JP5455540B2 - How to disinfect a poultry house or barn - Google Patents
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- JP5455540B2 JP5455540B2 JP2009237088A JP2009237088A JP5455540B2 JP 5455540 B2 JP5455540 B2 JP 5455540B2 JP 2009237088 A JP2009237088 A JP 2009237088A JP 2009237088 A JP2009237088 A JP 2009237088A JP 5455540 B2 JP5455540 B2 JP 5455540B2
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- 244000144977 poultry Species 0.000 title claims description 9
- RFFLAFLAYFXFSW-UHFFFAOYSA-N 1,2-dichlorobenzene Chemical compound ClC1=CC=CC=C1Cl RFFLAFLAYFXFSW-UHFFFAOYSA-N 0.000 claims description 48
- 238000002360 preparation method Methods 0.000 claims description 26
- 239000000645 desinfectant Substances 0.000 claims description 21
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 claims description 16
- 238000000034 method Methods 0.000 claims description 15
- 230000000249 desinfective effect Effects 0.000 claims description 6
- 210000003250 oocyst Anatomy 0.000 description 44
- 241000287828 Gallus gallus Species 0.000 description 21
- 235000013330 chicken meat Nutrition 0.000 description 21
- 208000003495 Coccidiosis Diseases 0.000 description 17
- 206010023076 Isosporiasis Diseases 0.000 description 17
- 238000004659 sterilization and disinfection Methods 0.000 description 15
- 241000894006 Bacteria Species 0.000 description 14
- 241000700605 Viruses Species 0.000 description 11
- 210000003608 fece Anatomy 0.000 description 9
- 241000588724 Escherichia coli Species 0.000 description 8
- 230000000694 effects Effects 0.000 description 8
- 230000002147 killing effect Effects 0.000 description 8
- 235000013594 poultry meat Nutrition 0.000 description 6
- 230000000844 anti-bacterial effect Effects 0.000 description 5
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 description 4
- 239000013641 positive control Substances 0.000 description 4
- 206010070834 Sensitisation Diseases 0.000 description 3
- 238000009395 breeding Methods 0.000 description 3
- 230000001488 breeding effect Effects 0.000 description 3
- 244000144972 livestock Species 0.000 description 3
- 239000013642 negative control Substances 0.000 description 3
- 230000003071 parasitic effect Effects 0.000 description 3
- 230000008313 sensitization Effects 0.000 description 3
- 241000193403 Clostridium Species 0.000 description 2
- 241000223932 Eimeria tenella Species 0.000 description 2
- 241000702626 Infectious bursal disease virus Species 0.000 description 2
- 230000001154 acute effect Effects 0.000 description 2
- 239000003242 anti bacterial agent Substances 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 210000004534 cecum Anatomy 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- GRWVQDDAKZFPFI-UHFFFAOYSA-H chromium(III) sulfate Chemical compound [Cr+3].[Cr+3].[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O GRWVQDDAKZFPFI-UHFFFAOYSA-H 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 230000029142 excretion Effects 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 210000004347 intestinal mucosa Anatomy 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 230000000877 morphologic effect Effects 0.000 description 2
- 238000003305 oral gavage Methods 0.000 description 2
- 239000002504 physiological saline solution Substances 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 229960005486 vaccine Drugs 0.000 description 2
- 230000003253 viricidal effect Effects 0.000 description 2
- 208000037384 Clostridium Infections Diseases 0.000 description 1
- 241000224483 Coccidia Species 0.000 description 1
- 206010012735 Diarrhoea Diseases 0.000 description 1
- 206010013647 Drowning Diseases 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 241000223924 Eimeria Species 0.000 description 1
- 208000004232 Enteritis Diseases 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 206010051606 Necrotising colitis Diseases 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 150000001299 aldehydes Chemical class 0.000 description 1
- 230000002155 anti-virotic effect Effects 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 238000011888 autopsy Methods 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 208000027503 bloody stool Diseases 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 150000001896 cresols Chemical class 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 208000035861 hematochezia Diseases 0.000 description 1
- 230000007366 host health Effects 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 208000004995 necrotizing enterocolitis Diseases 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 210000004798 organs belonging to the digestive system Anatomy 0.000 description 1
- 230000017448 oviposition Effects 0.000 description 1
- 201000006195 perinatal necrotizing enterocolitis Diseases 0.000 description 1
- 238000009374 poultry farming Methods 0.000 description 1
- 210000004215 spore Anatomy 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
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Description
本発明は、一度の消毒作業でコクシジウム症の原因であるコクシジウムオーシストを効果的に死滅させると同時に、大腸菌やクロストリジウム（芽胞形成する菌）などの一般細菌ならびにウイルスも効果的に死滅させることのできる鶏舎又は畜舎の消毒方法に関するものである。 The present invention can effectively kill coccidium oocysts that cause coccidiosis in a single disinfection operation, and can also kill general bacteria such as Escherichia coli and Clostridium (spore-forming fungi) and viruses. It relates to the method of disinfecting poultry houses or barns.
