CN107192818A - A kind of particulate colourity clustering method and kit - Google Patents
A kind of particulate colourity clustering method and kit Download PDFInfo
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- CN107192818A CN107192818A CN201710370975.7A CN201710370975A CN107192818A CN 107192818 A CN107192818 A CN 107192818A CN 201710370975 A CN201710370975 A CN 201710370975A CN 107192818 A CN107192818 A CN 107192818A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/585—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with a particulate label, e.g. coloured latex
Abstract
The invention discloses a kind of particulate colourity clustering method and kit.This method includes：Biological targets different from the M kinds in sample to be measured specific binding reaction can occur for S1, structure biological target detection reagent, the biological target detection reagent respectively simultaneously；S2, progress biological detection reaction, form the sub- bioprobe I of color particles biological target separation agent conjugate to be measured；S3, separation biological detection reaction product, the sub- polymer of bioprobe I of the color particles for not occurring association reaction with detection reagent is separated；S4, testing result judge, according to the sub- colourity of color particles and the one-to-one relationship of biological target species foundation, the species and content of the biological target to be measured contained in statistic mixed-state sample.Meanwhile, present invention correspondence provides corresponding kit.The present invention, which is realized, realizes simple sample Multiple detection and the quick Multiple detection of many samples.
Description
Technical field
The invention belongs to field of biomedicine technology, and in particular to a kind of particulate colourity clustering method and reagent
Box.
Background technology
Large biological molecule, including protein, nucleic acid etc., embody or recite life and the information of disease, be that biology is ground
Study carefully with analysis object particularly important in medical diagnosis on disease, detection the presence of which and content, is scientific research and medical diagnosis on disease
Element task；Except biomacromolecule detection, in some of medical diagnosis on disease and biological study occasion, it is also desirable to virus,
Cell is detected that these biomolecule, virion and cell are referred to as biological target to be measured in test experience
(detection biotargets, DBTs).
If DBTs is large biological molecule, target biomolecule (target molecules, TMs) also known as to be measured.To list
The detection of individual biomolecule to be measured (TM), typically there is two kinds of technical schemes：
One is sandwich method, with known to one, the biomolecule (probe I) that can be specifically bound with TM combines
TM, add that another is known, be marked with detectable signal, can be specifically bound with TM or the-TM compounds of probe I
Biomolecule (probe II), formed-TM- probe II-detectable signal the compounds of probe I, separate and determine this compound
The power of middle detectable signal, the calibration curve between the detectable signal intensity and TM standard items contents that are determined according to experiment,
Check in contents of the TM in sample to be measured.Implement this method, it is necessary to which TM has the probe identification position that two or more is independent of each other
Point, or TM only one of which probe recognition sites but exist and another can recognize the probe of the compound of TM- probes I；
The second is competition law, is generally used for the situation of TM only one of which probe recognition sites, probe I is marked upper detectable
Signal, the standard items after the standard items reaction with TM and sample to be measured react respectively again with TM react, in being reacted by two
The detectable signal strength difference of TM standard items-probe I-detectable signal compound is formed, to calculate TM in sample to be measured
Content.
If DBTs is virion or cell, it can be sentenced by the detection to its one or more marker molecule
The virion to be measured that breaks is which kind of cell viral, to be measured is which kind of cell.Now, each marker molecule has only needed to a mark
There is the probe I of detectable signal.
In the above-mentioned technical solutions, for marking the detectable signal of bioprobe, it is known that have enzyme, quantum dot, chemistry
The species such as luminescent material, collaurum, electroselenium, radio isotope, fluorescein, due to the detection of different types of detection signal
Mode is different, can not be typically used in mixed way in one-time detection reaction, and same class detects signal often only one of which or several
Identifiable signal abundance so that one-time detection reaction may only detect one or several biological targets to be measured (DBTs).
The generation of disease, develop and lapse to, often influenceed and regulation by multifactor, therefore in biological study and disease
It is typically necessary in diagnosis while detecting multiple DBTs.In order to improve detection efficiency, saving sample and saving testing cost, be to grind
Study carefully and provide more analysis indexes with diagnosis, people, which have invented, synchronously to detect that a variety of DBTs' is multiple in one-time detection reaction
Detection technique, mainly has：
Microarray 1. (microarray) technology, also known as biochip (biochip) technology.The technology is from film hybridization technique
Develop, its basic fundamental thought is that the difference that the detection reaction for different TM is placed on into same detection reaction system is put down
Face position is carried out simultaneously.It is the probe I that be able to will be specifically bound from different TM that its basic fundamental, which is realized, and solid phase is fixed in an orderly manner
The diverse location of substrate or basement membrane, i.e. biochip；Testing sample is reacted with biochip, TMs present in sample
Just captured by the probe I in biochip on respective array site, capture TM biochip site because TM bridge joint is made
With the probe II of fluorescence labeling can be specifically bound；By detecting whether the different array microdots of biochip have fluorescence signal, just
It can determine whether there are corresponding target molecules in sample.Because high-density biochip point sample instrument can be pointed out greatly on substrate
In 400 points of dot matrix every square centimeter so that this technology can synchronously detect ten hundreds of target molecules, be genome
The research such as, proteomics provides high-throughout research method and instrument.But, sample and biochip in the technology
Reaction belongs to solid-liquid phase reaction, and steric hindrance is big, association reaction is slower, generally requires a few hours, it is difficult to meet the time limit require compared with
High medical science detection.
