CN1072565A - A kind of Nonpoisonous disinfectant - Google Patents
A kind of Nonpoisonous disinfectant Download PDFInfo
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- CN1072565A CN1072565A CN 92114751 CN92114751A CN1072565A CN 1072565 A CN1072565 A CN 1072565A CN 92114751 CN92114751 CN 92114751 CN 92114751 A CN92114751 A CN 92114751A CN 1072565 A CN1072565 A CN 1072565A
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- wurenye
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Abstract
A kind of avirulent novel disinfectant is to be main active ingredient with Wurenye.Wurenye is that extract and separate is come out from various Chinese herbs a kind ofly has solution more antibacterial, antibiotic, antiviral activity, because of including several chemical compositions that bactericidal effect is arranged such as oleanolic acid, ursolic acid, fumaric acid, succinic acid, benzoic acid in its principal component, so be a kind of avirulence, disinfectant that excitant is low, microorganism concentration is all had a bactericidal effect from 1%~100%.
Description
The present invention relates to a kind of avirulent novel Wurenye disinfectant.
Contain many microorganisms in the air, these microorganisms have very big influence to the mankind's vital movement, breathe microorganism through the people and enter in the human body with air, along with the resistance difference of everyone health, will be to everyone generation physiological change or pathological change in various degree.
According to reports; it is to influence the human life activity via various mediums that 41 kinds of important infectious diseases are arranged in the world; wherein air-borne transmission just has 14 kinds to account for the first place; Britain once carried out infection investigation the beginning of the eighties; the result shows that respiratory tract infection accounts for 30.3%; occupy first; the bacterial infection problem is also paid attention to deeply in the hospital again; in order to prevent that patient's two degree from infecting and protecting the crowd of health in the hospital; must be noted that hospital inner environment microorganism monitoring and sterilization work; countries in the world are already to air sterillization for this reason; purify and to be paid attention to, the various disinfectants of the bright development of broken hair do not have the various microorganisms of harm and then reduce the propagation of various infectious diseases and popular human health and life to reduce in the air.
Develop both at home and abroad at present, can be to the chemosterilant that the body surface air carries out disinfection not following tens kinds of a great variety, all has good Disinfection Effect in general, but these disinfectants all exist toxicity more or less, excitant and corrosivity and contaminated environment etc. are paid effect, unfavorable to health, therefore all can not use in the space that people's activity is arranged, bring very big inconvenience with regard to the industry of carrying out air sterillization for hospital, pharmaceutical factory, food industry urgent need like this, cause its range of application to be subjected to certain restriction.
The objective of the invention is to overcome the drawback of existing disinfectant existence, provide that a kind of to extract a kind of new disinfectant from natural plants be the Wurenye disinfectant, it is not only nontoxic, and have high efficiency and kill various harmful microbe characteristics, can use in the space that people's activity is arranged, be up to the present, the most novel scientific and technological invention in disinfectant.
Wurenye of the present invention is a kind of solution that separating and extracting goes out from various herbal medicine, this solution all has obvious suppression elimination effect to various bacteriums, fungi, virus, through suitably processing, can be used for sterilization, treatment disease, the mildew-resistant storage, fruit, the Vegetable Preservation that also can be used for grain, because it is natural materials, toxicity is low, antibiotic property has tangible economic benefit by force and using value widely.
The chemical analysis of 5 benevolence thimerosals, being colour analysis and the analysis of GC/MS instrument through the extract and separate gas phase, to learn that its main chemical compositions has following several:
(1) fragrant alkanes has certain antibacterial activity as toluene, ethylbenzene, cogongrass etc.
(2) polynary alkanal and ester thereof: as sad, methyl palmitate etc. strong antifungal activity is arranged, anti-cancer activity is still arranged.
(3) phenols: can make albumen precipitation, and change permeability of cell membrane, antibiotic anti-corrosive properties are strong.
(4) benzoic acids: stronger antisepsis and sterilization effect is arranged.
(5) acids: bactericidal effect (seeing accompanying drawing 1-5) is all arranged as oleanolic acid, ursolic acid, fumaric acid, succinic acid, benzoic acid etc.
(6) other: can suppress the second vinegar as furans, and disturb the metabolism of bacterium, You such as Zu class can influence bacterial metabolism and sterilization.
