CN107189937A - A kind of microbial detection device and microorganism detection method - Google Patents
A kind of microbial detection device and microorganism detection method Download PDFInfo
- Publication number
- CN107189937A CN107189937A CN201710242583.2A CN201710242583A CN107189937A CN 107189937 A CN107189937 A CN 107189937A CN 201710242583 A CN201710242583 A CN 201710242583A CN 107189937 A CN107189937 A CN 107189937A
- Authority
- CN
- China
- Prior art keywords
- sensing element
- reative cell
- microorganism
- grid
- detection device
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/26—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pH
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Toxicology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
Abstract
The invention discloses a kind of microbial detection device and microorganism detection method, including：Hair answers room, sample to be detected is provided with reative cell, sensing element is provided with below sample to be detected, sensing element connected with reative cell and with sample contact to be detected, sensing element is also electrically connected with detector, detector includes substrate, the grid being arranged on substrate, source electrode, drain electrode and the semiconductor layer of the dielectric layer being arranged on grid and setting on the dielectric layer, and sensing element is arranged on substrate and is electrically connected with grid.The present invention solves that existing microorganism detection method can not be detected in real time, detection time is long and cost is high, bio-compatibility is poor, the low problem of sensitivity.
Description
Technical field
The present invention relates to microculture and detection technique field, more particularly to a kind of microbial detection device and micro- life
Object detecting method.
Background technology
Microorganism is widely present in nature.The method used in the prior art that predominantly detects is microculture.
In incubation, sample is placed in medium component and the controlled environment of condition of culture, so as to promote microorganism to grow.
At present, there is multiple-microorganism culture apparatus in the market, and these devices can detect microorganism in sample in 12-48 hours
Presence, and any infectious microorganism of presence can be excluded in 5 days.In addition, by carrying out second incubation to sample
And experiment, these devices can identify the property of microorganism with the time of 12-48 hours again.However, needing quickly to obtain
The application scenario of testing result, the time span needed for above-mentioned detection method is completely unacceptable.
Accordingly, it would be desirable to develop a kind of microbial detection device and method, it can rapidly and accurately detect microorganism, and
Above mentioned problem of the prior art can be avoided.
The content of the invention
The present invention provides and aims to solve the problem that the above-mentioned problems in the prior art.Specifically, present invention seek to address that existing
There is microorganism detection method not detected in real time, detection time is long and cost is high, bio-compatibility is poor, sensitivity is low
The problems such as.
The invention provides a kind of microbial detection device, including：Hair, which is answered, is provided with test sample to be checked in room, the reative cell
This, is provided with sensing element below the sample to be detected, the sensing element connected with the reative cell and with it is described to be checked
Sample contact is surveyed, sensing element is also electrically connected with detector, and the detector includes substrate, the grid being arranged on substrate, set
Source electrode, drain electrode and the semiconductor layer of dielectric layer and setting on the dielectric layer on grid are put, the sensing element is arranged on
It is electrically connected on the substrate and with the grid.
It is preferred that the reative cell is arranged on the top of the sensing element.
It is preferred that the volume of the reative cell is dimensioned such that state of the single microbial in the reative cell changes
PH value change caused by becoming can be also detected, and cause the reative cell to accommodate microorganism and can prevent bigger again
Mammalian cell enters the reative cell.
It is preferred that the volume of the reative cell is in the range of 1 microlitre to 1 nanoliter.
It is preferred that the detector is organic film FET.
It is preferred that being additionally provided with controller on the dielectric layer, the controller is used to control the organic film field effect
The parameter of transistor is to adapt to different detection object and detection demand.
