CN107151637A - The one plant of effectively bacillus cereus H B 1 of preventing and treating oriental cherry root knot and its application - Google Patents
The one plant of effectively bacillus cereus H B 1 of preventing and treating oriental cherry root knot and its application Download PDFInfo
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- CN107151637A CN107151637A CN201710358472.8A CN201710358472A CN107151637A CN 107151637 A CN107151637 A CN 107151637A CN 201710358472 A CN201710358472 A CN 201710358472A CN 107151637 A CN107151637 A CN 107151637A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
- C12R2001/085—Bacillus cereus
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/10—Animals; Substances produced thereby or obtained therefrom
Abstract
The bacterium bacterial strain of oriental cherry root knot is effectively prevented and treated the invention discloses one plant, Classification And Nomenclature is bacillus cereus (Bacillus cereus) H B 1, has been preserved in China typical culture collection center CCTCC, deposit number CCTCC NO：M 2017214, preservation day is on April 27th, 2017, preservation address：Wuhan, China Wuhan University.The Bacillus cereus strain H B 1 that the present invention is provided, are plant rhizosphere biological and ecological methods to prevent plant disease, pests, and erosion growth-promoting bacterium, have preferable prevention effect to oriental cherry root knot, while the growth to oriental cherry has growth-promoting functions.The condition of culture of Bacillus cereus strain H B 1 is simple, easily preserve, it is easy to industrialized production, with good development prospect.
Description
Technical field
The invention belongs to microbial technology field, and in particular to the bacillus cereus of one plant of effective preventing and treating oriental cherry root knot
(Bacillus cereus) H-B-1 and its application.
Background technology
Oriental cherry is world-renowned ornamencal flower and tree, is under the jurisdiction of the rose family (Rosaceae) cherry category (Cerasus), is distributed in north
The warm area of hemisphere, is north temperate zone plant.Oriental cherry system shallow root plant, likes slightly sour loose fertile, well-drained soil and the back of the body
The environment of wind direction sun moistening.China possesses the oriental cherry resource of very abundant, and considerably beyond Japan and neighboring countries, distribution is non-
Often extensively, all it is distributed from northeast to southwest, the high species of many of which ornamental value.In recent years oriental cherry because its plant type it is excellent
U.S., gorgeous pattern, florescence duration length and with high ornamental value the person that causes cultivating seedlings extensive attention, but with
Being continuously increased for cultivated area, the area of suffered disease infestation also constantly expands, wherein with oriental cherry root knot, oriental cherry shothole disease
And oriental cherry bleeding disease is the most serious.
Root knot is also referred to as root knot, root head cancer, clubroot, and it is as caused by Agrobacterium tumefaciems and with serious harm
Bacterial disease, the disease has different degrees of generation in worldwide.Song Yu pairs et al. research discoveries, soil Agrobacterium
Host range widely, 93 sections 331 category more than 643 can be infected and plant different plants, wherein to endanger dicotyledon
Based on, gymnosperm and monocotyledon can be also infected under specific condition.The infection way of root knot mainly passes through wound.
Site of pathological change is mainly root neck, lateral root and limb, and classical symptom is the tumour differed in position of disease formation shape size.Morbidity
The symptom at position initial stage is to be initially formed milky, knob small such as beans, afterwards with nursery stock growth knob by
Gradually grow up, color is gradually deep, surface becomes coarse by smooth, and then the quality become by internal lignifying is hard, shows dark brown
Color, irregular shape is become after withered cracking, and knurl body can surround root neck or branch when the disease occurs serious.It is ill early
The aerial part non-evident sympton of phase, nursery stock or tree body.Big with the change of tumour, plant imaginary root is tailed off, and tree vigo(u)r is entered by dying down by force
And yellowing leaf is caused, untimely defoliation.When falling ill serious, plant can be affected in terms of nutrient absorption and Water Transportation,
Plant strain growth slows down, and yield declines year by year, last until dead.
Oriental cherry root knot is caused by Agrobacterium tumefaciens, and very big loss is caused in production, is put within 1996 years
Domestic forest quarantine object list.It is that spherical or hemispherical knob is produced at rhizome that oriental cherry, which obtains most obvious symptom after being ill,
Knob is slowly grown up with the growth of nursery stock, has been had a strong impact on oriental cherry seedling growth speed and tree vigo(u)r, has been reduced ornamental article
Matter and commodity value.Disease causes when occurring light falls leaves in advance, can cause whole tree withered when serious, be referred to as oriental cherry by flower-grower
" cancer ".
