CN107215954A - A kind of anaerobism mixed bacterium degradation contains the method for the water body of pollutant nitrobenzene - Google Patents
A kind of anaerobism mixed bacterium degradation contains the method for the water body of pollutant nitrobenzene Download PDFInfo
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- CN107215954A CN107215954A CN201710218007.4A CN201710218007A CN107215954A CN 107215954 A CN107215954 A CN 107215954A CN 201710218007 A CN201710218007 A CN 201710218007A CN 107215954 A CN107215954 A CN 107215954A
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/28—Anaerobic digestion processes
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
- C02F2101/34—Organic compounds containing oxygen
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
- C02F2101/38—Organic compounds containing nitrogen
Abstract
Contain the method for the water body of pollutant nitrobenzene the invention provides a kind of anaerobism mixed bacterium degradation, comprise the following steps：The water body of catalyst, anaerobic sludge, electron donor and nitrobenzene-containing is mixed under anaerobic, reaction solution is obtained；By reaction solution oscillating reactions under anaerobic, the water body after being handled；The catalyst is charcoal, and the granularity of charcoal is 80~120 mesh；The anaerobic sludge includes Pseudomonas aeruginosa, conch Pseudomonas, Desulfovibrio and actinomyces.The method that the present invention is provided, as catalyst, provides mixed bacterium, and can promote the degraded of nitrobenzene in water body under anaerobic using charcoal with anaerobic sludge；This method degrading nitrobenzene speed is very fast, and does not result in secondary pollution.Charcoal cost is low, and is easily made；The technological operation is simple and easy to apply, is easy to promote the use of in practice.
Description
Technical field
Contain pollutant nitro the present invention relates to technical field for the treatment of of organic waste, more particularly to a kind of anaerobism mixed bacterium degradation
The method of the water body of benzene.
Background technology
Nitrobenzene is a kind of important industrial chemicals, is used to production medicine, dyestuff, plastics, explosive and insecticide.But
Its Heavy environmental pollution brought gradually causes everybody attention, and nitrobenzene is considered as important pollutant by many countries.Mesh
Before have many physics and chemistry method can remove nitrobenzene (absorption and advanced oxidation etc.), but these processing means prices
It is expensive or there may be the larger intermediate product of more toxicity.Biochemical method nitrobenzene can generate the relatively low product of toxicity
Aniline, it is clear that bioanalysis is a kind of cheap and effective sewage water treatment method.
At present, primary biological method uses anaerobic reduction degrading nitrobenzene, but its reaction rate is relatively slow, it is necessary to add
Biocatalyst accelerates the process.Recent researches find carbon nanomaterial, such as graphene and CNT, can urge
Change the anaerobic reduction of nitrobenzene.But these materials are the shortcomings of have expensive and bio-toxicity, and its degradation rate is relatively low.
The content of the invention
In view of this, the water body of pollutant nitrobenzene is contained it is an object of the invention to provide a kind of anaerobism mixed bacterium degradation
Method, this method degrading nitrobenzene speed is very fast, and does not result in secondary pollution.
Contain the method for the water body of pollutant nitrobenzene, including following step the invention provides a kind of anaerobism mixed bacterium degradation
Suddenly：
The water body of catalyst, anaerobic sludge, electron donor and nitrobenzene-containing is mixed under anaerobic, reacted
Liquid；
By reaction solution oscillating reactions under anaerobic, the water body after being handled；
The catalyst is charcoal, and the granularity of charcoal is 80~120 mesh；
The anaerobic sludge includes Pseudomonas aeruginosa, conch Pseudomonas, Desulfovibrio and actinomyces.
Preferably, the charcoal is made by following methods：
Wood chip is warming up to 300~800 DEG C of calcinings in nitrogen atmosphere with 4~6 DEG C/min speed, constant temperature 2~4 is small
When, then cooling to 15~35 DEG C, grinding obtains the charcoal that granularity is 80~120 mesh.
Preferably, the volume ratio of the water body of the quality of the anaerobic sludge, the quality of catalyst and nitrobenzene-containing is (0.5
~10) g:(0.5~5) g:1L.
Preferably, the electron donor in glucose, hydrogen, formates, acetate, propionate and butyrate one
Plant or a variety of.
