CN107141358A - A kind of buckwheat starch extracting method - Google Patents
A kind of buckwheat starch extracting method Download PDFInfo
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- CN107141358A CN107141358A CN201710472095.0A CN201710472095A CN107141358A CN 107141358 A CN107141358 A CN 107141358A CN 201710472095 A CN201710472095 A CN 201710472095A CN 107141358 A CN107141358 A CN 107141358A
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- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B30/00—Preparation of starch, degraded or non-chemically modified starch, amylose, or amylopectin
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- C08B30/042—Extraction or purification from cereals or grains
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Abstract
The present invention relates to a kind of technical process of processing of agriculture product, especially a kind of method that buckwheat starch is extracted from buckwheat.The present invention by being dissolved after buckwheat is ground with ethanol after water bath sonicator extract separation method separation and Extraction buckwheat in flavones；Water bath sonicator extracts the protein in the method separation and Extraction buckwheat of separation after being dissolved with sodium hydroxide solution；The method alternately cleaned with 1.0mol/L hydrochloric acid and 0.5mol/L NaOH solution removes the impurity in buckwheat starch.Flavones contained by buckwheat, protein and starch are distinguished into separation and Extraction, and possess high recovery rate, the utilization rate of buckwheat is greatly improved, reduces material waste.
Description
Technical field
Buckwheat starch is extracted from buckwheat the present invention relates to a kind of technical process of processing of agriculture product, especially one kind
Method.
Background technology
Buckwheat (scientific name：Fagopyrum esculentum Moench.), alias：Sweet tea buckwheat, black wheat, triangle wheat etc.；1 year
Sward sheet.Stem is upright, high 30-90 centimetres, top branch, green or red, has a vertical rib, hairless or along vertical rib have nipple in side
Shape projection.Leaf triangle or ovate triangle, long 2.5-7 centimetres, wide 2-5 centimetres, top is tapering, and base portion is heart-shaped, and two sides is along leaf
Arteries and veins has mastoid process.
The cultivated area and yield of China's buckwheat occupy the second in the world, and buckwheat has abundant nutritional quality.Surveyed according to expert
It is fixed, buckwheat 2%-3% containing flavones, protein 10%-18%, cellulose 10%-16%, starch 70% or so, and fat content
But it is only 2% or so.Buckwheat is also containing several mineral materials and citric acid, oxalic acid, malic acid such as abundant phosphorus, iron, calcium, magnesium, copper
Deng organic acid and vitamin C, B, B2, E, B5 etc., and content is generally higher than wheat flour, rice, sorghum flour, maize flour and small
Rice.Buckwheat has higher medicinal and health value.Buckwheat medicine spectrality is wide, evident in efficacy, pleasant, digestive with wide intestines
Function, it is all effective in cure to dysentery, cough, poisoning, burn etc.:Clinically treating hypertension, control diabetes with buckwheat has significantly
Curative effect.Also stomach invigorating, immune, anti-inflammatory, the function of anti-canceration.Buckwheat is a kind of health food to eat for medicine.Buckwheat is used as one
Kind nutritious, healthcare function is protruded and the broader coarse cereals of resource, for many years simply by buckwheat merely be processed into some foods
Product, such as：Bean jelly, thin pancake etc..And the much starch contained by itself is not really extracted and made full use of, and it is right
It is also less in the research report of buckwheat starch, show according to the study, buckwheat starch is that the buckwheat shuttle methyl starch that raw material is made is suitable to
Soup easy to make, can replace import converted starch special at present, what is be made of it facilitates soup to have first quality effect, it is answered
Water speed is better than starch special partially.
Contain substantial amounts of flavones, protein and starch in buckwheat, at present, both at home and abroad primarily directed to single substance in buckwheat
Or the extraction of two kinds of materials, comprehensive development and utilization is not carried out to flavones, protein and starch three, buckwheat resource is result in
Waste, economic benefit is high.As CN105254772A discloses a kind of production method of buckwheat starch, buckwheat starch is overcome
The problem of albumen can not be efficiently separated with starch in production process, but do not refer to and separation and Extraction, CN1654479 are carried out to flavones
Disclose the technique that a kind of levigation method continuously produces buckwheat starch, buck wheat protein, flavones, dietary fiber, but its extracting flavonoids
Rate is less than 10%, and protein extracting ratio is less than 50%.
The content of the invention
In order to solve above-mentioned technical problem present in prior art, the present invention provides a kind of buckwheat starch extracting method,
The flavones contained by buckwheat and protein are distinguished into separation and Extraction in the extraction process of buckwheat starch, and possess high extraction
Rate, greatly improves the utilization rate of buckwheat, reduces material waste, and buckwheat starch extracting method of the present invention possesses as follows
Step：
(1) buckwheat cleaned out is weighed, 4-8h is soaked with 2-3 times of water.The conventional volume of current ultrasonic extraction tank exists
30m2Hereinafter, actual buckwheat should be selected from amount according to actual place and mass flow.
(2) soaked buckwheat is fallen into soaking water, cleaning adds the weight such as the buckwheat that is weighed with step (1) after draining
Water, grind to form slurry.It is preferred that can use Buddha's warrior attendant be sanded be ground.
(3) sub-sieve for being 0.149mm with sieve aperture filters out wheat bran, filters out fine fibre for 0.088 sub-sieve with sieve aperture, obtains
To buckwheat slurry.
