CN107213972A - The mannosan and its anti-tumor virus applications prepared based on nanometer technology - Google Patents
The mannosan and its anti-tumor virus applications prepared based on nanometer technology Download PDFInfo
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- CN107213972A CN107213972A CN201710346353.0A CN201710346353A CN107213972A CN 107213972 A CN107213972 A CN 107213972A CN 201710346353 A CN201710346353 A CN 201710346353A CN 107213972 A CN107213972 A CN 107213972A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B02—CRUSHING, PULVERISING, OR DISINTEGRATING; PREPARATORY TREATMENT OF GRAIN FOR MILLING
- B02C—CRUSHING, PULVERISING, OR DISINTEGRATING IN GENERAL; MILLING GRAIN
- B02C21/00—Disintegrating plant with or without drying of the material
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B02—CRUSHING, PULVERISING, OR DISINTEGRATING; PREPARATORY TREATMENT OF GRAIN FOR MILLING
- B02C—CRUSHING, PULVERISING, OR DISINTEGRATING IN GENERAL; MILLING GRAIN
- B02C19/00—Other disintegrating devices or methods
- B02C19/06—Jet mills
- B02C19/061—Jet mills of the cylindrical type
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B02—CRUSHING, PULVERISING, OR DISINTEGRATING; PREPARATORY TREATMENT OF GRAIN FOR MILLING
- B02C—CRUSHING, PULVERISING, OR DISINTEGRATING IN GENERAL; MILLING GRAIN
- B02C19/00—Other disintegrating devices or methods
- B02C19/16—Mills provided with vibrators
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B02—CRUSHING, PULVERISING, OR DISINTEGRATING; PREPARATORY TREATMENT OF GRAIN FOR MILLING
- B02C—CRUSHING, PULVERISING, OR DISINTEGRATING IN GENERAL; MILLING GRAIN
- B02C19/00—Other disintegrating devices or methods
- B02C19/22—Crushing mills with screw-shaped crushing means
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/006—Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence; Gellans; Succinoglycans; Arabinogalactans; Tragacanth or gum tragacanth or traganth from Astragalus; Gum Karaya from Sterculia urens; Gum Ghatti from Anogeissus latifolia; Derivatives thereof
- C08B37/0087—Glucomannans or galactomannans; Tara or tara gum, i.e. D-mannose and D-galactose units, e.g. from Cesalpinia spinosa; Tamarind gum, i.e. D-galactose, D-glucose and D-xylose units, e.g. from Tamarindus indica; Gum Arabic, i.e. L-arabinose, L-rhamnose, D-galactose and D-glucuronic acid units, e.g. from Acacia Senegal or Acacia Seyal; Derivatives thereof
- C08B37/009—Konjac gum or konjac mannan, i.e. beta-D-glucose and beta-D-mannose units linked by 1,4 bonds, e.g. from Amorphophallus species; Derivatives thereof
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Abstract
Preparation facilities, including thickness part and the part that becomes more meticulous are refined the invention discloses a kind of nanometer of mannosan, thickness flower part carries out refinement work by motor driving；Becoming more meticulous partly includes airslide disintegrating mill, and is provided with ultrasonic wave portion progress auxiliary refinement work；The present apparatus is by regulation motor rotating speed, the frequency of ultrasonic wave and the time of refinement, so as to produce the mannosan that particle diameter is 40,000 8 ten thousand Da in 80 150nm and range of molecular weight distributions；Corresponding product is made or applied to being taken in antitumor product in these nanometer of mannosan, there is obvious inhibitory action to tumour；These nanometer of mannosan is subjected to Sulfation derivatization treatment, corresponding product is made or applied in anti-virus product, with good broad-spectrum antiviral effect.
Description
Technical field
The present invention relates to a kind of nanometer refinement technology, further relate to using the mannosan of technology preparation and its antitumor
With it is antiviral in application.
Background technology
Nanometer (English：Nanometre) it is long measure, International System of Units symbol is nm.Original claims millimicron, is exactly 10-9
Rice (1/1000000000th meter), i.e., 10-6Millimeter (1/1000000th millimeters).As centimetre, decimeter with meter as, be length
Linear module.Equivalent to 4 times atom sizes, the length than single bacterium is also small.International common name is nanometer, letter
Write nm.
