CN107258539A - A kind of method for controlling taipei fritillary bulb tissue cultures endophytic bacterial contamination - Google Patents
A kind of method for controlling taipei fritillary bulb tissue cultures endophytic bacterial contamination Download PDFInfo
- Publication number
- CN107258539A CN107258539A CN201710547593.7A CN201710547593A CN107258539A CN 107258539 A CN107258539 A CN 107258539A CN 201710547593 A CN201710547593 A CN 201710547593A CN 107258539 A CN107258539 A CN 107258539A
- Authority
- CN
- China
- Prior art keywords
- bacterial contamination
- fritillary bulb
- tissue cultures
- taipei fritillary
- endophytic bacterial
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The present invention relates to biological technical field, and in particular to a kind of method of control taipei fritillary bulb tissue cultures endophytic bacterial contamination.Control method of the present invention includes：S1, explant selection and pretreatment：Taipei fritillary bulb Fresh Bulbs are selected, are soaked in washing powder solution, are wiped, are rinsed, filter paper is blotted, standby；S2, explant are disinfected：Pretreated explant is immersed in mercuric chloride solution, stirring, in superclean bench, is rinsed, and is dried, standby；S3, Bulblet induction differentiation：The explant scale that S2 processing is obtained is broken into two with one's hands, is slit longitudinally into the wide fritters of 2~4mm, is inoculated on the culture medium that with the addition of penicillin and carbendazim and is cultivated；S4, squamous subculture：Every 20 days squamous subcultures are once；S5 clove Multiplying cultures：The healthy clove that S4 is obtained, is transferred on the culture medium without any bactericidal agent and cultivates.The method of present invention control taipei fritillary bulb tissue cultures endophytic bacterial contamination, endophytic bacterial contamination control rate can reach 96.5%.
Description
Technical field
The present invention relates to biological technical field, and in particular to endophyte is dirty in one kind control taipei fritillary bulb tissue culture procedures
The method of dye.
Background technology
《Pharmacopoeia of People's Republic of China》The bulbus fritillariae cirrhosae recorded is liliaceous plant bulbus fritillariae cirrhosae Fritillaria
Cirrhosa D.Don, Fritillaria unibracteata Fritillaria unibracteata Hsiao et K.C.Hsia, Gansu fritillaria
Fritillaria przewalskii Maxim. Bulbus Fritillariae cirrhosae Fritillaria delavayi Franch. taipei fritillary bulbs
Fritillaria taipaiensis P.Y.Li or watt cloth fritillaria Fritillaria unibracteata Hsiao et
K.C.Hsia var wabuensis (Y.Tang et S.C.Yue) Z.D.Liu, S.C.Chen dry bulb.It is rare river
Birth canal ground one of bulk medicinal materials, are mainly used in lung-heat type cough, the few phlegm of dry cough, deficiency of Yin overstrain cough coughs up the illnesss such as sputum streaked with blood, clinical demand
Amount is huge.
Taipei fritillary bulb is included in 2010 editions as newly-increased source《Chinese Pharmacopoeia》, with wild resource is few, strong adaptability, low sea
Pull out also can the characteristic such as normal growth and development, be the optimal kind that cultivation is planted by suitable family in bulbus fritillariae cirrhosae.Taipei fritillary bulb planting industry welcomes
New opportunity to develop.
And existing bulbus fritillariae cirrhosae yield wretched insufficiency, demand but increases year by year, there are serious disparities between supply and demand.Its medicinal material comes
There are 4 kinds to be included in state three level protecting plant register in source and plantation is difficult.Biomass accumulation is slow within its growth period, plant
The death rate is high, so as to cause low in economic efficiency, peasant planting enthusiasm is not high, and this is always the bottle for restricting planting bulbus fritilariae development
Neck.
《CHINA JOURNAL OF CHINESE MATERIA MEDICA》Interim record within 1996 01《The quick reproduction test of taipei fritillary bulb》, text in report
Taipei fritillary bulb tissue-culturing quick-propagation is tested.Research shows：Using MS+NAA1.0mg/L+6~BA3.0mg/L culture mediums, lure
Lead callus and bulb bearing frequency is 93% again；In callus and regeneration bulb containing 15 kinds of constants needed by human and
The content of trace element, wherein most elements is significantly more than cultivation bulb.But cultivated in the prior art by the method
The probability that obtained bulb infects endophyte in cultivating process is very big, and the ratio that can be smoothed out turning generation continuation culture is low.
