CN107271602A - A kind of device for eliminating thin-layer chromatography edge effect - Google Patents
A kind of device for eliminating thin-layer chromatography edge effect Download PDFInfo
- Publication number
- CN107271602A CN107271602A CN201710412477.4A CN201710412477A CN107271602A CN 107271602 A CN107271602 A CN 107271602A CN 201710412477 A CN201710412477 A CN 201710412477A CN 107271602 A CN107271602 A CN 107271602A
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- Prior art keywords
- side frame
- edge
- layer chromatography
- edge effect
- thin
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/90—Plate chromatography, e.g. thin layer or paper chromatography
- G01N30/92—Construction of the plate
- G01N30/93—Application of the sorbent layer
Abstract
The present invention relates to a kind of device for eliminating thin-layer chromatography edge effect, including two side frames, the rear baffles between side frame, side frame is towards scribbling one layer of easy hygroscopic material coating, fixing button of the side frame provided with fixed film plate on the face of rear baffle.The present apparatus is directly deployed without saturation, does not produce edge effect, and correspondence spot RfValue is consistent, linearly, is easy to observation to compare, and reappearance is good, while also saving the time.The present apparatus is applied to series of thin layers chromatography and differentiates the Chinese medicine true and false and the of inferior quality field of Functionality, quality and appealing design, good result is achieved.
Description
Technical field
The present invention relates to a kind of device for eliminating thin-layer chromatography edge effect, for laboratory TCD identificafion, quality analysis
And the field such as chemical analysis.
Background technology
Thin-layer chromatographic analysis technological invention so far, has been widely used for TCD identificafion, new drug development and chemical analysis etc.
Field, and gone through an edition Chinese Pharmacopoeia and record, for Chinese medicine, the prepared slices of Chinese crude drugs, Chinese medicine preparation, Chinese medical extract discriminating, be mirror
The important means of the other Chinese medicine true and false and quality good or not.Existing thin-layer chromatographic analysis equipment, due to itself design defect, generation it is thin
The problems such as layer chromatography edge effect and stability are poor, reappearance is bad, annoyings practitioner all the time, has had a strong impact on thin layer
The technology status of chromatography, while also limit the application of thin-layer chromatographic analysis technology.Asked to solve edge effect
Topic, practitioner also attempts to paste the methods such as filter paper bar to improve edge effect using such as presaturation, at the edge of developing tank, but
It is, due to the change of saturation time length, the factor such as the change of lamellae placement location so that operation lacks specification, causes
Solvent is vaporized degree and changed, and each solvent burden ratio of composition solvent changes, and solvent polarity occurs during expansion
Change etc., so that influence is produced on expansion result, while also bringing the results such as the stability difference of thin-layer chromatographic analysis.
The content of the invention
For the defect of prior art, the present invention provides a kind of device for eliminating thin-layer chromatography edge effect, present apparatus energy
Thin-layer chromatography edge effect is enough eliminated, increases the stability of thin-layer chromatographic analysis.
In order to solve the technical problem, the technical solution adopted by the present invention is：Including two side frames, positioned at side frame
Between rear baffle, side frame is thin provided with fixation towards one layer of easy hygroscopic material coating, side frame is scribbled on the face of rear baffle
The fixing button of laminate.
The device of the present invention for eliminating thin-layer chromatography edge effect, between the leading edge of two side frames and/or back edge
Between fixed strip is installed.
Fixed support is installed on the device of the present invention for eliminating thin-layer chromatography edge effect, side frame.
It is of the present invention eliminate thin-layer chromatography edge effect device, easy hygroscopic material coating be silica gel, alundum (Al2O3),
One kind in polyamide, polyimides, filter paper, the thickness of easy hygroscopic material coating is 0.2-2.0mm.
The device of the present invention for eliminating thin-layer chromatography edge effect, easy hygroscopic material coating extends to side from side frame lower edge
Rims upside edge leaves certain distance between top edge.
The device of the present invention for eliminating thin-layer chromatography edge effect, side frame is strip wide at the top and narrow at the bottom, side frame
Leading edge it is vertical with top edge, back edge and top edge are at an acute angle to be tilted, and the lower end forward edge bending of back edge makes
Side frame bottom is narrowed.
