CN107130013A - A kind of engineering bacteriophage quick detection microorganism of aminopeptidase mark - Google Patents
A kind of engineering bacteriophage quick detection microorganism of aminopeptidase mark Download PDFInfo
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- CN107130013A CN107130013A CN201710339620.1A CN201710339620A CN107130013A CN 107130013 A CN107130013 A CN 107130013A CN 201710339620 A CN201710339620 A CN 201710339620A CN 107130013 A CN107130013 A CN 107130013A
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Abstract
This work is intended have developed microorganism in the engineering bacteriophage kit Direct Analysis environmental and biological materialses that an aminopeptidase is marked, and the engineering bacteriophage of this aminopeptidase mark can be with specific recognition pathogenic microorganism.After bacteriophage identification combination microorganism is engineered, the DNA that its genetic engineering is transformed is injected in microbial body, the substantial amounts of bacteriophage with aminopeptidase albumen newly of recombination expression amplification in microbial body.Project main contents are：Key design is engineered bacteriophage and microorganism responsive materials recognition mechanism and kinetics, investigates these and recognizes the functional module responded action rule in microbial rapid detection and the instant expression analysis of microorganism.This work innovation is embodied in：Functionalization bacteriophage and microbial action relation are disclosed in basic research level, reference and reference are provided to solve to be related in microorganism detection method " online ", the electromechanical engineering of " portable " and " full-automatic " and biology Complex Problem.
Description
Technical field
A kind of engineering bacteriophage quick detection microorganism of aminopeptidase mark of present invention design.
Background technology
In marine environment, microbiological contamination, body eutrophication, microbiologic(al) corrosion, microorganism, which are stained, etc. is all
The apparent form of microbial threats human being's production life, is also the objective condition of rapid microbial detection technical need.Existing number
According to showing, the loss of microbiological contamination and microbial identification species speed are closely related, and the identification determination time is longer, loss
Also it is bigger.Because, one side microorganism growth and the speed propagated are fast, aggravate environmental pollution and human diseases；Separately
On the one hand microbe species can not be specified to lead to not implement specific aim protectiving scheme, and then cause some drugses or antiseptic
Abuse.In ISO4883-2003 standards, the microbial identification time is identified as the important of microorganism detection industrial grade division
Parameter.Therefore, taking effective method quick detection microorganism microorganism disease and is stained effective in reduction marine environment
Means.
Microorganism detection and the theory of identification are extensively dependent on the response of microorganism feature product（Such as ATP responses luciferase,
Aminopeptidase response chloro- 3- indoles-β-D galactosides of the bromo- 4- of 5- and H2S responses Fe2+ of E. coli secretion etc.）, micro- life
(such as antibody-microbial cell, agglutinin-antimicrobial surface glycogen, antibiotic-the micro- life of thing cell surface antigen specific recognition
Thing surface specific site and aptamers-antimicrobial surface recognition site etc.) and microbial gene analysis（DNA and 16s RNA）.
For feature product response detection microorganism, it determines the microbe quantity in water sample, and its specificity is very weak.By in water sample
Middle addition Basionic, discharges ATP in microbial cell, adds luciferase, and this enzyme can be with ATP
Effect causes photochemical reaction, produces quantitative light intensity.To antimicrobial surface antigentic specificity identification for, its using immune response as
Basis recognizes and detected microorganism, and the data that this method is obtained are accurate, but can not as a kind of detection instrument of online live body,
Easily influenceed by extraneous factor.Our seminars have carried out a series of work in terms of microorganism detection：Explore based on enzyme-linked
It is micro- that the electro-chemistry immunity biology sensor that the no signal mark and nano material signal of immune response are marked comes quick detection ocean
It is biological.The expansion and comparative study of the two aspect work, new approaches are provided for the development of microorganism detection technology.To microorganism base
For analytical technology, it is widely used in microbial identification, and target spot primer used is the usual 16S according to microorganism
RRNA gene expression characteristicses sequence and design, its test limit can reach 1 cfu mL-1, and weak point is to need special sample
Processing and purifying, complex operation step have higher technical requirements.Weak point of this project from mentioned microorganism detection technique
Set out, research and develop portable microorganism care diagnostic instrument.
