CN101002967A - Imaginal stem cell membrane stent in blood vessel, and its preparing method - Google Patents
Imaginal stem cell membrane stent in blood vessel, and its preparing method Download PDFInfo
- Publication number
- CN101002967A CN101002967A CN 200610095366 CN200610095366A CN101002967A CN 101002967 A CN101002967 A CN 101002967A CN 200610095366 CN200610095366 CN 200610095366 CN 200610095366 A CN200610095366 A CN 200610095366A CN 101002967 A CN101002967 A CN 101002967A
- Authority
- CN
- China
- Prior art keywords
- sis
- progenitor cells
- lower floor
- rubber bar
- epc
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Abstract
An adult stem cells covered internal vascular scaffold is composed of metallic internal vascular scaffold, SIS covering on said scaffold, and adult stem cells planted on SIS. Its preparing process includes such steps as preparing bioactive SIS, separating the endothelial archeocytes from human umbilical blood, planting the archeocytes, and covering on said internal vascular scaffold.
Description
Technical field
The present invention relates to endovascular stent is carried out the overlay film modification and carries out the endothelialization processing,, belong to biologic medical technique with the apparatus field for the in-stent restenosis problem that causes after the prevention endovascular stent implantation provides new method.
Background technology
The importing of endovascular stent (intravascular stents) clinical practice is from percutaneous coronary artery endoluminal vascular plasty (percutaneous transluminal coronary angioplasty, PTCA) coronary artery is got involved the another important breakthrough in field after, because 60% the restenosis problem that support has solved that early stage blood vessel elasticity retraction and the negativity remodeling process in later stage cause.However, (in-stent restenosis ISR) limits by in-stent restenosis in the long-term success of endovascular stent.Though support can be eliminated blood vessel elasticity retraction and negativity remodeling process, support can be replied by the more obvious chronic blood vessel of the simple sacculus plasty of induction ratio again.Approximately ISR appears in the patient of 20-30%, needs multiple interventional therapy.Therefore, how to prevent the generation of ISR, research focus has become international.Carried out many correlational studyes both at home and abroad, develop drug-eluting stent ((drug-elutingstents, DES), inert coatings support, Biodegradable scaffold, cell or gene coating support etc., wherein DES has obtained good clinical effectiveness.
Because the limited biocompatibility of common metal support itself, not only arterial intima causes the delay of endothelialization process, and can not stop the outer tissue of support to the rack bore growth, and therefore many scholars are devoted to the research of overlay film frame.Overlay film frame (covered stent) constitutes coated with the special film material on the common metal inner support, and it had both kept the characteristic of common metal support, also had the characteristic property of institute's overlay film material.The outstanding feature of overlay film frame is: the membranous structure mechanicalness of its rack surface of mat intercepts the interaction of the inside and outside tissue of support.Mechanism from ISR, Fibrinogen in the blood, platelet, comprise that the interaction between VSMC, leukocyte and the excretory cytokine thereof is the main cause that causes neointimal hyperplasia in lymphocyte, macrophage and eosinophilic granulocyte's leukocyte and excretory cytokine and the vascular tissue, so the characteristic that overlay film frame had can be used to prevent the generation of ISR.In addition, if in the ISR process, the SMC of propagation derives from the hematopoietic stem cell of bone marrow really, and then the membranous structure of overlay film frame then can stop its intravasation wall and play the effect that prevents ISR.
The kind of report film-coated vascular inner rack mainly contains polymeric film support, medicine membrane support and biomembrane support at present, has obtained good experiment and clinical effectiveness in various degree, but has also still existed some problems.Increased the surface area that contacts with blood significantly as the polymeric film support,, can cause more serious thrombosis and smooth muscle cell proliferation, and the degradation with aging phenomenon of membrane material can occur if deal with improperly; In the medicine membrane support, still need further to inquire into and improve for problems such as the kind of medicine, drug loading, slow release characteristics; The biomembrane support is the most potential a kind of overlay film frame type, and membrane material mainly comprises autogenous vein, through the fibrin of the allosome vein of degenerative treatments, heparinization and type i collagen albumen etc.The autogenous vein overlay film frame has been obtained comparatively ideal effect at present, but its source is limited, and can damage body.Therefore, selecting ideal biological covering material is to improve the important main direction of studying of overlay film frame.