A kind of microorganism detection method, comprises the following steps：
Step 1：Sample to be detected is put into reative cell；
Step 2：Observing growth of the microorganism in nutrient solution causes the pH value of nutrient solution to change；
Step 3：Electric charge is produced by pH value variation monitoring sensing element；
Step 4：The electric charge of sensing element causes the charge inducing on grid, source electrode, drain electrode or semiconductor layer to redistribute；
Step 5：Charge inducing redistribution on grid, source electrode, drain electrode or semiconductor layer is made by electrode coupled capacitor
Changed with the potential between source electrode and drain electrode is caused；
Step 6：Potential change between source electrode and drain electrode causes the channel current of the organic film FET to be sent out
Changing；
Step 7：Determined to whether there is microorganism in nutrient solution according to the change curve of channel current and which kind of be present
Microorganism.
Compared to the prior art the present invention, the advantage is that：
The invention provides a kind of microbial detection device and microorganism detection method, microorganism can be monitored in real time
Cultivate and quickly determination microorganism whether there is and microorganism species；Itself have both signal conversion and enlarging function, be easy to
It is integrated with follow-up signal processing circuit；It is easy to miniaturization, can be used for preparing the chip that integrated level is high, detection function is complicated；
Using soluble organic semiconductor, insulating layer material, it can be processed and prepared by low temperature typography, and be conducive to selection resistance to
Hot poor, generalization backing material, so as to which cost and energy consumption is greatly reduced, improves production efficiency.
Brief description of the drawings
A kind of microbial detection device structural representation that Fig. 1 provides for the present invention.
Description of reference numerals：
10th, sample to be detected；11st, sensing element；12nd, substrate；13rd, grid；14th, dielectric layer；15th, controller；16th, leak
Pole；17th, source electrode；18th, reative cell；19th, semiconductor layer.
Embodiment
Below in conjunction with the accompanying drawings, the specific embodiment of the present invention is described in detail, it is to be understood that of the invention
Protection domain do not limited by embodiment.
As shown in figure 1, the embodiments of the invention provide a kind of microbial detection device, including：Hair answers room 18, the reaction
Sample 10 to be detected is provided with room 18, the lower section of sample 10 to be detected is provided with sensing element 11, the sensing element 11 and institute
State reative cell 11 to connect and contact with the sample 10 to be detected, sensing element 11 is also electrically connected with detector, the detection
Instrument includes substrate 12, sets grid 13 on the substrate 12, the dielectric layer 14 being arranged on grid 13 and be arranged on dielectric layer
Source electrode 17 on 14, drain electrode 16 and semiconductor layer 19, the sensing element 11 set on the substrate 12 and with the grid
Pole 13 is electrically connected.Detection means of the present invention, can monitor in real time microorganism culture and quickly determine microorganism whether there is with
And the species of microorganism；Itself have both signal conversion and enlarging function, be easy to integrated with follow-up signal processing circuit；It is easy to micro-
Type, can be used for preparing the chip that integrated level is high, detection function is complicated；Utilize soluble organic semiconductor, insulating barrier material
Material, can be processed by low temperature typography and prepared, and be conducive to selection poor heat resistance, the backing material of generalization, so as to
So that cost and energy consumption is greatly reduced, production efficiency is improved.
Further, the reative cell 18 is arranged on the top of the sensing element 11.
Further, the volume of the reative cell 18 is dimensioned such that single microbial in the reative cell 18
PH value change caused by state change can be also detected, and make it that the reative cell 18 can accommodate microorganism and can prevent
Only bigger mammalian cell enters the reative cell 18.
Further, the volume of the reative cell 18 is in the range of 1 microlitre to 1 nanoliter.
Further, the detector is organic film FET.
Further, controller 15 is additionally provided with the dielectric layer 14, the controller 15 is described organic thin for controlling
The parameter of film field-effect transistor is to adapt to different detection object and detection demand.
A kind of microorganism detection method, comprises the following steps：
Step 1：Sample to be detected is put into reative cell；
Step 2：Observing growth of the microorganism in nutrient solution causes the pH value of nutrient solution to change；
Step 3：Electric charge is produced by pH value variation monitoring sensing element；
Step 4：The electric charge of sensing element causes the charge inducing on grid, source electrode, drain electrode or semiconductor layer to redistribute；
Step 5：Charge inducing redistribution on grid, source electrode, drain electrode or semiconductor layer is made by electrode coupled capacitor
Changed with the potential between source electrode and drain electrode is caused；
Step 6：Potential change between source electrode and drain electrode causes the channel current of the organic film FET to be sent out
Changing；
Step 7：Determined to whether there is microorganism in nutrient solution according to the change curve of channel current and which kind of be present
Microorganism.
