CN107308495A - A kind of artificial blood vessel based on trees-Osima jacoti, Osima excavata and preparation method thereof - Google Patents
A kind of artificial blood vessel based on trees-Osima jacoti, Osima excavata and preparation method thereof Download PDFInfo
- Publication number
- CN107308495A CN107308495A CN201710546641.0A CN201710546641A CN107308495A CN 107308495 A CN107308495 A CN 107308495A CN 201710546641 A CN201710546641 A CN 201710546641A CN 107308495 A CN107308495 A CN 107308495A
- Authority
- CN
- China
- Prior art keywords
- blood vessel
- artificial blood
- osima
- trees
- preparation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/28—Materials for coating prostheses
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/18—Macromolecular materials obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/507—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials for artificial blood vessels
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/54—Biologically active materials, e.g. therapeutic substances
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/42—Anti-thrombotic agents, anticoagulants, anti-platelet agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2430/00—Materials or treatment for tissue regeneration
- A61L2430/40—Preparation and treatment of biological tissue for implantation, e.g. decellularisation, cross-linking
Abstract
The invention discloses a kind of artificial blood vessel based on trees-Osima jacoti, Osima excavata and preparation method and application, the intestinal mucosa lower floor after crushing is added to the water, then enzyme is inactivated after pH is 7.5 8.5 times addition trypsase, enzymolysis, obtains degradation solution；Degradation solution is coated in artificial blood vessel's inwall, the artificial blood vessel based on trees-Osima jacoti, Osima excavata is dried to obtain.The invention by SIS degradation solutions be used for modified biological artificial blood vessel, build a kind of with antithrombotic biological artificial blood vessel, existing small-caliber artificial blood vessel easily thrombosed problem is efficiently solved, while preparation process advantages of simple, beneficial to clinical practice.
Description
Technical field
The present invention relates to a kind of medical material, and in particular to a kind of artificial blood vessel and its system based on trees-Osima jacoti, Osima excavata
Preparation Method.
Background technology
When the blood vessel of human body a part is because the reasons such as aging, artery sclerosis, embolism or breakage are it cannot be guaranteed that human normal
During blood supply, the surgical operations such as line replacement, bridging or intervention need to be entered using artificial blood vessel and are treated.Preferable artificial blood vessel application
Good histocompatbility, antithrombotic, physical stability, anti-infection property and usability, and excellent mechanical property and good
Good blood tissues compatibility, then be the key of artificial blood vessel technological break-through.
Numerous studies show that intact animal small intestinal mucosa epithelial cell shows to contain a large amount of mucins in slime layer, except
It is outer from physically or chemically damaging with protective epithelium cell, the function of the occurrence and development of prevention of various diseases is also participated in, such as
Prevent cystic fibrosis disease, myxoadenocarcinoma, inflammatory enteritis etc..Chitterlings are mainly constituted by 4 layers, from inside to outside respectively mucous membrane
Layer, submucosa, muscle layer and placenta percreta.Trees-Osima jacoti, Osima excavata is made up of one layer of fine and close connective tissue, main component be I,
Type III collagenous fibres, contain the larger blood vessel of a small amount of cell and part, lymphatic vessel and nerve.
Recent research shows that SIS materials have better effects for the reparation of alimentary canal defect；But, simple SIS materials
Material is repaired after alimentary canal, the narrow of anastomotic position easily occurs.Trace it to its cause for the material be it is a kind of it is acellular, rich in collagen, and
A kind of biomaterial containing the tactophily factor.After simple SIS materials implant, it is very easy to receive host cell,
And response to traume caused by implant surgery, fibroblast hyperproliferative is often led to, finally forms narrow with scar repairing.
