CN107158462A - A kind of bioactivity glass composite biological tissues repair materials and preparation method thereof - Google Patents

A kind of bioactivity glass composite biological tissues repair materials and preparation method thereof Download PDF

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Publication number
CN107158462A
CN107158462A CN201710273286.4A CN201710273286A CN107158462A CN 107158462 A CN107158462 A CN 107158462A CN 201710273286 A CN201710273286 A CN 201710273286A CN 107158462 A CN107158462 A CN 107158462A
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bioactivity glass
solution
fibroin
repair materials
composite biological
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张春松
吴长应
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Wuhu Yangzhan New Material Technology Service Co Ltd
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Wuhu Yangzhan New Material Technology Service Co Ltd
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/3637—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the origin of the biological material other than human or animal, e.g. plant extracts, algae
    • A—HUMAN NECESSITIES
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    • A—HUMAN NECESSITIES
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    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
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    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/14—Macromolecular materials
    • A61L27/22—Polypeptides or derivatives thereof, e.g. degradation products
    • A61L27/227—Other specific proteins or polypeptides not covered by A61L27/222, A61L27/225 or A61L27/24
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A61L27/54—Biologically active materials, e.g. therapeutic substances
    • A—HUMAN NECESSITIES
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    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A61L27/56—Porous materials, e.g. foams or sponges
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    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
    • A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
    • A61L2300/204—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials with nitrogen-containing functional groups, e.g. aminoxides, nitriles, guanidines
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    • A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
    • A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
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    • A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
    • A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
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    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
    • A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
    • A61L2300/412—Tissue-regenerating or healing or proliferative agents

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Abstract

A kind of bioactivity glass composite biological tissues repair materials and preparation method thereof, belong to composite biological material field, are made up of the raw material of following mass percent：Bioactivity glass precursor liquid 35 45%, tetrahydrofuran 35 45%, fibroin solutions 5 10%, fibroin nanofiber solution 5 10%, aulin 2 3%, PGA 2 3%, green-tea extract 1 2%, Sodium Hyaluronate 1 2%, allantoin 1 2%；Bioactivity glass composite biological tissues repair materials produced by the present invention have good good mechanical property, and excellent medicinal tack.

