CN107190036A - Zymotechnique prepared by a kind of intestine membrane protein - Google Patents
Zymotechnique prepared by a kind of intestine membrane protein Download PDFInfo
- Publication number
- CN107190036A CN107190036A CN201610141745.9A CN201610141745A CN107190036A CN 107190036 A CN107190036 A CN 107190036A CN 201610141745 A CN201610141745 A CN 201610141745A CN 107190036 A CN107190036 A CN 107190036A
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- Prior art keywords
- concentration
- enzyme
- resin
- hours
- membrane protein
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/30—Extraction; Separation; Purification by precipitation
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Microbiology (AREA)
- Water Supply & Treatment (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- External Artificial Organs (AREA)
Abstract
The present invention relates to the extraction process of intestine membrane protein, and in particular to zymotechnique prepared by a kind of intestine membrane protein.Using casing waste liquid as raw material, by enzymolysis, resin adsorption, filtering obtains resin and filtrate respectively；Resin is scrubbed, elutes, precipitate, being dried to obtain WY 90493RD；Filtrate is concentrated, intestine membrane protein is dried to obtain.Advantage is perfect technology, and cost is low, and utilization rate of raw materials is high, and no waste, energy-saving and emission-reduction, production efficiency is greatly improved.
Description
Technical field
The present invention relates to the extraction process of intestine membrane protein, and in particular to zymotechnique prepared by a kind of intestine membrane protein.
Background technology
Conventional intestine membrane protein manufacture craft is not perfect enough, it is impossible to efficent use of resources, causes raw material using insufficient, also produces
Raw a large amount of waste water, cost increases, and efficiency is low.
The content of the invention
In order to solve the above problems, the invention provides zymotechnique prepared by a kind of intestine membrane protein, technical maturity, cost is low,
Efficiency high.
In order to achieve the above object, present invention employs following technical scheme：Zymotechnique prepared by a kind of intestine membrane protein, tool
Body is following steps：
（1）Enzymolysis：Bacillus licheniformis protease is first added in casing waste liquid, enzyme concentration is enzyme-added per 10L casing waste liquids
2g, it is 9 then to adjust the PH of mixture with alkali lye, is warming up to 60 DEG C, is incubated 2 hours, then adds water melon albumen
Enzyme, enzyme concentration is, per the 10L enzyme-added 2g of casing waste liquid, to be incubated 2 hours, is finally warming up to 70 DEG C, is incubated 2 minutes, uses
100 mesh stainless steel filtering nets are filtrated to get enzymolysis liquid；
（2）Absorption, filtering：By step（2）The enzymolysis liquid of gained, which is cooled to after 50 DEG C, adds resin, and stirring and adsorbing 10 is small
When, resin is filtered out with 80 mesh fiber cloth filter bags, filtrate is collected；
（3）Washing, elution：1. the fiber cloth bag for filling resin is put into clear water and cleaned, untill no-sundries；2. will
The aqueous salt solu-tion that resin concentration of the collection in fiber cloth bag is 10% 90 minutes, washes away the impurity such as albumen； ③
Then it is 18% three elution resins of saline solution with concentration, collects eluent；
（4）Precipitation：1. it is 1 by the volume ratio of alcohol and eluent：1 adds alcohol, is then stirred evenly with sticking plaster until going out
Untill existing particle, now alcohol concentration is 20%；2. supernatant is removed after precipitating 24 hours, WY 90493RD is collected and sinks
Form sediment；（5）Dry：By step（4）Obtained WY 90493RD is placed under 60 DEG C of temperature conditionss and dried 24 hours, obtains solid
Body, that is, obtain WY 90493RD；
（6）Concentration：By step（2）The filtrate of gained is concentrated inside vacuum decker, concentration volume to original 1/4
；（7）Dry：Liquid after concentration is spray-dried, wherein EAT is 160 DEG C, that is, obtains dried porcine saluble.
It is an advantage of the invention that perfect technology, cost is low, utilization rate of raw materials is high, no waste, energy-saving and emission-reduction, production efficiency
Greatly improve.