At present, medium-large caliber vasotransplantation achieves preferable clinical effectiveness, at a specified future date unobstructed ideal, but small-bore
(interior diameter is less than 6 mm), artificial blood vessel easily formed thrombus or intimal hyperplasia because its VPV is slow, the low feature of blood pressure,
So as to cause long-term patency rate after transplanting low.Wherein, host blood vessel and artificial blood vessel's compliance are mismatched, and can cause the blood of blood
Hydromechanics state change, so as to cause thrombosis, causes the graft failure of artificial pipe.Currently it is directed to small-caliber artificial blood vessel
The research of compliance is concentrated mainly in terms of vascular graftses, structure and post-processing approach.
The content of the invention
It is empty with pig it is an object of the invention to provide a kind of artificial blood vessel based on trees-Osima jacoti, Osima excavata and preparation method thereof
Intestines are raw material, and the intestinal mucosa lower floor of natural extracellular matrix class bio-derived material is prepared using Mechanical Method（SIS）, application
SDS, Tris, EDTA are further handled SIS prepared by Mechanical Method, are removed residual cell, then with trypsin hydrolysis, are obtained
Attachment proteins, the novel vascular obtained from is good biomaterial, excellent with good biocompatibility, nonhazardous effect etc.
Point, will not cause Human immune responses, and good biocompatibility is shown in clinical experiment.
The invention discloses a kind of preparation method of the artificial blood vessel based on trees-Osima jacoti, Osima excavata, comprise the following steps,
Intestinal mucosa lower floor after artificial blood vessel's inwall coats enzymolysis, is dried to obtain the artificial blood based on trees-Osima jacoti, Osima excavata
Pipe.
In above-mentioned technical proposal, the artificial blood vessel includes artificial terylene blood vessel, silk artificial blood vessel, expanded PTFE second
Alkene artificial blood vessel, polyurethane artificial blood vessel；It is preferred that the artificial blood vessel is polyurethane artificial blood vessel, its preparation method is to gather
Extruded, solidified after urethane liquation vacuum defoamation, drawn and obtain polyurethane fiber, then by polyurethane fiber wound on circle
On mould, then through supersolidification pre-setting handle, be dried to obtain polyurethane artificial blood vessel.
In above-mentioned technical proposal, intestinal mucosa lower floor is digested with trypsase.
In above-mentioned technical proposal, the intestinal mucosa lower floor after crushing is added to the water, then added under pH is 7.5-8.5
Enzyme is inactivated after entering trypsase, enzymolysis, degradation solution is obtained；Degradation solution is coated in artificial blood vessel's inwall, is dried to obtain based on pig
The artificial blood vessel of submucous layer of small intestine.
In above-mentioned technical proposal, regulation pH is PBS solution for 7.5-8.5 reagent；Enzymolysis time is 5-24 hours, enzymolysis
Temperature is 37-50 DEG C；Enzyme is inactivated using boiling；Using the mode of injection degradation solution is coated in artificial blood vessel's inwall；Drying is
Drying at room temperature 1 hour so that the SIS hydrolysis after crushing is abundant, and its activity can be kept by being coated in artificial blood vessel.
In above-mentioned technical proposal, the mass ratio of intestinal mucosa lower floor and water is 1-10: 99-90；The consumption of trypsase
For the 30-60% of intestinal mucosa lower floor quality so that intestinal mucosa lower floor can be fully hydrolyzed.
In above-mentioned technical proposal, intestinal mucosa lower floor is prepared as, and is handled using Mechanical Method after pig jejunum, recycles ten
Sodium dialkyl sulfate, three (methylol) aminomethanes, ethylenediamine tetra-acetic acid, finally remove residual cell, obtain intestinal mucosa
Lower floor.
