CN107290525A - A kind of chemiluminescence detection kit of sulphadiazine and preparation method thereof - Google Patents
A kind of chemiluminescence detection kit of sulphadiazine and preparation method thereof Download PDFInfo
- Publication number
- CN107290525A CN107290525A CN201710726759.1A CN201710726759A CN107290525A CN 107290525 A CN107290525 A CN 107290525A CN 201710726759 A CN201710726759 A CN 201710726759A CN 107290525 A CN107290525 A CN 107290525A
- Authority
- CN
- China
- Prior art keywords
- sulphadiazine
- chemiluminescence
- detection kit
- chemiluminescence detection
- antibody
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
- G01N33/54326—Magnetic particles
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/76—Chemiluminescence; Bioluminescence
Landscapes
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Pathology (AREA)
- Hematology (AREA)
- Molecular Biology (AREA)
- Urology & Nephrology (AREA)
- General Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Food Science & Technology (AREA)
- Cell Biology (AREA)
- Microbiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Plasma & Fusion (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
The invention discloses a kind of chemiluminescence detection kit of sulphadiazine and preparation method thereof.The kit includes：Coupling has magnetic particle, acridinium ester label, sulphadiazine serial standards, chemiluminescence preexciting liquid A, chemiluminescence exciting liquid B, the cleaning fluid of sulphadiazine antigen or antibody.Compared with the technology of existing detection sulphadiazine, this kit has sensitivity high, and signal to noise ratio is high, the advantage of quick detection.Its sensitivity for analysis is up to 0.030 μ g/L.
Description
Technical field
The present invention relates to technical field of food detection, the chemiluminescence detection reagent of sulphadiazine in specifically a kind of food
Box and preparation method thereof.
Background technology
Sulphadiazine, also known as Sulfadiazine, its structure is：
Sulphadiazine is one of maximum sulfa drugs of usage amount, and antibacterial action is strong, as the antimicrobial in veterinary drug, is often incorporated
In the miscellaneous material to word, the infectious diseases for treating animal.The unreasonable of sulphadiazine uses, and becomes residue problem most tight
The veterinary drug of weight.The animal derived food containing such medicine is used for a long time in people, can cause the normal thalline in body is produced resistance to
The property of medicine causes people's poisoning, allergic reaction, or even causes the generation of cancer.The residual quantity of sulphadiazine has been subjected to various countries
Pay attention to.Therefore, 100 μ g/kg are limited to maximum permission quantity of the sulfa drugs in meat and dairy products both at home and abroad, wherein
The residual limit amount of highest that Japan requires is more strict, and single sulfa drug residue limit amount is 20 μ g/kg, and maximum residual is total
Amount must not exceed 100 μ g/kg.
At present, the method for detection sulphadiazine has high performance liquid chromatography, enzyme linked immunosorbent assay, colloidal gold method.Wherein,
Liquid phase chromatographic analysis method lacks highly sensitive detector, and instrumentation is cumbersome, complex pretreatment, takes.Enzyme-linked immunoassay method, is adopted
With HRPO or alkaline phosphatase marker, its enzyme marker easy in inactivation, chromogenic substrate is shown in that light is easily decomposed, sensitivity
Low, the compound similar to structure has certain cross reaction, causes test result inaccurate.
CN 1766631A（2006.05）Sulphur has been carried out to animal tissue, honey, urine and milk using ELISA
Amine drug is detected that its kit is for Cleaning Principle, with sulfa drugs antigen or antibody with competitiveness enzyme-linked reaction
Coated elisa plate, surveys OD values come result of determination, it is inaccurate to easily cause test result, and its sensitivity after reaction by developing the color
Low, operating process is cumbersome.
CN 1807601 A（2006.08）Using sulfanilamide (SN) in colloidal gold method detection tissue, honey, milk and egg sample
The residual quantity of pyrimidine, the last testing result of its kit is characterized with macroscopic color, and error is larger, and operates
Cumbersome, flow is more, is more easy to error occur, causes test result inaccurate.And a kind of sulphadiazine chemistry of our company's exploitation
Luminescence reagent box, using acridinium ester as label, has the advantages that detection is stable, measurement is quick, sensitivity is high.