Description

A kind of bioactivity glass composite biological tissues repair materials and preparation method thereof
Technical field
Material is repaired the invention belongs to composite biological material field, more particularly to a kind of bioactivity glass composite biological tissues Material and preparation method thereof.
Background technology
With the transition and upgrade of textile industry, the traditional textile using fibre as representative is just gradually to high-tech The weaving bio-medical material development of content.The medical textile of current China it is main with the health care of Nonimplantation material, defend Based on raw health care, protection class category and medical dressing class textile material, its technical merit is still in the junior stage, and external mistake The high-end Biomedical Textiles such as filter use and the imitative device class suitable for inside of human body almost rely on import, such as artificial kidney, the artificial heart Dirty, artificial lung, artificial bone, artificial skin etc..Found that so far, people's concern is more glass component to biology from 1971 The influence of activity.The rise of nanometer technology, makes people start to recognize that structure, to bioactivity also material impact, is more paid attention to The influence of dimensional effect, microcosmic fine structure to material structure and performance.From the nano biological of nano-scale R ＆ D design Glass, mesoporous bioglass are even more the concern for enjoying domestic and foreign scholars.Bio-vitric energy active cell gene, is conducive to enhancing thin The propagation of born of the same parents and differentiation, the effect with self-bone grafting and osteoacusis.But in view of the presence of amorphous silicon skeleton, bio-vitric is still in It is now amorphous state, bad mechanical property and drug attachment is poor.
The content of the invention
In order to solve problems of the prior art, the invention provides a kind of good mechanical property, and with excellent The bioactivity glass composite biological tissues repair materials of drug attachment.
The present invention uses following technical scheme：
A kind of bioactivity glass composite biological tissues repair materials, are made up of the raw material of following mass percent：It is biological Activity glass precursor liquid 35-45%, tetrahydrofuran 35-45%, fibroin solutions 5-10%, fibroin nanofiber solution 5-10%, aulin 2-3%, PGA 2-3%, green-tea extract 1-2%, Sodium Hyaluronate 1-2%, allantoin 1- 2%；
Described bioactivity glass precursor liquid is prepared using following methods：4g P123 are dissolved in 60g ethanol, fully After stirring, 6.7g silester, 0.73g triethyl phosphates, 1.4g Ca (NO are sequentially added3)2·4H2O, 1g 0.5M hydrochloric acid are molten Liquid；Mixed solution is stirred at room temperature after 24h and stood, when solution is in thick solution, that is, is made before bioactivity glass Drive liquid.
It is preferred that, described fibroin solutions are prepared using following methods：2L deionized waters are heated to boiling, added 4g sodium carbonate and 5g silkworm raw silks, boil after 30min, are cleaned up and dried with deionized water, then with 500ml, 9.3mol/L The dissolving of LiBr solution, solution is loaded into bag filter until completely dissolved, deionized water dialysis 72h, 9000r/min centrifugation 20min, is repeated twice, and obtains fibroin solutions.
It is preferred that, described fibroin nanofiber solution is prepared using following methods：Fibroin solutions are taken to be placed in Be concentrated into the half of original volume in 60 DEG C of insulating boxs, then be transferred to be concentrated into fume hood mass fraction be 20-30% concentrate, It is 0.5% concentrate to be put into after 4 DEG C of refrigerator storage 2d concentrate is diluted with water into mass fraction, is put after full and uniform stirring Enter and be incubated in insulating box to the fibroin nanofiber solution for forming translucent gels shape.
It is preferred that, described green-tea extract is prepared using following methods：Green tea 20-25g is weighed, its weight 10- is added 15 times of distilled water, decocts and extracts 1-1.5h, and suction filtration obtains filtrate 1；The distilled water of 8-10 times of filter residue weight is added into filter residue, is decocted Extraction 1-1.5h is boiled, suction filtration obtains filtrate 2；The distilled water of 8-10 times of filter residue weight is added into filter residue again, decocts and extracts 1-1.5h, Suction filtration obtains filtrate 3；Filtrate 1, filtrate 2, filtrate 3 are merged, are 40-50 DEG C, under the conditions of vacuum is 0.05-0.08Pa in temperature 4-6 times of green tea weight is concentrated under reduced pressure into, with three layers of filtered through gauze, green tea extractive liquor is produced.
A kind of preparation method of bioactivity glass composite biological tissues repair materials, comprises the following steps：Weigh respectively Bioactivity glass forerunner, fibroin solutions, fibroin nanofiber solution, aulin, PGA, green tea are extracted Thing, Sodium Hyaluronate and allantoin, are added in tetrahydrofuran, it are disperseed using ultrasonic oscillation, magnetic agitation 1h is molten Solution, and continue to stir 8h at room temperature, obtain viscous solution and be placed in the medical needle tubings of the 20ml being connected with high-pressure installation, stainless steel Syringe needle is fixed at receiver board 20cm, is set voltage and is carried out electrostatic spinning as 20k V, by obtained fiber at 40 DEG C Vacuum drying, obtains repair materials.
