CN107259064A - A kind of production method for hydrolyzing fish soluble slurry protein powder - Google Patents
A kind of production method for hydrolyzing fish soluble slurry protein powder Download PDFInfo
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- CN107259064A CN107259064A CN201710562320.XA CN201710562320A CN107259064A CN 107259064 A CN107259064 A CN 107259064A CN 201710562320 A CN201710562320 A CN 201710562320A CN 107259064 A CN107259064 A CN 107259064A
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- 241000251468 Actinopterygii Species 0.000 title claims abstract description 64
- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 60
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 60
- 239000002002 slurry Substances 0.000 title claims abstract description 60
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 33
- 239000000843 powder Substances 0.000 title claims abstract description 33
- 230000003301 hydrolyzing effect Effects 0.000 title claims abstract description 19
- 108091005804 Peptidases Proteins 0.000 claims abstract description 76
- 239000004365 Protease Substances 0.000 claims abstract description 51
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims abstract description 51
- 235000019419 proteases Nutrition 0.000 claims abstract description 51
- 210000000496 pancreas Anatomy 0.000 claims abstract description 49
- 108090000317 Chymotrypsin Proteins 0.000 claims abstract description 44
- 229960002376 chymotrypsin Drugs 0.000 claims abstract description 44
- 239000000796 flavoring agent Substances 0.000 claims abstract description 32
- 235000019634 flavors Nutrition 0.000 claims abstract description 32
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- 230000000694 effects Effects 0.000 claims abstract description 28
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- 235000019833 protease Nutrition 0.000 claims abstract description 25
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- 230000007062 hydrolysis Effects 0.000 claims abstract description 17
- 238000006460 hydrolysis reaction Methods 0.000 claims abstract description 17
- 238000000034 method Methods 0.000 claims abstract description 9
- 230000008569 process Effects 0.000 claims abstract description 6
- 238000001694 spray drying Methods 0.000 claims abstract description 6
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- 125000003275 alpha amino acid group Chemical group 0.000 claims 1
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- QCVGEOXPDFCNHA-UHFFFAOYSA-N 5,5-dimethyl-2,4-dioxo-1,3-oxazolidine-3-carboxamide Chemical compound CC1(C)OC(=O)N(C(N)=O)C1=O QCVGEOXPDFCNHA-UHFFFAOYSA-N 0.000 description 5
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- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 description 1
- WCTCIIAGNMFYAO-DCAQKATOSA-N Leu-Cys-Val Chemical compound [H]N[C@@H](CC(C)C)C(=O)N[C@@H](CS)C(=O)N[C@@H](C(C)C)C(O)=O WCTCIIAGNMFYAO-DCAQKATOSA-N 0.000 description 1
- WRLPVDVHNWSSCL-MELADBBJSA-N Leu-His-Pro Chemical compound CC(C)C[C@@H](C(=O)N[C@@H](CC1=CN=CN1)C(=O)N2CCC[C@@H]2C(=O)O)N WRLPVDVHNWSSCL-MELADBBJSA-N 0.000 description 1
- 108010070551 Meat Proteins Proteins 0.000 description 1
- GXCLVBGFBYZDAG-UHFFFAOYSA-N N-[2-(1H-indol-3-yl)ethyl]-N-methylprop-2-en-1-amine Chemical compound CN(CCC1=CNC2=C1C=CC=C2)CC=C GXCLVBGFBYZDAG-UHFFFAOYSA-N 0.000 description 1
- ZUXQFMVPAYGPFJ-JXUBOQSCSA-N Thr-Ala-Lys Chemical compound C[C@@H](O)[C@H](N)C(=O)N[C@@H](C)C(=O)N[C@H](C(O)=O)CCCCN ZUXQFMVPAYGPFJ-JXUBOQSCSA-N 0.000 description 1
- QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 description 1
- XJFXZQKJQGYFMM-GUBZILKMSA-N Val-Cys-Val Chemical compound CC(C)[C@@H](C(=O)N[C@@H](CS)C(=O)N[C@@H](C(C)C)C(=O)O)N XJFXZQKJQGYFMM-GUBZILKMSA-N 0.000 description 1
- 238000005903 acid hydrolysis reaction Methods 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 239000011149 active material Substances 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000013475 authorization Methods 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000000711 cancerogenic effect Effects 0.000 description 1
- 231100000357 carcinogen Toxicity 0.000 description 1
- 239000003183 carcinogenic agent Substances 0.000 description 1
- -1 chloro- 2- propyl Chemical group 0.000 description 1