2.xMAP technologies, also known as liquid microarrays technology.The technology is developed from flow cytometry, its basic thought be by
The dot matrix liquefied of microarray technology, so as to reduce steric hindrance, acceleration detection reaction；Its basic fundamental realize be, with
Coating can be marked from different probes I with the fluorescein different from microsphere fluorescence characteristic on the plastic microsphere of different fluorescent characteristics
The probe II that can be specifically bound from different target molecules, by above-mentioned different plastic microsphere-probe I, probe II-fluorescein point
Son mixes the detection reagent of composition liquid phase, and after sample generation association reaction, the target molecule in sample will be with reagent
Middle correspondent probe is combined, and forms-TM- II-the luciferin complexes of probe of plastic microsphere-probe I, this compound is by possessing
The flow cytometer showed of two excitation (beam of laser is used to excite the fluorescence of microballoon, another beam to be used to excite the fluorescence molecule on probe II)
During instrument detection unit, instrument distinguishes different microballoons according to the fluorescent characteristic and forescatering light characteristic of microballoon, according to difference
Whether the fluorescent characteristic of probe II is had on microballoon simultaneously, to determine whether the microsphere surface has association reaction, so that counter push away sample
In have which detect target molecule.Although the target molecule quantity that xMAP technologies can be detected is much not as good as microarray technology simultaneously, it
The efficiency of association reaction has not only been obviously improved, and half-quantitative detection, the requirement detected closer to medical science can be realized.But,
Although this technology can analyze multiple detection molecules simultaneously, because it is based on flow cytometer detection technology, instrument detection unit is to body
It is thousands of in system to be analyzed one by one to millions of microballoons, although single sample can be completed in several minutes
Analysis, but be difficult to complete the detection of hundreds of sample in a short time, it is difficult to meet the detection need of clinical a large amount of samples
Ask.
3. π code technologies, the technology uses Micrometer-Nanometer Processing Technology, in diameter some tens of pm to hundreds of microns of oval dish
Different patterns are engraved on piece, to distinguish different disks, then on different disks dressing probe I, in magnetic material (such as
Super paramagnetic beads) on dressing probe II, different disk-probes I, magnetic material-probe II are constituted into detection reagent, with sample
It is combined after reaction, the target molecule in sample will be combined with correspondent probe in reagent, formation disk-I-target molecule of probe-
The compound of II-magnetic material of probe, is separated above-mentioned compound with the disk of association reaction does not occur using magnetic, so
Afterwards by micro-imaging and image recognition technology, it is determined that the species and number of disk that is reaction or having neither part nor lot in reaction are participated in, from
And calculate the target molecule species and content contained in sample.This technology can be in the different micropores of one piece of microwell plate to difference
Sample examinations, not only can detect multiple target molecules simultaneously in a detection architecture, can also complete within a short period of time
The detection of multiple samples, it is difficult the problem of sample is counted in detection hundred in a short time to solve xMAP technologies.But, this technology
Disk uses Micrometer-Nanometer Processing Technology, and cost is difficult to reduce, and disk easily forms in detection architecture and stacks, forms larger dry
Disturb.
It can be seen that, people are exploring Multiple detection technology always, and Multiple detection technology is also improved all the time, and can still not have at present
Have a kind of reliable, cost performance it is high, can be while realizing the technology of simple sample Multiple detection and the quick Multiple detection of many samples.
The content of the invention
For deficiencies of the prior art, the present invention provides a kind of particulate colourity clustering method, it is intended to
Realize simple sample Multiple detection and the quick Multiple detection of many samples；And its kit is correspondingly provided.
To achieve these goals, the technical solution adopted by the present invention is as follows：
A kind of particulate colourity clustering method, comprises the following steps：
S1：Build biological target detection reagent
Color particles of different colourities is coated with different bioprobes I respectively, color particles-biological spy is formed
The polymer of pin I, different color particles-polymer of bioprobe I is used for occurring specificity from different biological targets to be measured
Association reaction；M kinds color particles-mixed with polymers of bioprobe I is uniform, form biological target detection reagent, the life
Biological targets different from the M kinds in sample to be measured specific binding reaction can occur for thing target detection reagent respectively simultaneously；Its
In, M is >=1 natural number.
S2：Carry out biological detection reaction
The biological target detection reagent, sample to be measured, N kinds separation agent are added into reaction cup I, reaction is formed and is suspended
Liquid, carries out biological detection reaction, forms color particles-bioprobe I-biological target to be measured-separation agent conjugate；N is
>=1 natural number.
S3：Separate biological detection reaction product
All reaction products that will be generated in S2 steps, do not occur color particles-life of association reaction with detection reagent
The polymer of physical prospecting pin I is separated.
S4：Testing result judges
By color particles-polymer of bioprobe I of the participation biological respinse separated in step S3, according to coloured silk therein
Color particulate colourity carries out cluster counting, the one-to-one relationship set up according to the sub- colourity of color particles and biological target species,
The species and content of the biological target to be measured contained in statistic mixed-state sample.
Or, will in step S3 separate reaction product after remaining color particles-polymer of bioprobe I according to wherein
The sub- colourity of color particles carry out cluster counting, and with the species of all color particles-polymer of bioprobe I in reagent with
Quantity carries out difference operation, and the cluster for obtaining participating in color particles-polymer of bioprobe I of reaction in step S3 is counted, then
What is contained in the one-to-one relationship set up according to the sub- colourity of color particles and biological target species to be measured, statistics sample to be measured treats
Survey the species and content of biological target.
Further, the diameter of described color particles is not more than 100 μm.
Further, step S3 specific separation method is：All separation agents and color particles-life are adsorbed with needle
Physical prospecting pin I-biological target to be measured-separation agent conjugate, is placed in reaction cup II, adds bioprobe-biology to be measured
Target depolymerizing agent, reuses needle and adsorbs and abandon all magnetic components, leave color particles-polymer of bioprobe I in
In reaction cup II, then carry out step S4；
Or, using external magnetic field by all separation agents and color particles-bioprobe I-biological target to be measured-
Separation agent conjugate is adsorbed in reaction cup I, by color particles not combined with separation agent-polymer of bioprobe I
It is transferred to pipettor in reaction cup III, then carries out step S4.
