CN107235881A - The supercritical carbon dioxide extraction method of astaxanthin in transgenic engineering tamato fruit - Google Patents
The supercritical carbon dioxide extraction method of astaxanthin in transgenic engineering tamato fruit Download PDFInfo
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- C07C403/00—Derivatives of cyclohexane or of a cyclohexene or of cyclohexadiene, having a side-chain containing an acyclic unsaturated part of at least four carbon atoms, this part being directly attached to the cyclohexane or cyclohexene or cyclohexadiene rings, e.g. vitamin A, beta-carotene, beta-ionone
- C07C403/24—Derivatives of cyclohexane or of a cyclohexene or of cyclohexadiene, having a side-chain containing an acyclic unsaturated part of at least four carbon atoms, this part being directly attached to the cyclohexane or cyclohexene or cyclohexadiene rings, e.g. vitamin A, beta-carotene, beta-ionone having side-chains substituted by six-membered non-aromatic rings, e.g. beta-carotene
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Abstract
The supercritical carbon dioxide extraction method of astaxanthin in transgenic engineering tamato fruit, it is characterized in that this method includes taking the astaxanthin engineering tamato fruit dry powder sieved in advance, regulating system temperature and pressure is to setting value, the temperature and pressure of kettle to be extracted are reached after setting value and stabilization, carry out 1h static extracting；Static extracting opens constant-flux pump after terminating, and entrainer is transported into equipment with 1.0g/min, and the fluid-mixing of entrainer and carbon dioxide enters extraction kettle, starts dynamic extraction.This method materials safety, recovery rate is high, is especially suitable for the extraction of transgenic engineering tomato astaxanthin.In addition, the carotenoid such as lycopene and bata-carotene containing high nutritive value in transgenic engineering tomato, under the optimum extraction condition of astaxanthin, lycopene is 82%, and bata-carotene recovery rate is 89%.Astaxanthin, which is extracted, for the industrialization of transgenic engineering tomato has established technical foundation.
Description
Technical field：
The invention belongs to biological technical field, in particular it relates in a kind of transgenic engineering tamato fruit astaxanthin it is super
Critical carbon dioxide fluid extraction process, while also providing the overcritical dioxy of carotenoid in transgenic engineering tamato fruit
Change carbon fluid extraction process.
Background technology：
Astaxanthin is antioxidation activity most strong bioactive substance in nature, with the hair of plant gene engineering technology
Exhibition, astaxanthin is produced using plant especially industrial crops as bioreactor turns into the focus studied both at home and abroad, astaxanthin
It is the unique keto-acid carotenoid of molecular structure, is antioxidation activity most strong bioactive substance in nature, with anti-spoke
Penetrate, effect of the disease such as anti-aging, antitumor and prevention of cardiovascular, applied to the side such as cosmetics, health products and aquaculture
Face.Commercialized natural astaxanthin is mainly derived from haematococcus pluvialis, and the algae is photoautotrophy green alga, environmentally sensitive, growth
Slowly highdensity cell growth, is difficult to, this is that can not to break through its yield bottleneck and astaxanthin price both at home and abroad at present high
Expensive main cause.Plant is due to a lack of carotenoid assimilation enzyme (BKT) without synthesizing astaxanthin, and the BKT of expression external source may make
Plant especially industrial crops turn into efficient production of astaxanthin factory.Huang Junchao etc. will be sieved by technique for gene engineering in green alga
Select and be separated to the assimilation enzyme and '-hydroxylase gene that can be catalyzed beta carotene and luteole into astaxanthin in plant cell and be transferred to
In wild-type tomatoes plant, astaxanthin in a kind of transgenic engineering tomato rich in astaxanthin, its ripening fruits is obtained first
Content be up to 16.1mg/g, overcome plant be difficult to accumulate high content astaxanthin problem, with such a transgenic engineering tomato
There is great commercial promise as bioreactor production astaxanthin.