- 108010016616 cysteinylglycine Proteins 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000006866 deterioration Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000002845 discoloration Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 235000013601 eggs Nutrition 0.000 description 1
- 238000004945 emulsification Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 235000013350 formula milk Nutrition 0.000 description 1
- 235000013376 functional food Nutrition 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 108010040030 histidinoalanine Proteins 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 235000008446 instant noodles Nutrition 0.000 description 1
- 235000013622 meat product Nutrition 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- BDERNNFJNOPAEC-UHFFFAOYSA-N n-propyl alcohol Natural products CCCO BDERNNFJNOPAEC-UHFFFAOYSA-N 0.000 description 1
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- 238000005457 optimization Methods 0.000 description 1
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- 230000007065 protein hydrolysis Effects 0.000 description 1
- 230000020978 protein processing Effects 0.000 description 1
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- 230000033764 rhythmic process Effects 0.000 description 1
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- 210000004885 white matter Anatomy 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/04—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from fish or other sea animals
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/20—Removal of unwanted matter, e.g. deodorisation or detoxification
- A23L5/27—Removal of unwanted matter, e.g. deodorisation or detoxification by chemical treatment, by adsorption or by absorption
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/20—Removal of unwanted matter, e.g. deodorisation or detoxification
- A23L5/27—Removal of unwanted matter, e.g. deodorisation or detoxification by chemical treatment, by adsorption or by absorption
- A23L5/273—Removal of unwanted matter, e.g. deodorisation or detoxification by chemical treatment, by adsorption or by absorption using adsorption or absorption agents, resins, synthetic polymers, or ion exchangers
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Seasonings (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention discloses a kind of production method for hydrolyzing fish soluble slurry protein powder, hydrolysis includes pancreas chymotrypsin and flavor protease stepwise discretization, infrared ray temperature control is used in enzymolysis process, and add proteinase activated dose while pancreas chymotrypsin enzymolysis is added, enzymolysis terminates rear dialysis, spray drying after filtered solution, activated carbon and alcohol deodorization is taken to obtain albumen powder.Have the beneficial effect that：The present invention uses pancreas chymotrypsin and flavor protease stepwise discretization, and both arrange in pairs or groups science, and enzymolysis efficiency is high；The time of enzymolysis can be greatly reduced using infrared ray temperature control, shorten life cycle of the product, reduction cost of labor and time cost；Associated proteins zymoexciter is digested, and proteinase activated dose can improve the activity of protease, is reduced albumen enzyme dosage and is shortened enzymolysis time, reduces production cost；Production method of the present invention, simple to operate, required equipment is few, with short production cycle, and reappearance is high, good product quality, is adapted to large-scale production.
Description
Technical field
The present invention relates to food processing technology field, specifically a kind of production method for hydrolyzing fish soluble slurry protein powder.
Background technology
Protein hydrolyzate mainly contains various polypeptides and amino acid, and tool has been widely used, can apply to functional food,
There is provided the nutritional ingredient of food, improvement food in the processing such as formula milk, flavoring, meat products, instant noodles and bakery
Delicate flavour mouthfeel, the precursor for providing Mei Lade thermal responses, strengthen the physical characteristics such as the emulsification and retentiveness of food, play antioxygen
Change effect etc..