The beneficial effects of the present invention are：
1) there is higher porosity using bioactivity glass produced by the present invention, propagation, migration in order to cell, Enough nutriments are provided for the growth of cell.
2) fibroin biocompatibility is good, and degradation speed is slow and catabolite has low immunogenicity to tissue, Nutritious and repair ability is organized to skin, periodontal etc., when cultivating cell in vivo or in vitro, the fibroin albumen energy of loose structure Sticking, sprawl and breeding for cell is adapted to, the repair and reconstruction to tissue are extremely advantageous, are a kind of biological materials of excellent performance Material.Fibroin, PGA are added, the biocompatibility and physical and mechanical properties of material is added.
3) nanofiber is added, the nanofibrous structures on the pore-creating character of support, support hole wall is improved, considerably increases The specific surface area of support.
4) addition aulin, green-tea extract, Sodium Hyaluronate, allantoin, assign material certain pharmaceutical characteristic, carry High materials microstructure repair ability.
Embodiment
Embodiment 1
A kind of bioactivity glass composite biological tissues repair materials, are made up of the raw material of following mass percent：It is biological Activity glass precursor liquid 35%, tetrahydrofuran 35%, fibroin solutions 10%, fibroin nanofiber solution 10%, Buddhist nun Mei Shuli 3%, PGA 3%, green-tea extract 2%, Sodium Hyaluronate 1%, allantoin 1%.
Described bioactivity glass precursor liquid is prepared using following methods：4g P123 are dissolved in 60g ethanol, fully After stirring, 6.7g silester, 0.73g triethyl phosphates, 1.4g Ca (NO are sequentially added3)2·4H2O, 1g 0.5M hydrochloric acid are molten Liquid；Mixed solution is stirred at room temperature after 24h and stood, when solution is in thick solution, that is, is made before bioactivity glass Drive liquid.
Described fibroin solutions are prepared using following methods：2L deionized waters are heated to boiling, 4g carbonic acid is added Sodium and 5g silkworm raw silks, boil after 30min, are cleaned up and dried with deionized water, then with 500ml, 9.3mol/L LiBr Solution is dissolved, and solution is loaded into bag filter until completely dissolved, and deionized water dialysis 72h, 9000r/min centrifugation 20min is repeated Twice, fibroin solutions are obtained.
Described fibroin nanofiber solution is prepared using following methods：Fibroin solutions are taken to be placed in 60 DEG C of constant temperature The half of original volume is concentrated into case, then is transferred to the concentrate for being concentrated into that mass fraction is 20-30% in fume hood, by concentrate It is 0.5% to be put into after 4 DEG C of refrigerator storage 2d concentrate being diluted with water mass fraction, and insulating box is put into after full and uniform stirring Interior incubation extremely forms the fibroin nanofiber solution of translucent gels shape.
Described green-tea extract is prepared using following methods：Green tea 20g is weighed, the distilled water of 10 times of its weight is added, Decoct and extract 1h, suction filtration obtains filtrate 1；The distilled water of 8 times of filter residue weight is added into filter residue, decocts and extracts 1h, suction filtration obtains filtrate 2；The distilled water of 8 times of filter residue weight is added into filter residue again, decocts and extracts 1h, suction filtration obtains filtrate 3；By filtrate 1, filtrate 2, filtrate 3 merge, temperature be 4 DEG C, vacuum be 4-6 times that green tea weight is concentrated under reduced pressure under the conditions of 0.05Pa, with three layers of gauze mistake Filter, produces green tea extractive liquor.
A kind of preparation method of bioactivity glass composite biological tissues repair materials, comprises the following steps：Weigh respectively Bioactivity glass forerunner, fibroin solutions, fibroin nanofiber solution, aulin, PGA, green tea are extracted Thing, Sodium Hyaluronate and allantoin, are added in tetrahydrofuran, it are disperseed using ultrasonic oscillation, magnetic agitation 1h is molten Solution, and continue to stir 8h at room temperature, obtain viscous solution and be placed in the medical needle tubings of the 20ml being connected with high-pressure installation, stainless steel Syringe needle is fixed at receiver board 20cm, is set voltage and is carried out electrostatic spinning as 20k V, by obtained fiber at 40 DEG C Vacuum drying, obtains repair materials.
Embodiment 2
A kind of bioactivity glass composite biological tissues repair materials, are made up of the raw material of following mass percent：It is biological Activity glass precursor liquid 45%, tetrahydrofuran 35%, fibroin solutions 5%, fibroin nanofiber solution 5%, Ni Mei Shu Li 3%, PGA 3%, green-tea extract 2%, Sodium Hyaluronate 1%, allantoin 1%.
