CN107258154A - A kind of method that tissue culture is sterilized with soya seeds - Google Patents
A kind of method that tissue culture is sterilized with soya seeds Download PDFInfo
- Publication number
- CN107258154A CN107258154A CN201710677421.1A CN201710677421A CN107258154A CN 107258154 A CN107258154 A CN 107258154A CN 201710677421 A CN201710677421 A CN 201710677421A CN 107258154 A CN107258154 A CN 107258154A
- Authority
- CN
- China
- Prior art keywords
- sterilized
- soya seeds
- tissue culture
- sulfuric acid
- seeds
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/08—Immunising seed
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
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- Life Sciences & Earth Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Soil Sciences (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The invention discloses a kind of method that tissue culture is sterilized with soya seeds, first with 70% ethanol postincubation 1min, then with after 0.2% sulfuric acid treatment 5min, 1% hypochlorite disinfectant 15min, with aseptic water washing 4~5 times, 17~20h is finally soaked in sterilized water.The present invention utilizes 0.2% sulfuric acid treatment soya seeds, can effectively break the phenomenon of its kind of skin bad hydraulic permeability, improves soya seeds and exempts from contaminated ability.
Description
Technical field
The present invention relates to biological technical field, and in particular to the modification method that a kind of tissue culture is sterilized with soya seeds.
Background technology
Soybean is a kind of important industrial crops.Recently as the development of plant transgenic technology, the tissue of soybean is trained
Support and obtained extensive research.The explant commonly used in soyabean tissue cultivates has cotyledonary node and plumule point etc., therefore obtains nothing
The explant of bacterium infection is that soybean tissue culture is successfully crucial.But, because the water permeable ability of soybean peel is poor and different product
The water penetration of inter-species kind skin has differences, therefore easily generation soya seeds are not thorough in disinfecting process, and explant is easily dirty
Dye, causes tissue culture to fail.
The content of the invention
In view of the shortcomings of the prior art, the present invention is intended to provide the modification method that a kind of tissue culture is sterilized with soya seeds, profit
With 0.2% sulfuric acid treatment soya seeds, can effectively break the phenomenon of its kind of skin bad hydraulic permeability, improve soya seeds from
The ability of pollution.
To achieve these goals, the present invention is adopted the following technical scheme that：
The modification method that a kind of tissue culture is sterilized with soya seeds, comprises the following steps：
Tissue culture is first sterilized 1min by S1 with 70% ethanol postincubation of soya seeds；
S2 soaks 5min with 0.2% sulfuric acid, then with 1% hypochlorite disinfectant 15min；
S3 aseptic water washings, finally soak in sterilized water.
Further, in step S3, with aseptic water washing 4~5 times, 17~20h is finally soaked in sterilized water.
The beneficial effects of the present invention are：The present invention sets in the sterilization to tissue culture soya seeds and utilizes 0.2%
The process step of sulfuric acid, can effectively break the phenomenon of its kind of skin bad hydraulic permeability, improve soya seeds and exempt from contaminated energy
Power.
Embodiment
The invention will be further described below, it is necessary to which explanation, following examples are using the technical program before
Carry, give detailed embodiment and specific operating process, but protection scope of the present invention is not limited to the present embodiment.
The modification method that a kind of tissue culture is sterilized with soya seeds, comprises the following steps：
Tissue culture is first sterilized 1min by S1 with 70% ethanol postincubation of soya seeds；
S2 soaks 5min with 0.2% sulfuric acid, then with 1% hypochlorite disinfectant 15min；
S3 aseptic water washings, finally soak in sterilized water.
Further, in step S3, with aseptic water washing 4~5 times, 17~20h is finally soaked in sterilized water.
In order to further illustrate the technique effect of the inventive method, comparative experimental data is now provided as shown in table 1：
Table 1
Wherein, the molecule in each numerical value is the soya seeds number of pollution, and denominator is the soya seeds sum sprouted.Can
To find out, tissue culture is carried out by the inventive method and sterilized with soya seeds, can effectively break showing for its kind of skin bad hydraulic permeability
As improving soya seeds and exempting from contaminated ability.
For those skilled in the art, technical scheme that can be more than and design, provide various corresponding
Change and deform, and all these change and deform, and should be construed as being included within the protection domain of the claims in the present invention.
Claims (2)
1. the modification method that a kind of tissue culture is sterilized with soya seeds, it is characterised in that comprise the following steps：
Tissue culture is first sterilized 1min by S1 with 70% ethanol postincubation of soya seeds；
S2 soaks 5min with 0.2% sulfuric acid, then with 1% hypochlorite disinfectant 15min；
S3 aseptic water washings, finally soak in sterilized water.
2. the modification method that tissue culture according to claim 1 is sterilized with soya seeds, it is characterised in that in step S3, is used
Aseptic water washing 4~5 times, finally soaks 17~20h in sterilized water.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710677421.1A CN107258154A (en) | 2017-08-09 | 2017-08-09 | A kind of method that tissue culture is sterilized with soya seeds |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710677421.1A CN107258154A (en) | 2017-08-09 | 2017-08-09 | A kind of method that tissue culture is sterilized with soya seeds |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107258154A true CN107258154A (en) | 2017-10-20 |
Family
ID=60076844
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710677421.1A Pending CN107258154A (en) | 2017-08-09 | 2017-08-09 | A kind of method that tissue culture is sterilized with soya seeds |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107258154A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110073976A (en) * | 2019-05-07 | 2019-08-02 | 山西省农业科学院棉花研究所 | A kind of sterilization method of tissue culture soya seeds |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101451119A (en) * | 2008-12-26 | 2009-06-10 | 南京师范大学 | Phytolacca americana seed quick-speed germination method and its application in aseptic seedlings culture |
| CN102870671A (en) * | 2011-07-11 | 2013-01-16 | 中国科学院植物研究所 | High-efficiency aseptic seedling acquisition method from grass seed |
| CN103329659A (en) * | 2013-06-06 | 2013-10-02 | 商丘师范学院 | Method for improving germination rate and survival rate of sophora alopecuroide seeds and sophora alopecuroide seeds |
| CN104737668A (en) * | 2015-03-24 | 2015-07-01 | 商丘师范学院 | Wild soybean seeds and method for increasing germination rate and survival rate thereof |
-
2017
- 2017-08-09 CN CN201710677421.1A patent/CN107258154A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101451119A (en) * | 2008-12-26 | 2009-06-10 | 南京师范大学 | Phytolacca americana seed quick-speed germination method and its application in aseptic seedlings culture |
| CN102870671A (en) * | 2011-07-11 | 2013-01-16 | 中国科学院植物研究所 | High-efficiency aseptic seedling acquisition method from grass seed |
| CN103329659A (en) * | 2013-06-06 | 2013-10-02 | 商丘师范学院 | Method for improving germination rate and survival rate of sophora alopecuroide seeds and sophora alopecuroide seeds |
| CN104737668A (en) * | 2015-03-24 | 2015-07-01 | 商丘师范学院 | Wild soybean seeds and method for increasing germination rate and survival rate thereof |
Non-Patent Citations (1)
| Title |
|---|
| 华春等: "不同消毒剂对毕氏海蓬种子萌发的影响", 《安徽农学通报》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110073976A (en) * | 2019-05-07 | 2019-08-02 | 山西省农业科学院棉花研究所 | A kind of sterilization method of tissue culture soya seeds |
| CN110073976B (en) * | 2019-05-07 | 2022-08-19 | 山西省农业科学院棉花研究所 | Method for sterilizing tissue culture soybean seeds |
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| PB01 | Publication | ||
| PB01 | Publication | ||
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| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20171020 |