Astaxanthin is more sensitive to light, temperature, and in process is extracted, the several factors such as light, heat, oxygen can promote or accelerate
Astaxanthin is degraded；And traditional organic solvent extraction can not be removed because of residue completely, and human body is damaged, broken
Bad body immune system, causes the generation of the diseases such as cancer.Supercritical carbon dioxide extraction is a kind of new extraction skill
Art, the double action with extraction and separation, extraction process material is thus saved substantially, and technological process is simple without phase transformation, extraction
Take efficiency high, organic solvent-free residual, good product quality, non-environmental-pollution.Herein to supercritical carbon dioxide extraction skill
The technique that art extracts astaxanthin from astaxanthin engineering tomato has done preliminary discussion, while to having high exploitation value in extract
Lycopene, the bata-carotene of value are analyzed, to have established technology using transgenic engineering tomato industrialization production astaxanthin
Basis.At present, it there are no and carried using supercritical carbon dioxide extraction technology from transgenic engineering tomato in the prior art
Take the report of the method for astaxanthin.Also the supercritical carbon dioxide fluid without carotenoid in transgenic engineering tamato fruit
The report of extracting process.
The content of the invention：
It is an object of the invention to part in view of the shortcomings of the prior art supercritical carbon dioxide is utilized there is provided one kind
The method that fluid extraction technology extracts astaxanthin from transgenic engineering tomato, while providing from transgenic engineering tamato fruit
The supercritical carbon dioxide extraction method of carotenoid.
In order to realize the above-mentioned purpose of the present invention, the invention provides following technical scheme：
The supercritical carbon dioxide extraction method of astaxanthin in transgenic engineering tamato fruit, this method is pre- including taking
The astaxanthin engineering tamato fruit dry powder first sieved, regulating system temperature and pressure to setting value, the temperature and pressure of kettle to be extracted
Power is reached after setting value and stabilization, carries out 1h static extracting；Static extracting opens constant-flux pump after terminating, and will be pressed from both sides with 1.0g/min
Band agent is transported into equipment, and the fluid-mixing of entrainer and carbon dioxide enters extraction kettle, starts dynamic extraction, so
After carry out saponification.
As mentioned in transgenic engineering tamato fruit astaxanthin supercritical carbon dioxide extraction method, this method
Astaxanthin ester is further completely converted into free state astaxanthin using saponification means, the extract of astaxanthin engineering tomato is entered
Row saponification, takes 1ml extract solutions, is concentrated and done using concentrating instrument, is dissolved in 1ml ether, adds 1ml KOH MeOH and mixes, 0 DEG C of dark
15min is reacted in environment, 2ml 10%NaCl is added and mixes, 2000g centrifugation 2min remove aqueous phase, washed with 2ml 10%NaCl
Twice, it is dissolved in loading after 1ml acetone solns, suction filtration and determines pigment content.
As mentioned in transgenic engineering tamato fruit astaxanthin supercritical carbon dioxide extraction method, take in advance
The tamato fruit dry powder of sieving, regulating system temperature is to 50 DEG C, and extracting pressure 35MPa, the temperature and pressure of kettle to be extracted reach
After setting value and stabilization, 1h static extracting is carried out；Static extracting opens constant-flux pump after terminating, and absolute ethyl alcohol is transported to and set
In standby, the fluid-mixing of absolute ethyl alcohol and carbon dioxide enters extraction kettle, starts dynamic extraction, and dynamic extraction time is
2h。
Carotenoid supercritical carbon dioxide extraction method in transgenic engineering tamato fruit, this method includes
Take the tamato fruit dry powder sieved in advance, regulating system temperature to 50 DEG C, extracting pressure 35MPa, the temperature and pressure of kettle to be extracted
Power is reached after setting value and stabilization, carries out 1h static extracting；Static extracting opens constant-flux pump after terminating, and absolute ethyl alcohol is conveyed
To into equipment, the fluid-mixing of absolute ethyl alcohol and carbon dioxide enters extraction kettle, starts dynamic extraction, dynamic extraction
Time is 2h.
The carotenoid supercritical carbon dioxide extraction method in transgenic engineering tamato fruit, is somebody's turn to do as mentioned
Method is further quantitative determined to carotenoid content, and described carotenoid is astaxanthin, lycopene, β carrots
Element, its method for quantitatively determining is used：Accurate weigh after each 5mg of astaxanthin, lycopene, bata-carotene standard items is mixed uses acetone
Dissolve and be settled to 100ml, take 1ml, 5ml, 10ml mixed dissolution liquid is settled to 50ml with acetone, 1ug/ml is made respectively,
5ug/ml, 10ug/ml standard items aggregate sample, standard curve is made using UPLC loadings, calculates astaxanthin, lycopene, β Hu trailing plants
Bu Su content, described UPLC methods are：Flow velocity：1ml/min；Sample size：5ul；0-1.0min：Water：20%, acetonitrile：
60%, isopropanol：5%, methanol：15%；1.00-2.00min：Water：0%, acetonitrile：80%, isopropanol：5%, methanol：15%；
2.00-8.00min：Water：0%, acetonitrile：80%, isopropanol：5%, methanol：15%；
The supercritical carbon dioxide extraction method of lycopene in transgenic engineering tamato fruit, this method includes taking
The tamato fruit dry powder sieved in advance, regulating system temperature to 50 DEG C, extracting pressure 35MPa, the temperature and pressure of kettle to be extracted
Reach after setting value and stabilization, carry out 1h static extracting；Static extracting opens constant-flux pump after terminating, and absolute ethyl alcohol is transported to
Into equipment, the fluid-mixing of absolute ethyl alcohol and carbon dioxide enters extraction kettle, starts dynamic extraction, during dynamic extraction
Between be 2h.
