CN107144488B - A kind of fish gastrointestinal content quick quantitative analytic method and its application - Google Patents
A kind of fish gastrointestinal content quick quantitative analytic method and its application Download PDFInfo
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Abstract
The invention discloses a kind of fish gastrointestinal content quick quantitative analytic method and its applications in fish nutrition ecological study.Anatomical isolation fish gastrointestinal content of the present invention judges in stomach and intestine with the presence or absence of bulk food residuum or the difficult miniature food source distinguished；By weighing the weight in wet base of each component weight in wet base and the miniature food source of mixing in bulk food residuum respectively, main food source component each in content is divided with relative weight percents；The miniature food source component in mixing food group is divided with relative volume percentage；Food group weight percent is mixed in conjunction with actual measurement, the actual ratio of content total amount shared by each miniature food source is restored, keeps whole food source components in content unified in dimension.The results show that can be formed to different water body Mesichthyes feeding habits present method solves the influence that individual of sample size, feeding method and food source difference analyze feeding habits of fishes and carry out quantitative analysis, and convenient for the plasmodium cynomolgi between different each fingerlings.The method of the present invention has widely applicable, simple operation and other advantages, provides important technological means for ecology of fishes research.
Description
Technical field
The present invention relates to biological nutrition ecological study technical fields, more particularly, to a kind of fish gastrointestinal content
Quick quantitative analytic method and its application.
Background technique
The standard method of modern ecology of fishes Mesichthyes preferred plant species is gastrointestinal content analytic approach, utilizes the method one
Aspect can estimate the trophic level of the trophic structure and every kind of fish of Fish Communities in group, further study ecosystem
On the other hand substance circulation in system on food chain and food web is researching fish population consumption quantity of food, i.e. biology energy
Revenue and expenditure problem.
In existing research method, the analysis of fish gastrointestinal content can pass through the frequency of occurrences of certain class food source, number of individuals, body
Long-pending and mass percent determines.
The frequency of occurrences is the percentage for the total stomach number of stomach number Zhan (non-empty stomach number) for containing a certain food ingredients.If food kind
Class is easy determination, then this method is rapid, easy.But it is only capable of roughly reflecting a side of feeding habits of fishes, i.e., fish are to certain
The fancy grade of kind food, cannot clearly express the ratio that a certain group food ingredient accounts for stomach content quantity and volume.
Number of individuals composition is that the number of individuals of a certain group food ingredient in stomach content accounts for food composition total number in stomach inclusion
Percentage, the method be suitable for detection food source individual specification similar in fish, such as using planktonic organism as ichthyophagy class, detecting food
When the more miscellaneous fish of property, number of individuals method may exaggerate the importance of small-sized biological food source, and because small-sized biological digests rapidly,
Accurate counting is difficult in detection.Further, since food is digested analysis in enteron aisle, single food individual may be counted repeatedly
Number, if Annelida food source can fragment into multiple similar individuals, to expand the food importance of certain class bion.
Weight and volume percentage refers to that the corrigendum weight and volume of a certain group food accounts for gastrointestinal content total weight and total
The percentage of volume, the importance of gravimetric method easily excessively high single big individual food ingredients of estimation in the important Journal of Sex Research of food,
Miniature organism is difficult to weigh when analyzing inmature fish and small-sized ominivore-fish.
Volumetric method can be used classification measuring appliance and food ingredients be separated from content and are calculated volume, for being difficult to
The small-sized food individual of volume is directly calculated, then the total volume of computable number food individual calculates the average external volume of individual,
Although percent by volume method can more objectively react food composition, in shell-fish of the analysis containing contour structures complexity or there is heavily fortified point
When the mollusk food lumps of hard coat, volumetric method is complicated for operation and sizeable device for volume measurement is also difficult to make.
The analysis method of existing fish stomach content, and do not have reasonable versatility, it is not able to satisfy comprehensive detection water
In the ecosystem the problem of different fish food source compositions, therefore, it is badly in need of a kind of fish stomach and intestine widely applicable, easy to operate
Content quick quantitative analytic method.
Summary of the invention
The technical problem to be solved by the present invention is to the analysis methods for existing fish stomach content not to be able to satisfy unified point
The technological deficiency for analysing different waters fish foods source composition, provides a kind of fish gastrointestinal content quick quantitative analytic method.