Preferably, the pH value of the reaction solution is 5~10.
Preferably, the temperature of the oscillating reactions is 20~50 DEG C.
Preferably, the rotating speed of the oscillating reactions is 50~500rpm.
Preferably, the concentration of nitrobenzene is 0.25~5mmol/L in the water body of the nitrobenzene-containing.
Preferably, the anaerobic sludge is made by following methods：
Sludge is tamed to no less than 8 cycles in 1~3mmol/L nitrobenzene solution, each cycle is 3~7 days, is obtained
To anaerobic sludge；
The anaerobic sludge includes Pseudomonas aeruginosa, conch Pseudomonas, Desulfovibrio and actinomyces.
Preferably, the porosity of the charcoal is 5.0 × 10-2~5.5 × 10-2cm3g-1；The ratio table of the charcoal
Area is 75~80m2g-1。
Contain the method for the water body of pollutant nitrobenzene, including following step the invention provides a kind of anaerobism mixed bacterium degradation
Suddenly：The water body of catalyst, anaerobic sludge, electron donor and nitrobenzene-containing is mixed under anaerobic, reaction solution is obtained；By institute
State reaction solution oscillating reactions under anaerobic, the water body after being handled；The catalyst is charcoal, the granularity of charcoal
For 80~120 mesh；The anaerobic sludge includes Pseudomonas aeruginosa, conch Pseudomonas, Desulfovibrio and actinomyces.The present invention is provided
Method using charcoal as catalyst, provide mixed bacterium with anaerobic sludge, and nitre in water body can be promoted under anaerobic
The degraded of base benzene；This method degrading nitrobenzene speed is very fast, and does not result in secondary pollution.In addition, the catalysis that this method is used
Agent is charcoal, and its cost is low, and is easily made；The technological operation is simple and easy to apply, is easy to promote the use of in practice.Experimental result
Show：Anaerobic sludge amount is 0.5g, and charcoal is under the conditions of 5g/L, the degradation rate of nitrobenzene is 1~16.25 × 10-2·h-1。
Brief description of the drawings
Fig. 1 is the scanning electron microscope (SEM) photograph of charcoal prepared by the embodiment of the present invention 1；
Fig. 2 is the O1s spectrograms in the XPS spectrum of charcoal prepared by the embodiment of the present invention 1；
Fig. 3 changes over time curve map for material in 3 reactors in the embodiment of the present invention 1.
Embodiment
Contain the method for the water body of pollutant nitrobenzene, including following step the invention provides a kind of anaerobism mixed bacterium degradation
Suddenly：
The water body of catalyst, anaerobic sludge, electron donor and nitrobenzene-containing is mixed under anaerobic, reacted
Liquid；
By reaction solution oscillating reactions under anaerobic, the water body after being handled；
The catalyst is charcoal, and the granularity of charcoal is 80~120 mesh；
The anaerobic sludge includes Pseudomonas aeruginosa, conch Pseudomonas, Desulfovibrio and actinomyces.
The method that the present invention is provided, as catalyst, mixed bacterium is provided with anaerobic sludge using charcoal, and in anaerobic condition
The degraded of nitrobenzene in water body can be promoted down；This method degrading nitrobenzene speed is very fast, and does not result in secondary pollution.Separately
Outside, the catalyst that this method is used is charcoal, and its cost is low, and is easily made；The technological operation is simple and easy to apply, is easy in reality
In promote the use of.
The present invention mixes the water body of catalyst, anaerobic sludge, electron donor and nitrobenzene-containing under anaerobic, obtains
Reaction solution.In the present invention, the catalyst is charcoal；The granularity of the charcoal is 80~120 mesh.The charcoal
Porosity is preferably 5.0 × 10-2~5.510-2cm3g-1；In a particular embodiment of the present invention, the porosity of the charcoal
For 5.113 × 10-2cm3g-1；The specific surface area of the charcoal is preferably 75~80m2g-1；In the specific embodiment of the present invention
In, the specific surface area of the charcoal is 76.9m2g-1。
The charcoal is preferably made by following methods：
Wood chip is warming up to 300~800 DEG C of calcinings in nitrogen atmosphere with 4~6 DEG C/min speed, constant temperature 2~4 is small
When, then cooling to 15~35 DEG C, grinding obtains the charcoal that granularity is 80~120 mesh.
