CN107271505A - Composite electrode and preparation method thereof, Quercetin sensor - Google Patents
Composite electrode and preparation method thereof, Quercetin sensor Download PDFInfo
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- CN107271505A CN107271505A CN201710404072.6A CN201710404072A CN107271505A CN 107271505 A CN107271505 A CN 107271505A CN 201710404072 A CN201710404072 A CN 201710404072A CN 107271505 A CN107271505 A CN 107271505A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
- G01N27/308—Electrodes, e.g. test electrodes; Half-cells at least partially made of carbon
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Abstract
This application discloses a kind of composite electrode and preparation method thereof, Quercetin sensor, the composite electrode, including electrode matrix and the porous graphene layer and beta cyclodextrin film layer for being sequentially formed in electrode matrix surface.In the present invention, electro-deposition graphene has good electric conductivity and larger specific surface area, and it can provide substantial amounts of avtive spot and improve the transfer of electric charge.By further polymeric cyclodextrins film, it shows very strong oxidisability to Quercetin.Prepared sensor is 0.003 μM~25 μM to the linear response range of Quercetin.
Description
Technical field
The application is related to a kind of sensor, and more particularly to a kind of composite electrode and preparation method thereof, Quercetin are passed
Sensor.
Background technology
Quercetin is a kind of day with multiple biological activities such as anti-oxidant, antiviral, antitumor and regulation immunologic functions
Right flavone compound, is one of active ingredient in a variety of parts of generic medicinal plants such as honeysuckle, pseudo-ginseng, ginkgo and natural products,
With very high medical value.
Analyzed from its structure, Quercetin contains hydroxyl, possess the condition with function monomer formation hydrogen bond, but in molecule
Containing 5 hydroxyls, make its polarity larger and do not dissolve in nonpolar or weak polar solvent.
At present AAS, fluorimetry, high performance liquid chromatography are generally included for the assay method of Quercetin
Deng these methods generally have cost height, complex operation, the technical problem that time-consuming, sensitivity is low.
The content of the invention
It is an object of the invention to provide a kind of composite electrode and preparation method thereof, Quercetin sensor, to overcome
Deficiency of the prior art.
To achieve the above object, the present invention provides following technical scheme：
The embodiment of the present application discloses a kind of composite electrode, including electrode matrix and is sequentially formed in electrode matrix
The porous graphene layer and beta-schardinger dextrin film layer on surface.
It is preferred that, in above-mentioned composite electrode, the electrode matrix is glass-carbon electrode.
Accordingly, disclosed herein as well is a kind of preparation method of composite electrode, including step：
(1), graphene and graphene oxide are dispersed in phosphate buffer solution, graphene and graphene oxide is obtained
Mixed liquor；Using glass-carbon electrode as working electrode, the mixed liquor of the graphene and graphene oxide is entered using three-electrode system
Row cyclic voltammetry scan, obtains the glass-carbon electrode of porous graphene modification；
(2) the glass-carbon electrode immersion for, modifying porous graphene is dissolved with the phosphate buffer of beta-schardinger dextrin, in electricity
6 sections of electrochemical polymerization, obtain composite electrode between pressure -2V~2.5V.
It is preferred that, in the preparation method of above-mentioned composite electrode, in the step (2), the preparation of beta-schardinger dextrin
Method includes：Viscose rayon cellulose fiber is dispersed in NaOH solution, 2~3h is swelled；Added in viscose rayon to after being swelled
Epoxychloropropane and NaOH solution, are heated to 50~70 DEG C and are incubated 3~5 hours；Product is washed to neutrality, suction filtration；Acetone extracts
Take, beta-schardinger dextrin is made in dialysis treatment.
Disclosed herein as well is a kind of Quercetin sensor, working electrode is used as using described composite electrode.
Compared with prior art, the advantage of the invention is that：In the present invention, electro-deposition graphene has good electric conductivity
With larger specific surface area, it can provide substantial amounts of avtive spot and improve the transfer of electric charge.Pass through further polymeric rings
Dextrin film, it shows very strong oxidisability to Quercetin.Prepared sensor is to the linear response range of Quercetin
0.003 μM~25 μM.
Embodiment
The present invention is described further by the following example：According to following embodiments, the present invention may be better understood.
However, as it will be easily appreciated by one skilled in the art that specific material ratio, process conditions and its result described by embodiment are only used
In the explanation present invention, without should be also without limitation on the present invention described in detail in claims.
Graphene and graphene oxide are dispersed in phosphate buffer solution, the mixing of graphene and graphene oxide is obtained
Liquid；Using glass-carbon electrode as working electrode, the mixed liquor of the graphene and graphene oxide is followed using three-electrode system
Ring voltammetric scan, obtains the glass-carbon electrode of porous graphene modification.
Viscose rayon cellulose fiber is dispersed in NaOH solution, 2~3h is swelled；Ring is added in viscose rayon to after being swelled
Oxygen chloropropane and NaOH solution, are heated to 50~70 DEG C and are incubated 3~5 hours；Product is washed to neutrality, suction filtration；Acetone extract,
Beta-schardinger dextrin is made in dialysis treatment.