Summary of the invention
The objective of the invention is to research and develop a kind of new biomembrane endovascular stent, limited and source approach may cause the present situation of certain injury to human body at present biomembrane support covering material source, a kind of pig intestinal mucosa lower floor's endovascular stent and preparation method are proposed, a kind of source approach of new biomembrane material is provided, and with its carrier that is adult stem cell is grown endovascular stent is carried out endothelialization and modify, promote endovascular stent to implant back blood vessel injury region endothelialization process again, the generation of prevention ISR.
The submucous layer of small intestine of pig (Small intestinal submucosa, SIS) be a kind of cell component that do not contain, non-immunogenicity, biodegradable, based on the n cell epimatrix of collagen composition (referring to document: Lantz GC, Badylak SF, Hiles MC, et al. submucous layer of small intestine is as blood vessel graft .J Invest Surg 1993; 6:297-310).Remove the mucous layer and the outside flesh layer and the placenta percreta of pig small intestine with physical method, can obtain SIS (referring to document: Abraham G A through taking off cell and sterilization, sterilization treatment again, Murray J, Billiar K, et al. Intestinum Sus domestica collagen layer is as the evaluation .J Biomed MaterRes as biomaterial, 2000,51:442-452).SIS comprises various kinds of cell epimatrix molecule, fibronectin (fibronectin, FN) hyaluronic acid (hyaluronic acid, HA) heparin (heparin) for example, chondroitin sulfate A and B (chondroitin sulfates Aand B), Heparan sulfate (heparan sulfate).In addition, SIS also contains multiple somatomedin, as fibroblast growth factor (fibroblast growth factor, FGF), transforming growth factor-beta (Transforming growth factor, TGF-β), VEGF (Vascular endothelial growth factor, VEGF) (referring to document: Elias Brountzos, Dusan Pavcnik, the submucous layer of small intestine venous valve that Hans A.et al. suspends reinvent an experimental research .J.Vasc.Interv.Radiol. who estimates about the host cell source, Mar 2003; 14:349.).Studies show that SIS is of value to tissue reconstruction.In degraded and heavy absorption process, it can be reinvented by host tissue.Recently U.S. FDA (the United States Food andDrug Administration) has been agreed the biomaterial as soft tissue repair and skin trauma dressing with SIS.
Ins and outs in view of SIS, main contents of the present invention are for making up a kind of Imaginal stem cell membrane stent in blood vessel, it by endovascular stent, be overlying on the SIS on the endovascular stent and be planted in endothelial progenitor cells on the SIS that (Endothelial ProgenitorCells EPC) constitutes.Endovascular stent is rustless steel (316L) support, nick-eltitanium alloy stent, cobalt-base alloys support or biodegradable magnesium alloy support; Submucous layer of small intestine SIS is the pig jejunal submucosa; Endothelial progenitor cells EPC is people's umbilical blood endothelial progenitor cells or human peripheral endothelial progenitor cells.
Technical thought of the present invention is the SIS that at first prepares biologically active, separation of human umbilical blood endothelial progenitor cells, use the organizational project principle then, carry out cell seeding, make it form the lining of endothelial progenitor cells, by certain technology it is covered again and modify endovascular stent, make the support inner surface form smooth, a smooth thin layer, strengthen the biocompatibility and the blood compatibility of endovascular stent.
Concrete steps are as follows:
The preparation of 1SIS: the SIS preparation method is referring to document: Abraham G A, Murray J, Billiar K, et al. Intestinum Sus domestica collagen layer is as the evaluation .J Biomed Mater Res of biomaterial, and 2000,51:442-452. opens just, CengBing Fang, Zhang Changqing. the experimentation of the compound submucous layer of small intestine external structure of bone marrow stroma stem cell organizational project periosteum. Chinese surgical magazine, 2005,43 (24): 1594-1597.
