CN107174605A - A kind of proso millet prolamin n-butanol extract and its preparation method and application - Google Patents
A kind of proso millet prolamin n-butanol extract and its preparation method and application Download PDFInfo
- Publication number
- CN107174605A CN107174605A CN201710357078.2A CN201710357078A CN107174605A CN 107174605 A CN107174605 A CN 107174605A CN 201710357078 A CN201710357078 A CN 201710357078A CN 107174605 A CN107174605 A CN 107174605A
- Authority
- CN
- China
- Prior art keywords
- proso millet
- millet prolamin
- prolamin
- butanol extract
- extract
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 235000007199 Panicum miliaceum Nutrition 0.000 title claims abstract description 103
- 108060006613 prolamin Proteins 0.000 title claims abstract description 103
- 239000002021 butanolic extract Substances 0.000 title claims abstract description 67
- 238000002360 preparation method Methods 0.000 title claims description 9
- 244000022203 blackseeded proso millet Species 0.000 title 1
- 240000008114 Panicum miliaceum Species 0.000 claims abstract description 102
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 48
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 claims abstract description 33
- 239000000284 extract Substances 0.000 claims abstract description 23
- 206010012601 diabetes mellitus Diseases 0.000 claims abstract description 20
- 238000000034 method Methods 0.000 claims abstract description 18
- 235000013305 food Nutrition 0.000 claims abstract description 15
- 239000003814 drug Substances 0.000 claims abstract description 14
- 238000000605 extraction Methods 0.000 claims abstract description 10
- 208000016354 hearing loss disease Diseases 0.000 claims abstract description 9
- 208000024891 symptom Diseases 0.000 claims abstract description 7
- 230000002218 hypoglycaemic effect Effects 0.000 claims abstract description 4
- 239000007788 liquid Substances 0.000 claims description 18
- 238000001704 evaporation Methods 0.000 claims description 16
- 230000008020 evaporation Effects 0.000 claims description 15
- 239000000047 product Substances 0.000 claims description 10
- 230000036541 health Effects 0.000 claims description 6
- 239000006228 supernatant Substances 0.000 claims description 3
- 230000006872 improvement Effects 0.000 claims description 2
- 239000012675 alcoholic extract Substances 0.000 claims 1
- 241000207929 Scutellaria Species 0.000 abstract description 20
- 150000002338 glycosides Chemical group 0.000 abstract description 20
- 229930003944 flavone Natural products 0.000 abstract description 19
- 235000011949 flavones Nutrition 0.000 abstract description 19
- GAMYVSCDDLXAQW-AOIWZFSPSA-N Thermopsosid Natural products O(C)c1c(O)ccc(C=2Oc3c(c(O)cc(O[C@H]4[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O4)c3)C(=O)C=2)c1 GAMYVSCDDLXAQW-AOIWZFSPSA-N 0.000 abstract description 10
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 abstract description 10
- -1 flavone compound Chemical class 0.000 abstract description 9
- 239000008103 glucose Substances 0.000 abstract description 9
- 239000004480 active ingredient Substances 0.000 abstract description 5
- 238000000926 separation method Methods 0.000 abstract description 4
- 208000004880 Polyuria Diseases 0.000 abstract description 3
- 230000035619 diuresis Effects 0.000 abstract description 3
- 238000005516 engineering process Methods 0.000 abstract description 3
- 239000000470 constituent Substances 0.000 abstract description 2
- 201000001421 hyperglycemia Diseases 0.000 abstract description 2
- 239000002994 raw material Substances 0.000 abstract description 2
- 238000013459 approach Methods 0.000 abstract 1
- 150000001875 compounds Chemical group 0.000 abstract 1
- 230000004580 weight loss Effects 0.000 abstract 1
- 241000699666 Mus <mouse, genus> Species 0.000 description 18
- 229930182470 glycoside Natural products 0.000 description 16
- 239000000843 powder Substances 0.000 description 16
- ZSJLQEPLLKMAKR-GKHCUFPYSA-N streptozocin Chemical compound O=NN(C)C(=O)N[C@H]1[C@@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O ZSJLQEPLLKMAKR-GKHCUFPYSA-N 0.000 description 11
- ZSJLQEPLLKMAKR-UHFFFAOYSA-N Streptozotocin Natural products O=NN(C)C(=O)NC1C(O)OC(CO)C(O)C1O ZSJLQEPLLKMAKR-UHFFFAOYSA-N 0.000 description 10
- 239000008280 blood Substances 0.000 description 10
- 210000004369 blood Anatomy 0.000 description 10
- 230000000694 effects Effects 0.000 description 10
- 229960001052 streptozocin Drugs 0.000 description 10
- 150000002213 flavones Chemical class 0.000 description 9
- 241000220223 Fragaria Species 0.000 description 8
- 241000699670 Mus sp. Species 0.000 description 8
- 238000003304 gavage Methods 0.000 description 8
- 239000012071 phase Substances 0.000 description 8
- 235000016623 Fragaria vesca Nutrition 0.000 description 7