Chemiluminescence method is compared with the simplicity that the advantage of other method is to detect.
The system uses Magneto separate system, and the advantage that the chemiluminescence of acridinium ester label is detected is：Acridinium ester
The presence of catalyst is not required to as luminous agent, can be lighted in the dilute alkaline soln for have hydrogen peroxide；Acridinium ester molecular weight is small, keeps away
Exempt to cover antibody combining site, system overall sensitivity can be improved, be swift in response；Background is low, signal to noise ratio is high, is that a class is effective
Chemiluminescent labels.
The content of the invention
It is an object of the invention to provide one kind is stable, detection speed is fast, with higher sensitivity and specific immune
Magnetic microparticle chemiluminescence detection method, to detect that sulphadiazine provides facility in food.
To achieve the above object, the present invention is adopted the following technical scheme that：
A certain amount of sample to be tested is added in reaction cup, magnetic particle coupling suspension and acridinium ester label is then added, mixes
It is even, it is incubated several minutes in 37 DEG C, is finally separating, washs, add chemiluminescence preexciting liquid A and exciting liquid B, determines corresponding hair
Luminous intensity, compares sulphadiazine examination criteria curve, obtains the concentration for the sulphadiazine treated in test sample.
Chemiluminescent labels in the present invention are acridinium ester, such as NSP-DMAE-NHS, NSP-SA-NHS.
Acridinium ester label in the present invention is included：Acridinium ester label is sulphadiazine antigen or antibody, wherein acridine
The concentration range of ester solution is 2-85 mmol/L, and its preferred concentration is 6.5 mmol/L.
Buffer solution is pH 4.5-6.5 in the present invention, and concentration is 0.1 mol/L, and the MES by 0.22 μm of filter filtering delays
Fliud flushing.
Heretofore described sulphadiazine series of calibration product concentration is respectively：0 μg/L、0.02 μg/L、0.12 μg/
L, 0.72 μ g/L, 4.32 μ g/L, 26.0 μ g/L, its buffer solution are containing 0.5-5.0% BSA and 0.1-0.5% PC300
Tris-HCl。
Chemiluminescence preexciting liquid A in the present invention is：H2O2And HNO3Mixed liquor, wherein H2O2Mass fraction be
0.05-5% mol/L, HNO3Concentration is 0.05-2.5 mol/L.
Chemiluminescence exciting liquid B in the present invention is：Triton X-100 and NaOH mixed liquor, wherein Triton X-
100 concentration is that 0.05-2.0 mol/L, NaOH concentration are 0.05-1.0 mol/L.
Cleaning fluid in the present invention is：PH 7.0-9.0, concentration are 5.0-50.0 mmol/L Tris-HCl solution, its
In containing concentration be 0.05-0.50 mol/L NaCl and 0.01-0.25% Tween-20.
Compared with prior art, the advantage of the invention is that：The stabilization of kit of the present invention, detection are fast, sensitivity is high and
Specificity is good.
Embodiment
The technical scheme in the present invention will clearly and detailedly be illustrated below.Experimental method is such as without special in example
Illustrate, be conventional method.
Embodiment 1：The establishment of kit 1 and its preparation of concrete component
1. the establishment of kit
A kind of chemiluminescence detection kit of sulphadiazine, makes it contain following component：
The sulphadiazine antigen of acridinium ester label；
Carboxyl magnetic bead is coupled sulphadiazine antibody；
Chemiluminescence preexciting liquid A and chemiluminescence exciting liquid B；
Sulphadiazine serial standards solution, standard concentration is respectively：0 μg/L、0.02 μg/L、0.12 μg/L、0.72 μ
G/L, 4.32 μ g/L, 26.0 μ g/L, its buffer solution is the Tris- containing 0.5-5.0% BSA and 0.1-0.5% PC300
HCl；
Cleaning fluid, specially pH 7.2, concentration are 25 mmol/L Tris-HCl solution, wherein being 0.15 mol/L containing concentration
NaCl and 0.05% Tween-20.