The separation and cultivation of 2 people's umbilical blood endothelial progenitor cells: people's umbilical blood endothelial progenitor cells separate with cultural method referring to document: Yi Chenggang, Guo Shuzhong, Zhang Linxi, etc. the experimentation of people's umbilical blood medium vessels endothelial progenitor cells amplification in vitro and evaluation. Chinese aesthetic medicine, 2004,13 (1): 22-24.
3 carry out the endothelial progenitor cells plantation on SIS, acquisition is loaded with the SIS of endothelial progenitor cells: plant method for planting referring to document: BadylakS, Liang A, Record R, Tullius R, Hodde J. inner skin cell viscosity attached primary school intestinal submucosa: a kind of acellular biological engineering support .Biomaterials 1999; 20:2257-2263
4SIS film-coated vascular inner rack manufacture method: need with material rubber bar, endovascular stent are arranged, are loaded with the pig SIS, nitrocellulose filter of EPC etc., manufacturing process is as follows:
1) manufacture method of inner chamber overlay film frame
1. select the rubber bar suitable with the endovascular stent internal diameter; Accurately cutting is loaded with the pig SIS (thickness is about 180-200 μ m) of EPC, makes its width slightly be longer than rubber bar girth 0.5～1.0mm, and the length of length and endovascular stent is equal to;
2. will be loaded with the pig SIS parcel rubber bar of EPC, the cell face of SIS is contacted with rubber bar, and adopt degradable prolene monofilament, the method for sewing up is stitched into tubulose continuously;
3. step is enclosed with the rubber bar of SIS in 2., inserts the endovascular stent inner chamber, the method that reuse is sewed up continuously is overlying on rack bore with the pig SIS that is loaded with EPC;
4. the support of step in 3. immersed in the PBS solution together with rubber bar, rubber bar is separated with SIS.
2) manufacture method of outer surface overlay film frame
1. select the rubber bar suitable with the endovascular stent external diameter, accurately cutting is loaded with the pig SIS (thickness is about 180-200 μ m) of EPC, makes its width slightly be longer than rubber bar girth 0.5～1.0mm, and the length of length and endovascular stent is equal to;
2. will be loaded with the pig SIS parcel rubber bar of EPC, the cell face of SIS is contacted with rubber bar, and adopt degradable prolene monofilament, the method for sewing up is stitched into tubulose continuously;
3. the rubber bar that step is enclosed with SIS in 2. immerses in the PBS solution, and rubber bar is separated with SIS;
4. the SIS pipe that the endovascular stent inserting step is obtained in 3. adopts degradable prolene monofilament, and the method with sewing up continuously makes the pig SIS that is loaded with EPC be overlying on the endovascular stent outer surface.
Major advantage of the present invention is: 1. proposed a kind of film material source of new overlay film frame, SIS is non-immunogenicity not only, and is of value to the reconstruction of damaged tissue, has overcome the shortcoming of existing biofilms material; 2. SIS can carry out the plantation of endothelial progenitor cells or mesenchymal stem cells MSCs, makes it be divided into vascular endothelial cell on amnion stroma, promotes the endovascular stent damage location purpose of endothelialization again to reach.
Description of drawings
Below in conjunction with accompanying drawing the present invention is further detailed:
Fig. 1: the SIS structural representation that is loaded with endothelial progenitor cells.
Fig. 2: inner chamber covers the process flow diagram of the SIS endovascular stent that is loaded with endothelial progenitor cells.
Fig. 3: outer surface covers the process flow diagram of the SIS endovascular stent that is loaded with endothelial progenitor cells.
Fig. 4: inner chamber covers the sketch map of SIS endovascular stent.
Fig. 5: outer surface covers the sketch map of SIS endovascular stent.
The specific embodiment
The preparation of the first step: SIS
1. physical treatment method: get the fresh pig small intestinal of butchering in the 4h, the placenta percreta and the flesh layer of small intestinal are removed with the handle of a knife that is wrapped with gauze, clean with 40 ℃ of water continual rinsings.
2. the small intestinal of handling with physical method is at room temperature handled through a series of chemical method.The volume ratio of material and solution remained on 1: 100.