- 235000011363 Fragaria x ananassa Nutrition 0.000 description 7
- 238000006243 chemical reaction Methods 0.000 description 7
- JMGZEFIQIZZSBH-UHFFFAOYSA-N Bioquercetin Natural products CC1OC(OCC(O)C2OC(OC3=C(Oc4cc(O)cc(O)c4C3=O)c5ccc(O)c(O)c5)C(O)C2O)C(O)C(O)C1O JMGZEFIQIZZSBH-UHFFFAOYSA-N 0.000 description 6
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 6
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 6
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- 230000037396 body weight Effects 0.000 description 6
- IVTMALDHFAHOGL-UHFFFAOYSA-N eriodictyol 7-O-rutinoside Natural products OC1C(O)C(O)C(C)OC1OCC1C(O)C(O)C(O)C(OC=2C=C3C(C(C(O)=C(O3)C=3C=C(O)C(O)=CC=3)=O)=C(O)C=2)O1 IVTMALDHFAHOGL-UHFFFAOYSA-N 0.000 description 6
- FDRQPMVGJOQVTL-UHFFFAOYSA-N quercetin rutinoside Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC=2C(C3=C(O)C=C(O)C=C3OC=2C=2C=C(O)C(O)=CC=2)=O)O1 FDRQPMVGJOQVTL-UHFFFAOYSA-N 0.000 description 6
- 230000009467 reduction Effects 0.000 description 6
- 235000005493 rutin Nutrition 0.000 description 6
- IKGXIBQEEMLURG-BKUODXTLSA-N rutin Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](C)O[C@@H]1OC[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](OC=2C(C3=C(O)C=C(O)C=C3OC=2C=2C=C(O)C(O)=CC=2)=O)O1 IKGXIBQEEMLURG-BKUODXTLSA-N 0.000 description 6
- ALABRVAAKCSLSC-UHFFFAOYSA-N rutin Natural products CC1OC(OCC2OC(O)C(O)C(O)C2O)C(O)C(O)C1OC3=C(Oc4cc(O)cc(O)c4C3=O)c5ccc(O)c(O)c5 ALABRVAAKCSLSC-UHFFFAOYSA-N 0.000 description 6
- 229960004555 rutoside Drugs 0.000 description 6
- 239000000243 solution Substances 0.000 description 6
- 241000894007 species Species 0.000 description 6
- 229930003935 flavonoid Natural products 0.000 description 5
- 150000002215 flavonoids Chemical class 0.000 description 5
- 235000017173 flavonoids Nutrition 0.000 description 5
- 238000004809 thin layer chromatography Methods 0.000 description 5
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 4
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 4
- REFJWTPEDVJJIY-UHFFFAOYSA-N Quercetin Chemical compound C=1C(O)=CC(O)=C(C(C=2O)=O)C=1OC=2C1=CC=C(O)C(O)=C1 REFJWTPEDVJJIY-UHFFFAOYSA-N 0.000 description 4
- VSCWAEJMTAWNJL-UHFFFAOYSA-K aluminium trichloride Chemical compound Cl[Al](Cl)Cl VSCWAEJMTAWNJL-UHFFFAOYSA-K 0.000 description 4
- 235000020188 drinking water Nutrition 0.000 description 4
- 239000003651 drinking water Substances 0.000 description 4
- 229940079593 drug Drugs 0.000 description 4
- 238000004128 high performance liquid chromatography Methods 0.000 description 4
- 238000011534 incubation Methods 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- LPXPTNMVRIOKMN-UHFFFAOYSA-M sodium nitrite Chemical compound [Na+].[O-]N=O LPXPTNMVRIOKMN-UHFFFAOYSA-M 0.000 description 4
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical group CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 description 3
- 238000002835 absorbance Methods 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 3
- 235000021028 berry Nutrition 0.000 description 3
- 238000001514 detection method Methods 0.000 description 3
- 230000000116 mitigating effect Effects 0.000 description 3
- 235000005875 quercetin Nutrition 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 235000000346 sugar Nutrition 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 2
- RXKMOPXNWTYEHI-RDRKJGRWSA-N Flunarizine hydrochloride Chemical group Cl.Cl.C1=CC(F)=CC=C1C(C=1C=CC(F)=CC=1)N1CCN(C\C=C\C=2C=CC=CC=2)CC1 RXKMOPXNWTYEHI-RDRKJGRWSA-N 0.000 description 2
- ZVOLCUVKHLEPEV-UHFFFAOYSA-N Quercetagetin Natural products C1=C(O)C(O)=CC=C1C1=C(O)C(=O)C2=C(O)C(O)=C(O)C=C2O1 ZVOLCUVKHLEPEV-UHFFFAOYSA-N 0.000 description 2
- HWTZYBCRDDUBJY-UHFFFAOYSA-N Rhynchosin Natural products C1=C(O)C(O)=CC=C1C1=C(O)C(=O)C2=CC(O)=C(O)C=C2O1 HWTZYBCRDDUBJY-UHFFFAOYSA-N 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
- 229960000583 acetic acid Drugs 0.000 description 2
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 2
- 235000011114 ammonium hydroxide Nutrition 0.000 description 2
- 238000009835 boiling Methods 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 239000000287 crude extract Substances 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 238000006073 displacement reaction Methods 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 150000002212 flavone derivatives Chemical class 0.000 description 2
- 239000012362 glacial acetic acid Substances 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- MWDZOUNAPSSOEL-UHFFFAOYSA-N kaempferol Natural products OC1=C(C(=O)c2cc(O)cc(O)c2O1)c3ccc(O)cc3 MWDZOUNAPSSOEL-UHFFFAOYSA-N 0.000 description 2
- 210000004185 liver Anatomy 0.000 description 2
- 230000014759 maintenance of location Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000012982 microporous membrane Substances 0.000 description 2