コクシジウム症とは、家禽や家畜の消化器官にコクシジウムという原虫が寄生することで、家禽等が下痢や血便などを起こして衰弱してしまう病気であり、一時的な衰弱で回復する場合が多いが、病状が悪化した場合には大量に斃死してしまう例もある。
コクシジウムは原虫のままで感染するものではなく、オーシストと呼ばれる胞子の状態で糞便と共に排出され、その糞便を家禽等が経口摂取してしまうことで感染する。
コクシジウムには様々な種類があり、例えば、Eimeria属の原虫は、主に鶏に寄生して腸炎を主病症とする鶏コクシジウム症を引き起こす。
Coccidiosis is a disease in which a protozoan called coccidia parasitizes the digestive organs of poultry and livestock, causing poultry to become weak due to diarrhea or bloody stool, which often recovers due to temporary weakness. There are also cases where drowning in large quantities occurs when the medical condition worsens.
Coccidium is not infected as a protozoan, but is excreted together with feces in the form of spores called oocysts, and is infected when poultry or the like ingests the feces.
There are various types of coccidiosis, for example, protozoa of the genus Eimeria cause chicken coccidiosis, which is mainly parasitic on chickens and enteritis is the main disease.
鶏コクシジウム症は、腸粘膜内でコクシジウム原虫が多量に増殖することにより腸粘膜が破壊され、栄養や水分の吸収阻害により宿主の健康状態を悪化させる。鶏コクシジウム症の発生率は、鶏が直接糞便に接触し易い平飼い飼育での場合が高いが、ケージ飼育でも多く発生している。また、クロストリジウム感染による壊死性腸炎が併発することが多い。
鶏コクシジウム症は、育成率、飼料要求率および産卵率などの生産成績を著しく悪化させるため、養鶏産業界では非常に問題視されている。
In chicken coccidiosis, the intestinal mucosa is destroyed when a large amount of coccidiosis grows in the intestinal mucosa, and the health of the host is deteriorated by inhibiting absorption of nutrients and water. The incidence of chicken coccidiosis is high in flat breeding where chickens are easily in direct contact with feces, but it is also common in cage breeding. Moreover, necrotizing enterocolitis due to Clostridium infection is often accompanied.
Chicken coccidiosis is a serious problem in the poultry farming industry because it significantly deteriorates the production performance such as breeding rate, feed demand rate and egg-laying rate.
コクシジウム症への対策としては、飼料に抗生物質を添加する方法やワクチンを投与する方法がある。しかしながら、飼料に抗生物質を添加する方法は副作用や薬剤耐性が懸念されているし、ワクチンを投与する方法は全てのコクシジウムに一律に有効ではない。 As countermeasures against coccidiosis, there are a method of adding an antibiotic to a feed and a method of administering a vaccine. However, there are concerns about side effects and drug resistance in the method of adding antibiotics to the feed, and the method of administering the vaccine is not uniformly effective for all coccidium.