1) SIS that mechanical means is made is immersed in and contains (pH11～12) 16h in 100mmol/L ethylenediaminetetraacetic acid (EDTA) and the 10mmol/LNaOH solution;
2) with deionized water substrate is rinsed well, (pH0～1) soaks 6～8h in containing 1mmol/L HCl and 1mmol/L NaCl solution;
3), in the phosphate buffer (PBS) of 1mmol/L NaCl, soak 16h with after the deionized water rinsing substrate;
4) with after the deionized water rinsing substrate, (pH7～7.4) soak 2h in PBS solution;
5) reuse deionized water rinsing substrate 2h (pH5.8～7.0);
6) sterilization: SIS is soaked 8h in containing 20% alcoholic solution of 0.1% peracetic acid, cleans 2h with the PBS solution that contains 0.05% Hydrazoic acid,sodium salt, then-70 after ℃ lyophilizing with radiation gamma (25～35kGy) sterilizations.
Second step: the separation and Culture of people's umbilical blood endothelial progenitor cells:
1.EPC separation: people's umbilical blood is all taken from the puerpera of mature health, every part the blood sampling about 40-60ml, compound recipe sodium citrate injection (ACD-A) by 1: 10 the adding anticoagulant.Add the PBS dilution then at 1: 1, EPC separates and adopts density: the umbilical blood of dilution is added Histopaque-1077 cell separation liquid, with 400g/min at the centrifugal 30min of room temperature, sucking-off mononuclear cell layer.Clean with PBS then and count after 3 cells are blown out suspension.
2.EPC cultivation: isolating mononuclear cell is with 1-3 * 10 in the umbilical blood
5Density be inoculated in and be covered with in fibronectin (FN) plastic culture dish, culture fluid M-199, contain 20% hyclone, Medulla Bovis seu Bubali hypophysis extract (BPE), the heparin of 100 μ g/ml, put into 37 ℃, the cell culture incubator of 5%CO2, humidity 100% is respectively at changing liquid in the 4th, 7,10 day that cultivates.
3.EPC evaluation: 6 isolating mononuclear cells are with 1-3 * 10 in the umbilical blood
5Density be inoculated in the culture plate that is placed with the coverslip that is coated with FN, take out cell climbing sheet respectively at the 4th, 7,10 day that cultivates, the SABC method detects the expression of CD31, CD34 and KDR, immunofluorescence detects the expression of CD133.
The 3rd step: the endothelial progenitor cells modification of SIS: with the endothelial progenitor cells cultivated in second step in the 5%CO2 incubator 37 ℃ hatch and cultivate and go down to posterity.The SIS for preparing after PBS solution aquation, is dripped the 10% calf serum 10min that prewets, and sucking-off discards.With endothelial progenitor cells 0.25% trypsinization that goes down to posterity and cultivate, make cell suspension, with 2.0～3.0 * 10
4The cell density of individual/ml drips 2ml on the SIS for preparing.Culture fluid is inserted 37 ℃, 5%CO then
2Cultivate in the incubator.Fig. 1 is the SIS structural representation that is loaded with endothelial progenitor cells of acquisition, and the upper strata is endothelial progenitor cells EPC 2, and lower floor is submucous layer of small intestine SIS 3.
The 4th step: the structure of Imaginal stem cell membrane stent in blood vessel:
Need with material rubber bar, endovascular stent are arranged, are loaded with the pig SIS, nitrocellulose filter of EPC etc., in strict accordance with aseptic principle, institute all operates in superclean bench in steps, manufacturing process is as follows:
1. the manufacture method of inner chamber overlay film frame, referring to Fig. 2:
1. select the rubber bar 5 suitable with metallic blood vessel inner support 1 internal diameter; Accurately cutting is loaded with the pig SIS 4 (thickness is about 180-200 μ m) of EPC, makes its width slightly be longer than rubber bar 5 girths 0.5～1.0mm, and the length of length and metallic blood vessel inner support 1 is equal to;
2. will be loaded with the pig SIS 4 parcel rubber bars 5 of EPC, the cell face of SIS is contacted with rubber bar, and adopt degradable prolene monofilament, the method for sewing up is stitched into tubulose continuously;
3. step is enclosed with the rubber bar 5 of SIS in 2., inserts metallic blood vessel inner support 1 inner chamber, the method that reuse is sewed up continuously is overlying on rack bore with the pig SIS that is loaded with EPC;
4. the support of step in 3. immersed in the PBS solution together with rubber bar, rubber bar is separated with SIS, obtain inner chamber overlay film frame 6 as shown in Figure 4.