Wherein, the effect of described bioprobe-biological target to be measured depolymerizing agent is separation agent is departed from color particles
Son-bioprobe I-biological target-separation agent to be measured, in order to follow-up detection.Conventional bioprobe-biological target to be measured
Depolymerizing agent is marked to include but is not limited to Types Below：
Acidity dissociation agent：PH value is 1.5~3, such as glycine solution.
Alkalescence dissociation agent：PH value is 10~12.5, such as triethylamine solution.
High salt dissociates agent：Such as the MgCl that concentration is 3~5mol/L2Solution, concentration is 5-10mol/L LiCl solution etc..
Zwitterionic detergent：Such as concentration is 0.5~2wt% SDS solution.
Decomposition agent：Such as the urea that concentration is 2~8mol/L, concentration is 2~5mol/L guanidine hydrochlorides, and concentration is 5~20wt%
Thiocyanates acid etc..
Organic solvent：Such as concentration is 25~50% ethylene glycols, and such as concentration is 5~20%r dioxane.
Further, color particles that step S4 is used, which clusters method of counting, is：Step S3 is obtained using filtering technique
Color particles-polymer deposits of bioprobe I on filter screen, make its in Monolayer Dispersion arrange；It is micro- that big visual field is carried out again
Colour is taken pictures, and gained photo extracts the colourity of all particulates, and carry out cluster counting according to colourity by image procossing；Ginseng
Reflect in sample to be measured the kind of biological target to be measured occur with the species number of color particles-polymer of bioprobe I of reaction
The kind of the colourity correspondence biological target to be measured of colored particulate and each in class number, color particles-polymer of bioprobe I
The content of the particulate number correspondence biological target to be measured of colourity.
Further, described biological target is antibody, antigen, part, acceptor, oligonucleotide fragment, cell, virion
With at least one of immune complex.
Described separation agent is with the coated magnetic material of bioprobe II that can specifically bind biological target to be measured
Material, the bioprobe III with the foregoing all biological targets of energy specific recognition-sub- compound of I-color particles of bioprobe are wrapped
At least one of magnetic material and the coated magnetic material of M kinds biological target to be measured of quilt.
Described bioprobe I, bioprobe II and bioprobe III is antibody, antigen, part, acceptor, oligonucleotides
At least one of fragment, peptide nucleic acid.
Bioprobe II is a kind of material that can be with target to be measured generation specific reaction, for example：In double-antibody sandwich
In method, target to be measured is antigen, and bioprobe I is a kind of antibody of target, and bioprobe II is another antibody of target.And
In dual-antigen sandwich method, target to be measured is antibody, and bioprobe I is the antigen for detecting target, and bioprobe II is also target
Antigen, and bioprobe I are identicals.
Bioprobe III is a kind of material that can be reacted with all bioprobes I-target to be measured polymer, example
Such as：Complement c1q, can be combined with immune complex；And for example：Bioprobe I is antigen, and target is detection antibody, due to all
It is human antibody, therefore what the Fc of antibody sections were just as, it is possible to it is used as bioprobe III with animal anti-human antibody.
Accordingly, color particles-bioprobe I-biological target to be measured-separation agent conjugate of reaction generation is coloured silk
Color particulate-bioprobe I-biological target to be measured-II-magnetic material of bioprobe, color particles-bioprobe I-to be measured
Biological target-III-magnetic material of bioprobe and/or color particles-bioprobe I-biological target to be measured-magnetic material knot
Compound.
A kind of particulate colourity clustering kit, including the biological target detection reagent that above-mentioned S1 is built.
Further, above-mentioned separation agent can also be included.
Further, in addition to bioprobe-biological target to be measured depolymerizing agent.
In this kit, biological target detection reagent, separation agent and/or bioprobe-biological target to be measured depolymerization
Agent is separate packaging.
The diameter of described color particles is not more than 100 μm.Color particles refers to be infected with the color that can distinguish
Microballoon, color can be that color, fluorescence are different or depth of same color is different.Microballoon can be natural polymer
Microballoon, such as spherex, albumin microsphere, gelatine microsphere, chitosan microball；Can also be synthetic polymer microballoon, it is such as poly-
Phenylethylene micro ball, polyacrylic acid microballoon, silicon dioxide microsphere etc..
The dyeing of color particles can also can be dyed with additional dyestuff during preparation.Such as Application No.
0213936.5 Chinese patent, Chinese patent of Application No. 20041003508.3 etc..
Compared with prior art, the present invention has the advantages that：
1st, up to tens thousand of kinds biological targets can be detected simultaneously in a detection reaction.The detection technique of the present invention is directed to shape
Analyzed into target biomolecule compound, due to up to 36000 kinds of colourity, can almost meet the institute of existing biomedical detection
Require.
2nd, the above-mentioned detection of up to hundreds of samples can be rapidly completed.The detection technique of the present invention, in a 384 hole microplates
On can carry out the multiple determination reaction of 384 samples simultaneously, interpretation of result uses particulate colourity auto-clustering analysis system,
The analysis of detection reaction can be automatically performed.While single Samples detection index high throughput analysis is realized, many samples can be completed
High flux is examined.
3rd, qualitative analysis can be carried out and can be quantitatively detected.Due to each target biomolecule respectively with a colourity
Spherical micro-particle and detection probe molecule are combined, and form target biomolecule compound, participate in the particulate number of reaction with
The content of biological target to be measured is proportionate, and can calculate the corresponding concentration for detecting target according to the counting for participating in reacting microballoon.
4th, detection sensitivity is good.In theory, as long as there is a target to be measured in sample to be measured, a corresponding coloured silk is just had
Color particulate is separated, therefore the theoretical value single molecules level of its sensitivity for analysis.
5th, cost is low.Due to color particles that the present invention is used, as long as its colourity is distinct, fluorescein is not
It is necessary, therefore the cost of dyestuff decreases than traditional technology.Meanwhile, without using unimolecule passage, generating laser and micro-
Process technology, can not only save great amount of cost, and be more easy to realize volume production.
