CN107184620A - The application of mysterious berry extract - Google Patents

The application of mysterious berry extract Download PDF

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Publication number
CN107184620A
CN107184620A CN201710322530.1A CN201710322530A CN107184620A CN 107184620 A CN107184620 A CN 107184620A CN 201710322530 A CN201710322530 A CN 201710322530A CN 107184620 A CN107184620 A CN 107184620A
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solution
extract
berry extract
application according
mysterious berry
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马飞跃
袁晓丽
张秀梅
刘玉革
付琼
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South Subtropical Crops Research Institute CATAS
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105—Plant extracts, their artificial duplicates or their derivatives
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/08—Anti-ageing preparations
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/10—Preparation or pretreatment of starting material
    • A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
    • A61K2800/805—Corresponding aspects not provided for by any of codes A61K2800/81 - A61K2800/95

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Mycology (AREA)
  • Botany (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Chemical & Material Sciences (AREA)
  • Epidemiology (AREA)
  • Gerontology & Geriatric Medicine (AREA)
  • Medicinal Chemistry (AREA)
  • Medical Informatics (AREA)
  • Alternative & Traditional Medicine (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Dermatology (AREA)
  • Nutrition Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Birds (AREA)
  • Cosmetics (AREA)
  • Medicines Containing Plant Substances (AREA)

Abstract

The present invention relates to a kind of application of mysterious berry extract in preparing for anti-aging or strengthening medicine, food or the cosmetics of oxidative stress, the mysterious berry extract is extracted by following steps：Negative pressure cavitation extraction is carried out to miracle fruit pulp with solution is extracted, extract solution is obtained；After the obtained extract solution is concentrated, separated through macroporous absorbent resin, collect eluent；The eluent through being concentrated under reduced pressure, is freeze-dried again, obtains mysterious berry extract；The solution that extracts is the enzyme solutions that pH is 1.5~5.5, and the solution and the liquid-solid ratio of the miracle fruit pulp of extracting is 5~20mL/g.The mysterious berry extract anti-aging effects effect substantially, and has significant biological safety, so as to for preparing for anti-aging or strengthening medicine, food or the cosmetics of oxidative stress.