In summary, a kind of microbial detection device and microorganism detection method provided in an embodiment of the present invention, can be with
In real time monitoring microorganism culture and quickly determination microorganism whether there is and microorganism species；Itself have both signal conversion
And enlarging function, it is easy to integrated with follow-up signal processing circuit；It is easy to miniaturization, can be used for preparing integrated level height, detection
The complicated chip of function；Using soluble organic semiconductor, insulating layer material, it can be processed and made by low temperature typography
It is standby, and be conducive to selection poor heat resistance, the backing material of generalization, so as to which cost and energy consumption is greatly reduced, improve
Production efficiency.
Disclosed above is only several specific embodiments of the present invention, and still, the embodiment of the present invention is not limited to this, is appointed
What what those skilled in the art can think change should all fall into protection scope of the present invention.
Claims (7)
1. a kind of microbial detection device, it is characterised in that including：Hair is answered in room (18), the reative cell (18) provided with to be checked
Sensing element (11), the sensing element (11) and the reaction are provided with below test sample sheet (10), the sample (10) to be detected
Room (11) connects and contacted with the sample (10) to be detected, and sensing element (11) is also electrically connected with detector, the detection
Instrument includes substrate (12), the grid (13) being arranged on substrate (12), dielectric layer (14), the Yi Jishe being arranged on grid (13)
Source electrode (17), drain electrode (16) and semiconductor layer (19) on dielectric layer (14) are put, the sensing element (11) is arranged on described
It is electrically connected on substrate (12) and with the grid (13).
2. a kind of microbial detection device as claimed in claim 1, it is characterised in that the reative cell (18) is arranged on described
The top of sensing element (11).
3. a kind of microbial detection device as any one of claim 1 to 2, it is characterised in that the reative cell
(18) volume is dimensioned such that the pH value caused by state change of the single microbial in the reative cell (18) becomes
Change can be also detected, and cause the reative cell (18) to accommodate microorganism and can prevent bigger mammalian cell again
Into the reative cell (18).
4. a kind of microbial detection device as claimed in claim 3, it is characterised in that the volume of the reative cell (18) is 1
Microlitre in the range of 1 nanoliter.
5. a kind of microbial detection device as claimed in claim 1, it is characterised in that the detector is that organic film field effect is brilliant
Body pipe.
6. a kind of microbial detection device as claimed in claim 5, it is characterised in that be additionally provided with control on the dielectric layer (14)
Device (15), the controller (15) is used to control the parameter of the organic film FET to adapt to different detections pair
As and detection demand.
