CN107228838B - A kind of cell elasticity measuring system based on optical fiber - Google Patents
A kind of cell elasticity measuring system based on optical fiber Download PDFInfo
- Publication number
- CN107228838B CN107228838B CN201710422054.0A CN201710422054A CN107228838B CN 107228838 B CN107228838 B CN 107228838B CN 201710422054 A CN201710422054 A CN 201710422054A CN 107228838 B CN107228838 B CN 107228838B
- Authority
- CN
- China
- Prior art keywords
- optical fiber
- driving motor
- fbg
- demodulator
- protective case
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 239000013307 optical fiber Substances 0.000 title claims abstract description 78
- 230000001681 protective effect Effects 0.000 claims abstract description 27
- 238000006073 displacement reaction Methods 0.000 claims abstract description 23
- 238000005259 measurement Methods 0.000 claims abstract description 19
- 230000003287 optical effect Effects 0.000 claims abstract description 10
- 239000011324 bead Substances 0.000 claims description 9
- 238000007373 indentation Methods 0.000 abstract description 7
- 239000000835 fiber Substances 0.000 abstract description 4
- 238000000034 method Methods 0.000 description 20
- 210000001519 tissue Anatomy 0.000 description 10
- 238000006243 chemical reaction Methods 0.000 description 6
- 210000004872 soft tissue Anatomy 0.000 description 6
- 201000010099 disease Diseases 0.000 description 5
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 5
- 210000003128 head Anatomy 0.000 description 5
- 238000003384 imaging method Methods 0.000 description 4
- 238000010586 diagram Methods 0.000 description 3
- 210000000056 organ Anatomy 0.000 description 3
- 230000001575 pathological effect Effects 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 238000002052 colonoscopy Methods 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 238000013399 early diagnosis Methods 0.000 description 2
- 210000003205 muscle Anatomy 0.000 description 2
- 238000005303 weighing Methods 0.000 description 2
- 201000001320 Atherosclerosis Diseases 0.000 description 1
- 201000003883 Cystic fibrosis Diseases 0.000 description 1
- 206010016654 Fibrosis Diseases 0.000 description 1
- 238000005481 NMR spectroscopy Methods 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 238000003339 best practice Methods 0.000 description 1
- 210000004204 blood vessel Anatomy 0.000 description 1
- 210000000988 bone and bone Anatomy 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 230000007882 cirrhosis Effects 0.000 description 1
- 208000019425 cirrhosis of liver Diseases 0.000 description 1
- 210000004087 cornea Anatomy 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 230000004064 dysfunction Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000002091 elastography Methods 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000000691 measurement method Methods 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 210000003491 skin Anatomy 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 210000002435 tendon Anatomy 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/41—Refractivity; Phase-affecting properties, e.g. optical path length
Landscapes
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Strength Of Materials By Application Of Mechanical Stress (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Abstract
The cell elasticity measuring system based on optical fiber that the present invention relates to a kind of; the system includes optical fiber, protective case, driving motor, (FBG) demodulator and processor; driving motor is fixed in protective case; optical fibre is arranged in protective case and optical fiber one end exposes outside protective case; driving motor connects optical fiber; optical fiber connects (FBG) demodulator, and driving motor and (FBG) demodulator are all connected with processor；Driving motor driving optical fiber moves in protective case; inside exposed optical fiber end indentation cell sample; (FBG) demodulator obtains optical fiber optical signal and is demodulated into voltage signal; the corresponding voltage signal that processor records driving motor driving fibre movement displacement and (FBG) demodulator obtains, processor is displaced to fibre movement and voltage signal is handled to obtain cell sample elasticity size.Compared with prior art, measurement accuracy of the present invention is high, and measurement result is more accurate.
Description
Technical field
The present invention relates to a kind of cell elasticity measuring systems, measure system more particularly, to a kind of cell elasticity based on optical fiber
System.
Background technique
In addition to bone and tooth, organism it is every other tissue such as: organ, muscle, blood vessel, muscle, tendon, cornea, skin,
Even cell is biologic soft tissue.When the elasticity of biologic soft tissue changes, generally can be shown that its dysfunction or
Disease has occurred and that, existing research shows that some diseases such as malignant tumour, cirrhosis, atherosclerosis, cystic fibrosis etc. are equal
It will lead to the Flexible change of biologic soft tissue.Therefore, the elasticity measurement of biologic soft tissue has the early diagnosis of disease non-
Normal important role.In recent years, elastography is carried out extensively to measure the elasticity of biologic soft tissue as Diseases diagnosis
Foundation, such as nuclear magnetic resonance elastogram, ultrasonic elastograph imaging be clinically widely applied.The equal energy of these imaging methods
Observational measurement is carried out to the elasticity of biologic soft tissue: when pathological tissues and normal bio tissue elasticity are there are when magnitude differences,
It can be realized the examination to health and pathological tissues with these imaging techniques.But these methods are qualitative method, work as biological tissue
When small change occurs for elasticity, it can not be judged by these methods, therefore existing elastograph imaging method can not be used as disease
Early diagnosis scheme.For that can make elastogram that there is broader pathological examination application prospect, propose that biological tissue elasticity is fixed
Quantity measuring method is very necessary.
Existing quantitative detection biological tissue elasticity method mainly has indentation method and pulling method.Indentation method is because of experimental provision system
Make relatively easy, easy to operate and be widely used, indentation method experimental provision is as shown in Figure 1.Device is mainly by servo motor 11
It is formed with this two parts of laser displacement gauge 12.Sample 15 is placed on sample table 16, and cantilever beam 13 is placed in 15 top of sample, servo
Motor 11 is mainly used to control the decline displacement of cantilever beam 13, makes in 14 pressing in sample 15 of bead before cantilever beam 13, simultaneously
Read the decline displacement of servo motor 11.After 15 surface of sample is encountered on 14 surface of bead, cantilever beam 13 is instead made by sample 15
It is firmly bent, 12 incident laser of laser displacement gauge is got to 13 back side of cantilever beam and reflected, by detecting reflection laser point position
13 test samples 15 of cantilever beam can be derived to its active force, and by active force read-record.Record the lower demotion of servo motor 11
It moves with sample 15 to the numerical value of both active forces of cantilever beam 13, the bullet of sample can be calculated according to Hertz-Sneddon model
Property.This method accuracy is high, and test speed is fast, and is quantitative approach.Be also existing quantitative measurment elasticity best practice it
One.
Although this method is industrially widely used, when testing Biosample, limitation is very big.First is that because
Its measurement accuracy is highly dependent on the precision of servo motor 11 and laser displacement gauge 12, makes that impression instrument with high accuracy must be made
Higher cost, and be difficult to minimize.Second is its elasticity that can only measure organism surface: sample 15 need to be placed in sample
It is measured on platform 16, therefore organism surface position can only be carried out can only to carry out in vitro internal etc. in bulk measurement
Measurement.Therefore with a distance from clinical application also some.
Summary of the invention
It is an object of the present invention to overcome the above-mentioned drawbacks of the prior art and provide a kind of based on the thin of optical fiber
Born of the same parents' elasticity measurement system.
The purpose of the present invention can be achieved through the following technical solutions:
A kind of cell elasticity measuring system based on optical fiber, the system include optical fiber, protective case, driving motor, (FBG) demodulator
And processor, the driving motor are fixed in protective case, the optical fibre is arranged in protective case and optical fiber one end
The protective case is exposed outside, the driving motor connects optical fiber, and the optical fiber connects (FBG) demodulator, the driving motor
The processor is all connected with (FBG) demodulator；
Driving motor driving optical fiber moves in protective case, inside exposed optical fiber end indentation cell sample, (FBG) demodulator
It obtains optical fiber optical signal and is demodulated into voltage signal, processor record driving motor driving fibre movement displacement and (FBG) demodulator obtain
The corresponding voltage signal taken, processor is displaced fibre movement and voltage signal is handled to obtain cell sample elasticity greatly
It is small.
The driving motor is stepper motor.
Exposed optical fiber end connects a dome-type rigidity bead, and when carrying out cell elasticity measurement, the rigidity is small
Ball spherical surface is pressed into inside cell sample.
The processor is computer.
A method of cell elasticity measurement being carried out using the cell elasticity measuring system based on optical fiber, this method includes such as
Lower step:
(1) optical fiber end for exposing outside protective case is directed at cell sample by fixed protective case；
(2) processor control driving motor work, driving motor drive inside optical fiber indentation cell sample；
(3) in driving motor motion process, in different moving displacements, (FBG) demodulator obtains optical fiber optical signal and demodulates
For voltage signal, processor records the corresponding voltage signal that moving displacement and (FBG) demodulator obtain；
(4) processor control driving motor moves in opposite directions, and optical fiber is separated with cell sample；
(5) cell sample bullet is calculated according to the corresponding voltage signal that moving displacement and (FBG) demodulator obtain in processor
Property size.
Step (5) specifically: processor converts cell sample to the anti-work of optical fiber for the voltage signal that (FBG) demodulator obtains
Firmly, and then processor draws reaction force-moving displacement curve, finally, processor is by reaction force-moving displacement curve generation
Enter the elastic size that cell sample is calculated in Hertz-Sneddon model.
Compared with prior art, the present invention has the advantage that
(1) cell elasticity measuring system of the present invention accepts rear optical fibre refractivity variation using optical fiber and pushes away cell sample to counter
Reaction force, measurement accuracy is high, and measurement result is more accurate；
(2) cell elasticity measuring system of the present invention can realize miniaturization, for the elasticity measurement of biological intracorporeal organ, tissue
It is same effective, it is only necessary to which that importing optical fiber in human body by gastroscope, colonoscopy etc. can measure；
(3) step motor control of the present invention is accurate, improves measurement accuracy；
(4) the configuration of the present invention is simple, low manufacture cost.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of indentation method experimental provision；
Fig. 2 is that the present invention is based on the structural schematic diagrams of the cell elasticity measuring system of optical fiber；
Fig. 3 is that the present invention is based on the flow diagrams of the cell elasticity measurement method of optical fiber.
In figure, 11 be servo motor, and 12 be laser displacement gauge, and 13 be cantilever beam, and 14 be bead, and 15 be sample, and 16 be examination
Sample platform, 21 be optical fiber, and 22 be protective case, and 23 be driving motor, and 24 be (FBG) demodulator, and 25 be processor.
Specific embodiment
The present invention is described in detail with specific embodiment below in conjunction with the accompanying drawings.
Embodiment
As shown in Fig. 2, a kind of cell elasticity measuring system based on optical fiber, which includes optical fiber 21, protective case 22, drives
Dynamic motor 23, (FBG) demodulator 24 and processor 25, driving motor 23 are fixed in protective case 22, and optical fiber 21 is movably arranged on protective case
In 22 and 21 one end of optical fiber exposes outside protective case 22, and the optical fiber 21 for exposing outside protective case 22 forms pressure head, and driving motor 23 connects
Optical fiber 21, optical fiber 21 connect (FBG) demodulator 24, and driving motor 23 and (FBG) demodulator 24 are all connected with processor 25；
Driving motor 23 drives optical fiber 21 to move in protective case 22, and exposed 21 end of optical fiber (i.e. pressure head) is pressed into cell
Inside sample, (FBG) demodulator 24 obtains 21 optical signal of optical fiber and is demodulated into voltage signal, and processor 25 records driving motor 23 and drives
The corresponding voltage signal that 21 moving displacement of optical fiber and (FBG) demodulator 24 obtain, processor 25 is to 21 moving displacement of optical fiber and voltage
Signal is handled to obtain cell sample elasticity size.Driving motor 23 is stepper motor.Processor 25 is computer.Exposed
21 end of optical fiber connects a dome-type rigidity bead, and when carrying out cell elasticity measurement, rigid bead spherical surface is pressed into cell sample
Inside, when being not provided with ball-type rigidity bead, the calculation formula of its elasticity needs to correct in processor 25.
As shown in figure 3, a kind of method that cell elasticity measurement is carried out using the cell elasticity measuring system based on optical fiber, it should
Method includes the following steps:
(1) fixed protective case 22, will expose outside the 21 end alignment cell sample of optical fiber of protective case 22；
(2) processor 25 controls driving motor 23 and works, and driving motor 23 drives optical fiber 21 to be pressed into inside cell sample；
(3) in 23 motion process of driving motor, in different moving displacements, (FBG) demodulator 24 obtains 21 optical signal of optical fiber
And it is demodulated into voltage signal, processor 25 records the corresponding voltage signal that moving displacement and (FBG) demodulator 24 obtain；
(4) processor 25 controls driving motor 23 and moves in opposite directions, and optical fiber 21 is separated with cell sample；
(5) cell examination is calculated according to the corresponding voltage signal that moving displacement and (FBG) demodulator 24 obtain in processor 25
Sample elasticity size.
Step (5) specifically: processor 25 converts cell sample to optical fiber 21 for the voltage signal that (FBG) demodulator 24 obtains
Reaction force, and then processor 25 draws reaction force-moving displacement curve, finally, processor 25 is by reaction force-movement
Displacement curve substitutes into the elastic size that cell sample is calculated in Hertz-Sneddon model.
When first time using the equipment, first equipment should be calibrated.It need to prepare a balance.By protective case 22 along height
Degree direction is fixed, and makes its pressure head part close to balance weighing apparatus surface.Make optical fiber 21 and front with computer control micro-step motor
Pressure head is moved down and is pressed balance weighing apparatus (general depth is micron to millimeter order), and the light in the process receiving optical fiber 21 is believed
Number computer is fed back to, while the reading on recording balance, it is a pair of to can be obtained optical fiber 21 receives between optical signal and feedback force one
It should be related to.
The working principle of the invention: being 21 reflecting surface of optical fiber at 21 end rear of optical fiber (i.e. pressure head rear), according to optical fiber
21 mechanical characteristic, light to 21 end face of optical fiber and are reflected by built-in laser light incident, after by the effect of top power, optical fiber 21
Inner refractive index will change.By not by biological tissue's feedback force when obtained optical signal be reference light, obtained by feedback force
To optical signal to subtract the signal of reference light be exactly by refraction index changing bring optical path difference, which can be obtained by calculation
The change of refractive index simultaneously counter releases feedback force.By the displacement signal of the feedback force and stepper motor by numerical value handle can obtain it is biological
Group is woven in the elasticity of measurement point.It is equally effective for the elasticity measurement of biological intracorporeal organ, tissue, it is only necessary to which that optical fiber 21 is passed through into stomach
Mirror, colonoscopy etc. are imported in human body and be can measure.
Claims (4)
1. a kind of cell elasticity measuring system based on optical fiber, which is characterized in that the system includes optical fiber (21), protective case
(22), driving motor (23), (FBG) demodulator (24) and processor (25), the driving motor (23) are fixed on protective case (22)
Interior, the optical fiber (21) is movably arranged in protective case (22) and optical fiber (21) one end and exposes outside the protective case (22),
The driving motor (23) connects optical fiber (21), and the optical fiber (21) connects (FBG) demodulator (24), the driving motor
(23) and (FBG) demodulator (24) is all connected with the processor (25)；
Driving motor (23) drives optical fiber (21) movement in protective case (22), and exposed optical fiber (21) end is pressed into cell sample
Inside, (FBG) demodulator (24) obtain optical fiber (21) optical signal and are demodulated into voltage signal, and processor (25) records driving motor (23)
The corresponding voltage signal for driving optical fiber (21) moving displacement and (FBG) demodulator (24) to obtain, processor (25) transport optical fiber (21)
Dynamic displacement and voltage signal are handled to obtain cell sample elasticity size.
2. a kind of cell elasticity measuring system based on optical fiber according to claim 1, which is characterized in that the driving
Motor (23) is stepper motor.
3. a kind of cell elasticity measuring system based on optical fiber according to claim 1, which is characterized in that exposed optical fiber
(21) end connects a dome-type rigidity bead, and when carrying out cell elasticity measurement, the rigid bead spherical surface is pressed into cell
Inside sample.
4. a kind of cell elasticity measuring system based on optical fiber according to claim 1, which is characterized in that the processing
Device (25) is computer.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710422054.0A CN107228838B (en) | 2017-06-07 | 2017-06-07 | A kind of cell elasticity measuring system based on optical fiber |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710422054.0A CN107228838B (en) | 2017-06-07 | 2017-06-07 | A kind of cell elasticity measuring system based on optical fiber |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107228838A CN107228838A (en) | 2017-10-03 |
| CN107228838B true CN107228838B (en) | 2019-10-01 |
Family
ID=59935806
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710422054.0A Expired - Fee Related CN107228838B (en) | 2017-06-07 | 2017-06-07 | A kind of cell elasticity measuring system based on optical fiber |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107228838B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111859739B (en) * | 2020-07-02 | 2022-09-20 | 大连理工大学 | Correction method for fitting cell elastic modulus by Sneddon model |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6442305B1 (en) * | 1999-12-21 | 2002-08-27 | Sabeus Photonics, Inc. | Method for altering the refractive index of optical fibers using stress |
| CN101634629A (en) * | 2009-08-27 | 2010-01-27 | 电子科技大学 | Cancer cell detection method based on optical stretching and optical fiber interference sensing technology |
| US9241693B2 (en) * | 2010-07-20 | 2016-01-26 | The Johns Hopkins University | Interferometric force sensor for surgical instruments |
| CN103149177B (en) * | 2013-01-14 | 2014-12-17 | 天津先阳科技发展有限公司 | Device and method for detecting biological tissue of pressure modulation near infrared spectrum |
| CN105078412B (en) * | 2015-07-07 | 2018-08-31 | 上海理工大学 | Tissue elasticity analysis method and device based on optoacoustic spectrum analysis |
-
2017
- 2017-06-07 CN CN201710422054.0A patent/CN107228838B/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CN107228838A (en) | 2017-10-03 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Bandari et al. | Tactile sensors for minimally invasive surgery: A review of the state-of-the-art, applications, and perspectives | |
| JP3575025B2 (en) | Pulse wave detector and pulsation detector | |
| Polygerinos et al. | Novel miniature MRI-compatible fiber-optic force sensor for cardiac catheterization procedures | |
| CN104825121B (en) | Endoscopic OCT (Optical Coherence Tomography) miniature probe, OCT imaging system and use method | |
| CN106199078B (en) | A kind of quick accurate Characterization method of active somatic cell surface topography atomic force microscope | |
| CN103263250B (en) | Superficial tissue elastography system based on piezoelectric cantilever beam contact vibration | |
| Jones | Biomedical sensors | |
| CN103271720A (en) | Pulse instrument with adjustable measuring pressure head position | |
| CN108445077B (en) | Optical elasticity test method | |
| CN104794740A (en) | Method and system for processing OCT (Optical Coherence Tomography) signal by using general purpose graphic processing unit | |
| CN103549937B (en) | Tissue-swelling measurement instrument and control method thereof | |
| Uzun et al. | Optical force sensor with enhanced resolution for MRI guided biopsy | |
| CN103040429A (en) | Optical image detection device for oral cavity and imaging method | |
| CN204636278U (en) | Inner peeping type OCT miniature probe and OCT imaging system | |
| CN106510644B (en) | Medical optical coherence chromatographic imaging two dimension scan forward probe based on fiber optic bundle | |
| WO2009067573A2 (en) | Apparatus and method for measuring the dimensions of the palpable surface of the prostate | |
| CN204698494U (en) | The optical clock module used in scanning imaging system is peeped in OCT | |
| CN107228838B (en) | A kind of cell elasticity measuring system based on optical fiber | |
| US20110125004A1 (en) | Analysis by photo acoustic displacement and interferometryl | |
| CN113520617A (en) | Passive three-dimensional force sensing measuring head and optical force sensor | |
| CN203153692U (en) | Optical image detecting device for mouth cavity | |
| CN212592088U (en) | OCT imaging system | |
| CN212300424U (en) | Optical fiber probe combining OCT imaging and pressure sensing | |
| US8876735B2 (en) | Apparatus and methods for identifying a tissue inside a living body | |
| CN110037711B (en) | Blood glucose optoacoustic accurate positioning detection device and method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20191001 |