(4) the 70%-90% ethanol solutions of 15-25 times of weight, the water bath sonicator at 25 DEG C -50 DEG C are added in being starched to buckwheat
Extract 2-4h.It is preferred that scheme be：80% ethanol solution of 20 times of weight is added in being starched to buckwheat, water bath sonicator is carried at 35 DEG C
Take 2.5h.Strengthen extraction separation process using ultrasonic technology, extraction separation rate can be effectively improved, shorten extraction time, section
About cost, can also even improve the quality and yield of product.
(5) the buckwheat slurry 4000r/min centrifugation 15min after water bath sonicator is extracted, collect supernatant a and precipitation respectively
b。
(6) supernatant a concentrations are evaporated, obtain flavones.
(7) deionized water of 15-25 times of weight is added into precipitation b, PH to 8- is adjusted with 0.1mol/L NaOH solution
After 10 in 45 DEG C -60 DEG C water-bath ultrasonic extraction 1-5h, obtain no flavones buckwheat precipitated liquid.It is preferred that scheme be：Into precipitation b
The deionized water of 20 times of weight is added, the water-bath ultrasonic extraction 2h in 55 DEG C is adjusted after PH to 9 with 0.1mol/L NaOH solution.
Strengthen extraction separation process using ultrasonic technology, can effectively improve extraction separation rate, shorten extraction time, it is cost-effective,
The quality and yield of product even can also be improved.Extraction rate of protein can be increased by improving bath temperature, but too high temperature
Degree can cause protein denaturation, so bath temperature should be controlled at≤60 DEG C.
(8) 15min is centrifuged without flavones buckwheat precipitated liquid 4000r/min after water bath sonicator is extracted, is collected respectively
Clear liquid c and precipitation d；
(9) supernatant c concentrations are evaporated, obtain buck wheat protein；
(10) NaOH solution cyclic washings 3-5 time of the d with 1.0mol/L hydrochloric acid and 0.5mol/L will be precipitated, is then washed
To neutral, buckwheat starch is obtained after dehydrating.
It is preferred that step (6), the concentration in step (9) be evaporated the side that vacuum distillation and freeze-drying can be used to be combined
Method sloughs wherein moisture content, can so keep the activity of beneficiating ingredient not to be damaged to greatest extent.It is de- in step (10)
Water drying can use the method for freeze-drying can keep the activity of beneficiating ingredient to greatest extent not to slough wherein moisture content
It is damaged.
Compared with prior art, the technique effect of the invention is embodied in：
The present invention by being dissolved after buckwheat is ground with ethanol after water bath sonicator extract separation method separation and Extraction buckwheat
In flavones；Water bath sonicator extracts the protein in the method separation and Extraction buckwheat of separation after being dissolved with sodium hydroxide solution；With
The method that 1.0mol/L hydrochloric acid and 0.5mol/L NaOH solution are alternately cleaned removes the impurity in buckwheat starch.By buckwheat institute
Flavones, protein and the starch difference separation and Extraction contained, and possess high recovery rate, greatly improve the utilization of buckwheat
Rate, reduces material waste.
Embodiment
Limited with reference to specific embodiment technical scheme is further, but claimed
Scope is not only limited to made description.
Embodiment 1
A kind of buckwheat starch extracting method：
(1) the buckwheat 500kg cleaned out is weighed, 6h is soaked with the water of 3 times of weight.
(2) soaked buckwheat is fallen into soaking water, cleaning adds the weight such as the buckwheat that is weighed with step (1) after draining
Water, grind to form slurry.It is preferred that can use Buddha's warrior attendant be sanded be ground.
(3) sub-sieve for being 0.149mm with sieve aperture filters out wheat bran, filters out fine fibre for 0.088 sub-sieve with sieve aperture, obtains
To buckwheat slurry.
(4) 80% ethanol solution of 20 times of weight is added in being starched to buckwheat, water bath sonicator extracts 2.5h at 35 DEG C.
(5) the buckwheat slurry 4000r/min centrifugation 15min after water bath sonicator is extracted, collect supernatant a and precipitation respectively
b。
(6) supernatant a vacuum distillations are concentrated, is freeze-dried into powder, obtains flavones.
(7) add the deionized waters of 20 times of weight into precipitation b, with 0.1mol/L NaOH solution adjust after PH to 9
Water-bath ultrasonic extraction 2h obtains no flavones buckwheat precipitated liquid in 55 DEG C.
(8) 15min is centrifuged without flavones buckwheat precipitated liquid 4000r/min after water bath sonicator is extracted, is collected respectively
Clear liquid c and precipitation d；
(9) supernatant c vacuum distillations are concentrated, is freeze-dried into powder, obtains buck wheat protein；
(10) NaOH solution cyclic washings 4 time of the d with 1.0mol/L hydrochloric acid and 0.5mol/L will be precipitated, is then washed to
Neutrality, is freeze-dried into after powder and obtains buckwheat starch.
Embodiment 2
A kind of buckwheat starch extracting method：
(1) the buckwheat 500kg cleaned out is weighed, 6h is soaked with the water of 3 times of weight.
