CN107177477A - A kind of sealed sample processing apparatus - Google Patents
A kind of sealed sample processing apparatus Download PDFInfo
- Publication number
- CN107177477A CN107177477A CN201710465668.7A CN201710465668A CN107177477A CN 107177477 A CN107177477 A CN 107177477A CN 201710465668 A CN201710465668 A CN 201710465668A CN 107177477 A CN107177477 A CN 107177477A
- Authority
- CN
- China
- Prior art keywords
- reaction tank
- processing apparatus
- reaction
- sample processing
- waste liquid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
Abstract
The invention discloses a kind of sealed sample processing apparatus, it includes reaction unit and the fixing device for fixing the reaction unit；The fixing device is arranged on the outer wall of the reaction unit；The reaction unit includes reaction tank and the waste liquid pool below reaction tank；Control valve is provided between the reaction tank and the waste liquid pool, and passes through connection/barrier of control valve control reaction tank and waste liquid pool.Its simple in construction, compact of the processing unit that the present invention is provided, it is easy to use, only coordinate simple external accessory to complete the nucleic acid extraction of sample, can be widely applied to major laboratories and primary care.
Description
Technical field
The present invention relates to biological technical field, more particularly to a kind of sealed sample processing apparatus.
Background technology
At present, usually needed in biological technical field from various biological tissues or cell extract genomic DNA or
Person RNA, and determine its composition using nucleic acid amplification detection technique.During existing detection of nucleic acids in laboratory conditions, made
Template is all that, by independent sample extraction process, and extraction environment is all in the environment of non-close so that extracted
During easily cause template cross pollution, then by amplification, the false positive signal produced by product pollution can cause testing result
Misinterpretation.
In order to solve the above problems, now in the presence of using realized in the system of closing extraction to determined nucleic acid,
The method of amplification and detection.But, the construction of existing provision for sealing is complicated, expensive, and the control of periphery is also excessively numerous
Trivial complexity.
Thus prior art could be improved and improve.
The content of the invention
The technical problem to be solved in the present invention is that there is provided a kind of sealed sample process in view of the shortcomings of the prior art
Device, with solve it is existing it is closed adjust extraction element complicated and high cost the problem of.
In order to solve the above-mentioned technical problem, the technical solution adopted in the present invention is as follows：
A kind of sealed sample processing apparatus, it includes：Reaction unit and the fixing device for fixing the reaction unit；
The fixing device is arranged on the outer wall of the reaction unit；The reaction unit includes reaction tank and under reaction tank
The waste liquid pool of side；Control valve is provided between the reaction tank and the waste liquid pool, and reaction tank is controlled by the control valve
With connection/barrier of waste liquid pool.
The sealed sample processing apparatus, wherein, the fixing device includes connecting plate and is built in the connection
Conduction pipe in plate for air pressure in balanced reaction pond, the connecting plate is structure as a whole with the reaction unit.
The sealed sample processing apparatus, wherein, the connecting plate includes the first connection end, the second connection end and position
Fixed part between first connection end and the second connection end；First connection end is arranged at the outer wall of the reaction tank
On, second connection end is arranged on the outer wall of the waste liquid pool.
The sealed sample processing apparatus, wherein, the fixed part has interval with the reaction unit, to cause
Stating fixed part can be clamped.
The sealed sample processing apparatus, wherein, the connecting plate is handle in a rectangular trapezoid, and the handle is set
In on the reaction unit, to fix the reaction unit by clamping the handle.
The sealed sample processing apparatus, wherein, the thickness of the handle is 3-5mm.
The sealed sample processing apparatus, wherein, described conduction pipe one end connected reaction pond, other end connection waste liquid
Pond；When being reacted in reaction tank, the conduction pipe is by the gas communication in reaction tank to waste liquid pool, to balance the reaction
Air pressure in pond.
The sealed sample processing apparatus, wherein, the reaction tank includes reacting part and under the reacting part
The first connecting portion of side, the one end of the reacting part away from first connecting portion is provided with upper port, the upper port and is sealed with
Diaphragm seal；The first connecting portion is connected with the control valve.
The sealed sample processing apparatus, wherein, the waste liquid pool includes containing liquid portion and in the Sheng liquid portion
The second connecting portion of side, the second connecting portion is connected with the control valve.
The sealed sample processing apparatus, wherein, the first taper port is provided with above the second connecting portion, it is described
Be provided with the second taper port below first connecting portion, the first taper port and the second taper port respectively with the control
Valve is connected, to control both connection/barriers by the control valve.