It is present scientific investigations showed that, the particle diameter of particle can influence the distribution of medicine in vivo, and the particulate that 5 μm of particle diameter ＜ can
Blood circulation can be entered with the particulate by lung, particle diameter ＜ 300nm, particle diameter ＜ 100nm particulate can enter marrow, nanometer
Medicine obtains oral, nasal-cavity administration, the bioavilability of Transdermal absorption medicine more easily by stomach, intestinal mucosa and nasal membrane
To raising.The nanosizing of particle can show many excellent performances, be embodied in quantum size effect, small size effect
Should, macro quanta tunnel effect, skin effect etc..
The preparation method of current nano-particle is generally divided into two major classes：Physical method and chemical method.Physical method is also known as
For comminuting method, it is cake mass to be crushed to solid material from large to small, i.e., nano-powder particle is made；Chemical method is also known as
Method for constructing, it is to pass through two stage nano materials of nucleation and growth by lower limit atom, ion, molecule.Using chemistry as base
The nano-powder manufacture method of plinth can obtain several nanometers of powder.But manufacturing cost is sometimes at a relatively high, and it is difficult amplification, particle diameter
Distribution is also more uneven.At present, nanometer pulverization equipment mainly has multi-D swing high energy nanon ball-mill, multilayer classifying nano
Ball mill, high speed nano grinder, high speed shear ultrafine crusher, airslide disintegrating mill, ultrasonic wave nano grinder etc..
The main component of konjaku powder is SKGM, also known as konjaku glucomannan (KGM), is viscosity in known plants glue
Maximum natural macromolecule amylose, mean molecule quantity is 200,000-200 ten thousand Da (dalton), and profile is white or cream is to light brown
Yellow powder, be by molecular proportion be 1：1.6 glucose and mannose residue is polymerized by β-(Isosorbide-5-Nitrae)-glycosidic bond, at certain
Exist on a little saccharide residue C-3 on the side chain of β (1,3) glycosidic bond composition, main chain has 1 side chain, every branch per 32-80 saccharide residue
Chain has on several to tens saccharide residues, main chain every about having an acetyl group combined with ester bond on 19 saccharide residues.Utilize
The KGM weight average molecular weight that light scattering method is measured is 1.12 × 106Or 2.619 × 105, and the viscosity average molecular weigh for measuring KGM is 8.09
×l05.KGM solids have between regular fibrous strands pattern, KGM and there are a large amount of hydrogen bonds, do not form stable crystallization, and
It is to exist with amorphous state；KGM in presence of water, by substantial amounts of hydrogen bond is formed in molecule interchain to maintain micelle in solution
State, and micelle size in nanoscale.
Konjak glucomannan has an a variety of excellent characteristics, such as gelation, edibility, film forming, thus food,
Each production field such as medicine, chemical industry has extensive purposes.But KGM has the characteristics such as the low, poor fluidity of solubility, it is applied
It is subject to certain restrictions, is the further performance for improving KGM, expands its application, generally pass through Physical, chemical method and life
The means such as thing method are degraded to it.
The fracture of high molecular polymer can be generally divided on microcosmic it is intermolecular slip, Van der Waals force or hydrogen bond destruction and
Chemical bond destroys three types.The destruction of first two type can't cause the degraded of molecule, and the destruction of only chemical bond just may be used
The degraded of molecule can be caused.Polymer is plasticated in polymerization, melting extrusion, and Polymer Solution is made by strong agitation or ultrasonic wave
Used time, macromolecular chain may be all set to be broken and degrade.The mechanical force and chemical signs of degradation of konjaku glucomannan is for actual production
With important inspiration meaning.
Particle be small solid, liquid or the gas being under cutting state or have vital microorganism,
Bacterium, virus etc..In most cases, the word of particle one refers to solid particle, and liquid particles and gas particles then correspondingly turn into
Drop and bubble.The particle swarm being made up of many particles turns into particle system.Powder is then solid particle under rarefaction
Accumulation.Particle swarm or particle system are made up of many particles.If all particles of composition particle swarm are respectively provided with identical or near
Like identical granularity, then the particle swarm is called monodispersed.When particle swarm is made up of particle not of uniform size, then as many points
Scattered.
Current nanometer pulverization technology is varied, but the nano-scale particle after crushing can not accurately control its molecule
After amount is, it is necessary to crush, then screening is measured, so as to obtain the product of needs.Substantial amounts of work has just been lost in this.
