CN107155666A - A kind of method of eggplant resistance to overhead flooding injury Rapid identification - Google Patents
A kind of method of eggplant resistance to overhead flooding injury Rapid identification Download PDFInfo
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- 235000002597 Solanum melongena Nutrition 0.000 title claims abstract description 81
- 244000061458 Solanum melongena Species 0.000 title claims abstract description 81
- 238000000034 method Methods 0.000 title claims abstract description 29
- 230000006378 damage Effects 0.000 title abstract description 9
- 208000027418 Wounds and injury Diseases 0.000 title abstract 7
- 208000014674 injury Diseases 0.000 title abstract 7
- 239000000463 material Substances 0.000 claims abstract description 64
- 238000011156 evaluation Methods 0.000 claims abstract description 28
- 241000196324 Embryophyta Species 0.000 claims abstract description 9
- 230000006578 abscission Effects 0.000 claims description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 11
- 238000011084 recovery Methods 0.000 claims description 9
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- 239000004568 cement Substances 0.000 claims description 6
- 230000002159 abnormal effect Effects 0.000 claims description 4
- 238000004299 exfoliation Methods 0.000 claims description 4
- 230000017074 necrotic cell death Effects 0.000 claims description 4
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- 230000035772 mutation Effects 0.000 abstract 1
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- GVJHHUAWPYXKBD-UHFFFAOYSA-N (±)-α-Tocopherol Chemical compound OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
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- 238000009331 sowing Methods 0.000 description 2
- 208000024891 symptom Diseases 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 1
- 229930003427 Vitamin E Natural products 0.000 description 1
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- 229930013930 alkaloid Natural products 0.000 description 1
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Abstract
The invention discloses a kind of method of eggplant resistance to overhead flooding injury Rapid identification, pass through seedling stage character mutation, by eggplant resistance to overhead flooding injury be divided into by force, in, weak 3 grades, using leaf color and deciduous, culm morphology the two evaluation indexes, by its quantitative classification, formulate ranking score standard, its average membership function value is calculated according to the average of each material, overall merit is carried out to resistance to overhead flooding injury, set up appraisement system, resistance to overhead flooding injury membership function value is the resistance to overhead flooding injury that plant is evaluated with overall target, its evaluation result with it is actual closer to.The present invention simple, quick, accurate by the average membership function value of damage or crop failure caused by waterlogging form score can identify eggplant resistance to overhead flooding injury.
Description
Technical Field
The invention relates to an eggplant waterlogging tolerance identification method, in particular to a method for quickly identifying the eggplant waterlogging tolerance.
Background
Eggplant (Solanum melongena L.) is one of ten vegetable crops in the world, and is cultivated in most regions of the world, particularly in asia, europe, africa, north america and the like. China is the largest eggplant producing country in the world and is also the secondary origin center of eggplants, and the cultivation area of China accounts for about 50 percent of the world. In 2013, the planting area of the eggplants in China is 80.1 ten thousand hectares, and the total yield reaches 2845.58 ten thousand tons. The eggplant has high nutritive value and is rich in vitamins and minerals, particularly the vitamin E, P and the content of iron element are high. In addition, the eggplant contains various alkaloids, and has wide medicinal value.
Eggplants planted in middle and lower reaches of Yangtze river in China are usually subjected to waterlogging due to excessive rainfall and high soil water content in seedling stage, so that great yield reduction is caused. Measures such as soil improvement, ditching and drainage are adopted, so that the method plays a certain role in reducing waterlogging damage, but the method is probably a more effective way for improving the waterlogging resistance of the eggplant in the seedling stage through genetic improvement. Therefore, the rapid identification and research on the waterlogging tolerance of the eggplant germplasm resources is carried out, and the method has important significance for screening a large number of germplasm resources and breeding the waterlogging tolerance of the eggplant.
Disclosure of Invention
The invention aims to solve the technical problem of providing a method for quickly identifying the waterlogging tolerance of eggplants.