Beneficial effect：Compared with prior art, the invention provides reaction unit and for fixing the reaction unit
Fixing device；The fixing device is arranged on the outer wall of the reaction unit；The reaction unit include reaction tank and
Waste liquid pool below reaction tank；Control valve is provided between the reaction tank and the waste liquid pool, and passes through the control
Valve controls connection/barrier of reaction tank and waste liquid pool.Its simple in construction, compact of the processing unit that the present invention is provided, is used
It is convenient, only coordinate simple external accessory to complete the nucleic acid extraction of sample, can be widely applied to major laboratories and
Primary care.
Brief description of the drawings
The structural representation for the sealed sample processing apparatus that Fig. 1 provides for the present invention.
First visual angle schematic diagram of the sealed sample processing apparatus that Fig. 2 provides for the present invention.
Second visual angle schematic diagram of the sealed sample processing apparatus that Fig. 3 provides for the present invention.
3rd visual angle schematic diagram of the sealed sample processing apparatus that Fig. 4 provides for the present invention.
The schematic diagram of first use state of the sealed sample processing apparatus that Fig. 5 provides for the present invention.
The schematic diagram of second use state of the sealed sample processing apparatus that Fig. 6 provides for the present invention.
Embodiment
The present invention provides a kind of sealed sample processing apparatus, to make the purpose of the present invention, technical scheme and effect more
Clear, clear and definite, the present invention is described in more detail for the embodiment that develops simultaneously referring to the drawings.It should be appreciated that described herein
Specific embodiment only to explain the present invention, is not intended to limit the present invention.
Below in conjunction with the accompanying drawings, by the description to embodiment, the content of the invention is described further.
A kind of sealed sample processing apparatus is present embodiments provided, as Figure 1-4, the processing unit includes fixing
Device and reaction unit；The fixing device is arranged on the outer wall of the reaction unit, and control is provided with the reaction unit
The reaction unit is isolated into reaction tank 3 and the waste liquid pool 4 positioned at the lower section of reaction tank 3 by valve 5 processed, the control valve 5.
The waste liquid pool 4 and the reaction tank 3 are respectively provided with individual cavity, and two individual cavities are controlled by the control valve 5
Connection/barrier, i.e., when the control valve 3 is in opening, the reaction tank 3 is connected with the waste liquid pool 4；When the control
When valve 3 processed is closed, the reaction tank 3 obstructs into two individual cavities with the waste liquid pool 4.What the present embodiment was provided
In sealed sample processing apparatus, the reaction tank has individual cavity, when carrying out sample process by sample needle by sample
Or reaction reagent is added sequentially to react in reaction tank, and apply the condition such as Magneto separate and heating in reaction tank side so that anti-
It should be carried out in closed reaction tank, and control waste liquid to be flowed into from reaction tank in waste liquid pool by control valve, realize sample
Processing is carried out in confined conditions.Meanwhile, the processing unit is simple in construction, and production cost is low.
As Figure 1-4, the fixing device includes connecting plate 2 and conduction pipe 6, and the conduction pipe 6 is built in the company
In fishplate bar 2, and connect the reaction tank 3 and the pond of the waste liquid 4 so that the gas in the reaction tank 3 can be by described
Conduction pipe 6 enters the waste liquid pool 4, can so balance the air pressure in the reaction tank 3, it is to avoid due to being produced in course of reaction
Gas and cause air pressure in reaction tank excessive, and influence the life-span of reaction tank.In the present embodiment, the conduction pipe 6 is two ends
Round tube with opening, the opening of described round tube one end is connected with the reaction tank 3, opening and the waste liquid pool 4 of the other end
Connection, to form air pressure passageway between the reaction tank 3 and waste liquid pool 4.
As Figure 1-4, the connecting plate 2 includes the first connection end 21, the second connection end 22 and fixed part 23, described the
One connection end 21, the second connection end 22 and fixed part 23 are structure as a whole, and the fixed part 23 is located at the described first connection
Between the connection end 22 of end 21 and second.First connection end 21 is connected to the outer wall of the reaction tank 3, the second connecting portion
22 are connected to the outer wall of the waste liquid pool 4, and the connection end 22 of first connection end 21 and second is located at the reaction tank
The same side.For example, the first connection end 21 is located at the left side of the reaction tank 3, accordingly, second connection end 22 also is located at institute
State the left side of waste liquid pool 4.Certainly, the connection end 22 of the first connection end 21 and second also can be located at the right side of the reaction tank 3
Side etc..
Further, bearing of trend of the fixed part 23 along the reaction tank 3 to waste liquid pool 4 is set, and the fixation
There is gap between the connecting portion of portion 23 and the reaction tank 3 and waste liquid pool 4, can so be clamped in order to external equipment described solid
Determine portion 23.In the present embodiment, the connecting plate 2 is the grip structure of right-angled trapezium, and the handle is arranged at the reaction dress
Put, user can pass through the hand-held handle and the fixed reaction unit, it is to avoid reaction unit shakes during the course of the reaction
The problem of moving, fall.In actual applications, the thickness of the connecting plate 2 is preferably 3-5mm, in order to which user grips.
