CN107287260A - The production method of low-cost high-quality xanthans - Google Patents
The production method of low-cost high-quality xanthans Download PDFInfo
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Abstract
The present invention provides the production method of low-cost high-quality xanthans, and this method includes：The fermentation medium using dry method corn flour as primary raw material is utilized, is fermented with Xanthomonas campestris, zymotic fluid is made；Xanthans is obtained using zymotic fluid post-processing step；Wherein, counted using the cumulative volume of fermentation medium as 100%, described fermentation medium includes the dry method corn flour that mass volume ratio is 4~7%, and the unit of the mass volume ratio is Kg/L.In addition, the present invention is provided with the fermentation medium of Xanthomonas campestris fermenting and producing xanthan gum, the culture medium includes primary raw material dry method corn flour.The present invention reduces the production cost of xanthans by the replacement of fermentation raw material.
Description
Technical field
The present invention relates to the production method of xanthans production technical field, more particularly to low-cost high-quality xanthans.
Background technology
Xanthans be it is a kind of by Xanthomonas campestris ferment produce Extra-cellular acidic heteroglycan, the polysaccharide high-molecular compound being made up of D-Glucose, D-MANNOSE and D-Glucose aldehydic acid, relative molecular mass is more than 1,000,000.The secondary structure of xanthans be side chain around main chain backbone reverse-winding, maintain to form bar-shaped double-spiral structure by hydrogen bond, unique structure forms the extremely strong chemical stability of xanthans.
Xanthans is the biogum that current best performance is got over.It has the general performance of long chain macromolecule, while it contains more functional group than general macromolecule, special performance can be shown under given conditions.Xanthans is lurid powder under normal circumstances, because it has excellent thickening property, suspension, emulsibility and water solubility, and with good heat, ph stability, so be widely used in various food, cosmetics, medicine, oil recovery, printing and dyeing, papermaking, weaving, the field such as ceramics and spices.But, the cost of microbial fermentation production xanthans remains high, on the one hand it is due to the cost accounting weight of culture medium, in the prior art, the carbon source produced in xanthans culture medium is more based on cornstarch, glucose, sucrose, nitrogen source is then more based on bean powder, soyabean protein powder, and these materials value is higher, causes the production cost of xanthans higher；On the other hand, the production cost height of xanthans is due to that glue rate is low causes for the production of xanthans.Therefore, how to reduce production cost turns into the key technology that xanthans is promoted.For many years, people are directed to reducing always the cost of culture medium and/or improve the research that xanthans produces glue rate.CN101363037A discloses a kind of xanthans culture medium prepared with starch wastewater and active medical stone granule, although the culture medium can reduce culture medium cost to a certain extent, its preparation process is very cumbersome, and the popularization and application of xanthans are not utilized.CN1539987A discloses the method fermented using beet molasses culture medium.CN104388493A discloses a kind of method for improving xanthans production conversion ratio, and the case can improve xanthans production glue rate to about 3.9g/100mL.Although prior art can partly reduce the production cost of xanthans, limitation, therefore, how further to solve xanthans production cost height is one of this area urgent problem to be solved.
On the other hand, in recent years, with the continuous expansion of the market demand, xanthans production scale constantly increases, up to the present, and domestic production amount is more than 100,000 tons/year.The expansion of yield causes product market competition fierce, price keeps falling, manufacturing enterprise's profit is more and more lower, the edge of cost is even arrived, simultaneously, the disorderly competition of the market price causes manufacturing enterprise to have no time to attend to product quality, so as to further limit expansion of the xanthans in more high-end market segment.
The content of the invention
Regarding to the issue above, it is a primary object of the present invention to provide a kind of method for producing xanthans as carbon source in fermentation medium and nitrogen source by the use of dry method corn flour, the purpose for reducing cost, economizing on resources is reached by the comprehensive utilization to primary agricultural and sideline processed goods dry method corn flour.
To achieve the above object, the present invention provides xanthan gel, and it includes：
Step (a)：Using the fermentation medium that dry method corn flour is primary raw material, fermented with Xanthomonas campestris, zymotic fluid is made；
Step (b)：Xanthans is obtained using zymotic fluid post-processing step；
Wherein, counted using the cumulative volume of fermentation medium as 100%, described fermentation medium includes the dry method corn flour that mass volume ratio is 4%~7%, and the unit of the mass volume ratio is Kg/L.
The present invention is using dry method corn flour as the primary carbon source and nitrogen source in fermentation medium, and the traditional carbon source (such as glucose, sucrose, starch) and nitrogen source (such as bean powder, soyabean protein powder) substituted in xanthan gum fermentation production carries out microbial fermentation.Due to containing starch, protein in dry method corn flour, therefore it can play a part of carbon source and nitrogen source in substitutive medium simultaneously, again because dry method corn flour is the primary agricultural byproducts Jing Guo dry method preliminary working, its market price is relatively low, this greatly reduces carbon source in culture medium raw material, the cost of nitrogen source, therefore, use the present invention not only can provide a kind of new production ways for the production of xanthans, can also reduce production cost.