Tumour is the chronic disease for seriously endangering health, and its maximum harm is exactly that cell mutation causes organ failure to make
People is dead.Tumour is difficult directly to be found by people in early stage, and when body-feeling is to discomfort, and often infantile tumour has been also
Through the malignant tumour for becoming the later stage.
The viral infection of the mankind is very universal, and existence and quality of life of the virus infection to the mankind have huge shadow
Ring.It is some virus infection even it is relevant with the generation of tumour, such as primary carcinoma of liver it is relevant with hepatitis B virus infection, cervix cancer and
Human nipple warty virus (HPV) is relevant etc..The mankind are finding effective antiviral methods diligently always.
The content of the invention
There is provided the nanometer preparation facilities and method of a kind of mannosan, energy for deficiency of the prior art by the present invention
Enough particle diameters by controlling product after refining, so as to accurately control the molecular weight of product after dispersion and fining, obtain molecular weight
The mannosan concentrated is distributed, the technical method is more convenient, environmental protection, more commercial competitiveness；And referred to using device production
Determine the nanoscale SKGM in molecular weight ranges, and after being eaten, tumour growth can be suppressed；It is again that nanoscale konjaku is more
After sugar sulfate derivatization treatment, with good antivirus action.
In order to solve the above-mentioned technical problem, the present invention is addressed by following technical proposals：A kind of mannosan is received
Stirring for spiral above and below being provided with stirring rod, the stirring rod is provided with rice preparation plant, including pulverization cylinder, the pulverization cylinder
Leaf is mixed, the pulverization cylinder bottom is provided with motor, and the output shaft of the motor is connected with the stirring rod, the pulverization cylinder bottom
Be provided with feed pipe, be provided with discharge belt at the top of the pulverization cylinder, the discharge belt close to the paddle most
Cooling cylinder is provided with outside upper end, the pulverization cylinder, the cooling cylinder bottom is provided with water inlet pipe and is connected with out on upper side wall
Water pipe, the other end of the discharge belt is arranged above aggregate bin, and pump is connected with below the aggregate bin, and the pump is set
There is discharging portion, the discharging portion is provided with the first discharge nozzle and the second discharge nozzle, and first discharge nozzle is connected with air-flow crushing
Machine, the air-flow crushing pusher side wall is provided with the first air jet pipe and the second air jet pipe, and the is provided with the top of the airslide disintegrating mill
Three air jet pipes, the axis of first air jet pipe, the second air jet pipe and the 3rd air jet pipe intersects at a point, second discharging
Pipe is connected with the first high-pressure pump and the second high-pressure pump, and the output end of first high-pressure pump connects first air jet pipe, described
The output end of second high-pressure pump connects second air jet pipe, and the 3rd air jet pipe is connected with the 3rd high-pressure pump, the air-flow
Crush motor spindle and be provided with discharge nozzle, the discharge nozzle is connected with material transfer portion, the material transfer portion connection described first
High-pressure pump, the second high-pressure pump and the 3rd high-pressure pump, the material transfer portion are also associated with setting in finished bin, the airslide disintegrating mill
It is placed in baffle portion, the baffle portion and is provided with drainage inclined-plane, is set between drainage inclined-plane and the airslide disintegrating mill top
The drainage cambered surface of arc is equipped with, the drainage cambered surface end is connected with the 3rd air jet pipe, is provided with and grinds in the pulverization cylinder
Annular is provided with two circle ultrasonic wave portions on abrading-ball, the airslide disintegrating mill outer wall, often encloses ultrasonic wave portion and is provided with odd number ultrasound
Wave producer.