(2) soaked buckwheat is fallen into soaking water, cleaning adds the weight such as the buckwheat that is weighed with step (1) after draining
Water, grind to form slurry.It is preferred that can use Buddha's warrior attendant be sanded be ground.
(3) sub-sieve for being 0.149mm with sieve aperture filters out wheat bran, filters out fine fibre for 0.088 sub-sieve with sieve aperture, obtains
To buckwheat slurry.
(4) 80% ethanol solution of 15 times of weight is added in being starched to buckwheat, water bath sonicator extracts 2.5h at 35 DEG C.
(5) the buckwheat slurry 4000r/min centrifugation 15min after water bath sonicator is extracted, collect supernatant a and precipitation respectively
b。
(6) supernatant a vacuum distillations are concentrated, is freeze-dried into powder, obtains flavones.
(7) add the deionized waters of 20 times of weight into precipitation b, with 0.1mol/L NaOH solution adjust after PH to 9
Water-bath ultrasonic extraction 2h obtains no flavones buckwheat precipitated liquid in 55 DEG C.
(8) 15min is centrifuged without flavones buckwheat precipitated liquid 4000r/min after water bath sonicator is extracted, is collected respectively
Clear liquid c and precipitation d；
(9) supernatant c vacuum distillations are concentrated, is freeze-dried into powder, obtains buck wheat protein；
(10) NaOH solution cyclic washings 4 time of the d with 1.0mol/L hydrochloric acid and 0.5mol/L will be precipitated, is then washed to
Neutrality, is freeze-dried into after powder and obtains buckwheat starch.
Embodiment 3
A kind of buckwheat starch extracting method：
(1) the buckwheat 500kg cleaned out is weighed, 6h is soaked with the water of 3 times of weight.
(2) soaked buckwheat is fallen into soaking water, cleaning adds the weight such as the buckwheat that is weighed with step (1) after draining
Water, grind to form slurry.It is preferred that can use Buddha's warrior attendant be sanded be ground.
(3) sub-sieve for being 0.149mm with sieve aperture filters out wheat bran, filters out fine fibre for 0.088 sub-sieve with sieve aperture, obtains
To buckwheat slurry.
(4) 80% ethanol solution of 25 times of weight is added in being starched to buckwheat, water bath sonicator extracts 2.5h at 35 DEG C.
(5) the buckwheat slurry 4000r/min centrifugation 15min after water bath sonicator is extracted, collect supernatant a and precipitation respectively
b。
(6) supernatant a vacuum distillations are concentrated, is freeze-dried into powder, obtains flavones.
(7) add the deionized waters of 20 times of weight into precipitation b, with 0.1mol/L NaOH solution adjust after PH to 9
Water-bath ultrasonic extraction 2h obtains no flavones buckwheat precipitated liquid in 55 DEG C.
(8) 15min is centrifuged without flavones buckwheat precipitated liquid 4000r/min after water bath sonicator is extracted, is collected respectively
Clear liquid c and precipitation d；
(9) supernatant c vacuum distillations are concentrated, is freeze-dried into powder, obtains buck wheat protein；
(10) NaOH solution cyclic washings 4 time of the d with 1.0mol/L hydrochloric acid and 0.5mol/L will be precipitated, is then washed to
Neutrality, is freeze-dried into after powder and obtains buckwheat starch.
Embodiment 4
A kind of buckwheat starch extracting method：
(1) the buckwheat 500kg cleaned out is weighed, 6h is soaked with the water of 3 times of weight.
(2) soaked buckwheat is fallen into soaking water, cleaning adds the weight such as the buckwheat that is weighed with step (1) after draining
Water, grind to form slurry.It is preferred that can use Buddha's warrior attendant be sanded be ground.
(3) sub-sieve for being 0.149mm with sieve aperture filters out wheat bran, filters out fine fibre for 0.088 sub-sieve with sieve aperture, obtains
To buckwheat slurry.
(4) 70% ethanol solution of 20 times of weight is added in being starched to buckwheat, water bath sonicator extracts 2.5h at 35 DEG C.
(5) the buckwheat slurry 4000r/min centrifugation 15min after water bath sonicator is extracted, collect supernatant a and precipitation respectively
b。
(6) supernatant a vacuum distillations are concentrated, is freeze-dried into powder, obtains flavones.
(7) add the deionized waters of 20 times of weight into precipitation b, with 0.1mol/L NaOH solution adjust after PH to 9
Water-bath ultrasonic extraction 2h obtains no flavones buckwheat precipitated liquid in 55 DEG C.
(8) 15min is centrifuged without flavones buckwheat precipitated liquid 4000r/min after water bath sonicator is extracted, is collected respectively
Clear liquid c and precipitation d；
(9) supernatant c vacuum distillations are concentrated, is freeze-dried into powder, obtains buck wheat protein；
(10) NaOH solution cyclic washings 4 time of the d with 1.0mol/L hydrochloric acid and 0.5mol/L will be precipitated, is then washed to
Neutrality, is freeze-dried into after powder and obtains buckwheat starch.
Embodiment 5
A kind of buckwheat starch extracting method：
(1) the buckwheat 500kg cleaned out is weighed, 6h is soaked with the water of 3 times of weight.
(2) soaked buckwheat is fallen into soaking water, cleaning adds the weight such as the buckwheat that is weighed with step (1) after draining
Water, grind to form slurry.It is preferred that can use Buddha's warrior attendant be sanded be ground.