Embodiment
Embodiment 1
Zymotechnique prepared by a kind of intestine membrane protein, is characterized in particular in following steps：
（1）Enzymolysis：Bacillus licheniformis protease is first added in casing waste liquid, enzyme concentration is enzyme-added per 10L casing waste liquids
2g, it is 9 then to adjust the PH of mixture with alkali lye, is warming up to 60 DEG C, is incubated 2 hours, then adds water melon albumen
Enzyme, enzyme concentration is, per the 10L enzyme-added 2g of casing waste liquid, to be incubated 2 hours, is finally warming up to 70 DEG C, is incubated 2 minutes, uses
100 mesh stainless steel filtering nets are filtrated to get enzymolysis liquid；（2）Absorption, filtering：By step（2）The enzymolysis liquid of gained is cooled to 50
Resin is added after DEG C, stirring and adsorbing 10 hours is filtered out resin with 80 mesh fiber cloth filter bags, collect filtrate；（3）Wash
Wash, elute：1. the fiber cloth bag for filling resin is put into clear water and cleaned, untill no-sundries；2. it will collect in fibre
The aqueous salt solu-tion that resin concentration in dimension cloth bag is 10% 90 minutes, washes away the impurity such as albumen；3. then with dense
Three elution resins of saline solution for 18% are spent, eluent is collected；
（4）Precipitation：1. it is 1 by the volume ratio of alcohol and eluent：1 adds alcohol, is then stirred evenly with sticking plaster until going out
Untill existing particle, now alcohol concentration is 20%；2. supernatant is removed after precipitating 24 hours, WY 90493RD is collected and sinks
Form sediment；（5）Dry：By step（4）Obtained WY 90493RD is placed under 60 DEG C of temperature conditionss and dried 24 hours, obtains solid
Body, that is, obtain WY 90493RD；
（6）Concentration：By step（2）The filtrate of gained is concentrated inside vacuum decker, concentration volume to original 1/4
；（7）Dry：Liquid after concentration is spray-dried, wherein EAT is 160 DEG C, that is, obtains dried porcine saluble.
Claims (1)
1. a kind of zymotechnique prepared by intestine membrane protein, it is characterised in that following steps：
（1）Enzymolysis：Bacillus licheniformis protease is first added in casing waste liquid, enzyme concentration is enzyme-added per 10L casing waste liquids
2g, it is 9 then to adjust the PH of mixture with alkali lye, is warming up to 60 DEG C, is incubated 2 hours, then adds water melon albumen
Enzyme, enzyme concentration is, per the 10L enzyme-added 2g of casing waste liquid, to be incubated 2 hours, is finally warming up to 70 DEG C, is incubated 2 minutes, uses
100 mesh stainless steel filtering nets are filtrated to get enzymolysis liquid；
（2）Absorption, filtering：By step（2）The enzymolysis liquid of gained, which is cooled to after 50 DEG C, adds resin, and stirring and adsorbing 10 is small
When, resin is filtered out with 80 mesh fiber cloth filter bags, filtrate is collected；
（3）Washing, elution：1. the fiber cloth bag for filling resin is put into clear water and cleaned, untill no-sundries；2. will
The aqueous salt solu-tion that resin concentration of the collection in fiber cloth bag is 10% 90 minutes, washes away the impurity such as albumen； ③
Then it is 18% three elution resins of saline solution with concentration, collects eluent；
（4）Precipitation：1. it is 1 by the volume ratio of alcohol and eluent：1 adds alcohol, is then stirred evenly with sticking plaster until going out
Untill existing particle, now alcohol concentration is 20%；2. supernatant is removed after precipitating 24 hours, WY 90493RD is collected and sinks
Form sediment；（5）Dry：By step（4）Obtained WY 90493RD is placed under 60 DEG C of temperature conditionss and dried 24 hours, obtains solid
Body, that is, obtain WY 90493RD；
Concentration：By step（2）The filtrate of gained is concentrated inside vacuum decker, concentration volume to original 1/4；
（7）Dry：Liquid after concentration is spray-dried, wherein EAT is 160 DEG C, that is, obtains dried porcine saluble.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610141745.9A CN107190036A (en) | 2016-03-14 | 2016-03-14 | Zymotechnique prepared by a kind of intestine membrane protein |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610141745.9A CN107190036A (en) | 2016-03-14 | 2016-03-14 | Zymotechnique prepared by a kind of intestine membrane protein |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107190036A true CN107190036A (en) | 2017-09-22 |
Family
ID=59870687
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610141745.9A Withdrawn CN107190036A (en) | 2016-03-14 | 2016-03-14 | Zymotechnique prepared by a kind of intestine membrane protein |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107190036A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110973340A (en) * | 2019-11-19 | 2020-04-10 | 山川生物科技(武汉)有限公司 | Preparation method of dried intestine membrane protein powder |
| CN114271403A (en) * | 2022-01-07 | 2022-04-05 | 郑怡珺 | Goat casing proteolysis product, preparation method and application thereof |
-
2016
- 2016-03-14 CN CN201610141745.9A patent/CN107190036A/en not_active Withdrawn
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110973340A (en) * | 2019-11-19 | 2020-04-10 | 山川生物科技(武汉)有限公司 | Preparation method of dried intestine membrane protein powder |
| CN114271403A (en) * | 2022-01-07 | 2022-04-05 | 郑怡珺 | Goat casing proteolysis product, preparation method and application thereof |
| CN114271403B (en) * | 2022-01-07 | 2023-09-12 | 郑怡珺 | Goat casing proteolytic products, preparation method and application thereof |
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| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20170922 |
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| WW01 | Invention patent application withdrawn after publication |