CN104106466A - Explant disinfection method in plant tissue culture - Google Patents
Explant disinfection method in plant tissue culture Download PDFInfo
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- CN104106466A CN104106466A CN201410262451.2A CN201410262451A CN104106466A CN 104106466 A CN104106466 A CN 104106466A CN 201410262451 A CN201410262451 A CN 201410262451A CN 104106466 A CN104106466 A CN 104106466A
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Abstract
An explant disinfection method in plant tissue culture is disclosed. A material is pretreated by a liquid detergent and penicillin, and then is treated by a disinfectant. The method has characteristics of easy disinfection, good disinfection effects, low toxicity, difficult damaging for the material, and environment protection.
Description
Technical field
The present invention relates to field of plant tissue culture technique, relate in particular to the method for explant sterilization in a kind of Plant Tissue Breeding.
Background technology
The explant major part that Plant Tissue Breeding is used is taken from field, adheres to a large amount of microorganisms on surface, and this is the large obstacle that tissue is cultivated.Therefore before material inoculated and cultured, must disinfect, sterilization requires on the one hand the various microorganism killings on material surface, can not damage again or slight damage organization material and do not affect its growth only simultaneously.Therefore, disinfecting of explant is the important ring in Plant Tissue Breeding work.
Existing sterilization method, Disinfection Effect is bad, and the microorganism on material can not be killed to greatest extent, and, existing sterilization method, explant is vulnerable to infringement, greatly reduces the survival rate of Plant Tissue Breeding.
Summary of the invention
The object of this part is to summarize some aspects of embodiments of the invention and briefly introduces some preferred embodiments.In this part and the application's specification digest and denomination of invention, may do a little simplification or omit to avoid the making object of this part, specification digest and denomination of invention fuzzy, and this simplification or omit can not be for limiting the scope of the invention.
Problem in view of existing in explant sterilization method in above-mentioned and/or existing Plant Tissue Breeding, has proposed the present invention.
Therefore, the present invention wherein object is to provide the method for the sterilization of explant in a kind of Plant Tissue Breeding, this sterilization method, and its sterilization is easily, Disinfection Effect is good, and toxicity is little, easy damaged material not, environmental protection.
For solving the problems of the technologies described above, according to an aspect of the present invention, the invention provides following technical scheme: a kind of method of explant sterilization in Plant Tissue Breeding, use liquid detergent and penicillin to pretreatment, and then adopt disinfectant to process material.
As preferred embodiment of the present invention, described thimerosal is alcohol and liquor natrii hypochloritis.
As preferred embodiment of the present invention, it specifically comprises the steps:
Step 1: adopt liquid detergent solution soak-out material 5～15min of 0.1～0.5%, running water rinses 20～40min;
Step 2: adopt the running water that contains 1～5% penicillin to soak 4～8min, running water rinses 5～10min;
Step 3: step 1 and the pretreated material of step 2 are adopted to alcohol-pickled 10～30s of 70～80%, sterile water wash 2～4 times;
Step 4: adopt 5～10% clorox to soak 5～20min, sterile water wash 4～7 times.
As preferred embodiment of the present invention, in described step 1, adopt 0.3% liquid detergent solution soak-out material 10min.
As preferred embodiment of the present invention, in described step 1, adopt running water to rinse 30min.
As preferred embodiment of the present invention, in described step 2, adopt the running water that contains 3% penicillin to soak 6min, running water rinses 7min.
As preferred embodiment of the present invention, step 3: adopt 75% alcohol-pickled material 10s, sterile water wash 3 times.
As preferred embodiment of the present invention, adopt 7% clorox to soak 10min, sterile water wash 6 times.
The method that the invention provides the sterilization of explant in a kind of Plant Tissue Breeding, its sterilization is easy, bactericidal effect good, and toxicity is little, easy damaged material not, environmental protection.
Embodiment
For above-mentioned purpose of the present invention, feature and advantage can be become apparent more, below the specific embodiment of the present invention is described in detail.
A lot of details have been set forth in the following description so that fully understand the present invention, but the present invention can also adopt other to be different from alternate manner described here and implement, those skilled in the art can do similar popularization without prejudice to intension of the present invention in the situation that, so the present invention is not subject to the restriction of following public specific embodiment.