Embodiment
The present invention is described in further detail with reference to specific embodiment.In the examples below, color particles
A diameter of 8 μm, and the optical microscope system true field of micro-imaging is diameter 6.36mm, 3 μm of optical resolution, system
Effective enlargement ratio is 4.44 times.
Embodiment one
The common unexpected Identification of the antibodies of particulate colourity clustering methodology human blood type
Human blood type accident antibody refers to after blood transfusion, gestation that body is directed to the blood group antigens itself not having or antigen is sub-
The immune antibody that type is produced, match is difficult when not only resulting in blood transfusion again, if the women of child-bearing age generate unexpected antibody, bosom
Fetus is also can result in when pregnant and occurs neonatal hemolytic disease.Therefore, unexpected Antibody screening is blood transfusion and pregnant with unexpected Identification of the antibodies
The important inspection content of inspection.At present, unexpected Antibody screening and unexpected Identification of the antibodies are, as two detection projects, antibody to be used first
Screening agent cell and sample to be measured are reacted, and have aggegation to show to have unexpected antibody, show zero accident antibody without aggegation；For
There is the situation of unexpected antibody, it is necessary to be reacted respectively with sample to be measured using one group of Identification of the antibodies agent cell, according to different reagents
Blood group antigens composition in the agglutinating reaction and agent cell of cell, it is to be directed to that unexpected antibody is inferred as solving equation
The antibody of blood group antigens.Because agent cell group is made up of the human red blood cells containing different rare blood group antigens, scarcity of resources, valency
Lattice are expensive, and the cumbersome, interpretation of result of detection is complicated, detection efficiency is extremely inefficient, needs a kind of method of simple and effective badly
Improve detection efficiency and detection quality.The present embodiment utilizes the multiple analyte detectability and mark of particulate colourity clustering methodology
This high flux detectability, realizes the common unexpected Identification of the antibodies high efficient detection of human blood type.Due to human erythrocyte's blood group antigens
Up to hundreds of, for ease of statement, the present embodiment is only with the anti-A2 of incidence highest, anti-B2, anti-D, anti-E, anti-e, anti-C, anti-c etc.
Exemplified by the identification of 7 kinds of unexpected antibody, step is implemented as follows：
S1：Prepare the unexpected Identification of the antibodies reagent of particulate colourity clustering methodology human blood type
By 7 kinds of color micro-spheres (chrominance component of colourity is respectively 10 °, 20 °, 30 °, 40 °, 50 °, 60 °, 70 °), tone is
10 ° of coating A2 antigens, the coating B2 antigens that tone is 20 °, the coating D antigens that tone is 30 °, the coating E that tone is 40 ° resist
Original, the coating e antigens that tone is 50 °, the coating C antigens that tone is 60 °, the coating c antigens that tone is 70 °, and with poly- second two
Alcohol close, obtain microballoon (10)-A2, microballoon (20)-B2, microballoon (30)-D, microballoon (40)-E, microballoon (50)-e, microballoon (60)-
C, microballoon (70)-c, according to 1:1:1:1:1:1:1 ratio is suspended in pH7.4 PBS, and the concentration of every kind of microballoon is about
100/microlitre, are made the unexpected main reagent of Identification of the antibodies (reagent I) of particulate colourity clustering methodology human blood type, be placed in 4 DEG C it is standby
With；
100 nanometers of magnetic bead is coated with mouse anti-human igg, polyethylene glycol is closed, suspension is in pH7.4 PBS
In, the concentration of magnetic bead-anti-human igg particle is about 10000/microlitre, particulate colourity clustering methodology human blood type is made unexpected
Identification of the antibodies separation agent (reagent II), be placed in 4 DEG C it is standby；
The glycine buffer that pH is 2.5 is routinely prepared, dissociation reagent (reagent III) is used as
Microballoon envelope antigen with reference to the report such as Chen Qilong, Liu Jiahui, Wang Xijia method (Beijing University of Chemical Technology's journal-from
Right science version, the 3rd phase of volume 41) carry out, method of the magnetic bead coating anti-human igg with reference to reports such as Guo Huifang, Zhang Wenhong, warm Chinese ilexes
(Journal of Immunology, the 5th phase of volume 22) is carried out.
S2：Detection reaction
Conventional 384 hole plastics microplates (per 120 microlitres of pore volume) are taken, different detection sample blood are added in different micropores
30 microlitres of slurry, adds 30 microlitres of reagent I made from S1 steps, 40 microlitres of reagent II, is combined within 10 minutes in 37 DEG C of standings
Reaction.
S3：Separate reaction product
All materials that can be adsorbed by magnetic field in each hole are transferred completely into the corresponding of another 384 hole microplate with electromagnetic needle
Kong Zhong, reacting hole is corresponded with hole is transferred to, and being transferred to each bottom hole portion in hole has the filter membrane of 7 microns of filter opening diameter；In hole is transferred to
III 100 microlitres of reagent is separately added into, dissociation reaction, 5 minutes reaction time are carried out under the conditions of 37 DEG C of shakings of healing；Electromagnetism is used afterwards
Pin sucks the material that each Kong Zhongneng is adsorbed by magnetic field.Pressurization filtration is carried out to being respectively transferred to hole, the color micro-sphere in hole is embedded in filter
On the net, in monolayer alignment.