The device of the present invention for eliminating thin-layer chromatography edge effect, rear baffle is located at side frame bottom and narrows the upper of place
Side.
The device of the present invention for eliminating thin-layer chromatography edge effect, fixing button is the fixation on chip for protruding from side frame
Thing, it is fixed to turn round the space that holding and expanding layer is left between the back edge of side frame.
The device of the present invention for eliminating thin-layer chromatography edge effect, fixed torsion has 4, and 2 are located at two side frames respectively
Top, in addition 2 respectively be located at two side frames bottom.
Beneficial effects of the present invention：The present apparatus is directly deployed without saturation, does not produce edge effect, and correspondence spot RfValue
Unanimously, linearly, it is easy to observation to compare, reappearance is good, while also saving the time.The present apparatus is applied to series of thin layers
Chromatography differentiates the Chinese medicine true and false and the of inferior quality field of Functionality, quality and appealing design, achieves good result.
Brief description of the drawings
Fig. 1 is dimensional structure diagram of the invention；
Fig. 2 is left view structural representation of the invention；
Fig. 3 is the state diagram that the present apparatus is positioned in developing tank；
Fig. 4 is the chromatography figure of the unused present apparatus under visible ray；
Fig. 5 is the chromatography figure of the unused present apparatus under ultraviolet light；
Fig. 6 is the chromatography figure under visible ray using the present apparatus；
Fig. 7 is the chromatography figure under ultraviolet light using the present apparatus；
Chromatography figure when Fig. 8 is repeated experiment under visible ray；
Chromatography figure I when Fig. 9 is repeated experiment under ultraviolet light；
Chromatography figure II when Figure 10 is repeated experiment under ultraviolet light；
Chromatography figure III when Figure 11 is repeated experiment under ultraviolet light；
In figure：1st, side frame, 2, fixing button, 3, fixed strip, 4, fixed support, 5, rear baffle, 6, easy hygroscopic material coating, 7, exhibition
Fluting, 8, solvent.
Embodiment
The present invention is described further with specific embodiment below in conjunction with the accompanying drawings.
As shown in Figure 1, 2, a kind of device for eliminating thin-layer chromatography edge effect, including two side frames 1, positioned at side frame
Rear baffle 5 between 1, side frame 1 is towards one layer of easy hygroscopic material coating 6 is scribbled on the face of rear baffle 2, and side frame 1 is provided with
It is provided between the fixing button 2 of fixed film plate, the leading edge of two side frames 1 between back edge on fixed strip 3, side frame 1
Fixed support 4 is installed.
In the present embodiment, side frame 1 is strip wide at the top and narrow at the bottom, and the leading edge of side frame 1 is vertical with top edge, back
Edge and top edge inclination at an acute angle, and the lower end forward edge bending of back edge makes side frame bottom narrow to adapt to existing layer
Analyse the shape of groove.The easily hygroscopic materials such as the inner side of side frame applies upwards along lower edge, the silica gel of viscous suitable distance as coating,
Deploy upwards in expansion process floating coat immersion solvent, because the diffusion of solvent makes its periphery form gasification layer, its position
It is close to lamellae edge, it is suppressed that the excessive volatilization of sheet edges solvent so that sheet edges solvent during expansion
Evaporative effect and thin plate in the middle part of etc. other positions it is consistent, so as to eliminate the generation of the edge effect phenomenon of lamellae；
Fixing button 2 is set on side frame 1, for position of the fixed film plate in the present apparatus, so as to add thin-layer chromatography result
Uniformity, improves the stability of thin-layered chromatography；Rear baffle 5 is installed in the back edge of side frame 1, for controlling chromatography process
The spatial dimension of middle solvent evaporation, has certain effect, while also ensure that thin-layer chromatography result to reducing edge effect
Uniformity；Install fixed strip 3 additional between back edge between the leading edge of side frame, improve the stabilization of present apparatus structure, be allowed to
It is unlikely to deform, meanwhile, the fixed strip 3 between back edge also has fixation to lamellae；Side frame in side or both sides
Mounting and fixing bracket 4 in back edge, the present apparatus is fixed on before chromatography cylinder, it is ensured that the present apparatus reuse uniformity and
The stability of thin-layer chromatographic analysis result.