A kind of method that nanometer mannosan is produced using above-mentioned nanometer preparation facilities, including step one are more by konjaku
Sugared material is pulverizing processing 30-150 minutes in pulverization cylinder, and the velocity of rotation of stirring rod is 1000-5000 revs/min；Step 2, is opened
Dynamic discharge belt, paddle is rotated the material brought up and is delivered in aggregate bin；Step 3, starts pump by aggregate bin
Material is delivered to airslide disintegrating mill by the first discharge nozzle, and is delivered to the first high-pressure pump by the second discharge nozzle；Step 4,
Start the first high-pressure pump, material is sprayed into the material phase exported in airslide disintegrating mill and from the first discharge nozzle by the first air jet pipe
Collision, then stops the first high-pressure pump；Step 5, starts the second high-pressure pump, and material is sprayed into air-flow powder by the second air jet pipe
Collided in broken machine with internal material, then stop the second high-pressure pump；Step 6, starts the 3rd high-pressure pump, material is passed through
3rd air jet pipe is sprayed into airslide disintegrating mill and internal material collides, and then stops the 3rd high-pressure pump；Step 7, repeats to walk
Rapid three to six, repetition time 20-90 minute, while starting supersonic generator, the frequency of ultrasonic wave is 30k-90kHz；Step
Eight, material transfer portion is by the material transportation collected from discharge nozzle to finished bin, and the material in finished bin is a nanometer mannosan.
A kind of nanometer mannosan of use above method production, the particle diameter of the nanometer mannosan is 80-150nm,
Molecular weight distribution is 40,000-8 ten thousand Da.Mannosan in this particle diameter and molecular weight ranges is referred to as the sub- glycan of sweet dew time or received
Meter Gan Lu sub- glycan.
A kind of application of nanometer mannosan of above-mentioned production in antitumor, corresponding product is made by nanometer mannosan
Or applied to being taken in antitumor product, the nanometer mannosan and the ratio of body weight taken are 100-400mg/kg, take and receive
There is obvious inhibitory action to tumour after rice mannosan.
A kind of application of nanometer mannosan of above-mentioned production in anti-virus product, the nanometer mannosan is carried out
Sulphation derivatization treatment, is made corresponding product or applied to being used in anti-virus product so that sulphation sweet dew time is sub-
Glycan is in 200 μ g/ml concentrations above, with good broad-spectrum antiviral effect.
SKGM is crushed to nano level particle by the present invention using nanometer pulverization device, controls the particle diameter of these particles
In the range of 80-150nm, so that polyoses grain of the molecular weight distribution in 40,000-8 ten thousand Da is obtained, can be by these polysaccharide
Grain is named as a nanometer mannosan, then it is mixed into according to certain body weight ratio after eating, can suppress tumour growth, from
And can be applied in antitumor product.After this nanometer of mannosan Sulfation derivatization treatment, with disease-resistant well
Toxic action.
Brief description of the drawings
Fig. 1 is pulverization cylinder structural representation of the present invention.
Fig. 2 is airslide disintegrating mill structural representation of the present invention.
Fig. 3 is Pneumatic crushing machine gaseous flow diagram of the invention.
Embodiment
The present invention is described in further detail with embodiment below in conjunction with the accompanying drawings：As shown in Figure 1 to Figure 3, one
Plant in the nanometer preparation facilities of mannosan, including pulverization cylinder 1, the pulverization cylinder 1 and be provided with stirring rod 13, the stirring rod 13
On be provided with above and below spiral paddle 131, the bottom of pulverization cylinder 1 is provided with motor 11, the output shaft 12 of the motor 11
It is connected with the stirring rod 13, the bottom of pulverization cylinder 1 is provided with feed pipe 14, the top of pulverization cylinder 1 is provided with discharging and passed
Band 15 is sent, the discharge belt 15 is provided with cooling cylinder 2 outside the top of the paddle 131, the pulverization cylinder 1,
The bottom of cooling cylinder 2 is provided with water inlet pipe 21 and outlet pipe 22 is connected with upper side wall, the discharge belt 15 it is another
One end is arranged at the top of aggregate bin 31, and the lower section of aggregate bin 31 is connected with pump 3, and the pump 3 is provided with discharging portion 32, it is described go out
Material portion 32 is provided with the first discharge nozzle 321 and the second discharge nozzle 322, and first discharge nozzle 321 is connected with airslide disintegrating mill 4,
The side wall of airslide disintegrating mill 4 is provided with the first air jet pipe 5 and the second air jet pipe 6, and the top of airslide disintegrating mill 4 is provided with the
Three air jet pipes 42, the axis of first air jet pipe 5, the second air jet pipe 6 and the 3rd air jet pipe 42 intersects at a point, and described
Two discharge nozzles 322 are connected with the first high-pressure pump and the second high-pressure pump, output end connection first spray of first high-pressure pump
Tracheae 5, the output end of second high-pressure pump connects second air jet pipe 6, and it is high that the 3rd air jet pipe 42 is connected with the 3rd
Press pump, the bottom of airslide disintegrating mill 4 is provided with discharge nozzle 41, and the discharge nozzle 41 is connected with material transfer portion, the material
Transhipment department connects first high-pressure pump, the second high-pressure pump and the 3rd high-pressure pump, and the material transfer portion is also associated with finished bin,
It is arranged in the airslide disintegrating mill 4 in baffle portion 45, the baffle portion 45 and is provided with drainage inclined-plane 44, the drainage inclined-plane 44
The drainage cambered surface 43 of arc, the end of drainage cambered surface 43 and the described 3rd are provided between the top of airslide disintegrating mill 4
Air jet pipe 42 be connected, be provided with the pulverization cylinder 1 annular on mill ball, the outer wall of airslide disintegrating mill 4 be provided with two circles surpass
Sound wave portion, often encloses ultrasonic wave portion and is provided with odd number supersonic generator 7.