2. the manufacture method of outer surface overlay film frame, referring to Fig. 3:
1. select the rubber bar 5 suitable with metallic blood vessel inner support 1 external diameter, accurately cutting is loaded with the pig SIS 4 (thickness is about 180-200 μ m) of EPC, makes its width slightly be longer than rubber bar 5 girths 0.5～1.0mm, and the length of length and metallic blood vessel inner support 1 is equal to;
2. will be loaded with the pig SIS4 parcel rubber bar 5 of EPC, the cell face of SIS is contacted with rubber bar, and adopt degradable prolene monofilament, the method for sewing up is stitched into tubulose continuously;
3. the rubber bar 5 that step is enclosed with SIS in 2. immerses in the PBS solution, and rubber bar is separated with SIS;
4. the SIS pipe that endovascular stent 1 inserting step is obtained in 3., adopt degradable prolene monofilament, with the method for continuous stitching, make the pig SIS that is loaded with EPC be overlying on metallic blood vessel inner support outer surface, obtain outer surface overlay film frame 7 as shown in Figure 5.
Certainly, in this example, also can adopt the human peripheral endothelial progenitor cells is repopulating cell, and intravascular stent also can adopt nick-eltitanium alloy stent, cobalt-base alloys support or biodegradable magnesium alloy support etc., preparation according to the method described above can obtain the overlay film frame that needs equally.
Claims (4)
1, a kind of Imaginal stem cell membrane stent in blood vessel is characterized in that: it by endovascular stent (1), cover the submucous layer of small intestine (SIS) (2) on the endovascular stent and the adult stem cell endothelial progenitor cells (EPC) (3) that is planted on the submucous layer of small intestine (SIS) constitutes; Endovascular stent (1) is stainless steel stent, nick-eltitanium alloy stent, cobalt-base alloys support or biodegradable magnesium alloy support; Submucous layer of small intestine (SIS) (2) is the pig jejunal submucosa; Endothelial progenitor cells (EPC) (3) is people's umbilical blood endothelial progenitor cells or human peripheral endothelial progenitor cells.
2, the method for preparing the described submucous layer of small intestine film-coated vascular inner rack of claim 1, its step is as follows:
1. the submucous layer of small intestine (SIS) for preparing pig;
2. go up plantation endothelial progenitor cells (EPC) at submucous layer of small intestine (SIS), obtain to be loaded with the pig intestinal mucosa lower floor (SIS) of endothelial progenitor cells (EPC);
3. cover with the pig intestinal mucosa lower floor (SIS) that is loaded with endothelial progenitor cells (EPC) and modify endovascular stent, make inner chamber overlay film frame or outer surface overlay film frame.
3, method according to claim 2 is characterized in that: the step that is 3. obtained the inner chamber overlay film frame by step is as follows:
1. select the rubber bar suitable with the endovascular stent internal diameter; The pig intestinal mucosa lower floor (SIS) that accurate cutting is loaded with endothelial progenitor cells (EPC) makes its width slightly be longer than rubber bar girth 0.5～1.0mm, and the length of length and endovascular stent is equal to;
2. will be loaded with pig intestinal mucosa lower floor (SIS) the parcel rubber bar of endothelial progenitor cells (EPC), the cell face of pig intestinal mucosa lower floor (SIS) is contacted with rubber bar, and adopt degradable prolene monofilament, the method for sewing up is stitched into tubulose continuously;
3. step is enclosed with the rubber bar of pig intestinal mucosa lower floor (SIS) in 2., inserts the endovascular stent inner chamber, the method that reuse is sewed up continuously, the pig intestinal mucosa lower floor (SIS) that will be loaded with endothelial progenitor cells (EPC) is overlying on rack bore;
4. the support of step in 3. immersed in the PBS solution together with rubber bar, rubber bar is separated with pig intestinal mucosa lower floor (SIS), obtain the inner chamber overlay film frame.