The Wurenye that extracts from natural plants has three kinds of A1, B1, B2 liquid, and wherein A1 liquid is white transparent aqueous solution, slightly has slight tart flavour, dissolves in the organic solvents such as ethanol, ethyl acetate, and proportion is 1.027(10 ℃) pH value is in 5~6 scopes.
Main component content is:
A, phenols are equivalent to the amount of phenol, A1 liquid must not<0.5081mg/ml, B1 liquid must not<0.9448mg/ml, B2 liquid must not<2.3626mg/ml.
B, formic acid class are equivalent to benzoic amount, A1 liquid must not<76.37mg/ml, B1 liquid must not<26.48mg/ml, B2 liquid must not<131.61mg/ml.
The toxicity of Wurenye and bactericidal action can be by showing in the following examples that its effect, these surveys show that embodiment just proposes the characteristic of Wurenye and to the killing effect of microorganism, and do not limit use patent application range of the present invention.
Embodiment one Wurenye acute toxic test
1, get 20 of mouselets, male and female half and half, body weight are divided into two groups at random between 17~20g, give A1 liquid for one group, and irritating stomach dosage is 0.5ml/20g; Another group is given B1 liquid, irritates stomach dosage 0.5ml/20g, observes its situation, and observes continuously a week.None dead mouse (room temperature 15 degree) as a result.
2, healthy rabbits is 4, every about 2 kilograms of body weight, 3 rabbit are given A1, B1, B2 liquid respectively, and irritating stomach dosage is 10ml/2kg, and another gives normal saline solution, irritating stomach dosage also is 10ml/2kg, observe its poisoning situation and death state, observe a week continuously, poisoning symptom and death all do not appear in 4 rabbit, above-mentioned experiment is irritated stomach dosage and is equivalent to 5000mg/kg, is per os poisoning dosage (three times of LD50>1500mg/kg).
Embodiment two, and the oral LD50 of Wurenye measures
Get 50 of mouselets, male and female half and half are divided into five groups at random, dilute gastric infusion according to a conventional method, continue after the administration to observe a week, write down dead situation, and dead animal is in time carried out postmortem examination, record pathology situation is pressed the Bliss method and is calculated, and measurement result learns that LD50 is 1737.90mg/kg.The 95% credible scope of LD50 is 1399.6~1689.6mg/kg, the result learns thus, Wurenye toxicity is low, than phenol solution little (LD50 282mg/kg mouse stomach), also littler than benzoic acid, the administration dead mouse all betides irritated behind the stomach after 24 hours, and mouse is no abnormal when dead, be organized in pathology and dissect, there is no and find to have assorted obvious change.
Embodiment three, skin irritation test
Get the pig mouse of 24 body weight, 250~350 grams, male and female half and half, random packet and be divided into four groups of A1 liquid, B1 liquid, B2 liquid, stoste, every group 6, carry out skin husband irritant test by the DECD method, behind the coating 1 hour, 24 hours, 48 hours, 72 hours respectively, observe the situation of administration part skin husband's excitant (oedema, erythema), and with the skin check plot relatively.
Skin irritation strength assessment standard
Intensity | Score value |
Do not have | 0.0~0.4 |
Slightly | 0.5~1.9 |
Moderate | 2.0~5.9 |
By force | 6.0~8.0 |
Skin irritation test result (room temperature, humidity 73%)
The total score value stimulus intensity of group erythema score value oedema score value
A1 liquid group 0.16 0 0.16 does not have
B1 liquid group 0.33 0 0.33 does not have
B2 liquid group 0.50 0.16 0.66 is slight
Stoste group 1.33 0.50 1.83 is slight
Thus embodiment as can be known, the excitant of Wurenye is very little, A1, B1 be nonirritant almost.
Embodiment four, and the Wurenye Disinfection Effect is measured
1, adopt the MBC method to measure the bactericidal effect of Wurenye to staphylococcus aureus, Escherichia coli, shigella dysenteriae, pseudomonas aeruginosa.