A kind of method that nanometer mannosan is produced using above-mentioned nanometer preparation facilities, including step one are more by konjaku
Sugared material is pulverizing processing 30-150 minutes in pulverization cylinder 1, and the velocity of rotation of stirring rod is 1000-5000 revs/min；Step 2,
Start discharge belt 15, paddle 131 is rotated to the material brought up and is delivered in aggregate bin 31；Step 3, starting pump 3 will
Material in aggregate bin 31 is delivered to airslide disintegrating mill 4 by the first discharge nozzle 321, and is conveyed by the second discharge nozzle 322
To the first high-pressure pump；Step 4, starts the first high-pressure pump, by material sprayed into by the first air jet pipe 5 in airslide disintegrating mill 4 and from
The material of first discharge nozzle 321 output collides, and then stops the first high-pressure pump；Step 5, starts the second high-pressure pump, by material
Sprayed into by the second air jet pipe 6 in airslide disintegrating mill 4 and internal material collides, then stop the second high-pressure pump；Step 6,
Start the 3rd high-pressure pump, material is sprayed into airslide disintegrating mill 4 by the 3rd air jet pipe 42 and internal material collides, then
Stop the 3rd high-pressure pump；Step 7, repeat step three to six, repetition time 20-90 minute, while start supersonic generator 7,
The frequency of ultrasonic wave is 30k-90kHz；Step 8, material transfer portion by the material transportation collected from discharge nozzle 41 to finished bin,
Material in finished bin is a nanometer mannosan.
A kind of nanometer mannosan of use above method production, the particle diameter of the nanometer mannosan is 80-150nm,
Molecular weight distribution is 40,000-8 ten thousand Da.Mannosan in this particle diameter and molecular weight ranges is referred to as the sub- glycan of sweet dew time or received
Meter Gan Lu sub- glycan.
A kind of application of nanometer mannosan of above-mentioned production in antitumor, the nanometer mannosan is made accordingly
Product or applied to being taken in antitumor product, the nanometer mannosan and the ratio of body weight taken is 100-400mg/kg, clothes
With there is obvious inhibitory action to tumour after nanometer mannosan.
A kind of application of nanometer mannosan of above-mentioned production in antiviral, sulfuric acid is carried out by the nanometer mannosan
Change derivatization treatment, corresponding product is made or applied to being used in anti-virus product so that sulphation sweet dew time Asia glycan
In 200 μ g/ml concentrations above, with good broad-spectrum antiviral effect.
Controlling sulfate polyose, which refers to that the natural and semi-synthetic acidity containing sulfate group, to be warded off, and is polyanion chemical combination
Thing, found that Sulfation glucan has had since suppressing AIDS virus HIV activity from 1987, the saccharoidal antiviral side
The research in face is very active, and it is likely to become the another class after viral reverse transcriptase activity inhibitor, protease inhibitors
HIV-1 inhibitor.The sub- glycan of sulphation sweet dew time is that the sub- glycan of sweet dew time is carried out into sulphation by chlorosulfonic acid-pyridine method to repair
Decorations, sulphation modification can significantly improve the bioactivity of the sub- glycan of sweet dew time, especially antiviral activity.