S4：Detection and result judgement
Each filter membrane progress microballoon for being transferred to hole is focused on using the sub- cluster analysis system of color particles and takes pictures, analyze photo
In each microballoon colourity, and carry out differential counting according to colourity.If the counting of microballoon (10) is more than zero, show in the sample
There are anti-A2 antibody, and the number of microballoon (10) and the concentration positive correlation of anti-A2 antibody；If the counting of microballoon (20) is more than zero, table
There are anti-B2 antibody, and the number of microballoon (20) and the concentration positive correlation of anti-B2 antibody in the bright sample；If the meter of microballoon (30)
Number is more than zero, shows there is anti-D in the sample, and number and the concentration positive correlation of anti-D of microballoon (30)；If microballoon
(40) counting is more than zero, shows there is anti-E antibody in the sample, and the number of microballoon (40) and the concentration positive correlation of anti-E antibody；
If the counting of microballoon (50) is more than zero, show there is anti-e antibody in the sample, and the number of microballoon (50) and anti-e antibody is dense
Spend positive correlation；If the counting of microballoon (60) is more than zero, show there is anti-C antibody in the sample, and the number and anti-C of microballoon (60)
The concentration positive correlation of antibody；If the counting of microballoon (70) is more than zero, show there is anti-c antibody in the sample, and microballoon (70)
Number and the concentration positive correlation of anti-c antibody.The dose-effect relationship that detection target is counted with microballoon is determined by calibration curve.
The sub- cluster analysis system of color particles can be automated and hole-specifically analyzed to the hole that is transferred to of above-mentioned microplate, rapidly
Obtain blood group antibody qualification result outside 1 people's will to 384 samples to be measured.
It should be appreciated that the present embodiment, which is only used for description particulate colourity clustering methodology, is used for the unexpected Identification of the antibodies of human blood type
Implementation process, the human blood type antibody that can be detected is not limited to above-mentioned 7 kinds.
Embodiment two
Particulate colourity clustering methodology tumor-marker analyte detection
Tumour is common disease, frequently-occurring disease, seriously jeopardizes the life security of patient.Laboratory diagnosis be early detection tumour,
The important method of therapic opportunity is won for patient.Due to the diversity of tumour, generally require simultaneously to dozens of tumor markers
Joint-detection is carried out, tumour is found to wide spectrum.The present embodiment is detected using the multiple analyte of particulate colourity clustering methodology
Ability and sample high flux detectability, realize the high efficient detection of tumor markers.Due to the tumor markers having now found that
Up to tens of kinds, for ease of statement, the present embodiment is only with most common alpha-fetoprotein (AFP), carcinomebryonic antigen (CEA), neuron
Specificity olefinic alcohol enzyme (NSE), scaly epithelium cancer associated antigen (SCC-Ag), PSA (PSA), cell cutin
Said exemplified by the detections of 7 kinds of tumor markerses such as plain fragment antigen 21-1 (CYFRA21-1), carbohydrate antigen 242 (CA242)
It is bright, implement step as follows：
S1：Prepare particulate colourity clustering methodology tumor markers detection reagent
By 7 kinds of color micro-spheres of 8 μm of particle diameter (chrominance component of colourity is respectively 10 °, 20 °, 30 °, 40 °, 50 °, 60 °,
70 °), tone is 10 ° of coating mouse anti-human AFP antibody, tone is 20 ° the anti-human CEA antibody of coating mouse, the bag that tone is 30 °
By the anti-human SCC-Ag antibody of coating mouse that the anti-human NSE antibody of mouse, tone are 40 °, the anti-human PSA antibody of coating mouse that tone is 50 °,
Tone is 60 ° of the anti-human CYFRA-1 antibody of coating mouse, the anti-human CA242 antibody of coating mouse that tone is 70 °, and uses polyethylene glycol
Closing, obtains microballoon (10)-anti-AFP, microballoon (20)-anti-CEA, microballoon (30)-anti-NSE, microballoon (40)-anti-SCC-Ag, microballoon
(50)-anti-psa, microballoon (60)-anti-CYFRA21-1, microballoon (70)-CA242, according to 1:1:1:1:1:1:1 ratio is suspended in
In pH7.4 PBS, particulate colourity clustering methodology human blood type is made in/microlitre of concentration about 100 of every kind of microballoon
The unexpected main reagent of Identification of the antibodies (reagent I), be placed in 4 DEG C it is standby；
100 nanometers of magnetic bead is coated with rabbit anti-mouse igg, polyethylene glycol is closed, suspension is in pH7.4 PBS
In, the concentration of magnetic bead-anti-mouse IgG particles is about 10000/microlitre, and particulate colourity clustering methodology tumor markers is made
Detect separation agent (reagent II), be placed in 4 DEG C it is standby；
The glycine buffer that pH is 2.0 is routinely prepared, dissociation reagent (reagent III) is used as
Microballoon envelope antigen with reference to the report such as Chen Qilong, Liu Jiahui, Wang Xijia method (Beijing University of Chemical Technology's journal-from
Right science version, the 3rd phase of volume 41) carry out, magnetic bead is coated with methods of the anti-mouse IgG with reference to reports such as Guo Huifang, Zhang Wenhong, warm Chinese ilexes
(Journal of Immunology, the 5th phase of volume 22) is carried out.
S2：Detection reaction
Conventional 384 hole plastics microplates (per 120 microlitres of pore volume) are taken, different detection sample blood are added in different micropores
30 microlitres of slurry, adds 30 microlitres of reagent I made from S1 steps, 40 microlitres of reagent II, is combined within 10 minutes in 37 DEG C of standings
Reaction.
S3：Separate reaction product
All materials that can be adsorbed by magnetic field in each hole are transferred completely into the corresponding of another 384 hole microplate with electromagnetic needle
Kong Zhong, reacting hole is corresponded with hole is transferred to, and being transferred to each bottom hole portion in hole has the filter membrane of 7 microns of filter opening diameter；In hole is transferred to
III 100 microlitres of reagent is separately added into, dissociation reaction, 5 minutes reaction time are carried out under the conditions of 37 DEG C of shakings of healing；Electromagnetism is used afterwards
Pin sucks the material that each Kong Zhongneng is adsorbed by magnetic field.Pressurization filtration is carried out to being respectively transferred to hole, the color micro-sphere in hole is embedded in filter
On the net, in monolayer alignment.