Early protein is hydrolyzed using hydrochloric acid, and degree of hydrolysis is very high, but such as tryptophan destruction of some amino acid is serious, there is serious
Acidifying taste, while produce carcinogen 1, the chloro- 2- propyl alcohol of 3- bis- and chloro- 1, the 2- propane diols of 3- etc..With albumen
Zymolysis technique is progressively ripe, replaces sour hydrolysate to turn into trend using animal/vegetable protein raw material enzymolysis production protolysate.
Animal protein is that polypeptide, the acid hydrolysis thing of meat proteins enzymolysis production have been widely used.But protease cost compared with
Height, which increase cost prepared by albumen powder, and the difference of protein molecular weight, function is very different.
With the deterioration of environment, the quickening of rhythm of life and the raising of health care consciousness, people to the concern of health gradually
Sub-health state is extended to from disease, therefore the extraction of the active component with healthcare function prepares the weight for being increasingly subject to consumer
Depending on.On the other hand, along with the development and the improvement of people's living standards of science and technology, people are to active ingredient in marine resources
Understanding deepen continuously, to marine organisms originate active material demand be continuously increased.
Protein shortage is still the Tough questions that the whole world faces, according to the number of Food and Agricultural Organization of the United Nations
According to annual about 1800-2,500 ten thousand tons of whole world edible protein shortage.With being continuously increased for the size of population, to animal egg
The demand of white matter is also increasing, but the resource-constrained of terrestrial animal albumen, the need for can not meet population rapid growth,
Commercial Fishes Resources are also failed constantly simultaneously, and low value fish crop is being continuously increased, therefore are increased to Optimization of Low Value Fish
Research and utilization, the functional protein product for developing high added value is necessary.Fish protein is generally acknowledged best high-quality animal
Protein, its hydrolysate free aminoacid content is high, and ratio is reasonable, it is necessary to which amino acid content can exceed that FDA/WHO is marked
Quasi-mode.
Prior art such as Authorization Notice No. is the B of CN 102077897 Chinese invention patent, discloses a kind of fish and water solution egg
White powder preparation method and its a kind of fish protein hydrolysate powder of preparation, belong to food protein and food protein processing technique field
；Fish and water solution protein powder preparation method step is：First determine for hydrolyzing Fish raw materials, then with the fish raw material protease of itself
It is hydrolyzed, finally hydrolyzate heating is made to isolate hydrolyzate after enzyme-deactivating again, be concentrated and dried, finally obtained fish and water solution egg
White powder.Albumen powder enzymolysis prepared by this method is insufficient, and enzymolysis time is longer, and the production cycle is longer.
The content of the invention
It is an object of the invention to provide a kind of molten slurry protein hydrolysis of fish is abundant, protein molecule is small, rapid enzymolysis and
It is few with enzyme amount, the production method of the colourless hydrolysis fish soluble slurry protein powder without fishy smell.
The present invention is directed to the problem of being mentioned in background technology, and the technical scheme taken is：One kind hydrolysis fish soluble slurry protein powder
Production method, hydrolysis include pancreas chymotrypsin and flavor protease stepwise discretization, addition pancreas chymotrypsin while add
Zymoexciter.The molten slurry of fish adds water dilution, the molten slurry of fish before protease hydrolyzed is added：Water=1:20~40v/v.Pancreas chymotrypsin and
Activity is higher in the molten slurry of the fish of flavor protease after dilution.
