CN107179411A - Multi-functional mass spectrum substrate target holder - Google Patents
Multi-functional mass spectrum substrate target holder Download PDFInfo
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- CN107179411A CN107179411A CN201710539749.7A CN201710539749A CN107179411A CN 107179411 A CN107179411 A CN 107179411A CN 201710539749 A CN201710539749 A CN 201710539749A CN 107179411 A CN107179411 A CN 107179411A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
- G01N33/6848—Methods of protein analysis involving mass spectrometry
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Abstract
The present invention provides a kind of multi-functional mass spectrum substrate target holder, it includes target holder main body and the gene substrate neck and albumen microorganism substrate neck that are arranged in the target holder main body, wherein described gene substrate neck and the albumen microorganism substrate neck are square structure, and are respectively arranged with neck through hole at four angles of the square structure.The flatness of wherein target holder neck directly affects Mass Spectrometer Method Mass accuracy, so as to influence the degree of accuracy, this target holder neck flatness ＜ 10um, Mass Spectrometer Method Mass accuracy ＜ 350ppm greatly improve the Mass Spectrometric Identification degree of accuracy.
Description
Technical field
The present invention relates to field of biological detection, more particularly to the multi-functional mass spectrum substrate target holder detected for BIOMARK.
Background technology
Flight time mass spectrum is started from the 1940s, being confined to electronic technology at that time and the backwardness of instrument and equipment, instrument
Resolution ratio is less than 100, it is difficult to popularization and application.Over time with the progress of science and technology, to the end of the eighties,
Hillenkamp etc. has been invented after substance assistant laser desorpted ionized new method, by this soft ionization method and flight time
Mass spectrum is combined, and forms a kind of new instrument：Substance assistant laser desorpted ionized/flight time mass spectrum, i.e. MALDI-TOF MS
(Matrix Assisted Laser Desorption Ionization Time of Flight Mass
Spectrometry), it is the new organic mass spectrometry of a kind of soft ionization developed in recent years, by introducing substrate molecule, is made
Testing molecule does not produce fragment, the problem of solving non-volatile and thermal instability large biological molecule desorption ionization, is point
One of important means of organic substance of the difficult volatilization of analysis.
MALDI-TOF MS principle：When the laser irradiating sample and substrate formed cocrystallization film with some strength,
Matrix absorbs generation electric charge transfer between energy, sample desorption, matrix-sample from laser and causes ionized sample molecule, electricity
From sample accelerate to fly over dirft tube under electric field action, it is different and be detected, root according to the flight time for reaching detector
The ratio between quality electric charge according to ion (M/Z) is directly proportional to detect different ions to the flight time of ion.
MALDI TOF have turned into recent years to be detected and identification polypeptide, protein, polysaccharide, nucleotides, glycoprotein, high polymer
And the powerful tool of a variety of synthetic polymers, and it is widely used in drug development, the scientific research of biotechnology and pharmacy corporation
Monitoring of the nuclear radiation of the bioanalysis in field and chemical detection and security department, chemical substance and bio-pathogen etc..
MALDI-TOF MS have sensitivity height, degree of accuracy height, high resolution, collection of illustrative plates simplicity, mass range wide and speed
The features such as fast, operationally sample preparation it is easy, can milligram ammonia, extensive, parallelization and increasingly automated processing biological sample to be checked,
And have special superiority determining large biological molecule and synthetic high polymer application aspect.Such as application MALDI-TOF MS are determined
The peptide mass fingerprinting of protein digestion, which is composed, cracks (PSD) fragment ion collection of illustrative plates and with reference to mass spectrum network data behind (PMF), source
Library searching, can obtain the sequence of polypeptide, protein.Genome SNP (SNPs) is carried out using MALDI-TOF
Analysis detection, can distinguish and differentiate relative molecular mass up to 7,000 or so (containing more than 20 bases), only exist 1 base difference
Different DNA.Especially it is worthy of note that, MALDI-TOF turn into life science proteome research in must be indispensable
One of important key technology.
MALDI-TOF can solve in current protein science following several potential challenges, the i.e. discovery of biomarker,
Molecule diagnosis research and development, protein high throughput analysis and proteomic map, but this is not limited to, MALDI-TOF MS are applied to
The discovery of biomarker and pathogenic mechanism is disclosed on molecular level, controlled so as to be applied to molecule diagnosis, target
Treat and personalized medicine, by more completely pointing out to grow for people, develop and the rule of vital movement such as metabolic regulation and tight
The genesis mechanism of weight disease, carries out the diagnosis preventing and treating of disease for the mankind and new drug development provides important theoretical foundation.