(3) sub-sieve for being 0.149mm with sieve aperture filters out wheat bran, filters out fine fibre for 0.088 sub-sieve with sieve aperture, obtains
To buckwheat slurry.
(4) 90% ethanol solution of 20 times of weight is added in being starched to buckwheat, water bath sonicator extracts 2.5h at 35 DEG C.
(5) the buckwheat slurry 4000r/min centrifugation 15min after water bath sonicator is extracted, collect supernatant a and precipitation respectively
b。
(6) supernatant a vacuum distillations are concentrated, is freeze-dried into powder, obtains flavones.
(7) add the deionized waters of 20 times of weight into precipitation b, with 0.1mol/L NaOH solution adjust after PH to 9
Water-bath ultrasonic extraction 2h obtains no flavones buckwheat precipitated liquid in 55 DEG C.
(8) 15min is centrifuged without flavones buckwheat precipitated liquid 4000r/min after water bath sonicator is extracted, is collected respectively
Clear liquid c and precipitation d；
(9) supernatant c vacuum distillations are concentrated, is freeze-dried into powder, obtains buck wheat protein；
(10) NaOH solution cyclic washings 4 time of the d with 1.0mol/L hydrochloric acid and 0.5mol/L will be precipitated, is then washed to
Neutrality, is freeze-dried into after powder and obtains buckwheat starch.
Embodiment 6
A kind of buckwheat starch extracting method：
(1) the buckwheat 500kg cleaned out is weighed, 6h is soaked with the water of 3 times of weight.
(2) soaked buckwheat is fallen into soaking water, cleaning adds the weight such as the buckwheat that is weighed with step (1) after draining
Water, grind to form slurry.It is preferred that can use Buddha's warrior attendant be sanded be ground.
(3) sub-sieve for being 0.149mm with sieve aperture filters out wheat bran, filters out fine fibre for 0.088 sub-sieve with sieve aperture, obtains
To buckwheat slurry.
(4) 80% ethanol solution of 20 times of weight is added in being starched to buckwheat, water bath sonicator extracts 2.5h at 25 DEG C.
(5) the buckwheat slurry 4000r/min centrifugation 15min after water bath sonicator is extracted, collect supernatant a and precipitation respectively
b。
(6) supernatant a vacuum distillations are concentrated, is freeze-dried into powder, obtains flavones.
(7) add the deionized waters of 20 times of weight into precipitation b, with 0.1mol/L NaOH solution adjust after PH to 9
Water-bath ultrasonic extraction 2h obtains no flavones buckwheat precipitated liquid in 55 DEG C.
(8) 15min is centrifuged without flavones buckwheat precipitated liquid 4000r/min after water bath sonicator is extracted, is collected respectively
Clear liquid c and precipitation d；
(9) supernatant c vacuum distillations are concentrated, is freeze-dried into powder, obtains buck wheat protein；
(10) NaOH solution cyclic washings 4 time of the d with 1.0mol/L hydrochloric acid and 0.5mol/L will be precipitated, is then washed to
Neutrality, is freeze-dried into after powder and obtains buckwheat starch.
Embodiment 7
A kind of buckwheat starch extracting method：
(1) the buckwheat 500kg cleaned out is weighed, 6h is soaked with the water of 3 times of weight.
(2) soaked buckwheat is fallen into soaking water, cleaning adds the weight such as the buckwheat that is weighed with step (1) after draining
Water, grind to form slurry.It is preferred that can use Buddha's warrior attendant be sanded be ground.
(3) sub-sieve for being 0.149mm with sieve aperture filters out wheat bran, filters out fine fibre for 0.088 sub-sieve with sieve aperture, obtains
To buckwheat slurry.
(4) 80% ethanol solution of 20 times of weight is added in being starched to buckwheat, water bath sonicator extracts 2.5h at 50 DEG C.
(5) the buckwheat slurry 4000r/min centrifugation 15min after water bath sonicator is extracted, collect supernatant a and precipitation respectively
b。
(6) supernatant a vacuum distillations are concentrated, is freeze-dried into powder, obtains flavones.
(7) add the deionized waters of 20 times of weight into precipitation b, with 0.1mol/L NaOH solution adjust after PH to 9
Water-bath ultrasonic extraction 2h obtains no flavones buckwheat precipitated liquid in 55 DEG C.
(8) 15min is centrifuged without flavones buckwheat precipitated liquid 4000r/min after water bath sonicator is extracted, is collected respectively
Clear liquid c and precipitation d；
(9) supernatant c vacuum distillations are concentrated, is freeze-dried into powder, obtains buck wheat protein；
(10) NaOH solution cyclic washings 4 time of the d with 1.0mol/L hydrochloric acid and 0.5mol/L will be precipitated, is then washed to
Neutrality, is freeze-dried into after powder and obtains buckwheat starch.
Embodiment 8
A kind of buckwheat starch extracting method：
(1) the buckwheat 500kg cleaned out is weighed, 6h is soaked with the water of 3 times of weight.
(2) soaked buckwheat is fallen into soaking water, cleaning adds the weight such as the buckwheat that is weighed with step (1) after draining
Water, grind to form slurry.It is preferred that can use Buddha's warrior attendant be sanded be ground.