Such as specific steps：Fresh chitterlings are cleaned up, placenta percreta and muscle layer are removed by the method for mechanical curettage,
Then by remaining submucous layer of small intestine in containing 0.1% Peracetic acid, the solution of 10% ethanol immersion treatment 1h, use deionization
2h is soaked after water cleaning in methanol, chloroform mixed liquor, then successively using hypertonic solution immersion 2h, detergent solution immersion 2h,
Hypotonic solution soaks 2h, detergent solution immersion 2h.Obtained de- cell trees-Osima jacoti, Osima excavata is put into 1mol/L NaOH molten
1min is soaked in liquid；With being freeze-dried after deionized water rinsing；Each material composition of described hypertonic solution is：The high vadose solutions of 1L
Liquid sodium chloride containing 58.44g, 6.06gTris, 2.92gEDTA, 1L purified water；Each material composition of hypotonic solution：1L is hypotonic molten
Liquid purified water containing 1.21gTris, 1.46gEDTA, 1L；Each material composition of detergent solution：1L detergent solutions contain 15g ten
Dialkyl sulfonates, 1L purified waters.By resulting lyophilized SDS, low temperature breaks into powder, adds trypsin hydrolysis, prepares viscous
Attached albumen, is coated on artificial blood vessel as coating
The invention discloses the artificial blood vessel based on trees-Osima jacoti, Osima excavata prepared according to above-mentioned preparation method.
The invention discloses application of the trees-Osima jacoti, Osima excavata in artificial blood vessel is prepared.
The invention discloses application of the trees-Osima jacoti, Osima excavata in artificial blood vessel's inside coating is prepared.
The invention discloses a kind of preparation method of artificial blood vessel's inside coating, comprise the following steps, by the pig after enzymolysis
Small intestinal submucosa is coated on artificial blood vessel's inwall, is dried to obtain artificial blood vessel's inside coating.
Artificial blood vessel based on trees-Osima jacoti, Osima excavata prepared by the present invention is good biologic bracket material, blood vessel
Contain a variety of growth factors such as basic fibroblast growth factor（bFGF）, TGF β（TGF-β）, blood vessel endothelium
Porcine HGF (VEGF) and sulfated proteoglycans, fibronectin（fibronectin, FN）Deng containing liver simultaneously
Element, Heparan sulfate, hyaluronic acid etc..Liquaemin is also known as heparin, is that a kind of acid mucopolysaccharides containing sulfate is natural
Anticoagulative substance, is combined into complex with protein and exists.Heparin has strong negative electrical charge, hydrophily and more activity in itself
Functional group, still, constituting the polymer of artificial blood vessel has very strong chemical inertness and hydrophobicity, it is impossible to direct with heparin molecule
React, although there are a variety of methods pre-processed to artificial blood vessel at present, there is required instrument and equipment costliness, to material sheet
The problems such as body result influences again, operating procedure is complicated, is not only difficult to widely use but also causes heparin stably can not be present in blood vessel
Inwall.Artificial blood vessel disclosed by the invention contains heparin simultaneously in addition to containing a variety of growth factors, Heparan sulfate, thoroughly
Bright matter acid etc., and resistance to blood washes away, so as to excellent antithrombotic property, especially thoroughly solve small-bore (interior straight
Footpath is less than 6 mm) artificial blood vessel can thrombosed problem.
Existing small-caliber artificial blood vessel easily forms thrombus, and new intima, which is thickened, is easily caused obliteration.The present invention is artificial
The inwall of blood vessel is uniformly coated with SIS degradation solution, and artificial blood vessel endothelialization effect is good, and the barrier action of vascular endothelial cell can be very
Strengthen the anti-bolt ability of grafting vessel in big degree, can significantly improve the usual rate of small-caliber artificial blood vessel.Due to degraded
Liquid has adhesiveness, can be very good to stick to the mucin in blood vessel, and degradation solution except with protective epithelium cell
It is outer from physically or chemically damaging, also participate in the function of the occurrence and development of prevention of various diseases；Due to containing heparin in inwall
Sodium so that the artificial blood vessel of preparation has antithrombotic,；Due to containing a variety of growth factors in inwall, blood is overcome to move in body
Washing away and pressure for mechanics, actively captures the endothelial progenitor cells in circulation, and promotes to nourish angeogenesis, promotes blood vessel to plant
Enter thing to mould in body weight, so as to effectively play the effect of antithrombus formation and endometrial hyperplasia, improve small-bore biological artificial blood vessel and move
Patency rate after plant.