The invention provides the method for explant sterilization in a kind of Plant Tissue Breeding, use liquid detergent and penicillin to pretreatment, and then adopt disinfectant to process material.
Below adopt embodiment to describe embodiments of the present invention in detail, to the present invention, how application technology means solve technical problem whereby, and the implementation procedure of reaching technique effect can fully understand and implement according to this.
Embodiment 1
Explant material is cut into segment or fritter or sheet or thread, then carries out disinfection according to the following steps:
Step 1: adopt 0.1% liquid detergent solution soak-out material 15min, running water rinses 20min;
Step 2: adopt the running water that contains 1% penicillin to soak 8min, running water rinses 5min, completes pretreatment;
Step 3: step 1 and the pretreated material of step 2 are adopted to 70% alcohol-pickled 30s, sterile water wash 2 times;
Step 4: adopt 5% clorox to soak 20min, sterile water wash 4 times, completes sterilization.
Embodiment 2
Explant material is cut into segment or fritter or sheet or thread, then carries out disinfection according to the following steps:
Step 1: adopt 0.2% liquid detergent solution soak-out material 15min, running water rinses 40min;
Step 2: adopt the running water that contains 2% penicillin to soak 8min, running water rinses 10min;
Step 3: step 1 and the pretreated material of step 2 are adopted to 70% alcohol-pickled 30s, sterile water wash 4 times;
Step 4: adopt 6% clorox to soak 20min, sterile water wash 7 times, completes sterilization.
Embodiment 3
Explant material is cut into segment or fritter or sheet or thread, then carries out disinfection according to the following steps:
Step 1: adopt 0.3% liquid detergent solution soak-out material 6min, running water rinses 30min;
Step 2: adopt the running water that contains 3% penicillin to soak 6min, running water rinses 7min;
Step 3: step 1 and the pretreated material of step 2 are adopted to 75% alcohol-pickled 10s, sterile water wash 3 times;
Step 4: adopt 7% clorox to soak 10min, sterile water wash 6 times, completes sterilization.
Embodiment 4
Explant material is cut into segment or fritter or sheet or thread, then carries out disinfection according to the following steps:
Step 1: adopt 0.5% liquid detergent solution soak-out material 5min, running water rinses 40min;
Step 2: adopt the running water that contains 5% penicillin to soak 4min, running water rinses 10min;
Step 3: step 1 and the pretreated material of step 2 are adopted to 80% alcohol-pickled 10s, sterile water wash 4;
Step 4: adopt 10% clorox to soak 5min, sterile water wash 7 times, completes sterilization.
Sterilization effect
Select the sterilization method of the embodiment of the present invention 1～4 and traditional sterilizing methods to organize culture experiment to explant, its aseptic rate contrast table is in Table 1:
Table 1 sterilization method of the present invention and the traditional method for disinfection killing effect to bacterium and fungi
| Sequence number | Sterilization complexity | Aseptic rate (%) |
| Embodiment 1 | Easily | 98 |
| Embodiment 2 | Easily | 99 |
| Embodiment 3 | The easiest | 100 |
| Embodiment 4 | The easiest | 100 |
| Tradition method for disinfection | Be difficult for | 20.1 |
It should be noted that, the present invention does not limit the kind of explant and position.
As can be seen here, the method for explant sterilization in Plant Tissue Breeding of the present invention, its sterilization is easy, bactericidal effect good, and toxicity is little, easy damaged material not, environmental protection.
It should be noted that, above embodiment is only unrestricted in order to technical scheme of the present invention to be described, although the present invention is had been described in detail with reference to preferred embodiment, those of ordinary skill in the art is to be understood that, can modify or be equal to replacement technical scheme of the present invention, and not departing from the spirit and scope of technical solution of the present invention, it all should be encompassed in the middle of claim scope of the present invention.
Claims (8)
1. a method for explant sterilization in Plant Tissue Breeding, is characterized in that: use liquid detergent and penicillin to pretreatment, and then adopt disinfectant to process material.
2. the method for explant sterilization in Plant Tissue Breeding according to claim 1, is characterized in that: described thimerosal is alcohol and liquor natrii hypochloritis.
