CN107144488A - A kind of fish gastrointestinal content quick quantitative analytic method and its application - Google Patents
A kind of fish gastrointestinal content quick quantitative analytic method and its application Download PDFInfo
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Abstract
The invention discloses a kind of fish gastrointestinal content quick quantitative analytic method and its application in fish nutrition ecological study.Anatomical isolation fish gastrointestinal content of the present invention, judges in stomach and intestine with the presence or absence of bulk food residuum or the difficult miniature food source distinguished；By weighing the weight in wet base of each component weight in wet base and the miniature food source of mixing in bulk food residuum respectively, each main food source component in inclusion is divided with relative weight percents；The miniature food source component in mixing food group is divided with relative volume percentage；With reference to actual measurement mixing food group percentage by weight, the actual ratio of inclusion total amount shared by each miniature food source is reduced, makes whole food source components in inclusion unified in dimension.As a result show, present method solves the influence that individual of sample size, feeding method and food source difference are analyzed feeding habits of fishes, quantitative analysis can be carried out to different water body Mesichthyes feeding habits composition, and be easy to the plasmodium cynomolgi between different each fingerlings.The inventive method has widely applicable, simple operation and other advantages, and important technological means is provided for ecology of fishes research.
Description
Technical field
The present invention relates to biological nutrition ecological study technical field, more particularly, to a kind of fish gastrointestinal content
Quick quantitative analytic method and its application.
Background technology
The standard method of modern ecology of fishes Mesichthyes preferred plant species is gastrointestinal content analytic approach, utilizes the method one
Aspect can estimate the trophic level of the trophic structure and every kind of fish of Fish Communities in group, further study ecosystem
Material circulation in system on food chain and food web, is on the other hand researching fish population consumption quantity of food, i.e. biology energy
Revenue and expenditure problem.
In existing research method, the analysis of fish gastrointestinal content can pass through the frequency of occurrences of certain class food source, number of individuals, body
Product and mass percent are determined.
The frequency of occurrences is the percentage that the stomach number containing a certain food ingredients accounts for total stomach number (non-NULL stomach number).If food kind
Class easily determines that then this method is rapid, easy.But it is only capable of roughly reflecting a side of feeding habits of fishes, i.e., fish are to certain
Plant the fancy grade of food, it is impossible to clearly express the ratio that a certain group food composition accounts for stomach inclusion quantity and volume.
Number of individuals composition is that the number of individuals of a certain group food composition in stomach inclusion accounts for food composition total number in stomach inclusion
Percentage, the method is applied to the close fish of the individual specification of detection food source, such as using planktonic organism as ichthyophagy class, eaten detecting
During the more miscellaneous fish of property, number of individuals method may exaggerate the importance of small-sized biological food source, and because small-sized biological digests rapidly,
It is difficult to accurate counting in detection.Further, since food is digested analysis in enteron aisle, single food individual may be counted repeatedly
Number, such as Annelida food source can fragment into multiple similar individuals, so as to expand the food importance of certain class bion.
Weight and volume percentage refers to that the corrigendum weight and volume of a certain group food accounts for gastrointestinal content gross weight and total
The percentage of volume, the importance of gravimetric method easily too high single big individual food ingredients of estimation in the important Journal of Sex Research of food,
Miniature organism is difficult to weigh when the inmature fish of analysis and small-sized ominivore-fish.
Volumetric method can separate food ingredients from inclusion using classification measuring appliance and be calculated volume, for being difficult to
The small-sized food individual of volume is directly calculated, then the cumulative volume of computable number food individual calculates the average external volume of individual,
Although percent by volume method can more objectively react food composition, analyzing containing the complicated shell-fish of contour structures or having heavily fortified point
During the mollusk food lumps of hard coat, volumetric method complex operation and sizeable device for volume measurement is also difficult to make.
The analysis method of existing fish stomach inclusion, and without rational versatility, can not all meet comprehensive detection water
In the ecosystem the problem of different fish food source compositions, therefore, it is badly in need of a kind of widely applicable, simple to operate fish stomach and intestine
Inclusion quick quantitative analytic method.
