CN107163090A - A kind of extracting method of stevioside - Google Patents
A kind of extracting method of stevioside Download PDFInfo
- Publication number
- CN107163090A CN107163090A CN201710307983.7A CN201710307983A CN107163090A CN 107163090 A CN107163090 A CN 107163090A CN 201710307983 A CN201710307983 A CN 201710307983A CN 107163090 A CN107163090 A CN 107163090A
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- Prior art keywords
- steviol glycoside
- methanol
- stirring
- stevioside
- warming
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H1/00—Processes for the preparation of sugar derivatives
- C07H1/06—Separation; Purification
- C07H1/08—Separation; Purification from natural products
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H15/00—Compounds containing hydrocarbon or substituted hydrocarbon radicals directly attached to hetero atoms of saccharide radicals
- C07H15/20—Carbocyclic rings
- C07H15/24—Condensed ring systems having three or more rings
- C07H15/256—Polyterpene radicals
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- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
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- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
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Abstract
A kind of extracting method of stevioside, extracts that the mode production cycle length of stevioside, sewage quantity be big, the low problem of the small yield of yield, the present invention provides a kind of following technical scheme at present to overcome：（1）, crush stevia rebaudian leaf；（2）, diafiltration；（3）, decolourize；（4）, ultrafiltration；（5）, nanofiltration；（6）, filter liquor be concentrated into paste, add methanol, methanol content is reached more than 90%；（7）28 32 DEG C are warming up to, stirring is stood to there is crystallization to separate out；（8）Centrifuge, solid drying obtains thick steviol glycoside；（9）Thick steviol glycoside is put into ethanol, stood after stirring；（10）Centrifugal filtration is dried, and obtains steviol glycoside.The beneficial effects of the present invention are：Extract part and eliminate pectin, pigment, plant polyose first with 70% methanol and citric acid；Membrane separation technique removes the miscellaneous molecule of size；Save the purification process of resin adsorption；Shorten the production cycle；Improve the rate of output；Without waste water.
Description
Technical field
The present invention relates to a kind of production method of stevioside.
Background technology
Stevioside is also known as stevioside, and it is a kind of glucosides extracted from the leaf of feverfew, is natural low-heat
Measure sweetener, the calorific value of stevioside is only the 1/300 of sucrose, intake human body after do not absorbed, do not produce heat, be diabetes and
The sweetener that bariatric patients are applicable.When stevioside is with sucrose fructose or mixed isomerized sugar, its sugariness can be improved, improves mouth
Taste, available for candy, cake, beverage, solid beverage, fried pot foods, flavoring, preserved fruit.
As a kind of plant empty calory product of replacing sugar, steviol glycoside advantage is also very more：1. it is safe：Steviol glycoside is originated in
The edible existing centuries history of the resident on ground, finds no any murder by poisoning so far.2. low heat value：For make low-calorie diet,
Beverage, be highly suitable for diabetes, obesity, arteriosclerotic eat.3. steviol glycoside is soluble in water and alcohol, with sugarcane
Sugar, fructose, isomerized sugar etc. are used in mixed way taste more preferably.4. steviol glycoside belongs to non-fermented material, and property is stable, is difficult mould
Become, will not be changed in the making such as food, beverage, be also easy to accumulating.Long-term consumption will not cause carious tooth.5. steviol glycoside
Taste like sucrose, have again uniqueness it is refrigerant, sweet the characteristics of, available for making typical local food, candy etc., it is also possible to make flavouring.
Suppress some food, the peculiar smell of medicine, strange taste, be used for pharmacy, production syrup, electuary, pill instead of sucrose, it may also be used for adjust
Material, pickle product, toothpaste, cosmetics and cigarette etc..6. stability, under common food and drink processing conditions, steviol glycoside
Property be quite stable, advantageously reduce viscosity, bacteria growing inhibiting extends shelf life of products.
But the existing mode for extracting stevioside, there is more defect：1st, the production cycle is long, and seven talentes go out finished product；2nd, it is dirty
Water is big, and processing cost is high；3rd, invest big, producing cost is high；4th, yield is small, and labour's consumption is big；5th, yield is low, and cost is high.
