CN1071698A - Produce Sodium Glutamate with double-enzyme method sugar making - Google Patents
Produce Sodium Glutamate with double-enzyme method sugar making Download PDFInfo
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- CN1071698A CN1071698A CN 91109741 CN91109741A CN1071698A CN 1071698 A CN1071698 A CN 1071698A CN 91109741 CN91109741 CN 91109741 CN 91109741 A CN91109741 A CN 91109741A CN 1071698 A CN1071698 A CN 1071698A
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Abstract
The invention discloses a kind of method of producing Sodium Glutamate with double-enzyme method sugar making.Be to utilize α-Dian Fenmei, after go out enzyme and cooling, carry out saccharification with saccharifying enzyme at short notice with starch liquefacation, behind the enzyme that goes out, use activated carbon decolorizing then again, hydrolyzed solution glucose purity (DE) value reaches more than 98, and transformation efficiency reaches more than 107%, and reducing sugar is more than 30%.Improved filterableness.Can be used for the sugaring of glutamic acid fermentation and glutamic acid fermentation stream.Have the hydrolysis specificity, hydrolysis is complete, does not have side reaction, has simplified fermention medium, has improved yield, and has alleviated the corrosion to equipment greatly, has reduced the renewal and renovation of equipment maintenance cost.
Description
The present invention relates to the production method of Sodium Glutamate, relate in particular to the improvement of method for manufacturing sugar in the Sodium Glutamate production process.
China mainly is to be raw material production Sodium Glutamate (monosodium glutamate) with starch at present, aspect production method, all be to adopt starch is carried out acid hydrolysis system glucose, add urea, phosphorus, magnesium, potassium, vitamin H and following bacterial classification then, ferment, again through ion-exchange, the last Sodium Glutamate (monosodium glutamate) that gets of Crystallization Separation.Hydrochloric acid, caustic soda that acid system hydrolysis system glucose is consumed are more, and hydrolyzed PH value reaches 1.5, and be therefore very serious to the corrosion of equipment, causes renewal and renovation of equipment and maintenance all to expend very big.In addition, under the hydrolysising condition of optimum, the glucose purity of gained still can not be higher than DE value 92, and main is that hydrolysis sugar concentration has only 17～22%, can not adapt to the processing requirement of present glutamic acid fermentation stream sugaring.
The purpose of this invention is to provide a kind of with double-enzyme method sugar making and the production method that is used to produce Sodium Glutamate, make hydrolysis have unicity, hydrolysis fully and do not have a side reaction, hydrolyzation of glucose purity height, concentration are big, improve yield, can in the identical time, produce more L-glutamic acid.
The object of the present invention is achieved like this: produce Sodium Glutamate with double-enzyme method sugar making, in starch, add water, α-Dian Fenmei is sized mixing, transfer pH value to be acid, the enzyme that goes out that after liquefaction, heats up, cooling then, add saccharifying enzyme again and carry out saccharification, and then the enzyme that goes out that heats up, get G/W after the decolouring, again fermentation, through ion-exchange, Crystallization Separation, and then obtain Sodium Glutamate.
The invention will be further described below in conjunction with accompanying drawing.Fig. 1 is a process flow sheet of producing the prior art of monosodium glutamate, Fig. 2 is a process flow sheet of the present invention, according to Fig. 2, starch is added water furnishing powder slurry, its concentration is 30～40%(W/W), preferably 40%, add α-Dian Fenmei then, BF-7658 liquid starchy enzyme preferably, consumption is 8～11 units/gram starch, add calcium chloride and transfer pH value to 6.0～6.7, preferably 6.4.Squeeze in the Jet liquefier with pump.Under 88～90 ℃, it is fast and even that the turbulent flow that vapo(u)r blasting produces is heated starch, and viscosity reduces fast.The starch slurry of liquefaction is drawn off by the injector below, and introducing continuously, liquefaction Guan Zhongyue reached the liquefaction degree that needs in 40 minutes.Then be rapidly heated to 115～125 ℃ of enzymes that go out, preferably 125 ℃, the time is 2～5 minutes.And then cooling fast, in 2 seconds, temperature is reduced to below 70 ℃ with water coolant.Liquefier after the liquefaction is added hydrochloric acid transfer pH value to 4.2～4.5, be preferably 4.4, under agitation condition, add saccharifying enzyme.Used saccharifying enzyme preferably changes the saccharifying enzyme of glucoside enzyme with removing glucose, and add-on is 80～100 units/gram starch preferably.Under 60 ℃ of temperature, adopt at a slow speed stir, the quiet shape reaction method of short-term carries out saccharification, saccharification time is 8～15 hours, is warming up to 80～100 ℃ then, 80 ℃ of enzymes that go out preferably, the time kept 20～30 minutes.Be preferably 30 minutes.Add activated carbon at last and under 70～80 ℃ of temperature, decolour, promptly get the G/W that is used to ferment.This liquid glucose coloring matter growing amount is few, and filterableness is very good, reaches 250 liters/decimeter
2More than, dextrose equivalent value is more than 98, all the other produce the same of continuous technology and acid system hydrolysis system Sodium Glutamate under Sodium Glutamates.Hydrolysis of the present invention has specificity, and hydrolysis is complete and do not have side reaction, and filterableness is good.Use enzymic hydrolysis starch metabolism product more simultaneously, enzyme itself is again a protein, and the nutritive ingredient of hydrolyzed solution is abundanter, can simplify fermention medium greatly, and yield is also high.Also alleviated corrosion greatly, reduced and upgrade maintenance cost equipment.