(3) sub-sieve for being 0.149mm with sieve aperture filters out wheat bran, filters out fine fibre for 0.088 sub-sieve with sieve aperture, obtains
To buckwheat slurry.
(4) 80% ethanol solution of 20 times of weight is added in being starched to buckwheat, water bath sonicator extracts 2h at 35 DEG C.
(5) the buckwheat slurry 4000r/min centrifugation 15min after water bath sonicator is extracted, collect supernatant a and precipitation respectively
b。
(6) supernatant a vacuum distillations are concentrated, is freeze-dried into powder, obtains flavones.
(7) add the deionized waters of 20 times of weight into precipitation b, with 0.1mol/L NaOH solution adjust after PH to 9
Water-bath ultrasonic extraction 2h obtains no flavones buckwheat precipitated liquid in 55 DEG C.
(8) 15min is centrifuged without flavones buckwheat precipitated liquid 4000r/min after water bath sonicator is extracted, is collected respectively
Clear liquid c and precipitation d；
(9) supernatant c vacuum distillations are concentrated, is freeze-dried into powder, obtains buck wheat protein；
(10) NaOH solution cyclic washings 4 time of the d with 1.0mol/L hydrochloric acid and 0.5mol/L will be precipitated, is then washed to
Neutrality, is freeze-dried into after powder and obtains buckwheat starch.
Embodiment 9
A kind of buckwheat starch extracting method：
(1) the buckwheat 500kg cleaned out is weighed, 6h is soaked with the water of 3 times of weight.
(2) soaked buckwheat is fallen into soaking water, cleaning adds the weight such as the buckwheat that is weighed with step (1) after draining
Water, grind to form slurry.It is preferred that can use Buddha's warrior attendant be sanded be ground.
(3) sub-sieve for being 0.149mm with sieve aperture filters out wheat bran, filters out fine fibre for 0.088 sub-sieve with sieve aperture, obtains
To buckwheat slurry.
(4) 80% ethanol solution of 20 times of weight is added in being starched to buckwheat, water bath sonicator extracts 4h at 35 DEG C.
(5) the buckwheat slurry 4000r/min centrifugation 15min after water bath sonicator is extracted, collect supernatant a and precipitation respectively
b。
(6) supernatant a vacuum distillations are concentrated, is freeze-dried into powder, obtains flavones.
(7) add the deionized waters of 20 times of weight into precipitation b, with 0.1mol/L NaOH solution adjust after PH to 9
Water-bath ultrasonic extraction 2h obtains no flavones buckwheat precipitated liquid in 55 DEG C.
(8) 15min is centrifuged without flavones buckwheat precipitated liquid 4000r/min after water bath sonicator is extracted, is collected respectively
Clear liquid c and precipitation d；
(9) supernatant c vacuum distillations are concentrated, is freeze-dried into powder, obtains buck wheat protein；
(10) NaOH solution cyclic washings 4 time of the d with 1.0mol/L hydrochloric acid and 0.5mol/L will be precipitated, is then washed to
Neutrality, is freeze-dried into after powder and obtains buckwheat starch.
Embodiment 10
A kind of buckwheat starch extracting method：
(1) the buckwheat 500kg cleaned out is weighed, 6h is soaked with the water of 3 times of weight.
(2) soaked buckwheat is fallen into soaking water, cleaning adds the weight such as the buckwheat that is weighed with step (1) after draining
Water, grind to form slurry.It is preferred that can use Buddha's warrior attendant be sanded be ground.
(3) sub-sieve for being 0.149mm with sieve aperture filters out wheat bran, filters out fine fibre for 0.088 sub-sieve with sieve aperture, obtains
To buckwheat slurry.
(4) 80% ethanol solution of 20 times of weight is added in being starched to buckwheat, water bath sonicator extracts 2.5h at 35 DEG C.
(5) the buckwheat slurry 4000r/min centrifugation 15min after water bath sonicator is extracted, collect supernatant a and precipitation respectively
b。
(6) supernatant a vacuum distillations are concentrated, is freeze-dried into powder, obtains flavones.
(7) add the deionized waters of 15 times of weight into precipitation b, with 0.1mol/L NaOH solution adjust after PH to 9
Water-bath ultrasonic extraction 2h obtains no flavones buckwheat precipitated liquid in 55 DEG C.
(8) 15min is centrifuged without flavones buckwheat precipitated liquid 4000r/min after water bath sonicator is extracted, is collected respectively
Clear liquid c and precipitation d；
(9) supernatant c vacuum distillations are concentrated, is freeze-dried into powder, obtains buck wheat protein；
(10) NaOH solution cyclic washings 4 time of the d with 1.0mol/L hydrochloric acid and 0.5mol/L will be precipitated, is then washed to
Neutrality, is freeze-dried into after powder and obtains buckwheat starch.
Embodiment 11
A kind of buckwheat starch extracting method：
(1) the buckwheat 500kg cleaned out is weighed, 6h is soaked with the water of 3 times of weight.
(2) soaked buckwheat is fallen into soaking water, cleaning adds the weight such as the buckwheat that is weighed with step (1) after draining
Water, grind to form slurry.It is preferred that can use Buddha's warrior attendant be sanded be ground.
(3) sub-sieve for being 0.149mm with sieve aperture filters out wheat bran, filters out fine fibre for 0.088 sub-sieve with sieve aperture, obtains
To buckwheat slurry.