In the present embodiment, easy hygroscopic material coating is silica gel, and the thickness of silica gel is 1mm.Easy hygroscopic material is under side frame
Edge extends to side frame top edge or certain distance is left between top edge.
In the present embodiment, fixing button is the fixture on chip for protruding from side frame, it is fixed turn round with the back edge of side frame it
Between leave holding and expanding layer space.Fixed torsion has 4,2 be located at two side frames respectively top, 2 are located at respectively in addition
The bottom of two side frames.
In the present embodiment, rear baffle 5 is located at the top that side frame bottom narrows place；Support bracket fastened length and chromatography cylinder
Width is identical.
According to the size of developing tank, the device of the elimination thin-layer chromatography edge effect can make plurality of specifications.Including
Following specification, but following specification is not limited.Such as 10*10cm developing tank, the specific size of the present apparatus is：The height of side frame
Spend for 10.5cm, width is 2-2.3cm, and easy hygroscopic material coating 6 is upwardly extended from side frame lower edge, and its height is
10.5cm, the height of rear baffle is 8cm, and width is 11cm, and the length of fixed strip is 11cm, and fixed support length is 4cm.For
10*20cm developing tank, the specific size of the present apparatus is：The height of side frame is 18.5cm, and width is 2-2.5cm, easy moisture absorption
Material coating 6 is upwardly extended from side frame lower edge, and its height is 10.5cm, and the height of rear baffle is 10cm, and width is
11cm, the length of fixed strip is 11c, and fixed support length is 3cm.For 20*10cm developing tank, the specific size of the present apparatus
For：The height of side frame is 18.5cm, and width is 2-2.3cm, and easy hygroscopic material coating 6 is upwardly extended from side frame lower edge,
Its height is 10.5cm, and the height of rear baffle is 8cm, and width is 21cm, and the length of fixed strip is 21cm, and fixed support length is
4cm.For 20*20cm developing tank, the specific size of the present apparatus is：The height of side frame is 18.5cm, and width is 2-
2.3cm, easy hygroscopic material coating 6 is upwardly extended from side frame lower edge, and its height is 18.5cm, and the height of rear baffle is
10cm, width is 21cm, and the length of fixed strip is 21cm, and fixed support length is 4cm.
As shown in figure 3, during using the present apparatus, suitable developing tank 7 is selected first, during expansion, by the exhibition configured
Open agent 8 to pour into developing tank 7, the present apparatus is fixed on the front end of developing tank 7, both sides coating lower end is deep into solvent 8, will
The lamellae of expansion is needed, is placed into along peg button in the present apparatus, is deployed, remaining all operations are with Thin layer chromatography one
Sample.
As shown in Figure 4,5, it is the result directly deployed in visible ray and ultraviolet light without using the present apparatus, edge effect is very
Substantially.As shown in Figure 6,7, it is the result directly deployed in visible ray and ultraviolet light using the present apparatus, edge effect is substantially eliminated.
Using same batch sample, repeated experiment is carried out, when Fig. 8 is stability experiment, it is seen that under light, spot is linearly arranged,
Boundless edge effect；When Fig. 9 is stability experiment, under ultraviolet light, edge effect is not obvious；Figure 10,11 and Fig. 7 use same batch
Product, major spot is corresponded to always substantially.Therefore directly deployed using the present apparatus, as a result had good stability.
Described above is only the general principle and preferred embodiment of the present invention, and those skilled in the art do according to the present invention
The improvement and replacement gone out, belongs to the protection of the present invention.
Claims (9)
1. a kind of device for eliminating thin-layer chromatography edge effect, it is characterised in that：Including two side frames, between side frame
Rear baffle, side frame is provided with fixed film plate towards one layer of easy hygroscopic material coating, side frame is scribbled on the face of rear baffle
Fixing button.
2. the device according to claim 1 for eliminating thin-layer chromatography edge effect, it is characterised in that：The front of two side frames
Fixed strip is installed between edge and/or between back edge.