In the inventive method embodiment, preferably, counted using the cumulative volume of fermentation medium as 100%, described fermentation medium include the dry method corn flour that mass volume ratio is 4%~7%, mass volume ratio be 0.3%~0.4% precipitated calcium carbonate, mass volume ratio be 0.02%~0.04% yeast extract, mass volume ratio be MnSO that 0.05%~0.06% peptone, mass volume ratio are 0.01%~0.02%4, mass volume ratio be 0.01%~0.02% MgSO4, the water that mass volume ratio is 0.03%~0.04% defoamer and surplus, the unit of the mass volume ratio is Kg/L.It is highly preferred that the defoamer is polyethers (such as PPG) defoamer and/or organic silicon (such as dimethyl silicone polymer) defoamer.
Material used of the invention is commercially available, it is preferable that the Xanthomonas campestris is sarson Xanthomonas campestris, it is highly preferred that the strain that it is CGMCC1.1654 purchased from CGMCC deposit number that the Xanthomonas campestris, which is,.
Dry method corn flour of the present invention is：Corn is soaked with a small amount of water, is directly peeled by dry method, is taken off embryo, obtained corn embryosperm (cornstarch and zein) directly carries out crushing and formed.Dry method corn flour of the present invention requires no the wet method decortication of sulfurous acid soak, de- embryo, separating corn albumen.In a specific embodiment, the dry method corn flour can be prepared via a method which to obtain：
Use corn for raw material, its total moisture content is reached 18wt%~19wt%, after placement, peeled by dry method, de- embryo obtains endosperm, endosperm, which pulverizes and sieves, to be obtained.
For example, corn is used for raw material, spray a small amount of water, its total moisture content is reached 18wt%~19wt%, (commercially available corn is for the ease of preservation, moisture is general in 13~15wt%), place 2~4 hours, maize peel, endosperm fully absorb certain moisture and with after sufficiently ductile, peeled by dry method, take off embryo, endosperm, which pulverizes and sieves, to be obtained.
The present invention is determined through experimentation the optimal conditions of zymotechnique, its be ferment 0~12 when the stage in, control fermentation temperature be 27.0~30.0 DEG C, control pH to be 7.1~7.4, control air quantity to be 0.20~0.22vvm (it is 0.06~0.07MPa that corresponding tank, which is pressed), mixing speed is 100~120r/min；Ferment 12~24 when the stage in, control fermentation temperature be 27.0~30.0 DEG C, control pH to be 6.9~7.2, control air quantity to be 0.24~0.26vvm (it is 0.05~0.06MPa that corresponding tank, which is pressed), mixing speed is 120~150r/min；Ferment 24~36 when the stage in, control fermentation temperature be 28.0~31.0 DEG C, control pH to be 6.8~7.1, control air quantity to be 0.28~0.30vvm (it is 0.04~0.05MPa that corresponding tank, which is pressed), mixing speed is 150~180r/min；Ferment 36~48 when the stage in, control fermentation temperature be 29.0~32.0 DEG C, control pH to be 6.8~7.0, control air quantity to be 0.32~0.34vvm (it is 0.03~0.04MPa that corresponding tank, which is pressed), mixing speed is 180~200r/min；In fermentation 48~60 in the stage, to control fermentation temperature be 30.0~33.0 DEG C, control pH to be 6.8~6.9, control air quantity to be 0.36~0.38vvm (corresponding tank pressure be 0.02~0.03MPa), mixing speed be 200~230r/min, and stopping is fermented when detection residual sugar is 0；The unit of the vvm is m3/m3/min.Fermented by such as table 1 below：
Table 1
In the inventive method embodiment, step (a) may also include：During the fermentation, stream plus cotton dregs hydrolyzate.During the fermentation, stream plus cotton dregs hydrolyzate, supplemented with alpha-amido nitrogen source, can promote microbial metabolism, total yield glue rate is reached more than 5.2g/100mL, and this is not than using lifting 0.5g/100mL or so during this technique, improving production glue rate up to more than 11%.
Cotton dregs are the byproduct after cottonseed extracts oil, and market value is relatively low, can effectively utilize the byproduct by being prepared into cotton dregs hydrolyzate, produce economic value.Preferably, cotton dregs hydrolyzate of the present invention is cotton dregs hydrolyzate made from alkali hydrolysis method.I.e. heating a period of time hydrolyzes cotton dregs in the basic conditions, and in cotton dregs 85% protein can be hydrolyzed into the macromolecular amino acid rich in α-amino nitrogen by alkali hydrolysis method；It is highly preferred that cotton dregs hydrolyzate is made as follows made from the alkali hydrolysis method：Cotton dregs are pulverized and sieved, and 3%~6% mass concentration is formulated as with water, and regulation pH is 11.0~11.5, is adjusted for example with mass fraction for 2%~10% NaOH solution, 2~4h is hydrolyzed under the conditions of 80~85 DEG C, liquid phase is isolated and obtains the cotton dregs hydrolyzate.Cotton dregs powder raw material is commercially available, for example, the commercially available coarse cotton dregs of rice can be crushed to 40 sieves basic hydrolysis material is made.Alpha-amido nitrogen source can not only be supplemented using cotton dregs hydrolyzate made from alkali hydrolysis method, because itself is alkalescence, can also be used to adjust the pH in fermentation process.As described above, the present invention can control the pH in fermentation process using cotton dregs hydrolyzate made from alkali hydrolysis method, fermentation pH not only be can adjust by flowing cotton dregs hydrolyzate made from alkaline hydrolysis method, also supplemented with alpha-amido nitrogen source, microbial metabolism is promoted, the production glue rate of xanthans is improved.Preferably, cotton dregs hydrolyzate made from alkaline hydrolysis method is flowed in the following way：Add when fermentation to 10~12h starts stream, the cumulative volume of cotton dregs hydrolyzate made from the alkali hydrolysis method that stream adds is the 8%~10% of the constant volume of fermentation medium, 4~6 streams are divided to add, stream adds to pH7.2~7.3 first, stream plus time started reach that each stage control is offline for fermentation pH every time later, and stream adds to fermentation pH and reaches that the stage control is reached the standard grade.For example in fermentation 24~36 in the stage, when pH reaches 6.8, start cotton dregs hydrolyzate made from stream alkaline hydrolysis method, stop stream when pH reaches 7.1 and add the cotton dregs hydrolyzate.