Another technical problem to be solved by the present invention is that providing the method in ecology of fishes Mesichthyes preferred plant species side
The application in face.Using the method for the present invention, the feeding habits of different fingerlings can be formed and carry out quantitative analysis, and convenient between different fingerlings
Food source difference is compared, and this method can be used for the researchs such as food web building and ecosystem energy flow.
In order to achieve the above objectives, the present invention provides a kind of river fishes gastrointestinal content quick quantitative analytic method, including
Following steps:
S1. anatomical isolation fish gastrointestinal content carries out taxonomic identification to its each component, judges in stomach and intestine with the presence or absence of can
Debate bulk food residuum or the difficult miniature food source distinguished；
If there is no bulk food residuum can be debated, it is directly entered step S3 and S4；
If there is no the miniature food source that hardly possible is distinguished, the detection of step S2 is only carried out；
It is coexisted if the miniature food source that bulk food residuum and hardly possible are distinguished can be debated, the miniature food source that the remaining difficulty of collection is distinguished is as mixed
Food group is closed, the detection of step S2 to S4 is carried out；
S2. the weight in wet base for weighing each component in bulk food residuum claims the mixing food group of collection as one-component
Weight carries out Preliminary division to main food source component each in content with weight percent；
S3. it takes part mixing food group to be placed under optical microscopy and carries out taxonomic identification, calculate each miniature food source in tally
Volume compares the miniature food source component in mixing food group with volume basis and is divided；
S4. food group weight percent is mixed according to gained, restores the actual ratio of content total amount shared by each miniature food source,
Keep whole food source components unified in dimension, quantitative fish gastrointestinal content.
Preferably, step S1 is to split fish sample from abdomen to take out gastrointestinal tissue, gastrointestinal wall is cut off with dissecting scissors, with solution
Content in cut open cutter and tweezers scraping stomach and intestine, retains effectively (plumpness > 20%) content sample, is placed in glass dish, in body
The bulk food 1~i of residuum that can directly pick in content is separated under formula anatomical lens, collects the remaining miniature food by that can not recognize
The mixing food group j of source composition；
Step S1 and step S2 is to classify to reflect into body row to the bulk 1~i of food residuum picked out under body formula anatomical lens
It is fixed, and weighing W is carried out to each component1~Wi(weight in wet base g, similarly hereinafter), the mixing food group j that residue can not recognize claim as one-component
Weight WMixing, by calculating W1~WiAnd WMixingAccount for content gross weight WAlwaysPercentage, obtain the 1~i of food source component identified and do not know
Not Hun He the group of food j relatively heavy magnitude, be calculated as RG respectively1~RGiAnd RGMixing(%), calculation method is as follows:
WAlways(g)=W1+W2…+Wi+WMixing
Step S3 is that a part in mixing food group j is taken to be placed in 20mm × 20mm (long × wide) glass counting plate surface, glass
It counts plate face and is divided into 10 × 10 counting lattice (4mm2/ lattice), it is diluted and is stirred evenly with water (distilled water), press coverslip
Miniature food source is set to generate deformation, under dislocation optical microscopy, first full sheet counts and is greater than 50 μm of miniature food under 10 × 10 times of mirrors
Source, full sheet counts the miniature food source less than 50 μm under 10 × 40 times of mirrors, to the miniature food source in the mixing food group j observed
1~m carries out taxonomic identification；
Step S4 is (rectangle, triangle, trapezoidal, ellipse according to the approximate geometry of all kinds of miniature food sources under the microscope
Circle) estimate its surface area S (μm2), due to being pressed deformation by coverslip, each miniature food source height H (μm) can be considered unanimously, count
It can directly reduce when calculating volume, miniature food source j in gained mixing food group j1~jmApproximation surface product be Sj1~Sjm(μm2), body
Product is Vj1~Vjm(μm3), further calculate the percent by volume RV of content total amount shared by each miniature food source in mixing food groupj1
~RVjm(%):
Vim(μm3)=SxH
In the method for the present invention, the bulk food residuum includes: various fish, shell-fish necton, mollusk, water
Raw and terrestrial larva, coastal waters crinosity annelid, aquatic borne plant debris and/or sediment humus fragment.