Be several embodiments of the present invention below.Embodiment 1, gets 100kg starch, adds water 250kg, and furnishing powder slurry adds BF-7658 liquid starchy enzyme, and consumption is 10 units/gram starch, adds calcium chloride again and transfers pH value to 6.4.Squeeze in the Jet liquefier with pump, heat with steam that to make temperature be 90 ℃, the starch slurry of liquefaction is drawn off by the injector below, introduces continuously liquefaction Guan Zhongyue 40 minutes, reaches the liquefaction degree that needs.Then be rapidly heated to 125 ℃ of enzymes that go out, 2 minutes time, and then cooling fast, to finish in 2 seconds, temperature is reduced to 70 ℃.Add Hcl in the liquefier after the liquefaction and transfer pH value to 4.4, add under agitation condition and remove the saccharifying enzyme that glucose changes the glucoside enzyme, consumption is preferably 100 units/gram starch, and saccharification temperature is 60 ℃, and saccharification time is 8 hours.Be warming up to 80 ℃ of enzymes that go out with steam then, kept 30 minutes.Add decolorizing with activated carbon, the time is 30 minutes.The grape grain sugar value DE that gets Glucose Liquid is 99, and filterableness is 340 liters/decimeter
2, transformation efficiency 109%, reducing sugar 33%, 68 ° of printing opacities are used to produce Sodium Glutamate, and the technology of the condition of fermentation and acid system sugaring is identical, must produce acid 7.51, transformation efficiency 49.7, foam is normal, and fermentation time is 38 hours.
Embodiment 2, get 100kg starch, furnishing concentration is 30% powder slurry, adds BF-7658 liquid starchy enzyme, 8 units/gram starch, transferring pH value is 6.0, and the enzyme-removal temperature after the liquefaction is 115 ℃, keeps 5 minutes, transfer pH value to 4.2 before the saccharification, the saccharifying enzyme consumption is 80 units/gram starch, and saccharification time is 10 hours, and all the other conditions are with embodiment 1.The sugar value DE value that gets Glucose Liquid is 98.3.Filterableness is 250 liters/decimeter
2, transformation efficiency is 107%, reducing sugar 30%, 72 ° of printing opacities.
Embodiment 3, and transferring pH value when sizing mixing is 6.7, the powder slurry of furnishing 35%, and the α-Dian Fenmei consumption is 11 units/gram starch, heats up that to go out that enzyme holds time be 5 minutes, and transferring PH before the saccharification is 4.5, and saccharification time is 15 hours, and all the other conditions are identical with embodiment 1.Getting the DE value is 98, and transformation efficiency is 109%, reducing sugar 32.4%, 500 liters/decimeter of filterablenesses
2, printing opacity is 59.5 °, and producing acid is 7.05, and transformation efficiency is 50.18, and foam is normal, and fermentation time is 40 hours.
Claims (17)
1, produces Sodium Glutamate with double-enzyme method sugar making, starch is got Sodium Glutamate with fermentation, ion-exchange, Crystallization Separation, it is characterized in that before fermentation, in starch, adding water, α-Dian Fenmei is sized mixing, it is acid that adjust pH is, with the enzyme that goes out that heats up after the liquefaction, cooling adds saccharifying enzyme again and carries out saccharification then, and then the enzyme that goes out that heats up, get G/W after the decolouring and be used for fermentation.
2, according to claim 1 with bisgallic acid method Closed Circulation in Sugar Production Sodium Glutamate, it is characterized in that said sizing mixing is to add water furnishing 30～40%(W/W) powder slurry in starch, add α-Dian Fenmei and calcium chloride then, transfer pH value to 6.0～6.7.
3, according to claim 1 with double-enzyme method sugar making production Sodium Glutamate, it is characterized in that said liquefaction is to introduce continuously the about liquefaction degree that reached needs in 40 minutes of liquefaction reaction process in the liquefaction pipe under 88～90 ℃ of temperature behind steam ejection liquefaction.
4, according to claim 1ly produce Sodium Glutamate with double-enzyme method sugar making, it is characterized in that intensification after the said liquefaction enzyme that goes out is that liquefier is warmed up to 115～125 ℃ of enzymes that go out, the time is 2～5 minutes.
5, according to claim 1 with double-enzyme method sugar making production Sodium Glutamate, it is characterized in that said cooling preferably makes liquefier be cooled to below 70 ℃ in 2 seconds.