The supercritical carbon dioxide extraction method of bata-carotene in transgenic engineering tamato fruit, this method includes taking
The tamato fruit dry powder sieved in advance, regulating system temperature to 50 DEG C, extracting pressure
35MPa, the temperature and pressure of kettle to be extracted are reached after setting value and stabilization, carry out 1h static extracting；Static state extraction
Constant-flux pump is opened after taking end, absolute ethyl alcohol is transported into equipment, the mixed flow of absolute ethyl alcohol and carbon dioxide
Body enters extraction kettle, starts dynamic extraction, dynamic extraction time is 2h.
The present invention is to extract astaxanthin from transgenic engineering tomato using supercritical carbon dioxide extraction technology.
In invention, 50 DEG C of extraction temperature, extracting pressure 35MPa, in the case of extracting 2h, the recovery rate of astaxanthin is up to 95%, this side
Method materials safety, recovery rate is high, is especially suitable for the extraction of transgenic engineering tomato astaxanthin.In addition, in transgenic engineering tomato
The carotenoid such as lycopene and bata-carotene containing high nutritive value, under the optimum extraction condition of astaxanthin, tomato red
Plain recovery rate is 82%, and bata-carotene recovery rate is 89%.This research is that transgenic engineering tomato industrialization extraction astaxanthin is established
Technical foundation is determined.
Brief description of the drawings：
Influence of Fig. 1 difference entrainers to astaxanthin recovery rate；
Influence of Fig. 2 extraction temperatures to astaxanthin recovery rate；
Influence of Fig. 3 extracting pressures to astaxanthin recovery rate；
Influence of Fig. 4 extraction times to astaxanthin recovery rate；
Fig. 5 transgenic engineering tamato fruit carotenoid chromatograms.
Embodiment：
Below in conjunction with the accompanying drawings, further illustrated with embodiments of the invention the present invention essentiality content, but not with
This limits the present invention.
Embodiment 1：
Material and method
Material and facility：
Absolute ethyl alcohol, 75% ethanol, ethyl acetate, acetone, n-hexane is that domestic analysis is pure；Isopropanol, acetonitrile, methanol
For German Fisher chromatographically pures；Supercritical carbon dioxide extraction device, U.S. Waters (TharSFE)；Ultra high efficiency liquid phase
Chromatograph, Anjelen Sci. ＆ Tech. Inc of the U.S., 1290Infinity；Chromatographic column, Anjelen Sci. ＆ Tech. Inc of the U.S.,
Eclipse plus C18 RRHD 1.8μm；Rotary Evaporators, Tokyo physics and chemistry, EYELE N-1100；Assay balance, Beijing match
Many Li Si instrument systems Co., Ltds, TP-213.
Tamato fruit dry powder：(yellow pretty tide etc. (Huang JC etc., 2013) pass through gene engineering method to astaxanthin engineering tomato
To screen and be separated in green alga can be catalyzed the assimilation enzyme and hydroxylation of beta carotene and luteole into astaxanthin in plant cell
Enzyme gene is transferred in wild-type tomatoes plant (cv.UC82B), and obtained transgene tomato is (on May 14th, 2014 in China
Microbiological Culture Collection administration committee common micro-organisms center preservation, preserving number is：CGMCC No 9223, Classification And Nomenclature：
Red No. 1 transgene tomato of shrimp.) F4 tomato dry powder, sieve 200 mesh.