また、鶏舎や畜舎に消毒剤を散布する方法もあるが、糞便と共に排泄されたオーシストの状態では一般の消毒剤に対して抵抗性が強く、そのためオルトジクロロベンゼン製剤が広く使用されている。使用されるオルトジクロロベンゼン製剤は、オルトジクロロベンゼンを約７０％、クレゾール類を約１０％含有するものが多い。しかし、その効果は限定的であり十分ではない。 In addition, there is a method of spraying a disinfectant in a poultry house or a livestock house, but in the state of oocysts excreted together with feces, the disinfectant is highly resistant to general disinfectants, and therefore orthodichlorobenzene preparations are widely used. Many of the orthodichlorobenzene formulations used contain about 70% orthodichlorobenzene and about 10% cresols. However, the effect is limited and not sufficient.
また、オルトジクロロベンゼン製剤は、主にコクシジウム症対策が目的であるため、大腸菌などの一般細菌およびウイルスを死滅させるためには他の消毒剤を使用して別途に消毒作業を行う必要がある。 In addition, since orthodichlorobenzene preparations are mainly intended for coccidiosis, it is necessary to separately perform disinfection using other disinfectants in order to kill general bacteria such as E. coli and viruses.
関連する先行技術文献を掲げると、特開平６−１７１９号公報（特許文献１）が存する。この先行技術文献では、コクシジウム症対策としてオルトジクロロベンゼンに塩化ジアルキルジメチルアンモニウムを含有した製剤が提案されている。この発明は、従来のオルソ剤よりも殺オーシスト効果を向上し、更に一般細菌をも死滅できる殺菌消毒剤を提供しようとするものであり、必須成分としてオルトジクロロベンゼンに加えて塩化ジアルキルジメチルアンモニウムを含有させたことを特徴とするものである。 JP-A-6-1719 (Patent Document 1) exists as a related prior art document. This prior art document proposes a preparation containing dialkyldimethylammonium chloride in orthodichlorobenzene as a measure against coccidiosis. The present invention aims to provide a bactericidal disinfectant that improves the oocyst killing effect over conventional ortho-agents and can also kill general bacteria. In addition to orthodichlorobenzene, dialkyldimethylammonium chloride is added as an essential component. It is characterized by containing.
しかしながら、塩化ジアルキルジメチルアンモニウムは大腸菌などの一般細菌に対しては殺菌力が弱い消毒剤であり、コクシジウム症と併発することが多いクロストリジウム・パープリンゲンス(芽胞形成菌)にはほとんど殺菌力がなく、エンベロープがない伝染性ファブリキウス嚢病ウイルス（IBDV：Birnaviridae Avibirnavirus）には無効である。
従って、塩化ジアルキルジメチルアンモニウムによりオルソ剤の殺オーシスト効果を向上したとしても、一般細菌およびウイルスに対する殺滅効果はそれほど高くはない。
However, dialkyldimethylammonium chloride is a disinfectant with a weak bactericidal power against general bacteria such as E. coli, and Clostridium perpuringens (spore-forming bacteria) that often accompanies coccidiosis has little bactericidal power, It is ineffective against infectious bursal disease virus (IBDV) without envelope.
Therefore, even if the orthocidal effect of ortho drugs is improved by dialkyldimethylammonium chloride, the killing effect on general bacteria and viruses is not so high.
上述したように、従来の鶏舎又は畜舎の消毒方法にあっては、コクシジウム症対策としてオルトジクロロベンゼン製剤による消毒作業と、一般細菌およびウイルス対策として他の消毒剤による消毒作業との二度の消毒作業が必要であったため、消毒作業が繁雑である上に時間も掛かっていた。 As described above, in the conventional method for disinfecting poultry houses or livestock houses, disinfection with an orthodichlorobenzene preparation as a countermeasure against coccidiosis and disinfection with another disinfectant as a countermeasure against general bacteria and viruses are performed twice. Because work was necessary, disinfection work was complicated and time consuming.