It is main based on chemical prevention and biological control currently for the means of prevention of oriental cherry root knot.Utkhede RS etc.
(1993) root of morbidity treelet is handled with Kocide SD solution in apple field test, test group nodule number is as a result found
Reduced than control group；Wang Huimin etc. (1995) is by determining the suppression of bactericide and antibiotic to 3 kinds of bion Agrobacterium tumefaciems
Bacterium acts on, and research finds that streptomycin sulphate is all fine to the inhibitory action of whole crown gall bacterium；Thiram is to biological III type crown gall agriculture
All bacterial strains of bacillus have compared with high inhibition effect；Benzylpenicillin sodium salt only has good inhibiting effect to biological III type；Other medicaments are equal
Without effect.But long-term use chemical pesticide can bring a series of serious problems to environment and the ecological balance, and biological control
Favor significantly was enjoyed because its cost is low, pollute small, prevention effect in the last few years, some scientific workers prevent and treat work in root knot
Also variety classes and the significant biocontrol bacterial strain of prevention effect have been filtered out during work.Kerr is taught from the ill plant of peach root knot
Isolated bacterial strain K84 in rhizosphere soil, can effectively suppress the growth of pathological form Agrobacterium tumefaciens；Akira (2007) from
Isolated grape agrobacterium VAR03-1, no pathogenicity on the grape nursery stock of Japan, and a kind of bacteriocin can be produced, can
To suppress being formed as the tumour caused by pathogenic grape agrobacterium G-Ag-27 on tomato and grape.
Bacillus because cultivation cycle it is short, it is convenient for production be increasingly becoming the dominant strains of biocontrol microorganisms.It is class production bud
The gram-positive bacterium of spore, aerobic or amphimicrobian life can produce heat-resisting, drought-enduring, uvioresistant and anti-organic molten
The endospore of agent, produces gemma and can be made into the various formulations such as pulvis, wettable powder, and it is mixed with chemical pesticide after do not lose
It is living, it is preferable screening of biocontrol agents object.
Clover is the model plant for being generally used for hospital's Bacteria infecting respiratory Pathogenicity.Laura uses one within 2002
Plant and clover and mouse are inoculated with respectively in the CF pathogen Psudomnoas aeruginosa often detected in patient body, compare
Result of study finds that clover can replace mouse as model plant and determine toxicity of the bacterium to mammal, or even clover mould
Type shows the sensitivity higher than mouse models (Laura et al., 2002).Then, Steve has been done equally to Bcc
Compare test, it is found that the incidences of disease of the Bcc on clover is proportionate with the result on mouse, measure can be used as instead of mouse
Bcc is to the model plant of animal toxicity (Steve et al., 2003).With clover model can simple and fast detect Bcc bacterium
It is pathogenic whether strain has to mammal, and this has established solid foundation for our the safe utilization Bc in agricultural and environmental protection.
In the last few years, the research about bacillus in oriental cherry root knot field of biological control was rarely reported.Therefore, by bud
The biological control that spore bacillus is used for oriental cherry root knot works, and is conducive to preferably preventing and treating the disease.
The content of the invention
Goal of the invention：For the deficiencies in the prior art, it is an object of the invention to provide a kind of effectively preventing and treating oriental cherry
The bacillus cereus H-B-1 of root knot.It is a further object of the present invention to provide the purposes of above-mentioned bacillus cereus.
Technical scheme：In order to solve the above technical problems, the technical solution adopted by the present invention is as follows：
The bacterium bacterial strain of one plant of effective preventing and treating oriental cherry root knot, Classification And Nomenclature is bacillus cereus (Bacillus
Cereus) H-B-1, has been preserved in China typical culture collection center CCTCC, deposit number CCTCC NO：M 2017214,
Preservation day is on April 27th, 2017, preservation address：Wuhan, China Wuhan University.