Method：
Supercritical carbon dioxide extraction method：Take the astaxanthin engineering tamato fruit dry powder sieved in advance every time
200g, regulating system temperature and pressure to setting value, the temperature and pressure of kettle to be extracted are reached after setting value and stabilization, carry out 1h
Static extracting；Static extracting opens constant-flux pump after terminating, and entrainer is transported into equipment with 1.0g/min, entrainer
Enter extraction kettle with the fluid-mixing of carbon dioxide, start dynamic extraction.This research will to extracting pressure, extraction temperature,
Entrainer, extraction time are investigated, and optimum extraction condition is determined according to the recovery rate of astaxanthin, when investigating a parameter,
Set other specification constant.
Astaxanthin method for saponification：Astaxanthin part in Transgenic tomato fruit is present in the form of astaxanthin ester,
Therefore need that astaxanthin ester is completely converted into free state astaxanthin using saponification means.Specific method is：Take 1ml overcritical two
Astaxanthin extract obtained by carbon oxide fluid extracting process, is concentrated using concentrating instrument and done, be dissolved in 1ml ether, add 1ml KOH
MeOH is mixed, and 15min is reacted in 0 DEG C of dark surrounds, is added 2ml 10%NaCl and is mixed, 2000g centrifugation 2min, removes aqueous phase,
Washed twice with 2ml 10%NaCl, be dissolved in loading after 1ml acetone solns, suction filtration and determine pigment content.
Astaxanthin, lycopene, bata-carotene quantitative approach it is as follows：Accurately weigh astaxanthin, lycopene, β carrots
With acetone solution and 100ml is settled to after each 5mg mixing of plain standard items, takes 1ml, 5ml, 10ml mixed dissolution liquid is distinguished with acetone
50ml is settled to, 1ug/ml is made, 5ug/ml, 10ug/ml standard items aggregate samples make standard curve, meter using UPLC loadings
Calculate astaxanthin, lycopene, the content of bata-carotene.UPLC methods：Flow velocity：1ml/min；Sample size：5ul；0-1.0min：
Water：20%, acetonitrile：60%, isopropanol：5%, methanol：15%；1.00-2.00min：Water：0%, acetonitrile：80%, isopropanol：
5%, methanol：15%；2.00-8.00min：Water：0%, acetonitrile：80%, isopropanol：5%, methanol：15%；
As a result with analysis
Influence of the different entrainers to astaxanthin recovery rate：
During supercritical carbon dioxide extraction, when investigating the influence of entrainer, selected other specification is：Extraction
35 DEG C of temperature, extracting pressure 35MPa, extraction time 3h.50% ethanol, 75% ethanol, absolute ethyl alcohol, acetone, acetic acid are used respectively
Ethyl ester is tested as entrainer.Astaxanthin extraction rate is determined, as a result such as Fig. 1.
As seen from Figure 1, during with absolute ethyl alcohol, acetone, ethyl acetate as entrainer, Astaxanthin extraction rate compared with
It is high.This explanation, in the supercritical state, the polarity of CO 2 fluid is very small, and the addition of entrainer, particularly highly polar
Entrainer, be conducive to astaxanthin to be dissolved in supercritical carbon dioxide fluid.But acetone, ethyl acetate are organic solvents, residual
Make things difficult for remove completely, and absolute ethyl alcohol not only safety, and cost is low, recovery rate is of a relatively high, therefore can select anhydrous
Ethanol is used as optimal entrainer.
Influence of the extraction temperature to astaxanthin recovery rate：
During supercritical carbon dioxide extraction, when investigating the influence of temperature, selected other specification is：Extraction pressure
Power 35MPa, extraction time 3h, entrainer absolute ethyl alcohol, in 30 DEG C of this experiment condition design temperature, 35 DEG C, 40 DEG C, 45 DEG C, 50
DEG C, 55 DEG C, 60 DEG C.Astaxanthin extraction rate is determined, as a result such as Fig. 2.
As seen from Figure 2, the recovery rate of astaxanthin is raised with the rise of temperature, and temperature reaches maximum when being 50 DEG C,
Afterwards, recovery rate is reduced with the rise of temperature.This may be because when temperature rise, vapour pressure increase makes astaxanthin face super
Solubility increase in boundary's CO 2 fluid, but when temperature continues to raise because free state astaxanthin holds in high temperature environments
Easily by thermal decomposition so as to cause recovery rate to reduce.Therefore, the optimum temperature of supercritical carbon dioxide extraction astaxanthin is 50
℃。
Influence of the extracting pressure to astaxanthin recovery rate：
During supercritical carbon dioxide extraction, when investigating the influence of pressure, selecting other experiment parameters is：Temperature
50 DEG C of degree, entrainer absolute ethyl alcohol, extraction time 3h, 10 DEG C of pressure, 15 DEG C, 20 DEG C, 25 DEG C, 30 are set in this experiment condition
DEG C, 35 DEG C, 40 DEG C.Astaxanthin extraction rate is determined, as a result such as Fig. 3.