- VLTRZXGMWDSKGL-UHFFFAOYSA-N perchloric acid Chemical compound OCl(=O)(=O)=O VLTRZXGMWDSKGL-UHFFFAOYSA-N 0.000 description 2
- 229960001285 quercetin Drugs 0.000 description 2
- 239000013558 reference substance Substances 0.000 description 2
- 239000011265 semifinished product Substances 0.000 description 2
- 239000000741 silica gel Substances 0.000 description 2
- 229910002027 silica gel Inorganic materials 0.000 description 2
- 235000010288 sodium nitrite Nutrition 0.000 description 2
- WCGUUGGRBIKTOS-GPOJBZKASA-N (3beta)-3-hydroxyurs-12-en-28-oic acid Chemical compound C1C[C@H](O)C(C)(C)[C@@H]2CC[C@@]3(C)[C@]4(C)CC[C@@]5(C(O)=O)CC[C@@H](C)[C@H](C)[C@H]5C4=CC[C@@H]3[C@]21C WCGUUGGRBIKTOS-GPOJBZKASA-N 0.000 description 1
- 244000089742 Citrus aurantifolia Species 0.000 description 1
- 235000008733 Citrus aurantifolia Nutrition 0.000 description 1
- 206010011878 Deafness Diseases 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 238000012449 Kunming mouse Methods 0.000 description 1
- OBIOZWXPDBWYHB-UHFFFAOYSA-N Nobiletin Natural products C1=CC(OC)=CC=C1C1=C(OC)C(=O)C2=C(OC)C(OC)=C(OC)C(OC)=C2O1 OBIOZWXPDBWYHB-UHFFFAOYSA-N 0.000 description 1
- 235000011941 Tilia x europaea Nutrition 0.000 description 1
- 206010067584 Type 1 diabetes mellitus Diseases 0.000 description 1
- 230000036982 action potential Effects 0.000 description 1
- 150000001298 alcohols Chemical class 0.000 description 1
- VQKFNUFAXTZWDK-UHFFFAOYSA-N alpha-methylfuran Natural products CC1=CC=CO1 VQKFNUFAXTZWDK-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000008033 biological extinction Effects 0.000 description 1
- 230000017531 blood circulation Effects 0.000 description 1
- 210000000133 brain stem Anatomy 0.000 description 1
- 230000023852 carbohydrate metabolic process Effects 0.000 description 1
- 235000021256 carbohydrate metabolism Nutrition 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 235000015165 citric acid Nutrition 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 210000003477 cochlea Anatomy 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000016097 disease of metabolism Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 210000005069 ears Anatomy 0.000 description 1
- 230000000763 evoking effect Effects 0.000 description 1
- HVQAJTFOCKOKIN-UHFFFAOYSA-N flavonol Natural products O1C2=CC=CC=C2C(=O)C(O)=C1C1=CC=CC=C1 HVQAJTFOCKOKIN-UHFFFAOYSA-N 0.000 description 1
- 150000002216 flavonol derivatives Chemical class 0.000 description 1
- 235000011957 flavonols Nutrition 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000010370 hearing loss Effects 0.000 description 1
- 231100000888 hearing loss Toxicity 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 230000003914 insulin secretion Effects 0.000 description 1
- 239000007928 intraperitoneal injection Substances 0.000 description 1
- JEIPFZHSYJVQDO-UHFFFAOYSA-N iron(III) oxide Inorganic materials O=[Fe]O[Fe]=O JEIPFZHSYJVQDO-UHFFFAOYSA-N 0.000 description 1
- CJWQYWQDLBZGPD-UHFFFAOYSA-N isoflavone Natural products C1=C(OC)C(OC)=CC(OC)=C1C1=COC2=C(C=CC(C)(C)O3)C3=C(OC)C=C2C1=O CJWQYWQDLBZGPD-UHFFFAOYSA-N 0.000 description 1
- 150000002515 isoflavone derivatives Chemical class 0.000 description 1
- 235000008696 isoflavones Nutrition 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 239000004571 lime Substances 0.000 description 1
- 239000007791 liquid phase Substances 0.000 description 1
- 208000030159 metabolic disease Diseases 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 238000010172 mouse model Methods 0.000 description 1
- MRIAQLRQZPPODS-UHFFFAOYSA-N nobiletin Chemical compound C1=C(OC)C(OC)=CC=C1C1=CC(=O)C2=C(OC)C(OC)=C(OC)C(OC)=C2O1 MRIAQLRQZPPODS-UHFFFAOYSA-N 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 230000005994 pancreas dysfunction Effects 0.000 description 1
- 239000000825 pharmaceutical preparation Substances 0.000 description 1
- 229940127557 pharmaceutical product Drugs 0.000 description 1
- WRLGYAWRGXKSKG-UHFFFAOYSA-M phenobarbital sodium Chemical compound [Na+].C=1C=CC=CC=1C1(CC)C(=O)NC([O-])=NC1=O WRLGYAWRGXKSKG-UHFFFAOYSA-M 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000011514 reflex Effects 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 150000003648 triterpenes Chemical class 0.000 description 1
- 229940096998 ursolic acid Drugs 0.000 description 1
- PLSAJKYPRJGMHO-UHFFFAOYSA-N ursolic acid Natural products CC1CCC2(CCC3(C)C(C=CC4C5(C)CCC(O)C(C)(C)C5CCC34C)C2C1C)C(=O)O PLSAJKYPRJGMHO-UHFFFAOYSA-N 0.000 description 1
- MWOOGOJBHIARFG-UHFFFAOYSA-N vanillin Chemical compound COC1=CC(C=O)=CC=C1O MWOOGOJBHIARFG-UHFFFAOYSA-N 0.000 description 1
- 210000003462 vein Anatomy 0.000 description 1
- 230000003442 weekly effect Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/73—Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2200/00—Function of food ingredients