Therefore, it is very crucial to the pollution for controlling taipei fritillary bulb tissue culture endophyte.
The content of the invention
Taipei fritillary bulb tissue cultures endophyte is controlled it is an object of the invention to overcome the deficiencies of the prior art and provide one kind
The high control method of pollution rate.
The control method of endophyte, comprises the following steps in taipei fritillary bulb tissue culture procedures of the present invention：
S1, explant selection and pretreatment：The taipei fritillary bulb Fresh Bulbs taken using 3~April remove outer layer as explant
The rotten, scale of browning, cuts off root system；Clean and 4~6min is soaked in bulb surface, washing powder solution, wipe bulb surface, stream
Water rinses 0.8~1.2h, then with deionized water rinsing 4~6 times, filter paper is blotted, standby；
S2, explant are disinfected：Explant after S1 is handled, is immersed in the mercury chloride that concentration is 0.08~0.12%
In solution, stirring in superclean bench, is rinsed, dried, standby；
S3, Bulblet induction differentiation：The aseptic explant that S2 is obtained, is broken into two with one's hands its scale with sterilized tweezers, then
The wide strip fritters of 2~4mm are obtained with sterile razor blade rip cutting scale, fritter is inoculated in the culture that with the addition of penicillin and carbendazim
Cultivated on base；
S4, clove squamous subculture：By the clove of the induction differentiation culture of S3 steps in same medium, every 20 days
Squamous subculture once, is total to squamous subculture 3 times；
S5, clove Multiplying culture：The eugonic healthy clove that S4 is obtained, is transferred to without any sterilizing
85~95d of Multiplying culture on the culture medium of agent.
The method of above-mentioned control taipei fritillary bulb tissue cultures endophytic bacterial contamination, wherein washing powder solution is described in S1 steps
By washing powder and water according to 0.8~1.2g of w/v:1000mL configurations are formed.
Further, the method for above-mentioned control taipei fritillary bulb tissue cultures endophytic bacterial contamination, is wherein washed described in S1 steps
Clothing powder solution is according to w/v 1.0g by washing powder and water:1000mL configurations are formed.
The method of above-mentioned control taipei fritillary bulb tissue cultures endophytic bacterial contamination, wherein stirring uses magnetic force in the S2 steps
Agitator is stirred, and mixing time is 6~8min, and mixing speed is 150~200r/min.
The method of above-mentioned control taipei fritillary bulb tissue cultures endophytic bacterial contamination, wherein rinsing is with sterile in the S2 steps
Water is rinsed 3~5 times, and each rinsing time is 3~5min；Dry to be blotted with aseptic filter paper.
The method of above-mentioned control taipei fritillary bulb tissue cultures endophytic bacterial contamination, wherein cultivation temperature is 18 in the S3 steps
~22 DEG C, relative humidity is 75~85%, and intensity of illumination is 1000~1500Lx, and light application time is 11~13h.
Further, the method for above-mentioned control taipei fritillary bulb tissue cultures endophytic bacterial contamination, wherein being trained in the S3 steps
It is 20 DEG C to support temperature, and relative humidity is 80%, and intensity of illumination is 1000~1500Lx, and light application time is 12h.
The method of above-mentioned control taipei fritillary bulb tissue cultures endophytic bacterial contamination, wherein the pH value of the culture medium be 5.6~
5.8；Be by+3.0~5.0mg/L of MS culture mediums+0.8~1.2mg/L of 6- benzyl aminoadenines α-naphthylacetic acid+4.0~
6.0mg/L penicillin+0.8~1.2g/L sucrose+9g/L of carbendazim+3% agar composition.
Further, the method for above-mentioned control taipei fritillary bulb tissue cultures endophytic bacterial contamination, wherein the inducing culture
It is the penicillin+1.0g/L of the methyl α-naphthyl acetate+5.0mg/L by MS culture mediums+4.0mg/L 6- benzyl aminoadenines+1.0mg/L
Sucrose+the 9g/L of carbendazim+3% agar is constituted.
The method of above-mentioned control taipei fritillary bulb tissue cultures endophytic bacterial contamination, wherein culture medium is by MS described in S5 steps
Minimal medium+3.0~5.0mg/L+0.8~1.2mg/L of the 6- benzyl aminoadenines sucrose+9g/L agar of methyl α-naphthyl acetate+3%
Composition.