Traditional MALDI-TOF MS target plate application fields are single, the MALDI-TOF Axima that such as SHIMADZU is produced, target
Plate is served only for albumen microbial identification；The MALDI-TOF MASSARRY of AGENA productions, target plate is served only for genetic test；Do not have also
There are a kind of covering gene detection, the MALDI-TOF mass spectrum target plates of three kinds of applications of albumen microbial identification.
In addition, MALDI-TOF tradition target plate flux is fixed, albumen microorganism substrate flux has 384well, 48well, base
Because substrate flux has 384well, 96well, 24well, it is impossible to a variety of applications selection collocation, and can not multiple target plates enter simultaneously
Sample, multiple laboratory technicians can not be tested simultaneously, and Mass Spectrometer Method Mass accuracy is low, and the identification degree of accuracy is low.
The content of the invention
In order to solve the above-mentioned technical problem, an aspect of of the present present invention provides multi-functional mass spectrum substrate target holder, and it includes target holder
Main body and the gene substrate neck and albumen microorganism substrate neck being arranged in the target holder main body, wherein the gene substrate
Neck and the albumen microorganism substrate neck are square structure, and are respectively arranged with card at four angles of the square structure
Groove through hole.
Preferably, the target holder main body is rectangle, and is provided with one angle the direction lead angle as direction signs,
The lead angle number of degrees are 45 °.
Preferably, multi-functional mass spectrum substrate target holder is further provided with mass spectrum substrate neck, and along outside the target holder main body
The perpendicularly inward direction in surface, sets three steps, the depth of first step is away from the target holder in the mass spectrum substrate neck
The outer surface of main body is 0.5mm-1.5mm, and bottom of the depth away from the first step of second step is 1mm-2mm and the 3rd
Bottom of the depth of rank away from the second step is 0.3mm-1mm.
Preferably, the flatness ＜ 10um of the first step, Mass Spectrometer Method Mass accuracy ＜ 350ppm.
Preferably, wherein the second step is placed with the magnetic sheet of mass spectrum substrate back.
Preferably, wherein the 3rd step is placed with round sheet magnetic sheet, fixed absorption mass spectrum substrate.
Preferably, wherein the mass spectrum substrate neck is square, and card is respectively arranged with four square angles
Groove through hole.
Preferably, multi-functional mass spectrum substrate target holder is further provided with locating groove 2, and its width is 2mm-10mm, height
For 1mm-5mm, positioned for vacuum chamber sample introduction neck.
Preferably, wherein the quantity of the gene substrate neck is 4, the quantity of the albumen microorganism substrate neck is 2,
It can be used for the gene substrate and/or the albumen microorganism substrate of less than 2 of disposable detection less than 4 simultaneously.
Preferably, wherein the material of the target holder selects the group of free metal, silicon chip and graphene composition.
By the special warfare and superhigh precision of mass spectrum substrate target holder, MALDI-TOF Mass Spectrometer Method Mass accuracies are improved, from
And genetic test, the degree of accuracy of microprotein identification are improved, belong to Mass Spectrometer Method field.
The mass spectrum substrate target holder of the present invention is applied to the CLIN-TOF- that Beijing Yixin Bochuang Biotechnology Co., Ltd. produces
II clinic flight time mass spectrums.Gene substrate and albumen microorganism substrate can be placed simultaneously, and single injected sampling can be carried out simultaneously
Genetic test and albumen microbial identification, it is to avoid different detection difference sample introductions, save the time, single injected sampling can save 15 points
Clock, detects 5 times, saves 60 minutes time within one day, save 1320 minutes time within one month, a Spring Festival holidays save time 15840 points for one day
Clock.
In addition, the mass spectrum substrate target holder flux of the present invention is flexibly, a target holder can place gene substrate 4, the micro- life of albumen
Thing substrate 2, i.e. 4+2 patterns, can also 2+3,6+1 isotype, flux can set according to the detection demand of biological specimen；
Can many people operation of sample preparation simultaneously, a target holder is divided into exemplified by+2 albumen microorganism substrates of 4 gene substrates, altogether
It is divided into 6 unit substrates, sample can be prepared simultaneously with 6 laboratory technicians, printing operation, 6 units can be with single injected sampling.