このため、一度の消毒作業で効果的にコクシジウムオーシストを死滅させ、且つ、一般細菌およびウイルスも同時に死滅させることのできる新たな消毒方法の開発が切望されていたのである。 Therefore, development of a new disinfection method capable of effectively killing coccidiosis oocysts by one disinfection operation and simultaneously killing general bacteria and viruses has been desired.
本発明は、上記実情に鑑み、オルトジクロロベンゼンの殺オーシスト効果を向上すると共に、大腸菌などの一般細菌およびウイルスに対しても十分な殺滅効果があり、一度の消毒作業でコクシジウム対策と一般細菌およびウイルス対策が同時に行える消毒方法の提供を目的として開発されたものであって、請求項１記載の消毒方法では、消毒剤としてオルトジクロロベンゼン製剤とグルタルアルデヒド製剤の混合製剤を使用する構成を採用した。 In view of the above circumstances, the present invention improves the killing effect of orthodichlorobenzene and has a sufficient killing effect against general bacteria such as E. coli and viruses. Was developed for the purpose of providing a disinfecting method capable of simultaneously performing anti-virus measures, and the disinfecting method according to claim 1 employs a configuration in which a mixed preparation of an orthodichlorobenzene preparation and a glutaraldehyde preparation is used as the disinfectant. did.
また、請求項２記載の消毒方法では、請求項１を前提として、混合製剤のオルトジクロロベンゼンの濃度を０．７５重量％から１．５重量％の範囲とし、グルタルアルデヒドの濃度を０．１２５重量％から０．５重量％の範囲とする構成を採用した。 Further, in the disinfection method according to claim 2, on the premise of claim 1, the concentration of orthodichlorobenzene in the mixed preparation is in the range of 0.75 wt% to 1.5 wt%, and the concentration of glutaraldehyde is 0.125. A configuration in which the range of wt% to 0.5 wt% was adopted.
これにより、請求項１及び請求項２記載の消毒方法にあっては、消毒剤として、オルトジクロロベンゼン製剤とグルタルアルデヒド製剤の混合製剤を使用する構成を採用したことにより、オルトジクロロベンゼン製剤の殺オーシスト効果を向上させると同時に、グルタルアルデヒド製剤の強力な殺菌効果および殺ウイルス効果により大腸菌などの一般細菌およびウイルスも同時に死滅させることが可能となる。 Thus, in the disinfection method according to claim 1 and claim 2, by adopting a configuration in which a mixed preparation of an orthodichlorobenzene preparation and a glutaraldehyde preparation is used as the disinfectant, the killing of the orthodichlorobenzene preparation is performed. While improving the oocyst effect, general bacteria and viruses such as Escherichia coli can be simultaneously killed by the powerful bactericidal and virucidal effects of the glutaraldehyde preparation.
従って、一度の消毒作業によりコクシジウムオーシストと大腸菌などの一般細菌およびウイルスの双方を同時に死滅させることができるようになり、消毒作業の簡素化と時間短縮が可能となるのである。 Accordingly, it is possible to simultaneously kill both coccidium oocysts and general bacteria such as Escherichia coli and viruses by a single disinfection operation, and the disinfection operation can be simplified and the time can be shortened.
以下、実施例として本発明の効果を検証した試験とその結果を説明する。 Hereinafter, the test which verified the effect of this invention and its result are demonstrated as an Example.
〔混合製剤の効果試験（コクシジウムオーシストに対する２４時間感作試験）〕
（試験用オーシストの調整）
急性盲腸コクシジウム症に感染している鶏から排泄された糞を採取し、これよりコクシジウムオーシストを回収して胞子形成させた。これを１０日齢ヒナに投与し、排泄オーシストの形態学的特徴および寄生部位等からコクシジウム種の同定を行った。同定の結果、この試験用オーシストはEimeria tenellaと認められた。
この試験用オーシストを５×１０４個／ｇの菌液として調整した。
[Efficacy test of mixed preparation (24-hour sensitization test against coccidium oocysts)]
(Adjustment of test oocysts)
Feces excreted from chickens infected with acute cecal coccidiosis were collected, from which coccidium oocysts were collected and sporulated. This was administered to 10-day-old chicks, and the species of coccidium was identified from the morphological characteristics and parasitic sites of excretion oocysts. As a result of identification, this test oocyst was recognized as Eimeria tenella.