LAP is that content is very abundant in a kind of protease, liver.When liver inner-outer tube becomes silted up, LAP vigor significantly increases, and is especially disliking
Property courage become silted up when, its vigor persistently increases with disease progression.Reagent blocks liver road and the diagnosis of cancer of pancreas is valuable.Liver is bad
Subcutaneous ulcer, liver tumour, hepatitis, breast cancer, liver cancer, cancer of bile ducts, cancer of pancreas, carcinoma of endometrium, oophoroma substantially increase.Hepatic sclerosis, biography
Metachromia hepatitis can moderate increase, be often 2-4 times of reference value.Obstructive jaundice substantially increases, and often reaches reference value more than 5 times, and
Appear in before bilirubin or ALP rise.Unlike the enzyme of other liver functions, blood sample can only be detected, LAP can also detect urine
Liquid.The change of LAP in urine can be detected in some cases, without taking a blood sample.Leucine aminopeptidase（LAP）LAP is in people
Body is widely present in respectively organizing, when toxicant or sickness influence are to the proximal tubule for being rich in LAP, the active highests of urine LAP.Kidney
Bead substrate membrane permeability increases, renal cells is damaged, medicine causes LAP when nephrotoxicity and kidney neoplasms to increase.
LAP is urinated after oncotherapy and increases prompting tumor recurrence.Various kidney cases are analyzed, LAP positive rate highests are found.
Microorganism detection technology and imperfections, in addition it is also necessary to sustainable development and innovation.Microorganism detection field innovative point may
Based in terms of following four：One is to utilize the application of micro-nano Novel electronic devices and photoelectric device in microorganism detection.Two are
Multifunction device integrated system is separated to microorganism, screened and tested and analyzed.Three be the analysis system combination of Multi-example
Multimode detecting system is analyzed microorganism or synchronized to multichannel data acquisition system.Four be based on smart mobile phone just
Formula real-time test (point-of-care testing, POCT) technology is taken, makes the portable device and mobile Internet of personalization
With reference to.These are all the directions of innovation and development microorganism detection research.This work is reflected using biomolecule functionalization micron openings
Fixed and analysis microorganism, its signal detected, which mostlys come from biomolecule and target spot microorganism detection and can extend microorganism, to be passed through
The time of micron openings, and then obtain related microbial standard curve.
The content of the invention
To achieve the above object, the present invention use technical scheme for：
A kind of engineering bacteriophage quick detection microorganism of aminopeptidase mark, it is characterised in that：Including specific phagocytosis
The aminopeptidase gene of body, functionalization.
Such as the bacteriophage of specificity microorganism in right 1, specificity microorganism includes bacteriophage, the gold for being directed to Escherichia coli
The bacteriophage of staphylococcus aureus, the bacteriophage of salmonella, the bacteriophage of vibrios, the bacteriophage of Enterobacter sakazakii, small intestine knot
The bacteriophage of enteritis Yersinia ruckeri, the bacteriophage of C.perfringens, the bacteriophage of clostridium botulinum, the bacteriophage of anaerobic bacteria,
The bacteriophage of bacillus cereus.
Such as the bacteriophage of aminopeptidase functionalization in right 2：Its feature includes：T4 bacteriophages, T7 bacteriophages, P2 phagocytosis
Body, P22 bacteriophages, bacteriophage lambda, the bacteriophages of φ 29.
Such as the bacteriophage of aminopeptidase functionalization in right 3, its feature is included in gp10B GFP terminal fusion amino
Peptidase genes.
Brief description of the drawings
Fig. 1:The bacteriophage of aminopeptidase functionalization
（1）It is engineered bacteriophage；（2）Head；（3）It is coated gp-10B albumen；（4）Interior nucleoprotein；（5）Gp16 albumen；（6）gp15
Albumen；（7）Gp14 albumen；（8）Connector gp8；（9）Gp10B- aminopeptidase fusion proteins；（10）Gp6 and gp7 albumen；（11）
Gp11 and gp12 albumen；（12）Tail protein line gp17；（13）Afterbody；（14）For specified microorganisms host's associated proteins（Greatly
Enterobacteria, staphylococcus aureus, salmonella etc.）.