In the present invention, the temperature of the calcining is preferably 350~500 DEG C, more preferably 400 DEG C.The constant temperature when
Between be preferably 3h.
In the present invention, the anaerobic sludge includes Pseudomonas aeruginosa, conch Pseudomonas, Desulfovibrio and actinomyces.Institute
Anaerobic sludge is stated preferably to be made by following methods：
Sludge is tamed to no less than 8 cycles in 1~3mmol/L nitrobenzene solution, each cycle is 3~7 days, is obtained
To anaerobic sludge.
Glucose is preferably added to during domestication as electron donor.The concentration of nitrobenzene is excellent in the nitrobenzene solution
Elect 1.5mmol/L as.The organic loading of the anaerobic sludge is preferably 0.2~0.4kg CODm-3·d-1, more preferably
0.32kg COD·m-3·d-1.The cycle of domestication is preferably 3~7 days, more preferably 5 days.
In the present invention, the electron donor is preferably selected from glucose, hydrogen, formates, acetate, propionate and butyric acid
One or more in salt.In a particular embodiment of the present invention, the formates is preferably sodium formate；The acetate is preferred
For sodium acetate；The propionate is preferably sodium propionate；The butyrate is preferably sodium butyrate.The present invention is to the electron donor
Source, such as can be commercially available using its using above-mentioned electron donor well known to those skilled in the art without special limitation
Commodity.Present invention preferably employs glucose as electron donor, in the reaction solution concentration of glucose be preferably 0.28~
13.88mmol/L, more preferably 1.39~11.1mmol/L, are further preferably 2.78~8.33mmol/L, are most preferably
5.55mmol/L。
In the present invention, the volume ratio of the water body of the quality of the anaerobic sludge, the quality of catalyst and nitrobenzene-containing is excellent
Elect (0.5~10) g as:(0.5~5) g:1L, more preferably (2~8) g：(0.5~1.5) g：1L；Most preferably 5g:1g:1L.
In the present invention, the concentration of nitrobenzene is preferably 0.25~5mmol/L in the water body of the nitrobenzene-containing, more preferably
For 0.5~3.0mmol/L, in a particular embodiment of the present invention, the concentration of nitrobenzene is in the water body of the nitrobenzene-containing
1.0mmol/L.Preferably also include sodium ion, ammonium ion in the water body of the nitrobenzene-containing.
Reacting liquid pH value is preferably adjusted to 5~10, more preferably 7~10 by the present invention；In the specific embodiment of the present invention
In, the pH value of the reaction solution is 7.2.
Catalyst, anaerobic sludge and electron donor are preferably added in the water body of nitrobenzene-containing by the present invention.The present invention is excellent
Using the oxygen in nitrogen removing reaction system, there is provided anaerobic condition for choosing；More preferably using 99.9% high pure nitrogen by oxygen
Remove.
Obtain after reaction solution, the present invention is by reaction solution oscillating reactions under anaerobic, the water body after being handled.
In the present invention, oscillating reactions is carried out preferably under conditions of constant temperature；The temperature of the oscillating reactions is preferably 20~50 DEG C, more
Preferably 35~50 DEG C.The rotating speed of oscillating reactions is preferably 50~500rpm, more preferably 100~400rpm, is further preferably 100
~300rpm；In a particular embodiment of the present invention, the rotating speed of institute's oscillating reactions is 150rpm.
Reaction preferably periodically takes the water body measurement nitro phenenyl concentration after a small amount of processing after starting, until reaction stops.This hair
The bright anaerobic reduction for being used for simulating nitrobenzene using pseudo- First order dynamic model, formula is as follows：
Ct=C0e-k obs t
Wherein, Ct(mmol/L) when being t m-nitro concentration；C0(mmol/L) be nitrobenzene initial concentration；kobs(h-1) it is pseudo- First order kinetic constant；T (h) is the reaction time.