SIS degradation solutions are used for modified biological artificial blood vessel by the invention, and building a kind of has an antithrombus formation
Biological artificial blood vessel, efficiently solve existing small-caliber artificial blood vessel easily thrombosed problem, while preparation process is simple
Rationally, beneficial to clinical practice.
Embodiment
The present invention is further elaborated on below by embodiment, but skilled in the art realises that, reality of the invention
Apply example not limiting the scope of the invention, any improvement and change made on the basis of the present invention, all in the present invention
Protection domain within.
Embodiment one
1.0g lyophilized SIS samples are weighed, is clayed into power, is transferred in test tube with grater, the pure of magneton and 90mL is added
Change water, be uniformly mixed, add 500mg trypsase, under 50 degree, stirring reaction 5h after enzymolysis terminates, boils, made
Enzyme is inactivated, and produces degradation solution.
At room temperature, polyurethane particles are added in tetrahydrofuran, 2h is stirred with 100 turns/min speed；By polyurethane
Solution is placed in the environment that vacuum is 0.3Mpa and carries out deaeration；Place after 3h, take out polyurethane solutions and extruded, extruded
Polyurethane solutions pre-processed by coagulating bath, long thread polyurethane is then pulled out in draw-gear, then will long thread poly- ammonia
Ester is on the cylindrical die of rotation；The cylindrical die for being wound with long thread polyurethane is placed in absolute ethyl alcohol and soaked
Steep 36h and carry out the qualitative processing of coagulating bath, be prepared into hollow tubular thing, then remove hollow tubular thing from cylindrical die surface,
Control temperature that 36h is dried to hollow tubular thing for 30 degree, that is, obtain one-time formed polyurethane artificial blood vessel.
SIS degradation solutions are filled in 20mL syringe, syringe is connected with artificial blood vessel one end, SIS degradation solutions are pushed away
Injection artificial blood vessel's tube chamber simultaneously makes coating solution be spread evenly across lumen wall, and unnecessary SIS degradation solutions are from artificial blood vessel's other end
Remove.Artificial blood vessel after coating dries 1h at room temperature, produces the artificial blood vessel based on trees-Osima jacoti, Osima excavata.
Artificial blood vessel's prosthese thrombus generation based on intestinal mucosa lower floor significantly reduces to reduce artificial blood vessel plant
Enter the danger that rear embolism occurs, and make its softness is flexible to possess preferably handling.
Embodiment two
1.0g lyophilized SIS samples are weighed, is clayed into power, is transferred in test tube with grater, the pure of magneton and 95mL is added
Change water, be uniformly mixed, add 400mg trypsase, under 37 degree, stirring reaction 24h after enzymolysis terminates, boils, made
Enzyme is inactivated, and produces degradation solution.
At room temperature, polyurethane particles are added in tetrahydrofuran, 2h is stirred with 100 turns/min speed；By polyurethane
Solution is placed in the environment that vacuum is 0.3Mpa and carries out deaeration；Place after 3h, take out polyurethane solutions and extruded, extruded
Polyurethane solutions pre-processed by coagulating bath, long thread polyurethane is then pulled out in draw-gear, then will long thread poly- ammonia
Ester is on the cylindrical die of rotation；The cylindrical die for being wound with long thread polyurethane is placed in absolute ethyl alcohol and soaked
Steep 36h and carry out the qualitative processing of coagulating bath, be prepared into hollow tubular thing, then remove hollow tubular thing from cylindrical die surface,
Control temperature that 36h is dried to hollow tubular thing for 30 degree, that is, obtain one-time formed polyurethane artificial blood vessel.