7. a kind of microorganism detection method, it is characterised in that comprise the following steps：
Step 1：Sample to be detected is put into reative cell；
Step 2：Observing growth of the microorganism in nutrient solution causes the pH value of nutrient solution to change；
Step 3：Electric charge is produced by pH value variation monitoring sensing element；
Step 4：The electric charge of sensing element causes the charge inducing on grid, source electrode, drain electrode or semiconductor layer to redistribute；
Step 5：Charge inducing redistribution on grid, source electrode, drain electrode or semiconductor layer is drawn by the effect of electrode coupled capacitor
The potential risen between source electrode and drain electrode changes；
Step 6：Potential change between source electrode and drain electrode causes the channel current of the organic film FET to become
Change；
Step 7：Which kind of determined according to the change curve of channel current in nutrient solution with the presence or absence of microorganism and in the presence of micro- life
Thing.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710242583.2A CN107189937A (en) | 2017-04-14 | 2017-04-14 | A kind of microbial detection device and microorganism detection method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710242583.2A CN107189937A (en) | 2017-04-14 | 2017-04-14 | A kind of microbial detection device and microorganism detection method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107189937A true CN107189937A (en) | 2017-09-22 |
Family
ID=59870893
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710242583.2A Pending CN107189937A (en) | 2017-04-14 | 2017-04-14 | A kind of microbial detection device and microorganism detection method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107189937A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110534478A (en) * | 2018-05-24 | 2019-12-03 | 中国科学院苏州纳米技术与纳米仿生研究所 | Senser element and preparation method thereof |
| CN113333042A (en) * | 2021-06-21 | 2021-09-03 | 太原理工大学 | Micro-fluidic chip for nucleic acid detection and manufacturing method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104502427A (en) * | 2015-01-07 | 2015-04-08 | 融智生物科技（青岛）有限公司 | Microbiological detection device and microbiological detection method |
| CN204422470U (en) * | 2015-01-07 | 2015-06-24 | 融智生物科技（青岛）有限公司 | Microbial detection device |
-
2017
- 2017-04-14 CN CN201710242583.2A patent/CN107189937A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104502427A (en) * | 2015-01-07 | 2015-04-08 | 融智生物科技（青岛）有限公司 | Microbiological detection device and microbiological detection method |
| CN204422470U (en) * | 2015-01-07 | 2015-06-24 | 融智生物科技（青岛）有限公司 | Microbial detection device |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110534478A (en) * | 2018-05-24 | 2019-12-03 | 中国科学院苏州纳米技术与纳米仿生研究所 | Senser element and preparation method thereof |
| CN113333042A (en) * | 2021-06-21 | 2021-09-03 | 太原理工大学 | Micro-fluidic chip for nucleic acid detection and manufacturing method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CA2859914C (en) | Methods and apparatus for rapid detection of infectious microorganisms | |
| CN107870004A (en) | flexible sensing device based on liquid metal | |
| CN104965080B (en) | A kind of reaction member for detection antibody or antigen and system | |
| CN107189937A (en) | A kind of microbial detection device and microorganism detection method | |
| CN102559483B (en) | Device and method for real-time monitoring of behaviors and states of cells | |
| US11384330B2 (en) | Electrochemical sensors for cell culture monitoring | |
| CN103941171B (en) | Semiconductor test structure and test method | |
| CN204422470U (en) | Microbial detection device | |
| CN108387624B (en) | Three-dimensional porous carbon/polysulfide cordierite compound modified electrode and preparation and application thereof | |
| Parsnejad et al. | Wide dynamic range multi-channel electrochemical instrument for in-field measurements | |
| CN106328659A (en) | Array substrate and preparation method thereof and display panel | |
| CN104502427A (en) | Microbiological detection device and microbiological detection method | |
| CN103289896B (en) | Field effect tissue culture dish system and application | |
| CN105044163A (en) | Device and method for on-line detection of microbial fermentation ethanol concentration | |
| CN215328113U (en) | Nucleic acid detection equipment based on deep learning | |
| Hofmann et al. | Galvanic decoupled sensor for monitoring biomass concentration during fermentation processes | |
| TW468233B (en) | Apparatus and measurement method of hysteresis and time shift for ISFET containing amorphous silicon hydride sensing membrane | |
| TW201226896A (en) | Microbe or cell inspection system and method thereof | |
| CN204925008U (en) | Microbial fermentation ethanol concentration on -line measuring device | |
| CN105136890A (en) | N-doped graphene biosensor | |
| Cathcart et al. | Transparent thin film transistor electrode array for real-time electrical impedance spectroscopy of cell cultures | |
| CN114235913B (en) | Heating incubation method and heating incubation system of biological detection chip | |
| CN112175785B (en) | Dual-channel isothermal amplification device and method | |
| US20180024688A1 (en) | An apparatus and methods for sensing | |
| CN115963160B (en) | Multi-parameter trans-scale biochemical sensor chip and application method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170922 |
|
| RJ01 | Rejection of invention patent application after publication |