Preferably, it in technical scheme steps S1 and step S2 of the present invention, can directly be picked under body formula anatomical lens
Bulk food residuum includes: various fish, the shell-fish necton such as shrimp, crab, molluscan muscle and broken shell, aquatic insect
Larva and littoral zone terrestrial insect, clam worm, aquatic borne plant debris, are sunk at extra large naiad and Sabellidae etc. coastal waters crinosity annelid
Product humus fragment；
Preferably, technical scheme steps S3 and step S4 of the present invention, taxonomic identification is miniature under an optical microscope
Food residuum includes: phytoplankton (based on cyanobacteria, Chlorophyta and planktonic diatom), zooplankter (branch pin class, Copepods, wheel
Worm, protozoan), attached algae (based on grow nonparasitically upon another plant diatom and silkweed), organic debris (degrading plant, fungi, microorganism
Film), suspended particulate substance, Sediments.
Preferably, technical solution of the present invention further relates to restore incomplete food source weight to quilt except step S1 to S4
The step of preying on preceding complete individuals weight and method, can preferably eliminate the error that each food source is generated by digestion process, the side
Method include the following: 1) the complete individuals average weight that with document report or identical waters is acquired, as food source such in content
The corrigendum weight of incomplete individual；2) certain class food source number of individuals more (such as Diptera, Ephemerida, Decapoda, Amphipoda in content
Deng), the average weight for retaining relatively complete individual can be screened as corrigendum weight；3) part of the food residuum (body length < 3mm, such as
Oligochaetes, chironomus larvas, microcrustacean etc.) it is difficult to weigh because individual is too small, its approximate geometry can be first estimated, in vacation
Determine specific gravity be 1 in the case where, its weight is estimated according to " body length-weight " relationship；4) part of the food residuum distinguish by form difficulty, can be right
Its remain (such as aquatic insect, shell-fish and Polychaeta) head capsule, instep limb, circumorbital bone, bristle, kiss, parapodum organ length into
Row measurement, according to " organ length-body length-volume/weight " empirical equation, estimates weight before the food source prey；5) it collects and includes
Heavy mollusk casing fragments in object, according to the weight ratio between mollusk shell and muscle, before restoring its prey
Weight.
The beneficial effects of the present invention are:
(1) it reduces tradition and includes object detecting method in size of food, volume, weight, frequency of occurrences statistic processes
Complexity simplifies taxonomic identification and quantitative analysis step.
(2) particularity of traditional single gastrointestinal content analysis method when analyzing different feeding fishes is overcome, it can
Fish carry out united analysis in the form of ingesting to difference, and testing result has extensive comparativity.
(3) use the method for eliminating individual weight error, will digest food residuum be restored to it is complete before prey
Individual weight keeps quantitative analysis results more accurate.
(4) present invention can rapidly analyze the gastrointestinal content of fish, be aquatic ecosystem Mesichthyes food web
Research provide convenient and fast food habits method.
Detailed description of the invention
Fig. 1 the method for the present invention step schematic diagram.
Component identification in source is shared the meal in the middle part of Fig. 2 snakehead gastrointestinal content.
Component identification in source is shared the meal in the middle part of Fig. 3 opsariichthys bidens gastrointestinal content.
Component identification in source is shared the meal in the middle part of Fig. 4 carp gastrointestinal content.
Component identification in source is shared the meal in the middle part of Fig. 5 flathead gastrointestinal content.
Specific embodiment
The method of the present invention is further illustrated in the following with reference to the drawings and specific embodiments.Following embodiment and attached drawing are only used for showing
Example property explanation, is not considered as limiting the invention.Unless stated otherwise, reagent raw material used in following embodiments is normal
The life reagent raw material that commercially available or commercial sources obtain is advised, unless stated otherwise, method and apparatus used in following embodiments is
Method and apparatus commonly used in the art.The method of the present invention step schematic diagram is as shown in Figure 1.
Food habits of the embodiment 1 to predacious fish snakehead
The snakehead sample that the present embodiment uses is in Guangdong Province's Xinfengjiang Reservoir random acquisition.
S1. taking-up stomach and intestine are splitted from abdomen, cuts off gastrointestinal wall with dissecting scissors, included in scalpel and tweezers scraping stomach and intestine
Object retains 8 effective (plumpness > 20%) content samples, is placed in glass dish, to big in sample under body formula anatomical lens
Block food residuum is separated.