2. the preparation of magnetic bead coupled antibody
（1）1 mg carboxyls magnetic particle is taken in 0.5 mL centrifuge tubes, a certain amount of 0.1 mol/L MES buffer solutions are added, is vortexed mixed
It is even, it is placed on magnetic frame, standing 5 min makes magnetic particle be separated with liquid, abandoning supernatant is washed 3 times, added a certain amount of
MES（PH value is 6.0）Buffer solution, is vortexed.
（2）Add 15 μ L（15 μg）Sulphadiazine antibody, is vortexed, and revolving reaction pipe is incubated at room temperature 30 min.
（3）Add the coupling reagent EDC that 10 μ L concentration are 10 mg/mL to be vortexed, revolving reaction pipe is incubated at room temperature 2 h.
（4）Supernatant is removed, a certain amount of cleaning buffer solution is added（TBS+0.05%Tween-20）, wash 3 times.
（5）Closed with the buffer solution containing 1%BSA, repeatedly closing 4 times, every time 10 min.By the magnetic particle suspension
It is placed in 2-8 DEG C of preservation.
3. the preparation of MES buffer solutions（0.1 mol/L）
Take in 19.52 g MES solids, the deionized water for being dissolved in 500 mL, it is 6.0 to arrive pH with 1 mol/L NaOH regulations；
The solution mixed up is transferred in 1 L volumetric flask, constant volume.
4. coupling reagent EDC preparation（Concentration is 10 mg/mL）
1 g EDC is taken in 50 mL beaker, the MES buffer solutions of precooling are added, stirring treats its solution transfer to 100 mL's
In volumetric flask, constant volume.
5. the preparation of TBS-T buffer solutions（Concentration is 25 mmol/L）
Weigh in 3.05 g Tris, 8.775 g NaCl to 500 mL beaker, add 1mL 0.05% Tween-20, magnetic
In being 7.2, the volumetric flask for moving to 1000 mL with 6 mol/L HCl regulation pH under conditions of power stirring, constant volume.
6. the preparation of acridinium ester label antigen
（1）A certain amount of sulphadiazine is placed in bag filter, and bag filter is placed in the mark buffer solution not less than 1 L thoroughly
Analysis, during which buffer solution is at least changed 3 times, last time dialysed overnight, mark buffer solution be pH 9.5, concentration be 0.1 mol/L
Na2CO3-NaHCO3Buffer solution.
（2）1.7 mg acridinium ester NSP-DMAE-NHS is weighed, is dissolved in 447 μ L anhydrous dimethyl formamides DMF, matches somebody with somebody
Into 6.5 mmol/L NSP-DMAE-NHS DMF solutions.
（3）Antigenic solution after dialysis is placed in 500 μ L centrifuge tubes, a certain amount of 6.5 mmol/L NSP- is added
The molar ratio of DMAE-NHS DMF solutions, acridinium ester and antigen is 10:1, add 200 μ L mark buffer solutions, room temperature reaction 45
Min, adds the μ L of 10 g/L lysines 100, continues to react 15 min, makes mark reaction terminating.
（4）Label NSP-DMAE-NHS-Ag and free NSP-DMAE-NHS passes through Sephadex G-50 posts（1×
25cm）Separation, the PBS for being 0.1mol/L with purification buffer pH 6.3, concentration is balanced and is eluted chromatographic column.
（5）Protein peak is detected with chromatograph in separation process, the chemiluminescence intensity and 280 nm of efflux are measured respectively
Absorbance.
（6）Shading value height and the big eluent of absorbance are collected, 1%BSA is added（Volume）After dispense stored frozen.
7. the preparation of elution buffer（PH is the Na that 9.5, concentration is 0.1mol/L2CO3-NaHCO3）
A liquid（0.1 mol/L, Na2CO3）：Take 10.62 g Na2CO3Plus distilled water is to 1 L,
B liquid（0.1 mol/L, NaHCO3）：Take 8.4 g NaHCO3Plus distilled water is to 1 L,
The mL of A liquid 3 mL, B liquid 7 is taken to mix, i.e. A liquid：B liquid=3:7 ratio mixing.