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/35—Extraction with lipophilic solvents, e.g. Hexane or petrol ether
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Botany (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Animal Behavior & Ethology (AREA)
- Medicinal Chemistry (AREA)
- Biotechnology (AREA)
- Alternative & Traditional Medicine (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Medical Informatics (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Nutrition Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Medicines Containing Plant Substances (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention belongs to active constituents of medicine extraction and separation technology field, one kind is disclosed using proso millet prolamin as raw material, using ethanol extracting n-butyl alcohol, the method to prepare proso millet prolamin n-butanol extract.One of proso millet prolamin extract active ingredient that the present invention is prepared is flavone compound, and this compound structure is similar with wild scutellaria glycosides structure.The proso millet prolamin n-butanol extract prepared can effectively reduce the fasting blood-glucose of diabetes, put on weight, and alleviate many food diuresis of many drinks.Available for treatment/alleviation of the hyperglycaemia as caused by diabetes, weight loss and " more than three " symptom, available for reparation mouse hearing impairment.The present invention provides new approaches for hypoglycemic and protection hearing, the exploitation for the medicine for repairing hearing impairment.
Description
Technical field
The invention belongs to medicinal active ingredient extraction and separation technology and food, pharmaceutical product use technical field, it is related to Herba pogonatheri criniti
The extracting and developing preparation method of certain kind of berries Flavonoid substances.The proso millet prolamin n-butanol extract prepared can reduce blood glucose, change
Become thin caused by kind diabetes, improve diabetes " more than three " symptom, repair hearing impairment.
Background technology
Proso millet prolamin is rose family strawberry plants, and alias rust hair strawberry, yellowhairy strawberry herb are grown in height above sea level 700-3000m's
Patana or limes marginis sylvan life, aboundresources, are distributed in the ground such as Yunnan Province of China, Guangxi, Sichuan, Guizhou, there is clearing heat and detoxicating, promoting blood circulation
Effect of dissolving stasis, with preferable medical value.Diabetes be it is a kind of as caused by heredity or day after tomorrow defect of insulin secretion with
The metabolic disease that carbohydrate metabolism disturbance and hyperglycaemia are characterized.Diabetic persistently raises along with blood glucose, and body weight drastically subtracts
Gently, liver, kidney, pancreas dysfunction.In addition, the disorderly metabolism of diabetic often produces a large amount of oxygen radicals, cause other
The generation of complication.Yet there are no proso millet prolamin has reduction blood glucose, improves and become thin caused by diabetes, improve diabetes
" more than three " symptom, the report for repairing hearing impairment.
The content of the invention
Present invention firstly provides the extracting method of proso millet prolamin n-butanol extract, using proso millet prolamin as raw material, second is used
Alcohol-extracting n-butyl alcohol, to prepare proso millet prolamin n-butanol extract.The method of the present invention is simple to operate, the yellow hair prepared
Fragaia ananassa Duchesne extract has the effect of substantially reduction blood glucose.
The preparation method for the proso millet prolamin n-butanol extract that the present invention is provided, the described method comprises the following steps:
(1) shredded after proso millet prolamin is dried, using alcohol steep, obtain proso millet prolamin alcohol steep thing;
(2) by after the proso millet prolamin extract evaporation in step (1), supernatant is filtered to take, proso millet prolamin ethanol is obtained and slightly carries
Thing;
(3) extracted after the proso millet prolamin alcohol extracts in step (2) are mixed with enough n-butanols, obtain proso millet prolamin
N-butanol extract;
(4) the proso millet prolamin n-butanol extract in step (3) is evaporated, after n-butanol volatilization is clean, obtains yellow hair
Used after strawberry n-butanol extract, dilution.
In step (1), the temperature of the drying is 25-37 DEG C;Preferably, it is 32 DEG C.
In step (1), the concentration of alcohol is 50%-95% (volume fraction);Preferably, it is 75%.
In step (1), the temperature of the extraction is 18-32 DEG C;Preferably, it is 25 DEG C (room temperatures).
In step (1), the time of the extraction is 2-4h;Preferably, it is 3h.