The beneficial effects of the invention are as follows：
1st, taipei fritillary bulb tissue cultures endophytic bacterial contamination is controlled, contamination control rate reaches 96.5%, and explant usage amount subtracts
It is few, reduce production cost；
2nd, taipei fritillary bulb tissue cultures endophytic bacterial contamination is controlled, direct induced synthesis clove is more, peasant household, enterprise is produced
Introduces a collection is in short supply is of great importance for plantation；
3rd, pollution rate is controlled, and bulb flourish is fast, and a large amount of bulb medicinal materials are provided for market, alleviates imbalance between supply and demand.
Embodiment
Technical scheme is described in further detail with reference to specific embodiment, but protection scope of the present invention is not
It is confined to as described below.
Embodiment 1
1st, explant selection and pretreatment：
Excavation 3~April taipei fritillary bulb Fresh Bulbs make explant, remove outer layer rot, the scale of browning, cut off root
System；Bulb surface soil is cleaned, 5min is soaked in concentration is 1g/L washing powder solution, is flowed with after brush gently brushing surface
Water rinses 1h, then is rinsed 5 times with aseptic deionized water, and filter paper is blotted, standby；
2nd, explant is disinfected：Explant after processing is inserted in wide-mouth bottle, under normal temperature, concentration 0.1% is poured into
Mercuric chloride solution is to flooding explant.With 6~8min of magnetic stirring apparatus stirring at low speed explant, 75% alcohol wipe wide-mouth bottle table
It is put into behind face in superclean bench, under aseptic condition, with rinsed with sterile water bulb 3~5 times, 3~5min every time, finally with sterilizing
Filter paper is blotted, standby；
3rd, the induction differentiation of clove：Under aseptic condition, taipei fritillary bulb scale is broken into two with one's hands with tweezers, with sterile razor blade by meat
Matter scale is slit longitudinally into the wide fritters of about 3mm, is inoculated on Bulblet induction culture medium, and the culture medium is：MS minimal mediums+
6-BA (6- benzyls aminoadenine) 4.0mg/L+NAA (α-naphthylacetic acid) 1.0mg/L+ penicillin 5.0mg/L+ carbendazim 1.0g/L+
Sucrose 3%+9g/L agar 1.0%, pH is 5.6~5.8.After inoculation, 20 DEG C of temperature is placed in, relative humidity 80%, illumination 12h,
Intensity be 1000~1500Lx under the conditions of cultivated；
4th, squamous subculture：In same medium, every 20 days squamous subcultures are carried out once to the clove after induction differentiation,
It is total to squamous subculture 3 times；
5th, clove Multiplying culture：Aging and undergrowth bulb is removed, vigorous healthy small of growth selection
Bulb, cuts, and removes base portion residue, is transferred to culture medium MS+6-BA (6- benzyls aminoadenine) 4.0mg/L+NAA (α-naphthalene
Acetic acid) 1.0mg/L+ sucrose 3%+9g/L agar 1.0%, pH is statistics after 5.6~5.8 progress shoot proliferation cultures, culture 90d
Clove survival rate.
In order to reduce endophytic bacterial contamination during taipei fritillary bulb bulb tissue culture, the proliferation rate and survival rate of bulb are improved.Examination
Test design and two kinds of bactericidal agents of carbendazim and penicillin are added in culture medium MS+6-BA4.0mg/L+NAA1.0mg/L, in it
Raw bacterium pollution is controlled.Concentration is shown in Table 1.Two factor completely randoms, each processing is shown in Table 2.
With culture medium：MS+6-BA 4.0mg/L+NAA 1.0mg/L are control.Under aseptic condition, with sterile razor blade by meat
Matter scale is slit longitudinally into the wide fritters of about 3mm, is inoculated in each processing culture medium, 5 repetitions/processing, 5 bottles/repeat, and 3 pieces of bulbs/
Bottle.Other conditions are consistent.Directly remove, do not counted if any pollution scale in 7d.After 7d, endophytic bacterial contamination situation is monitored daily,
As polluted, untainted scale is shifted immediately, and pollution scale continues to observe.And observed and recorded Bulblet induction differentiation situation.60d
Count endophytic bacterial contamination rate.