The content of the invention
The technical problem to be solved in the present invention is that the analysis method for being directed to existing fish stomach inclusion can not meet unified point
Analysing the technological deficiency of different waters fish foods source composition, there is provided a kind of fish gastrointestinal content quick quantitative analytic method.
Another technical problem to be solved by the present invention is that providing methods described in ecology of fishes Mesichthyes preferred plant species side
The application in face.Using the inventive method, quantitative analysis can be carried out to the feeding habits composition of different fingerlings, and be easy to different fingerlings
Food source difference is compared, and this method can be used for the researchs such as food web structure and ecosystem energy flow.
To reach above-mentioned purpose, the present invention provides a kind of river fishes gastrointestinal content quick quantitative analytic method, including
Following steps：
S1. anatomical isolation fish gastrointestinal content, taxonomic identification is carried out to its each component, and judging to whether there is in stomach and intestine can
Debate bulk food residuum or the difficult miniature food source distinguished；
If there is no bulk food residuum can be debated, step S3 and S4 are directly entered；
The miniature food source distinguished if there is no hardly possible, only carries out step S2 detection；
Coexisted if bulk food residuum and the difficult miniature food source distinguished can be debated, the miniature food source that the remaining difficulty of collection is distinguished is as mixed
Food group is closed, step S2 to S4 detection is carried out；
S2. the weight in wet base of each component in bulk food residuum is weighed, the mixing food group of collection is claimed as one-component
Weight, Preliminary division is carried out with percentage by weight to each main food source component in inclusion；
S3. take part mixing food group to be placed under light microscope and carry out taxonomic identification, each miniature food source in measuring and calculating tally
Volume, compares the miniature food source component in mixing food group with volume basis and is divided；
S4. according to gained mixing food group percentage by weight, the actual ratio of inclusion total amount shared by each miniature food source is reduced,
Make whole food source components unified in dimension, quantitative fish gastrointestinal content.
Preferably, step S1 is to split fish sample from belly to take out gastrointestinal tissue, gastrointestinal wall is cut off with dissecting scissors, with solution
Inclusion in cut open cutter and tweezers scraping stomach and intestine, retains effectively (plumpness>20%) inclusion sample, is placed in glass dish, in body
The bulk food 1~i of residuum that can be directly picked in inclusion is separated under formula anatomical lens, the remaining miniature food by that can not recognize is collected
The mixing food group j of source composition；
Step S1 and step S2 are to enter body row classification mirror to the 1~i of bulk food residuum picked out under body formula anatomical lens
It is fixed, and the W that weighs is carried out to each component1~Wi(weight in wet base g, similarly hereinafter), the mixing food group j that residue can not be recognized claims as one-component
Weight WMixing, by calculating W1~WiAnd WMixingAccount for inclusion gross weight WAlwaysPercentage, obtain the 1~i of food source component identified and do not know
Not Hun He the group of food j relatively heavy value, RG is calculated as respectively1~RGiAnd RGMixing(%), computational methods are as follows：
WAlways(g)=W1+W2…+Wi+WMixing
Step S3 is to take the part in mixing food group j to be placed in 20mm × 20mm (long × wide) glass counting plate surface, glass
Count plate face and be divided into 10 × 10 counting lattice (4mm2/ lattice), it is diluted and is stirred evenly with water (distilled water), presses cover glass
Miniature food source is set to produce under deformation, dislocation light microscope, first full sheet counts the miniature food more than 50 μm under 10 × 10 times of mirrors
Source, full sheet counts the miniature food source less than 50 μm under 10 × 40 times of mirrors, the miniature food source in couple mixing food group j observed
1~m carries out taxonomic identification；
Step S4 is (rectangle, triangle, trapezoidal, ellipse according to the approximate geometries of all kinds of miniature food sources under the microscope
Circle) estimate its surface area S (μm2), due to being pressed deformation by cover glass, each miniature food source height H (μm) can be considered consistent, in meter
Can directly it be reduced when calculating volume, miniature food source j in gained mixing food group j1~jmApproximation surface product be Sj1~Sjm(μm2), body
Product is Vj1~Vjm(μm3), further calculate the percent by volume RV of inclusion total amount shared by each miniature food source in mixing food groupj1
~RVjm(%)：
Vim(μm3)=SxH
In the inventive method, the bulk food residuum includes：Various fish, shell-fish necton, mollusk, water
Raw and terrestrial larva, coastal waters crinosity annelid, aquatic borne plant debris and/or sediment humus fragment.