In the inventive method embodiment, the zymotic fluid post-processing step includes decolourizing and/or drying steps；Preferably, it is 5.5~6.0, decolouring, extraction and drying steps that the zymotic fluid post-processing step includes regulation zymotic fluid pH successively.
Preferably, it is of the present invention to decolourize to be to decolourize to zymotic fluid with hydrogen peroxide and sulphite.The hydrogen peroxide can be the hydrogen peroxide that mass fraction is 30%；The sulphite includes anhydrous sodium sulfite.
It is further preferred that it is 5.5~6.0 that the decolorization process, which is the zymotic fluid salt acid for adjusting pH that will be obtained in step (a), the 30%H that volume fraction is 0.3%~0.5% is added2O2Hydrogen peroxide, stir after 30~40min, add the anhydrous sodium sulfite that quality volume fraction is 0.15%~0.20%, stir 30~40min, the unit of the quality volume fraction is g/mL, and the volume fraction and the quality volume fraction are relative to the volume of the zymotic fluid.
The present invention is decolourized using hydrogen peroxide and anhydrous sodium sulfite to zymotic fluid, and the whiteness of product xanthans is significantly improved, and improves the quality of product.
Preferably, drying of the present invention is to use the continuous drying technique of vacuum belt low temperature.It is highly preferred that the continuous drying technique of vacuum belt low temperature is through decolourizing, extracting in zymotic fluid, after isolated xanthans wet product, it is placed in vacuum belt continuous low temperature drying machine, sequentially passes through first, second and third dry section and cooling zone.It is further preferred that being 75 DEG C~80 DEG C by the temperature of the first dry section, the time is 5min~10min；It it is 65 DEG C~75 DEG C by the temperature of the second dry section, the time is 10min~15min；It it is 55 DEG C~65 DEG C by the temperature of the 3rd dry section, the time is 10min~15min；Cooling zone temperature is 20 DEG C~40 DEG C, and the time is 10min~15min；Vacuum degree control is -0.08MPa to -0.09MPa in the drying machine, and the crawler belt speed of service is 15~20cm/min, and charging rate is 10~15Kg/h/m2.After the size-reduced sieving of xanthan gum product after drying, powdered xanthans finished product is obtained.
Prior art xanthans, which is dried, uses pipe bundle drier, because pipe bundle drier is not totally enclosed type operation, vacuum is only capable of control below -0.02MPa, drying temperature is high and the dry materials residence time is long, and use the traditional tube bank drying process of vacuum belt continuous low temperature drying technical substitution of the present invention, the drying time of pipe bundle drier from 70min or so can be shorten to 40min or so, temperature is reduced to 80 DEG C of highest from 120 DEG C or so, so as to save the energy, vacuum belt continuous low temperature drying machine can be realized totally-enclosed simultaneously, continuous and automatic is operated, equipment is easy to cleaning compared to pipe bundle drier, drying efficiency is obviously improved.
Preferably, step (a) of the present invention includes：During the fermentation, cotton dregs hydrolyzate as described above is added by as above fed-batch mode stream；Preferably, the cotton dregs hydrolyzate is cotton dregs hydrolyzate made from alkali hydrolysis method as described above；Fermentation process is as described above；
And zymotic fluid post-processing step is as described above described in step (b).According to the technique, calculated with the scale that regular fermentation process produces 10000 tons of xanthans per year, the direct economic benefit produced every year calculates as shown in table 2：
Table 2
From Table 2, it can be seen that using as above technique, 15,000,000 yuan of economic benefits can be produced by producing 10000 tons of xanthans production scales per year.
The present invention is updated by production technology and technique, replacement especially to producing supplementary material, application to new technology, new technology is constantly studied, primary processed farm and sideline products (dry method corn flour) are applied to the higher supplementary material of Replacement value during xanthans is produced, also the technique such as fermentation, extraction, dry is innovated, this method rational technology, it is easily operated automatically, xanthans production cost can not only be reduced, xanthan gum product quality can also be lifted, products application scope has been expanded, economic benefit and social value is created.