The experimental factor of table 1 and concentration
Each processing culture medium that endophyte grows during the control taipei fritillary bulb bulb tissue culture of table 2
| Processing | Culture medium |
| 1 | MS+6-BA4.0mg/L+NAA1.0mg/L+ penicillin 5.0mg/L+ carbendazim 0.5g/L |
| 2 | MS+6-BA4.0mg/L+NAA1.0mg/L+ penicillin 5.0mg/L+ carbendazim 1.0g/L |
| 3 | MS+6-BA4.0mg/L+NAA1.0mg/L+ penicillin 5.0mg/L+ carbendazim 1.5g/L |
| 4 | MS+6-BA4.0mg/L+NAA1.0mg/L+ penicillin 10.0mg/L+ carbendazim 0.5g/L |
| 5 | MS+6-BA4.0mg/L+NAA1.0mg/L+ penicillin 10.0mg/L+ carbendazim 1.0g/L |
| 6 | MS+6-BA4.0mg/L+NAA1.0mg/L+ penicillin 10.0mg/L+ carbendazim 1.5g/L |
| 7 | MS+6-BA4.0mg/L+NAA1.0mg/L+ penicillin 20.0mg/L+ carbendazim 0.5g/L |
| 8 | MS+6-BA4.0mg/L+NAA1.0mg/L+ penicillin 20.0mg/L+ carbendazim 1.0g/L |
| 9 | MS+6-BA4.0mg/L+NAA1.0mg/L+ penicillin 20.0mg/L+ carbendazim 1.5g/L |
| CK | MS+6-BA4.0mg/L+NAA1.0mg/L |
The taipei fritillary bulb bulb tissue culture endophytic bacterial contamination application form of table 3
Note：
As a result：
1st, the no culture medium (CK) of addition penicillin and carbendazim, endophytic bacterial contamination is serious, and pollution rate is up to 72.3%；
2nd, penicillin and carbendazim concentration are too low (processing 1), and pollution rate is higher (23.2%), is differentiated to form clove, after
Phase growth is preferable；
3rd, increase with penicillin and carbendazim concentration, endophytic bacterial contamination reduction, but scale is undifferentiated does not induce small squama
Stem.
Integrated comparative, 2 penicillin of processing and carbendazim concentration proportioning are most suitable, and pollution rate is low (2.5%), scale differentiation shape
Into clove it is many, and grow it is vigorous.Compared with the control, endophytic bacterial contamination control rate reaches 96.5%.
Described above is only the preferred embodiment of the present invention, it should be understood that the present invention is not limited to described herein
Form, is not to be taken as the exclusion to other embodiment, and available for various other combinations, modification and environment, and can be at this
In the text contemplated scope, it is modified by the technology or knowledge of above-mentioned teaching or association area.And those skilled in the art are entered
Capable change and change does not depart from the spirit and scope of the present invention, then all should appended claims of the present invention protection domain
It is interior.
Claims (10)
1. control the method for taipei fritillary bulb tissue cultures endophytic bacterial contamination, it is characterised in that comprise the following steps：
S1, explant selection and pretreatment：The taipei fritillary bulb Fresh Bulbs taken using 3~April remove outer layer rotten as explant
Rotten, browning scale, cuts off root system；Clean and 4~6min is soaked in bulb surface, washing powder solution, wipe bulb surface, flowing water
Rinse 0.8~1.2h, then with deionized water rinsing 4~6 times, filter paper is blotted, standby；
S2, explant are disinfected：Explant after S1 is handled, is immersed in the mercuric chloride solution that concentration is 0.08~0.12%
In, stirring in superclean bench, is rinsed, dried, standby；
S3, Bulblet induction differentiation：The aseptic explant that S2 is obtained, is broken into two with one's hands its scale with sterilized tweezers, then with nothing
Bacterium blade rip cutting scale obtains the wide strip fritters of 2~4mm, and fritter is inoculated on culture medium and cultivated；
S4, squamous subculture：By the clove of the culture of S3 steps in same medium, every 20 days squamous subcultures once, altogether after
It is commissioned to train foster 3 times；
S5, clove Multiplying culture：The eugonic healthy clove that S4 is obtained, is transferred to without any bactericidal agent
85~95d of Multiplying culture on culture medium.
2. the method for taipei fritillary bulb tissue cultures endophytic bacterial contamination is controlled according to claim 1, it is characterised in that S1 steps
Described in washing powder solution be according to 0.8~1.2g of w/v by washing powder and water:1000mL configurations are formed.