Preferably, in technical scheme steps S1 and step S2 of the present invention, can directly it be picked under body formula anatomical lens
Bulk food residuum includes：Various fish, shell-fish necton, molluscan muscle and the broken shell, aquatic insect such as shrimp, crab
Larva and littoral zone terrestrial insect, it is clam worm, the extra large coastal waters such as naiad and Sabellidae crinosity annelid, aquatic borne plant debris, heavy
Product humus fragment；
Preferably, technical scheme steps S3 and step S4 of the present invention, taxonomic identification is miniature under an optical microscope
Food residuum includes：Phytoplankton (based on cyanobacteria, Chlorophyta and planktonic diatom), zooplankter (branch pin class, Copepods, wheel
Worm, protozoan), attached algae (based on grow nonparasitically upon another plant diatom and silkweed), organic debris (degrading plant, fungi, microorganism
Film), suspended particulate substance, Sediments.
Preferably, technical scheme of the present invention further relates to reduce incomplete food source weight to quilt outside step S1 to S4
The step of preying on preceding complete individuals weight and method, can preferably eliminate the error that each food source is produced by digestion process, the side
Method includes following：1) the complete individuals average weight gathered with document report or identical waters, is used as such food source in inclusion
The corrigendum weight of incomplete individual；2) class of certain in inclusion food source number of individuals more (such as Diptera, Ephemerida, Decapoda, Amphipoda
Deng), it can screen and retain relatively complete individual average weight as corrigendum weight；3) (body is long for part food residuum<3mm, such as
Oligochaetes, chironomus larvas, microcrustacean etc.) it is difficult to weigh because individual is too small, its approximate geometry can be first estimated, in vacation
Determine proportion in the case of 1, its weight is estimated according to " body length-body weight " relation；4) part food residuum distinguish by form difficulty, can be right
It remains (such as aquatic insect, shell-fish and Polychaeta) head capsule, instep limb, circumorbital bone, bristle, kiss, the length of parapodum organ and entered
Row measurement, according to " organ length-body length-volume/body weight " empirical equation, estimates weight before the food source prey；5) collect and include
Heavy mollusk casing fragments in thing, according to the weight ratio between mollusk shell and muscle, are reduced before its prey
Weight.
The beneficial effects of the present invention are：
(1) reduce tradition and include object detecting method in size of food, volume, weight, frequency of occurrences statistic processes
Complexity, simplifies taxonomic identification and quantitative analysis step.
(2) particularity of traditional single gastrointestinal content analysis method when analyzing different feeding fishes is overcome, can
To be ingested to difference, form fish carry out united analysis, and testing result has extensive comparativity.
(3) employ the method for eliminating individual weight error, will digest food residuum be restored to it is complete before prey
Individual weight, makes quantitative analysis results more accurate.
(4) gastrointestinal content that the present invention can be rapidly to fish is analyzed, and is aquatic ecosystem Mesichthyes food web
Research provide easily food habits method.
Brief description of the drawings
Fig. 1 the inventive method step schematic diagrams.
Source component identification is shared the meal in the middle part of Fig. 2 snakeheads gastrointestinal content.
Source component identification is shared the meal in the middle part of Fig. 3 opsariichthys bidenses gastrointestinal content.
Source component identification is shared the meal in the middle part of Fig. 4 carps gastrointestinal content.
Source component identification is shared the meal in the middle part of Fig. 5 flatheads gastrointestinal content.