Bacillus cereus (Bacillus cereus) H-B-1 of effective preventing and treating oriental cherry root knot is one plant from suffering from root
The biocontrol bacteria bacterial strain to crown gall germ with antagonism that the screening of carninomatosis peach rhizosphere soil is obtained.Bacillus cereus
(Bacillus cereus) H-B-1 bacterium colonies on beef-protein medium flat board are smaller, faint yellow, the general 2- of diameter
3mm, circular, edge is irregular, the smooth moistening in surface, thalline direct rod shape, and size is 0.7 × (1~2) μm.Gram's staining table
It is now feminine gender；The most of sequence 1444bp of 16SrDNA are measured, specifically as shown in SEQ ID NO.1.By surveyed 16SrDNA sequences
Compared by BLAST, homology very high close strain sequence can be found in GenBank.With H-B-1 bacterial strain similitudes
Highest is Bacillus thuringiensis and Bacillus cereus, and homology all reaches 99%.Build chadogram hair
Existing, H-B-1 and Bacillus cereus belong to a hereditary branch together, and affiliation is sufficiently close to.Pass through physiological and biochemical test
It was found that the bacterium indole reaction is feminine gender；Catalase reaction result is the positive；Clark and Lubsreaction result is the positive；Starch Hydrolysis is tested
For the positive；V-P experiments, nitrate reduction reaction and citrate reaction result also all present positive；Parasporal crystal dyeing is found
The bacterium does not have parasporal crystal；Therefore, it is determined that the bacterium is bacillus cereus (Bacillus cereus), waxy gemma is named as
Bacillus (Bacillus cereus) H-B-1.
Above-mentioned Bacillus cereus strain H-B-1 and its metabolite are to oriental cherry root knot pathogen Agrobacterium tumefaciems
(Agrobacterium tumefaciens) has certain bacteriostasis, therefore, it is possible to efficiently preventing and treating oriental cherry root knot.
Above-mentioned Bacillus cereus strain H-B-1 also has somatotrophic effect to oriental cherry.
The above-mentioned preferable fermentation condition of Bacillus cereus strain H-B-1 prevention effects is as follows：
Culture medium：Per 1000mL distilled water, 3g containing beef extract, peptone 10g, sodium chloride 5g, pH7.2.
Process conditions are：PH6~7, throughput 1：1,28 DEG C~30 DEG C, 180~200 revs/min, 24 hours of shaking speed.
The zymotic fluid obtained by above-mentioned zymotechnique is a kind of microbial bactericide.The zymotic fluid is with water according to 1:5
Dilution proportion simultaneously adds appropriate soil and breaks into mud naked of plant is carried out dipping in slurry processing and can play its bactericidal action.
Beneficial effect：Compared with prior art, the Bacillus cereus strain H-B-1 that the present invention is provided, is plant rhizosphere
Biological and ecological methods to prevent plant disease, pests, and erosion growth-promoting bacterium, has preferable prevention effect to oriental cherry root knot, while the growth to oriental cherry has growth-promoting functions.This is waxy
Bacillus strain H-B-1 condition of culture is simple, easily preserve, it is easy to industrialized production, with good development prospect.
Brief description of the drawings
Fig. 1 is Bacillus cereus strain H-B-1 antibacterial result figure；
Fig. 2 is Bacillus cereus strain H-B-1 antagonism result figure of the ferment filtrate to Agrobacterium tumefaciens；
Fig. 3 is resistant effect result figures of the Bacillus cereus strain H-B-1 to greenhouse indicator plant root knot.
Embodiment
With reference to specific embodiment, the present invention is described further., can be more preferably geographical according to following embodiments
The solution present invention.However, as it will be easily appreciated by one skilled in the art that specific material proportion, process conditions described by embodiment
And its result is merely to illustrate the present invention, without should be also without limitation on the present invention described in detail in claims.
Embodiment 1：Bacillus cereus strain H-B-1 determines them by agar diffusion method and root knot bacteria strain is grown
Inhibitory action.
The bacterium that the agrobacterium tumefaciens for purifying culture are separated with soil is made 107~108Cfu/mL bacteria suspensions, will
Tumorigenesis Agrobacterium A-4 bacteria suspensions are mixed by 5% inoculum concentration with 523 culture mediums of improvement, are shaken up.25mL is added per ware and is mixed with tumorigenesis
The culture medium of improvement 523 of Agrobacterium, 2~3 holes are made a call to after solidification on culture medium with card punch, add 130 μ L's in every hole
Soil bacteria bacteria suspension, is placed in 28 DEG C of culture 24h~48h, surveys its antibacterial circle diameter.As a result show as shown in figure 1, left figure is suppression
Bacterium is schemed, and right figure compares for CK, it is seen that Bacillus cereus strain H-B-1 good antimicrobial effect, bacteriostatic diameter is 7.67cm, and it presses down
Bacterium activity is substantially better than other bacterial strain bacteriostasis.