The degree of accuracy is high, and the flatness of target holder neck directly affects Mass Spectrometer Method Mass accuracy, so that the degree of accuracy is influenceed, this target
Neck flatness ＜ 10um, Mass Spectrometer Method Mass accuracy ＜ 350ppm are held in the palm, the Mass Spectrometric Identification degree of accuracy is greatly improved.
Brief description of the drawings
Fig. 1 mass spectrum substrate target holder floor map.
Fig. 2 mass spectrum substrate target holder diagrammatic cross-sections.
Fig. 3 deafness standard items spectrograms.
Fig. 4 microorganism detection Escherichia coli spectrograms.
Fig. 5 genetic tests deafness plasmid spectrogram.
Embodiment
Now describe the various exemplary embodiment of the present invention in detail, the detailed description is not considered as the limit to the present invention
System, and it is understood as the more detailed description to certain aspects of the invention, characteristic and embodiment.
It should be understood that heretofore described term is only to be to describe special embodiment, this hair is not intended to limit
It is bright.In addition, for the number range in the present invention, it is thus understood that specifically disclose the scope upper and lower bound value and they
Between each median.Median and any other statement value in any statement value or stated ranges or in the model
The each less scope enclosed between interior median is also included in the present invention.These small range of upper and lower bounds can be only
On the spot include or exclude in the range of.
Unless otherwise stated, all technologies used herein and scientific terminology have the routine in field of the present invention
The identical meanings that technical staff is generally understood that.Although the present invention only describes preferred method and material, the present invention's
Implement or can also be used and similar or equivalent any method described herein and material in testing.
Embodiment one, target holder flatness influence on the qualification result degree of accuracy
By taking deaf gene standard items as an example, totally 18 mass spectra peaks, mass range 3000-9000Da, select three kinds of flatnesses
10 μm, 20 μm, 30 μm, every kind of same sample of flatness does 12 repetitions, analyzes the mass deviation at every peak of contrasting detection result,
Calculate mass accuracy.
Parameter setting：
Turing mode:linear
Mass Range:3000-9000
Max Laser Rep Rate:10.0
Power:90
Profiles:40
Shots:10
1) 10 μm of substrate slot bit plane degree ＜, 12 repetitions, each site peak deviation：(grey is maximum deviation)
Maximum deviation is less than 350ppm in 8214.4Da positions, deviation 2.78Da, mass accuracy.
2) 20 μm of substrate slot bit plane degree ＜, 12 repetitions, each site peak deviation：(grey is maximum deviation)
Maximum deviation is less than 500ppm in 8214.4Da positions, deviation 3.89Da, mass accuracy.
3) 30 μm of substrate slot bit plane degree ＜, 12 repetitions, each site peak deviation：(grey is maximum deviation)
Maximum deviation is less than 550ppm in 8214.4Da positions, deviation 4.26Da, mass accuracy.
See the deaf standard items spectrograms of Fig. 3 referring specifically to spectrogram.Target holder flatness is to spectrum it can be seen from Fig. 3 and upper form
Plot quality degree of accuracy influence is larger, and the index that the present invention reaches is：10 μm of target holder flatness ＜, mass accuracy ＜ 350ppm.
Embodiment two, microbial identification
5 kinds of fresh bacterial strains of microorganism are selected, every kind of 3 repetitions of bacterial strain, mass range 2000-20000Da, gathered data exists
BE3.0 softwares are given a mark, and check confidence level result.
Parameter setting：
Turing mode:linear
Mass Range:2000-20000
Max Laser Rep Rate:30.0
Power:60
Profiles:100
Shots:5
Spectrogram is shown in Fig. 4 microorganism detection Escherichia coli spectrograms.
Conclusion：25 points of confidence level ＞, is that qualification result is credible, and this 15 groups of data authentication result of experiment is correct, correctly
Rate 100%.
Embodiment three, genetic test
Deaf plasmid sample is selected, totally 20 heavy spectral peak, mass range 3000-9000Da, 5 repetitions of positive sample are cloudy
Property 5 repetitions of sample, analysis testing result identification the degree of accuracy.
Parameter setting：
Turing mode:linear
Mass Range:3000-9000
Max Laser Rep Rate:10.0
Power:90
Profiles:40
Shots:10
Deaf plasmid sample qualification result：
Spectrogram is shown in the deaf plasmid spectrogram of Fig. 5 genetic tests.