(4) 80% ethanol solution of 20 times of weight is added in being starched to buckwheat, water bath sonicator extracts 2.5h at 35 DEG C.
(5) the buckwheat slurry 4000r/min centrifugation 15min after water bath sonicator is extracted, collect supernatant a and precipitation respectively
b。
(6) supernatant a vacuum distillations are concentrated, is freeze-dried into powder, obtains flavones.
(7) add the deionized waters of 25 times of weight into precipitation b, with 0.1mol/L NaOH solution adjust after PH to 9
Water-bath ultrasonic extraction 2h obtains no flavones buckwheat precipitated liquid in 55 DEG C.
(8) 15min is centrifuged without flavones buckwheat precipitated liquid 4000r/min after water bath sonicator is extracted, is collected respectively
Clear liquid c and precipitation d；
(9) supernatant c vacuum distillations are concentrated, is freeze-dried into powder, obtains buck wheat protein；
(10) NaOH solution cyclic washings 4 time of the d with 1.0mol/L hydrochloric acid and 0.5mol/L will be precipitated, is then washed to
Neutrality, is freeze-dried into after powder and obtains buckwheat starch.
Embodiment 12
A kind of buckwheat starch extracting method：
(1) the buckwheat 500kg cleaned out is weighed, 6h is soaked with the water of 3 times of weight.
(2) soaked buckwheat is fallen into soaking water, cleaning adds the weight such as the buckwheat that is weighed with step (1) after draining
Water, grind to form slurry.It is preferred that can use Buddha's warrior attendant be sanded be ground.
(3) sub-sieve for being 0.149mm with sieve aperture filters out wheat bran, filters out fine fibre for 0.088 sub-sieve with sieve aperture, obtains
To buckwheat slurry.
(4) 80% ethanol solution of 20 times of weight is added in being starched to buckwheat, water bath sonicator extracts 2.5h at 35 DEG C.
(5) the buckwheat slurry 4000r/min centrifugation 15min after water bath sonicator is extracted, collect supernatant a and precipitation respectively
b。
(6) supernatant a vacuum distillations are concentrated, is freeze-dried into powder, obtains flavones.
(7) add the deionized waters of 20 times of weight into precipitation b, with 0.1mol/L NaOH solution adjust after PH to 8
Water-bath ultrasonic extraction 2h obtains no flavones buckwheat precipitated liquid in 55 DEG C.
(8) 15min is centrifuged without flavones buckwheat precipitated liquid 4000r/min after water bath sonicator is extracted, is collected respectively
Clear liquid c and precipitation d；
(9) supernatant c vacuum distillations are concentrated, is freeze-dried into powder, obtains buck wheat protein；
(10) NaOH solution cyclic washings 4 time of the d with 1.0mol/L hydrochloric acid and 0.5mol/L will be precipitated, is then washed to
Neutrality, is freeze-dried into after powder and obtains buckwheat starch.
Embodiment 13
A kind of buckwheat starch extracting method：
(1) the buckwheat 500kg cleaned out is weighed, 6h is soaked with the water of 3 times of weight.
(2) soaked buckwheat is fallen into soaking water, cleaning adds the weight such as the buckwheat that is weighed with step (1) after draining
Water, grind to form slurry.It is preferred that can use Buddha's warrior attendant be sanded be ground.
(3) sub-sieve for being 0.149mm with sieve aperture filters out wheat bran, filters out fine fibre for 0.088 sub-sieve with sieve aperture, obtains
To buckwheat slurry.
(4) 80% ethanol solution of 20 times of weight is added in being starched to buckwheat, water bath sonicator extracts 2.5h at 35 DEG C.
(5) the buckwheat slurry 4000r/min centrifugation 15min after water bath sonicator is extracted, collect supernatant a and precipitation respectively
b。
(6) supernatant a vacuum distillations are concentrated, is freeze-dried into powder, obtains flavones.
(7) add the deionized waters of 20 times of weight into precipitation b, with 0.1mol/L NaOH solution adjust after PH to 10
Water-bath ultrasonic extraction 2h obtains no flavones buckwheat precipitated liquid in 55 DEG C.
(8) 15min is centrifuged without flavones buckwheat precipitated liquid 4000r/min after water bath sonicator is extracted, is collected respectively
Clear liquid c and precipitation d；
(9) supernatant c vacuum distillations are concentrated, is freeze-dried into powder, obtains buck wheat protein；
(10) NaOH solution cyclic washings 4 time of the d with 1.0mol/L hydrochloric acid and 0.5mol/L will be precipitated, is then washed to
Neutrality, is freeze-dried into after powder and obtains buckwheat starch.
Embodiment 14
A kind of buckwheat starch extracting method：
(1) the buckwheat 500kg cleaned out is weighed, 6h is soaked with the water of 3 times of weight.
(2) soaked buckwheat is fallen into soaking water, cleaning adds the weight such as the buckwheat that is weighed with step (1) after draining
Water, grind to form slurry.It is preferred that can use Buddha's warrior attendant be sanded be ground.
(3) sub-sieve for being 0.149mm with sieve aperture filters out wheat bran, filters out fine fibre for 0.088 sub-sieve with sieve aperture, obtains
To buckwheat slurry.