Described bioactivity glass precursor liquid is prepared using following methods：4g P123 are dissolved in 60g ethanol, fully After stirring, 6.7g silester, 0.73g triethyl phosphates, 1.4g Ca (NO are sequentially added3)2·4H2O, 1g 0.5M hydrochloric acid are molten Liquid；Mixed solution is stirred at room temperature after 24h and stood, when solution is in thick solution, that is, is made before bioactivity glass Drive liquid.
Described fibroin solutions are prepared using following methods：2L deionized waters are heated to boiling, 4g carbonic acid is added Sodium and 5g silkworm raw silks, boil after 30min, are cleaned up and dried with deionized water, then with 500ml, 9.3mol/L LiBr Solution is dissolved, and solution is loaded into bag filter until completely dissolved, and deionized water dialysis 72h, 9000r/min centrifugation 20min is repeated Twice, fibroin solutions are obtained.
Described fibroin nanofiber solution is prepared using following methods：Fibroin solutions are taken to be placed in 60 DEG C of constant temperature The half of original volume is concentrated into case, then is transferred to the concentrate for being concentrated into that mass fraction is 20-30% in fume hood, by concentrate It is 0.5% to be put into after 4 DEG C of refrigerator storage 2d concentrate being diluted with water mass fraction, and insulating box is put into after full and uniform stirring Interior incubation extremely forms the fibroin nanofiber solution of translucent gels shape.
Described green-tea extract is prepared using following methods：Green tea 25g is weighed, the distilled water of 15 times of its weight is added, Decoct and extract 1.5h, suction filtration obtains filtrate 1；The distilled water of 10 times of filter residue weight is added into filter residue, decocts and extracts 1.5h, suction filtration is obtained Filtrate 2；The distilled water of 10 times of filter residue weight is added into filter residue again, decocts and extracts 1.5h, suction filtration obtains filtrate 3；By filtrate 1, filter Liquid 2, filtrate 3 merge, temperature be 50 DEG C, vacuum be 6 times that green tea weight is concentrated under reduced pressure under the conditions of 0.08Pa, with three layers Filtered through gauze, produces green tea extractive liquor.
A kind of preparation method of bioactivity glass composite biological tissues repair materials, comprises the following steps：Weigh respectively Bioactivity glass forerunner, fibroin solutions, fibroin nanofiber solution, aulin, PGA, green tea are extracted Thing, Sodium Hyaluronate and allantoin, are added in tetrahydrofuran, it are disperseed using ultrasonic oscillation, magnetic agitation 1h is molten Solution, and continue to stir 8h at room temperature, obtain viscous solution and be placed in the medical needle tubings of the 20ml being connected with high-pressure installation, stainless steel Syringe needle is fixed at receiver board 20cm, is set voltage and is carried out electrostatic spinning as 20k V, by obtained fiber at 40 DEG C Vacuum drying, obtains repair materials.
At room temperature, with material made from SLBI-500N types electronic tension tester measure embodiment 1, embodiment 2 in dry state Under tensile strength and contrasted with the tensile strength of pure bioactive glass material, as a result see the table below 1.
The tensile strength of table 1
Embodiment 1 Embodiment 2 Pure bioactive glass material
65MPa 66MPa 59MPa
As a result：The tensile strength of tissue renovation material made from embodiment 1, embodiment 2 is apparently higher than pure bioactive glass The tensile strength of glass material.
12 small white mouses are selected, 3 groups is randomly divided into, every group 4,12 × 2cm is produced on the backbone of every white mouse Full thickness dermal wound model, blank control group and two experimental groups (embodiment 1, embodiment 2) are divided into again.In experiment Observe the general condition and healing time of wound healing daily afterwards；Simultaneously wound tissue, formalin solution are taken at surface of a wound center It is fixed, FFPE, soft tissue slices, row HE dyeing, observes blank control group under the microscope and experimental group skin holostrome lacks The healing state in damage face.7th, 14,21,28, the surface of a wound photograph respectively to experimental group and blank control group in 35 days adopt figure, use tricks Calculation machine image analysis software calculates the wound healing area of each group, determines the wound healing percentage of each group, is cured by the formula surface of a wound Conjunction rate (%)=[(surface of a wound original area-surface of a wound do not heal area)/surface of a wound original area] × 100%, as a result see the table below 2.
The each group Wound healing rate of table 2 compares
As a result：The wound healing time of experimental group is respectively：Embodiment 1 is that 23d, embodiment 2 are 22d, and blank group Healing time is 29d；Understood in contrast blank control group and the healing time and Wound healing rate that apply the experimental group for spilling material：Wound Face ordinary circumstance experimental group is significantly better than blank control group, and the repair materials used in experimental group are cured to full thickness dermal with rush Cooperation is used.