3. the device according to claim 1 for eliminating thin-layer chromatography edge effect, it is characterised in that：It is provided with side frame
Fixed support.
4. the device according to claim 1 for eliminating thin-layer chromatography edge effect, it is characterised in that：Easy hygroscopic material coating
For one kind in silica gel, alundum (Al2O3), polyamide, polyimides, filter paper, the thickness of easy hygroscopic material coating is 0.2-
2.0mm。
5. the device of the elimination thin-layer chromatography edge effect according to claim 1 or 4, it is characterised in that：Easy hygroscopic material
Coating extends to side frame top edge from side frame lower edge or certain distance is left between top edge.
6. the device according to claim 1 for eliminating thin-layer chromatography edge effect, it is characterised in that：Side frame is under upper width
Narrow strip, the leading edge of side frame is vertical with top edge, back edge and top edge inclination at an acute angle, and under back edge
End forward edge bending narrows side frame bottom.
7. the device according to claim 6 for eliminating thin-layer chromatography edge effect, it is characterised in that：Rear baffle is located at side
Frame bottom narrows the top at place.
8. the device according to claim 1 for eliminating thin-layer chromatography edge effect, it is characterised in that：Fixing button is to protrude from
The sheet fixture of side frame, leaves the space of holding and expanding layer between fixing button and the back edge of side frame.
9. the device according to claim 1 for eliminating thin-layer chromatography edge effect, it is characterised in that：Fixing button has 4,2
It is individual respectively be located at two side frames top, in addition 2 respectively be located at two side frames bottom.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710412477.4A CN107271602B (en) | 2017-06-05 | 2017-06-05 | A kind of device for eliminating thin-layer chromatography edge effect |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710412477.4A CN107271602B (en) | 2017-06-05 | 2017-06-05 | A kind of device for eliminating thin-layer chromatography edge effect |
Publications (2)
| Publication Number | Publication Date |
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| CN107271602A true CN107271602A (en) | 2017-10-20 |
| CN107271602B CN107271602B (en) | 2019-02-15 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201710412477.4A Active CN107271602B (en) | 2017-06-05 | 2017-06-05 | A kind of device for eliminating thin-layer chromatography edge effect |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112326863A (en) * | 2020-10-09 | 2021-02-05 | 北京科技大学 | Simple preparation plate for thin-layer chromatography separation and using method |
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| CN2200184Y (en) * | 1994-05-16 | 1995-06-07 | 甘肃省政法学院 | Thin bedded chromatographic analysis meter |
| US20040020834A1 (en) * | 2002-08-02 | 2004-02-05 | Bionisis | Apparatus for separating sample components by liquid chromatography under pressure |
| CN103424502A (en) * | 2012-05-17 | 2013-12-04 | 四川逢春制药有限公司 | Thin-layer chromatography expansion tank |
| CN104111304A (en) * | 2014-07-15 | 2014-10-22 | 大连大学 | Efficient intelligent automatic unfolding device |
| CN205879895U (en) * | 2016-06-14 | 2017-01-11 | 国家烟草质量监督检验中心 | Thin -layer chromatography expandes device and thin layer plate adjusting mount thereof |
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2017
- 2017-06-05 CN CN201710412477.4A patent/CN107271602B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2200184Y (en) * | 1994-05-16 | 1995-06-07 | 甘肃省政法学院 | Thin bedded chromatographic analysis meter |
| US20040020834A1 (en) * | 2002-08-02 | 2004-02-05 | Bionisis | Apparatus for separating sample components by liquid chromatography under pressure |
| CN103424502A (en) * | 2012-05-17 | 2013-12-04 | 四川逢春制药有限公司 | Thin-layer chromatography expansion tank |
| CN104111304A (en) * | 2014-07-15 | 2014-10-22 | 大连大学 | Efficient intelligent automatic unfolding device |
| CN205879895U (en) * | 2016-06-14 | 2017-01-11 | 国家烟草质量监督检验中心 | Thin -layer chromatography expandes device and thin layer plate adjusting mount thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112326863A (en) * | 2020-10-09 | 2021-02-05 | 北京科技大学 | Simple preparation plate for thin-layer chromatography separation and using method |
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| CN107271602B (en) | 2019-02-15 |
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