Fig. 2：Microbiological analysis system based on aminopeptidase functionalization bacteriophage.
Fig. 3：Sulfate with different reducing bacteria, Escherichia coli, staphylococcus aureus, the detection number of salmonella detection
Value.
Embodiment
Below by embodiment, the present invention will be further described.
Embodiment 1：
The detection of sulfate reducing bacteria：
The microorganism used in experiment utilizes bacteriolyze meat soup（Peptone 1%, sodium chloride 1%, yeast extract 0.5%, the mL of water 100）Suspend
Culture, single bacterium colony is centrifuged ten minutes in 4500 revs/min after incubated overnight under the conditions of 30 DEG C, 200 turns of shaking tables, and is buffered with PBS
Solution is diluted to various concentrations.
By 100 μ L concentration（10 cfu ml-1）The bacteriophage of bacterium solution and aminopeptidase mark is added to batch cultur
Base, and reacted 8 hours in 37 °C.Add the Valine 7- amino -4- methylcoumarin trifluoros of aminopeptidase response
Acetate hydrochloride, is cultivated 30 minutes, with the excitation source actuated sensor platform of microbe diagnosis instrument, produces light
Signal, measurement obtains the microbial signals under the concentration.
By 100 μ L concentration（102 cfu ml-1）The bacteriophage of bacterium solution and aminopeptidase mark is added to batch cultur
Base, and reacted 8 hours in 37 °C.Add the Valine 7- amino -4- methylcoumarin trifluoros of aminopeptidase response
Acetate hydrochloride, is cultivated 30 minutes, with the excitation source actuated sensor platform of microbe diagnosis instrument, produces light
Signal, measurement obtains the microbial signals under the concentration.
By 100 μ L concentration（103 cfu ml-1）The bacteriophage of bacterium solution and aminopeptidase mark is added to batch cultur
Base, and reacted 8 hours in 37 °C.Add the Valine 7- amino -4- methylcoumarin trifluoros of aminopeptidase response
Acetate hydrochloride, is cultivated 30 minutes, with the excitation source actuated sensor platform of microbe diagnosis instrument, produces light
Signal, measurement obtains the microbial signals under the concentration.
By 100 μ L concentration（104 cfu ml-1）The bacteriophage of bacterium solution and aminopeptidase mark is added to batch cultur
Base, and reacted 8 hours in 37 °C.Add the Valine 7- amino -4- methylcoumarin trifluoros of aminopeptidase response
Acetate hydrochloride, is cultivated 30 minutes, with the excitation source actuated sensor platform of microbe diagnosis instrument, produces light
Signal, measurement obtains the microbial signals under the concentration.
By 100 μ L concentration（105 cfu ml-1）The bacteriophage of bacterium solution and aminopeptidase mark is added to batch cultur
Base, and reacted 8 hours in 37 °C.Add the Valine 7- amino -4- methylcoumarin trifluoros of aminopeptidase response
Acetate hydrochloride, is cultivated 30 minutes, with the excitation source actuated sensor platform of microbe diagnosis instrument, produces light
Signal, measurement obtains the microbial signals under the concentration.
By 100 μ L concentration（106 cfu ml-1）The bacteriophage of bacterium solution and aminopeptidase mark is added to batch cultur
Base, and reacted 8 hours in 37 °C.Add the Valine 7- amino -4- methylcoumarin trifluoros of aminopeptidase response
Acetate hydrochloride, is cultivated 30 minutes, with the excitation source actuated sensor platform of microbe diagnosis instrument, produces light
Signal, measurement obtains the microbial signals under the concentration.
By 100 μ L concentration（107 cfu ml-1）The bacteriophage of bacterium solution and aminopeptidase mark is added to batch cultur
Base, and reacted 8 hours in 37 °C.Add the Valine 7- amino -4- methylcoumarin trifluoros of aminopeptidase response
Acetate hydrochloride, is cultivated 30 minutes, with the excitation source actuated sensor platform of microbe diagnosis instrument, produces light
Signal, measurement obtains the microbial signals under the concentration.