In step (1), the amount ratio of the proso millet prolamin and ethanol is (6-20) g:(300-600)ml;Preferably, it is
6g:400ml.
In step (1), the proso millet prolamin includes root, stem, leaf or its mixture of yellow hair proso millet prolamin plant.
In step (2), the evaporation can be using Rotary Evaporators evaporation.
In step (2), the time of the evaporation is 1-2 hours;Preferably, it is 1.5 hours.
In step (2), the evaporating temperature is 35 DEG C -45 DEG C;Preferably, it is 40 DEG C.
In step (2), the crude drug concentration of the yellow hair proso millet prolamin alcohol extracts thing can be 0.5g/ml;Wherein, institute
State crude drug concentration and refer to evaporation gained original liquid concentration.
In step (2), preferably under vacuo under the conditions of carry out.
In step (3), the consumption of n-butanol is 15~20 times (V/V) of proso millet prolamin alcohol extracts.
In step (3), the temperature of the extraction is 18-32 DEG C;Preferably, it is 25 DEG C (room temperatures).
In step (3), the time of the extraction is 2-4h;Preferably, it is 3h.
In step (3), the n-butanol can be substituted by ethyl acetate.
In step (4), the evaporation can be using Rotary Evaporators evaporation.
In step (4), preferably under vacuo under the conditions of carry out.
In step (4), the temperature of the evaporation is 50 DEG C -65 DEG C;Preferably, it is 60 DEG C.
In step (4), the time of the evaporation is 1-2 hours;Preferably, it is 1.5 hours.
In step (4), it is generally the case that when the volume of remaining proso millet prolamin n-butanol extract in Rotary Evaporators and Huang
When hair strawberry alcohol extracts volume is identical, n-butanol is that volatilization is complete.
In step (4), the concentration of the dilution can be:0.4g/ml and 0.8g/ml etc..
In step (4), the active ingredient of the yellow hair proso millet prolamin n-butanol extract is Flavonoid substances, structure and open country
Scutellaria glycosides is similar.
After step (4) terminates, step (5) can also be included:N-butanol extracting liquid in step (4) is subjected to freezing dry
It is dry, obtain proso millet prolamin extract dry powder.
In the present invention, Flavonoid substances are soluble in ethanol and n-butanol, are dissolved in after ethanol, and ethanol evaporation obtains Huang
Letones and it is other dissolve in second alcohols material, further extracted using n-butanol, can will be partially soluble in ethanol but not dissolve in
N-butanol debris is removed.
Present invention also offers a kind of proso millet prolamin n-butanol extract or its dry powder, by being carried to proso millet prolamin n-butanol
Thing or its dry powder is taken to carry out Active Components, the main component of the proso millet prolamin extract is similar with wild scutellaria glycosides structure
Flavone compound.
Present invention also offers a kind of proso millet prolamin n-butanol extract or its dry powder prepared by the above method, institute
The main component for stating proso millet prolamin n-butanol extract or its dry powder is flavone compound.Wherein, wild scutellaria glycosides structure is as follows
Shown in formula (I):
Present invention also offers a kind of identification of proso millet prolamin n-butanol extract active ingredient and separation method, including with
Lower step:
(a) the proso millet prolamin extract dry powder for preparing above method step (5) is dissolved in alcohol, adds corresponding reagent and determines
Absorbance is surveyed after appearance, reference standard determines dry powder general flavone content.
(b) proso millet prolamin flavones semifinished product is dissolved in methanol in step (a), and its species is identified using thin layer.
(c) proso millet prolamin flavones semifinished product HPLC is analyzed and is collected active ingredient in step (5)
In step (a), the dry powder is dissolved in 60% ethanol, mixes that (volume ratio is 500 with NaNO2 (5%):1),
After 6min, 1ml 10%AlCl3 and 10mLNaOH (1M) are added, mixture is finally settled to 25ml with 60% ethanol, shaken up,
Place 15min.
In step (a), the absorbance is determined at 500nm wavelength.
In step (a), the standard items are rutin.
In step (c), the HPLC chromatogram post is:Eclipse XDB-C18 (4.6 × 250mm, 5mm), mobile phase:First
The phosphoric acid solution of alcohol -0.06% (50:50), flow velocity:1ml/min, Detection wavelength:360nm, column temperature:25 DEG C, sample size:10ml.
In step (c), the standard items are wild scutellaria glycosides.
In step (c), the sample and standard items after filtering with microporous membrane 2 times with using.
Hypoglycemic related food/health products and medicine are being prepared the invention provides the proso millet prolamin n-butanol extract
Application in thing.
Cause the food/guarantor become thin in preparation improvement diabetes the invention provides the proso millet prolamin n-butanol extract
Application in strong product and medicine.
The invention provides the proso millet prolamin n-butanol extract prepare improve diabetes " more than three " symptom food/
Application in health products and medicine.Referring to many drinks, many foods, diuresis described three more.
The invention provides the proso millet prolamin n-butanol extract in the food for preparing protection hearing, repairing hearing impairment
Application in product/health products and medicine.