Preferably, pancreas chymotrypsin addition is 0.6 ~ 2.0% of the molten volume of slurry of fish before dilution, proteinase activated dose
Addition is 0.001 ~ 0.004% of the molten volume of slurry of fish before dilution.Pancreas chymotrypsin hydrolysis temperature be 48 ~ 60 DEG C, pH be 7.5 ~
9.0, enzymolysis time is 8 ~ 15min.Pancreas chymotrypsin enzymolysis is gone out 4 ~ 8min of enzyme after terminating using boiling water bath, addition local flavor albumen
Enzyme, addition is 0.3 ~ 1.2% of the molten volume of slurry of fish before dilution.Flavor protease hydrolysis temperature be 48 ~ 60 DEG C, pH be 5.6 ~
6.7, enzymolysis time is 8 ~ 15min.Pancreas chymotrypsin and flavor protease stepwise discretization, the molecular weight that enzymolysis is obtained are small, enzymolysis
Fully.Pancreas chymotrypsin is tentatively digested to protein in the molten slurry of fish, more restriction enzyme sites is exposed, local flavor albumen
Enzyme continues to digest, and will tentatively digest obtained protein and further digests as smaller molecule, and both arrange in pairs or groups science, enzymolysis efficiency
It is high.
Preferably, using infrared ray temperature control in enzymolysis process, the enzyme that gone out after terminating is digested, using 2000 ~ 5000Da's
Half membrane dialyzing, takes spray drying after filtered solution, activated carbon and alcohol deodorization to obtain albumen powder.Can be significantly using infrared ray temperature control
The time of enzymolysis is reduced, shortens life cycle of the product, reduction cost of labor and time cost.Half membrane dialyzing can be removed effectively greatly
Molecule protein and macromolecular other impurities, slurry albumen molten to fish are purified.The addition of activated carbon for filtered solution 0.5 ~
1.5%w/v, temperature is 34 ~ 42 DEG C, and the decoloration deodorization time is 20 ~ 50min.Activated carbon has fine and close pore structure, to fishy smell
Material and pigment have excellent adsorption effect.Alcohol addition be filtered solution in the final concentration of 4 ~ 8%v/v of alcohol, temperature be 17 ~
25 DEG C, the deodorant time is 14 ~ 25min.Alcohol has dissolution to fishy smell material, can effectively deodorization, and have covering to make fishy smell
With.
Preferably, proteinase activated dose of composition and its parts by weight are：8 ~ 10 parts of starch, 5 ~ 10 parts of konjaku flour, malt paste
3 ~ 6 parts of 15 ~ 20 parts of essence, 10 ~ 16 parts of maltitol and active protein peptide, the amino acid sequence of wherein active protein peptide is
HSHACKLCVCVNCFCGTAKCTHCRVLHPGKLCVCVNCSK.Above-mentioned proteinase activated dose produces tension force effect to substrate, makes
Substrate is distorted, and promotes ES to enter activated state, and can improve the activity of protease, is improved the enzymolysis efficiency of protease, is reduced egg
White enzyme dosage simultaneously shortens enzymolysis time, reduces production cost.There is hydrogen bond action between activated protein and protease, can change
The molecular conformation of protease, and cooperated with other compositions in proteinase activated dose, proteinase activity can be effectively improved.
Compared with prior art, the advantage of the invention is that：1）The present invention is using pancreas chymotrypsin and flavor protease point
Step enzymolysis, pancreas chymotrypsin is tentatively digested to protein in the molten slurry of fish, more restriction enzyme sites is exposed, local flavor egg
White enzyme continues to digest, and tentatively digest obtained protein, further enzymolysis is smaller molecule, and both arrange in pairs or groups science, digest and imitate
Rate is high；2）The time of enzymolysis can be greatly reduced using infrared ray temperature control, shorten life cycle of the product, reduction cost of labor and time
Cost；3）Associated proteins zymoexciter is digested, and proteinase activated dose can improve the activity of protease, is reduced albumen enzyme dosage and is contracted
Short enzymolysis time, reduces production cost；4）Discoloration deodorization, activity are carried out to fish soluble slurry protein powder using activated carbon and alcohol
Charcoal has fine and close pore structure, has excellent adsorption effect alcohol to have dissolving to make to fishy smell material to fishy smell material and pigment
With can effectively deodorization, and have covering effect to fishy smell；5）Production method of the present invention, simple to operate, required equipment is few, production week
Phase is short, and reappearance is high, good product quality, is adapted to large-scale production.