S2. taxonomic identification is carried out to the i bulk food residuum picked out under body formula anatomical lens, according to the main of food source
Morphological feature and classification organ, the present embodiment identify 4 kinds of fish altogether: sub- mound kiss Acenthogobius flavimanus, wide fin Lap, striped two must Barb, mud
Loach；1 kind of shell-fish: Macrobrachium nipponensis；2 section of aquatic insect larva: Chun Ting section and Cordulegasteridae, food source composition are as shown in Figure 2.From food
Judging in integrality, the prey time of fish and shrimp is shorter, and individual is relatively complete, it can directly weigh, and Odonata larva limbs
It is broken, it has heavy head capsule (comprising mouthpart) only and instep limb is also more complete, it is completely a according to Chun Ting section and Cordulegasteridae larva
" instep limb length-weight " relationship of body, the individual weight before its prey is calculated with instep limb remaining in content.In addition, because of meat
Feeding fish is not ingested directly miniature food source, and under an optical microscope to a thick mixing food group microscopy for remaining gastric juice package
Any food sign is not found, therefore ignores mixing food group proportion.It is 0.0001g's in sensibility reciprocal as unit of individual of sample
Each food source weight in wet base W identified is weighed on electronic balance1~W6(g), its relative weight percents RG is calculated1~RG6。
Calculation method is as follows:
WAlways(g)=W1+W2…+Wi+WMixing
Snakehead gastrointestinal content quantitative analysis results are as shown in table 1, and Major Foods type is successively are as follows: fish 75.53%, shrimp
19.71%, dragonfly larva 4.75%；Successively according to the sequence of each food source component proportion are as follows: sub- mound kiss Acenthogobius flavimanus 31.91%,
Striped two must Barb 20.99%, Macrobrachium nipponensis 19.71%, wide fin Lap 14.87%, loach 7.76%, Chun Ting section 2.42%, big dragonfly
Section 2.33%.
The quantitative analysis results of each food source component in 1 snakehead gastrointestinal content of table
Food habits of the embodiment 2 to insect feeding fish opsariichthys bidens
The present embodiment opsariichthys bidens sample random acquisition is in Guangdong Province Liu Xihe, stomach and intestine dissection and content sampling process and behaviour
Make step, calculation method with embodiment 1.6 section of aquatic insect larva: hydropsychidae, multiple spurs phryganeid section, four section Fu is identified altogether
Section, flat Fu section, Chun Ting section, Corixidae；2 kinds of terrestrial insect: grasshopper, ant；1 kind of: Kuan Qi Lap (juvenile fish) of fish.With carnivorous fish
The fish of big individual, shrimp food source are different in class stomach, in insect feeding fish gastrointestinal content mostly based on aquatic insect larva, food source
Though individual is small, quantity is more, and in addition to the calculation method of Chun Ting section residuum is with embodiment 1, the weight of other small individual insects is calculated,
It takes and retains relatively complete insect individual, the complete individuals before prey is represented with the Weight averages of these individuals in content
Weight.In addition, insect feeding fish also grow nonparasitically upon another plant by less miniature food source of directly ingesting, the part that spectroscopy is found under optical microscopy
Algae and canebreak are taken in when being episite, therefore as predacious fish, ignore residual mixing food group institute's accounting in stomach and intestine
Example.Opsariichthys bidens food source forms as shown in figure 3, gastrointestinal content quantitative analysis results are as shown in table 2, and Major Foods type is successively
Are as follows: aquatic insect 73.60%, terrestrial insect 19.56%, fish 6.83%；Successively according to the sequence of each food source component proportion are as follows:
Trichoptera larva 31.47% (hydropsychidae 16.60%, multiple spurs phryganeid section 14.87%), (the four section Fu of Ephemerida larva 25.52%
Section 17.58%, flat Fu section 7.94%), Odonata larva 14.39% (Chun Ting section 12.45%, preceding original Featherlegs section 1.94), grasshopper
13.92%, Kuan Qi Lap 6.83%, ant 5.65%, Semiptera larva (Corixidae 2.23%).