In order to solve the technical problems, the technical scheme adopted by the invention is as follows:
a method for rapidly identifying waterlogging tolerance of eggplants is characterized by comprising the following steps:
the method comprises the following steps: performing flooding treatment on the four-leaf and one-heart eggplant seedlings, and performing flooding for 14 days after 2cm of soil surface is flooded;
step two: carrying out waterlogging recovery treatment after flooding for 14 days, carrying out eggplant seedling phenotype observation after recovering for 7 days, and selecting 2 evaluation indexes of leaf color, abscission property and stem form for evaluation;
step three: quantitative grading is carried out by adopting 2 evaluation indexes of leaf color, abscission property and stem shape, and a grade scoring standard is formulated;
step four: evaluating the grade score of the eggplant seedlings in each material by using the grade score standard, and calculating the average score of each material;
step five: and calculating the waterlogging tolerance membership function value by using the calculated average score of each material, accumulating and calculating the average number of the 2 waterlogging tolerance membership function values of each material to obtain the average membership function value of the material, comparing the waterlogging tolerance of different eggplant materials by using the average membership function value, and if the comprehensive membership function value is higher, indicating that the waterlogging tolerance of the eggplant is stronger.
Further, the first step specifically comprises the steps of sowing 30 seeds in each material in a hole tray, thinning and transplanting when the eggplant seedlings grow to two leaves and one heart, selecting 16 seedlings with the same growth vigor from each material, performing flooding treatment when the eggplant seedlings grow to four leaves and one heart, putting the hole tray in a cement flooding pool for flooding treatment, putting water in the pool to 2cm above the surface of soil, keeping the water level constant during flooding, and performing flooding treatment for 14 d.
And further, after the second step is specifically carried out, after the waterlogging treatment is carried out for 14d, all water in the cement flooded waterlogging pond is drained, normal growth management is carried out on the eggplant seedlings, and after 7d, grade score statistics is carried out on 2 evaluation indexes of leaf color and abscission performance of the eggplant seedlings and stem shapes.
Further, the evaluation indexes and the score standards of the waterlogging tolerance form of the eggplant seedlings in the third step are that the 2 evaluation indexes of the leaf color, the abscission property and the stem form are adopted for quantitative grading, and the grade score standard is formulated:
(1) leaf color and exfoliation:
a level: the blade basically recovers growth, or only the blade tip has withered and yellow phenomena, and the score is 0;
b stage: no withered leaves, yellow leaves are less than or equal to 2 leaves, and the score is 1;
c level: the seedlings return to normal growth, withered leaves do not exceed 3 leaves, and the score is 2;
d stage: most leaves of seedlings almost die, only heart leaves are yellow green, and the score is 3;
e, grade: all the leaves of the seedlings die, no green leaves exist, and the score is 4;
(2) the shape of the stem at the flooded position:
a level: the stalks grow normally at the flooded position without any abnormal growth phenomenon, and the score is 0;
b stage: the stem at the flooded position has the phenomenon of expansion and growth, and the score is 1;
c level: the stem at the flooded position has a shrinkage phenomenon with a tissue necrosis trend, and the score is 2;
d stage: the stalks at the flooded place are necrotic, the plants are lodging, and the score is 3.
Further, the average score of each material in the fourth step is calculated according to the formula:
in the formula, XiExpressing the number of plants in each waterlogging grade; n is a radical ofiAnd expressing the scores corresponding to the waterlogging grades.
Further, the flood tolerance membership function value X in the fifth stepijThe fuzzy mathematics membership function value method is used, and the calculation formula is as follows:
wherein,cold resistance membership function value, X, of j-th index of i-th typeijNumerical value, X, of the j-th index of the i-th typejmaxDenotes the maximum value of the j index, XjminA minimum value representing the j index;
if a certain index is negatively related to waterlogging tolerance, the waterlogging tolerance membership degree can be calculated by using an inverse membership function, and a formula is calculated:
wherein, if X is more than or equal to 0ijLess than or equal to 0.35, the material is a material with weaker waterlogging resistance; if 0.35 < XijThe waterlogging tolerance is equal to or less than 0.65, and the material is a material with moderate waterlogging tolerance; if 0.65 < XijLess than or equal to 1.0, the material is a material with stronger waterlogging resistance.