Embodiment 2：Bacillus cereus strain H-B-1 zymotic fluids carry out the survey of flat board bacteriostasis with root knot bacteria strain
It is fixed.
The glycerol stocks liquid of Bacillus cereus strain H-B-1 bacterial strains is inoculated into beef extract albumen by 2% inoculum concentration
In peptone fluid nutrient medium, training 24h is shaken.Ferment product is produced into bacterial fermentation filtrate with 0.22 μm of biofilter filtering.
The bacterial fermentation filtrate of every plant of bacterium and culture medium (523 culture 25mL of improvement) are mixed by 20% inoculum concentration, diameter is taken
0.6cm filter paper picks the bacteria suspension of Agrobacterium tumefaciens, and appropriateness is placed in after air-drying and is mixed with the flat board of bacterial fermentation filtrate,
Per 2~3 filter papers of plate, colony diameter is measured after cultivating 48h in incubator.As a result find that bacterial strain H-B-1 shows one
Fixed antagonistic activity.The ferment filtrate of bacterial strain is to the bacteriostatic activity of root knot bacteria strain with the pathogen bacterium measured by 24h~48h
Fall diameter for 1.05cm, less than the 2.37cm of control space management (Fig. 2, left figure is result figure, and right figure compares for CK).
Embodiment 3：Resistant effects of the Bacillus cereus strain H-B-1 to greenhouse indicator plant root knot
Tomato seedling is bought, is placed in mixed cultivating soil and cultivates.Inoculation (choosing when seedling stretches out soil face 15cm or so
Take the healthy and strong seedling that growing way is similar), half an hour takes out bacterium (6 plants of soil bacterias of separation) waiting before inoculation, makes culture bacterium
Temperature is recovered to room temperature, then starts inoculation.First by dissecting needle on alcolhol burner incineration, treat that dissecting needle is cooled to room temperature
Afterwards, the bacterium after picking is isolated and purified is pierced to the stem of seedling, and sterilized water is dipped with absorbent cotton wool and is tied up at inoculation with sealed membrane
Tie up, 5~7d of moisturizing；It is inoculated with oriental cherry crown gall germ first half hour and takes out bacterium waiting, the temperature of culture bacterium is recovered to room temperature, then
Start inoculation.First by dissecting needle on alcolhol burner incineration, after dissecting needle is cooled to room temperature, picking isolate and purify after it is thin
At bacteria thorn to seedling rhizome, sterilized water is dipped with absorbent cotton wool sealed membrane bondage, 5~7d of moisturizing are used at inoculation.Inoculating strain
H-B-1 and cause of disease bacteria strain；Individually inoculation cause of disease bacteria strain is as compareing, not connecing bacterium as blank control, and totally three are handled,
5 repetitions are done in each processing, and growth is cultivated in room temperature is the greenhouse of 5 DEG C of 23 scholar.Morbidity is seen whether after 3 weeks.As a result find
The obvious enlargement of base of the plant of peach root knot germ is only inoculated with, while the base of the plant of inoculating strain H-B-1 and cause of disease bacteria strain
Slightly enlargement, show H-B-1 have certain resistant effect (Fig. 3, left to be only inoculated with pathogen, right is is inoculated with H-B-1 simultaneously
And pathogen).
Embodiment 4：Bacillus cereus strain H-B-1 fields prevent and treat the disease resisting effect of oriental cherry root knot
Bacillus cereus strain H-B-1 is applied to the field field plots that root knot seriously occurs, female bacterium solution 2L is taken,
Add water in 10L barrels of constant volume, dilute 5 times, stir and evenly mix, plus appropriate soil stirring makes slurries in thick.By naked oriental cherry seedling
It is put into one by one in bucket and submerges root, taken out after one layer of mud is wrapped up on surface, is put into the soil pit plant dug in advance.Result of the test
Show that the bacterial strain prevents and treats root knot effect to the healthy oriental cherry of annual new afforestation with good.Slurry processes are stained with using the bacterium in root
100 oriental cherry nursery stocks of strain, its incidence of disease is 13.3%, far below the 53.3% of space management.Root nodule diameter is averaged
18.8mm, less than the 28.58mm (table 1) of space management.Therefore, it is determined that the bacterial strain can effectively prevent and treat oriental cherry root knot.Except this
Outside H-B-1 bacterial strains to annual oriental cherry seedling also have excellent growth-promoting functions (table 2).