Conclusion：Based on 10 μm of target holders of flatness ＜, 10 deaf plasmid samples, 20 positions of each deafness plasmid are detected
Point, totally 200 sites, qualification result accuracy rate 100%.
General principle, principal character and the advantages of the present invention of the present invention has been shown and described above.The technology of the industry
Personnel are it should be appreciated that the present invention is not limited to the above embodiments, and the simply explanation described in above-described embodiment and specification is originally
The principle of invention, without departing from the spirit and scope of the present invention, various changes and modifications of the present invention are possible, the present invention
Claimed scope is by appended claims, specification and its equivalent thereof.
Claims (10)
1. a kind of multi-functional mass spectrum substrate target holder, it includes target holder main body and the gene substrate card being arranged in the target holder main body
Groove and albumen microorganism substrate neck, wherein the gene substrate neck and the albumen microorganism substrate neck are square knot
Structure, and it is respectively arranged with neck through hole at four angles of the square structure.
2. multi-functional mass spectrum substrate target holder according to claim 1, wherein the target holder main body is rectangle, and in one
The direction lead angle as direction signs is provided with angle, the lead angle number of degrees are 45 °.
3. multi-functional mass spectrum substrate target holder according to claim 1, it is further provided with mass spectrum substrate neck, and along institute
The perpendicularly inward direction of target holder body outer surface is stated, three steps, the depth of first step are set in the mass spectrum substrate neck
Outer surface of the degree away from the target holder main body is 0.5mm-1.5mm, and bottom of the depth away from the first step of second step is
Bottom of the depth away from the second step of 1mm-2mm and the 3rd step is 0.3mm-1mm.
4. multi-functional mass spectrum substrate target holder according to claim 3, wherein the flatness ＜ 10um of the first step, matter
Spectrum detection Mass accuracy ＜ 350ppm.
5. multi-functional mass spectrum substrate target holder according to claim 3, wherein the second step is placed with the mass spectrum substrate back of the body
The magnetic sheet in face.
6. multi-functional mass spectrum substrate target holder according to claim 3, wherein the 3rd step is placed with round sheet magnetic
Piece, fixed absorption mass spectrum substrate.
7. multi-functional mass spectrum substrate target holder according to claim 3, wherein the mass spectrum substrate neck is square, and in institute
State and be respectively arranged with neck through hole at four square angles.
8. multi-functional mass spectrum substrate target holder according to claim 1, it is further provided with locating groove, and its width is
2mm-10mm, is highly 1mm-5mm, for the positioning of vacuum chamber sample introduction neck.
9. multi-functional mass spectrum substrate target holder according to claim 1, wherein the quantity of the gene substrate neck is 4, institute
The quantity for stating albumen microorganism substrate neck is 2, and it can be used for the disposable gene substrate of detection less than 4 simultaneously and/or 2
Following albumen microorganism substrate.
10. multi-functional mass spectrum substrate target holder according to claim 1, wherein the material of the target holder selects free metal, silicon
The group of piece and graphene composition.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710539749.7A CN107179411B (en) | 2017-07-05 | 2017-07-05 | Multi-functional mass spectrum substrate target holder |
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| CN201710539749.7A CN107179411B (en) | 2017-07-05 | 2017-07-05 | Multi-functional mass spectrum substrate target holder |
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| CN107179411B CN107179411B (en) | 2019-01-29 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108148742A (en) * | 2018-01-03 | 2018-06-12 | 北京毅新博创生物科技有限公司 | A kind of mass spectrum substrate target holder for detection of biological samples |
| CN108267500A (en) * | 2018-01-03 | 2018-07-10 | 北京毅新博创生物科技有限公司 | Mass spectrum substrate target holder detects the purposes of biological sample for BIOMARK |