SIS degradation solutions are filled in 20mL syringe, syringe is connected with artificial blood vessel one end, SIS degradation solutions are pushed away
Injection artificial blood vessel's tube chamber simultaneously makes coating solution be spread evenly across lumen wall, and unnecessary SIS degradation solutions are from artificial blood vessel's other end
Remove.Artificial blood vessel after coating dries 1h at room temperature, produces the artificial blood vessel based on trees-Osima jacoti, Osima excavata.
, can be by induction for modifying artificial blood vessel due to containing neurotrophic factor in intestinal mucosa lower floor
Skin progenitor cells actively capture to promote interior ginned cotton, play the effect of antithrombus formation and endometrial hyperplasia.
Embodiment three
1.0g lyophilized SIS samples are weighed, is clayed into power, is transferred in test tube with grater, the pure of magneton and 90mL is added
Change water, be uniformly mixed, add 600mg trypsase, under 40 degree, stirring reaction 12h after enzymolysis terminates, boils, made
Enzyme is inactivated, and produces degradation solution.
At room temperature, polyurethane particles are added in tetrahydrofuran, 2h is stirred with 100 turns/min speed；By polyurethane
Solution is placed in the environment that vacuum is 0.3Mpa and carries out deaeration；Place after 3h, take out polyurethane solutions and extruded, extruded
Polyurethane solutions pre-processed by coagulating bath, long thread polyurethane is then pulled out in draw-gear, then will long thread poly- ammonia
Ester is on the cylindrical die of rotation；The cylindrical die for being wound with long thread polyurethane is placed in absolute ethyl alcohol and soaked
Steep 36h and carry out the qualitative processing of coagulating bath, be prepared into hollow tubular thing, then remove hollow tubular thing from cylindrical die surface,
Control temperature that 36h is dried to hollow tubular thing for 30 degree, that is, obtain one-time formed polyurethane artificial blood vessel.
SIS degradation solutions are filled in 20mL syringe, syringe is connected with artificial blood vessel one end, SIS degradation solutions are pushed away
Injection artificial blood vessel's tube chamber simultaneously makes coating solution be spread evenly across lumen wall, and unnecessary SIS degradation solutions are from artificial blood vessel's other end
Remove.Artificial blood vessel after coating dries 1h at room temperature, produces the artificial blood vessel based on trees-Osima jacoti, Osima excavata.
Example IV
10.0g lyophilized SIS samples are weighed, is clayed into power, is transferred in test tube with grater, the pure of magneton and 99mL is added
Change water, be uniformly mixed, add 500mg trypsase, under 37 degree, stirring reaction 5h after enzymolysis terminates, boils, made
Enzyme is inactivated, and produces degradation solution.SIS degradation solutions are filled in 20mL syringe, syringe is connected with silk artificial blood vessel one end,
SIS degradation solutions are injected into artificial blood vessel's tube chamber and coating solution is spread evenly across lumen wall, and unnecessary SIS degradation solutions are from people
The work blood vessel other end is removed.Artificial blood vessel after coating dries 1h at room temperature, produces the artificial blood based on trees-Osima jacoti, Osima excavata
Pipe, plays the effect of antithrombus formation and endometrial hyperplasia.
Embodiment five
10.0g lyophilized SIS samples are weighed, is clayed into power, is transferred in test tube with grater, the pure of magneton and 90mL is added
Change water, be uniformly mixed, add 400mg trypsase, under 50 degree, stirring reaction 20h after enzymolysis terminates, boils, made
Enzyme is inactivated, and produces degradation solution.SIS degradation solutions are filled in 20mL syringe, syringe and the artificial blood of expanded ptfe
Pipe one end is connected, and SIS degradation solutions are injected into artificial blood vessel's tube chamber and coating solution is spread evenly across lumen wall, unnecessary
SIS degradation solutions are removed from artificial blood vessel's other end.Artificial blood vessel after coating dries 1h at room temperature, produces based on pig intestinal mucosa
The artificial blood vessel of lower floor, plays the effect of antithrombus formation and endometrial hyperplasia.