8. chemiluminescence exciting liquid A, B preparation
（1）Chemiluminescence preexciting liquid A is by H2O2And HNO3Composition.Wherein, H2O2Mass fraction is 1.5%, HNO3Concentration is 0.1
Mol/L, 20 mL/ branch is distributed into brown bottle, 2-8 DEG C saves backup.
（2）Chemiluminescence exciting liquid B is made up of Triton X-100 and NaOH mixed liquor.Wherein, Triton X-100
Concentration is 0.1 mol/L, and NaOH concentration is 0.35 mol/L, 20 mL/ branch is distributed into brown bottle, 2-8 DEG C saves backup.
Embodiment 2：The establishment of kit 2 and its preparation of component
1. the establishment of kit
A kind of chemiluminescence detection kit of sulphadiazine, makes it contain following component：
The sulphadiazine antibody of acridinium ester label；
Carboxyl magnetic bead is coupled sulphadiazine antigen；
Chemiluminescence preexciting liquid A and chemiluminescence exciting liquid B；
Sulphadiazine serial standards solution, standard concentration is respectively：0 μg/L、0.02 μg/L、0.12 μg/L、0.72 μ
G/L, 4.32 μ g/L, 26.0 μ g/L, its buffer solution is the Tris- containing 0.5-5.0% BSA and 0.1-0.5% PC300
HCl；
Cleaning fluid, specially pH 7.2, concentration are 25 mmol/L Tris-HCl solution, wherein being 0.15 mol/L containing concentration
NaCl and 0.05% Tween-20.
2. the preparation of magnetic bead coupled antigen
（1）1mg carboxyls magnetic particle is taken in 0.5 mL centrifuge tubes, a certain amount of 0.1 mol/L MES buffer solutions are added, is vortexed mixed
It is even, it is placed on magnetic frame, standing 5min makes magnetic particle be separated with liquid, abandoning supernatant is washed 3 times, adds a certain amount of MES
（PH value is 5.0）Buffer solution, is vortexed.
（2）Add 18 μ L（18 μg）Sulphadiazine antigen, be vortexed, revolving reaction pipe, be incubated at room temperature 30 min.
（3）Add the coupling reagent EDC that 10 μ L concentration are 10 mg/mL to be vortexed, revolving reaction pipe is incubated at room temperature 2 h.
（4）Supernatant is removed, 200 μ L cleaning buffer solution is added（TBS+0.05% Tween-20）, wash 3 times.
（5）Closed with the buffer solution containing 1%BSA, repeatedly closing 4 times, every time 10 min.By the magnetic particle suspension
It is placed in 2-8 DEG C of preservation.
3. the preparation of acridinium ester label antibody
（1）A certain amount of sulphadiazine antibody is placed in bag filter, and bag filter is placed in the mark buffer solution not less than 1 L
Middle dialysis, during which buffer solution at least change 3 times, last time dialysed overnight, mark buffer solution be that pH value is that 10.1, concentration is
0.1 mol/L Na2CO3-NaHCO3Buffer solution.
（2）1.7 mg acridinium ester NSP-DMAE-NHS is weighed, is dissolved in 447 μ L anhydrous dimethyl formamides DMF, matches somebody with somebody
Into 6.5 mmol/L NSP-DMAE-NHS DMF solutions.
（3）Antibody-solutions after dialysis are placed in 500 μ L centrifuge tubes, a certain amount of 6.5 mmol/L NSP- is added
The molar ratio of DMAE-NHS DMF solutions, acridinium ester and antibody is 7.4:1,200 μ L mark buffer solutions are added, at room temperature instead
45 min are answered, the μ L of 10 g/L lysines 100 are added, continues to react 15 min, makes mark reaction terminating.
（4）Label NSP-DMAE-NHS-Ab and free NSP-DMAE-NHS passes through Sephadex G-50 posts（1×
25cm）Separation, is that PBS that 6.3, concentration is 0.1mol/L is balanced and eluted chromatographic column with purification buffer pH values.
（5）Protein peak is detected with chromatograph in separation process, the chemiluminescence intensity and 280nm that efflux is measured respectively are inhaled
Shading value.
（6）Shading value height and the big eluent of absorbance are collected, 1%BSA is added（Volume）After dispense stored frozen.