Table 1, annual oriental cherry experiment seedling morbidity statistics
| Bacterial strain | The incidence of disease (%) | Root nodule diameter (mm) | Survival rate (%) |
| CK | 53.3 | 28.58 | 51 |
| H-B-1 | 13.3 | 18.8 | 91 |
Table 2, annual oriental cherry seedling increment statistics
| Bacterial strain | Height of seedling (cm) | Ground diameter (mm) |
| CK | 4.1±2.3b | 4.4±2.1b |
| H-B-1 | 37.2±19.2a | 7.71±2.7a |
Embodiment 5：Bacillus cereus strain H-B-1 is to onion Pathogenicity.
Pathogenicity takes Bacillus cereus strain H-B-1 bacterium with syringe with reference to the method for (2005) such as Sijam
Suspension (1 × 107~108Cfu/mL) two point symmetries injection (depth is 1~2cm) on onion bulb stem, every injection volume is 1mL.
Control is processed as with aqua sterilisa.All processing are repeated 3 times, and are placed in 26 DEG C of cultures, inspection result after 4~5 days.Onion bulb stem connects
Plant experiment to show, injection inoculation Bacillus cereus strain H-B-1 does not cause rotting for onion bulb stem, simply at inoculation slightly
There is shrivelled depression.Bacillus cereus strain H-B-1 described above is not plant pathogenetic bacteria, has security to plant.
Embodiment 6：Inoculation tests of the Bacillus cereus strain H-B-1 to clover.
H-B-1 is inoculated into NB culture mediums, (150mrp) culture 24h is shaken at 30 DEG C, bacteria suspension is adjusted with sterilized water
Concentration is to 109Left and right is used to be inoculated with.Prepare the alfalfa seed to be inoculated with (Laura et al., 2002) with Laura method.
Alfalfa seed is soaked into 20 minutes (about 10mL soaks 300 seeds) with 98% concentrated sulfuric acid, 4 are cleaned with 500mL aqua sterilisas
It is secondary, then steep in 60mL aqua sterilisas, (150prm) is shaken at 32 DEG C and is soaked seed 6~8 hours, then clean 2 times, change same after water
CMC model is stayed overnight.Next day cleans after 2 times (each 60mL water) with aqua sterilisa again, and the water agar to 1% is put with aseptic nipper
Flat board is first-class to be seeded, and 20 seeds are put per ware.Alfalfa seed on water agar plate is pierced one with transfer needle on cotyledon
Individual aperture, takes the ready bacteria suspension points of 20mL in wound, per inoculation 3 repetitions, often repeatedly 20 seeds, if 2 kinds
CK processing, wherein 1 is not made any processing, another l stab inoculation sterilized water.Seed after inoculation 37 DEG C, it is 70% relatively wet
Cultivated in degree, the growth cabinet of timing illumination (12h illumination, 12h is dark) condition, the incidence of disease is checked after 7 days.
Clover inoculation result shows that either CK or inoculation is handled, without disease symptom (vaccination necrosis nearby, Huang
Change and root be abnormal) occur.Clover be that instead of mouse as determine Bcc to the model plant of animal toxicity (Steve et al.,
2003), with clover model can simple and fast detect Bcc bacterial strains to mammal whether have it is pathogenic.Above-mentioned experiment knot
Fruit explanation Bacillus cereus strain H-B-1 does not have toxic to mammal.