In the present invention, handled using Mechanical Method after pig jejunum, recycle lauryl sodium sulfate, three (methylol) amino first
Alkane, ethylenediamine tetra-acetic acid, finally remove residual cell, obtain intestinal mucosa lower floor（SIS）, it is with PBS solution regulation pH
7.5-8.5；The present invention is evenly coated in SIS degradation solution on artificial blood vessel's inwall, prepares the artificial blood with anti-thrombus function
Pipe.Reduce the permeability of blood；Endothelial growth factor can increase vasopermeability, promote vascular endothelial cell proliferation.Compared with
The effect of few thrombus, improves the compliance of artificial blood vessel, reaches the purpose matched with host blood vessel compliance.
It is can be seen that from embodiment one with prior art Contrast on effect, choose the artificial blood vessel of non-coating, measure average blood
Permeability is 23.2mL/min.cm2, and the blood penetration rate of the artificial blood vessel prepared according to the present invention is 0.012mL/
min.cm2, close to zero.
Thrombosis compares before and after artificial blood vessel prosthese coating（Experiment in vitro）, 0.25mL citron acidemia is mixed
0.1mmol/L CaCl2 25ul injects the artificial blood vessel prosthese lumen wall of above-mentioned preparation, the above-mentioned blood injection of same volume
Control is used as in the artificial blood vessel prosthese tube chamber of non-coating.5mL distilled water is poured over experiment sample at 5,10 and 15min respectively
It is that experiment is obtained that data in Hirschfeld-Klinger reaction, and the situation of the formation of each blood vessel prosthesis sample observation thrombus, table 1 below are terminated on this
Semi-quantitative parameters result；The artificial blood vessel of other embodiment also obtains the technique effect approximate with embodiment one.
The semi-quantitative parameters result of table 1
As shown in table 1, collagen coating group is formed in 5,10min time points without fibrin clot, in 15min time point collagen eggs
White coating group fibrin clot forms same considerably less than control group；The artificial blood vessel prosthese of non-coating when with contacting blood
It is almost hydrophobic, the fiber of woven artificial blood vessel need to be just soaked close to 20 seconds blood, and blood is soaked under pig intestinal mucosa
The artificial blood vessel matrix of layer is balanced and rapid.
Claims (10)
1. a kind of preparation method of the artificial blood vessel based on trees-Osima jacoti, Osima excavata, it is characterised in that comprise the following steps, in people
Intestinal mucosa lower floor after work blood vessel coating enzymolysis, is dried to obtain the artificial blood vessel based on trees-Osima jacoti, Osima excavata.
2. the preparation method of the artificial blood vessel based on trees-Osima jacoti, Osima excavata according to claim 1, it is characterised in that described
Artificial blood vessel includes artificial terylene blood vessel, silk artificial blood vessel, expanded ptfe artificial blood vessel, polyurethane artificial blood vessel.
3. the preparation method of the artificial blood vessel based on trees-Osima jacoti, Osima excavata according to claim 2, it is characterised in that described
The preparation method of polyurethane artificial blood vessel is that will be extruded, solidified after polyurethane liquation vacuum defoamation, drawn and obtain polyurethane
Fiber, then by polyurethane fiber on circular die, then through supersolidification pre-setting is handled, to be dried to obtain polyurethane artificial
Blood vessel.
4. the preparation method of the artificial blood vessel based on trees-Osima jacoti, Osima excavata according to claim 1, it is characterised in that use pancreas
Protease is digested to intestinal mucosa lower floor；The mass ratio of intestinal mucosa lower floor and water is（1~10）∶（99~90）；
The consumption of trypsase is the 30~60% of intestinal mucosa lower floor quality.