Embodiment 3：The detection of kit
1. Sample pretreatment
（1）The acquisition of milk test sample solution：The μ L of fresh milk 150 are taken in 500 μ L centrifuge tubes, 4 DEG C of 10 min of centrifugation
（3000 r/min）, discard upper-layer fat.The μ L of milk sample 25 after centrifugation are pipetted in clean teat glass, 950 are added
μ L concentration is diluted for 0.02 mol/L phosphate buffer.
（2）Take the g of honey 1 to add in 25 mL centrifuge tubes, add the mol/L hydrochloric acid solutions of 2 mL 0.5, vibrated with oscillator
All dissolve, warmed in 55-75 DEG C of hot water several minutes to honey, add the hydroxide that 5 mL concentration are 0.2 mol/L
Sodium, regulation pH value range is 4.0-6.0, and vibration is mixed.8 mL ethyl acetate are added, are spun upside down after 5 min of vibration, centrifugation
10min（3000 r/min）.Take the mL of upper strata organic solvent 5 with clean glass tube, being dried up in air, 0.5 mL is added dropwise
Concentration is 0.02 mol/L phosphate buffers, is mixed, to be checked.
2. course of reaction
（1）By the μ L of sample to be tested 100, the μ L of coupling magnetic particle suspension 150, the μ L of acridinium ester label 150, sequentially add anti-
Ying Guanzhong, vibration is mixed, 37 DEG C of 15 min of incubation.
（2）Separation, washing 5 times.
（3）Reaction vessel after washing, which is fully vibrated, makes magnetic particle be uniformly dispersed.
（4）100 μ L chemiluminescence exciting liquid B are added after adding 100 μ L chemiluminescence preexciting liquid A, 1 s, it is measured
The content of sulphadiazine and its luminous intensity proportion relation in relative luminous intensity, sample.
Embodiment 4：The performance indications of kit
（1）The sensitivity of kit
20 retests are carried out to zero standard solution, the average value for taking zero standard solution to determine adds 3 times of standard deviation, i.e.,
For the sensitivity of this kit.Sensitivity of this kit to sulphadiazine is 0.030 μ g/L.
（2）The specificity of kit
The competition medicine similar to sulphadiazine structure or function：Sulfamethazine, 5-methoxysulfadiazine, sulfanilamide (SN) quinoline
Dislike beautiful jade.By kit step operation, it is separately added into：Sulphadiazine, sulfamethazine, 5-methoxysulfadiazine, sulfanilamide (SN) quinoline
Beautiful jade is disliked, suppression curve is made, 50% inhibition concentration of each medicine is calculated according to linear equation.Cross reacting rate is antibody to sulphur
The IC of amic metadiazine50With IC of the antibody to sulphadiazine competitor50The ratio between percentage.As a result show：Kit is to sulphadiazine
With higher specificity, pair with sulphadiazine structure or intimate equal no cross reaction of competition medicine.
It is obvious to a person skilled in the art that the invention is not restricted to the details of above-mentioned one exemplary embodiment, Er Qie
In the case of without departing substantially from spirit or essential attributes of the invention, the present invention can be realized in other specific forms.Therefore, no matter
From the point of view of which point, embodiment all should be regarded as exemplary, and be nonrestrictive, the scope of the present invention is by appended power
Profit is required rather than described above is limited, it is intended that all in the implication and scope of the equivalency of claim by falling
Change is included in the present invention.
Moreover, it will be appreciated that although the present specification is described in terms of embodiments, not each embodiment is only wrapped
Containing an independent technical scheme, this narrating mode of specification is only that for clarity, those skilled in the art should
Using specification as an entirety, the technical solutions in the various embodiments may also be suitably combined, forms those skilled in the art
It may be appreciated other embodiment.
Claims (9)
1. the chemiluminescence detection kit and its composition of a kind of sulphadiazine, it is characterised in that including：Coupling has sulphadiazine
The magnetic particle suspension of antibody, the sulphadiazine antigen of acridinium ester label, sulphadiazine serial standards, chemiluminescence preexciting
Liquid A, chemiluminescence exciting liquid B, cleaning fluid.