Description

The application of mysterious berry extract
Technical field
The present invention relates to Natural Medicine Chemistry and biological technical field, in particular it relates to which mysterious berry extract prolongs in preparation Application in medicine, food or the cosmetics of slow aging or enhancing oxidative stress effect.
Background technology
Miracle fruit (Synsepalum dulcificum Daniell) category Sapotaceae miracle fruit category, though originate in West African fever Band area, is planted extensively in China tropical and subtropical region.Miracle fruit pulp contains abundant peculiar glycoprotein, dimension life The nutriments such as element, organic acid, flavones, anthocyanidin.The research for miracle fruit complete stool shows at this stage, and it has regulation blood Pressure, blood fat, the effects such as mitigating gout, uric acid, liver-protecting sobering up.
C. Elegans Automatic Screening is as the hotspot pattern of aging research in the world is biological and its normalizing operation of aging related experiment Valuable information platform is provided for the Mechanism Study of natural products anti-aging.The glp-1 of C. Elegans Automatic Screening is N- glycosyls Change transmembrane protein, encode sexual gland cell of termination (distal tip cell/DTC) reproduction proliferation signal acceptor, determine reproduction cell Destiny is reproduction cell mitosis and safeguards the basis of germline stem cell, relevant with nematode life.Inventor utilizes god Secret berry extract feeds Caenorhabditis elegans (C.elegans), studies whether it has raising nematode anti-aging and enhancing oxidation Stress ability, and then signal path is studied, discloses mysterious berry extract anti-aging and strengthen the mechanism of oxidative stress, So as to can be safely used for medicine, food and cosmetic field.
The content of the invention
Preparing anti-aging it is an object of the invention to provide mysterious berry extract or strengthening the medicine of oxidative stress, food Application in product or cosmetics.
According to an aspect of the present invention anti-aging or enhancing oxidative stress are being prepared there is provided a kind of mysterious berry extract Medicine, food or cosmetics in application, wherein, the mysterious berry extract is extracted by following steps：
Negative pressure cavitation extraction is carried out to miracle fruit pulp with solution is extracted, extract solution is obtained；
After the obtained extract solution is concentrated, separated through macroporous absorbent resin, collect eluent；
The eluent through being concentrated under reduced pressure, is freeze-dried again, obtains mysterious berry extract；
The solution that extracts is the enzyme solutions that pH is 1.5~5.5, and the liquid for extracting solution and the miracle fruit pulp Gu than being 5~20mL/g.
According to an embodiment, the enzyme is cellulase, pectase, or the fiber that mass ratio is 1: 2~6: 1 The complex enzyme of plain enzyme and pectase composition.Preferably, the enzyme and the miracle fruit pulp amount ratio are 1.0~8.0mg/ g。
According to another embodiment, the extraction solution organic acid, such as glacial acetic acid or lemon acid for adjusting pH.
According to further embodiment, the Extracting temperature that the negative pressure cavitation is extracted is 20 DEG C~55 DEG C, extract pressure for- 0.04MPa~-0.07MPa.
According to an embodiment, the macroporous absorbent resin is selected from HP 2MGL, HP-20, ADS-5, ADS-8, ADS- 17th, D3520, D4020, D14, D16 type macroporous absorbent resin.
Preferably, the ethanol water that the eluent in the macroporous absorbent resin separation is 60~90% (v/v).
Ethanol first with 10~20% (v/v) before being eluted with 60~90% (v/v) ethanol water is water-soluble Liquid is eluted.
The mysterious berry extract relies on germ cell line (germ line) signal path and realizes anti-aging or enhancing oxygen Change stress.
The mysterious berry extract is 0.001~0.01mg/mL to the useful effect concentration of nematode.
It is obvious according to the mysterious berry extract anti-aging effects effect of the present invention, and there is significant biological safety, from And available for medicine, food or the cosmetics prepared for anti-aging or enhancing oxidative stress.
Brief description of the drawings
Fig. 1 shows the UPLC chromatograms of the mysterious berry extract according to the present invention detected under 325nm wavelength.
Fig. 2 shows the influence to the nematode life-span according to the mysterious berry extract of the present invention.
Fig. 3 is shown extends the nematode longevity according to the mysterious berry extract of the present invention dependent on germ cell line signal path Life.
Fig. 4 shows the mysterious berry extract according to the present invention to nematode H2O2It is resistance to affected.
Embodiment
The invention provides a kind of mysterious berry extract prepare anti-aging or strengthen the medicine of oxidative stress, food or Application in cosmetics, wherein, the mysterious berry extract is extracted by following steps：
Negative pressure cavitation extraction is carried out to miracle fruit pulp with solution is extracted, extract solution is obtained；
After the obtained extract solution is concentrated, separated through macroporous absorbent resin, collect eluent；
The eluent through being concentrated under reduced pressure, is freeze-dried again, obtains mysterious berry extract.
Wherein, to extract solution be the enzyme solutions that pH is 1.5~5.5, and solution and the miracle fruit pulp of extracting Liquid-solid ratio is 5~20mL/g.
According to an embodiment, the enzyme in the enzyme solutions is cellulase, pectase, or mass ratio is 1: 2~6: 1 The cellulase and the pectase composition complex enzyme.Preferably, the enzyme is with the miracle fruit pulp amount ratio 1.0~8.0mg/g.
According to another embodiment, the organic acid for adjusting pH for extracting solution with such as glacial acetic acid or citric acid.