On the other hand, the present invention is provided with the fermentation medium of Xanthomonas campestris fermenting and producing xanthan gum, and the culture medium includes primary raw material dry method corn flour.Preferably, counted using the cumulative volume of fermentation medium as 100%, described fermentation medium may include the dry method corn flour that mass volume ratio is 4%~7%, and the unit of the mass volume ratio is Kg/L.It is highly preferred that also include in the culture medium mass volume ratio be 0.3%~0.4% precipitated calcium carbonate, mass volume ratio be 0.02%~0.04% yeast extract, mass volume ratio be MnSO that 0.05%~0.06% peptone, mass volume ratio are 0.01%~0.02%4, mass volume ratio be 0.01%~0.02% MgSO4, the water that mass volume ratio is 0.03%~0.04% defoamer and surplus.Preferably, the defoamer is polyethers (such as PPG) defoamer and/or organic silicon (such as dimethyl silicone polymer) defoamer.
It is further preferred that the dry method corn flour can be prepared via a method which to obtain：
Use corn for raw material, its total moisture content is reached 18wt.%~19wt.%, after placement, peeled by dry method, de- embryo obtains endosperm, endosperm, which pulverizes and sieves, to be obtained.
As described above, can effectively reduce the cost in xanthan gel using the culture medium.
Another further aspect, the present invention provides application of the fermentation medium in Xanthomonas campestris fermenting and producing xanthan gum.
The beneficial effects of the present invention are：By the replacement of fermentation raw material, xanthans cost of material is reduced；By using stream plus the zymotechnique of cotton dregs hydrolyzate, fermentation production glue rate is than regular fermentation process lifting 0.5g/100ml or so；Drying efficiency is improved by the application of vacuum belt continuous low temperature drying technology；Additionally by the decolouring to zymotic fluid, xanthan gum product is set to be become colorless substantially from the yellow in traditional handicraft, quality is obviously improved, CSAT lifting, so as to expand the scope of market application.
Embodiment
In order to which technical characteristic, purpose and beneficial effect to the present invention are more clearly understood from, technical scheme is carried out in conjunction with specific embodiment described further below, it should be understood that these examples are only illustrative of the invention and is not intended to limit the scope of the invention.
Unless otherwise instructed, the dry method corn flour in following examples is prepared in accordance with the following steps：By corn is with a small amount of water spray and stirs, total moisture content is reached 18wt.%~19wt.%, place 2h, maize peel, plumule is allowed to absorb after certain moisture with sufficiently ductile, then embryo is taken off by peeling machine decortication, germ separator, filtered out after maize peel and plumule, endosperm crushed 60 mesh sieves.
Unless otherwise instructed, the strain that it is CGMCC1.1654 purchased from the deposit number of China General Microbiological culture presevation administrative center (China General Microbiological Culture Collection Center, CGMCC) that xanthomonas campestris seed used, which is, in following examples.
Embodiment 1
The present embodiment provides a kind of production method of low-cost high-quality xanthans, and this method comprises the following steps：
(1) 250L fermentations ullage disappears, and temperature sets 129~130 DEG C, tank pressure 0.19~0.20MPa, time 30min；
(2) 25L streams plus ullage disappear, 129~130 DEG C of temperature, tank pressure 0.18~0.20MPa, time 30min；
(3) culture medium is added in 250L fermentation tanks, the culture medium is：Dry method corn flour 10.8Kg, precipitated calcium carbonate 576g, yeast extract 54g, peptone 108g, MnSO418g, MgSO418g, PPG defoamer 54g, add 100L water and are sufficiently stirred for after 30min, add pure water and determine volume 180L；
(4) 900g cotton dregs are added in 25L hydrolytic decomposition pots, add about 5L water and be sufficiently stirred for 30min, to cotton dregs even suspension in water after, the stirring that adds water is set to 20L, it is 11.3 to add the NaOH aqueous solution regulation pH that mass fraction is 10%, 83 DEG C of hydrolysis 4h are warming up to, after being separated with centrifuge, liquid phase is transferred to 25L streams plus tank；
(5) 25L stream plus tank disappear in fact, 115~116 DEG C of temperature, tank pressure 0.10~0.12MPa, time 15min, ventilation keep tank pressure 0.06MPa, be cooled to 29 DEG C it is standby；
(6) 250L fermentation tanks disappear in fact, 124~125 DEG C of temperature, tank pressure 0.13~0.15MPa, time 30min, ventilation keep tank pressure 0.03MPa, be cooled to 28.5 DEG C it is standby；
(7) the xanthomonas campestris seed asepsis of 2L Shaking cultures is accessed in 250L fermentation tanks, fermented, ferment control parameter is as shown in the table：
(8) during fermentation 11h, stream plus cotton dregs hydrolyzate are started, stream plus volume about 5L, 3h or so stream are added, and now pH reaches 7.3；When the pH that ferments is begun to decline to 6.9 for the second time, stream plus 5L, 5h or so stream are added, and now pH reaches 7.2；When pH third times of fermenting are begun to decline to 6.8, stream plus 5L, 5h or so stream are added, and now pH reaches 7.1；When the pH that ferments is begun to decline to 6.8 for the 4th time, stream plus 5L, 5h or so stream are added, and now pH reaches 7.0；
(9) fermentation to 45h when start detection production glue, fermentation 58h or so, when detection production glue is that 5.2g/100mL, residual sugar are 0, stops fermentation, zymotic fluid be transferred to extractor；
(10) it is 5.7 to adjust extract solution pH with hydrochloric acid, adds 600mL hydrogen peroxide (30%H2O2), 35min is stirred, 300g anhydrous sodium sulfites (Na is then added2SO3), stir 35min；
(11) it is 6.5 to adjust extract solution pH with liquid caustic soda, adds 85% alcohol, and stirring and adjusting to alcoholic strength 75% or so keeps 20min, starts centrifugation；
(12) product after centrifugation is placed in vacuum belt continuous low temperature drying machine, sequentially passes through first, second and third dry section and cooling zone.It is set as 75 DEG C by the temperature of the first dry section, the time is 5min；It it is 65 DEG C by the temperature of the second dry section, the time is 10min；It it is 55 DEG C by the temperature of the 3rd dry section, the time is 10min；Cooling zone temperature is 25 DEG C, and the time is 10min.Vacuum degree control is -0.08MPa in drying machine, and the crawler belt speed of service is 15cm/min, and charging rate is 10Kg/h/m2.The dried size-reduced mesh sieve of mistake 60 of product, obtains powdered finished product about 10Kg, product whiteness is obviously improved, and is detected as 75.0%.