SEQUENCE LISTING
<110>Nanjing Forestry University
<120>The one plant of effectively bacillus cereus H-B-1 of preventing and treating oriental cherry root knot and its application
<130> 100
<160> 1
<170> PatentIn version 3.3
<210> 1
<211> 1444
<212> DNA
<213> Bacillus cereus
<400> 1
gcggcgtgcc tatacatgca agtcgagcga atggattaag agcttgctct tatgaagtta 60
gcggcggacg ggtgagtaac acgtgggtaa cctgcccata agactgggat aactccggga 120
aaccggggct aataccggat aacattttga accgcatggt tcgaaattga aaggcggctt 180
cggctgtcac ttatggatgg acccgcgtcg cattagctag ttggtgaggt aacggctcac 240
caaggcaacg atgcgtagcc gacctgagag ggtgatcggc cacactggga ctgagacacg 300
gcccagactc ctacgggagg cagcagtagg gaatcttccg caatggacga aagtctgacg 360
gagcaacgcc gcgtgagtga tgaaggcttt cgggtcgtaa aactctgttg ttagggaaga 420
acaagtgcta gttgaataag ctggcacctt gacggtacct aaccagaaag ccacggctaa 480
ctacgtgcca gcagccgcgg taatacgtag gtggcaagcg ttatccggaa ttattgggcg 540
taaagcgcgc gcaggtggtt tcttaagtct gatgtgaaag cccacggctc aaccgtggag 600
ggtcattgga aactgggaga cttgagtgca gaagaggaaa gtggaattcc atgtgtagcg 660
gtgaaatgcg tagagatatg gaggaacacc agtggcgaag gcgactttct ggtctgtaac 720
tgacactgag gcgcgaaagc gtggggagca aacaggatta gataccctgg tagtccacgc 780
cgtaaacgat gagtgctaag tgttagaggg tttccgccct ttagtgctga agttaacgca 840
ttaagcactc cgcctgggga gtacggccgc aaggctgaaa ctcaaaggaa ttgacggggg 900
cccgcacaag cggtggagca tgtggtttaa ttcgaagcaa cgcgaagaac cttaccaggt 960
cttgacatcc tctgaaaacc ctagagatag ggcttctcct tcgggagcag agtgacaggt 1020
ggtgcatggt tgtcgtcagc tcgtgtcgtg agatgttggg ttaagtcccg caacgagcgc 1080
aacccttgat cttagttgcc atcattaagt tgggcactct aaggtgactg ccggtgacaa 1140
accggaggaa ggtggggatg acgtcaaatc atcatgcccc ttatgacctg ggctacacac 1200
gtgctacaat ggacggtaca aagagctgca agaccgcgag gtggagctaa tctcataaaa 1260
ccgttctcag ttcggattgt aggctgcaac tcgcctacat gaagctggaa tcgctagtaa 1320
tcgcggatca gcatgccgcg gtgaatacgt tcccgggcct tgtacacacc gcccgtcaca 1380
ccacgagagt ttgtaacacc cgaagtcggt ggggtaacct ttttggagcc agccgcctaa 1440
ggtg 1444
Claims (10)
1. one plant of bacterium bacterial strain for effectively preventing and treating oriental cherry root knot, Classification And Nomenclature is bacillus cereus (Bacillus
Cereus) H-B-1, has been preserved in China typical culture collection center CCTCC, deposit number CCTCC NO：M 2017214,
Preservation day is on April 27th, 2017, preservation address：Wuhan, China Wuhan University.
2. applications of the Bacillus cereus strain H-B-1 in preventing and treating oriental cherry root knot described in claim 1.
3. application of the metabolite of the Bacillus cereus strain H-B-1 described in claim 1 in preventing and treating oriental cherry root knot.
4. application according to claim 3, the zymotic fluid that described metabolite is Bacillus cereus strain H-B-1.
5. applications of the Bacillus cereus strain H-B-1 in Agrobacterium tumefaciems is suppressed described in claim 1.
6. application of the metabolite of the Bacillus cereus strain H-B-1 described in claim 1 in Agrobacterium tumefaciems is suppressed.
7. application according to claim 6, the zymotic fluid that described metabolite is Bacillus cereus strain H-B-1.
8. applications of the Bacillus cereus strain H-B-1 in oriental cherry growth is promoted described in claim 1.
9. application of the metabolite of the Bacillus cereus strain H-B-1 described in claim 1 in oriental cherry growth is promoted.
10. application according to claim 9, the zymotic fluid that described metabolite is Bacillus cereus strain H-B-1.
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| CN112746035A (en) * | 2020-11-30 | 2021-05-04 | 宁波城市职业技术学院 | Paenibacillus with bacteriostatic activity and extraction method and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105420167A (en) * | 2015-12-30 | 2016-03-23 | 苏州大学 | Bacillus cereus and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112746035A (en) * | 2020-11-30 | 2021-05-04 | 宁波城市职业技术学院 | Paenibacillus with bacteriostatic activity and extraction method and application thereof |
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