Claims (7)

1. a kind of bioactivity glass composite biological tissues repair materials, it is characterised in that by the raw material of following mass percent Constitute：Bioactivity glass precursor liquid 35-45%, tetrahydrofuran 35-45%, fibroin solutions 5-10%, fibroin is received Rice fiber solution 5-10%, aulin 2-3%, PGA 2-3%, green-tea extract 1-2%, Sodium Hyaluronate 1-2%, Allantoin 1-2%；
Described bioactivity glass precursor liquid is prepared using following methods：4g P123 are dissolved in 60g ethanol, are sufficiently stirred for Afterwards, 6.7g silester, 0.73g triethyl phosphates, 1.4g Ca (NO are sequentially added3)2·4H2O, 1g 0.5M hydrochloric acid solutions；Will Mixed solution is stirred at room temperature after 24h and stood, and when solution is in thick solution, that is, bioactivity glass precursor liquid is made.
2. a kind of bioactivity glass composite biological tissues repair materials according to claim 1, it is characterised in that described Fibroin solutions using following methods prepare：2L deionized waters are heated to boiling, 4g sodium carbonate and the life of 5g silkworms is added Silk, boils after 30min, is cleaned up and dried with deionized water, then is dissolved with 500ml, 9.3mol/L LiBr solution, has treated Solution is loaded into bag filter after fully dissolved, deionized water dialysis 72h, 9000r/min centrifugation 20min is repeated twice, obtains silk egg White solution.
3. a kind of bioactivity glass composite biological tissues repair materials according to claim 1, it is characterised in that described Fibroin nanofiber solution using following methods prepare：Take fibroin solutions to be placed in 60 DEG C of insulating boxs and be concentrated into original The half of volume, then the concentrate for being concentrated into that mass fraction is 20-30% in fume hood is transferred to, concentrate is put into 4 DEG C of refrigerators It is 0.5% that concentrate is diluted with water into mass fraction after storage 2d, is put into insulating box and is incubated to shape after full and uniform stirring Into the fibroin nanofiber solution of translucent gels shape.
4. a kind of bioactivity glass composite biological tissues repair materials according to claim 1, it is characterised in that by with The raw material of lower mass percent is constituted：Bioactivity glass precursor liquid 35%, tetrahydrofuran 35%, fibroin solutions 10%, Fibroin nanofiber solution 10%, aulin 3%, PGA 3%, green-tea extract 2%, Sodium Hyaluronate 1%, Allantoin 1%.
5. a kind of bioactivity glass composite biological tissues repair materials according to claim 1, it is characterised in that by with The raw material of lower mass percent is constituted：Bioactivity glass precursor liquid 45%, tetrahydrofuran 35%, fibroin solutions 5%, silkworm Silk-fibroin nanofiber solution 5%, aulin 3%, PGA 3%, green-tea extract 2%, Sodium Hyaluronate 1%, urine Bursin 1%.
6. a kind of bioactivity glass composite biological tissues repair materials according to claim 1, it is characterised in that described Green-tea extract using following methods prepare：Green tea 20-25g is weighed, the distilled water of 10-15 times of its weight is added, decoction is carried 1-1.5h is taken, suction filtration obtains filtrate 1；The distilled water of 8-10 times of filter residue weight is added into filter residue, decocts and extracts 1-1.5h, suction filtration is obtained Filtrate 2；The distilled water of 8-10 times of filter residue weight is added into filter residue again, decocts and extracts 1-1.5h, suction filtration obtains filtrate 3；By filtrate 1st, filtrate 2, filtrate 3 merge, temperature be 40-50 DEG C, vacuum be to be concentrated under reduced pressure into green tea weight under the conditions of 0.05-0.08Pa 4-6 times, with three layers of filtered through gauze, produce green tea extractive liquor.
7. a kind of preparation side of bioactivity glass composite biological tissues repair materials as described in claim any one of 1-6 Method, comprises the following steps：Bioactivity glass forerunner, fibroin solutions, fibroin nanofiber solution, Buddhist nun are weighed respectively Mei Shuli, PGA, green-tea extract, Sodium Hyaluronate and allantoin, add in tetrahydrofuran, use ultrasonic oscillation pair It is disperseed, magnetic agitation 1h dissolvings, and continues to stir 8h at room temperature, obtains viscous solution and be placed in be connected with high-pressure installation The medical needle tubings of 20ml in, stainless steel syringe needle is fixed at receiver board 20cm, set voltage as 20kV carry out electrostatic spinning, Obtained fiber is dried in vacuo at 40 DEG C, repair materials are obtained.
CN201710273286.4A 2017-04-21 2017-04-21 A kind of bioactivity glass composite biological tissues repair materials and preparation method thereof Pending CN107158462A (en)

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Application publication date: 20170915