Contain the method for the water body of pollutant nitrobenzene, including following step the invention provides a kind of anaerobism mixed bacterium degradation
Suddenly：The water body of catalyst, anaerobic sludge, electron donor and nitrobenzene-containing is mixed under anaerobic, reaction solution is obtained；By institute
State reaction solution oscillating reactions under anaerobic, the water body after being handled；The catalyst is charcoal, the granularity of charcoal
For 80~120 mesh；The anaerobic sludge includes Pseudomonas aeruginosa, conch Pseudomonas, Desulfovibrio and actinomyces.The present invention is provided
Method using charcoal as catalyst, provide mixed bacterium with anaerobic sludge, and nitre in water body can be promoted under anaerobic
The degraded of base benzene；This method degrading nitrobenzene speed is very fast, and does not result in secondary pollution.In addition, the catalysis that this method is used
Agent is charcoal, and its cost is low, and is easily made；The technological operation is simple and easy to apply, is easy to promote the use of in practice.Experimental result
Show：Anaerobic sludge amount is 0.5g, and charcoal is under the conditions of 5g/L, the degradation rate of nitrobenzene is 1~16.25 × 10-2·h-1。
In order to further illustrate the present invention, a kind of anaerobism mixed bacterium degradation provided with reference to embodiment the present invention contains
The method of the water body of pollutant nitrobenzene is described in detail, but they can not be interpreted as to the limit to the scope of the present invention
It is fixed.
Embodiment 1
Pine tree wood chip is warming up to 400 DEG C in nitrogen atmosphere with 5 DEG C/min of speed, constant temperature 3 hours, then drop naturally
Temperature arrives normal temperature (whole pyrolytic process is carried out in protective atmosphere), grinds and charcoal is made by 100 mesh sieves.
The present invention is scanned electronic microscope photos to obtained charcoal, and it is life prepared by the embodiment of the present invention 1 to see Fig. 1, Fig. 1
The scanning electron microscope (SEM) photograph of thing charcoal.The configuration of surface of the material as seen from Figure 1.
The present invention carries out XPS analysis to charcoal, the results are shown in Table 2, table 2 is charcoal prepared by the embodiment of the present invention 1
O1s spectrograms in XPS spectrum, as seen from Figure 2：There are different oxygen-containing functional groups in the biological carbon materials surface.
The sludge source exists the sludge before formal experiment in the anaerobism upflow sludge bed reactor of processing waste water
No less than 8 cycles (about 5 days each cycles) of domestication in 1.5mmol/L nitrobenzene solution, with glucose as electron donor,
Organic loading is 0.32kg COD m-3·d-1；Anaerobic sludge includes Pseudomonas aeruginosa, conch Pseudomonas, Desulfovibrio and unwrapping wire
Bacterium.Take the desired amount of anaerobic sludge to be centrifuged off supernatant during experiment, then centrifugation is cleaned twice with phosphate buffer salt；
3 220mL reactors are taken, 100mL synthetic media solution is added, are denoted as respectively as No. 1 reactor, No. 2 anti-
No. 3 reactors of device are answered, wherein adding 0.1g catalyst charcoal obtained above in No. 1 reactor, anaerobic sludge are not added with, as
Above-mentioned 0.5g anaerobic sludges are added in 1, No. 2 reactors of control group, catalyst charcoal is not added with, as a control group 2.No. 3 reactions
Device adds anaerobic sludge 0.5g and catalyst charcoal 0.1g.3 reactor sealings are placed in isothermal vibration case, in 35 DEG C of bars
With 150 revs/min of rotating speed concussion reactions under part, periodically sampling detection nitrobenzene degradation situation.The response situation of 3 reactors is shown in
Shown in Fig. 3, Fig. 3 changes over time curve map for material in 3 reactors in the embodiment of the present invention 1；Wherein,It is anti-for No. 1
Answer in device nitrobenzene degrade over time curve andIt is aniline in No. 1 reactor with time formation curve,It is anti-for No. 2
Nitrobenzene in device is answered to degrade over time curve,It is aniline in No. 2 reactors with time formation curve,For No. 3 reactions
In device nitrobenzene degrade over time curve andIt is material in No. 3 reactors with time formation curve.