The quantitative analysis of each food source component in 2 opsariichthys bidens gastrointestinal content of table
Food habits of the embodiment 3 to ominivore-fish carp
The carp sample random acquisition of the present embodiment is in Guangdong Province's Huizhou West Lake, stomach and intestine dissection and content sampling process and behaviour
Make step, calculation method with embodiment 1.Identify 2 kinds of mollusk altogether: fresh water mussel, Corbicula fluminea, a kind of water plant: black algae.
Directly eat that mode is different, and carp first bites water plant into pieces and with chlamydate mollusk from carnivorous and insect feeding fish
After eat, therefore only residual water plant and mollusk fragment in content, no complete individuals, food source composition are as shown in Figure 3.Water
The cauline leaf residuum of plant flocks together weighing after can picking under body formula anatomical lens；Mollusk casing fragments indigestibility and
It easily picks, but since its musculature is easily decomposed by gastric juice, therefore individual weight error becomes larger.Side described in specification can be taken
Method, according to the weight in wet base ratio (shell of fresh water mussel: meat ≈ 0.711, the shell of Corbicula fluminea: meat ≈ of mollusk shell and muscle parts
2.419) casing fragments of the fresh water mussel and Corbicula fluminea that are collected into, are unified into weighing respectively, then according to shell meat than calculating the two
Complete individuals weight before prey.
Other than step S1 described in embodiment 1 and step S2, the present embodiment is further comprising the steps of:
S3. a part in mixing food group j is taken to be placed in 20mm × 20mm (long × wide) glass counting plate surface, glass tally
Face is divided into 10 × 10 counting lattice (4mm2/ lattice), it is diluted and is stirred evenly with water (distilled water), pressing coverslip makes miniature
Food source generates deformation, and under dislocation optical microscopy, first full sheet counts and is greater than 50 μm of miniature food source under 10 × 10 times of mirrors, then at
Full sheet counts miniature food source less than 50 μm under 10 × 40 times of mirrors, to miniature 1~m of food source in the mixing food group j observed into
Row taxonomic identification；
Carp also scrapes aufwuch and the dislodged debris of food stromal surface, such as Fig. 4 in addition to bite mollusk and water plant
Shown in grow nonparasitically upon another plant algae group, therefore, in the stomach content sample inspection to carp, mix miniature food group and also occupy certain proportion.
S4. wait pick mollusk and aquatic plant residues, take part mixing food group in glass counting plate surface (20mm
× 20mm), it is transferred under optical microscopy, sticky mixing food group is diluted and is stirred evenly with distilled water, with coverslip pressure
Tightly to food source deformation, the percentage that each miniature food source accounts for content total amount is further calculated.
It is estimated according to the approximate geometry of all kinds of miniature food sources (rectangle, triangle, trapezoidal, oval) under the microscope
Surface area S (μm 2), due to being pressed deformation by coverslip, each miniature food source height H (μm) can be considered unanimously, when calculating volume
It can directly reduce, the approximation surface product of miniature food source j1~jm is Sj1~Sjm (μm 2), volume Vj1 in gained mixing food group j
~Vjm (μm 3) further calculates percent by volume RVj1~RVjm of content total amount shared by each miniature food source in mixing food group
(%):
Vjm(μm3)=S × H
The present embodiment carp gastrointestinal content quantitative analysis results are as shown in table 3, and Major Foods type is successively are as follows: software is dynamic
Object 80.92%, water plant 14.99%, organic debris 3.39%, phytoplankton 0.62%, zooplankter 0.08%；According to each
Food source component proportion sorts successively are as follows: fresh water mussel 80.92%, Corbicula fluminea 14.99%, black algae 11.90%, watermifoil
3.09%, article roundness 2.91%, Chlorophyta 0.48%, canebreak 0.48%, Bacillariophyta 0.10%, Copepods
0.065%, Cyanophyta 0.035%, branch pin class 0.016%.