Concentration | Bacterium liquid measure (8 * 10/ml) | Meat soup amount (ml) | Sterilization liquid measure (ml) |
1% | 0.5 | 1.40 | 0.02 |
5% | 0.5 | 1.40 | 0.10 |
10% | 0.5 | 1.30 | 0.20 |
15% | 0.5 | 1.20 | 0.30 |
20% | 0.5 | 1.10 | 0.40 |
Measurement result shows
(1) A1, B2 can reach bactericidal action (asepsis growth) in 10 minutes to Escherichia coli, shigella dysenteriae and pseudomonas aeruginosa effect when 15% concentration.
(2) to Escherichia coli, shigella dysenteriae and pseudomonas aeruginosa effect can reach bactericidal action (asepsis growth) in 5 minutes to B1 liquid when 15% concentration.
(3) B1 liquid can reach bactericidal action in 5 minutes in 20% concentration to golden yellow staphylococcus effect.
2, it is as follows to the sterilization effect result of Bacterium prodigiosum to adopt liquid tube method to measure Wurenye:
The average viable count sterilizing rate t P of decontaminant concentration (%)
A1 100 13.0 79.03 13.57<0.001
50 14.0 82.26 15.37<0.001
1 18.0 70.97 14.31<0.001
0 62.0 0.00
B1 100 0.7 98.87 19.04<0.001
50 11.3 81.77 19.20<0.001
1 20.0 67.74 8.09<0.05
0 62.0
50 0.3 99.48 98.62<0.001
1 37.0 35.65 6.74<0.05
0 57.5
3, it is as follows to the germicidal efficiency result of hay bacillus to adopt liquid tube method to measure Wurenye:
Average viable count sterilizing rate (%) the t P of decontaminant concentration (%)
A1 100 39.3 39.26 4.99<0.01
50 47.7 26.29 3.67<0.05
1 51.0 21.17 2.24<0.05
0 64.7 0.00
B1 100 16.3 67.4 9.01<0.001
50 31.0 38.0 3.71<0.05
1 44.0 11.4 1.52<0.05
0 50.0 0.0
50 8.70 85.74 28.60<0.001
1 64.00 0.00 0.78<0.0
4, adopting liquid tube method to measure Wurenye shows Bacillus tuberculosis's germicidal efficiency result: through repeating 2 times experimental result, at Bacillus tuberculosis's liquid is 10 to 10 o'clock, A1, the B1, the B2 liquid that add 100% concentration respectively acted on after 5 minutes, there is no this bacteria growing.
5, A1 liquid is to gonococcal germicidal efficiency in the employing liquid tube method qualitative determination Wurenye, and the result shows 100%A1 liquid effect 5 minutes, and 50% A1 liquid effect can reach good sterilization effect (asepsis growth) in 10 minutes.
Embodiment five, and Wurenye is to the bactericidal effect of mould:
Adopt liquid tube method to measure the germicidal efficiency of Wurenye to Candida albicans and Aspergillus flavus, the result is as follows:
Disinfectant | Concentration | Average viable count is read the coccus Aspergillus flavus in vain | Sterilizing rate (%) is read the coccus Aspergillus flavus in |
A1 | |||
100 | 0.0 0.0 | 100.0 100.0 | |
50 | 7.0 0.0 | 91.7 100.0 | |
25 | 7.7 7.0 | 90.2 93.0 | |
10 | 54.7 92.0 | 31.0 8.0 | |
1 | 70.4 94.0 | 6.7 6.0 | |
0 | 79.3 100.0 | 0.0 0.0 | |
|
100 | 0.0 0.0 | 100.0 100.0 |
50 | 0.0 0.0 | 100.0 100.0 | |
25 | 0.6 7.0 | 99.3 93.0 | |
10 | 1.0 96.0 | 98.7 4.0 | |
1 | 48.0 102.0 | 39.5 0.0 | |
0 | 79.3 100.0 | 0.0 0.0 | |
|
100 | 0.0 0.0 | 100.0 100.0 |
50 | 0.0 0.0 | 100.0 100.0 | |
25 | 0.0 0.0 | 100.0 100.0 | |
10 | 0.3 0.0 | 99.6 100.0 | |
1 | 4.7 45.0 | 94.1 100.0 | |
0 | 79.3 100.0 | 0.0 0.0 |
Adopt MBC to measure in the Wurenye 30%A1 liquid in addition mould, withered mould, the nigrospora of aspergillus flavus, aspergillus niger, withered mould, wood is had better bactericidal effect.