Embodiment
Below in conjunction with the accompanying drawings the inventive method is further illustrated with specific embodiment.Following embodiments and accompanying drawing are only used for showing
Example property explanation, it is impossible to be interpreted as limitation of the present invention.Unless stated otherwise, the reagent raw material used in following embodiments is normal
The life reagent raw material that purchased in market or commercial sources are obtained is advised, unless stated otherwise, the method and apparatus used in following embodiments is
Method and apparatus commonly used in the art.The inventive method step schematic diagram is as shown in Figure 1.
Food habits of the embodiment 1 to predacious fish snakehead
The snakehead sample that the present embodiment is used is in Guangdong Province's Xinfengjiang Reservoir random acquisition.
S1. taking-up stomach and intestine are splitted from belly, gastrointestinal wall is cut off with dissecting scissors, scraped and included in stomach and intestine with scalpel and tweezers
Thing, retains 8 effective (plumpness>20%) inclusion sample, is placed in glass dish, to big in sample under body formula anatomical lens
Block food residuum is separated.
S2. to carrying out taxonomic identification to i bulk food residuum picking out under body formula anatomical lens, according to the main of food source
Morphological feature and classification organ, the present embodiment identify 4 kinds of fish altogether：Sub- Ling Wen Acenthogobius flavimanus, Kuan Qi Lap, striped two must Barb, mud
Loach；1 kind of shell-fish：Macrobrachium nipponensis；The section of aquatic insect larva 2：Chun Ting sections and Cordulegasteridae, food source composition are as shown in Figure 2.From food
Judge in integrality, the prey time of fish and shrimp is shorter, individual is relatively complete, can directly weigh, and Odonata larva limbs
It is broken, have heavy head capsule (including mouthpart) only and instep limb is also more complete, it is completely individual according to Chun Ting sections and Cordulegasteridae larva
" instep limb length-body weight " relation of body, the individual weight before its prey is calculated with the instep limb remained in inclusion.In addition, because of meat
Feeding fish is not ingested directly miniature food source, and the thick mixing food group microscopy wrapped up under an optical microscope remaining gastric juice
Any food sign is not found, therefore ignores mixing food group proportion.It is 0.0001g's in sensibility reciprocal in units of individual of sample
Each food source weight in wet base W identified is weighed on electronic balance1~W6(g) its relative weight percents RG, is calculated1~RG6。
Computational methods are as follows：
WAlways(g)=W1+W2…+Wi+WMixing
As shown in table 1, its Major Foods type is followed successively by snakehead gastrointestinal content quantitative analysis results：Fish 75.53%, shrimp
19.71%th, dragonfly larva 4.75%；It is followed successively by according to the sequence of each food source component proportion：Sub- Ling Wen Acenthogobius flavimanus 31.91%,
Striped two must Barb 20.99%, Macrobrachium nipponensis 19.71%, wide fin Lap 14.87%, loach 7.76%, Chun Ting sections 2.42%, big dragonfly
Section 2.33%.