From figure 3, it can be seen that add charcoal but in the reactor 1 without anaerobic sludge, nitrobenzene after 140 hours
Degrade 24%, no aniline generation.Plus in sludge but the reactor 2 without catalyst, there is 40% nitrobenzene to drop after 30 hours
Solution is with the generation of 0.3mmol/L aniline.And add in the reactor 3 of anaerobic sludge and catalyst, there is 90% nitro after 30 hours
Benzene degraded is simultaneously generated with 0.8mmol/L aniline.The degradation rate of actual conditions and nitrobenzene is shown in Table 1, and table 1 is under different condition
The degradation rate of nitrobenzene：
The degradation rate of nitrobenzene under the different condition of table 1
As can be seen from Table 1：Charcoal can accelerate anaerobism as catalyst and mix thalline system degrading nitrobenzene.
Embodiment 2
14 reactors are taken, numbering is 1~14 respectively, according to table 2 toward adding materials in 14 reactors, nitro phenenyl concentration
For 1mmol/L, anaerobic sludge amount is 0.5g；Anaerobic sludge includes Pseudomonas aeruginosa, conch Pseudomonas, Desulfovibrio and unwrapping wire
Bacterium.Electron donor is respectively：Without (control), glucose, formates, acetate, propionate, butyrate and hydrogen.According to offer
Identical COD determines the content of every kind of material.The reduction conversion of nitrobenzene under the conditions of different electron donors is studied, different electronics are supplied
The degradation rate of nitrobenzene is shown in Table 2 under concrete conditions in the establishment of a specific crime：
The degradation rate of nitrobenzene under the conditions of the different electron donors of table 2
Table 2 shows in the presence of different electron donors that charcoal can accelerate anaerobism as catalyst and mix the degraded of thalline system
Nitrobenzene.When glucose is as electron donor simultaneously, nitrobenzene degradation speed is most fast.
Embodiment 3
14 reactors are taken, numbering is 1~14 respectively, nitro phenenyl concentration is 1mmol/L, anaerobic sludge amount is 0.5g；Detest
Oxygen sludge includes Pseudomonas aeruginosa, conch Pseudomonas, Desulfovibrio and actinomyces.The degradation rate of actual conditions and nitrobenzene is shown in
Table 3：
The degradation rate of nitrobenzene under the conditions of the different electron donors of table 3
Result shows that the concentration for increasing electron donor can accelerate the anaerobic degradation of nitrobenzene in table 3.
Embodiment 4
10 reactors are taken, numbering is 1~10 respectively, anaerobic sludge amount is 0.5g；Anaerobic sludge include Pseudomonas aeruginosa,
Conch Pseudomonas, Desulfovibrio and actinomyces.The degradation rate of actual conditions and nitrobenzene is shown in Table 4：
The degradation rate of nitrobenzene under the conditions of the different nitro phenenyl concentrations of table 4
Table 4 can be seen that：The higher meeting p-nitrophenyl anaerobic degradation of nitro phenenyl concentration causes to suppress, and adding charcoal can be with one
Determine to alleviate this suppression in degree.
Embodiment 5
4 reactors are taken, numbering is 1~4 respectively, anaerobic sludge amount is 0.5g；Anaerobic sludge includes Pseudomonas aeruginosa, sea
Spirillum, Desulfovibrio and actinomyces, nitro phenenyl concentration are 1mmol/L.The degradation rate of actual conditions and nitrobenzene is shown in Table
5：
The degradation rate of nitrobenzene under the conditions of the different biological carbon contents of table 5
Table 5 shows that the increase of biological carbon content can promote the anaerobic degradation of nitrobenzene to a certain extent.
Embodiment 6
6 reactors are taken, numbering is 1~6 respectively, anaerobic sludge amount is 0.5g, and nitro phenenyl concentration is 1mmol/L.Specifically
The degradation rate of condition and nitrobenzene is shown in Table 6：
The degradation rate of nitrobenzene under the condition of different temperatures of table 6
Table 6 shows：Charcoal anaerobic degradation effect is more preferable under hot conditions.
