CN107156105A - A kind of small insects external genital organs persistence method - Google Patents
A kind of small insects external genital organs persistence method Download PDFInfo
- Publication number
- CN107156105A CN107156105A CN201710393399.8A CN201710393399A CN107156105A CN 107156105 A CN107156105 A CN 107156105A CN 201710393399 A CN201710393399 A CN 201710393399A CN 107156105 A CN107156105 A CN 107156105A
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- China
- Prior art keywords
- genital organs
- external genital
- insect
- ethyl alcohol
- small insects
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention belongs to insect specimen Techniques of preserving field, a kind of small insects external genital organs persistence method is disclosed, the small insects external genital organs persistence method comprises the following steps：The fresh specimens of collection are placed in the test tube of absolute ethyl alcohol and take back laboratory；It is put into 35~40 DEG C of low temperature refrigerators and stores；Identification species sample is taken out, removed from belly, dissection, by Each part observation finish after be put into absolute ethyl alcohol rinse 34 times, by excessive moisture dilute even remove, be put into the clear glass device for have triglycerides preserve.The present invention solves dead volume insect specimen and easily rotted so that insect specimen is long-term or forever well preserves；And some important features can be checked with repeated multiple times taking-up.
Description
Technical field
The invention belongs to insect specimen Techniques of preserving field, more particularly to a kind of small insects external genital organs persistence side
Method.
Background technology
Current most people protects insect specimen using camphor ball expelling parasite, and this method needs whole preparation of specimen
To be placed in Riker mount, waste time and energy, and space-consuming is very big.If once whole preparation of specimen deposit into exsiccata, it
Easily gnawed by booklice etc., while will largely lose the integrality of internal gene order, it is impossible to as follow-up point
Gene extraction of son experiment etc..Persistence method can not only be such that insect specimen is forever deposited, and this method may be used also
To save parking space, and the extraction of molecule experiments gene is used directly for, prior art is by insect external genital organs etc.
Vital classification tissue (structure) is fabricated to disposable slide using canada balsam bonding.Having the disadvantage cannot be anti-Yi Dan be molded
Check sees that certain structures can only see the state of a certain angle of a certain structure, and can not show the overall picture of the structure.
In summary, the problem of prior art is present be：Prior art makes the vital classification tissue such as insect external genital organs
Disposable slide is fabricated to canada balsam bonding to check repeatedly, it is impossible to show the important shape of structure from different perspectives
Properties and characteristicses.
The content of the invention
The problem of existing for prior art, the invention provides a kind of small insects external genital organs persistence method.
The present invention is achieved in that a kind of small insects external genital organs persistence method, and the small insects are external
Device persistence method is grown to comprise the following steps：
Step one, the fresh specimens of collection are placed in absolute ethyl alcohol test tube；
Step 2, is put into -35 DEG C~-40 DEG C low temperature refrigerators and preserves；
Step 3, takes out sample from absolute ethyl alcohol, is connected with insect needle or thin blade from insect chest with belly
Joint removes the whole belly of insect, and body surface excessive moisture is removed with blotting paper, is put into KOH solution and stands；
Step 4, the insect afterbody for being handled de- meat with pincet takes out from KOH solution, is put into absolute ethyl alcohol and floats
Wash；
Step 5, is dissected its external genital organs under Stereo microscope using special insect anatomy pin, and each several part is special
Levy structure to peel off one by one, microscopy observation is carried out under the microscope；
Step 6, genitals are put into glycerine：Clear water=7:Observed in 3 solution；Observation is put into absolute ethyl alcohol after finishing
Rinsing；
Step 7, is put into sealed glassware, while the CaO for being put into 10g stands 2-3 days as drier；
Step 8, is put into clear glass straight tube and preserves, bottom gauze wrapped 5~8g sorbic acid preservative particles, on
Portion is put into triglyceride with glue head dropper, can persistence with rubber stopper seal.
Further, concentration is put into the step 3 to stand -15 hours 12 hours in 10%~15%KOH solution.
Further, it is put into absolute ethyl alcohol and rinses 3 times -4 times in the step 6.
Further, glass straight tube diameter 0.5mm-1.0mm in the step 8；Triglyceride is put into up to glass container
2/3 height.
Advantages of the present invention and good effect are：Solve dead volume insect specimen easily to rot so that insect specimen
Long-term or permanent good preservation；It can be checked with repeated multiple times taking-up, each tissue structural form feature not influenceed；With existing method phase
Than can under equal conditions save 8-10 times of storage area.
Brief description of the drawings
Fig. 1 is small insects external genital organs persistence method flow diagram provided in an embodiment of the present invention.
Embodiment
In order to make the purpose , technical scheme and advantage of the present invention be clearer, with reference to embodiments, to the present invention
It is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, it is not used to
Limit the present invention.
The application principle of the present invention is explained in detail below in conjunction with the accompanying drawings.