Compared with the prior art, the invention has the following advantages and effects: the invention provides basic experimental basis and experimental method for evaluating the waterlogging tolerance of eggplant germplasm resources, researching the waterlogging tolerance mechanism and breeding the waterlogging tolerance by researching the waterlogging morphological symptoms of the eggplant aiming at the current situation of weak foundation of the waterlogging tolerance research. The invention establishes the eggplant waterlogging tolerance evaluation system through visual morphological differences, can quickly screen out waterlogging tolerance germplasm resources from morphology, has simple and easy operation, objectively reflects the true condition of eggplant waterlogging, is a set of systematic method for researching the true condition of eggplant waterlogging at present, screens out waterlogging tolerance germplasm resources with strong waterlogging tolerance, is a batch of precious materials, and can be used for eggplant waterlogging tolerance breeding.
Drawings
Fig. 1 is a diagram illustrating the leaf waterlogging phenotype grade of an eggplant after flooding according to an embodiment of the invention.
Fig. 2 is a diagram illustrating the phenotype level of the waterlogged stalks after the eggplant is flooded with water according to the embodiment of the invention.
Detailed Description
The present invention will be described in further detail below by way of examples with reference to the accompanying drawings, which are illustrative of the present invention and are not to be construed as limiting the present invention.
The invention discloses a method for rapidly identifying waterlogging tolerance of eggplants, which is characterized by comprising the following steps:
the method comprises the following steps: sowing 30 seeds in each material in a hole tray, thinning and transplanting when the eggplant seedlings grow to two leaves and one heart, selecting 16 seedlings with the same growth vigor from each material, performing flooding treatment when the eggplant seedlings grow to four leaves and one heart, putting the hole tray into a cement flooding pool for flooding treatment, putting water in the pool to 2cm above the soil surface, keeping the water level constant during flooding, and performing flooding treatment for 14 d.
Step two: and after the waterlogging treatment is carried out for 14 days, completely draining water in the cement flooded waterlogging pond, carrying out normal growth management on the eggplant seedlings, and carrying out grade score statistics on 2 evaluation indexes of leaf color, abscission performance and stem morphology of the eggplant seedlings after 7 days.
Step three: quantitative grading is carried out by adopting 2 evaluation indexes of leaf color, abscission property and stem shape, and a grade scoring standard is formulated;
quantitative grading is carried out by adopting 2 evaluation indexes of leaf color, abscission property and stem shape, and a grade scoring standard is formulated:
(1) as shown in fig. 1, blade color and exfoliation:
a level: the blade basically recovers growth, or only the blade tip has withered and yellow phenomena, and the score is 0;
b stage: no withered leaves, yellow leaves are less than or equal to 2 leaves, and the score is 1;
c level: the seedlings return to normal growth, withered leaves do not exceed 3 leaves, and the score is 2;
d stage: most leaves of seedlings almost die, only heart leaves are yellow green, and the score is 3;
e, grade: all the leaves of the seedlings die, no green leaves exist, and the score is 4;
(2) as shown in fig. 2, the shape of the stem at the flooded position is as follows:
a level: the stalks grow normally at the flooded position without any abnormal growth phenomenon, and the score is 0;
b stage: the stem at the flooded position has the phenomenon of expansion and growth, and the score is 1;
c level: the stem at the flooded position has a shrinkage phenomenon with a tissue necrosis trend, and the score is 2;
d stage: the stalks at the flooded place are necrotic, the plants are lodging, and the score is 3.
Step four: evaluating the grade score of the eggplant seedlings in each material by using the grade score standard, and calculating the average score of each material;
the average score of each material is calculated according to the formula:
in the formula, XiExpressing the number of plants in each waterlogging grade; n is a radical ofiAnd expressing the scores corresponding to the waterlogging grades.
Step five: and calculating the waterlogging tolerance membership function value by using the calculated average score of each material, accumulating and calculating the average number of the 2 waterlogging tolerance membership function values of each material to obtain the average membership function value of the material, comparing the waterlogging tolerance of different eggplant materials by using the average membership function value, and if the comprehensive membership function value is higher, indicating that the waterlogging tolerance of the eggplant is stronger.
Waterlogging tolerance membership function value XijThe fuzzy mathematics membership function value method is used, and the calculation formula is as follows:
wherein,represents the ithj index cold tolerance membership function value, XijNumerical value, X, of the j-th index of the i-th typejmaxDenotes the maximum value of the j index, XjminA minimum value representing the j index;
if a certain index is negatively related to waterlogging tolerance, the waterlogging tolerance membership degree can be calculated by using an inverse membership function, and a formula is calculated:
wherein, if X is more than or equal to 0ijLess than or equal to 0.35, the material is a material with weaker waterlogging resistance; if 0.35 < XijThe waterlogging tolerance is equal to or less than 0.65, and the material is a material with moderate waterlogging tolerance; if 0.65 < XijLess than or equal to 1.0, the material is a material with stronger waterlogging resistance.