The content of the invention
It is an object of the invention to provide a kind of extracting method of new stevioside, technical scheme is as follows：
A kind of extracting method of stevioside, described method comprises the following steps：
（1）, crush stevia rebaudian leaf, be put into tank；
（2）, add 70% methanol and citric acid diafiltration；
（3）, by after filtrate again micro- worry, cross resin decolorization；
（4）, clear liquid ultrafiltration again after decolouring；
（5）, nanofiltration again；
（6）, filter liquor be concentrated into paste, add methanol, methanol content is reached more than 90%；
（7）28-32 DEG C is warming up to, stirring is stood to there is crystallization to separate out；
（8）It is centrifuged, solid is dried after centrifugation, obtains thick steviol glycoside；
（9）Thick steviol glycoside is put into ethanol, then is warming up to 70 degrees Celsius, stirring is secondary to stand to there is crystallization to separate out；
（10）Dried again after centrifugal filtration, obtain steviol glycoside.
A kind of extracting method of stevioside, described method comprises the following steps：
（1）, crush stevia rebaudian leaf crush 20 mesh, be put into tank；
（2）, add 70% methanol of 15 times of stevia rebaudian leaf weight and the citric acid of 1.5 times of stevia rebaudian leaf weight is percolated 6 hours；
（3）, by after filtrate again micro- worry, cross resin decolorization；
（4）, after decolouring clear liquid again by the milipore filter ultrafiltration that molecular cut off is 10000,；
（5）, again pass through molecular cut off be 400 molecules NF membrane；
（6）, filter liquor be concentrated into paste, add methanol, methanol content is reached more than 90%；
（7）30 DEG C are warming up to, stirring stands 6 hours to there is crystallization to separate out；
（8）It is centrifuged, solid is dried after centrifugation, obtains thick steviol glycoside；
（9）In 90% ethanol that thick steviol glycoside is put into 5 times of steviol glycoside weight, then it is warming up to 70 degrees Celsius, stirring is to there is knot
Partial crystallization goes out, secondary to stand；
（10）Dried again after centrifugal filtration, obtain steviol glycoside.
The beneficial effects of the present invention are, 1, extract part eliminate pectin, color first with 70% methanol and citric acid
Element, plant polyose；2nd, advanced membrane separation technique has been employed many times and has removed the miscellaneous molecule of size；3rd, the purifying of resin adsorption is eliminated
Method；4th, the production cycle is shortened, is accomplished without any letup from blade to commerieal sugar is obtained；5th, the rate of output is improved, cost is reduced；6、
Using, without waste water, solving waste water pollution problem after the technique.
Embodiment
Embodiment 1
The mesh of stevia rebaudian leaf coarse crushing 20 is put into tank, 70% methanol and 1.5 times of stevia rebaudian leaf weight of 15 times of stevia rebaudian leaf weight is added
Citric acid is percolated, about 6 hours, and filtrate crosses resin decolorization, clear liquid crosses the molecule of ultrafiltration 10000, after 400 points of nanofiltration after micro-filtration
Son, filter liquor is concentrated into paste through film, adds methanol, content is reached 90%, is warming up to 30 degree, stirring to there is crystallization to separate out,
6 hours are stood, centrifugal filtration（Disposing mother liquor methanol obtains crude product）, steviol glycoside is obtained, is dried, plus 5 times of 90% ethanol, it is warming up to 70
Degree, stirring stands 6 hours, centrifugal filtration to there is crystallization to separate out（Disposing mother liquor ethanol obtains crude product）, through dry product stevioside
Product.
