using Light-Emitting Diode |
| KR102356058B1 (en) * | 2019-09-03 | 2022-01-26 | 경상국립대학교산학협력단 | Culture method for enhancing cordycepin content of Paecilomyces javanicus Mycelium using Light-Emitting Diode |
| KR102356057B1 (en) * | 2019-09-03 | 2022-01-26 | 경상국립대학교산학협력단 | Culture method for enhancing cordycepin content of Cordyceps Militaris Mycelium using Light-Emitting Diode |
| KR102356059B1 (en) | 2019-09-03 | 2022-01-26 | 경상국립대학교산학협력단 | Culture method for enhancing content of Cordyceps militaris mycelium using Light-Emitting Diode |
| CN112568056A (en) * | 2020-12-08 | 2021-03-30 | 锦州聚红农业科技有限公司 | High-adenosine cordyceps militaris cultivation method |
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