CN1072492C - Use of basket fern effective parts in preparing antimalarial drug - Google Patents
Use of basket fern effective parts in preparing antimalarial drug Download PDFInfo
- Publication number
- CN1072492C CN1072492C CN97112454A CN97112454A CN1072492C CN 1072492 C CN1072492 C CN 1072492C CN 97112454 A CN97112454 A CN 97112454A CN 97112454 A CN97112454 A CN 97112454A CN 1072492 C CN1072492 C CN 1072492C
- Authority
- CN
- China
- Prior art keywords
- effective parts
- basket fern
- basket
- fern effective
- parts
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicines Containing Plant Substances (AREA)
- Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)
Abstract
The present invention discloses the new use of the effective parts of cyrtomii rhizoma in preparing medicine, more specifically the application of the effective parts of cyrtomii rhizome in preparing antimalarial medicines.
Description
The present invention relates to a kind of new pharmaceutical use of effective ingredient in Chinese, specifically is the application of basket fern effective parts in the preparation antimalarial drug, belongs to drug world.
Rhizoma Osmundae is a kind of Chinese herbal medicine commonly used, its nature and flavor hardship, cold, and medicinal function is parasite killing, heat-clearing and toxic substances removing, cooling blood for hemostasis.Control anemopyretic cold, warm macule is spitted blood discharging fresh blood stool, dysentery, metrorrhagia, leukorrhagia.For the research of Rhizoma Osmundae, find that at present it mainly acts on: anthelmintic action, antivirus action, antibacterial action and to the effect in uterus.Mainly Rhizoma Osmundae is used for preventing cold, prevention epidemic cerebrospinal meningitis, treatment biliary ascariasis clinically, is used to shrink uterus etc.Up to the present, the relevant basket fern effective parts of Shang Weijian is used for antimalarial report.
The object of the invention provides the new medicine use of Chinese herbal medicine Rhizoma Osmundae, that is, and and the application of basket fern effective parts in the preparation antimalarial drug.
Basket fern effective parts of the present invention is the effective ingredient that extracts from the Chinese herbal medicine Rhizoma Osmundae according to the extraction process of routine, the effective ingredient that preferably extracts from dryopteris crassirhizoma Nakai.
The extracting method of basket fern effective parts is as follows:
Get the dryopteris crassirhizoma Nakai medical material, after the pulverizing, extract with aether backflow, extracting solution is concentrated into half of original volume, room temperature is placed 1-2 week, separates out the yellowish-brown precipitation, filter collection precipitation, stir after fully dissolving with ether, sucking filtration, so repeated treatments to filtrate be faint yellow till, the precipitation natural drying, get buff powder, be basket fern effective parts.Yield is generally 2.2-2.6%, and fusing point is 180-183 ℃, identifies through the thin layer chromatography chromatograph, wherein contains dryocrassin (dryocrassin) more than 80%.
Basket fern effective parts of the present invention also can be prepared into the pure product of dryocrassin.
After the pure product of basket fern effective parts of the present invention and dryocrassin are prepared into medicament, has antimalarial effect.
According to conventional formulation technology, basket fern effective parts of the present invention and the pure product of dryocrassin can be prepared into any pharmaceutical preparation that is applicable to clinical use, for example, capsule powder, pill, unguentum, oral liquid, injection etc.
The method that basket fern effective parts is prepared into tablet is as follows:
Get the dryopteris crassirhizoma Nakai medical material, after the pulverizing, extract with aether backflow, extracting solution is concentrated into half of original volume, room temperature is placed 1-2 week, separates out the yellowish-brown precipitation, filter collection precipitation, stir after fully dissolving with ether, sucking filtration, so repeated treatments to filtrate be faint yellow till, the precipitation natural drying, get buff powder, be basket fern effective parts.Get 1 part of basket fern effective parts, add 10 parts of medical starches, mix homogeneously is made soft material, pelletize, and granulate is gone into the tablet machine tabletting, makes tablet.
Through experimentation, the pure product of basket fern effective parts of the present invention and dryocrassin that prove have good antimalarial effect.
Experimental example 1 basket fern effective parts of the present invention is to NK173 strain Bai Shi Mus plasmodium ED
50And SD
50Mensuration one, experiment material
Worm strain: NK173 strain Bai Shi Mus plasmodium.
Medicine: No. one, malaria (containing basket fern effective parts) is provided by Beijing University of Chinese Medicine.
Animal: Kunming mouse is provided by China Academy of TCM's animal center.