This test oocyst was prepared as a bacterial solution of 5 × 10 4 cells / g.
次に、下記表１のように第１区〜第５区の５種類の試験区を設定した。
第１区は、陽性対照の試験区であり、試験結果を判断する指標である。
第２区は、グルタルアルデヒドを単独で０．１２５重量％含有する消毒剤（検体）を使用した試験区である。
第３区は、オルトジクロロベンゼンとグルタルアルデヒドを夫々０．７５重量％と０．１２５重量％含有する消毒剤（検体）を使用した試験区である。
第４区は、オルトジクロロベンゼンを単独で０．７５重量％含有する消毒剤（検体）を使用した試験区である。
第５区は、陰性対照の試験区である。
そして、前記試験用オーシストを第２区〜第４区の夫々の消毒剤（検体）に２４時間感作させて試験用オーシストの調整を完了した。
Next, as shown in Table 1 below, five types of test zones, the first zone to the fifth zone, were set.
The first section is a positive control test section and is an index for judging the test result.
The second section is a test section using a disinfectant (specimen) containing 0.125% by weight of glutaraldehyde alone.
The third section is a test section using a disinfectant (specimen) containing 0.75 wt% and 0.125 wt% of orthodichlorobenzene and glutaraldehyde, respectively.
The fourth section is a test section using a disinfectant (specimen) containing 0.75% by weight of orthodichlorobenzene alone.
The fifth section is a negative control test section.
Then, the test oocysts were sensitized to the respective disinfectants (specimens) in the second to fourth zones for 24 hours to complete the adjustment of the test oocysts.
（供試鶏の調整）
供試鶏は、白色レグホーン系雄初生ヒナを孵化場より導入し、導入後１３日齢まで電熱バタリー式ブルーダーでコクシジウムの感染を予防して飼育した。なお、実際の試験は翌日に行ったので供試鶏は１４日齢ヒナとなる。
(Adjustment of test chicken)
The test chickens were bred by introducing white leghorn male chicks from the hatchery and preventing infection with coccidium with an electric heat butterfly broder until 13 days after introduction. Since the actual test was conducted on the next day, the test chickens were 14-day-old chicks.
（試験の実施）
前記第２区〜第４区では、夫々の濃度の消毒剤に２４時間感作させた試験用オーシストを生理食塩水に浮遊させ、遠心処理をした後、１羽あたり５×１０４個となるように供試鶏のそ嚢内に単回強制経口投与した。
(Examination of test)
In the second to fourth zones, test oocysts sensitized to various concentrations of the disinfectant for 24 hours are suspended in physiological saline, centrifuged, and 5 × 10 4 per bird. As described above, a single oral gavage was administered into the sac of the test chicken.
各供試鶏は試験用オーシストの経口投与後７日目で剖検し、盲腸よりコクシジウムオーシストの回収を行い、クロム硫酸法により糞便１ｇあたりのオーシスト数（ＯＰＧ）を測定した。
その結果を下記表２に示す。
Each test chicken was necropsied 7 days after the oral administration of test oocysts, coccidium oocysts were collected from the cecum, and the number of oocysts per gram of feces (OPG) was measured by the chromium sulfate method.
The results are shown in Table 2 below.
（試験の結果）
まず、第１区（陽性対照）では、全ての供試鶏について糞便１ｇあたりのオーシスト数（ＯＰＧ）が一様に上昇し、オーシストの増殖が正常に進行したことが認められた。
また、第５区（陰性対照）では、いずれの供試鶏からもオーシストは発見されなかったので、いずれの供試鶏も試験終了まで試験用オーシスト以外のコクシジウムオーシストには接触していないことが確認された。
(Test results)
First, in the first section (positive control), the number of oocysts per gram of feces (OPG) was uniformly increased for all test chickens, and it was observed that the growth of oocysts proceeded normally.