(4) 80% ethanol solution of 20 times of weight is added in being starched to buckwheat, water bath sonicator extracts 2.5h at 35 DEG C.
(5) the buckwheat slurry 4000r/min centrifugation 15min after water bath sonicator is extracted, collect supernatant a and precipitation respectively
b。
(6) supernatant a vacuum distillations are concentrated, is freeze-dried into powder, obtains flavones.
(7) add the deionized waters of 20 times of weight into precipitation b, with 0.1mol/L NaOH solution adjust after PH to 9
Water-bath ultrasonic extraction 2h obtains no flavones buckwheat precipitated liquid in 45 DEG C.
(8) 15min is centrifuged without flavones buckwheat precipitated liquid 4000r/min after water bath sonicator is extracted, is collected respectively
Clear liquid c and precipitation d；
(9) supernatant c vacuum distillations are concentrated, is freeze-dried into powder, obtains buck wheat protein；
(10) NaOH solution cyclic washings 4 time of the d with 1.0mol/L hydrochloric acid and 0.5mol/L will be precipitated, is then washed to
Neutrality, is freeze-dried into after powder and obtains buckwheat starch.
Embodiment 15
A kind of buckwheat starch extracting method：
(1) the buckwheat 500kg cleaned out is weighed, 6h is soaked with the water of 3 times of weight.
(2) soaked buckwheat is fallen into soaking water, cleaning adds the weight such as the buckwheat that is weighed with step (1) after draining
Water, grind to form slurry.It is preferred that can use Buddha's warrior attendant be sanded be ground.
(3) sub-sieve for being 0.149mm with sieve aperture filters out wheat bran, filters out fine fibre for 0.088 sub-sieve with sieve aperture, obtains
To buckwheat slurry.
(4) 80% ethanol solution of 20 times of weight is added in being starched to buckwheat, water bath sonicator extracts 2.5h at 35 DEG C.
(5) the buckwheat slurry 4000r/min centrifugation 15min after water bath sonicator is extracted, collect supernatant a and precipitation respectively
b。
(6) supernatant a vacuum distillations are concentrated, is freeze-dried into powder, obtains flavones.
(7) add the deionized waters of 20 times of weight into precipitation b, with 0.1mol/L NaOH solution adjust after PH to 9
Water-bath ultrasonic extraction 2h obtains no flavones buckwheat precipitated liquid in 60 DEG C.
(8) 15min is centrifuged without flavones buckwheat precipitated liquid 4000r/min after water bath sonicator is extracted, is collected respectively
Clear liquid c and precipitation d；
(9) supernatant c vacuum distillations are concentrated, is freeze-dried into powder, obtains buck wheat protein；
(10) NaOH solution cyclic washings 4 time of the d with 1.0mol/L hydrochloric acid and 0.5mol/L will be precipitated, is then washed to
Neutrality, is freeze-dried into after powder and obtains buckwheat starch.
Embodiment 16
A kind of buckwheat starch extracting method：
(1) the buckwheat 500kg cleaned out is weighed, 6h is soaked with the water of 3 times of weight.
(2) soaked buckwheat is fallen into soaking water, cleaning adds the weight such as the buckwheat that is weighed with step (1) after draining
Water, grind to form slurry.It is preferred that can use Buddha's warrior attendant be sanded be ground.
(3) sub-sieve for being 0.149mm with sieve aperture filters out wheat bran, filters out fine fibre for 0.088 sub-sieve with sieve aperture, obtains
To buckwheat slurry.
(4) 80% ethanol solution of 20 times of weight is added in being starched to buckwheat, water bath sonicator extracts 2.5h at 35 DEG C.
(5) the buckwheat slurry 4000r/min centrifugation 15min after water bath sonicator is extracted, collect supernatant a and precipitation respectively
b。
(6) supernatant a vacuum distillations are concentrated, is freeze-dried into powder, obtains flavones.
(7) add the deionized waters of 20 times of weight into precipitation b, with 0.1mol/L NaOH solution adjust after PH to 9
Water-bath ultrasonic extraction 1h obtains no flavones buckwheat precipitated liquid in 55 DEG C.
(8) 15min is centrifuged without flavones buckwheat precipitated liquid 4000r/min after water bath sonicator is extracted, is collected respectively
Clear liquid c and precipitation d；
(9) supernatant c vacuum distillations are concentrated, is freeze-dried into powder, obtains buck wheat protein；
(10) NaOH solution cyclic washings 4 time of the d with 1.0mol/L hydrochloric acid and 0.5mol/L will be precipitated, is then washed to
Neutrality, is freeze-dried into after powder and obtains buckwheat starch.
Embodiment 17
A kind of buckwheat starch extracting method：
(1) the buckwheat 500kg cleaned out is weighed, 6h is soaked with the water of 3 times of weight.
(2) soaked buckwheat is fallen into soaking water, cleaning adds the weight such as the buckwheat that is weighed with step (1) after draining
Water, grind to form slurry.It is preferred that can use Buddha's warrior attendant be sanded be ground.
(3) sub-sieve for being 0.149mm with sieve aperture filters out wheat bran, filters out fine fibre for 0.088 sub-sieve with sieve aperture, obtains
To buckwheat slurry.