As seen from Figure 3, the recovery rate of astaxanthin increases with increasing for extracting pressure, during pressure 35MPa, extracts
Rate is 90%, during pressure 40MPa, and recovery rate is 92%.This explanation increases when pressure, fluid density increase, causes extract to exist
Solubility in supercritical carbon dioxide fluid is improved, and causes the rate increase of extraction Astaxanthin extraction.It is more favourable although pressure is higher
In the raising of recovery rate, but due to experimental facilities limitation, and in production application, the too high of pressure can cause equipment life to be dropped
It is low, increase operation cost, therefore use 35MPa for optimal extracting pressure.
Influence of the extraction time to astaxanthin recovery rate：
During supercritical carbon dioxide extraction, when investigating the influence of extraction time, other experiment parameters are selected
For：50 DEG C of extraction temperature, extracting pressure 35MPa, entrainer is absolute ethyl alcohol；1.0h, 1.5h, 2.0h, 2.5h are extracted respectively,
3.0h, 3.5h, 4.0h.Astaxanthin extraction rate is determined, as a result such as Fig. 4.
As seen from Figure 4, the recovery rate of astaxanthin increases with the increase of extraction time, and extraction rate reached is arrived during 2.5h
Maximum, in 4h, recovery rate drastically declines, this be probably due to thermal sensitivity astaxanthin long-time be in 50 DEG C of extraction temperature,
Under conditions of 35MPa, astaxanthin is caused by thermal decomposition.Therefore 2.5h is optimum extraction time.
Carotenoid content comparative analysis in tamato fruit：
During supercritical carbon dioxide extraction, in carotenoid content in investigating fruit and recovery rate, if
Determine the optimum extraction condition that experiment parameter is astaxanthin：50 DEG C of extraction temperature, extracting pressure 35MPa, absolute ethyl alcohol extraction 2h, point
Ji Suan not astaxanthin, lycopene, the content of bata-carotene and recovery rate.
From Fig. 5, table 1 as can be seen that not only containing astaxanthin, also lycopene and β in transgenic engineering tamato fruit
Carrotene.The recovery rate of astaxanthin is 95%, and content is 1.90mg/g dry weights, and lycopene is 82%, and content is
0.74mg/g dry weights, the recovery rate of bata-carotene is 89%, and content is 0.85mg/g dry weights.
The transgenic engineering tamato fruit carotenoid content of table 1
The best-of-breed technology scheme of the present invention：
Take the tamato fruit dry powder 200g sieved in advance, regulating system temperature is to 50 DEG C, extracting pressure 35MPa is to be extracted
The temperature and pressure of kettle are reached after setting value and stabilization, carry out 1h static extracting；Static extracting opens constant-flux pump after terminating, will
Absolute ethyl alcohol is transported into equipment, and the fluid-mixing of absolute ethyl alcohol and carbon dioxide enters extraction kettle, starts dynamic
Extraction, dynamic extraction time is 2h, and the recovery rate of astaxanthin is 95%.While astaxanthin being extracted under this extraction conditions, this
Invention also obtains lycopene, the bata-carotene of high value simultaneously, and its recovery rate is respectively 82% and 89%.
Claims (7)
1. the supercritical carbon dioxide extraction method of astaxanthin in transgenic engineering tamato fruit, it is characterised in that this method
The astaxanthin engineering tamato fruit dry powder sieved in advance including taking, regulating system temperature and pressure to setting value, kettle to be extracted
Temperature and pressure are reached after setting value and stabilization, carry out 1h static extracting；Static extracting opens constant-flux pump after terminating, with
Entrainer is transported into equipment by 1.0g/min, and the fluid-mixing of entrainer and carbon dioxide enters extraction kettle, opens
Beginning dynamic extraction, then carries out saponification.
2. the supercritical carbon dioxide extraction side of astaxanthin in transgenic engineering tamato fruit as claimed in claim 1
Method, it is characterised in that astaxanthin ester is further completely converted into free state astaxanthin by this method using saponification means, takes shrimp blue or green
Plain engineering tomato extract carries out saponification, takes 1ml extract solutions, is concentrated and done using concentrating instrument, be dissolved in 1ml ether, adds 1ml KOH
MeOH is mixed, and 15min is reacted in 0 DEG C of dark surrounds, is added 2ml 10%NaCl and is mixed, 2000g centrifugation 2min, removes aqueous phase,
Washed twice with 2ml 10%NaCl, be dissolved in loading after 1ml acetone solns, suction filtration and determine pigment content.