The quantitative analysis of each food source component in 3 carp gastrointestinal content of table
Trophic analysis of the embodiment 4 to filter-feeding fish flathead
The present embodiment flathead sample random acquisition is in Guangdong Province's Zhujiang Estuary, and stomach and intestine dissection and content sampling process are the same as implementation
Example 1.Different from other fish species, flathead collects planktonic organism and particulate matter in water body with the close gill raker filter of elongated and arrangement,
Its special feeding method determines in stomach and intestine without bulk food residuum, is only made of (shown in Fig. 5) miniature mixing food source.To flathead
Miniature food source in gastrointestinal content carries out quantitative analysis, and method includes carp stomach and intestine with described in step S3 and S4 in embodiment 3
The detection and calculating of miniature food group are mixed in object.Flathead gastrointestinal content quantitative analysis results are as shown in table 4, Major Foods type
Successively are as follows: article roundness 56.14%, zooplankter 19.69%, canebreak 14.64%, phytoplankton 9.52%；According to
Each food source component proportion sequence is successively are as follows: article roundness 56.14%, canebreak 14.64%, wheel animalcule 7.91%, oar
Sufficient class 6.16%, branch pin class 5.02%, Cyanophyta 3.86%, Chlorophyta 5.06%, protozoan 0.61%, Bacillariophyta
0.58%, Chrysophyta 0.02%, Xanthophyta 0.002%.It must be noted that most of fish are in its larva and juvenile stage in addition to flathead
It can be the miniature food source of representative as object of mainly ingesting using planktonic organism, therefore, to the gastrointestinal content detection process of flathead
It is equally applicable to the fish individual of larva and juvenile and juvenile fish stage.
The quantitative analysis of each food source component in 4 flathead gastrointestinal content of table
Claims (12)
1. a kind of river fishes gastrointestinal content quick quantitative analytic method, which comprises the following steps:
S1. in anatomical isolation fish sample gastrointestinal content, taxonomic identification is carried out to its each component, judges to whether there is in stomach and intestine
Bulk food residuum or the difficult miniature food source distinguished can be debated；
If there is no bulk food residuum can be debated, it is directly entered step S3 and S4；
If there is no the miniature food source that hardly possible is distinguished, the detection of step S2 is only carried out；
It is coexisted if the miniature food source that bulk food residuum and hardly possible are distinguished can be debated, collects the miniature food source that remaining difficulty is distinguished and eaten as mixing
Group carries out the detection of step S2 to S4；
S2. the weight in wet base for weighing each component in bulk food residuum weighs the mixing food group of collection as one-component, with
Weight percent carries out Preliminary division to main food source component each in content；
S3. it takes part mixing food group to be placed under optical microscopy and carries out taxonomic identification, calculate each miniature food source volume in tally,
The miniature food source component in mixing food group is compared with volume basis to be divided；
S4. food group weight percent is mixed according to gained, restores the actual ratio of content total amount shared by each miniature food source, made complete
Portion's food source component is unified in dimension, quantitative fish gastrointestinal content.
2. fish gastrointestinal content quick quantitative analytic method according to claim 1, which is characterized in that step S1 and step
S2 is to the bulk 1~i of food residuum picked out under body formula anatomical lens into body row taxonomic identification, and is weighed to each component
W1 ~ Wi, the remaining mixing food group j that can not be recognized are accounted for by calculating W1 ~ Wi and W mixing and are included as one-component weighing W mixing
Object gross weight W total percentage obtains the relatively heavy magnitude of the 1~i of food source component identified and unidentified mixing food group j, respectively
It is calculated as RG1~RGi and RG mixing %, calculation method is as follows:
。
3. river fishes gastrointestinal content quick quantitative analytic method according to claim 1, which is characterized in that the bulk
Food residuum includes: that various fish, shell-fish necton, mollusk, aquatic and terrestrial larva, coastal waters crinosity link are dynamic
Object, aquatic borne plant debris and/or sediment humus fragment.
4. river fishes gastrointestinal content quick quantitative analytic method according to claim 2, which is characterized in that the bulk
Food residuum includes: that various fish, shell-fish necton, mollusk, aquatic and terrestrial larva, coastal waters crinosity link are dynamic
Object, aquatic borne plant debris and/or sediment humus fragment.
5. river fishes gastrointestinal content quick quantitative analytic method according to claim 1, which is characterized in that step S3 and
Step S4 is that a part of mixing food group is taken to be placed in glass counting plate surface, is diluted and is stirred evenly with water, pressing coverslip makes
Miniature food source generates deformation, and first full sheet counts the miniature food source greater than 50 μm under 10 × 10 times of mirrors, under 10 × 40 times of mirrors
Full sheet counts the miniature food source less than 50 μm, carries out taxonomic identification to miniature 1~m of food source in the mixing food group j observed.