3. the method that in Plant Tissue Breeding according to claim 2, explant is sterilized, is characterized in that:
It specifically comprises the steps:
Step 1: adopt liquid detergent solution soak-out material 5～15min of 0.1～0.5%, running water rinses 20～40min;
Step 2: adopt the running water that contains 1～5% penicillin to soak 4～8min, running water rinses 5～10min;
Step 3: step 1 and the pretreated material of step 2 are adopted to alcohol-pickled 10～30s of 70～80%, sterile water wash 2～4 times;
Step 4: adopt 5～10% clorox to soak 5～20min, sterile water wash 4～7 times.
4. the method for explant sterilization in Plant Tissue Breeding according to claim 3, is characterized in that: in described step 1, adopt 0.3% liquid detergent solution soak-out material 10min.
5. the method for explant sterilization in Plant Tissue Breeding according to claim 3, is characterized in that: in described step 1, adopt running water to rinse 30min.
6. the method for explant sterilization in Plant Tissue Breeding according to claim 3, is characterized in that: in described step 2, adopt the running water that contains 3% penicillin to soak 6min, running water rinses 7min.
7. the method for explant sterilization in Plant Tissue Breeding according to claim 3, is characterized in that: step 3: adopt 75% alcohol-pickled material 10s, sterile water wash 3 times.
8. the method for explant sterilization in Plant Tissue Breeding according to claim 3, is characterized in that: adopt 7% clorox to soak 10min, sterile water wash 6 times.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410262451.2A CN104106466A (en) | 2014-06-12 | 2014-06-12 | Explant disinfection method in plant tissue culture |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104885942A (en) * | 2015-05-26 | 2015-09-09 | 福建农林大学 | Chemical disinfection tissue culture method for rhododendron simsii planch |
| CN109588315A (en) * | 2018-12-31 | 2019-04-09 | 西南民族大学 | A kind of sterilization method of bryophyte explant |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102172219A (en) * | 2011-01-29 | 2011-09-07 | 国家林业局泡桐研究开发中心 | Method for carrying out test tube grafting and rejuvenization on paulownia fortunei select tree |
| CN102326514A (en) * | 2011-11-01 | 2012-01-25 | 四川中测科技投资有限公司 | Tea tree explant mercury-free disinfectant and explant disinfection method |
| CN103299902A (en) * | 2013-05-14 | 2013-09-18 | 王台虎 | Method for carrying out tissue culture on chinaberry seedlings |
| CN103704136A (en) * | 2013-12-24 | 2014-04-09 | 中国农业科学院蔬菜花卉研究所 | Tissue culture method for rapidly propagating wild lilies |
-
2014
- 2014-06-12 CN CN201410262451.2A patent/CN104106466A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102172219A (en) * | 2011-01-29 | 2011-09-07 | 国家林业局泡桐研究开发中心 | Method for carrying out test tube grafting and rejuvenization on paulownia fortunei select tree |
| CN102326514A (en) * | 2011-11-01 | 2012-01-25 | 四川中测科技投资有限公司 | Tea tree explant mercury-free disinfectant and explant disinfection method |
| CN103299902A (en) * | 2013-05-14 | 2013-09-18 | 王台虎 | Method for carrying out tissue culture on chinaberry seedlings |
| CN103704136A (en) * | 2013-12-24 | 2014-04-09 | 中国农业科学院蔬菜花卉研究所 | Tissue culture method for rapidly propagating wild lilies |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104885942A (en) * | 2015-05-26 | 2015-09-09 | 福建农林大学 | Chemical disinfection tissue culture method for rhododendron simsii planch |
| CN104885942B (en) * | 2015-05-26 | 2017-01-11 | 福建农林大学 | Chemical disinfection tissue culture method for rhododendron simsii planch |
| CN109588315A (en) * | 2018-12-31 | 2019-04-09 | 西南民族大学 | A kind of sterilization method of bryophyte explant |
| CN109588315B (en) * | 2018-12-31 | 2021-05-28 | 西南民族大学 | Method for disinfecting bryophyte explants |
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Application publication date: 20141022 |