According to further embodiment, the Extracting temperature that the negative pressure cavitation is extracted is 20 DEG C~55 DEG C, extract pressure for- 0.04MPa~-0.07MPa.
Above-mentioned negative pressure cavitation extracts the continuous extraction of preferred progress 1~3 time, every time 15~35min.
Preferably, before macroporous absorbent resin separation is carried out, gained extract solution is concentrated under reduced pressure into Extraction solvent used The 1/7~1/5 of cumulative volume.
According to a specific embodiment, the macroporous absorbent resin be selected from HP 2MGL, HP-20, ADS-5, ADS-8, ADS-17, D3520, D4020, D14, D16 type macroporous absorbent resin.
Preferably, the ethanol water that the eluent in the macroporous absorbent resin separation is 60~90% (v/v).
Ethanol first with 10~20% (v/v) before being eluted with 60~90% (v/v) ethanol water is water-soluble Liquid is eluted.
Above-mentioned mysterious berry extract relies on germ cell line signal path and realizes anti-aging or enhancing oxidative stress effect.
Described macroporous adsorbing resin for purification isolation technics, big pore adsorption resin is selected from HP 2MGL, HP-20, ADS- 5th, ADS-8, ADS-17, D3520, D4020, D14, D16 type macroporous absorption tree.The blade diameter length ratio of above-mentioned chromatographic resin post is 1: 5~1 : 20, loading volume is 2~6BV, and loading flow velocity is 1.5~4BV/h, with 3~8BV/h flow velocity with 5~7BV after adsorption equilibrium Water elution is sweetened off, and the acidic ethanol of 4~10BV50%~60% elution, gained elutes solution through being concentrated under reduced pressure, after freeze-drying Miracle fruit fruit extract.
The preparation of mysterious berry extract
Embodiment 1
Stoning miracle fruit pulp 400g is weighed, it is 6 to add mass ratio:1 cellulase and the aqueous solution of pectase, use lemon Lemon acid adjusts the aqueous solution to pH 3.5.Enzyme is 5.0mg/g with miracle fruit pulp amount ratio, carries out negative pressure cavitation extraction.Carry The liquid-solid ratio for taking enzyme aqueous solution and miracle fruit pulp is 10mL/g.Extracting temperature is 50 DEG C, continuously extraction 3 times, every time 25min；Extract pressure -0.04MPa.
Gained extract solution is concentrated under reduced pressure into the 1/7 of Extraction solvent cumulative volume used.Then, HP-20 macroporous absorption trees are utilized Fat carries out concentration and separation.Wherein, the blade diameter length ratio of chromatographic resin post is 1: 20, and loading volume is 4BV, and loading flow velocity is 2BV/h.Inhale Sugar is sloughed with 5BV 10% (v/v) ethanol aqueous wash with 5BV/h flow velocity after attached balance, then uses 8BV60% acidic ethanols The aqueous solution is eluted, and collects the eluent.
Gained eluent obtains mysterious berry extract 41.37mg through being concentrated under reduced pressure after being freeze-dried in -45 DEG C.
Embodiment 2
Stoning miracle fruit pulp 400g is weighed, it is 1 to add mass ratio:2 cellulase and the aqueous solution of pectase, use lemon Lemon acid adjusts the aqueous solution to pH 2.Enzyme is 8.0mg/g with miracle fruit pulp amount ratio, carries out negative pressure cavitation extraction.Extract Liquid-solid ratio with enzyme aqueous solution and miracle fruit pulp is 20mL/g.Extracting temperature is 55 DEG C, continuous to extract 3 times, each 25min； Extract pressure -0.06MPa.
Gained extract solution is concentrated under reduced pressure into the 1/5 of Extraction solvent cumulative volume used.Then, D3520 macroporous absorption trees are utilized Fat carries out concentration and separation.Wherein, the blade diameter length ratio of chromatographic resin post is 1: 20, and loading volume is 4BV, and loading flow velocity is 2BV/h.Inhale Sugar is sloughed with 5BV 15% (v/v) ethanol aqueous wash with 5BV/h flow velocity after attached balance, then uses 8BV60% acidic ethanols The aqueous solution is eluted, and collects the eluent.
Gained eluent obtains miracle fruit fruit extract 43.62mg through being concentrated under reduced pressure after being freeze-dried in -45 DEG C.
Embodiment 3
Stoning miracle fruit pulp 400g is weighed, it is 3: 1 cellulase and the aqueous solution of pectase to add mass ratio, uses lemon Lemon acid adjusts the aqueous solution to pH 5.5.Enzyme is 8.0mg/g with miracle fruit pulp amount ratio, carries out negative pressure cavitation extraction.Carry The liquid-solid ratio for taking enzyme aqueous solution and miracle fruit pulp is 5mL/g.Extracting temperature is 20 DEG C, continuous to extract 3 times, each 25min； Extract pressure -0.07MPa.
Gained extract solution is concentrated under reduced pressure into the 1/5 of Extraction solvent cumulative volume used.Then, D3520 macroporous absorption trees are utilized Fat carries out concentration and separation.Wherein, the blade diameter length ratio of chromatographic resin post is 1: 20, and loading volume is 4BV, and loading flow velocity is 2BV/h.Inhale Sugar is sloughed with 5BV 20% (v/v) ethanol aqueous wash with 5BV/h flow velocity after attached balance, then uses 8BV60% acidic ethanols The aqueous solution is eluted, and collects the eluent.
Gained eluent obtains miracle fruit fruit extract 42.09mg through being concentrated under reduced pressure after being freeze-dried in -45 DEG C.
Constituent analysis in mysterious berry extract
Using the polyphenol composition in the above-mentioned target product of high effective liquid chromatography for measuring.Wherein, high performance liquid chromatography (HPLC) condition is：
Supelco (Bellefonte, PA) Discovery C18 octadecyl solid-phase extraction columns, 35 DEG C of column temperature；
Waters2690 separator units；
Water996 photodiode detectors；
The Chromatography Manager softwares of Waters Millennium 32；
Mobile phase is the mixed liquor of acetonitrile and 10% acetic acid aqueous solution, and flow velocity is 0.8ml/min, using gradient elution；