6. river fishes gastrointestinal content quick quantitative analytic method according to claim 1, which is characterized in that in microscope
The surface area S of lower all kinds of miniature food sources of measurement, due to being pressed deformation, each miniature H(μm of food source height by coverslip) it can be considered one
Cause, in gained mixing food group j the volume of miniature food source j1~jm be Vj1~Vjm(μm 3), further calculate each in mixing food group
Percent by volume RVj1~RVjm(% of content total amount shared by miniature food source):
。
7. according to claim 1 to any one of 6 fish gastrointestinal content quick quantitative analytic methods, which is characterized in that also
Include the steps that eliminating the weight error that each food source component is generated by digestion process；
Each food source component of elimination includes: to adopt in document report or environment because of the method for the weight error that digestion process generates
Complete a counterpoise of collection, the corrigendum weight as food residuum in content；Alternatively, if in content certain class food source number of individuals compared with
More, screening mutually retains the counterpoise of complete individuals as corrigendum weight；Alternatively, part of the food residuum is difficult to weigh because figure is too small,
It first estimates its geometry, it is assumed that specific gravity is 1, its actual weight is calculated according to " volume-weight " relationship；Or
Person measures head capsule, the instep limb, circumorbital bone organ length that food source is remained in content, according to " organ length-body length-volume/weight "
Relationship calculates its actual weight；Alternatively, heavy casing fragments in content are collected, according to the weight ratio between shell and muscle
It is corrected.
8. river fishes gastrointestinal content quick quantitative analytic method according to claim 1, which is characterized in that described miniature
Food source includes: phytoplankton, zooplankter, attached algae, organic debris, suspended particulate substance and/or Sediments.
9. river fishes gastrointestinal content quick quantitative analytic method according to claim 5, which is characterized in that described miniature
Food source includes: phytoplankton, zooplankter, attached algae, organic debris, suspended particulate substance and/or Sediments.
10. river fishes gastrointestinal content quick quantitative analytic method according to claim 6, which is characterized in that described micro-
Type food source includes: phytoplankton, zooplankter, attached algae, organic debris, suspended particulate substance and/or Sediments.
11. application of any one of claims 1 to 10 method in terms of ecology of fishes Mesichthyes preferred plant species.
12. application according to claim 11, which is characterized in that determined applied to the feeding habits composition to different fingerlings
Amount is analyzed, is compared between food source difference different fingerlings, in terms of food web building or the research of ecosystem energy flow.
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| CN102250882A (en) * | 2011-06-27 | 2011-11-23 | 中国科学院南海海洋研究所 | Method for extracting DNA (Deoxyribonucleic Acid) of total genome from zooplankter and intestinal inclusions thereof |
| CN202305397U (en) * | 2011-11-04 | 2012-07-04 | 北京倍肯恒业科技发展有限责任公司 | Digital food safety fast detecting device |
| CN105483120A (en) * | 2016-02-16 | 2016-04-13 | 天津农学院 | High-temperature combined method for extracting genomic DNA of fish enteric microorganisms |
| CN205506603U (en) * | 2016-02-22 | 2016-08-24 | 张维 | Device for detecting insect feeding characteristics |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102250882A (en) * | 2011-06-27 | 2011-11-23 | 中国科学院南海海洋研究所 | Method for extracting DNA (Deoxyribonucleic Acid) of total genome from zooplankter and intestinal inclusions thereof |
| CN202305397U (en) * | 2011-11-04 | 2012-07-04 | 北京倍肯恒业科技发展有限责任公司 | Digital food safety fast detecting device |
| CN105483120A (en) * | 2016-02-16 | 2016-04-13 | 天津农学院 | High-temperature combined method for extracting genomic DNA of fish enteric microorganisms |
| CN205506603U (en) * | 2016-02-22 | 2016-08-24 | 张维 | Device for detecting insect feeding characteristics |
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| 斑点叉尾蛔和杂交鳃幼鱼昼夜摄食节律和胃肠排空时间的研究;董桂芳等;《水生生物学报》;20130930;第37卷(第5期);第876-884页 |
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