Embodiment 7
Take 10 reactors, respectively numbering be 1~10, anaerobic sludge amount be 0.5g, anaerobic sludge include Pseudomonas aeruginosa,
Conch Pseudomonas, Desulfovibrio and actinomyces, nitro phenenyl concentration are 1mmol/L.The degradation rate of actual conditions and nitrobenzene is shown in
Table 7：
The degradation rate of nitrobenzene under the ammonium ion of table 7 or sodium ion existence condition
Table 7 show a large amount of accumulation of sodium ion and ammonium ion all can the anaerobic degradation of p-nitrophenyl produce suppression.But it is raw
Thing charcoal can alleviate the suppression that both ion bands come as biocatalyst.
As seen from the above embodiment, the water body of pollutant nitrobenzene is contained the invention provides a kind of anaerobism mixed bacterium degradation
Method, comprises the following steps：The water body of catalyst, anaerobic sludge, electron donor and nitrobenzene-containing is mixed under anaerobic,
Obtain reaction solution；By reaction solution oscillating reactions under anaerobic, the water body after being handled；The catalyst is biology
Charcoal, the granularity of charcoal is 80~120 mesh；The anaerobic sludge includes Pseudomonas aeruginosa, conch Pseudomonas, Desulfovibrio and put
Line bacterium.The method that the present invention is provided, as catalyst, provides mixed bacterium, and under anaerobic can using charcoal with anaerobic sludge
Enough promote the degraded of nitrobenzene in water body；This method degrading nitrobenzene speed is very fast, and does not result in secondary pollution.In addition, should
The catalyst that method is used is charcoal, and its cost is low, and is easily made；The technological operation is simple and easy to apply, is easy to push away in practice
Extensively use.Test result indicates that：Anaerobic sludge amount is 0.5g, under the conditions of charcoal is 5g/L, the degradation rate of nitrobenzene for 1~
16.25×10-2h-1。
Described above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, under the premise without departing from the principles of the invention, some improvements and modifications can also be made, these improvements and modifications also should
It is considered as protection scope of the present invention.
Claims (10)
1. a kind of anaerobism mixed bacterium degradation contains the method for the water body of pollutant nitrobenzene, comprise the following steps：
The water body of catalyst, anaerobic sludge, electron donor and nitrobenzene-containing is mixed under anaerobic, reaction solution is obtained；
By reaction solution oscillating reactions under anaerobic, the water body after being handled；
The catalyst is charcoal, and the granularity of charcoal is 80~120 mesh；
The anaerobic sludge includes Pseudomonas aeruginosa, conch Pseudomonas, Desulfovibrio and actinomyces.
2. according to the method described in claim 1, it is characterised in that the charcoal is made by following methods：
Wood chip is warming up to 300~800 DEG C of calcinings in nitrogen atmosphere with 4~6 DEG C/min speed, constant temperature 2~4 hours, then
15~35 DEG C are cooled to, grinding obtains the charcoal that granularity is 80~120 mesh.
3. according to the method described in claim 1, it is characterised in that the quality of the anaerobic sludge, the quality of catalyst are with containing
The volume ratio of the water body of nitrobenzene is (0.5~10) g:(0.5~5) g:1L.
4. according to the method described in claim 1, it is characterised in that the electron donor be selected from glucose, hydrogen, formates,
One or more in acetate, propionate and butyrate.
5. according to the method described in claim 1, it is characterised in that the pH value of the reaction solution is 5~10.
6. according to the method described in claim 1, it is characterised in that the temperature of the oscillating reactions is 20~50 DEG C.
7. method according to claim 6, it is characterised in that the rotating speed of the oscillating reactions is 50~500rpm.
8. according to the method described in claim 1, it is characterised in that the concentration of nitrobenzene is in the water body of the nitrobenzene-containing
0.25~5mmol/L.
9. according to the method described in claim 1, it is characterised in that the anaerobic sludge is made by following methods：
Sludge is tamed to no less than 8 cycles in 1~3mmol/L nitrobenzene solution, each cycle is 3~7 days, is detested
Oxygen sludge；
The anaerobic sludge includes Pseudomonas aeruginosa, conch Pseudomonas, Desulfovibrio and actinomyces.
10. according to the method described in claim 1, it is characterised in that the porosity of the charcoal is 5.0 × 10-2~5.5 ×
10-2cm3g-1；The specific surface area of the charcoal is 75~80m2g-1。
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