As shown in figure 1, small insects external genital organs persistence method provided in an embodiment of the present invention comprises the following steps：
S101：The fresh specimens (insect condition of living organism) of collection are placed in the test tube of absolute ethyl alcohol (100%) at once to be taken back
Laboratory；
S102：It is put into -35 DEG C~-40 DEG C low temperature refrigerators and preserves；
S103：Sample is taken out from absolute ethyl alcohol, the pass being connected with insect needle or thin blade from insect chest with belly
The whole belly of insect is removed at section, body surface excessive moisture is removed with blotting paper, concentration is put into be quiet in 10~15%KOH solution
Put 12-15 hours, to remove muscle unnecessary in structure unless each, be only left bony structures, make external genital organs Each part more
Clearly, it is easy to next step anatomic observation；
S104：The insect afterbody for being handled de- meat with pincet takes out from KOH solution, and rinses in absolute ethyl alcohol 1-
2 times；
S105：Its external genital organs is dissected under Stereo microscope using special insect anatomy pin, and by each several part feature
Structure is peeled off one by one, and microscopy observation is carried out under the microscope；
S106：During observation, genitals are put into glycerine：Clear water=7:Observed in 3 solution；Observation is put into anhydrous after finishing
Rinsed 3-4 times in ethanol；
S107：It is then placed in sealed glassware, while the CaO for being put into 10g or so stands 2-3 as drier
My god, to remove remaining moisture in structure；
S108：It is put into clear glass straight tube and preserves, the glass tube is unsuitable excessive for small insects, and general diameter exists
0.5-1.0mm is advisable, bottom flat, and non-arc can be kept flat.Medicine is successively：Bottom gauze wrapped 5~8g sorbic acid anti-corrosions
Agent particle, the triglyceride just broken a seal is put into glue head dropper for top until 2/3 height of glass container, uses rubber stopper seal
Can persistence.
The present invention solves dead volume insect specimen and easily rotted so that insect specimen is long-term or forever well preserves；
It can be checked with repeated multiple times taking-up, each tissue structural form feature not influenceed；Compared with the conventional method under equal conditions can be with
The storage area of 8-10 times of saving.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention
Any modifications, equivalent substitutions and improvements made within refreshing and principle etc., should be included in the scope of the protection.
Claims (4)
1. a kind of small insects external genital organs persistence method, it is characterised in that the small insects external genital organs is forever protected
The method of depositing comprises the following steps：
Step one, the fresh specimens of collection are placed in absolute ethyl alcohol test tube；
Step 2, is put into -35 DEG C~-40 DEG C low temperature refrigerators and preserves；
Step 3, takes out sample from absolute ethyl alcohol, the joint being connected with insect needle or thin blade from insect chest with belly
The whole belly of insect is removed at place, and body surface excessive moisture is removed with blotting paper, is put into KOH solution and is stood；
Step 4, the insect afterbody for being handled de- meat with pincet takes out from KOH solution, is put into absolute ethyl alcohol and rinses；
Step 5, is dissected its external genital organs under Stereo microscope using special insect anatomy pin, and by each several part feature knot
Structure is peeled off one by one, and microscopy observation is carried out under the microscope；
Step 6, genitals are put into glycerine：Clear water=7:Observed in 3 solution；Observation is put into absolute ethyl alcohol after finishing and rinsed；
Step 7, is put into sealed glassware, while the CaO for being put into 10g stands 2-3 days as drier；
Step 8, is put into clear glass straight tube and preserves, and bottom gauze wrapped 5~8g sorbic acid preservative particles, top is used
Glue head dropper is put into triglyceride, can persistence with rubber stopper seal.
2. small insects external genital organs persistence method as claimed in claim 1, it is characterised in that put in the step 4
Enter concentration to stand -15 hours 12 hours in 10%~15%KOH solution.
3. small insects external genital organs persistence method as claimed in claim 1, it is characterised in that put in the step 5
Enter in absolute ethyl alcohol and rinse 3 times -4 times.
4. small insects external genital organs persistence method as claimed in claim 1, it is characterised in that glass in the step 7
Glass straight tube diameter 0.5mm-1.0mm；Triglyceride is put into until 2/3 height of glass container.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710393399.8A CN107156105A (en) | 2017-05-27 | 2017-05-27 | A kind of small insects external genital organs persistence method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710393399.8A CN107156105A (en) | 2017-05-27 | 2017-05-27 | A kind of small insects external genital organs persistence method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107156105A true CN107156105A (en) | 2017-09-15 |
Family
ID=59821042
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710393399.8A Pending CN107156105A (en) | 2017-05-27 | 2017-05-27 | A kind of small insects external genital organs persistence method |
Country Status (1)
| Country | Link |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111480643A (en) * | 2020-06-01 | 2020-08-04 | 中国科学院昆明动物研究所 | Insect specimen manufacturing method |
| CN114246175A (en) * | 2021-04-25 | 2022-03-29 | 华南农业大学 | Method for researching male genitalia of coleoptera insect |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH10109901A (en) * | 1996-10-02 | 1998-04-28 | King Kogyo Kk | Sample cabinet |
| CN204317364U (en) * | 2014-12-11 | 2015-05-13 | 贵州大学 | A kind of device preserving insect genitalia |
-
2017
- 2017-05-27 CN CN201710393399.8A patent/CN107156105A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH10109901A (en) * | 1996-10-02 | 1998-04-28 | King Kogyo Kk | Sample cabinet |
| CN204317364U (en) * | 2014-12-11 | 2015-05-13 | 贵州大学 | A kind of device preserving insect genitalia |
Non-Patent Citations (2)
| Title |
|---|
| 洪泽源等: "小蠹雄性生殖器玻片标本制作技巧", 《安徽农业科学》 * |
| 郑明顺等编著: "《生物标本技术》", 31 July 2005, 东北林业大学出版社 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111480643A (en) * | 2020-06-01 | 2020-08-04 | 中国科学院昆明动物研究所 | Insect specimen manufacturing method |
| CN114246175A (en) * | 2021-04-25 | 2022-03-29 | 华南农业大学 | Method for researching male genitalia of coleoptera insect |
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