In the 5th section (negative control), no oocysts were found in any test chicken, so that none of the test chickens were in contact with coccidium oocysts other than the test oocyst until the end of the test. confirmed.
これを前提として、第３区（オルトジクロロベンゼン＋グルタルアルデヒド混合）の結果を見ると、第３区は、第２区（グルタルアルデヒド単独）および第４区（オルトジクロロベンゼン単独）に比べて有意なオーシスト数（ＯＰＧ）の低下を認めた。
従って、オルトジクロロベンゼン製剤単独やグルタルアルデヒド製剤単独の消毒剤よりも両製剤を混合した消毒剤の方が相乗的に殺オーシスト効果を向上することが実証された。
Based on this assumption, looking at the results of the third section (orthodichlorobenzene + glutaraldehyde mixture), the third section is more significant than the second section (glutaraldehyde alone) and the fourth section (orthodichlorobenzene alone). A decrease in the number of oocysts (OPG) was observed.
Therefore, it was demonstrated that the disinfectant mixed with both preparations synergistically improves the oocyst effect compared to the disinfectant of the orthodichlorobenzene preparation alone or the glutaraldehyde preparation alone.
〔混合製剤の濃度変化よる効果試験（コクシジウムオーシストに対する３時間感作試験）〕
（試験用オーシストの調整）
急性盲腸コクシジウム症に感染している鶏から排泄された糞を採取し、これよりコクシジウムオーシストを回収して胞子形成させた。これを１０日齢ヒナに投与し、排泄オーシストの形態学的特徴および寄生部位等からコクシジウム種の同定を行った。同定の結果、この試験用オーシストはEimeria tenellaと認められた。
この試験用オーシストを５×１０４個／ｇの菌液として調整した。この試験用オーシストの調整は前記実施例１と同様である。
[Effectiveness test by changing the concentration of the mixed preparation (3-hour sensitization test against coccidiosis)
(Adjustment of test oocysts)
Feces excreted from chickens infected with acute cecal coccidiosis were collected, from which coccidium oocysts were collected and sporulated. This was administered to 10-day-old chicks, and the species of coccidium was identified from the morphological characteristics and parasitic sites of excretion oocysts. As a result of identification, this test oocyst was recognized as Eimeria tenella.
This test oocyst was prepared as a bacterial solution of 5 × 10 4 cells / g. The adjustment of the test oocyst is the same as that in Example 1.
次に、下記表３のように第１区〜第５区の５種類の試験区を設定した。
第１区は、陽性対照の試験区であり、試験結果を判断する指標である。
第２区〜第４区は、オルトジクロロベンゼンを０．７５重量％〜１．５重量％の範囲で、グルタルアルデヒドを０．１２５重量％〜０．５重量％の範囲で濃度を変化させた消毒剤（検体）を使用した試験区である。
第５区は、陰性対照の試験区である。
そして、前記試験用オーシストを第２区〜第４区の夫々の消毒剤（検体）に３時間感作させて試験用オーシストの調整を完了した。
Next, as shown in Table 3 below, five types of test zones from the first zone to the fifth zone were set.
The first section is a positive control test section and is an index for judging the test result.
In the second to fourth zones, the concentration of orthodichlorobenzene was changed in the range of 0.75 wt% to 1.5 wt%, and the concentration of glutaraldehyde was changed in the range of 0.125 wt% to 0.5 wt%. This is a test section using a disinfectant (specimen).
The fifth section is a negative control test section.
Then, the test oocysts were sensitized to the respective disinfectants (specimens) in the second to fourth zones for 3 hours to complete the adjustment of the test oocysts.
（供試鶏の調整）
供試鶏は、白色レグホーン系雄初生ヒナを孵化場より導入し、導入後１３日齢まで電熱バタリー式ブルーダーでコクシジウムの感染を予防して飼育した。なお、実際の試験は翌日に行ったので供試鶏は１４日齢ヒナとなる。この供試鶏の調整は実施例１の試験と同様である。
(Adjustment of test chicken)
The test chickens were bred by introducing white leghorn male chicks from the hatchery and preventing infection with coccidium with an electric heat butterfly broder until 13 days after introduction. Since the actual test was conducted on the next day, the test chickens were 14-day-old chicks. Adjustment of this test chicken is the same as the test of Example 1.