The beneficial effects of the present invention are the method that the present invention prepares proso millet prolamin n-butanol extract is simple to operate, obtains
To the important component of proso millet prolamin extract be flavone compound, its structure is similar to wild scutellaria glycosides.The inventive method system
The blood glucose in diabetic mice rise tool that standby proso millet prolamin extract is induced STZ is significantly reduced effect, and its concentration is higher,
Reduction effect is more obvious.The inventive method prepare proso millet prolamin extract STZ induce diabetic mice body weight mitigation, it is many
" more than three " symptom of many drink diuresis of food has significant mitigation, and its concentration is higher, and reduction effect is more obvious.The inventive method system
Standby proso millet prolamin extract has significant restitution to hearing impairment mouse hearing ability, and its concentration is higher, and reduction is made
With more obvious.
Brief description of the drawings
Fig. 1 is influence of the proso millet prolamin n-butanol extract of embodiment 3 to STZ mouse fasting blood sugars.
Fig. 2 be the proso millet prolamin n-butanol extract of embodiment 4 to STZ mouse weights, drinking-water, food ration influence;A is Huang
Influence of the hair strawberry n-butanol extract to STZ mouse amounts of drinking water;B is proso millet prolamin n-butanol extract to STZ mouse weights
Influence;C is influence of the proso millet prolamin n-butanol extract to STZ mouse weight food rations.
Fig. 3 is the rutin standard curve of embodiment 5.
Fig. 4 is thin-layer chromatogram;Wherein, 1. Quercetin, 2. wild scutellaria glycosides, 3. Nobiletins, 4. sample liquids.
Fig. 5 is the HPLC result figures of wild scutellaria glycosides and proso millet prolamin n-butanol extract;A is wild scutellaria glycosides, and B is Herba pogonatheri criniti
Certain kind of berries n-butanol extract.
Embodiment
With reference to specific examples below and accompanying drawing, the present invention is described in further detail.The process of the implementation present invention,
Condition, experimental method etc., are the universal knowledege and common knowledge of this area, this hair in addition to the following content specially referred to
It is bright that content is not particularly limited.
The preparation of the proso millet prolamin n-butanol extract of embodiment 1
Proso millet prolamin is dried into complete stool (including root, stem, leaf) chopping, 75% ethanol 400ml was extracted after 3 hours at room temperature,
It is fully transferred in Rotary Evaporators, boiling 1.5h under vacuum filters to take supernatant, the Huang that crude drug concentration is 0.5g/ml is made
Hair strawberry alcohol extracts.
Room temperature extracts 3h after proso millet prolamin alcohol extracts are mixed with enough n-butanols, is transferred in Rotary Evaporators,
Boiling under 60 DEG C of vacuum, n-butanol is volatilized net, obtains proso millet prolamin n-butanol extract.
Freeze drier is transferred to after proso millet prolamin n-butanol extract is freezed, proso millet prolamin flavones dry powder is obtained.
Diabetic mice (STZ mouse) model construction of embodiment 2STZ inductions
Mice by intraperitoneal injection Streptozotocin (STZ) sets up type 1 diabetes model.Press 10mlkg-1Volume injection,
Normal group injects pH4.6 sodium citrate cushioning liquid, and model group presses 40mgkg-1Dosage injects 0.4%STZ- citric acids
Sodium solution, 1 time/d, continuously injects 5d, model stability after STZ is injected 14 days, continuous fasting measures fasting blood sugar > in 6 hours
12mmol·L-1It is considered as modeling success, is then discarded less than this value.
Influence of the proso millet prolamin n-butanol extract of embodiment 3 to STZ mouse fasting blood-glucoses
Each group mouse presses 10mLkg-1Volume gavage, normal group and model group gavage distilled water, low dose group, high agent
Amount group presses 0.4gmL respectively-1With 0.8g/ml gavage proso millet prolamin n-butanol extracts, 1 time/d, totally 4 weeks.Mouse is determined weekly
Tail vein blood after blood glucose, fasting 6h, determines fasting blood-glucose.
Experimental result as shown in figure 1, proso millet prolamin n-butanol extract can significantly reduce diabetic mice fasting blood sugar,
And concentration more high effect is more obvious.As a result show that proso millet prolamin n-butanol extract has the effect of reduction fasting blood-glucose.
The proso millet prolamin n-butanol extract of embodiment 4 to diabetic mice body weight, ingest, the influence of amount of drinking water
As described in Example 3 handle after, respectively determine mouse body weight, ingest, amount of drinking water;Experimental result such as Fig. 2 institutes
Show, proso millet prolamin n-butanol crude extract can effectively improve the symptom of diabetic mice body weight mitigation, hence it is evident that improve its " more than three " disease
Shape.As a result illustrate that proso millet prolamin extract has and increase diabetic mice body weight, improve the effect of its many many food of drink
The constituent analysis of the proso millet prolamin n-butanol extract dry powder of embodiment 5
The proso millet prolamin extract dry powder that precision weighs the preparation of embodiment 1 is dissolved in 60% alcohol, takes 500mL and 1mL
NaNO2 (5%) is mixed, after 6min, 1mL 10%AlCl3 and 10mLNaOH (1M) is added, finally by 60% ethanol of mixture
25ml is settled to, is shaken up, 15min is placed, absorbance, using 60% ethanol as blank control group, rutin are measured at 500nm wavelength
For reference standard, dry powder general flavone content is demarcated with rutin standard curve.Fig. 3 is rutin standard curve, y=3.9064x+
0.0021, R2=0.9991.