Specific embodiment
The present invention program is described further below by embodiment：
Embodiment 1：
A kind of production method for hydrolyzing fish soluble slurry protein powder, hydrolysis includes pancreas chymotrypsin and flavor protease stepwise discretization,
Proteinase activated dose is added while adding pancreas chymotrypsin.The molten slurry of fish adds water dilution before protease hydrolyzed is added, and fish is molten
Slurry：Water=1:20~40v/v.Activity is higher in the molten slurry of the fish of pancreas chymotrypsin and flavor protease after dilution.
Pancreas chymotrypsin addition is 0.6 ~ 2.0% of the molten volume of slurry of fish before dilution, and proteinase activated dose of addition is dilute
The 0.001 ~ 0.004% of the molten volume of slurry of fish before releasing.Pancreas chymotrypsin hydrolysis temperature is 48 ~ 60 DEG C, and pH is 7.5 ~ 9.0, enzymolysis time
For 8 ~ 15min.Pancreas chymotrypsin enzymolysis is gone out 4 ~ 8min of enzyme after terminating using boiling water bath, adds flavor protease, and addition is dilute
The 0.3 ~ 1.2% of the molten volume of slurry of fish before releasing.Flavor protease hydrolysis temperature be 48 ~ 60 DEG C, pH be 5.6 ~ 6.7, enzymolysis time be 8 ~
15min.Pancreas chymotrypsin and flavor protease stepwise discretization, the molecular weight that enzymolysis is obtained are small, and enzymolysis is abundant.Pancreas chymotrypsin pair
Protein is tentatively digested in the molten slurry of fish, and more restriction enzyme sites are exposed, and flavor protease continues to digest, will be preliminary
Digesting obtained protein, further enzymolysis is smaller molecule, and both arrange in pairs or groups science, and enzymolysis efficiency is high.
Infrared ray temperature control is used in enzymolysis process, the enzyme that gone out after terminating is digested, is oozed using 2000 ~ 5000Da pellicle
Analysis, takes spray drying after filtered solution, activated carbon and alcohol deodorization to obtain albumen powder.Enzymolysis can be greatly reduced using infrared ray temperature control
Time, shorten life cycle of the product, reduction cost of labor and time cost.Half membrane dialyzing can effectively remove high molecular weight protein
Matter and macromolecular other impurities, slurry albumen molten to fish are purified.The addition of activated carbon is 0.5 ~ 1.5%w/v of filtered solution,
Temperature is 34 ~ 42 DEG C, and the decoloration deodorization time is 20 ~ 50min.Activated carbon has fine and close pore structure, to fishy smell material and color
Have excellent adsorption effect.Alcohol addition is the final concentration of 4 ~ 8%v/v of alcohol in filtered solution, and temperature is 17 ~ 25 DEG C, deodorant
Time is 14 ~ 25min.Alcohol has dissolution to fishy smell material, can effectively deodorization, and have covering effect to fishy smell.
Proteinase activated dose of composition and its parts by weight are：8 ~ 10 parts of starch, 5 ~ 10 parts of konjaku flour, 15 ~ 20 parts of maltodextrin,
3 ~ 6 parts of 10 ~ 16 parts of maltitol and active protein peptide, the amino acid sequence of wherein active protein peptide is
HSHACKLCVCVNCFCGTAKCTHCRVLHPGKLCVCVNCSK.Above-mentioned proteinase activated dose produces tension force effect to substrate, makes
Substrate is distorted, and promotes ES to enter activated state, and can improve the activity of protease, is improved the enzymolysis efficiency of protease, is reduced egg
White enzyme dosage simultaneously shortens enzymolysis time, reduces production cost.There is hydrogen bond action between activated protein and protease, can change
The molecular conformation of protease, and cooperated with other compositions in proteinase activated dose, proteinase activity can be effectively improved.