The invention provides basic experimental basis and experimental method for evaluating the waterlogging tolerance of eggplant germplasm resources, researching the waterlogging tolerance mechanism and breeding the waterlogging tolerance by researching the waterlogging morphological symptoms of the eggplant aiming at the current situation of weak foundation of the waterlogging tolerance research. The invention establishes the eggplant waterlogging tolerance evaluation system through visual morphological differences, can quickly screen out waterlogging tolerance germplasm resources from morphology, has simple and easy operation, objectively reflects the true condition of eggplant waterlogging, is a set of systematic method for researching the true condition of eggplant waterlogging at present, screens out waterlogging tolerance germplasm resources with strong waterlogging tolerance, is a batch of precious materials, and can be used for eggplant waterlogging tolerance breeding.
The invention is illustrated by the following specific examples:
1. the name and source of the test material are shown in Table 1
TABLE 1 materials name and Source
2. Test method
(1) Waterlogging tolerant phenotype grading and scoring standard
And (3) performing flooding treatment on the four-leaf and one-heart eggplant seedlings by adopting a plug flooding method, and flooding 2cm above the soil surface for 14 days. And (3) performing waterlogging recovery treatment after 14 days of flooding, performing phenotype observation on eggplant seedlings after 7 days of recovery, and selecting 2 evaluation indexes of leaf color, abscission and stem morphology for evaluation. And (3) carrying out quantitative grading on the 2 flat indexes, and formulating a grade score standard:
1) grading and scoring standard for color and shedding degree of leaves of waterlogged eggplant seedlings
A level: the blade basically recovers growth, or only the blade tip has weak withered and yellow phenomena. (score 0)
B stage: no withered leaves, and yellow leaves are less than or equal to 2. (score 1)
C level: the seedlings have recovered to normal growth, and no more than 3 withered leaves. (score 2 points)
D stage: most leaves of seedlings almost die, and only heart leaves are yellow-green. (score 3 points)
E, grade: all the leaves of the seedlings die, and no green leaves exist. (score 4 points)
2) Eggplant seedling stem morphology grade division and scoring standard
A level: the stalks grow normally at the flooded position without any abnormal growth phenomenon. (score 0)
B stage: the stem at the flooded position has the phenomenon of expansion and growth. (score 1)
C level: the stalks at the flooded position have the phenomenon of constriction and have the tendency of tissue necrosis. (score 2 points)
D stage: the stem is necrotic at the flooded place, and the plant is lodged. (score 3 points)
(2) Comprehensive membership function value of waterlogging tolerance phenotype
According to the grading and scoring standards of the color and the abscission of the leaves and the shape of the stems, 2 evaluation index scores of 16 eggplant seedlings are calculated for each material, and the average score of 2 evaluation indexes for each material is calculated, wherein the formula is as follows:
in the formula, XiExpressing the number of plants in each waterlogging grade; n is a radical ofiExpressing the scores corresponding to the waterlogging grades
Calculating various phenotype flood tolerance membership function values according to the average scores of the 2 evaluation indexes, accumulating the average number of the 2 kinds of flood tolerance membership function values of each material to obtain the average membership function value of the material, comparing the flood tolerance among different eggplant materials by using the average membership function value, and if the comprehensive membership function value is higher, indicating that the eggplant has stronger flood tolerance; the waterlogging tolerance membership function value XijThe fuzzy mathematics membership function value method is used, and the calculation formula is as follows:
the formula:
cold resistance membership function value, X, of j-th index of i-th typeijNumerical value, X, of the j-th index of the i-th typejmaxDenotes the maximum value of the j index, XjminRepresents the minimum value of the j index. If a certain index is inversely related to the flood tolerance, the flood tolerance membership degree can be calculated by using an inverse membership function.