Detection wavelength is 370nm and 260nm.
The composition in target product is determined according to standard specimen.
Target product by acetonitrile and acetic acid water gradient separations after HPLC see Fig. 1, by result understand miracle fruit pulp Main active substances in extract are：
1st, vitamin C 2, gallic acid 3, catechuic acid 4, epicatechin 5, ellagic acid 6, rutin 7, myricetin 8, Quercetin 9, kaempferol.
The experiment of mysterious berry extract anti-aging performance
Animal model of the present invention using C. Elegans Automatic Screening as research anti-aging, by mutator nematode, observes nematode Survival rate and the life-span, the signal path of the mysterious berry extract anti-aging of checking.
1st, nematode life experiment is extended
Nematode：Wild type nematode N2 (Bristol strain), buys from Shanghai model biotech inc.
Experiment material：The mysterious berry extract as made from embodiment 1-3；0.1%DMSO, Shanghai model biotechnology share Co., Ltd.
Experimental method：
(1) 10 are put into each 60mm culture dish containing NGM (nematode growth medim) culture medium Have in vivo after the adult of egg, 30min, adult is chosen, synchronizing, totally 3 culture dishes.Cultivation temperature is 20 DEG C, humidity 40~60%.
After (2) 3 days, the hermaphroditic nematode developed for adult is being scribbled OP50 and deoxygenated containing 400uM 5- fluorine Cultivate, contain in NGM culture mediums on the NGM culture mediums of uridine (5-fluoro-2 '-deoxyuridine, FUDR, Sigma) 12.5ug/mL (or 50uM) FUDR.
(3) 3 treatment groups are respectively mysterious berry extract prepared by embodiment 1~3, and dosage is 10ug/mL, spirit Sesame is positive control (0.1mg/mL), and blank control is without any processing, treatment group and each 10 wares of control group, and record is dead daily Die the number of polypide.3 days adults are designated as the 0th day of life-span.Death standard is to touch nematode with wire, as polypide is motionless, is sentenced For death.Experimental result is shown in Table 1.
Influence of the table 1 according to the mysterious berry extract of the present invention to the nematode life-span
From table 1, after the mysterious berry extract prepared according to embodiment 1~3 is given to experiment nematode, treatment group The nematode life-span be obviously prolonged, illustrate that the mysterious berry extract for preparing of the present invention has the effect of good anti-aging, The extract prepared especially in accordance with embodiment 2, effect is significantly higher than positive drug (ganoderma lucidum).
Influence of the miracle fruit extract concentrations to the nematode life-span
Referring to Fig. 2, given the test agent, method described above processing nematode, processing are used as according to the mysterious berry extract of embodiment 2 Concentration is 0.001~0.01mg/mL, treatment group and each 10 wares of control group, ibid, the number of the polypide of record death daily.3 days Adult is designated as the 0th day of life-span.In tested concentration range, there is the effect in extension nematode life-span.And in 0.05mg/mL, The nematode lost of life, may be too high due to miracle fruit extract concentrations, more than caused by its lethasl concentration.
Mysterious berry extract delays nematode Aging mechanism to test
Nematode：Glp-1 (e2141) mutant nematode, buys from Shanghai model biotech inc.
Experiment material：The mysterious berry extract as made from embodiment 2；0.1%DMSO, Shanghai model biotechnology share has Limit company.
Experimental method：
Fluid nutrient medium life experiment：In each hole that wall scroll nematode is chosen to 96 orifice plates containing fluid nutrient medium, liquid Contain 12.5ug/mL (or 50uM) FUDR in body NGM culture mediums.Divide control group and treatment group, daily the number of the dead polypide of record Mesh.3 days adults are designated as the 0th day of life-span.Death is motionless after touch for wire.
Referring to Fig. 3, the miracle fruit extract-treated nematode with according to embodiments of the present invention 2 is represented, is extracted according to miracle fruit Influence experiment of the thing concentration to the nematode life-span, is selected with after the processing of 0.005mg/mL dosage, the nematode life-span with to photograph Than being not significantly different, show that mysterious berry extract delays the effect of nematode aging to be to rely on germ cell line signal path real Existing.
H2O2Stress tests
The hermaphroditic adult of 3 days will be synchronized with M9 wash buffers into 1.5mL EP pipes, then use M9 buffer solutions Wash 3 times, remove Escherichia coli OP50.By nematode distribution in the hole of 48 orifice plates, 3 holes of every group of nematode point, each hole is no less than 30 nematodes, H2O2Final concentration 0.5mM.Treatment group is respectively the mysterious berry extract obtained by prepared by embodiment 1~3, and Vc is the positive Control, dosage is 10ug/ml.Blank control group is supplied with M9 buffer solutions.Dead standard with wire to touch body Body is motionless to be defined.The experiment is repeated 2 times.Experimental result is shown in Fig. 4.
By Fig. 4's test result indicates that, blank control group is with H2O2Handle after 22 hours of nematode, the nematode of survival is very It is few, and with the nematode of miracle fruit extract-treated at 22 hours, processed nematode survival rate is above 50%.Thus, may be used Illustrate that treatment group is remarkably improved nematode to H2O2Tolerance, oxidative stress ability can be significantly increased.
Based on the studies above result, it was confirmed that there is anti-aging and enhancing oxygen according to the miracle fruit leaf extract of the present invention Change stress effect, available for prepare anti-aging or strengthen oxidative stress medicine, food or cosmetics.