5. the preparation method of the artificial blood vessel based on trees-Osima jacoti, Osima excavata according to claim 1, it is characterised in that by powder
Intestinal mucosa lower floor after broken is added to the water, and then inactivates enzyme after pH is 7.5~8.5 times addition trypsase, enzymolysis,
Obtain degradation solution；Degradation solution is coated in artificial blood vessel's inwall, the artificial blood vessel based on trees-Osima jacoti, Osima excavata is dried to obtain.
6. the preparation method of the artificial blood vessel based on trees-Osima jacoti, Osima excavata according to claim 5, it is characterised in that regulation
PH is PBS for 7.5~8.5 reagent；Enzymolysis time is 5~24 hours, and hydrolysis temperature is 37~50 DEG C；Enzyme is lost using boiling
It is living；Using the mode of injection degradation solution is coated in artificial blood vessel's inwall；Dry and be drying at room temperature 1 hour.
7. the preparation method of the artificial blood vessel based on trees-Osima jacoti, Osima excavata according to claim 5, it is characterised in that pig is small
Intestinal mucosa lower floor is prepared as, and is handled using Mechanical Method after pig jejunum, recycles lauryl sodium sulfate, three (methylol) amino
Methane, ethylenediamine tetra-acetic acid, finally remove residual cell, obtain intestinal mucosa lower floor.
8. the artificial blood vessel based on trees-Osima jacoti, Osima excavata prepared by preparation method according to claim 1.
9. application of the trees-Osima jacoti, Osima excavata in artificial blood vessel or artificial blood vessel's inside coating is prepared.
10. a kind of preparation method of artificial blood vessel's inside coating, it is characterised in that comprise the following steps, small by the pig after enzymolysis
Intestinal mucosa lower floor is coated on artificial blood vessel's inwall, is dried to obtain artificial blood vessel's inside coating.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710546641.0A CN107308495A (en) | 2017-07-06 | 2017-07-06 | A kind of artificial blood vessel based on trees-Osima jacoti, Osima excavata and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710546641.0A CN107308495A (en) | 2017-07-06 | 2017-07-06 | A kind of artificial blood vessel based on trees-Osima jacoti, Osima excavata and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107308495A true CN107308495A (en) | 2017-11-03 |
Family
ID=60177555
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710546641.0A Withdrawn CN107308495A (en) | 2017-07-06 | 2017-07-06 | A kind of artificial blood vessel based on trees-Osima jacoti, Osima excavata and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107308495A (en) |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1559360A (en) * | 2004-02-19 | 2005-01-05 | 重庆大学 | Method for planting esoderma/endothelial cell on inner surface of artificial blood vessel |
| CN101002967A (en) * | 2006-12-27 | 2007-07-25 | 重庆大学 | Imaginal stem cell membrane stent in blood vessel, and its preparing method |
| CN104189009A (en) * | 2014-09-01 | 2014-12-10 | 四川大学华西医院 | Small intestinal submucosa-based vascularization promoting thermosensitive material and preparation method thereof |
| CN105412986A (en) * | 2015-12-14 | 2016-03-23 | 宁波大学 | SIS (small intestinal submucosa) medicine carrying patch as well as preparation method and application thereof |
| CN106456837A (en) * | 2014-03-21 | 2017-02-22 | 匹兹堡大学-联邦高等教育体系 | Methods for preparation of a terminally sterilized hydrogel derived from extracellular matrix |
| CN106729998A (en) * | 2017-04-14 | 2017-05-31 | 苏州医甸园医疗科技发展有限公司 | Artificial blood vessel's inside coating of sea-mussel mucin modification and preparation method and application |
-
2017
- 2017-07-06 CN CN201710546641.0A patent/CN107308495A/en not_active Withdrawn
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1559360A (en) * | 2004-02-19 | 2005-01-05 | 重庆大学 | Method for planting esoderma/endothelial cell on inner surface of artificial blood vessel |