（試験の実施）
前記の第２区〜第４区では夫々の濃度の消毒剤に３時間感作させた試験用オーシストを生理食塩水に浮遊させ、遠心処理をした後、１羽あたり５×１０４個となるように供試鶏のそ嚢内に単回強制経口投与した。
(Examination of test)
In the second to fourth zones, the test oocysts sensitized to each concentration of the disinfectant for 3 hours are suspended in physiological saline, centrifuged, and 5 × 10 4 per bird. As described above, a single oral gavage was administered into the sac of the test chicken.
オーシスト経口投与後７日目で剖検し、盲腸よりコクシジウムオーシストの回収を行い、クロム硫酸法により糞便１ｇ当たりのオーシスト数（ＯＰＧ）を測定した。
その結果を下記表４に示す。
On day 7 after oral administration of oocysts, autopsy was performed, and coccidium oocysts were collected from the cecum, and the number of oocysts (OPG) per gram of feces was measured by the chromium sulfate method.
The results are shown in Table 4 below.
（試験の結果）
試験用オーシストをオルトジクロロベンゼンとグルタルアルデヒドの混合製剤に感作させた第２区，第３区，第４区は、第１区（陽性対照）に比べて有意なコクシジウムオーシストの低下を認めた。
(Test results)
In the 2nd, 3rd, and 4th groups, the test oocysts were sensitized to a mixed preparation of orthodichlorobenzene and glutaraldehyde, a significant decrease in coccidium oocysts was observed compared to the 1st group (positive control). .
従って、僅か３時間の感作であっても、オルトジクロロベンゼンとグルタルアルデヒドの混合製剤を使用することは有効な殺オーシスト効果が発揮されることが認められた。 Therefore, it was confirmed that even if the sensitization is only 3 hours, the use of a mixed preparation of orthodichlorobenzene and glutaraldehyde exhibits an effective oocystic effect.
さらに、第２区〜第４区のオーシスト数（ＯＰＧ）の減少に大差がないことからして、オルトジクロロベンゼンとグルタルアルデヒドの夫々の濃度範囲が当該試験の濃度範囲にあれば一様に殺オーシスト効果を発揮することが認められた。 Furthermore, since there is no significant difference in the decrease in the number of oocysts (OPG) in the 2nd to 4th sections, if the respective concentration ranges of orthodichlorobenzene and glutaraldehyde are within the concentration range of the test, they are uniformly killed. It was confirmed that the oocyst effect was exhibited.
以上のように、実施例１および実施例２によれば、オルトジクロロベンゼン製剤とグルタルアルデヒド製剤を混合した消毒剤を使用することにより、従来のオルソ剤よりも殺オーシスト効果を向上できると共に、グルタルアルデヒドの強力な殺菌効果および殺ウイルス効果により、大腸菌などの一般細菌およびウイルスも同時に死滅させることができるようになる。
これにより、一度の消毒作業でコクシジウムオーシストと大腸菌などの一般細菌およびウイルスを同時に死滅させることができるので、従来のようにコクシジウム対策の消毒作業と一般細菌およびウイルス対策の消毒作業とを別々に実施する必要がなくなり、頗る効率的な消毒作業が行えることとなる。
As described above, according to Example 1 and Example 2, by using a disinfectant obtained by mixing an orthodichlorobenzene preparation and a glutaraldehyde preparation, the oocyst killing effect can be improved as compared with the conventional ortho-agent, and glutar Due to the strong bactericidal and virucidal effects of aldehydes, general bacteria such as E. coli and viruses can be killed simultaneously.
As a result, coccidium oocysts and general bacteria such as Escherichia coli and viruses can be killed at the same time by one disinfection operation, so the disinfection work for coccidium measures and the disinfection work for general bacteria and virus measures are performed separately as before. This eliminates the need to perform an effective disinfection operation.
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