The quantitative analysis results of each food source component in the snakehead gastrointestinal content of table 1
Food habits of the embodiment 2 to insect feeding fish opsariichthys bidenses
The present embodiment opsariichthys bidenses sample random acquisition is in Guangdong Province Liu Xihe, stomach and intestine dissection and inclusion sampling process and behaviour
Make step, computational methods be the same as Example 1.The section of aquatic insect larva 6 is identified altogether：Hydropsychidae, multiple spurs phryganeid section, four section Fu
Section, flat Fu sections, Chun Ting sections, Corixidae；2 kinds of terrestrial insect：Grasshopper, ant；1 kind of fish：Kuan Qi Lap (juvenile fish).With carnivorous fish
The fish of big individual, shrimp food source are different in class stomach, many based on aquatic insect larva in insect feeding fish gastrointestinal content, food source
Though individual is small, quantity is more, in addition to the computational methods be the same as Example 1 of Chun Ting sections residuum, and the weight of other small individual insects is calculated,
Take and retain relatively complete insect individual in inclusion, the complete individuals before prey are represented with these individual Weight averages
Weight.In addition, the part that spectroscopy is found under insect feeding fish also less miniature food source of directly ingesting, light microscope is grown nonparasitically upon another plant
Taken in when algae and canebreak is episites, therefore as predacious fish, ignore institute of a residual mixing food group accounting in stomach and intestine
Example.Opsariichthys bidenses food source is constituted as shown in figure 3, gastrointestinal content quantitative analysis results are as shown in table 2, and its Major Foods type is successively
For：Aquatic insect 73.60%, terrestrial insect 19.56%, fish 6.83%；It is followed successively by according to the sequence of each food source component proportion：
Trichoptera larva 31.47% (hydropsychidae 16.60%, multiple spurs phryganeid section 14.87%), (the four section Fu of Ephemerida larva 25.52%
Section 17.58%, flat Fu sections 7.94%), Odonata larva 14.39% (Chun Ting sections 12.45%, preceding former Featherlegs sections 1.94), grasshopper
13.92%th, Kuan Qi Lap 6.83%, ant 5.65%, Semiptera larva (Corixidae 2.23%).
The quantitative analysis of each food source component in the opsariichthys bidenses gastrointestinal content of table 2
Food habits of the embodiment 3 to ominivore-fish carp
The carp sample random acquisition of the present embodiment is in Guangdong Province's Huizhou West Lake, stomach and intestine dissection and inclusion sampling process and behaviour
Make step, computational methods be the same as Example 1.2 kinds of mollusk is identified altogether：Fresh water mussel, Corbicula fluminea, a kind of water plant：Black algae.
Directly eat that mode is different from carnivorous and insect feeding fish, carp first bites water plant into pieces and with chlamydate mollusk
After eat, therefore only residual water plant and mollusk fragment in inclusion, no complete individuals, food source composition is as shown in Figure 3.Water
The cauline leaf residuum of plant flocks together after being picked under body formula anatomical lens and weighed；Mollusk casing fragments indigestibility and
Easily pick, but because its musculature is easily decomposed by gastric juice, therefore individual weight error becomes big.Side described in specification can be taken
Method, according to mollusk shell and the weight in wet base ratio (shell of fresh water mussel of muscle parts:Meat ≈ 0.711, the shell of Corbicula fluminea:Meat ≈
2.419), the fresh water mussel being collected into and the casing fragments of Corbicula fluminea are uniformly weighed respectively, then according to shell meat than calculating the two
Complete individuals weight before prey.
Beyond the step S1 and step S2 described in embodiment 1, the present embodiment is further comprising the steps of：
S3. the part in mixing food group j is taken to be placed in 20mm × 20mm (long × wide) glass counting plate surface, glass tally
Face is divided into 10 × 10 and counts lattice (4mm2/ lattice), it is diluted and is stirred evenly with water (distilled water), pressing cover glass makes miniature
Food source is produced under deformation, dislocation light microscope, and first full sheet counts the miniature food source more than 50 μm under 10 × 10 times of mirrors, then at
Full sheet counts the miniature food source less than 50 μm under 10 × 40 times of mirrors, and miniature 1~m of food source in couple mixing food group j observed enters
Row taxonomic identification；
Carp also scrapes aufwuch and the dislodged debris of food stromal surface, such as Fig. 4 in addition to food mollusk and water plant is stung
Shown in grow nonparasitically upon another plant algae group, therefore, in being examined to the stomach inclusion sample of carp, mix miniature food group and also occupy certain proportion.
S4. wait to have picked mollusk and aquatic plant residues, take part mixing food group in glass counting plate surface (20mm
× 20mm), it is transferred under light microscope, sticky mixing food group is diluted and stirred evenly with distilled water, cover glass pressure is used
Tightly to food source deformation, the percentage that each miniature food source accounts for inclusion total amount is further calculated.