As shown in figures 1 to 6, the reaction tank 3 include reacting part 31 and first connecting portion 32, the reacting part 31 with it is described
First connecting portion 32 is structure as a whole, and the first connecting portion 32 is located at the lower end of the reacting part 31.The reacting part
31 top is provided with upper port, and the first connecting portion 32 is connected with the control valve 5；It is sealed with the upper port close
Sealer 1, and the diaphragm seal 1, reacting part 31, first connecting portion 32 and control valve 5 form the separate chamber of the reaction tank 3
Body so that sample can react in the individual cavity.In actual applications, the diaphragm seal can be solid with the reacting part
Connect or be detachably connected calmly.
As shown in figures 1 to 6, the waste liquid pool 4 includes containing liquid portion 41 and second connecting portion 42, the second connecting portion 42
Positioned at the top for containing liquid portion 41 and positioned at the lower section of first connecting portion 32, the Sheng liquid portion 41 and the second connecting portion 42
It is structure as a whole, the second connecting portion 42 is connected with the control valve 5, and itself and first is controlled by the control valve 5
Connection/barrier of connecting portion 32.When the control valve 5 control second connecting portion 42 is obstructed with first connecting portion 32, the control
Valve 5 processed, second connecting portion 42 and Sheng liquid portion 41 constitute the individual cavity of the waste liquid pool 4.It is worth explanation, when the control
When valve 5 obstructs the second connecting portion 42 and first connecting portion 32, the individual cavity of the reaction tank 3 and the waste liquid pool 4
Individual cavity is only connected by conduction pipe 6, carries out the circulation of air pressure.
Further, the reacting part 31 and Sheng liquid portion 41 use cylindrical structure, the first connecting portion 32 and second
Connecting portion 42 uses pyramid type structure.The top of second connecting portion 42 is provided with the first taper port, the first connecting portion
32 lower sections are provided with the second taper port, and the first taper port and the second taper port are connected with the control valve 5 respectively
Connect, to control both connection/barriers by the control valve 5.In the variant embodiment of this implementation, the reaction tank 3 with
The waste liquid pool 4 can use integrative-structure, and the reaction tank 3 is provided directly with connecting portion, the connection with the waste liquid pool 4
Control valve 5 is provided with portion, to control connection/barrier of the reaction tank and waste liquid pool.In actual applications, the reaction dress
Put and fixing device can be using materials such as polypropylene PP.
It can be used in the reaction that paramagnetic particle method extracts nucleic acid the sealed sample processing apparatus that the present embodiment is provided, described
The specific work process of processing unit can be：The fixing device is clamped by external clamping devices, is filled with the fixed processing
Put, then the control valve is set to off closed state；Pierce through diaphragm seal 1 sample is injected into reaction tank 3 by sample needle 7
In, then draw lysate and be injected into reaction tank 3, sample needle 7, which is extend into reaction tank 3, mixes sample and lysate piping and druming
Afterwards；Apply heater in the side wall of reaction tank 3（Such as, heating film）Ten are incubated to carry out 60 DEG C to the mixed liquor in reaction tank 3
Minute, the air pressure in heating process in reaction tank 3 can be connected with waste liquid pool by conduction pipe 6 and reach balance.Completed in cracking
Draw magnetic bead by sample needle 7 afterwards and combine liquid and inject in reaction tank 3, after fully being mixed with lysate, in the side wall of reaction tank 3
Apply magnetic patch to carry out Magneto separate to the liquid in reaction tank 3 so that magnetic bead is adsorbed on the side wall of reaction tank 3.Open control
Valve processed causes reaction tank 3 to be connected with waste liquid pool 4（As shown in Figure 5）, the switching channels that the liquid in reaction tank 3 passes through control valve 5
It is flowed into waste liquid pool, and the magnetic bead for being adsorbed with nucleic acid then stays in the side wall of reaction tank 3.Liquid in question response pond 3 all flows
After entering into waste liquid pool 4, the switching channels cut-off reaction tank 3 and waste liquid pool 4 of closing control valve（As shown in Figure 6）Complete nucleic acid with
Magnetic bead is combined；Cleaning fluid is added in reaction tank 3 by sample needle 7, above-mentioned piping and druming mixing, Magneto separate is repeated and abandons waste liquid extremely
Operation in waste liquid pool 3, completes magnetic bead cleaning；Finally eluent is added in reaction tank 3 by sample needle 7, it is then outside
Apply side wall of the temperature conditionss in reaction tank 3, Magneto separate, valve after being incubated ten minutes to 65 DEG C of mixed liquor progress in reaction tank 3
Door 5 remains turned-off the state of cut-off, and the clear liquid in reaction tank 3 is suctioned out and moved in the container sterilized with sample needle 7, is used for
Further detection.