Embodiment 2：
A kind of production method for hydrolyzing fish soluble slurry protein powder, hydrolysis includes pancreas chymotrypsin and flavor protease stepwise discretization,
Proteinase activated dose is added while adding pancreas chymotrypsin.The molten slurry of fish adds water dilution before protease hydrolyzed is added, and fish is molten
Slurry：Water=1:35v/v.Activity is higher in the molten slurry of the fish of pancreas chymotrypsin and flavor protease after dilution.
Pancreas chymotrypsin addition is 1.3% of the molten volume of slurry of fish before dilution, before proteinase activated dose of addition is dilution
The 0.003% of the molten volume of slurry of fish.Pancreas chymotrypsin hydrolysis temperature is 50 DEG C, and pH is 8.4, and enzymolysis time is 10min.Pancreas gruel albumen
Enzyme enzymolysis is gone out enzyme 5min after terminating using boiling water bath, adds flavor protease, and addition is the 0.9% of the molten volume of slurry of the preceding fish of dilution.
Flavor protease hydrolysis temperature is 55 DEG C, and pH is 6.0, and enzymolysis time is 10min.Pancreas chymotrypsin and flavor protease substep enzyme
Solution, the molecular weight that enzymolysis is obtained is small, and enzymolysis is abundant.Pancreas chymotrypsin is tentatively digested to protein in the molten slurry of fish, will be more
Restriction enzyme site be exposed, flavor protease continues to digest, and it is smaller for tentatively digest obtained protein and further digest
Molecule, both arrange in pairs or groups science, and enzymolysis efficiency is high.
Infrared ray temperature control is used in enzymolysis process, the enzyme that gone out after terminating is digested, using 4000Da half membrane dialyzing, takes filter
Cross spray drying after liquid, activated carbon and alcohol deodorization and obtain albumen powder.The time of enzymolysis can be greatly reduced using infrared ray temperature control,
Shorten life cycle of the product, reduction cost of labor and time cost.Half membrane dialyzing can effectively remove macro-molecular protein and big
Molecule other impurities, slurry albumen molten to fish is purified.The addition of activated carbon is the 1.0%w/v of filtered solution, and temperature is 38 DEG C,
The decoloration deodorization time is 40min.Activated carbon has fine and close pore structure, there is excellent absorption effect to fishy smell material and pigment
Really.Alcohol addition is the final concentration of 5%v/v of alcohol in filtered solution, and temperature is 22 DEG C, and the deodorant time is 20min.Alcohol is to raw meat
Taste material has dissolution, can effectively deodorization, and have covering effect to fishy smell.
Proteinase activated dose of composition and its parts by weight are：7 parts of starch, 8 parts of konjaku flour, 16 parts of maltodextrin, maltitol
14 parts are with 5 parts of active protein peptide, the amino acid sequence of wherein active protein peptide
HSHACKLCVCVNCFCGTAKCTHCRVLHPGKLCVCVNCSK.Above-mentioned proteinase activated dose produces tension force effect to substrate, makes
Substrate is distorted, and promotes ES to enter activated state, and can improve the activity of pancreas chymotrypsin, is improved the enzymolysis efficiency of protease, is subtracted
Few pancreas chymotrypsin consumption simultaneously shortens enzymolysis time, reduces production cost.There is hydrogen between activated protein and pancreas chymotrypsin
Key is acted on, and can change the molecular conformation of pancreas chymotrypsin, and is cooperated with other compositions in proteinase activated dose, can effectively be carried
High protein enzymatic activity.
Embodiment 3：
A kind of production method for hydrolyzing fish soluble slurry protein powder, hydrolysis includes pancreas chymotrypsin and flavor protease stepwise discretization.Fish
Molten slurry adds water dilution, the molten slurry of fish before protease hydrolyzed is added：Water=1:30v/v.Pancreas chymotrypsin and flavor protease are dilute
Activity is higher in the molten slurry of fish after releasing.