2. a kind of chemiluminescence detection kit of sulphadiazine according to claim 1, it is characterised in that described change
Luminous marker is acridinium ester, such as NSP-DMAE-NHS, NSP-SA-NHS.
3. a kind of chemiluminescence detection kit of sulphadiazine according to claim 1, it is characterised in that acridinium ester mark
Note is sulphadiazine antigen.
4. a kind of chemiluminescence detection kit of sulphadiazine according to claim 1, it is characterised in that described magnetic
Particulate can directly with sulphadiazine antibody coupling, or magnetic particle and Streptavidin be coupled, while using biotin labeling sulphur
Amic metadiazine antibody.
5. a kind of chemiluminescence detection kit of sulphadiazine according to claim 1, it is characterised in that described change
Luminous preexciting liquid A is learned by H2O2And HNO3 Mixed liquor composition, chemiluminescence exciting liquid B is by Triton X-100 and NaOH
Mixed liquor is constituted.
6. a kind of chemiluminescence detection kit of sulphadiazine according to claim 1, it is characterised in that described school
Quasi- product are, using the Tris-HCl buffer solutions containing 0.5-5.0% BSA and 0.1-0.5% PC300 as matrix, to add sulphadiazine pure
The calibration object solution of the series concentration gradient of product configuration.
7. a kind of chemiluminescence detection kit of sulphadiazine, it is characterised in that including：Coupling has the magnetic of sulphadiazine antigen
Micronised suspensions, acridinium ester label be sulphadiazine antibody, sulphadiazine serial standards, chemiluminescence preexciting liquid A, change
Learn luminous exciting liquid B, cleaning fluid.
8. a kind of chemiluminescence detection kit of sulphadiazine according to claim 7, it is characterised in that acridinium ester mark
Note is sulphadiazine antibody.
9. a kind of chemiluminescence detection kit of sulphadiazine according to claim 7, it is characterised in that described magnetic
Particulate directly can be coupled with sulphadiazine antigen, or magnetic particle and Streptavidin are coupled, while using biotin labeling sulphur
Amic metadiazine antigen.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710726759.1A CN107290525A (en) | 2017-08-24 | 2017-08-24 | A kind of chemiluminescence detection kit of sulphadiazine and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710726759.1A CN107290525A (en) | 2017-08-24 | 2017-08-24 | A kind of chemiluminescence detection kit of sulphadiazine and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107290525A true CN107290525A (en) | 2017-10-24 |
Family
ID=60106652
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710726759.1A Pending CN107290525A (en) | 2017-08-24 | 2017-08-24 | A kind of chemiluminescence detection kit of sulphadiazine and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107290525A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108982841A (en) * | 2018-09-25 | 2018-12-11 | 沭阳康源泰博生物科技有限公司 | A kind of sulfamido Sample pretreatment kit based on immunomagnetic beads |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102079788A (en) * | 2009-11-27 | 2011-06-01 | 北京维德维康生物技术有限公司 | Method for detecting sulfanilamide medicine and special enzyme-linked immunoassay reagent kit thereof |
| CN102565405A (en) * | 2011-08-24 | 2012-07-11 | 苏州长光华医生物试剂有限公司 | Method for immunological detection by combining acridinium ester labeling technology with general magnetic particles |
| CN102928417A (en) * | 2011-08-09 | 2013-02-13 | 北京勤邦生物技术有限公司 | Magnetic particle chemiluminescence kit for detecting sulfanilamide drugs, and applications thereof |
| CN104849469A (en) * | 2015-04-16 | 2015-08-19 | 广州市达瑞生物技术股份有限公司 | Kit for detecting NGAL content and preparation method thereof |
| CN106855572A (en) * | 2016-06-30 | 2017-06-16 | 深圳市亚辉龙生物科技股份有限公司 | A kind of gastrin-releasing peptide precursor chemiluminescence immune detection reagent kit and preparation method thereof |
-
2017