(4) 80% ethanol solution of 20 times of weight is added in being starched to buckwheat, water bath sonicator extracts 2.5h at 35 DEG C.
(5) the buckwheat slurry 4000r/min centrifugation 15min after water bath sonicator is extracted, collect supernatant a and precipitation respectively
b。
(6) supernatant a vacuum distillations are concentrated, is freeze-dried into powder, obtains flavones.
(7) add the deionized waters of 20 times of weight into precipitation b, with 0.1mol/L NaOH solution adjust after PH to 9
Water-bath ultrasonic extraction 5h obtains no flavones buckwheat precipitated liquid in 55 DEG C.
(8) 15min is centrifuged without flavones buckwheat precipitated liquid 4000r/min after water bath sonicator is extracted, is collected respectively
Clear liquid c and precipitation d；
(9) supernatant c vacuum distillations are concentrated, is freeze-dried into powder, obtains buck wheat protein；
(10) NaOH solution cyclic washings 4 time of the d with 1.0mol/L hydrochloric acid and 0.5mol/L will be precipitated, is then washed to
Neutrality, is freeze-dried into after powder and obtains buckwheat starch.
Embodiment 1-9 is contrasted：
Contrast understands that Extracting temperature influence is smaller during separation and Extraction flavones, when concentration of alcohol, solid-liquid ratio, extraction
Between influence larger.
Embodiment 1, embodiment 10-17 are contrasted：
Contrast understands that extraction time influences smaller, solution pH value, solid-liquid ratio, extraction in separation and Extraction protein processes
Temperature influence is larger.Other may be caused to have under the recovery rate of protein, basic conditions although improving solution PH and can increase
Color substance dissolution, influences lipidated protein.
Finally it is pointed out that above example is only the more representational example of the present invention.Obviously, technology of the invention
Scheme is not limited to above-described embodiment, can also there is many deformations.One of ordinary skill in the art can be from disclosed by the invention
All deformations that content is directly exported or associated, are considered as protection scope of the present invention.
Claims (7)
1. a kind of buckwheat starch extracting method, comprises the following steps：
(1) buckwheat cleaned out is weighed, 4-8h is soaked with 2-3 times of water；
(2) soaked buckwheat is fallen into soaking water, cleaning adds the weight such as the buckwheat that is weighed with step (1) after draining
Water, grinds to form slurry；
(3) sub-sieve for being 0.149mm with sieve aperture filters out wheat bran, filters out fine fibre for 0.088 sub-sieve with sieve aperture, obtains buckwheat
Wheat is starched；
(4) the 70%-90% ethanol solutions of 15-25 times of weight are added in being starched to buckwheat, water bath sonicator is extracted at 25 DEG C -50 DEG C
2-4h；
(5) the buckwheat slurry 4000r/min centrifugation 15min after water bath sonicator is extracted, collect supernatant a and precipitation b respectively；
(6) supernatant a concentrations are evaporated, obtain flavones；
(7) deionized water of 15-25 times of weight is added into precipitation b, is adjusted with 0.1mol/L NaOH solution after PH to 8-10
The water-bath ultrasonic extraction 1-5h in 45 DEG C -60 DEG C, obtains no flavones buckwheat precipitated liquid；
(8) 15min is centrifuged without flavones buckwheat precipitated liquid 4000r/min after water bath sonicator is extracted, supernatant c is collected respectively
And precipitation d；
(9) supernatant c concentrations are evaporated, obtain buck wheat protein；
(10) NaOH solution cyclic washings 3-5 time of the d with 1.0mol/L hydrochloric acid and 0.5mol/L will be precipitated, in being then washed to
Property, obtain buckwheat starch after dehydrating.
2. buckwheat starch extracting method as claimed in claim 1, it is characterised in that：The slurry that grinds to form in step (2) is to use
Buddha's warrior attendant is sanded buckwheat grinding to form slurry.
3. buckwheat starch extracting method as claimed in claim 1, it is characterised in that step (4) is：20 are added in being starched to buckwheat
80% ethanol solution of times of weight, water bath sonicator extracts 2.5h at 35 DEG C.
4. buckwheat starch extracting method as claimed in claim 1, it is characterised in that it is to use to subtract that the concentration in step (6), which is evaporated,
Supernatant a concentrations are evaporated by the method that pressure distillation and freeze-drying are combined.
5. buckwheat starch extracting method as claimed in claim 1, it is characterised in that step (7) is：20 weights are added into precipitation b
The deionized water of amount times, the water-bath ultrasonic extraction 2h in 55 DEG C is adjusted after PH to 9 with 0.1mol/L NaOH solution.
6. buckwheat starch extracting method as claimed in claim 1, it is characterised in that concentrating supernatant c in step (9) is steamed
Dry is that supernatant c concentrations are evaporated by the method being combined using vacuum distillation and freeze-drying.
7. buckwheat starch extracting method as claimed in claim 1, it is characterised in that dehydrating in step (10) is to use
The method of freeze-drying is dehydrated d is precipitated.
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| CN108124978A (en) * | 2018-03-15 | 2018-06-08 | 成都大学 | A kind of processing method of buckwheat odor type dried bean curd |
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| CN108124978A (en) * | 2018-03-15 | 2018-06-08 | 成都大学 | A kind of processing method of buckwheat odor type dried bean curd |
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Application publication date: 20170908 |