Pancreas chymotrypsin addition is 1.4% of the molten volume of slurry of fish before dilution, before proteinase activated dose of addition is dilution
The 0.003% of the molten volume of slurry of fish.Pancreas chymotrypsin hydrolysis temperature is 50 DEG C, and pH is 8.4, and enzymolysis time is 10min.Pancreas gruel albumen
Enzyme enzymolysis is gone out enzyme 5min after terminating using boiling water bath, adds flavor protease, and addition is the 0.7% of the molten volume of slurry of the preceding fish of dilution.
Flavor protease hydrolysis temperature is 55 DEG C, and pH is 6.0, and enzymolysis time is 10min.Pancreas chymotrypsin and flavor protease substep enzyme
Solution, the molecular weight that enzymolysis is obtained is small, and enzymolysis is abundant.Pancreas chymotrypsin is tentatively digested to protein in the molten slurry of fish, will be more
Restriction enzyme site be exposed, flavor protease continues to digest, and it is smaller for tentatively digest obtained protein and further digest
Molecule, both arrange in pairs or groups science, and enzymolysis efficiency is high.
Infrared ray temperature control is used in enzymolysis process, the enzyme that gone out after terminating is digested, using 4000Da half membrane dialyzing, takes filter
Cross spray drying after liquid, activated carbon and alcohol deodorization and obtain albumen powder.The time of enzymolysis can be greatly reduced using infrared ray temperature control,
Shorten life cycle of the product, reduction cost of labor and time cost.Half membrane dialyzing can effectively remove macro-molecular protein and big
Molecule other impurities, slurry albumen molten to fish is purified.The addition of activated carbon is the 1.0%w/v of filtered solution, and temperature is 38 DEG C,
The decoloration deodorization time is 40min.Activated carbon has fine and close pore structure, there is excellent absorption effect to fishy smell material and pigment
Really.Alcohol addition is the final concentration of 5%v/v of alcohol in filtered solution, and temperature is 22 DEG C, and the deodorant time is 20min.Alcohol is to raw meat
Taste material has dissolution, can effectively deodorization, and have covering effect to fishy smell.
Proteinase activated dose of composition and its parts by weight are：9 parts of starch, 6 parts of konjaku flour, 17 parts of maltodextrin, maltitol
14 parts are with 4 parts of active protein peptide, the amino acid sequence of wherein active protein peptide
HSHACKLCVCVNCFCGTAKCTHCRVLHPGKLCVCVNCSK.Above-mentioned proteinase activated dose produces tension force effect to substrate, makes
Substrate is distorted, and promotes ES to enter activated state, and can improve the activity of pancreas chymotrypsin, is improved the enzymolysis efficiency of protease, is subtracted
Few pancreas chymotrypsin consumption simultaneously shortens enzymolysis time, reduces production cost.There is hydrogen between activated protein and pancreas chymotrypsin
Key is acted on, and can change the molecular conformation of pancreas chymotrypsin, and is cooperated with other compositions in proteinase activated dose, can effectively be carried
High protein enzymatic activity.
Routine operation in the operating procedure of the present invention is well known to those skilled in the art, herein without repeating.
Technical scheme is described in detail embodiment described above, it should be understood that it is described above only
For the specific embodiment of the present invention, it is not intended to limit the invention, all any modifications made in the spirit of the present invention,
Supplement or similar fashion replacement etc., should be included in the scope of the protection.