4, method according to claim 2 is characterized in that: the step that is 3. obtained the outer surface overlay film frame by step is as follows:
1. select the rubber bar suitable with the endovascular stent external diameter, accurately cutting is loaded with the pig intestinal mucosa lower floor (SIS) of endothelial progenitor cells (EPC), makes its width slightly be longer than rubber bar girth 0.5～1.0mm, and the length of length and endovascular stent is equal to;
2. will be loaded with pig intestinal mucosa lower floor (SIS) the parcel rubber bar of endothelial progenitor cells (EPC), the cell face of pig intestinal mucosa lower floor (SIS) is contacted with rubber bar, and adopt degradable prolene monofilament, the method for sewing up is stitched into tubulose continuously;
3. the rubber bar that step is enclosed with pig intestinal mucosa lower floor (SIS) in 2. immerses in the PBS solution, and rubber bar is separated with pig intestinal mucosa lower floor (SIS);
4. in pig intestinal mucosa lower floor (SIS) pipe that the endovascular stent inserting step is obtained in 3., adopt degradable prolene monofilament, with the method for continuous stitching, make the pig intestinal mucosa lower floor (SIS) that is loaded with endothelial progenitor cells (EPC) be overlying on the endovascular stent outer surface.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006100953667A CN100475279C (en) | 2006-12-27 | 2006-12-27 | Imaginal stem cell membrane stent in blood vessel, and preparing method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006100953667A CN100475279C (en) | 2006-12-27 | 2006-12-27 | Imaginal stem cell membrane stent in blood vessel, and preparing method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101002967A true CN101002967A (en) | 2007-07-25 |
| CN100475279C CN100475279C (en) | 2009-04-08 |
Family
ID=38702489
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2006100953667A Expired - Fee Related CN100475279C (en) | 2006-12-27 | 2006-12-27 | Imaginal stem cell membrane stent in blood vessel, and preparing method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN100475279C (en) |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101185774B (en) * | 2007-10-25 | 2011-06-08 | 王振军 | Medical bioavailability bracket material, preparation method and uses thereof |
| CN101366977B (en) * | 2008-09-03 | 2013-07-24 | 陕西瑞盛生物科技有限公司 | Tissue mending material with biological activity and preparation method thereof |
| CN104127266A (en) * | 2014-06-16 | 2014-11-05 | 苏州固基电子科技有限公司 | Drug-loading vascular stent |
| CN105664258A (en) * | 2016-02-22 | 2016-06-15 | 江苏期佰医疗技术有限公司 | Method for preparing smooth muscle cell carrier based on small intestine submucosa acellular matrix |
| CN106963986A (en) * | 2017-01-17 | 2017-07-21 | 宁波大学 | Fat stem cell ECM modification SIS integration engineering bones and preparation method thereof |
| CN107029296A (en) * | 2017-03-03 | 2017-08-11 | 北京博辉瑞进生物科技有限公司 | Periosteum patch, the preparation method and application of a kind of Guided Bone Regeneration |
| CN107308495A (en) * | 2017-07-06 | 2017-11-03 | 苏州期佰生物技术有限公司 | A kind of artificial blood vessel based on trees-Osima jacoti, Osima excavata and preparation method thereof |
| CN110292664A (en) * | 2018-03-21 | 2019-10-01 | 美敦力瓦斯科尔勒公司 | Tissue coating product |
-
2006
- 2006-12-27 CN CNB2006100953667A patent/CN100475279C/en not_active Expired - Fee Related
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101185774B (en) * | 2007-10-25 | 2011-06-08 | 王振军 | Medical bioavailability bracket material, preparation method and uses thereof |
| CN101366977B (en) * | 2008-09-03 | 2013-07-24 | 陕西瑞盛生物科技有限公司 | Tissue mending material with biological activity and preparation method thereof |
| CN104127266A (en) * | 2014-06-16 | 2014-11-05 | 苏州固基电子科技有限公司 | Drug-loading vascular stent |