Proso millet prolamin n-butanol extract dry powder is mixed with vanillic aldehyde glacial acetic acid (5%, w/v, 0.5mL), 1mL perchloric acid,
60 DEG C are incubated after 10min, ice bath 15min, then add 5mL glacial acetic acid and mix, extinction is read under 538nm wavelength after 6min
Degree.Using ursolic acid as standard items, dry powder total triterpene contentses are determined according to its standard curve.
As shown in table 1, the main component of proso millet prolamin n-butanol extract dry powder is flavone compound to experimental result.
The effective sugar-lowering components detection of the proso millet prolamin n-butanol extract dry powder of table 1.
Flavones Identification of Species in the proso millet prolamin n-butanol extract of embodiment 6
1) flavones species is determined using chromogenic reaction.
The chromogenic reaction of sample liquid:Proso millet prolamin n-butanol extract prepared by 2ml embodiments 1 is taken in test tube, in ripple
Observed under long 254nm uviol lamps, record color.A little sample liquid is dipped with filter paper, further determining that color with glass bar again
And record.
Ammoniacal liquor reacts:2ml sample liquids and 2ml ammoniacal liquor are taken in test tube, is mixed, is observed under 254nm uviol lamps.Walk below
The rapid chromogenic reaction with sample liquid.
Sodium hydroxide reacts:2ml sample liquids and 2ml NaOH solutions are taken in test tube, is mixed, is observed under 254nm lamps.
Chromogenic reaction of the following steps with sample liquid.
Alchlor reacts:The alchlor ethanol of 2ml sample liquids and 2ml 1% is taken, is mixed, is observed at 254nm.
Chromogenic reaction of the following steps with sample liquid.
2) thin layer chromatography analysis flavones species is used
2mg/ml proso millet prolamin n-butanol extract, wild scutellaria glycosides, Nobiletin, rutin, Quercetin is prepared with methanol
Reference substance solution is standby.From different exhibition coating systems, such as table 2.Distinguish pipette samples and reference substance solution with capillary, be added drop-wise to
On silica gel plate, hair-dryer drying, repeatedly point sample three times.Silica gel plate is positioned in the exhibition layer cylinder containing developing agent after point sample, Yu Zi
Observe, record under External Observation instrument, calculate Rf values.
Rf=X/L
Formula (II)
In formula (II), X is the flavone compound displacement (cm) isolated;L is the distance (cm) that solvent is moved.
Thin-layer chromatography development system is as shown in table 2;Thin-layer chromatography result is as shown in figure 4, wherein, 1. Quercetins, 2. open countries are yellow
Cen glycosides, 3. Nobiletins, 4. sample liquids;As seen from Figure 4, proso millet prolamin n-butanol extract of the invention and wild scutellaria glycosides
Displacement is the most close, can be learnt by calculating its Rf value, proso millet prolamin n-butanol extract and wild scutellaria glycosides structure phase
Seemingly, table 4 further illustrates that both structures are similar.
As shown in table 3, flavonoid species may in proso millet prolamin n-butanol extract for chromogenic reaction experimental result
It is flavones, flavonols or isoflavones.
Thin-layer chromatography experimental result is as shown in table 4, identical with wild scutellaria glycosides when sample liquid Rf values are 0.35, and is shone ultraviolet
Penetrate lower presentation yellow-green fluorescence, therefore contain flavones and flavonoid drugs in sample, and structure is identical with open country scutellaria glycosides or phase
Seemingly.
The thin-layer chromatography development system of table 2.
The chromogenic reaction of the flavone compound of table 3.
The Rf values of the flavone compound of table 4.
Flavones species in the high-efficient liquid phase chromatogram technique analysis proso millet prolamin n-butanol extract of embodiment 7
High performance liquid chromatography parameter is:Chromatographic column:Eclipse XDB-C18 (4.6 × 250mm, 5 μm), mobile phase:First
The phosphoric acid solution of alcohol -0.06% (50:50), flow velocity:1ml/min, Detection wavelength:360nm, column temperature:25 DEG C, sample size:10μl.Will
Sample and standard items after filtering with microporous membrane 2 times with using.
Using wild scutellaria glycosides as standard items, proso millet prolamin n-butanol extract prepared by embodiment 1 is analyzed.
Experimental result is as shown in figure 5, wherein A is wild scutellaria glycosides result figure, and B is proso millet prolamin n-butanol extract result
Figure.Baseline is steady at 360 nm, and wild scutellaria glycosides retention time 5.296min, sample liquid has two peaks in 5.258,5.727min,
Close with wild scutellaria glycosides retention time, both total peak area ratios are 64.5%, account for crude extract main component.Show Herba pogonatheri criniti
Contain the material similar with wild scutellaria glycosides structure in certain kind of berries n-butanol extract, collection 5-6min is washed under the conditions of can collecting this separation
Go out liquid, be the effective sugar-lowering components of proso millet prolamin.Open country scutellaria glycosides is hypoglycemic at present and protection liver effect does not have been reported that also.