3. the supercritical carbon dioxide extraction side of astaxanthin in transgenic engineering tamato fruit as claimed in claim 1
Method, it is characterised in that take the tamato fruit dry powder sieved in advance, regulating system temperature is to 50 DEG C, and extracting pressure 35MPa is to be extracted
The temperature and pressure of kettle are reached after setting value and stabilization, carry out 1h static extracting；Static extracting opens constant-flux pump after terminating, will
Absolute ethyl alcohol is transported into equipment, and the fluid-mixing of absolute ethyl alcohol and carbon dioxide enters extraction kettle, starts dynamic
Extraction, dynamic extraction time is 2h.
4. the carotenoid supercritical carbon dioxide extraction method in transgenic engineering tamato fruit, it is characterised in that should
Method includes taking the tamato fruit dry powder that sieves in advance, and regulating system temperature is to 50 DEG C, extracting pressure 35MPa, kettle to be extracted
Temperature and pressure are reached after setting value and stabilization, carry out 1h static extracting；Static extracting opens constant-flux pump after terminating, will be anhydrous
Ethanol is transported into equipment, and the fluid-mixing of absolute ethyl alcohol and carbon dioxide enters extraction kettle, starts dynamic extraction,
Dynamic extraction time is 2h.
5. the carotenoid supercritical carbon dioxide extraction in transgenic engineering tamato fruit as claimed in claim 4
Method, it is characterised in that this method is further quantitative determined to carotenoid content, described carotenoid is blue or green for shrimp
Element, lycopene, bata-carotene, its method for quantitatively determining are used：Accurately weigh astaxanthin, lycopene, bata-carotene standard
With acetone solution and 100ml is settled to after each 5mg of product mixing, takes 1ml, 5ml, 10ml mixed dissolutions liquid is settled to respectively with acetone
50ml, is made 1ug/ml, and 5ug/ml, 10ug/ml standard items aggregate samples make standard curve using UPLC loadings, calculate shrimp blue or green
Element, lycopene, the content of bata-carotene, described UPLC methods are：Flow velocity：1ml/min；Sample size：5ul；0-1.0min：
Water：20%, acetonitrile：60%, isopropanol：5%, methanol：15%；1.00-2.00min：Water：0%, acetonitrile：80%, isopropanol：
5%, methanol：15%；2.00-8.00min：Water：0%, acetonitrile：80%, isopropanol：5%, methanol：15%.
6. the supercritical carbon dioxide extraction method of lycopene in transgenic engineering tamato fruit, it is characterised in that the party
Method includes taking the tamato fruit dry powder sieved in advance, regulating system temperature to 50 DEG C, extracting pressure 35MPa, the temperature of kettle to be extracted
Degree and pressure are reached after setting value and stabilization, carry out 1h static extracting；Static extracting opens constant-flux pump after terminating, by anhydrous second
Alcohol is transported into equipment, and the fluid-mixing of absolute ethyl alcohol and carbon dioxide enters extraction kettle, starts dynamic extraction, moves
State extraction time is 2h.
7. the supercritical carbon dioxide extraction method of bata-carotene in transgenic engineering tamato fruit, it is characterised in that the party
Method includes taking the tamato fruit dry powder sieved in advance, regulating system temperature to 50 DEG C, extracting pressure 35MPa, the temperature of kettle to be extracted
Degree and pressure are reached after setting value and stabilization, carry out 1h static extracting；Static extracting opens constant-flux pump after terminating, by anhydrous second
Alcohol is transported into equipment, and the fluid-mixing of absolute ethyl alcohol and carbon dioxide enters extraction kettle, starts dynamic extraction, moves
State extraction time is 2h.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108929256A (en) * | 2018-07-03 | 2018-12-04 | 华南理工大学 | Broken wall lactobacillus supercritical CO2The static method for extracting astaxanthin with dynamic cooperation |
| KR20210053166A (en) * | 2019-10-29 | 2021-05-11 | 주식회사 내추럴바이오트리 | Method For Making Astaxanthin Monoester |
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| KR102423638B1 (en) * | 2019-10-29 | 2022-07-22 | 주식회사 내추럴바이오트리 | Method For Making Astaxanthin Monoester |
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