It is estimated according to the approximate geometry (rectangle, triangle, trapezoidal, ellipse) of all kinds of miniature food sources under the microscope
Surface area S (μm 2), due to being pressed deformation by cover glass, each miniature food source height H (μm) can be considered consistent, when calculating volume
Can directly it reduce, miniature food source j1~jm approximation surface product is Sj1~Sjm (μm 2) in gained mixing food group j, and volume is Vj1
~Vjm (μm 3), further calculates percent by volume RVj1~RVjm of inclusion total amount shared by each miniature food source in mixing food group
(%)：
Vjm(μm3)=S × H
As shown in table 3, its Major Foods type is followed successively by the present embodiment carp gastrointestinal content quantitative analysis results：Software is moved
Thing 80.92%, water plant 14.99%, organic debris 3.39%, phytoplankton 0.62%, zooplankter 0.08%；According to each
The sequence of food source component proportion is followed successively by：Fresh water mussel 80.92%, Corbicula fluminea 14.99%, black algae 11.90%, watermifoil
3.09%th, article roundness 2.91%, Chlorophyta 0.48%, canebreak 0.48%, Bacillariophyta 0.10%, Copepods
0.065%th, Cyanophyta 0.035%, branch pin class 0.016%.
The quantitative analysis of each food source component in the carp gastrointestinal content of table 3
Trophic analysis of the embodiment 4 to filter-feeding fish flathead
The present embodiment flathead sample random acquisition is in Guangdong Province's Zhujiang Estuary, and stomach and intestine dissection and inclusion sampling process are with implementation
Example 1.Different from other fish species, flathead collects planktonic organism and particulate matter in water body with the close gill raker filter of elongated and arrangement,
Its special feeding method is determined without bulk food residuum in stomach and intestine, is only made up of (shown in Fig. 5) miniature mixing food source.To flathead
Miniature food source in gastrointestinal content carp stomach and intestine are included described in step S3 and S4 in quantitative analysis, method be the same as Example 3
The detection and calculating of miniature food group are mixed in thing.Flathead gastrointestinal content quantitative analysis results are as shown in table 4, its Major Foods type
It is followed successively by：Article roundness 56.14%, zooplankter 19.69%, canebreak 14.64%, phytoplankton 9.52%；According to
Each food source component proportion sequence is followed successively by：Article roundness 56.14%, canebreak 14.64%, wheel animalcule 7.91%, oar
Sufficient class 6.16%, branch pin class 5.02%, Cyanophyta 3.86%, Chlorophyta 5.06%, protozoan 0.61%, Bacillariophyta
0.58%th, Chrysophyta 0.02%, Xanthophyta 0.002%.It must be noted that in addition to flathead, most of fish are in its larva and juvenile stage
Miniature food source that can be by representative of planktonic organism is as main object of ingesting, therefore, to the gastrointestinal content detection process of flathead
It is equally applicable to larva and juvenile and the fish individual of juvenile fish stage.
The quantitative analysis of each food source component in the flathead gastrointestinal content of table 4
Claims (9)
1. a kind of river fishes gastrointestinal content quick quantitative analytic method, it is characterised in that comprise the following steps：
S1. in anatomical isolation fish sample gastrointestinal content, taxonomic identification is carried out to its each component, judges whether deposited in stomach and intestine
Bulk food residuum or the difficult miniature food source distinguished can debated；
If there is no bulk food residuum can be debated, step S3 and S4 are directly entered；
The miniature food source distinguished if there is no hardly possible, only carries out step S2 detection；
Coexisted if bulk food residuum and the difficult miniature food source distinguished can be debated, the miniature food source that the remaining difficulty of collection is distinguished is as mixing food
Group, carries out step S2 to S4 detection；
S2. the weight in wet base of each component in bulk food residuum is weighed, the mixing food group of collection is weighed as one-component,
Preliminary division is carried out to each main food source component in inclusion with percentage by weight；
S3. take part mixing food group to be placed under light microscope and carry out taxonomic identification, each miniature food source body in measuring and calculating tally
Product, compares the miniature food source component in mixing food group with volume basis and is divided；
S4. according to gained mixing food group percentage by weight, the actual ratio of inclusion total amount shared by each miniature food source is reduced, is made
Whole food source components are unified in dimension, quantitative fish gastrointestinal content.