Instrument: OLYMPUS microscope.Two, test method and result
Conventional four days inhibition methods are adopted in experiment, Kunming mouse, body weight 18-21 gram.Male, be divided into 6 groups at random, every group of 10 animals, the abdominal cavity quantitatively inoculates 1 * 10
7Individual Mus plasmodium/only, dosage is respectively 50.4,72,103,147,210,300mg/kg, other establish one group for the inoculation matched group, behind the inoculated malaria protozoon, the same day oral administration, once a day, continuous four days, afterbody was got blood smear in the 5th day, the dyeing of Giemsa staining, microscopic examination plasmodium turn out cloudy situation and infected mice plasmodium number calculate negative conversion rate and infection rate.The results are shown in Table ED of table malaria
50And SD
50Measure
Group | Dosage mg/kg | Number of animals | Parasitic rate % | Suppression ratio % | Negative conversion rate % |
No. one, Inoculant contrast malaria | 50.4 72 103 147 210 300 | 10 10 10 10 10 10 10 | 13.5±4.16 6.27±2.73 1.59±1.49 0.80±0.95 1.01±1.29 0.03±0.06 0 | - 53.6 88.2 94.1 92.5 99.8 100 | 0 0 10 10 30 60 100 |
No. one, malaria is to NK173 strain Bai Shi Mus plasmodium ED
50Be 174.4mg/kg (95% fiducial limit 146.3-207.8mg/kg), SD
50Be 47.6mg/kg (95% fiducial limit 29.4-77.07mg/kg).
After measured, the LD of basket fern effective parts of the present invention
50Be 802.7mg/kg.Being calculated as follows of its therapeutic index:
LD
50/ED
50=802.7/174.4=4.60
LD
50/SD
50=802.7/47.6=16.9
Experiment showed, that basket fern effective parts has fine therapeutical effect to malaria.Mensuration (the SD of No. 2 antimalarial actives of experimental example
50) (ED
50) 1, experiment material
Medicine: No. one, malaria (containing basket fern effective parts), provide by Beijing University of Chinese Medicine chemistry chamber, be made into suspension with distilled water and a little tween 80.
Animal: Kunming mouse, male and female half and half, body weight 18-22g is provided by China Academy of TCM's animal center.
Worm strain: NK173 strain Bai Shi Mus plasmodium and anti-chloroquine strain NK173 Bai Shi Mus plasmodium.2, test method
Conventional four days inhibition methods are adopted in experiment, and Kunming mouse is pressed the body weight random packet, every group of 10 animals, and the abdominal cavity quantitatively inoculates 1 * 10
7The plasmodial erythrocyte liquid of individual infection NK173 strain Bai Shi Mus 0.2ml establishes 6 dosage groups, is respectively 50.4,72,103,147,210,300mg/kg, and other establishes the inoculation matched group; The quantitatively inoculation 1 * 10 of same abdominal cavity
7The plasmodial erythrocyte liquid of the anti-chloroquine strain of individual infection NK173 Bai Shi Mus 0.2ml, if 5 dosage groups are respectively 50.4,72,103,147,210mg/kg, other establishes the inoculation matched group, inoculated malaria protozoon oral administration on the same day, once a day, continuous four days, afterbody was got blood smear in the 5th day, the dyeing of Giemsa staining, the microscopic examination protozoon situation of turning out cloudy is calculated the plasmodium infection rate that every treated animal is counted in its negative conversion rate and microscopic examination according to the zoometer of turning out cloudy in the group, calculates half negative conversion rate ED
50And half suppression ratio SD
50
Antimalarial active to two groups of worm strains of malaria relatively sees the following form.
Conclusion: the essentially no cross tolerance of malaria antagonism chloroquine strain.
Dosage mg/kg | Normal strain | Anti-chloroquine strain | ||
Negative conversion rate % | Suppression ratio % | Negative conversion rate % | Suppression ratio % | |
50.4 72 103.0 147.0 210.0 300.0 | 0.0 0.0 10.0 33.3 60.0 100.0 | 53.6 88.2 94.1 92.5 99.8 100.0 | 0.0 0.0 10.0 10.0 60.0 / | 58.1 76.6 90.4 95.2 99.5 / |
Median effective dose (mg/kg) | 174.4 (ED 50) | 47.6 (SD 50) | 183.8 (ED 50) | 49.5 (SD 50) |
Claims (4)
1, a kind of basket fern effective parts is characterized in that this effective site is made by following method: get the dryopteris crassirhizoma Nakai medical material, after the pulverizing, extract with aether backflow, extracting solution is concentrated into half of original volume, and room temperature is placed 1-2 week, separate out the yellowish-brown precipitation, the filter collection precipitates, and stirs sucking filtration after fully dissolving with ether, so repeated treatments to filtrate be faint yellow till, the precipitation natural drying gets buff powder, is basket fern effective parts.