The glass-carbon electrode immersion that porous graphene is modified is dissolved with the phosphate buffer of beta-schardinger dextrin, in voltage -2V
6 sections of electrochemical polymerization, obtain composite electrode between~2.5V.
Quercetin sensor is made using composite electrode as working electrode.
In the present embodiment, electro-deposition graphene has good electric conductivity and larger specific surface area, and it can be provided greatly
The avtive spot of amount and the transfer for improving electric charge.By further polymeric cyclodextrins film, it shows very strong to Quercetin
Oxidisability.Prepared sensor is 0.003 μM~25 μM to the linear response range of Quercetin.
Finally, in addition it is also necessary to explanation, term " comprising ", "comprising" or its any other variant are intended to non-exclusive
Property include so that process, method, article or equipment including a series of key elements not only include those key elements, and
Also include other key elements for being not expressly set out, or also include for this process, method, article or equipment inherently
Key element.
Claims (5)
1. a kind of composite electrode, it is characterised in that including electrode matrix and be sequentially formed in many of electrode matrix surface
Hole graphene layer and beta-schardinger dextrin film layer.
2. composite electrode according to claim 1, it is characterised in that：The electrode matrix is glass-carbon electrode.
3. the preparation method of the composite electrode described in claim 1 or 2, it is characterised in that including step：
(1), graphene and graphene oxide are dispersed in phosphate buffer solution, the mixing of graphene and graphene oxide is obtained
Liquid；Using glass-carbon electrode as working electrode, the mixed liquor of the graphene and graphene oxide is followed using three-electrode system
Ring voltammetric scan, obtains the glass-carbon electrode of porous graphene modification；
(2) the glass-carbon electrode immersion for, modifying porous graphene is dissolved with the phosphate buffer of beta-schardinger dextrin, in voltage -2V
6 sections of electrochemical polymerization, obtain composite electrode between~2.5V.
4. the preparation method of composite electrode according to claim 3, it is characterised in that：In the step (2), β-ring
The preparation method of dextrin includes：Viscose rayon cellulose fiber is dispersed in NaOH solution, 2~3h is swelled；Viscose glue to after being swelled
Epoxychloropropane and NaOH solution are added in fiber, 50~70 DEG C is heated to and is incubated 3~5 hours；Product is washed to neutrality, is taken out
Filter；Beta-schardinger dextrin is made in acetone extract, dialysis treatment.
5. a kind of Quercetin sensor, it is characterised in that work electricity is used as using the composite electrode described in claim 1 or 2
Pole.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710404072.6A CN107271505A (en) | 2017-06-01 | 2017-06-01 | Composite electrode and preparation method thereof, Quercetin sensor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201710404072.6A CN107271505A (en) | 2017-06-01 | 2017-06-01 | Composite electrode and preparation method thereof, Quercetin sensor |
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| CN107271505A true CN107271505A (en) | 2017-10-20 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201710404072.6A Pending CN107271505A (en) | 2017-06-01 | 2017-06-01 | Composite electrode and preparation method thereof, Quercetin sensor |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108744591A (en) * | 2018-04-20 | 2018-11-06 | 中国石油化工股份有限公司 | The extracting process of chromocor compound |
| CN110586640A (en) * | 2019-10-26 | 2019-12-20 | 闫娜 | Method for removing and recovering heavy metals in polluted soil |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104652127A (en) * | 2013-11-20 | 2015-05-27 | 富强 | Synthetic method of [beta]-cyclodextrin |
| CN104947134A (en) * | 2015-07-13 | 2015-09-30 | 湖南农业大学 | Preparation method of porous graphene |
| CN106525932A (en) * | 2016-11-02 | 2017-03-22 | 红河学院 | Thiamphenicol molecularly imprinted electrochemical sensor and preparation method and application thereof |
-
2017
- 2017-06-01 CN CN201710404072.6A patent/CN107271505A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104652127A (en) * | 2013-11-20 | 2015-05-27 | 富强 | Synthetic method of [beta]-cyclodextrin |
| CN104947134A (en) * | 2015-07-13 | 2015-09-30 | 湖南农业大学 | Preparation method of porous graphene |
| CN106525932A (en) * | 2016-11-02 | 2017-03-22 | 红河学院 | Thiamphenicol molecularly imprinted electrochemical sensor and preparation method and application thereof |
Non-Patent Citations (2)
| Title |
|---|
| LU, XIA ET AL.: "Molecularly imprinted electrochemical sensor based on an electrode modified with an imprinted pyrrole film immobilized on a β-cyclodextrin/gold nanoparticles/graphene layer", 《RSC ADVANCES》 * |
| ZHANG, GU ET AL.: "Mild and novel electrochemical preparation of β-cyclodextrin/graphene nanocomposite film for super-sensitive sensing of quercetin", 《BIOSENSORS & BIOELECTRONICS》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108744591A (en) * | 2018-04-20 | 2018-11-06 | 中国石油化工股份有限公司 | The extracting process of chromocor compound |
| CN110586640A (en) * | 2019-10-26 | 2019-12-20 | 闫娜 | Method for removing and recovering heavy metals in polluted soil |
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