Claims (10)

1. a kind of mysterious berry extract is in preparing for anti-aging or strengthening medicine, food or the cosmetics of oxidative stress Using, it is characterised in that the mysterious berry extract is extracted by following steps：
Negative pressure cavitation extraction is carried out to miracle fruit pulp with solution is extracted, extract solution is obtained；
After the obtained extract solution is concentrated, separated through macroporous absorbent resin, collect eluent；
The eluent through being concentrated under reduced pressure, is freeze-dried again, obtains mysterious berry extract；
The solution that extracts is the enzyme solutions that pH is 1.5~5.5, and the liquid-solid ratio for extracting solution and the miracle fruit pulp For 5~20mL/g.
2. application according to claim 1, wherein, the enzyme in the enzyme solutions is cellulase, pectase, or mass ratio The complex enzyme of the cellulase and pectase composition for 1: 2~6: 1.
3. application according to claim 1, wherein, the extraction organic acid for adjusting pH of solution.
4. application according to claim 1, wherein, the enzyme is 1.0~8.0mg/ with the miracle fruit pulp amount ratio g。
5. application according to claim 1, wherein, the Extracting temperature that the negative pressure cavitation is extracted is 20 DEG C~55 DEG C, is carried Pressure power is -0.04MPa~-0.07MPa.
6. application according to claim 1, wherein, the macroporous absorbent resin be selected from HP 2MGL, HP-20, ADS-5, ADS-8, ADS-17, D3520, D4020, D14, D16 type macroporous absorbent resin.
7. application according to claim 1, wherein, the eluent in the macroporous absorbent resin separation is 60~90% (v/v) ethanol water.
8. application according to claim 7, wherein, before being eluted with 60~90% (v/v) ethanol water First eluted with 10~20% (v/v) ethanol water.
9. application according to any one of claim 1 to 8, wherein, the mysterious berry extract relies on germ cell line Signal path realizes anti-aging or enhancing oxidative stress effect.
10. application according to any one of claim 1 to 8, wherein, the useful effect concentration of the mysterious berry extract For 0.001~0.01mg/mL.
CN201710322530.1A 2017-05-09 2017-05-09 The application of mysterious berry extract Pending CN107184620A (en)

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Publication number Priority date Publication date Assignee Title
CN107875403A (en) * 2017-10-31 2018-04-06 华南理工大学 A kind of anti-aging evaluation method based on model organism Caenorhabditis elegans and application
CN110772456A (en) * 2019-11-04 2020-02-11 中国热带农业科学院南亚热带作物研究所 Miracle fruit leaf compound and application thereof in anti-melanin formation product
CN111544603A (en) * 2020-05-11 2020-08-18 扬州大学 Function evaluation method of ginkgo fruit product

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CN103159727A (en) * 2011-12-16 2013-06-19 东北林业大学 Method for extracting and separating anthocyanidin and polysaccharide from blueberry pomace
CN102960734A (en) * 2012-11-20 2013-03-13 中国热带农业科学院南亚热带作物研究所 Method for extracting natural anti-oxidizing substance from miracle fruit

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107875403A (en) * 2017-10-31 2018-04-06 华南理工大学 A kind of anti-aging evaluation method based on model organism Caenorhabditis elegans and application
CN110772456A (en) * 2019-11-04 2020-02-11 中国热带农业科学院南亚热带作物研究所 Miracle fruit leaf compound and application thereof in anti-melanin formation product
CN110772456B (en) * 2019-11-04 2022-08-23 中国热带农业科学院南亚热带作物研究所 Miracle fruit leaf compound and application thereof in anti-melanin formation product
CN111544603A (en) * 2020-05-11 2020-08-18 扬州大学 Function evaluation method of ginkgo fruit product

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