The product cut size obtained by the nanometer pulverization technology of the present invention, overall particle diameter distribution approximate normal distribution, absolutely
Most of particle diameter concentrates on 80-150nm, then the molecular weight distribution for obtaining product by detection is 40,000-8 ten thousand Da.Mechanical force is forced
KGM meta system is made original macromolecular chain and hydrogen bond fracture obtain relatively low sweet of molecular weight by uniform sequential cutting
Reveal glycan.Mannosan in this particle diameter and molecular weight ranges is referred to as the sub- glycan of sweet dew time or the sub- glycan of nanometer sweet dew time.
The nanometer refinement technology of the present invention is the method for mixing refinement, by the grinding time of control machine, and rotating speed is controlled
The particle diameter of finished product after system is crushed.
Under prior art, the molecular weight of detection polysaccharide is a sufficiently complex cumbersome process, especially to passing through chemistry
Or the mannosan that biological mode is obtained carries out detection molecules amount, even more one very difficult operation.There is provided in this programme
Device, the particle diameter overall distribution of the mannosan product of production is similar to normal distribution.Entered multiple detection contrast to find, this
The particle diameter for the mannosan that device is produced by the above method is in the range of 80-150nm, meanwhile, corresponding molecular weight point
It is distributed between 40,000-8 ten thousand Da.A kind of corresponding relation is presented in the mannosan of present apparatus production, its particle diameter and molecular weight.This is just big
Detection process is simplified greatly, as long as the particle diameter of detection particle, so as to save the substantial amounts of working time.That is, passing through this
The mannosan of device production, as long as by size controlling in the range of 50-80nm, it becomes possible to ensure molecular weight distribution in 40,000-
Between 80000 Da.
SKGM is crushed to nano level particle by the present invention using nanometer pulverization device, by controlling these particles
These polyoses grains, so as to obtain polyoses grain of the molecular weight distribution in the range of anticipation, are named as a nanometer sweet dew by particle diameter
It, is then mixed into after eating, can suppress tumour growth, so as in antitumor production by glycan according to certain body weight ratio
Applied in product.After this nanometer of mannosan sulphation derivatization treatment, with good antiviral effect.
The present invention is 30-150 minutes by adjusting SKGM material pulverizing processing time in pulverization cylinder 1, simultaneously will
The velocity of rotation of stirring rod 13 is set as 1000-5000 revs/min, after preliminary crushing, then is transported to air-flow crushing
The fine crushing of 20-90 minutes when entering behavior in machine 4, while controlling the frequency of ultrasonic wave, its scope is limited to as 30k-
Between 90kHz.SKGM particle of the particle diameter within 80-150nm finally is resulted in, its molecular weight distribution is in 40,000-8 ten thousand
Between Da.
Experiment case study one：Anti-tumor experiment
Specific embodiment is：Select the inoculated tumour mouse of 10 days, aseptic aspiration mouse ascites, with 0.9% chlorination
3 times of amount dilutions of sodium injection, adjustment oncocyte number is 1 × 108/ml, to every mouse armpit subcutaneous vaccination 0.2ml.Random packet, connects
Start within 24 hours administration after kind, control group is the physiological saline of equivalent, 1 time a day, 5 FU 5 fluorouracil group 10mg/kg, using skin
Lower injection, 1 times a week, the sub- glycan of sweet dew time it is low middle high and two groups be respectively 100mg/kg, 200mg/kg, 400mg/kg, use
Gastric infusion, 1 time a day, continuous 7 days, the neck that breaks put to death tumor-bearing mice, peels off tumor mass, claims knurl weight, calculates tumour inhibiting rate.
Inhibitory action result of the test such as table 1 below of the sub- glycan of sweet dew time to small s-180 sarcoma：
| Group | Sample | Dosage (mg/kg) | Average knurl weight ± SD | Tumour inhibiting rate (%) |
| Control group (salt solution) | 10 | 0 | 2.21±0.43 | — |
| 5-FU | 10 | 10 | 0.52±0.35 | 76.5%** |
| The sub- glycan of low dosage sweet dew time | 10 | 100 | 1.81±0.48 | 18.1%* |
| The sub- glycan of middle dosage sweet dew time | 10 | 200 | 1.49±0.26 | 32.6%* |
| The sub- glycan of high dose sweet dew time | 10 | 400 | 0.98±0.05 | 55.7%** |
Compared with control group, P ＜ 0.01；* compared with control group, P ＜ 0.05.