3. the method for taipei fritillary bulb tissue cultures endophytic bacterial contamination is controlled according to claim 2, it is characterised in that S1 steps
Described in washing powder solution be according to w/v 1.0g by washing powder and water:1000mL configurations are formed.
4. the method for taipei fritillary bulb tissue cultures endophytic bacterial contamination is controlled according to claim 1, it is characterised in that the S2
Stirring uses magnetic stirrer in step, and mixing time is 6~8min, and mixing speed is 150~200r/min.
5. the method for taipei fritillary bulb tissue cultures endophytic bacterial contamination is controlled according to claim 1, it is characterised in that the S2
Rinsing is to use rinsed with sterile water 3~5 times in step, and each rinsing time is 3~5min；Dry to be blotted with aseptic filter paper.
6. the method for taipei fritillary bulb tissue cultures endophytic bacterial contamination is controlled according to claim 1, it is characterised in that the S3
Cultivation temperature is 18~22 DEG C in step, and relative humidity is 75~85%, and intensity of illumination is 1000~1500Lx, and light application time is
11~13h.
7. the method for taipei fritillary bulb tissue cultures endophytic bacterial contamination is controlled according to claim 6, it is characterised in that the S3
Cultivation temperature is 20 DEG C in step, and relative humidity is 80%, and intensity of illumination is 1000~1500Lx, and light application time is 12h.
8. the method for taipei fritillary bulb tissue cultures endophytic bacterial contamination is controlled according to claim 1, it is characterised in that S3, S4
The pH value of culture medium described in step is 5.6~5.8；It is fast by+3.0~5.0mg/L of MS minimal mediums 6- benzyl amino glands
Purine+0.8~1.2mg/L α-naphthylacetic acid+4.0~6.0mg/L penicillin+0.8~1.2g/L sucrose of carbendazim+3%+9g/
L agar is constituted.
9. the method for control taipei fritillary bulb tissue cultures endophytic bacterial contamination according to claim 8, it is characterised in that described
Inducing culture is by MS minimal mediums+4.0mg/L 6- benzyl aminoadenines+1.0mg/L α-naphthylacetic acid+5.0mg/L
Penicillin+1.0g/L the sucrose+9g/L of carbendazim+3% agar composition.
10. the method for taipei fritillary bulb tissue cultures endophytic bacterial contamination is controlled according to claim 1, it is characterised in that S5 is walked
Culture medium described in rapid be by+3.0~5.0mg/L of MS minimal mediums+0.8~1.2mg/L of 6- benzyl aminoadenines α-
Sucrose+the 9g/L of methyl α-naphthyl acetate+3% agar is constituted.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710547593.7A CN107258539B (en) | 2017-07-06 | 2017-07-06 | A method of control taipei fritillary bulb tissue cultures endophytic bacterial contamination |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710547593.7A CN107258539B (en) | 2017-07-06 | 2017-07-06 | A method of control taipei fritillary bulb tissue cultures endophytic bacterial contamination |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107258539A true CN107258539A (en) | 2017-10-20 |
| CN107258539B CN107258539B (en) | 2019-06-07 |
Family
ID=60073226
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710547593.7A Active CN107258539B (en) | 2017-07-06 | 2017-07-06 | A method of control taipei fritillary bulb tissue cultures endophytic bacterial contamination |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107258539B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111296211A (en) * | 2020-03-12 | 2020-06-19 | 重庆市药物种植研究所 | Rapid propagation method of fritillaria taipaiensis bulbs |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102172223A (en) * | 2011-03-24 | 2011-09-07 | 浙江森禾种业股份有限公司 | Method for removing endotrophic bacteria in tissue culture system of nandina with red leaves |
| CN103238525A (en) * | 2013-05-31 | 2013-08-14 | 常熟市佳盛农业科技发展有限公司 | Method for breeding fritillary bulb by tissue culture technique |
| CN103283604A (en) * | 2013-06-07 | 2013-09-11 | 南京工业大学大丰海洋产业研究院 | Method for removing endophytic bacteria of explants in lily tissue culture |
| CN103444528A (en) * | 2013-08-16 | 2013-12-18 | 苏州仁成生物科技有限公司 | Growth promotion cultivation method of fritillary bulbs |