| CN108709927A (en) * | 2018-05-24 | 2018-10-26 | 华东理工大学 | It can disposal type mass spectrum target plate and its preparation method and application |
Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1999000657A1 (en) * | 1997-06-26 | 1999-01-07 | Perseptive Biosystems, Inc. | High density sample holder for analysis of biological samples |
| US20040163673A1 (en) * | 2003-01-15 | 2004-08-26 | Bruker Daltonik Gmbh | Method and device for cleaning desorption ion sources |
| US6825478B1 (en) * | 2003-10-10 | 2004-11-30 | Perseptive Biosystems, Inc. | MALDI plate with removable magnetic insert |
| US20070075241A1 (en) * | 2005-02-07 | 2007-04-05 | Yangsun Kim | Sample plate for MALDI mass spectrometry and process for manufacture of the same |
| CN102337341A (en) * | 2011-10-21 | 2012-02-01 | 马庆伟 | MALDI-TOF (Matrix-assisted laser desorption/ionization-Time-of-flight) mass spectrum double internal standard and quantitative detecting method thereof |
| CN204613150U (en) * | 2015-03-13 | 2015-09-02 | 北京毅新博创生物科技有限公司 | The hydrophobicity of Mass Spectrometer Method concentrates target plate |
| US9214323B1 (en) * | 2014-09-02 | 2015-12-15 | Virgin Instruments Corporation | Method and apparatus for transporting sample plates between chambers of a mass spectrometer |
| CN105206490A (en) * | 2014-06-19 | 2015-12-30 | 西安福安创意咨询有限责任公司 | Multiple-combination sample target frame |
| CN105489466A (en) * | 2014-10-06 | 2016-04-13 | 布鲁克道尔顿有限公司 | MALDI support with magnetically held spring steel plate |
| CN106198709A (en) * | 2016-08-30 | 2016-12-07 | 中山大学 | Sample tray in universal laser ablation plasma mass spectrograph sample cell |
| CN205981818U (en) * | 2016-07-08 | 2017-02-22 | 浙江大学 | A MALDI target plate that is used for sample to fix in detection of chip carrier normal position |
-
2017
- 2017-07-05 CN CN201710539749.7A patent/CN107179411B/en active Active
Patent Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1999000657A1 (en) * | 1997-06-26 | 1999-01-07 | Perseptive Biosystems, Inc. | High density sample holder for analysis of biological samples |
| US20040163673A1 (en) * | 2003-01-15 | 2004-08-26 | Bruker Daltonik Gmbh | Method and device for cleaning desorption ion sources |
| US6825478B1 (en) * | 2003-10-10 | 2004-11-30 | Perseptive Biosystems, Inc. | MALDI plate with removable magnetic insert |
| US20070075241A1 (en) * | 2005-02-07 | 2007-04-05 | Yangsun Kim | Sample plate for MALDI mass spectrometry and process for manufacture of the same |
| CN102337341A (en) * | 2011-10-21 | 2012-02-01 | 马庆伟 | MALDI-TOF (Matrix-assisted laser desorption/ionization-Time-of-flight) mass spectrum double internal standard and quantitative detecting method thereof |
| CN105206490A (en) * | 2014-06-19 | 2015-12-30 | 西安福安创意咨询有限责任公司 | Multiple-combination sample target frame |
| US9214323B1 (en) * | 2014-09-02 | 2015-12-15 | Virgin Instruments Corporation | Method and apparatus for transporting sample plates between chambers of a mass spectrometer |
| CN105489466A (en) * | 2014-10-06 | 2016-04-13 | 布鲁克道尔顿有限公司 | MALDI support with magnetically held spring steel plate |
| CN204613150U (en) * | 2015-03-13 | 2015-09-02 | 北京毅新博创生物科技有限公司 | The hydrophobicity of Mass Spectrometer Method concentrates target plate |
| CN205981818U (en) * | 2016-07-08 | 2017-02-22 | 浙江大学 | A MALDI target plate that is used for sample to fix in detection of chip carrier normal position |
| CN106198709A (en) * | 2016-08-30 | 2016-12-07 | 中山大学 | Sample tray in universal laser ablation plasma mass spectrograph sample cell |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108148742A (en) * | 2018-01-03 | 2018-06-12 | 北京毅新博创生物科技有限公司 | A kind of mass spectrum substrate target holder for detection of biological samples |
| CN108267500A (en) * | 2018-01-03 | 2018-07-10 | 北京毅新博创生物科技有限公司 | Mass spectrum substrate target holder detects the purposes of biological sample for BIOMARK |
| CN108709927A (en) * | 2018-05-24 | 2018-10-26 | 华东理工大学 | It can disposal type mass spectrum target plate and its preparation method and application |
| CN108709927B (en) * | 2018-05-24 | 2021-09-07 | 华东理工大学 | Disposable mass spectrum target plate and preparation method and application thereof |
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|---|---|
| CN107179411B (en) | 2019-01-29 |
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