- 2017-08-24 CN CN201710726759.1A patent/CN107290525A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102079788A (en) * | 2009-11-27 | 2011-06-01 | 北京维德维康生物技术有限公司 | Method for detecting sulfanilamide medicine and special enzyme-linked immunoassay reagent kit thereof |
| CN102928417A (en) * | 2011-08-09 | 2013-02-13 | 北京勤邦生物技术有限公司 | Magnetic particle chemiluminescence kit for detecting sulfanilamide drugs, and applications thereof |
| CN102565405A (en) * | 2011-08-24 | 2012-07-11 | 苏州长光华医生物试剂有限公司 | Method for immunological detection by combining acridinium ester labeling technology with general magnetic particles |
| CN104849469A (en) * | 2015-04-16 | 2015-08-19 | 广州市达瑞生物技术股份有限公司 | Kit for detecting NGAL content and preparation method thereof |
| CN106855572A (en) * | 2016-06-30 | 2017-06-16 | 深圳市亚辉龙生物科技股份有限公司 | A kind of gastrin-releasing peptide precursor chemiluminescence immune detection reagent kit and preparation method thereof |
Non-Patent Citations (2)
| Title |
|---|
| 楚金申 等: "直接竞争化学发光酶免疫法检测猪肉中磺胺嘧啶", 《食品科学》 * |
| 王玲 等: "磁性化学发光酶免疫法检测猪肉中的磺胺类药物", 《河北农业大学学报》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108982841A (en) * | 2018-09-25 | 2018-12-11 | 沭阳康源泰博生物科技有限公司 | A kind of sulfamido Sample pretreatment kit based on immunomagnetic beads |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101165491B (en) | Method for detecting double-chain DNA resistant antibody using gold magnetic particle as carrier | |
| CN107831314A (en) | A kind of N middle-ends BGP chemiluminescence detection kit and preparation method thereof | |
| CN107543932A (en) | The magnetic microparticle chemiluminescence detection kit and preparation method of a kind of calcitonin | |
| CN104198721B (en) | The preparation of a kind of silica-based magnetic bead bond of GP73 (GP73) antigen and application | |
| CN101358979B (en) | Chloramphenicol immune detecting system marked by magnetic bead | |
| Arakawa et al. | Chemiluminescence enzyme immunoassay of dehydroepiandrosterone and its sulfate using peroxidase as label | |
| CN109239367A (en) | Measure the method and kit of small molecule compound | |
| CN108196055B (en) | Kit for determining digoxin content by magnetic particle chemiluminescence method and detection method thereof | |
| CN107290526A (en) | A kind of chemiluminescence detection kit of tetracycline and preparation method thereof | |
| CN106661116B (en) | The measuring method of vitamin D epimer | |
| CN108508001A (en) | Chemiluminescence detection kit | |
| CN107796803A (en) | A kind of the magnetic microparticle chemiluminescence detection kit and preparation method of free β human chorionic gonadotrophins | |
| CN107247135A (en) | A kind of chemiluminescence detection kit of sulfamethazine and preparation method thereof | |
| Wang et al. | Directional evolution of TetR protein and development of a fluoroimmunoassay for screening of tetracyclines in egg | |
| CN102608316B (en) | Kit or test strip for detecting quinoxaline compound | |
| CN103018445A (en) | Chemiluminiscence immunoassay quantitative detection kit for saccharide antigen 50 magnetic particles and preparation method thereof | |
| CN107843734A (en) | The magnetic microparticle chemiluminescence detection kit and preparation method of a kind of sex hormone binding globulin | |
| CN102621322B (en) | Kit for detecting 3-methyl-quinoxaline-2-carboxylic acid | |
| CA2636377A1 (en) | Determination of concentration of fk778 by competitive immunoassay | |
| CN107561269A (en) | The magnetic microparticle chemiluminescence detection kit and preparation method of a kind of salbutamol | |
| CN107290525A (en) | A kind of chemiluminescence detection kit of sulphadiazine and preparation method thereof | |
| CN109239368A (en) | Measure the method and kit of progesterone | |
| CN107688016A (en) | A kind of chemiluminescence detection kit of Aflatoxins M1 and preparation method thereof | |
| CN107478827A (en) | A kind of chemiluminescence detection kit of fumonisin and preparation method thereof | |
| Ara et al. | Dot‐Elisa for the rapid detection of gentamicin in milk |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20171024 |