It is verified by experiments：After experiment mice transplanting S-180 sarcomas, second day according to the sub- glycan 100mg/kg of sweet dew time,
200mg/kg, 400mg/kg are administered, continuous 7 days, determination experiment group and the average knurl weight of control group, as a result show the sub- glycan of sweet dew time
There is obvious inhibitory action (P ＜ 0.01) to mouse transplantability S-180 sarcomas in 400mg/kg.
Experiment case study two：Antiviral breeding
Current antiviral activity cell assay in vitro assay method mainly include cytopathic-effect inhibition assay, pH value transformation method,
Decoration method reduces method with plaque, and this experiment reduces method using cytopathic-effect inhibition assay and decoration method with plaque.
(1) the sub- glycan of sulphation sweet dew time is tested to cytopathic effect inhibition
The sub- glycan of sulphation sweet dew time is diluted in acellular poison concentration range, with decoration method and micro- sem observation
Its suppression to cytopathy caused by herpes simplex virus HSV-2, with the increase of concentration, the sub- glycan of sulphation sweet dew time
Suppress cytopathic effect and gradually become strong.Such as table 1 below,
Effect of the sub- glycan of sulphation sweet dew time of table 1 to cytopathy caused by HSV-2
Note：Symbol is represented respectively in table 1：—：Acellular lesion；±：Cytopathy is less than 5%；+：Cytopathy 5%-
25%；++：Cytopathy 25%-50%；+++：Cytopathy 50%-75%；++++：Cytopathy 75%-100%.
(2) the sub- glycan of sulphation sweet dew time is to herpes simplex virus HSV-2 plaque Inhibition tests
Under 200-1600 μ g/ml concentration, the sub- glycan of sulphation sweet dew time has been produced substantially under 200 μ g/ml concentration
Virus plaques formation inhibitory action.The formation of virus, specific experiment result can be completely inhibited in 400 μ g/ml concentrations above
Such as table 2 below：
Effect of the sub- glycan of sulphation sweet dew time of table 2 to herpes simplex virus HSV-2 plaques
Note：Symbol is represented respectively in table 1：—：Acellular lesion；±：Cytopathy is less than 5%；+：Cytopathy 5%-
25%；++：Cytopathy 25%-50%；+++：Cytopathy 50%-75%；++++：Cytopathy 75%-100%.
Protection scope of the present invention includes but is not limited to embodiment of above, and protection scope of the present invention is with claims
It is defined, any replacement being readily apparent that to those skilled in the art that this technology is made, deformation, improvement each fall within the present invention's
Protection domain.
Claims (5)
1. a kind of nanometer preparation facilities of mannosan, it is characterised in that：Including pulverization cylinder（1）, the pulverization cylinder（1）It is interior to set
There is stirring rod（13）, the stirring rod（13）On be provided with above and below spiral paddle（131）, the pulverization cylinder（1）Bottom is set
It is equipped with motor（11）, the motor（11）Output shaft（12）With the stirring rod（13）It is connected, the pulverization cylinder（1）Bottom is set
It is equipped with feed pipe（14）, the pulverization cylinder（1）Top is provided with discharge belt（15）, the discharge belt（15）Close to institute
State paddle（131）The top, the pulverization cylinder（1）It is provided with cooling cylinder outside（2）, the cooling cylinder（2）Bottom is provided with
Water inlet pipe（21）And it is connected with outlet pipe on upper side wall（22）, the discharge belt（15）The other end be arranged at aggregate bin
（31）Top, the aggregate bin（31）Lower section is connected with pump（3）, the pump（3）It is provided with discharging portion（32）, the discharging portion
（32）It is provided with the first discharge nozzle（321）With the second discharge nozzle（322）, first discharge nozzle（321）It is connected with air-flow crushing
Machine（4）, the airslide disintegrating mill（4）Side wall is provided with the first air jet pipe（5）With the second air jet pipe（6）, the airslide disintegrating mill
（4）Top is provided with the 3rd air jet pipe（42）, first air jet pipe（5）, the second air jet pipe（6）With the 3rd air jet pipe（42）'s
Axis intersects at a point, second discharge nozzle（322）It is connected with the first high-pressure pump and the second high-pressure pump, first high pressure
The output end of pump connects first air jet pipe（5）, output end connection second air jet pipe of second high-pressure pump（6）,
3rd air jet pipe（42）It is connected with the 3rd high-pressure pump, the airslide disintegrating mill（4）Bottom is provided with discharge nozzle（41）, it is described
Discharge nozzle（41）Material transfer portion is connected with, the material transfer portion connects first high-pressure pump, the second high-pressure pump and the 3rd
High-pressure pump, the material transfer portion is also associated with finished bin, the airslide disintegrating mill（4）Inside it is arranged at baffle portion（45）, it is described
Baffle portion（45）On be provided with drainage inclined-plane（44）, the drainage inclined-plane（44）With the airslide disintegrating mill（4）Set between top
It is equipped with the drainage cambered surface of arc（43）, the drainage cambered surface（43）End and the 3rd air jet pipe（42）It is connected, it is described to crush
Cylinder（1）Inside it is provided with mill ball, the airslide disintegrating mill（4）Annular is provided with two circle ultrasonic wave portions on outer wall, often encloses ultrasonic wave
Portion is provided with odd number supersonic generator（7）.