Embodiment six, and Wurenye is measured the killing effect of virus:
1, the anti-toxic sterilizing effectiveness results of the T-3 Escherichia coli being explained body with liquid tube mensuration Wurenye is as follows: in 10 minutes
The average viable count of decontaminant concentration (class) sterilizing rate (%)
A1 100.0 0.0 100.0
50.0 0.0 100.0
10.0 25.0 96.7
1.0 79.0 86.1
0.0 760.0
B1 100.0 0.0 100.0
50.0 0.0 100.0
10.0 71.0 90.0
1.0 88.0 88.4
0.0 760.0
B2 100.0 0.0 100.0
50.0 0.3 99.9
10.0 54.0 92.9
1.0 72.0 90.5
0.0 760.0
2, adopt the SPRIA method to measure the bactericidal effect of Wurenye to HBsAg:
Get 0.1mlHBsAg positive serum (RPHA method 1: 2048) and add in the 0.4ml variable concentrations Wurenye (A1, B1, B2), act on and add neutralizer effect termination in 30 minutes sterilizing test after 2 minutes, 5 minutes, 10 minutes immediately.Establish the positive (do not add Wurenye and add physiological saline) and negative (do not add the HBsAg positive serum and add physiological saline) simultaneously.Serves as effectively with P/N value<2.1 for declaring feminine gender, and 72.1 for declaring the positive invalid standard that is.
A1 liquid is to HBsAg exercising result (P/N value)
Concentration (%) time
2 minutes 5 minutes 10 minutes
100 0.9 1.1 0.7
50 9.9 9.9 11.6
25 9.9 9.9 15.3
B1 liquid is to HBsAg exercising result (P/N value)
Concentration (%) time
2 minutes 5 minutes 10 minutes
100 1.9 1.6 2.0
50 2.6 2.6 1.8
25 16.3 9.9 9.3
B2 liquid is to HBsAg exercising result (P/N value)
Concentration (%) time
2 minutes 5 minutes 10 minutes
100 1.3 1.1 0.9
50 0.9 0.9 0.7
25 9.9 9.3 3.9
50~100 concentration, acting on 5~10 minutes has Disinfection Effect to HBsAg.
3, Wurenye reaches the lymphocytic damaging action of the mankind the bactericidal effect of being grown disease I type (HIV-1).
Prove that through experimental study A1 liquid is slight to lymphocytic toxicity in the Wurenye, to the HIV-I and killing action is arranged, valid density is between 1: 32~1: 50, and the time is 2~5 minutes.
Description of drawings:
Gas chromatogram after Fig. 1 oleanolic acid methylates
Gas chromatogram after Fig. 2 ursolic acid methylates
Gas chromatogram after Fig. 3 succinic acid methylates
Gas chromatogram after Fig. 4 fumaric acid methylates
Fig. 5 phenol mass spectrogram
Claims (4)
1, a kind of avirulent novel disinfectant is characterized in that containing effective composition Wurenye, microorganisms such as fungi, bacterium and virus are all had the effect of killing, and its concentration produces effect all at 1%~100%.
2, disinfectant as claimed in claim 1 is characterized in that the acids composition in the main component of Wurenye is that oleanolic acid, ursolic acid, fumaric acid, succinic acid, phenol etc. have disinfecting components.
3, disinfectant as claimed in claim 1 is characterized in that can be used for the molten method of liquid processes and gas and carries out disinfection.
4, disinfectant as claimed in claim 1 is characterized in that can be used for the hospital food industry, in crowd activity's environment such as pharmaceutical factory.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN 92114751 CN1072565A (en) | 1992-12-24 | 1992-12-24 | A kind of Nonpoisonous disinfectant |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN 92114751 CN1072565A (en) | 1992-12-24 | 1992-12-24 | A kind of Nonpoisonous disinfectant |
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Publication Number | Publication Date |
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CN1072565A true CN1072565A (en) | 1993-06-02 |
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CN 92114751 Pending CN1072565A (en) | 1992-12-24 | 1992-12-24 | A kind of Nonpoisonous disinfectant |
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CN (1) | CN1072565A (en) |
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1992
- 1992-12-24 CN CN 92114751 patent/CN1072565A/en active Pending
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