Embodiment 2
The present embodiment provides a kind of production method of low-cost high-quality xanthans, and this method comprises the following steps：
(1)2.5m3Fermentation ullage disappears, and temperature sets 128~129 DEG C, tank pressure 0.18~0.19MPa, time 30min；
(2) 250L streams plus ullage disappear, 128~129 DEG C of temperature, tank pressure 0.18~0.19MPa, time 30min；
(3) 250L seeds ullage disappears, 128~129 DEG C of temperature, tank pressure 0.18~0.19MPa, time 30min, dispensing after cooling, white sugar 2.0Kg, peptone 130g, bean powder 1.05Kg, yeast extract 250g, sodium chloride 160g, precipitated calcium carbonate 160g, defoamer 50g, K2HPO430g, MgSO430g, plus pure water determine volume for 200L；
(4) 9Kg cotton dregs are added in 250L hydrolytic decomposition pots, add about 50L water and be sufficiently stirred for 30min, to cotton dregs even suspension in water after, the stirring that adds water is set to 200L, again plus mass fraction be 10% the NaOH aqueous solution regulation pH be 11.4,84 DEG C of hydrolysis 4h are warming up to, after being separated with centrifuge, liquid phase is transferred to 250L streams plus tank；
(5)2.5m3Fermentation tank cools dispensing, and culture medium is：Dry method corn flour 108Kg, precipitated calcium carbonate 5.76Kg, yeast extract 540g, peptone 1.08Kg, MnSO4180g, MgSO4180g, PPG defoamer 540g, add 1m3Water is sufficiently stirred for after 30min, is added pure water and is determined volume 1.8m3；
(6) 250L seeding tanks disappear in fact, 124~125 DEG C of temperature, and tank pressure 0.13~0.15MPa, time 30min, ventilation keep tank pressure 0.03MPa, are cooled to after 28.5 DEG C, and sterile access 4L shaking flask xanthomonas campestrises seed carries out seed and expands culture；
(7) 250L seeding tanks ferment, and control 28.5 DEG C of temperature, ventilation 2.4m3/ h, tank pressure 0.03MPa, speed of agitator 110r/min, cultivates 12h；
(8)2.5m3Fermentation tank disappears in fact, 124~125 DEG C of temperature, tank pressure 0.13~0.15MPa, time 25min, ventilation keep tank pressure 0.03MPa, be cooled to 28.7 DEG C it is standby；
(9) 250L stream plus tank disappear in fact, 116~117 DEG C of temperature, tank pressure 0.10~0.12MPa, time 15min, ventilation keep tank pressure 0.06MPa, be cooled to 28.5 DEG C it is standby；
(10) seed during 250L seeding tanks are fermented accesses 2.5m3Fermentation tank is fermented, and process control parameter is as shown in the table：
(11) during fermentation 11h, stream plus cotton dregs hydrolyzate are started, stream plus volume about 50L, 3h or so stream are added, and now pH reaches 7.3；When the pH that ferments is begun to decline to 6.9 for the second time, stream plus 50L, 4h or so stream are added, and now pH reaches 7.2；When pH third times of fermenting are begun to decline to 6.8, stream plus 50L, 4h or so stream are added, and now pH reaches 7.1；When the pH that ferments is begun to decline to 6.8 for the 4th time, stream plus 50L, 4h or so stream are added, and now pH reaches 7.0；
(12) fermentation to 45h when start detection production glue, fermentation 56h or so, when detection production glue is that 5.25g/100mL, residual sugar are 0, stops fermentation, zymotic fluid be transferred to extractor；
(13) it is 5.7 to adjust extract solution pH with hydrochloric acid, adds 6L hydrogen peroxide (30%H2O2), 35min is stirred, 3Kg anhydrous sodium sulfites (Na is then added2SO3), stir 35min；
(14) it is 6.5 to adjust extract solution pH with liquid caustic soda, adds 85% alcohol, and stirring and adjusting to alcoholic strength 75% or so keeps 20min, starts centrifugation；
(15) product after centrifugation is placed in vacuum belt continuous low temperature drying machine, sequentially passes through first, second and third dry section and cooling zone.It is set as 75 DEG C by the temperature of the first dry section, the time is 7min；It it is 65 DEG C by the temperature of the second dry section, the time is 12min；It it is 55 DEG C by the temperature of the 3rd dry section, the time is 13min；Cooling zone temperature is 25 DEG C, and the time is 12min.Vacuum degree control is -0.085MPa in drying machine, and the crawler belt speed of service is 15cm/min, and charging rate is 10Kg/h/m2.The dried size-reduced mesh sieve of mistake 60 of product, obtains powdered finished product about 105Kg, product whiteness is obviously improved, and is detected as 75.4%.