The formula:
in the result, if 0. ltoreq.XijLess than or equal to 0.35, the material is a material with stronger waterlogging tolerance; if 0.35 < XijThe waterlogging tolerance is equal to or less than 0.65, and the material is a material with moderate waterlogging tolerance; if 0.65 < XijLess than or equal to 1.0, the material is a material with weaker waterlogging resistance.
3. Test results
Eggplant germplasm resource waterlogging tolerance identification result
(1) Identification result of leaf color and shedding waterlogging tolerance after waterlogging recovery treatment
TABLE 2 identification of leaf color and exfoliation waterlogging tolerance after waterlogging recovery treatment
(2) TABLE 3 evaluation of the morphological resistance to waterlogging of the stalks after the waterlogging recovery treatment
TABLE 3 evaluation of the morphological resistance to waterlogging of the stalks after the waterlogging recovery treatment
(3) TABLE 4 average membership function values and waterlogging tolerance after waterlogging recovery treatment
The method establishes the eggplant waterlogging tolerance evaluation system through visual morphological differences, can rapidly screen out waterlogging tolerance germplasm resources from morphology, is simple and easy to operate, objectively reflects the true condition of eggplant waterlogging, and is a set of systematic research method aiming at the true condition of eggplant waterlogging. The identification result table 4 shows that 81 parts of materials with strong waterlogging tolerance, 113 parts of materials with moderate waterlogging tolerance and 37 parts of materials with extremely poor waterlogging tolerance are screened by using the waterlogging tolerance evaluation system, are precious eggplant resources and can be used for waterlogging tolerance breeding of eggplants.
The above description of the present invention is intended to be illustrative. Various modifications, additions and substitutions for the specific embodiments described may be made by those skilled in the art without departing from the scope of the invention as defined in the accompanying claims.
Claims (6)
1. A method for rapidly identifying waterlogging tolerance of eggplants is characterized by comprising the following steps:
the method comprises the following steps: performing flooding treatment on the four-leaf and one-heart eggplant seedlings, and performing flooding for 14 days after 2cm of soil surface is flooded;
step two: carrying out waterlogging recovery treatment after flooding for 14 days, carrying out eggplant seedling phenotype observation after recovering for 7 days, and selecting 2 evaluation indexes of leaf color, abscission property and stem form for evaluation;
step three: quantitative grading is carried out by adopting 2 evaluation indexes of leaf color, abscission property and stem shape, and a grade scoring standard is formulated;
step four: evaluating the grade score of the eggplant seedlings in each material by using the grade score standard, and calculating the average score of each material;
step five: and calculating the waterlogging tolerance membership function value by using the calculated average score of each material, accumulating and calculating the average number of the 2 waterlogging tolerance membership function values of each material to obtain the average membership function value of the material, comparing the waterlogging tolerance of different eggplant materials by using the average membership function value, and if the comprehensive membership function value is higher, indicating that the waterlogging tolerance of the eggplant is stronger.
2. The method for rapidly identifying waterlogging tolerance of eggplant as claimed in claim 1, wherein: the first step is that 30 seeds are sown in each material in a hole tray, thinning and transplanting are carried out when eggplant seedlings grow to two leaves and one heart, 16 seedlings with the same growth vigor are selected for each material, flooding is carried out when the eggplant seedlings grow to four leaves and one heart, the hole tray is placed in a cement flooding pool to carry out flooding treatment, water is placed in the pool to 2cm above the surface of soil, the water level is kept constant during flooding, and the flooding treatment is carried out for 14 d.
3. The method for rapidly identifying waterlogging tolerance of eggplant as claimed in claim 1, wherein: and the second step is specifically that after the waterlogging treatment is carried out for 14 days, all water in the cement flooded waterlogging pond is drained, normal growth management is carried out on the eggplant seedlings, and after 7 days, grade score statistics is carried out on 2 evaluation indexes of leaf color and abscission performance of the eggplant seedlings and stem shape.