2, basket fern effective parts as claimed in claim 1, the application in the preparation anti-malaria medicaments.
3, basket fern effective parts as claimed in claim 1 is characterized in that this effective site Main Ingredients and Appearance is a dryocrassin.
4, the application of dryocrassin as claimed in claim 3 in the preparation anti-malaria medicaments.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN97112454A CN1072492C (en) | 1997-06-13 | 1997-06-13 | Use of basket fern effective parts in preparing antimalarial drug |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN97112454A CN1072492C (en) | 1997-06-13 | 1997-06-13 | Use of basket fern effective parts in preparing antimalarial drug |
Publications (2)
Publication Number | Publication Date |
---|---|
CN1202367A CN1202367A (en) | 1998-12-23 |
CN1072492C true CN1072492C (en) | 2001-10-10 |
Family
ID=5172270
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN97112454A Expired - Fee Related CN1072492C (en) | 1997-06-13 | 1997-06-13 | Use of basket fern effective parts in preparing antimalarial drug |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN1072492C (en) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN100340254C (en) * | 2004-08-30 | 2007-10-03 | 孙岭岗 | Medicine composition for treating malaria |
-
1997
- 1997-06-13 CN CN97112454A patent/CN1072492C/en not_active Expired - Fee Related
Non-Patent Citations (1)
Title |
---|
《中药大辞典》 1994.1.1 江苏新医学院 * |
Also Published As
Publication number | Publication date |
---|---|
CN1202367A (en) | 1998-12-23 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN1704085A (en) | Pharmaceutical composition with immunoregulation function and method for preparing the same | |
CN101152324A (en) | Method for producing Yujin composition and medicament containing the same | |
CN104547826B (en) | Galangal rhizome extract treats the medical usage of dysmenorrhoea | |
WO2006118553A1 (en) | Compositions comprising natural agents for the treatment of hiv-associated opportunistic infections and complications and methods for preparing and using compositions comprising natural agents | |
CN1706397B (en) | Composition of paeoniflorin and peony lactone glycoside with function of increasing leukocyte | |
CN1927293A (en) | Chinese medicine composition for treating skin burn and scald | |
CN1072492C (en) | Use of basket fern effective parts in preparing antimalarial drug | |
CN1293101C (en) | White fungus polysaccharide and its preparation method as well as medicament composition with the compound as active constituent | |
CN1304769A (en) | Blood-cleaning pill | |
CN1480171A (en) | Preparation for fish gold and its preparing method | |
CN1060943C (en) | Composite pharmaceutics made of oldenlandia and usage thereof | |
CN1067258C (en) | Medicinal preparation with extractive from yangtao root and preparing process thereof | |
CN101485751B (en) | Cotinus coggygria compound preparation and use | |
CN1839867A (en) | Medicinal composition with heat-clearing, fire-draining and detoxification function | |
CN1045885C (en) | Method for preparing stomatitis spray | |
CN1097139A (en) | The compounded solid oral medicine of treatment upper respiratory tract infection | |
CN1197595C (en) | Chinese medicine preparation for broad-spectrum inflammation relieving, disinfecting, erosion prevention and muscle growing | |
CN1478520A (en) | Heat-clearing and detoxic medicine and its preparation method | |
CN106902330B (en) | Traditional Chinese medicine preparation for treating rheumatic arthritis and rheumatalgia and preparation method and application thereof | |
CN1883521A (en) | Application of Rangoon creeper fruit and its extracts in preparation of medicine for promoting lead expelling | |
CN1247222C (en) | Pharmaceutical composition for treating AlDS | |
CN1177592C (en) | Myocarditis treating medicine | |
CN1559550A (en) | New type medicina preparation with one chinese medicinal herb extract ant its preparation method | |
CN1094620A (en) | The Chinese patent medicine Liu Hua ai huixiangdan of the treatment esophageal carcinoma | |
CN1255108C (en) | Prepn process of Xuesaitong guttate pills |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
C06 | Publication | ||
PB01 | Publication | ||
C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
C19 | Lapse of patent right due to non-payment of the annual fee | ||
CF01 | Termination of patent right due to non-payment of annual fee |