Embodiment 3
The present embodiment provides a kind of production method of low-cost high-quality xanthans, and this method comprises the following steps：
(1)25m3Fermentation ullage disappears, and temperature sets 128~129 DEG C, tank pressure 0.18~0.19MPa, time 30min；
(2)2.5m3Stream plus ullage disappear, 128~129 DEG C of temperature, tank pressure 0.18~0.19MPa, time 30min；
(3) 250L first order seeds ullage disappears, 128~129 DEG C of temperature, tank pressure 0.18~0.19MPa, time 30min, dispensing after cooling, white sugar 2.0Kg, peptone 130g, bean powder 1.05Kg, yeast extract 250g, sodium chloride 160g, precipitated calcium carbonate 160g, defoamer 50g, K2HPO430g, MgSO430g, plus pure water determine volume for 200L；
(4)2.5m3Secondary seed ullage disappears, 128~129 DEG C of temperature, tank pressure 0.18~0.19MPa, time 30min, dispensing after cooling, the dispensing includes white sugar 18Kg, peptone 1.2Kg, bean powder 10Kg, yeast extract 2.4Kg, sodium chloride 1.5Kg, precipitated calcium carbonate 1.5Kg, defoamer 450g, K2HPO4250g, MgSO4250g, plus pure water determine volume for 1.8m3；
(5)2.5m3In hydrolytic decomposition pot add 90Kg cotton dregs, add about 500L water be sufficiently stirred for 30min, to cotton dregs even suspension in water after, the stirring that adds water is set to 2m3, then add the NaOH solution liquid regulation pH that mass fraction is 10% to be 11.4,84 DEG C of hydrolysis 4h are warming up to, after being separated with centrifuge, liquid phase is transferred to 2.5m3Stream plus tank；
(6)25m3Fermentation tank cools dispensing, and culture medium is：Dry method corn flour 1.08t, precipitated calcium carbonate 57.6Kg, yeast extract 5.4Kg, peptone 10.8Kg, MnSO41.8Kg, MgSO41.8Kg, PPG defoamer 5.4Kg, add 10m3Water is sufficiently stirred for after 30min, is added pure water and is determined volume 18m3；
(7) 250L first class seed pots disappear in fact, 124~125 DEG C of temperature, and tank pressure 0.13~0.15MPa, time 25min, ventilation keep tank pressure 0.03MPa, are cooled to after 28.5 DEG C, and sterile access 4L shake-flask seeds carry out first order seed and expand culture；
(8)2.5m3Secondary seed tank disappears in fact, 124~125 DEG C of temperature, and tank pressure 0.13~0.15MPa, time 30min, ventilation keep tank pressure 0.03MPa, are cooled to 28.5 DEG C；
(9)25m3Fermentation tank disappears in fact, 124~125 DEG C of temperature, and tank pressure 0.13~0.15MPa, time 30min, ventilation keep tank pressure 0.03MPa, are cooled to 28.6 DEG C；
(10)2.5m3Stream plus tank disappear in fact, 117~118 DEG C of temperature, and tank pressure 0.11~0.12MPa, time 15min, ventilation keep tank pressure 0.06MPa, are cooled to 28.5 DEG C；
(11) 250L first class seed pots ferment, and control 28.5 DEG C of temperature, ventilation 2.4m3/ h, tank presses 0.03MPa, speed of agitator 110r/min, cultivates 16h, microscopy strain quantity is enough, grow up full rear immigration 2.5m3Culture is enlarged in secondary seed tank；
(12)2.5m3Secondary seed expands culture, controls 28.5 DEG C of temperature, ventilation 23m3/ h, tank pressure 0.03MPa, speed of agitator 120r/min, cultivates after 8h, moves into 25m3Fermentation tank is fermented；
(13)25m3Fermentation tank is fermented, and process control parameter is as shown in the table：
(14) during fermentation 11h, stream plus cotton dregs hydrolyzate are started, stream plus volume about 500L, 3h or so stream are added, and now pH reaches 7.3；When the pH that ferments is begun to decline to 6.9 for the second time, stream plus 500L, 5h or so stream are added, and now pH reaches 7.2；When pH third times of fermenting are begun to decline to 6.8, stream plus 500L, 5h or so stream are added, and now pH reaches 7.1；When the pH that ferments is begun to decline to 6.8 for the 4th time, stream plus 500L, 4h or so stream are added, and now pH reaches 7.0；
(15) fermentation to 45h when start detection production glue, fermentation 60h or so, when detection production glue is that 5.32g/100mL, residual sugar are 0, stops fermentation, zymotic fluid be transferred to extractor；
(16) it is 5.7 to adjust extract solution pH with hydrochloric acid, adds 62L hydrogen peroxide (30%H2O2), 35min is stirred, 33Kg anhydrous sodium sulfites (Na is then added2SO3), stir 35min.