S4：Detection and result judgement
Each filter membrane progress microballoon for being transferred to hole is focused on using the sub- cluster analysis system of color particles and takes pictures, analyze photo
In each microballoon colourity, and carry out differential counting according to colourity.If the counting of microballoon (10) is more than zero, show in the sample
There are AFP, and number and the AFP concentration positive correlation of microballoon (10)；If the counting of microballoon (20) is more than zero, show in the sample
There are CEA, and number and the CEA concentration positive correlation of microballoon (20)；If the counting of microballoon (30) is more than zero, show in the sample
There are NSE, and number and the NSE concentration positive correlation of microballoon (30)；If the counting of microballoon (40) is more than zero, show in the sample
There are SCC-Ag, and number and the SCC-Ag concentration positive correlation of microballoon (40)；If the counting of microballoon (50) is more than zero, show this
There are PSA, and number and the PSA concentration positive correlation of microballoon (50) in sample；If the counting of microballoon (60) is more than zero, show this
There are CYFRA21-1, and number and the CYFRA21-1 concentration positive correlation of microballoon (60) in sample；If the counting of microballoon (70)
More than zero, show there is CA242 in the sample, and number and the CA242 concentration positive correlation of microballoon (70).Detect target and microballoon
The dose-effect relationship of counting is determined by calibration curve.
The sub- cluster analysis system of color particles can be automated and hole-specifically analyzed to the hole that is transferred to of above-mentioned microplate, rapidly
Obtain the multi-tumor marker testing result of 1 to 384 sample to be measured.
It should be appreciated that the present embodiment is only used for describing implementation of the particulate colourity clustering methodology for tumor markers
Journey, the tumor markers that can be detected is not limited to above-mentioned 7 kinds.
Embodiment three
Particulate colourity clustering methodology blood donor infects examination
To avoid Transfusion Transmission disease, menses are carried out to blood donor's sample and propagate the state that pathogen examination is blood collecting and supplying industry
Border common practice, the pressure examination project of China's rules and regulations includes HIV-1 antibody, HIV-p24, HBsAg, HCV antibody and TP
6 kinds of Testing index such as antibody, blood station is generally required while repeating above-mentioned examination to dozens of or even hundreds of blood donors.This
Embodiment utilizes the multiple analyte detectability and sample high flux detectability of particulate colourity clustering methodology, and realization is donated blood
Person infects examination.For ease of statement, the representative of HIV-1 antibody, HCV-NS3 antibody are used as using HIV-GP41 antibody in the present embodiment
As the representative of HCV antibody, TP-47 antibody as the representative of syphilis antibody, detected with HBsAg and HIV-p24 simultaneously exemplified by, have
Body realizes that step is as follows：
S1：Prepare particulate colourity clustering methodology blood donor infection examination
By 8 μm of 5 kinds of color micro-spheres (chrominance component of colourity is respectively 10 °, 20 °, 30 °, 40 °, 50 °) of particle diameter, tone
For 10 ° of coating HIV-GP41 antigens, the coating HCV-NS3 antigens that tone is 20 °, the coating TP-47 antigens that tone is 30 °,
Tone is 40 ° of coating people AntiHIV1 RT activity-p24 antibody, the coating human anti-HBsAg antibody that tone is 50 °, and is closed with polyethylene glycol,
Obtain microballoon (10)-HIV-GP41, microballoon (20)-HCV-NS3, microballoon (30)-TP-47, microballoon (40)-AntiHIV1 RT activity-p24, microballoon
(50)-anti-HBsAg, according to 1:1:1:1:1 ratio is suspended in pH7.4 PBS, the concentration about 100 of every kind of microballoon
It is individual/microlitre, the unexpected main reagent of Identification of the antibodies (reagent I) of particulate colourity clustering methodology human blood type is made, be placed in 4 DEG C it is standby；
100 nanometers of magnetic bead is coated with C1Q subunit, polyethylene glycol is closed, suspension delays in pH7.4 PBS
In fliud flushing, the concentration of magnetic bead-C1q particles is about 10000/microlitre, particulate colourity clustering methodology human blood type is made unexpected
Identification of the antibodies separation agent (reagent II), be placed in 4 DEG C it is standby；
The glycine buffer that pH is 2.0 is routinely prepared, dissociation reagent (reagent III) is used as
Microballoon envelope antigen with reference to the report such as Chen Qilong, Liu Jiahui, Wang Xijia method (Beijing University of Chemical Technology's journal-from
Right science version, the 3rd phase of volume 41) carry out, method of the magnetic bead coating C1Q with reference to reports such as Guo Huifang, Zhang Wenhong, warm Chinese ilexes
(Journal of Immunology, the 5th phase of volume 22) is carried out.
S2：Detection reaction
Conventional 384 hole plastics microplates (per 120 microlitres of pore volume) are taken, different detection sample blood are added in different micropores
30 microlitres of slurry, adds 30 microlitres of reagent I made from S1 steps, 40 microlitres of reagent II, is combined within 10 minutes in 37 DEG C of standings
Reaction.
S3：Separate reaction product
All materials that can be adsorbed by magnetic field in each hole are transferred completely into the corresponding of another 384 hole microplate with electromagnetic needle
Kong Zhong, reacting hole is corresponded with hole is transferred to, and being transferred to each bottom hole portion in hole has the filter membrane of 7 microns of filter opening diameter；In hole is transferred to
III 100 microlitres of reagent is separately added into, dissociation reaction, 5 minutes reaction time are carried out under the conditions of 37 DEG C of shakings of healing；Electromagnetism is used afterwards
Pin sucks the material that each Kong Zhongneng is adsorbed by magnetic field.Pressurization filtration is carried out to being respectively transferred to hole, the color micro-sphere in hole is embedded in filter
On the net, in monolayer alignment.