| CN101002967A (en) * | 2006-12-27 | 2007-07-25 | 重庆大学 | Imaginal stem cell membrane stent in blood vessel, and its preparing method |
| CN106456837A (en) * | 2014-03-21 | 2017-02-22 | 匹兹堡大学-联邦高等教育体系 | Methods for preparation of a terminally sterilized hydrogel derived from extracellular matrix |
| CN104189009A (en) * | 2014-09-01 | 2014-12-10 | 四川大学华西医院 | Small intestinal submucosa-based vascularization promoting thermosensitive material and preparation method thereof |
| CN105412986A (en) * | 2015-12-14 | 2016-03-23 | 宁波大学 | SIS (small intestinal submucosa) medicine carrying patch as well as preparation method and application thereof |
| CN106729998A (en) * | 2017-04-14 | 2017-05-31 | 苏州医甸园医疗科技发展有限公司 | Artificial blood vessel's inside coating of sea-mussel mucin modification and preparation method and application |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Hu et al. | In vitro and in vivo evaluation of a small-caliber coaxial electrospun vascular graft loaded with heparin and VEGF | |
| Hu et al. | Mercerization of tubular bacterial nanocellulose for control of the size and performance of small-caliber vascular grafts | |
| Zhai et al. | Coaxial electrospinning of P (LLA‐CL)/heparin biodegradable polymer nanofibers: Potential vascular graft for substitution of femoral artery | |
| CN100364619C (en) | Artificial blood vessel silk fibroin and collagen blending pre-coagulation coating | |
| CN104524632B (en) | A kind of preparation method of the anticoagulation composite tube support with good conformability | |
| CN108478863A (en) | The preparation method and products thereof of compound small-caliber artificial blood vessel | |
| CN112675360B (en) | Preparation and application of hADSCs-loaded double-layer skin bionic hydrogel composite scaffold | |
| Zhu et al. | A fabric reinforced small diameter tubular graft for rabbits’ carotid artery defect | |
| Figliuzzi et al. | Renal bioengineering with scaffolds generated from rat and pig kidneys | |
| Zhang et al. | Evolution of biomimetic ECM scaffolds from decellularized tissue matrix for tissue engineering: A comprehensive review | |
| CN100364620C (en) | Artificial blood vessel collagen pre-coagulation coating | |
| CN103961752A (en) | Guided tissue regeneration membrane and preparation method thereof | |
| WO2016049625A1 (en) | Novel biofabrication techniques for the implementation of intrinsic tissue geometries to an in vitro collagen hydrogel | |
| CN111714703B (en) | High-compliance tissue engineering blood vessel preparation template and tissue engineering blood vessel | |
| CN107308495A (en) | A kind of artificial blood vessel based on trees-Osima jacoti, Osima excavata and preparation method thereof | |
| CN105748171B (en) | Biological nerve duct | |
| CN109289093B (en) | PGS/PCL double-layer artificial blood vessel with reticular sheath layer structure and construction method thereof | |
| KR20170096572A (en) | Extra-cellular matrix for burn wound and skin deficit healing and the method of thereof | |
| Liu et al. | Construction of functional magnetic scaffold with temperature control switch for long-distance vascular injury | |
| CN104524641B (en) | Poly-amino acid-based elastic vessel tissue engineering bracket and preparation method thereof | |
| Patra et al. | A novel route to polycaprolactone scaffold for vascular tissue engineering | |
| Li et al. | The proteins derived from platelet-rich plasma improve the endothelialization and vascularization of small diameter vascular grafts | |
| Wang et al. | Fabrication and performance evaluation of PLCL-hCOLIII small-diameter vascular grafts crosslinked with procyanidins | |
| CN107376037A (en) | Compound heparin biology anti-freezing coating solution and preparation method and application | |
| Ouyang et al. | In vivo histocompatibility evaluation of polyurethane membrane modified by superfine silk-fibroin powder |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20171103 |
|
| WW01 | Invention patent application withdrawn after publication |