(17) it is 6.5 to adjust extract solution pH with liquid caustic soda, adds 85% alcohol, and stirring and adjusting to alcoholic strength 75% or so keeps 20min, starts centrifugation.
(18) product after centrifugation is placed in vacuum belt continuous low temperature drying machine, sequentially passes through first, second and third dry section and cooling zone.It is set as 75 DEG C by the temperature of the first dry section, the time is 8min；It it is 65 DEG C by the temperature of the second dry section, the time is 13min；It it is 55 DEG C by the temperature of the 3rd dry section, the time is 14min；Cooling zone temperature is 25 DEG C, and the time is 12min.Vacuum degree control is -0.085MPa in drying machine, and the crawler belt speed of service is 15cm/min, and charging rate is 12kg/h/m2.The dried size-reduced mesh sieve of mistake 60 of product, obtains powdered finished product about 1.06t, product whiteness is obviously improved, and is detected as 75.6%.
A kind of production method of low-cost high-quality xanthans provided by the present invention is described in detail above, specific case used herein is set forth to the key step and embodiment of the present invention, and above-described embodiment is only to aid in understanding the method and central principle of the present invention.For those skilled in the art, according to the central principle of the present invention, each condition and parameter can as needed be changed in specific implementation, in summary, this specification should not be construed as limiting the invention.
Claims (10)
1. the production method of xanthans, it includes：
Step (a)：The fermentation medium using dry method corn flour as primary raw material is utilized, is fermented with Xanthomonas campestris,
Zymotic fluid is made；
Step (b)：Xanthans is obtained using zymotic fluid post-processing step；
Wherein, counted using the cumulative volume of fermentation medium as 100%, described fermentation medium is including mass volume ratio
4%~7% dry method corn flour, the unit of the mass volume ratio is Kg/L.
2. according to the method described in claim 1, wherein, counted using the cumulative volume of fermentation medium as 100%, it is described
Fermentation medium include mass volume ratio be 4%~7% the dry method corn flour, mass volume ratio be
Yeast extract that 0.3%~0.4% precipitated calcium carbonate, mass volume ratio are 0.02%~0.04%, mass volume ratio are
0.05%~0.06% peptone, mass volume ratio are 0.01%~0.02% MnSO4, mass volume ratio be
0.01%~0.02% MgSO4, the water that mass volume ratio is 0.03%~0.04% defoamer and surplus, the quality
The unit of volume ratio is Kg/L；Preferably, the defoamer is polyethers defoamer and/or organic silicon defoamer.
3. according to the method described in claim 1, wherein, the dry method corn flour is prepared via a method which to obtain：
Use corn for raw material, its total moisture content is reached 18~19wt%, after placement, peeled by dry method, take off embryo
Endosperm is obtained, endosperm, which pulverizes and sieves, to be obtained.
4. according to the method described in claim 1, wherein, the fermentation process of step (a) is：In fermentation 0~12
When the stage in, to control fermentation temperature be 27.0~30.0 DEG C, controls pH to be 7.1~7.4, control air quantity to be 0.20~0.22
Vvm, mixing speed are 100~120r/min；In fermentation 12~24 in the stage, it is 27.0~30.0 to control fermentation temperature
DEG C, control pH to be 6.9~7.2, control air quantity to be 0.24~0.26vvm, mixing speed is 120~150r/min；
During fermentation 24~36 in the stage, control fermentation temperature be 28.0~31.0 DEG C, controls pH to be 6.8~7.1, control air quantity
It is 150~180r/min for 0.28~0.30vvm, mixing speed；In fermentation 36~48 in the stage, control fermentation temperature
Degree is 29.0~32.0 DEG C, controls pH to be 6.8~7.0, control air quantity to be 0.32~0.34vvm, mixing speed is 180~200
r/min；Ferment 48~60 when the stage in, to control fermentation temperature be 30.0~33.0 DEG C, control pH to be 6.8~6.9,
It is that 0.36~0.38vvm, mixing speed are 200~230r/min to control air quantity, and detection residual sugar stops fermentation when being 0；
The unit of the vvm is m3/m3/min。
5. the method according to claim 1 or 4, wherein, step (a) also includes：During the fermentation,
Stream plus cotton dregs hydrolyzate；Preferably, the cotton dregs hydrolyzate is cotton dregs hydrolyzate made from alkali hydrolysis method；It is highly preferred that
Cotton dregs hydrolyzate is made as follows made from the alkali hydrolysis method：Cotton dregs are pulverized and sieved, and 3%~6 is formulated as with water
% mass concentration, regulation pH is 11.0~11.5, is carried out for example with mass fraction for 2~10% NaOH solution
Regulation, 2~4h is hydrolyzed under the conditions of 80~85 DEG C, liquid phase is isolated and obtains the cotton dregs hydrolyzate.