The structure of the hearing impairment mouse model of embodiment 8
After 50 kunming mice adaptability are raised one week, random selection 10 is only used as normal group, remaining mouse exposed to 4~
In 45kHZ, 110dBSPL white noise, 9h is exposed daily, 10d is exposed altogether, auricle reflex, i.e. auricle hold up to adapt to glouglou
After the phenomenon of sound is eliminated, model group, flunarizine hydrochloride group, low dose group, high dose group are randomly divided into.
Influence of the proso millet prolamin n-butanol extract of embodiment 9 to mouse hearing ability
Each group mouse presses 10mLkg-1Volume gavage, normal group and model group gavage distilled water, flunarizine hydrochloride group
Gavage 1.5X 10-3g·kg-1, low dose group and high dose group gavage 0.4gkg-1And 0.8gkg-1Huang prepared by embodiment 1
Hair strawberry 1 time/d of n-butanol extract, totally 4 weeks.After gavage terminates, each group Cochlea of Mouse electrograph (ECochG), listening property brain stem are surveyed
React (ABR).
Mouse peritoneal injection 4mg/kg sodium phenobarbital liquid is anaesthetized, using the multi-functional evoked potentuial measuring systems of YSSD-1000,
Stimulation sound is shortwave, and sound pressure level 90dB, sensitivity 6.2uv, band logical 100~1000HZ of frequency carry out ABR and ECochG experiments.
Record phase and complex action potential (CAP) amplitude variations between mouse ears each ripple incubation period, ripple.
Influence result to CAP amplitudes is as shown in table 5, compared with model group, (low dose of proso millet prolamin n-butanol extract group
Amount group and high dose group) CAP amplitudes substantially rise.As a result illustrate that proso millet prolamin n-butanol extract can significantly recover mouse hearing
Damage, and concentration more high effect is more obvious.
Influence result to the phase between incubation period and ripple is as shown in table 6, compared with model group, proso millet prolamin n-butanol extract
Group (low dose group and high dose group) between I, III ripple incubation period and I-IV, I-V ripple the phase have notable recovery.Illustrate that embodiment 1 is made
Standby proso millet prolamin n-butanol extract can be obviously improved auditory center function.As a result proso millet prolamin n-butanol extract pair is shown
Noise hearing loss has therapeutic action.
The proso millet prolamin n-butanol extract of 5. embodiment of table 1 to CAP amplitudes influence (N=10)
Note:Compared with model group,*P < 0.05,*P < 0.01
The proso millet prolamin n-butanol extract of 6. embodiment of table 1 to the phase between incubation period and ripple influence (N=10)
Note:Compared with model group,*P < 0.05,*P < 0.01
The protection content of the present invention is not limited to above example.Under the spirit and scope without departing substantially from inventive concept, this
Art personnel it is conceivable that change and advantage be all included in the present invention, and using appended claims as protect
Protect scope.
Claims (10)
1. a kind of preparation method of proso millet prolamin n-butanol extract, it is characterised in that the described method comprises the following steps:
(1) shredded after the whole strain of proso millet prolamin is dried, using alcohol steep, obtain proso millet prolamin alcohol steep thing;
(2) by after the proso millet prolamin extract evaporation in step (1), supernatant is filtered to take, proso millet prolamin alcohol extracts are obtained;
(3) extracted after the proso millet prolamin alcohol extracts in step (2) are mixed with enough n-butanols, obtain the positive fourth of proso millet prolamin
Alcoholic extract;
(4) the proso millet prolamin n-butanol extract in step (3) is evaporated, obtains proso millet prolamin n-butanol extracting liquid.
2. method as claimed in claim 1, it is characterised in that in step (1), the concentration of the ethanol is 50%-95%;Institute
Dry temperature is stated for 25-37 DEG C;The temperature of the extraction is 18-32 DEG C, and the time of the extraction is 2-4h.
3. method as claimed in claim 1, it is characterised in that in step (1), the amount ratio of the proso millet prolamin and ethanol is
(6-20)g:(300-600)ml.
4. method as claimed in claim 1, it is characterised in that in step (2), the temperature of the evaporation is 35 DEG C -45 DEG C;Institute
The time for stating evaporation is 1-2 hours;In step (3), the consumption of n-butanol is 15~20 times of (V/ of proso millet prolamin alcohol extracts
V)。
5. method as claimed in claim 1, it is characterised in that in step (3), the temperature of the extraction is 18-32 DEG C;It is described
The time of extraction is 2-4h;In step (4), the temperature of the evaporation is 50 DEG C -65 DEG C;The time of the evaporation is 1-2 hours.
6. the proso millet prolamin n-butanol extract that a kind of any one methods described such as claim 1-5 is prepared.
7. proso millet prolamin n-butanol extract as claimed in claim 6 is in hypoglycemic food/health products and medicine are prepared
Application.
8. proso millet prolamin n-butanol extract as claimed in claim 6 causes the food/guarantor become thin in preparation improvement diabetes
Application in strong product and medicine.
9. proso millet prolamin n-butanol extract as claimed in claim 6 prepare improve diabetes " more than three " symptom food/
Application in health products and medicine.
10. proso millet prolamin n-butanol extract as claimed in claim 6 is in the food for preparing protection hearing, repairing hearing impairment
Application in product/health products and medicine.
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