Finally it should be noted that：The above embodiments are merely illustrative of the technical solutions of the present invention, rather than its limitations；Although
The present invention is described in detail with reference to the foregoing embodiments, it will be understood by those within the art that：It still may be used
To be modified to the technical scheme described in foregoing embodiments, or equivalent substitution is carried out to which part technical characteristic；
And these modification or replace, do not make appropriate technical solution essence depart from various embodiments of the present invention technical scheme spirit and
Scope.
Claims (10)
1. a kind of sealed sample processing apparatus, it is characterised in that it includes：Reaction unit and for fix it is described reaction dress
The fixing device put；The fixing device is arranged on the outer wall of the reaction unit；The reaction unit include reaction tank with
And the waste liquid pool below reaction tank；Control valve is provided between the reaction tank and the waste liquid pool, and passes through the control
Connection/barrier of valve control reaction tank and waste liquid pool processed.
2. sealed sample processing apparatus according to claim 1, it is characterised in that the fixing device include connecting plate with
And the conduction pipe in the connecting plate for air pressure in balanced reaction pond is built in, the connecting plate is one with the reaction unit
Body structure.
3. sealed sample processing apparatus according to claim 2, it is characterised in that the connecting plate includes the first connection
End, the second connection end and the fixed part between first connection end and the second connection end；First connection end is set
On the outer wall for being placed in the reaction tank, second connection end is arranged on the outer wall of the waste liquid pool.
4. sealed sample processing apparatus according to claim 3, it is characterised in that the fixed part and the reaction unit
With interval, to cause the fixed part to be clamped.
5. according to any sealed sample processing apparatus of claim 2-4, it is characterised in that the connecting plate is rectangular
Trapezoidal handle, the handle is arranged on the reaction unit, to fix the reaction by clamping the handle
Device.
6. sealed sample processing apparatus according to claim 5, it is characterised in that the thickness of the handle is 3-5mm.
7. sealed sample processing apparatus according to claim 2, it is characterised in that conduction pipe one end connection reaction
Pond, other end connection waste liquid pool；When being reacted in reaction tank, the conduction pipe is by the gas communication in reaction tank to waste liquid
Pond, to balance the air pressure in the reaction tank.
8. sealed sample processing apparatus according to claim 1, it is characterised in that the reaction tank include reacting part and
First connecting portion below the reacting part, the one end of the reacting part away from first connecting portion is provided with upper port, institute
State and diaphragm seal is sealed with upper port；The first connecting portion is connected with the control valve.
9. sealed sample processing apparatus according to claim 8, it is characterised in that the waste liquid pool include containing liquid portion and
Second connecting portion above the Sheng liquid portion, the second connecting portion is connected with the control valve.
10. sealed sample processing apparatus according to claim 9, it is characterised in that set above the second connecting portion
Have and the second taper port is provided with below the first taper port, the first connecting portion, the first taper port and the second cone
Shape port is connected with the control valve respectively, to control both connection/barriers by the control valve.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710465668.7A CN107177477A (en) | 2017-06-19 | 2017-06-19 | A kind of sealed sample processing apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710465668.7A CN107177477A (en) | 2017-06-19 | 2017-06-19 | A kind of sealed sample processing apparatus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107177477A true CN107177477A (en) | 2017-09-19 |
Family
ID=59835404
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710465668.7A Pending CN107177477A (en) | 2017-06-19 | 2017-06-19 | A kind of sealed sample processing apparatus |
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| Country | Link |
|---|---|
| CN (1) | CN107177477A (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1995958A (en) * | 2006-11-13 | 2007-07-11 | 李治国 | Bioactive substance automatic extracting device |
| CN202408161U (en) * | 2011-12-31 | 2012-09-05 | 蔡芳华 | Hourglass type tea maker |
| CN203538970U (en) * | 2013-11-05 | 2014-04-16 | 雷进斌 | Hourglass type filtering teapot |
| CN104399411A (en) * | 2014-11-12 | 2015-03-11 | 陕西师范大学 | Gas velocity adjustable solid-liquid reactive gas generator and method thereof |
-
2017
- 2017-06-19 CN CN201710465668.7A patent/CN107177477A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1995958A (en) * | 2006-11-13 | 2007-07-11 | 李治国 | Bioactive substance automatic extracting device |
| CN202408161U (en) * | 2011-12-31 | 2012-09-05 | 蔡芳华 | Hourglass type tea maker |
| CN203538970U (en) * | 2013-11-05 | 2014-04-16 | 雷进斌 | Hourglass type filtering teapot |
| CN104399411A (en) * | 2014-11-12 | 2015-03-11 | 陕西师范大学 | Gas velocity adjustable solid-liquid reactive gas generator and method thereof |
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