6, according to claim 1 with double-enzyme method sugar making production Sodium Glutamate, it is characterized in that said saccharification is to add hydrochloric acid to transfer pH value to 4.2～4.5, adding saccharifying enzyme under the agitation condition at a slow speed then, adopting at a slow speed, stirring, the quiet shape reaction method of short-term carry out saccharification, saccharification temperature is 60 ℃, and the time is 8～15 hours.
7, produce Sodium Glutamate according to claim 1 is described with double-enzyme method sugar making, the intensification that it is characterized in that said saccharification liquid is gone out, and to require temperature be 80～100 ℃ to enzyme, and the time is 20～30 minutes.
8, according to claim 1 with double-enzyme method sugar making production Sodium Glutamate, it is characterized in that said decolouring is to add gac in saccharification liquid, carries out under 70～80 ℃ of temperature.
9, according to claim 1 and 2ly produce Sodium Glutamate, it is characterized in that said sizing mixing preferably adds water furnishing 40%(W/W in starch with double-enzyme method sugar making) the powder slurry.
10, according to claim 1 and 2 with double-enzyme method sugar making production Sodium Glutamate, it is characterized in that preferably BF-7658 liquid starchy enzyme of said α-Dian Fenmei.
11, according to claim 1 and 2ly produce Sodium Glutamate, it is characterized in that said accent pH value preferably 6.4 with double-enzyme method sugar making.
12, describedly produce Sodium Glutamate with double-enzyme method sugar making according to claim 1 or 4, it is characterized in that intensification after the said liquefaction enzyme that goes out preferably is warming up to 125 ℃ of enzymes that go out, the time is 2 minutes.
13, describedly produce Sodium Glutamate according to claim 1 or 6, it is characterized in that preferably adding hydrochloric acid earlier before the said saccharification transfers pH value to 4.4 with double-enzyme method sugar making.
14, describedly produce Sodium Glutamate according to claim 1 or 7, it is characterized in that intensification after the said saccharification enzyme that goes out preferably kept 30 minutes under 80 ℃ of temperature with double-enzyme method sugar making.
15, describedly produce Sodium Glutamate according to claim 1 or 6, it is characterized in that said saccharifying enzyme preferably changes the saccharifying enzyme of glucoside enzyme with removing glucose with double-enzyme method sugar making.
16, according to claim 10 with double-enzyme method sugar making production Sodium Glutamate, it is characterized in that the add-on of α-Dian Fenmei is preferably 8～10 units/gram starch.
17, according to claim 15ly produce Sodium Glutamate with double-enzyme method sugar making, the add-on that it is characterized in that said saccharifying enzyme is 80～100 units/gram starch preferably.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 91109741 CN1071698A (en) | 1991-10-12 | 1991-10-12 | Produce Sodium Glutamate with double-enzyme method sugar making |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 91109741 CN1071698A (en) | 1991-10-12 | 1991-10-12 | Produce Sodium Glutamate with double-enzyme method sugar making |
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| CN1071698A true CN1071698A (en) | 1993-05-05 |
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| CN 91109741 Pending CN1071698A (en) | 1991-10-12 | 1991-10-12 | Produce Sodium Glutamate with double-enzyme method sugar making |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1067433C (en) * | 1997-11-24 | 2001-06-20 | 河南莲花味之素有限公司 | Production of sugar from corn starch and glutamic acid fermentation |
| CN102154398A (en) * | 2010-12-28 | 2011-08-17 | 国润金华（北京）国际能源投资有限公司佛山高明分公司 | High-temperature and high-pressure cellulose-to-sugar process |
| CN105124518A (en) * | 2015-09-09 | 2015-12-09 | 湖州新天字味精有限公司 | Technology of producing monosodium glutamate from potato starch |
| CN107119091A (en) * | 2016-02-25 | 2017-09-01 | 安徽泰德康农业科技发展有限公司 | A kind of cassava whole-powder direct method method for manufacturing sugar |
| CN111567782A (en) * | 2020-06-17 | 2020-08-25 | 陆菊华 | Biological monosodium glutamate capable of reducing blood sugar |
-
1991
- 1991-10-12 CN CN 91109741 patent/CN1071698A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1067433C (en) * | 1997-11-24 | 2001-06-20 | 河南莲花味之素有限公司 | Production of sugar from corn starch and glutamic acid fermentation |
| CN102154398A (en) * | 2010-12-28 | 2011-08-17 | 国润金华（北京）国际能源投资有限公司佛山高明分公司 | High-temperature and high-pressure cellulose-to-sugar process |
| CN105124518A (en) * | 2015-09-09 | 2015-12-09 | 湖州新天字味精有限公司 | Technology of producing monosodium glutamate from potato starch |
| CN105124518B (en) * | 2015-09-09 | 2018-02-09 | 广东百味佳味业科技股份有限公司 | A kind of technique of potato starch manufacture monosodium glutamate |
| CN107119091A (en) * | 2016-02-25 | 2017-09-01 | 安徽泰德康农业科技发展有限公司 | A kind of cassava whole-powder direct method method for manufacturing sugar |
| CN111567782A (en) * | 2020-06-17 | 2020-08-25 | 陆菊华 | Biological monosodium glutamate capable of reducing blood sugar |
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