4. The method for rapidly identifying waterlogging tolerance of eggplant as claimed in claim 1, wherein: the waterlogging-resistant form evaluation indexes and the score standards of the eggplant seedlings in the third step are that the 2 evaluation indexes of leaf color, abscission property and stem form are adopted for quantitative grading, and the grade score standards are formulated:
(1) leaf color and exfoliation:
a level: the blade basically recovers growth, or only the blade tip has withered and yellow phenomena, and the score is 0;
b stage: no withered leaves, yellow leaves are less than or equal to 2 leaves, and the score is 1;
c level: the seedlings return to normal growth, withered leaves do not exceed 3 leaves, and the score is 2;
d stage: most leaves of seedlings almost die, only heart leaves are yellow green, and the score is 3;
e, grade: all the leaves of the seedlings die, no green leaves exist, and the score is 4;
(2) the shape of the stem at the flooded position:
a level: the stalks grow normally at the flooded position without any abnormal growth phenomenon, and the score is 0;
b stage: the stem at the flooded position has the phenomenon of expansion and growth, and the score is 1;
c level: the stem at the flooded position has a shrinkage phenomenon with a tissue necrosis trend, and the score is 2;
d stage: the stalks at the flooded place are necrotic, the plants are lodging, and the score is 3.
5. The method for rapidly identifying waterlogging tolerance of eggplant as claimed in claim 1, wherein: the average score calculation formula of each material in the fourth step is as follows:
in the formula, XiExpressing the number of plants in each waterlogging grade; n is a radical ofiAnd expressing the scores corresponding to the waterlogging grades.
6. The method for rapidly identifying waterlogging tolerance of eggplant as claimed in claim 1, wherein: in the fifth step, the waterlogging tolerance membership function value XijThe fuzzy mathematics membership function value method is used, and the calculation formula is as follows:
<mrow> <mover> <msub> <mi>X</mi> <mrow> <mi>i</mi> <mi>j</mi> </mrow> </msub> <mo>^</mo> </mover> <mo>=</mo> <mfrac> <mrow> <msub> <mi>X</mi> <mrow> <mi>i</mi> <mi>j</mi> </mrow> </msub> <mo>-</mo> <msub> <mi>X</mi> <mrow> <mi>j</mi> <mi>m</mi> <mi>i</mi> <mi>n</mi> </mrow> </msub> </mrow> <mrow> <msub> <mi>X</mi> <mrow> <mi>j</mi> <mi>m</mi> <mi>a</mi> <mi>x</mi> </mrow> </msub> <mo>-</mo> <msub> <mi>X</mi> <mrow> <mi>j</mi> <mi>m</mi> <mi>i</mi> <mi>n</mi> </mrow> </msub> </mrow> </mfrac> </mrow>
wherein,cold resistance membership function value, X, of j-th index of i-th typeijNumerical value, X, of the j-th index of the i-th typejmaxDenotes the maximum value of the j index, XjminA minimum value representing the j index;
if a certain index is negatively related to waterlogging tolerance, the waterlogging tolerance membership degree can be calculated by using an inverse membership function, and a formula is calculated:
<mrow> <mover> <msub> <mi>X</mi> <mrow> <mi>i</mi> <mi>j</mi> </mrow> </msub> <mo>^</mo> </mover> <mo>=</mo> <mn>1</mn> <mo>-</mo> <mfrac> <mrow> <msub> <mi>X</mi> <mrow> <mi>i</mi> <mi>j</mi> </mrow> </msub> <mo>-</mo> <msub> <mi>X</mi> <mrow> <mi>j</mi> <mi>min</mi> </mrow> </msub> </mrow> <mrow> <msub> <mi>X</mi> <mrow> <mi>j</mi> <mi>max</mi> </mrow> </msub> <mo>-</mo> <msub> <mi>X</mi> <mrow> <mi>j</mi> <mi>min</mi> </mrow> </msub> </mrow> </mfrac> </mrow>
wherein, if X is more than or equal to 0ijLess than or equal to 0.35, the material is a material with weaker waterlogging resistance; if 0.35 < XijThe waterlogging tolerance is equal to or less than 0.65, and the material is a material with moderate waterlogging tolerance; if 0.65 < XijLess than or equal to 1.0, the material is a material with stronger waterlogging resistance.
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| CN201710356927.2A CN107155666A (en) | 2017-05-19 | 2017-05-19 | A kind of method of eggplant resistance to overhead flooding injury Rapid identification |
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| CN113340820A (en) * | 2021-06-11 | 2021-09-03 | 上海辰山植物园 | Identification method for evaluating waterlogging tolerance of magnolia stelleriana by utilizing photosynthetic property and leaf color |
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