| CN104782499A (en) * | 2015-05-11 | 2015-07-22 | 南阳师范学院 | Method for multiplying vetiver grass seedlings from stem auxiliary buds |
| CN105706927A (en) * | 2016-02-04 | 2016-06-29 | 广西壮族自治区林业科学研究院 | Open rooting culture method for 4-terpilenol melaleuca alternefolia tissue culture subculture buds |
-
2017
- 2017-07-06 CN CN201710547593.7A patent/CN107258539B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102172223A (en) * | 2011-03-24 | 2011-09-07 | 浙江森禾种业股份有限公司 | Method for removing endotrophic bacteria in tissue culture system of nandina with red leaves |
| CN103238525A (en) * | 2013-05-31 | 2013-08-14 | 常熟市佳盛农业科技发展有限公司 | Method for breeding fritillary bulb by tissue culture technique |
| CN103283604A (en) * | 2013-06-07 | 2013-09-11 | 南京工业大学大丰海洋产业研究院 | Method for removing endophytic bacteria of explants in lily tissue culture |
| CN103444528A (en) * | 2013-08-16 | 2013-12-18 | 苏州仁成生物科技有限公司 | Growth promotion cultivation method of fritillary bulbs |
| CN104782499A (en) * | 2015-05-11 | 2015-07-22 | 南阳师范学院 | Method for multiplying vetiver grass seedlings from stem auxiliary buds |
| CN105706927A (en) * | 2016-02-04 | 2016-06-29 | 广西壮族自治区林业科学研究院 | Open rooting culture method for 4-terpilenol melaleuca alternefolia tissue culture subculture buds |
Non-Patent Citations (3)
| Title |
|---|
| 刘玉红 等: "太白贝母的快速繁殖试验", 《中国中药杂志》 * |
| 林顺权: "《园艺植物生物技术》", 31 August 2007, 中国农业出版社 * |
| 贾晗 等: "太白贝母不同器官愈伤组织诱导培养研究", 《亚太传统医药》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111296211A (en) * | 2020-03-12 | 2020-06-19 | 重庆市药物种植研究所 | Rapid propagation method of fritillaria taipaiensis bulbs |
Also Published As
| Publication number | Publication date |
|---|---|
| CN107258539B (en) | 2019-06-07 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107347651A (en) | The cultural method that dove tree hibernaculum quickly breeds | |
| CN106508460B (en) | Method for rapidly identifying broomrape resistance level of sunflower indoors | |
| CN103461134A (en) | Method for cultivating water lily aseptic seedling | |
| CN101999317B (en) | Multiploid bulb induction method with firtillaria cirrhosa scale leaf as explant | |
| CN105961205B (en) | A kind of method for tissue culture for improving pearl guava survival rate | |
| CN101116424B (en) | Highly effective lily bulblet inducement culture method | |
| CN104855285A (en) | One-time seedling induction method for organic cultivation of polyploid Anoectochilus roxburghii | |
| CN103173365B (en) | Method for indoor culture breeding of Plasmopara viticola | |
| CN108633376A (en) | A kind of cultural method of the sub- non-symbiosis germination of Gree pocket orchid species | |
| CN103270950A (en) | Chrysanthemum simplified tissue culturing method | |
| CN104719168B (en) | The method for cultivating bletilla striata seedling using interval submergence bioreactor | |
| CN110249996A (en) | It is a kind of reuse shell substrate method and seaweed shell conchocelis breeding method | |
| CN117063780B (en) | Cordyceps sinensis ascospore harvesting culture medium and harvesting method thereof | |
| CN104823849A (en) | Rapid propagation method of cassava virus-free seedlings | |
| CN107258539B (en) | A method of control taipei fritillary bulb tissue cultures endophytic bacterial contamination | |
| CN108142281A (en) | A kind of Cortex Eucommiae method for tissue culture | |
| CN105918132A (en) | Rapid breeding method of clerodendrum trichotomum thunb | |
| CN103283604B (en) | Method for removing endophytic bacteria of explants in lily tissue culture | |
| CN108260529A (en) | A kind of tissue cultivating and seedling method of red root wild silkworm beans | |
| CN107372120A (en) | The cultural method that dove tree branch is quickly bred | |
| CN101390494B (en) | New method for obtaining ornamental arrowroot tissue culture sterile system | |
| CN107821169A (en) | A kind of tissue culture method of P. kingianum seedling | |
| CN107372117A (en) | Taxus chinensis var. mairei Callus abductive approach | |
| CN108812327B (en) | Culture solution and culture method for water culture propagation of chrysosporium | |
| CN107616093B (en) | Tissue culture rapid propagation method of Ardisia macrophylla |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |