CN107224604A

CN107224604A - A kind of preparation method of chitosan-based compound sponge as wound dressing

Info

Publication number: CN107224604A
Application number: CN201710468546.3A
Authority: CN
Inventors: 陈西广; 胡世浩; 程晓杰
Original assignee: Ocean University of China
Current assignee: Ocean University of China
Priority date: 2017-06-20
Filing date: 2017-06-20
Publication date: 2017-10-03

Abstract

The present invention devises a kind of using chitosan and hydroxyl butyl chitosan as the preparation method of the compound sponge of raw material.By the way that chitosan-acetic acid solution dialysis is obtained into weakly acidic pH chitosan acetate solution, then temperature is controlled it is mixed by a certain percentage with hydroxyl butyl chitosan solution, it is lyophilized to obtain compound sponge.Present invention incorporates both advantages, both shortcomings are compensate for, hydrophily is strong, and high mechanical strength, good antimicrobial effect can be effectively facilitated wound healing, and have good patient dependence, there is the great potential as excellent wound dressing.

Description

A kind of preparation method of chitosan-based compound sponge as wound dressing

Technical field

The present invention relates to a kind of preparation method of the chitosan-based compound sponge as wound dressing, belong to bio-medical Material Field.

Background technology

Skin is the maximum organ of human body, covers whole people's external surface, is keeping body internal and external equilibrium and is preventing micro- life Played an important role in thing invasion, skin is also human body most easily damaged organ.There is millions of people by many mechanical factors every year Wound, such as motion, operation, traffic accident, war, earthquake etc., are the reason for skin injury are common；Secondly there are a large amount of diseases Skin injury that disease is caused etc..It can cause wound infection as nursed not in time after skin damage, or even cause necrosis, seriously When threat to life.Therefore, dressing is needed to protect when wound occurs for skin, to prevent bacterial invasion.A kind of good wound is applied The microenvironment that material can also provide propagation for cell promotes the regeneration of skin and the reparation of quick health.

Chitosan is unique positively charged alkaline polysaccharide in nature, is exempted from hemostasis, antibacterial, anticancer, lipid-loweringing, enhancing Different physiological roles such as epidemic disease, and nontoxic, non-stimulated, degradable, with good biocompatibility, be widely used in medicine and Bio-medical dressing field.Chitosan can be crosslinked with erythrocyte surface and make erythrocyte aggregation, and this characteristic makes chitosan sponge As fast and effectively wound hemostasis dressing.However, chitosan sponge is water insoluble, water imbibition is poor, lacks enough water conservation energy Power, it is difficult to absorb wound hydrops and keep wound moist, and when the dressing is changed, patient compliance is poor.Chitosan is carried out The obtained hydroxyl butyl chitosan of hydroxyl butylation transformation, has spongy advantage, can absorbing wound exudate, be swelled and insoluble Yu Shui, to no skin irritation, the convenient without pain of dressing, moreover it is possible to good moist environment is provided for wound, is conducive to skin to create The growth reparation in face, is a kind of new chitosan class wound dressing；But hydroxyl butyl chitosan sponge bad mechanical strength, antibacterial effect Fruit is not obvious, is its defect.

In order to make up existing chitosan sponge and existing hydroxyl butyl chitosan sponge as lacking for existing in terms of wound dressing Point and deficiency, the present invention have developed a kind of new chitosan-based compound sponge as wound dressing.

The content of the invention

It is an object of the invention to provide a kind of preparation method of the chitosan-based compound sponge as wound dressing, with reference to list The advantage of pure chitosan sponge dressing and simple hydroxyl butyl chitosan sponge dressing, both is combined to overcome both as wound The deficiency of mouth dressing.

The present invention is molten by using chitosan as raw material, preparing weakly acidic pH chitosan acetate solution and hydroxyl butyl chitosan respectively Liquid, then chitosan-based compound Sponge of wound dressing is prepared by mixing into varing proportions.

Chitosan-based compound sponge dressing acid-base property weakly acidic pH prepared by the present invention, eliminates acetic acid and low ph value to skin The stimulation of the surface of a wound.

Chitosan-based compound sponge prepared by the present invention can absorb substantial amounts of tissue exudates, pin nutriment, keep Wound moist, and with good mechanical strength.

The effective bacteria growing inhibiting of chitosan-based compound sponge energy prepared by the present invention, promotes skin fibroblasts Growth, promotes the healing of the quick health of wound.

The operating procedure of the present invention is as follows：

Step one：The preparation of weakly acidic pH chitosan acetate solution

With 0.1%-3% acetums, then CS powder is added in acetic acid solution, stirring 1-5h makes it fully dissolve, then with steaming Distilled water is dialysed 3-6 days, until pH is 5.8-6.5.Then solution is concentrated, obtains 0.5%-2% weakly acidic pH chitosan acetate Solution.

Step 2：The preparation of hydroxyl butyl chitosan solution

Hydroxyl butyl chitosan is shredded in addition distilled water, it is dissolved at a temperature of being placed in 1 DEG C -10 DEG C, obtains 1%-5% hydroxyl fourth Base chitosan solution.

Step 3：The preparation of compound sponge

At a temperature of 1 DEG C -10 DEG C, the chitosan acetate solution that hydroxyl butyl chitosan solution and step one are obtained is with volume ratio For 5:1-1:5 ratio mixing, both sum concentration are 0.58%-4.5%, then by mixed liquor vacuum freezedrying, are answered Square sponge dressing product.

Embodiment

With reference to example, the present invention will be further described, but the present invention is not limited only to following Examples.

Embodiment 1：

The first step：In 3% acetum that 1g Chitosan powders are added to 100 mL, 1h is stirred, then is placed on dialysing in distilled water 3 days, until pH is 5.8, solution is then concentrated into 200mL, 0.5% chitosan acetate solution is obtained；Second step：Will 1 DEG C of 0.2g hydroxyl butyl chitosan is dissolved in 20 mL distilled water, obtains 1% hydroxyl butyl chitosan solution, then by hydroxyl butyl shell Glycan solution is 1 by volume with chitosan acetate solution:5 ratio uniform mixing, both sum concentration are 0.58%, most Afterwards by mixed liquor vacuum freezedrying, finished product is obtained.

Embodiment 2：

The first step：In 0.1% acetum that 1g Chitosan powders are added to 100 mL, stirring makes it fully dissolve, then is placed on Dialysed 6 days in distilled water, until pH is 6.5, solution is then concentrated into 50mL, 2% chitosan acetate solution is obtained；The Two steps：By 5g hydroxyl butyl chitosan, 10 DEG C are dissolved in 100 mL distilled water, 5% hydroxyl butyl chitosan solution are obtained, then by hydroxyl Butyl chitosan solution is with chitosan acetate solution using volume ratio as 5:1 ratio uniform mixing, both are at sum concentration 4.5%, finally by mixed liquor vacuum freezedrying, obtain finished product.

Embodiment 3：

The first step：In 1% acetum that 1g Chitosan powders are added to 100 mL, stirring 3h makes it fully dissolve, then is placed on Dialysed 4 days in distilled water, until pH is 6.0, solution is then concentrated into 100mL, 1% chitosan acetate solution is obtained；The Two steps：By 1g hydroxyl butyl chitosan, 6 DEG C are dissolved in 33.3 mL distilled water, 3% hydroxyl butyl chitosan solution are obtained, then by hydroxyl Butyl chitosan solution is with chitosan acetate solution using volume ratio as 3:1 ratio mixing, both sum concentration are 2.5%, most Afterwards by mixed liquor vacuum freezedrying, finished product is obtained.

With reference to wounds in animals experiment embodiment, further illustrate that chitosan-based compound sponge prepared by the present invention has well Promote the ability of wound healing, results icon is as follows：

Fig. 1 is the rats in vitro wound healing assay figure of different chitosan-based compound sponge dressing processing.

Claims

1. the preparation method of a kind of chitosan-based compound sponge as wound dressing, it is characterised in that be dissolved in Chitosan powder Dilute acetic acid solutions, dialysis to pH is in weakly acidic pH, is concentrated to give weakly acidic pH chitosan acetate solution.

2. secondly mixing hydroxyl butyl chitosan solution with certain proportion with chitosan acetate solution, finally mixed liquor is freezed Vacuum drying, obtains the chitosan-based compound sponge of finished product.

3. a kind of preparation method of chitosan-based compound sponge as wound dressing as described in claim 1, its feature exists It is 5.8-6.5 to be dialysed in chitosan-acetic acid solution to pH value.

4. a kind of preparation method of chitosan-based compound sponge as wound dressing as described in claim 1, its feature exists In weakly acidic pH chitosan acetate solution concentration be 0.5%-2%.

5. a kind of preparation method of chitosan-based compound sponge as wound dressing as described in claim 1, its feature exists 1 DEG C -10 DEG C of temperature control when hydroxyl butyl chitosan solution and chitosan acetate solution are mixed.

6. a kind of preparation method of chitosan-based compound sponge as wound dressing as described in claim 1, its feature exists In hydroxyl butyl chitosan solution and chitosan acetate solution ratio be 5:1-1:5.

7. a kind of preparation method of chitosan-based compound sponge as wound dressing as described in claim 1, its feature exists The concentration of both sums is 0.58%-4.5% after hydroxyl butyl chitosan solution and chitosan acetate solution are mixed.