Claims (2)
1. a kind of extracting method of stevioside, it is characterised in that described method comprises the following steps：
（1）, crush stevia rebaudian leaf, be put into tank；
（2）, add 70% methanol and citric acid diafiltration；
（3）, by after filtrate again micro- worry, cross resin decolorization；
（4）, clear liquid ultrafiltration again after decolouring；
（5）, nanofiltration again；
（6）, filter liquor be concentrated into paste, add methanol, methanol content is reached more than 90%；
（7）, be warming up to 28-32 DEG C, stirring is stood to there is crystallization to separate out；
（8）, be centrifuged, after centrifugation solid dry, obtain thick steviol glycoside；
（9）, thick steviol glycoside is put into ethanol, then be warming up to 70 degrees Celsius, stirring is secondary to stand to there is crystallization to separate out；
（10）, again dry after centrifugal filtration, obtain steviol glycoside.
2. a kind of extracting method of stevioside, it is characterised in that described method comprises the following steps：
（1）, crush stevia rebaudian leaf crush 20 mesh, be put into tank；
（2）, add 70% methanol of 15 times of stevia rebaudian leaf weight and the citric acid of 1.5 times of stevia rebaudian leaf weight is percolated 6 hours；
（3）, by after filtrate again micro- worry, cross resin decolorization；
（4）, after decolouring clear liquid again by the milipore filter ultrafiltration that molecular cut off is 10000,；
（5）, again pass through molecular cut off be 400 molecules NF membrane；
（6）, filter liquor be concentrated into paste, add methanol, methanol content is reached more than 90%；
（7）, be warming up to 30 DEG C, stirring stands 6 hours to there is crystallization to separate out；
（8）, be centrifuged, after centrifugation solid dry, obtain thick steviol glycoside；
（9）, thick steviol glycoside is put into 90% ethanol of 5 times of steviol glycoside weight, then be warming up to 70 degrees Celsius, stirring is to having
Crystallization is separated out, secondary to stand；
（10）, again dry after centrifugal filtration, obtain steviol glycoside.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710307983.7A CN107163090A (en) | 2017-05-04 | 2017-05-04 | A kind of extracting method of stevioside |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710307983.7A CN107163090A (en) | 2017-05-04 | 2017-05-04 | A kind of extracting method of stevioside |
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| Publication Number | Publication Date |
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| CN107163090A true CN107163090A (en) | 2017-09-15 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201710307983.7A Pending CN107163090A (en) | 2017-05-04 | 2017-05-04 | A kind of extracting method of stevioside |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109354596A (en) * | 2018-10-09 | 2019-02-19 | 山东奥晶生物科技有限公司 | A kind of preparation method of efficient steviol glycoside |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102199178A (en) * | 2011-04-10 | 2011-09-28 | 胡玮 | Extraction process of rebaudioside A (RA) |
| CN102775452A (en) * | 2012-06-14 | 2012-11-14 | 甘肃普华甜菊糖开发有限公司 | Stevioside purification process |
| CN104530154A (en) * | 2014-12-23 | 2015-04-22 | 天津北洋百川生物技术有限公司 | Novel stevioside production process for reducing melanogenesis in process of soaking stevia rebaudian |
| CN104876982A (en) * | 2015-01-23 | 2015-09-02 | 南通海天生物科技有限公司 | Technology for separating stervioside from stevia rebaudian leaf |
-
2017
- 2017-05-04 CN CN201710307983.7A patent/CN107163090A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102199178A (en) * | 2011-04-10 | 2011-09-28 | 胡玮 | Extraction process of rebaudioside A (RA) |
| CN102775452A (en) * | 2012-06-14 | 2012-11-14 | 甘肃普华甜菊糖开发有限公司 | Stevioside purification process |
| CN104530154A (en) * | 2014-12-23 | 2015-04-22 | 天津北洋百川生物技术有限公司 | Novel stevioside production process for reducing melanogenesis in process of soaking stevia rebaudian |
| CN104876982A (en) * | 2015-01-23 | 2015-09-02 | 南通海天生物科技有限公司 | Technology for separating stervioside from stevia rebaudian leaf |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109354596A (en) * | 2018-10-09 | 2019-02-19 | 山东奥晶生物科技有限公司 | A kind of preparation method of efficient steviol glycoside |
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Application publication date: 20170915 |