S4：Detection and result judgement
Each filter membrane progress microballoon for being transferred to hole is focused on using the sub- cluster analysis system of color particles and takes pictures, analyze photo
In each microballoon colourity, and carry out differential counting according to colourity.If the counting of microballoon (10) is more than zero, show in the sample
There are AntiHIV1 RT activity-GP41 antibody, and number and the concentration positive correlation of AntiHIV1 RT activity-GP41 antibody of microballoon (10)；If microballoon (20)
Count and be more than zero, show there is HCV-Ab IgG-NS3 antibody in the sample, and the number and the concentration of HCV-Ab IgG-NS3 antibody of microballoon (20)
Positive correlation；If the counting of microballoon (30) be more than zero, show there is anti-TP-47 antibody in the sample, and the number of microballoon (30) with
The concentration positive correlation of anti-TP-47 antibody；If the counting of microballoon (40) is more than zero, show there is HIV-p24, and microballoon in the sample
(40) number and HIV-p24 concentration positive correlation；If the counting of microballoon (50) is more than zero, show there is HBsAg in the sample,
And number and the HBsAg concentration positive correlation of microballoon (50).Detect that target and the dose-effect relationship that microballoon is counted are true by calibration curve
It is fixed.
The sub- cluster analysis system of color particles can be automated and hole-specifically analyzed to the hole that is transferred to of above-mentioned microplate, rapidly
Obtain the infection screening results of 1 to 384 sample to be measured.
It should be appreciated that the present embodiment is only used for describing the reality that particulate colourity clustering methodology infects examination for blood donor
Process is applied, the infection mark that can be detected is not limited to above-mentioned 5 kinds.
Example IV
Particulate colourity clustering methodology human red blood cells Blood grouping
Human red blood cells blood group is the alloantigen of human erythrocyte surface, inputs the blood of blood group incompatibility, may cause
Haemolysis and unexpected antibody are produced, the life and fertility safety of critical patient, therefore, pedestrian must be all entered to blood donor and receptor
Erythrocyte blood type is detected.The erythrocyte blood type having now found that is up to hundreds of, and the present embodiment utilizes particulate colourity clustering
The multiple analyte detectability and sample high flux detectability of method, are just to realize the detection of a variety of blood groups in human red blood cells
In statement, the present embodiment exemplified by detecting Staphylococal Protein A, B antigens, D antigens, E antigens, e antigens, C antigens, c antigens simultaneously, specifically
Realize that step is as follows：
S1：Prepare particulate colourity clustering methodology human red blood cells Blood grouping reagent
By 7 kinds of color micro-spheres of 8 μm of particle diameter (chrominance component of colourity is respectively 10 °, 20 °, 30 °, 40 °, 50 °, 60 °,
70 °), tone is 10 ° of the anti-A antibody of coating, tone is 20 ° the anti-B antibody of coating, the coating Antibodies Against Rhesus D Antigen that tone is 30 °,
Tone is 40 ° of the anti-E antibody of coating, tone is 50 ° the anti-e antibody of coating, the anti-C antibody of coating that tone is 60 °, tone
For the anti-e antibody of 70 ° of coatings, and closed with polyethylene glycol, obtain microballoon (10)-anti-A, microballoon (20)-anti-B, microballoon (30)-
Anti- D, microballoon (40)-anti-E, microballoon (50)-anti-e, microballoon (60)-anti-C, microballoon (70)-anti-c, according to 1:1:1:1:1:1:1 ratio
Example is suspended in pH7.4 PBS, and particulate colourity clustering is made in/microlitre of concentration about 100 of every kind of microballoon
Legal person's blood group accident the main reagent of Identification of the antibodies (reagent I), be placed in 4 DEG C it is standby；
100 nanometers of magnetic bead is coated with phytohemagglutin phytolectin, polyethylene glycol is closed, suspension is in pH7.4 PBS
In, the concentration of magnetic bead-blood clotting crude granule is about 10000/microlitre, and particulate colourity clustering methodology human blood type is made and surprisingly resists
Body identification separation agent (reagent II), be placed in 4 DEG C it is standby；
With deionized water, dissociation reagent (reagent III) is used as
Microballoon envelope antigen with reference to the report such as Chen Qilong, Liu Jiahui, Wang Xijia method (Beijing University of Chemical Technology's journal-from
Right science version, the 3rd phase of volume 41) carry out, method of the magnetic bead coating hemagglutinin with reference to reports such as Guo Huifang, Zhang Wenhong, warm Chinese ilexes
(Journal of Immunology, the 5th phase of volume 22) is carried out.
S2：Detection reaction
Conventional 384 hole plastics microplates (per 120 microlitres of pore volume) are taken, different detection samples are added in different micropores
2% 30 microlitres of red blood cell physiological saline suspension, adds 30 microlitres of reagent I made from S1 steps, 40 microlitres of reagent II, in 37 DEG C
Standing is combined reaction in 10 minutes.
S3：Separate reaction product
All materials that can be adsorbed by magnetic field in each hole are transferred completely into the corresponding of another 384 hole microplate with electromagnetic needle
Kong Zhong, reacting hole is corresponded with hole is transferred to, and being transferred to each bottom hole portion in hole has the filter membrane of 7 microns of filter opening diameter；In hole is transferred to
III 100 microlitres of reagent is separately added into, dissociation reaction, 5 minutes reaction time are carried out under the conditions of 37 DEG C of shakings of healing；Electromagnetism is used afterwards
Pin sucks the material that each Kong Zhongneng is adsorbed by magnetic field.Pressurization filtration is carried out to being respectively transferred to hole, the color micro-sphere in hole is embedded in filter
On the net, in monolayer alignment.
S4：Detection and result judgement
Each filter membrane progress microballoon for being transferred to hole is focused on using the sub- cluster analysis system of color particles and takes pictures, analyze photo
In each microballoon colourity, and carry out differential counting according to colourity.If the counting of microballoon (10) is more than zero, show that the sample is red
There is Staphylococal Protein A on cell；If the counting of microballoon (20) is more than zero, show there are B antigens on the sample red blood cell；If microballoon (30)
Counting be more than zero, show there are D antigens on the sample red blood cell；If the counting of microballoon (40) is more than zero, show that the sample is red
There are E antigens on cell；If the counting of microballoon (50) is more than zero, show there are e antigens on the sample red blood cell；If microballoon (60)
Counting be more than zero, show there are C antigens on the sample red blood cell；If the counting of microballoon (70) is more than zero, show that the sample is red
There are c antigens on cell.