2. the method that the nanometer preparation facilities described in a kind of utilization claim 1 produces nanometer mannosan, it is characterised in that：Bag
Step one is included, by SKGM material in pulverization cylinder（1）Interior pulverizing processing 30-150 minutes, stirring rod（13）Velocity of rotation be
1000-5000 revs/min；Step 2, starts discharge belt（15）, by paddle（131）The material brought up is rotated to be delivered to
Aggregate bin（31）It is interior；Step 3, starts pump（3）By aggregate bin（31）Interior material passes through the first discharge nozzle（321）It is delivered to gas
Flow pulverizer（4）, and pass through the second discharge nozzle（322）It is delivered to the first high-pressure pump；Step 4, starts the first high-pressure pump, will
Material passes through the first air jet pipe（5）Spray into airslide disintegrating mill（4）It is interior and from the first discharge nozzle（321）The material of output collides,
Then the first high-pressure pump is stopped；Step 5, starts the second high-pressure pump, material is passed through into the second air jet pipe（6）Spray into air-flow crushing
Machine（4）Interior and internal material collides, and then stops the second high-pressure pump；Step 6, starts the 3rd high-pressure pump, material is passed through
3rd air jet pipe（42）Spray into airslide disintegrating mill（4）Interior and internal material collides, and then stops the 3rd high-pressure pump；Step
Seven, repeat step three to six, repetition time 20-90 minute, while starting supersonic generator（7）, the frequency of ultrasonic wave is
30k-90kHz；Step 8, material transfer portion will be from discharge nozzle（41）The material transportation of collection is to finished bin, the thing in finished bin
Material is a nanometer mannosan.
3. a kind of nanometer mannosan of the method production described in utilization claim 2, it is characterised in that：The nanometer sweet dew gathers
The particle diameter of sugar is 80-150nm, and molecular weight distribution is 40,000-8 ten thousand Da.
4. application of the nanometer mannosan in antitumor described in a kind of claim 3, it is characterised in that：The nanometer is sweet
Corresponding product is made or applied to being taken in antitumor product in dew glycan, and the nanometer mannosan and the ratio of body weight taken are
100-400mg/kg, take has obvious inhibitory action to tumour after nanometer mannosan.
5. application of the nanometer mannosan in antiviral described in a kind of claim 3, it is characterised in that：The nanometer is sweet
Reveal glycan and carry out Sulfation derivatization treatment, corresponding product is made or applied to being used in anti-virus product so that sulphur
The sub- glycan of sweet dew time is acidified in 200 μ g/ml concentrations above, with good broad-spectrum antiviral effect.
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Cited By (2)
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| CN107955826A (en) * | 2017-12-14 | 2018-04-24 | 宁波拜尔玛生物科技有限公司 | A kind of preparation method and applications of the sub- glycan of high-purity sweet dew time |
| CN110270426A (en) * | 2019-06-26 | 2019-09-24 | 厦门一泰消防科技开发有限公司 | Extinguishing chemical and preparation method thereof based on nanometer pulverization technology |
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| CN110270426A (en) * | 2019-06-26 | 2019-09-24 | 厦门一泰消防科技开发有限公司 | Extinguishing chemical and preparation method thereof based on nanometer pulverization technology |
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| CN107213972B (en) | 2019-06-04 |
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