SEQUENCE LISTING
<110>Zhejiang Feng Yu marine organisms Products Co., Ltd
<120>A kind of production method for hydrolyzing fish soluble slurry protein powder
<130> 1
<160> 1
<170> PatentIn version 3.5
<210> 1
<211> 39
<212> PRT
<213>It is artificial synthesized
<400> 1
His Ser His Ala Cys Lys Leu Cys Val Cys Val Asn Cys Phe Cys Gly
1 5 10 15
Thr Ala Lys Cys Thr His Cys Arg Val Leu His Pro Gly Lys Leu Cys
20 25 30
Val Cys Val Asn Cys Ser Lys
35
Claims (10)
1. a kind of production method for hydrolyzing fish soluble slurry protein powder, it is characterised in that：Described hydrolysis includes pancreas chymotrypsin and wind
Taste protease stepwise discretization, uses infrared ray temperature control, and added while pancreas chymotrypsin enzymolysis is added in enzymolysis process
Proteinase activated dose, enzymolysis terminates rear dialysis, takes spray drying after filtered solution, activated carbon and alcohol deodorization to obtain albumen powder.
2. a kind of production method for hydrolyzing fish soluble slurry protein powder according to claim 1, it is characterised in that：Described fish is molten
Slurry adds water dilution, the molten slurry of fish before protease hydrolyzed is added：Water=1:20~40v/v.
3. a kind of production method for hydrolyzing fish soluble slurry protein powder according to claim 1, it is characterised in that：Described albumen
Zymoexciter composition and its parts by weight are：8 ~ 10 parts of starch, 5 ~ 10 parts of konjaku flour, 15 ~ 20 parts of maltodextrin, maltitol 10 ~
16 parts are with 3 ~ 6 parts of active protein peptide, the amino acid sequence of wherein active protein peptide
HSHACKLCVCVNCFCGTAKCTHCRVLHPGKLCVCVNCSK。
4. a kind of production method for hydrolyzing fish soluble slurry protein powder according to claim 1, it is characterised in that：Described pancreas is rotten
Protease addition is 0.6 ~ 2.0% of the molten volume of slurry of fish before dilution, and proteinase activated dose of addition is the molten slurry of fish before dilution
Long-pending 0.001 ~ 0.004%.
5. a kind of production method for hydrolyzing fish soluble slurry protein powder according to claim 1, it is characterised in that：Described pancreas is rotten
Protease hydrolyzed temperature is 48 ~ 60 DEG C, and pH is 7.5 ~ 9.0, and enzymolysis time is 8 ~ 15min.
6. a kind of production method for hydrolyzing fish soluble slurry protein powder according to claim 1, it is characterised in that：Described pancreas is rotten
Protease hydrolyzed is gone out 4 ~ 8min of enzyme after terminating using boiling water bath, adds flavor protease, and addition is the molten volume of slurry of fish before dilution
0.3 ~ 1.2%.
7. a kind of production method for hydrolyzing fish soluble slurry protein powder according to claim 1, it is characterised in that：Described local flavor
Protease hydrolyzed temperature is 48 ~ 60 DEG C, and pH is 5.6 ~ 6.7, and enzymolysis time is 8 ~ 15min.
8. a kind of production method for hydrolyzing fish soluble slurry protein powder according to claim 1, it is characterised in that：Described local flavor
Protease hydrolyzed goes out enzyme after terminating, and using 2000 ~ 5000Da half membrane dialyzing, takes filtered solution.
9. a kind of production method for hydrolyzing fish soluble slurry protein powder according to claim 1, it is characterised in that：Described activity
The addition of charcoal is 0.5 ~ 1.5%w/v of filtered solution, and temperature is 34 ~ 42 DEG C, and the decoloration deodorization time is 20 ~ 50min.
10. a kind of production method for hydrolyzing fish soluble slurry protein powder according to claim 1, it is characterised in that：Described wine
Smart addition is the final concentration of 4 ~ 8%v/v of alcohol in filtered solution, and temperature is 17 ~ 25 DEG C, and the deodorant time is 14 ~ 25min.
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| CN111493205A (en) * | 2020-04-28 | 2020-08-07 | 广东海洋大学 | Fish protein hydrolysate and preparation method thereof |
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