The sub- cluster analysis system of color particles can be automated and hole-specifically analyzed to the hole that is transferred to of above-mentioned microplate, rapidly
Obtain the human red blood cells Blood grouping result of 1 to 384 sample to be measured.
It should be appreciated that the present embodiment is only used for describing implementation of the particulate colourity clustering methodology for human red blood cells blood group
Process, the human red blood cells blood group that can be detected is not limited to above-mentioned 7 kinds.
The above embodiment of the present invention is only example to illustrate the invention, and is not the implementation to the present invention
The restriction of mode.For those of ordinary skill in the field, other can also be made not on the basis of the above description
With the change and variation of form.Here all embodiments can not be exhaustive.It is every to belong to technical scheme
Row of the obvious changes or variations amplified out still in protection scope of the present invention.
Claims (10)
1. a kind of particulate colourity clustering method, it is characterised in that comprise the following steps：
S1：Build biological target detection reagent
Color particles of different colourities is coated with different bioprobes I respectively, color particles-bioprobe I is formed
Polymer, different color particles-polymer of bioprobe I is used to specifically bind from different biological targets to be measured
Reaction；M kinds color particles-mixed with polymers of bioprobe I is uniform, form biological target detection reagent, the biological target
Biological targets different from the M kinds in sample to be measured specific binding reaction can occur for mark detection reagent respectively simultaneously；Wherein, M
For >=1 natural number；
S2：Carry out biological detection reaction
The biological target detection reagent, sample to be measured, N kinds separation agent are added into reaction cup I, reaction suspension is formed, enters
Row biological detection is reacted, and forms color particles-bioprobe I-biological target to be measured-separation agent conjugate；N is >=1
Natural number；
S3：Separate biological detection reaction product
All reaction products that will be generated in S2 steps, with not occurring the color particles of association reaction-biology in detection reagent
The polymer of probe I is separated；
S4：Testing result judges
By separated in step S3 participation biological respinse color particles-polymer of bioprobe I, it is micro- according to colour therein
Particle colourity carries out cluster counting, the one-to-one relationship set up according to the sub- colourity of color particles and biological target species, statistics
The species and content of the biological target to be measured contained in detection sample；
And/or, will in step S3 separate reaction product after remaining color particles-polymer of bioprobe I according to therein
The sub- colourity of color particles carries out cluster counting, and with the species and number of all color particles-polymer of bioprobe I in reagent
Amount carries out difference operation, and the cluster for obtaining participating in color particles-polymer of bioprobe I of reaction in step S3 is counted, then is pressed
What is contained in the one-to-one relationship set up according to the sub- colourity of color particles and biological target species to be measured, statistics sample to be measured is to be measured
The species and content of biological target.
2. particulate colourity clustering method according to claim 1, it is characterised in that：Described color particles
Diameter is not more than 100 μm.
3. particulate colourity clustering method according to claim 1, it is characterised in that：Step S3 specific separation side
Method is：All separation agents are adsorbed with needle and color particles-bioprobe I-biological target to be measured-separation agent is combined
Thing, is placed in reaction cup II, adds bioprobe-biological target to be measured depolymerizing agent, reuses needle and adsorbs and abandon institute
Be magnetic component, leaves color particles-polymer of bioprobe I in reaction cup II, then carry out step S4；
Or, using external magnetic field by all separation agents and color particles-bioprobe I-biological target-separation to be measured
Agent combinations are adsorbed in reaction cup I, and color particles not combined with separation agent-polymer of bioprobe I is moved
Liquid device is transferred in reaction cup III, then carries out step S4.
4. particulate colourity clustering method according to claim 1, it is characterised in that：It is colored micro- that step S4 is used
Particle clusters method of counting：Color particles for being obtained step S3 using filtering technique-polymer deposits of bioprobe I are existed
On filter screen, it is set to be arranged in Monolayer Dispersion；Carry out the micro- colour in big visual field again to take pictures, gained photo is extracted by image procossing
The colourity of all particulates, and carry out cluster counting according to colourity；Participate in color particles-polymer of bioprobe I of reaction
Species number reflect that the species number of biological target to be measured, color particles-polymer of bioprobe I occur in sample to be measured prizes
The corresponding biological target to be measured of the particulate number of kind and each colourity of the colourity correspondence biological target to be measured of color particulate
Content.
5. particulate colourity clustering method according to claim 1, it is characterised in that described biological target is anti-
Body, antigen, part, acceptor, oligonucleotide fragment, cell, at least one of virion and immune complex.
6. particulate colourity clustering method according to claim 5, it is characterised in that described separation agent is tool
Have can specifically bind biological target to be measured the coated magnetic material of bioprobe II, with can specific recognition it is foregoing all
The coated magnetic material of bioprobe III and M kinds biological target to be measured of biological target-sub- compound of I-color particles of bioprobe
Mark at least one of coated magnetic material.
7. particulate colourity clustering method according to claim 6, it is characterised in that described bioprobe I, raw
Physical prospecting pin II and bioprobe III are at least one of antibody, antigen, part, acceptor, oligonucleotide fragment, peptide nucleic acid.
8. a kind of particulate colourity clustering kit, it is characterised in that including the life built in S1 as claimed in claim 1
Thing target detection reagent.
9. particulate colourity clustering kit according to claim 8, it is characterised in that will also including such as right
Seek the separation agent described in 6.
10. particulate colourity clustering kit according to claim 8, it is characterised in that also including biology spy
Pin-biological target to be measured depolymerizing agent.
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