6. method according to claim 5, wherein, cotton dregs made from alkaline hydrolysis method are flowed in the following way
Hydrolyzate：Add when fermentation to 10~12h starts stream, the cumulative volume of cotton dregs hydrolyzate is made from the alkali hydrolysis method that stream adds
The 8%~12% of the constant volume of fermentation medium, point 4~6 streams are added, and stream adds to pH7.2~7.3 first, after
Stream plus time started reach that each stage control is offline for fermentation pH every time, and stream adds to fermentation pH and reaches that the stage is controlled
System is reached the standard grade.
7. according to the method described in claim 1, wherein, the zymotic fluid post-processing step includes decolourizing and/or dry
Dry step；
Preferably, described decolouring is that zymotic fluid is decolourized with hydrogen peroxide and sulphite；It is further preferred that
The decolorization process is that the zymotic fluid salt acid for adjusting pH that will be obtained in step (a) is 5.5~6.0, adds volume integral
Number is 0.3%~0.5% 30%H2O2Hydrogen peroxide, stirring 30~40min after, add quality volume fraction be
0.15%~0.20% anhydrous sodium sulfite, stirs 30~40min, the unit of the quality volume fraction is g/mL, should
Volume fraction and the quality volume fraction are relative to the volume of the zymotic fluid.
8. method according to claim 7, wherein, the drying is continuous drying using vacuum belt low temperature
Technique；
Preferably, the continuous drying technique of vacuum belt low temperature is through decolourizing, extracting, isolated xanthan in zymotic fluid
After glue wet product, it is placed in vacuum belt continuous low temperature drying machine, sequentially passes through first, second and third dry section and cold
But area；It is further preferred that being 75 DEG C~80 DEG C by the temperature of the first dry section, the time is 5min~10min；
It it is 65 DEG C~75 DEG C by the temperature of the second dry section, the time is 10min~15min；By the temperature of the 3rd dry section
Spend for 55 DEG C~65 DEG C, the time is 10min~15min；Cooling zone temperature is 20 DEG C~40 DEG C, and the time is 10min~15
min；Vacuum degree control is -0.08MPa to -0.09MPa in the drying machine, and the crawler belt speed of service is 15~20
Cm/min, charging rate is 10~15Kg/h/m2。
9. with the fermentation medium of Xanthomonas campestris fermenting and producing xanthan gum, the culture medium includes primary raw material dry method corn
Powder；Preferably, counted using the cumulative volume of fermentation medium as 100%, described fermentation medium includes mass volume ratio
For 4%~7% dry method corn flour, the unit of the mass volume ratio is Kg/L；It is highly preferred that the culture
It is the yeast that 0.3%~0.4% precipitated calcium carbonate, mass volume ratio are 0.02%~0.04% also to include mass volume ratio in base
Cream, mass volume ratio are the MnSO that 0.05%~0.06% peptone, mass volume ratio are 0.01%~0.02%4, matter
Measure the MgSO that volume ratio is 0.01%~0.02%4And mass volume ratio is 0.03%~0.04% defoamer and surplus
Water；Preferably, the defoamer is polyethers defoamer and/or organic silicon defoamer；
It is further preferred that the dry method corn flour is prepared via a method which to obtain：
Use corn for raw material, its total moisture content is reached 18wt%~19wt%, after placement, peeled by dry method,
De- embryo obtains endosperm, and endosperm, which pulverizes and sieves, to be obtained.
10. application of the fermentation medium described in claim 9 in Xanthomonas campestris fermenting and producing xanthan gum.
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| CN111187796A (en) * | 2019-01-28 | 2020-05-22 | 健隆生物科技股份有限公司 | Xanthan gum fermentation medium prepared by using resistant dextrin byproduct and system |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5580763A (en) * | 1993-11-08 | 1996-12-03 | Shin-Etsu Chemical Co., Ltd. | Method for fermentation production of xanthan gum |
| CN101560537A (en) * | 2009-05-19 | 2009-10-21 | 四川恒益科技有限公司 | Fermentation method for producing high viscocity xanthan gum by xanthomonas campestris |
| CN103509844A (en) * | 2013-08-09 | 2014-01-15 | 新疆阜丰生物科技有限公司 | Low-acyl gellan gum extracting method |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5580763A (en) * | 1993-11-08 | 1996-12-03 | Shin-Etsu Chemical Co., Ltd. | Method for fermentation production of xanthan gum |
| CN101560537A (en) * | 2009-05-19 | 2009-10-21 | 四川恒益科技有限公司 | Fermentation method for producing high viscocity xanthan gum by xanthomonas campestris |
| CN103509844A (en) * | 2013-08-09 | 2014-01-15 | 新疆阜丰生物科技有限公司 | Low-acyl gellan gum extracting method |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111187796A (en) * | 2019-01-28 | 2020-05-22 | 健隆生物科技股份有限公司 | Xanthan gum fermentation medium prepared by using resistant dextrin byproduct and system |
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