2. fish gastrointestinal content quick quantitative analytic method according to claim 1, it is characterised in that step S1 and step
S2 be under body formula anatomical lens to pick out bulk food residuum 1~iEnter body row taxonomic identification, and each component is weighedW 1~W i, the mixing food group that residue can not be recognizedjWeighed as one-componentW Mixing, by calculatingW 1~W iWithW MixingAccount for inclusion total
WeightW AlwaysPercentage, obtain the food source component 1 that has identified~iWith unidentified mixing food groupj Relatively heavy value, count respectively
ForRG 1~RG iWithRG Mixing%, computational methods are as follows：
。
3. river fishes gastrointestinal content quick quantitative analytic method according to claim 1 or claim 2, it is characterised in that described
Bulk food residuum includes：Various fish, shell-fish necton, mollusk, aquatic and terrestrial larva, coastal waters crinosity link
Animal, aquatic borne plant debris and/or sediment humus fragment.
4. river fishes gastrointestinal content quick quantitative analytic method according to claim 1, it is characterised in that step S3 and
Step S4 is that the part for taking mixing food group is placed in glass counting plate surface, is diluted and stirred evenly with water, pressing cover glass makes
Miniature food source produces deformation, and first full sheet counts the miniature food source more than 50 μm under 10 × 10 times of mirrors, under 10 × 40 times of mirrors
Full sheet counts the miniature food source less than 50 μm, pair mixing observed food groupjIn miniature food source 1~mCarry out taxonomic identification.
5. river fishes gastrointestinal content quick quantitative analytic method according to claim 1, it is characterised in that in microscope
The approximation surface product of lower all kinds of miniature food sources of measurementS, due to being pressed deformation, each miniature food source height by cover glassH（μm）Visually
For consistent, gained mixing food groupjIn miniature food sourcej 1~j mApproximate volumes beVJ 1~Vj m（μm3）, further calculate mixing
The percent by volume of inclusion total amount shared by each miniature food source in food groupRVJ 1~RVj m（%）：
。
6. according to any one of claim 1 to the 5 fish gastrointestinal content quick quantitative analytic method, it is characterised in that also
The step of including eliminating weight error that each food source component is produced by digestion process；
The method for eliminating the weight error that each food source component is produced by digestion process includes：To be adopted in document report or environment
Complete counterpoise of collection, is used as the corrigendum weight of food residuum in inclusion；Or, if the class food source number of individuals of certain in inclusion compared with
Many, screening mutually retains the counterpoise of complete individuals as corrigendum weight；Or, part food residuum is difficult to weigh because build is too small,
Its approximate geometry is first estimated, in the case of it is assumed that proportion is 1, its actual weight is calculated according to " volume-body weight " relation；
Or, the organ length such as head capsule, instep limb, circumorbital bone of residual food source in measurement inclusion, according to " organ length-body length-volume/
Body weight " relation calculates its actual weight；Or, heavy casing fragments in inclusion are collected, according between shell and muscle
Weight is than being corrected.
7. the river fishes gastrointestinal content quick quantitative analytic method according to claim 1,4 or 5, it is characterised in that institute
Stating miniature food source includes：Phytoplankton, zooplankter, attached algae, organic debris, suspended particulate substance and/or Sediments.
8. application of any one of claim 1 to 7 method in terms of ecology of fishes Mesichthyes preferred plant species.
9. application according to claim 8, it is characterised in that be to be applied to quantify the feeding habits composition of different fingerlings
Analyze, food source difference different fingerlings is compared, food web is built or ecosystem energy flow research aspect.
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| US20070078300A1 (en) * | 2005-09-30 | 2007-04-05 | Ofra Zinaty | System and method for detecting content in-vivo |
| US20140106393A1 (en) * | 2009-02-06 | 2014-04-17 | California Institute Of Technology | Methods and systems for detection of stoichiometry by forster resonance energy transfer |
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