| CN105664258A (en) * | 2016-02-22 | 2016-06-15 | 江苏期佰医疗技术有限公司 | Method for preparing smooth muscle cell carrier based on small intestine submucosa acellular matrix |
| CN106963986A (en) * | 2017-01-17 | 2017-07-21 | 宁波大学 | Fat stem cell ECM modification SIS integration engineering bones and preparation method thereof |
| CN107029296A (en) * | 2017-03-03 | 2017-08-11 | 北京博辉瑞进生物科技有限公司 | Periosteum patch, the preparation method and application of a kind of Guided Bone Regeneration |
| CN107029296B (en) * | 2017-03-03 | 2020-09-29 | 北京博辉瑞进生物科技有限公司 | Periosteum repairing piece for guiding bone regeneration, preparation method and application |
| CN107308495A (en) * | 2017-07-06 | 2017-11-03 | 苏州期佰生物技术有限公司 | A kind of artificial blood vessel based on trees-Osima jacoti, Osima excavata and preparation method thereof |
| CN110292664A (en) * | 2018-03-21 | 2019-10-01 | 美敦力瓦斯科尔勒公司 | Tissue coating product |
Also Published As
| Publication number | Publication date |
|---|---|
| CN100475279C (en) | 2009-04-08 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN100475279C (en) | Imaginal stem cell membrane stent in blood vessel, and preparing method thereof | |
| Jana | Endothelialization of cardiovascular devices | |
| Jaganathan et al. | Biomaterials in cardiovascular research: applications and clinical implications | |
| Tschoeke et al. | Tissue-engineered small-caliber vascular graft based on a novel biodegradable composite fibrin-polylactide scaffold | |
| Dimitrievska et al. | Historical perspective and future direction of blood vessel developments | |
| Avci-Adali et al. | New strategies for in vivo tissue engineering by mimicry of homing factors for self-endothelialisation of blood contacting materials | |
| Campbell et al. | Development of tissue engineered vascular grafts | |
| Bordenave et al. | Clinical performance of vascular grafts lined with endothelial cells | |
| ES2347339T3 (en) | ENGINEERING WITH GUIDING FACTORS. | |
| Cai et al. | Selection of different endothelialization modes and different seed cells for tissue-engineered vascular graft | |
| Shtilʹman | Polymeric biomaterials | |
| JP2005531355A (en) | Decellularized tissue | |
| Jantzen et al. | Use of autologous blood-derived endothelial progenitor cells at point-of-care to protect against implant thrombosis in a large animal model | |
| Zhou et al. | Development of decellularized aortic valvular conduit coated by heparin–SDF-1α multilayer | |
| CN105228665A (en) | Antithrombus formation graft | |
| Di Franco et al. | Biomaterials and heart recovery: cardiac repair, regeneration and healing in the MCS era: a state of the “heart” | |
| Cho et al. | Evidence for in vivo growth potential and vascular remodeling of tissue-engineered artery | |
| Zhang et al. | Hematopoietic stem cell capture and directional differentiation into vascular endothelial cells for metal stent-coated chitosan/hyaluronic acid loading CD133 antibody | |
| CN111603611A (en) | Cell-derived matrix tubular scaffold and preparation method thereof | |
| Sugimura et al. | A rat model for the in vivo assessment of biological and tissue-engineered valvular and vascular grafts | |
| CN101195047A (en) | Antithrombotic and restenosis blood vessel bracket and method for preparing the same | |
| Hessenauer et al. | Watching the vessels grow: establishment of intravital microscopy in the arteriovenous loop rat model | |
| Rodgers et al. | The coronary artery response to implantation of a balloon-expandable flexible stent in the aspirin-and non-aspirin-treated swine model | |
| Betala et al. | Drug-coated percutaneous balloon catheters | |
| Avci‐Adali et al. | Application of a rotating bioreactor consisting of low‐cost and ready‐to‐use medical disposables for in vitro evaluation of the endothelialization efficiency of small‐caliber vascular prostheses |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20090408 Termination date: 20151227 |
|
| EXPY | Termination of patent right or utility model |