By 100 μ L concentration（108 cfu ml-1）The bacteriophage of bacterium solution and aminopeptidase mark is added to batch cultur
Base, and reacted 8 hours in 37 °C.Add the Valine 7- amino -4- methylcoumarin trifluoros of aminopeptidase response
Acetate hydrochloride, is cultivated 30 minutes, with the excitation source actuated sensor platform of microbe diagnosis instrument, produces light
Signal, measurement obtains the microbial signals under the concentration.
The data of summary, draw the standard curve of microorganism under various concentrations.
Embodiment 2：
E. coli detection
The microorganism used in experiment utilizes bacteriolyze meat soup（Peptone 1%, sodium chloride 1%, yeast extract 0.5%, the mL of water 100）Suspend
Culture, single bacterium colony is centrifuged ten minutes in 4500 revs/min after incubated overnight under the conditions of 30 DEG C, 200 turns of shaking tables, and is buffered with PBS
Solution is diluted to various concentrations.
By 100 μ L concentration（10 cfu ml-1）The bacteriophage of bacterium solution and aminopeptidase mark is added to batch cultur
Base, and reacted 8 hours in 37 °C.Add the Valine 7- amino -4- methylcoumarin trifluoros of aminopeptidase response
Acetate hydrochloride, is cultivated 30 minutes, with the excitation source actuated sensor platform of microbe diagnosis instrument, produces light
Signal, measurement obtains the microbial signals under the concentration.
By 100 μ L concentration（102 cfu ml-1）The bacteriophage of bacterium solution and aminopeptidase mark is added to batch cultur
Base, and reacted 8 hours in 37 °C.Add the Valine 7- amino -4- methylcoumarin trifluoros of aminopeptidase response
Acetate hydrochloride, is cultivated 30 minutes, with the excitation source actuated sensor platform of microbe diagnosis instrument, produces light
Signal, measurement obtains the microbial signals under the concentration.
By 100 μ L concentration（103 cfu ml-1）The bacteriophage of bacterium solution and aminopeptidase mark is added to batch cultur
Base, and reacted 8 hours in 37 °C.Add the Valine 7- amino -4- methylcoumarin trifluoros of aminopeptidase response
Acetate hydrochloride, is cultivated 30 minutes, with the excitation source actuated sensor platform of microbe diagnosis instrument, produces light
Signal, measurement obtains the microbial signals under the concentration.
By 100 μ L concentration（104 cfu ml-1）The bacteriophage of bacterium solution and aminopeptidase mark is added to batch cultur
Base, and reacted 8 hours in 37 °C.Add the Valine 7- amino -4- methylcoumarin trifluoros of aminopeptidase response
Acetate hydrochloride, is cultivated 30 minutes, with the excitation source actuated sensor platform of microbe diagnosis instrument, produces light
Signal, measurement obtains the microbial signals under the concentration.
By 100 μ L concentration（105 cfu ml-1）The bacteriophage of bacterium solution and aminopeptidase mark is added to batch cultur
Base, and reacted 8 hours in 37 °C.Add the Valine 7- amino -4- methylcoumarin trifluoros of aminopeptidase response
Acetate hydrochloride, is cultivated 30 minutes, with the excitation source actuated sensor platform of microbe diagnosis instrument, produces light
Signal, measurement obtains the microbial signals under the concentration.
By 100 μ L concentration（106 cfu ml-1）The bacteriophage of bacterium solution and aminopeptidase mark is added to batch cultur
Base, and reacted 8 hours in 37 °C.Add the Valine 7- amino -4- methylcoumarin trifluoros of aminopeptidase response
Acetate hydrochloride, is cultivated 30 minutes, with the excitation source actuated sensor platform of microbe diagnosis instrument, produces light
Signal, measurement obtains the microbial signals under the concentration.
By 100 μ L concentration（107 cfu ml-1）The bacteriophage of bacterium solution and aminopeptidase mark is added to batch cultur
Base, and reacted 8 hours in 37 °C.Add the Valine 7- amino -4- methylcoumarin trifluoros of aminopeptidase response
Acetate hydrochloride, is cultivated 30 minutes, with the excitation source actuated sensor platform of microbe diagnosis instrument, produces light
Signal, measurement obtains the microbial signals under the concentration.
By 100 μ L concentration（108 cfu ml-1）The bacteriophage of bacterium solution and aminopeptidase mark is added to batch cultur
Base, and reacted 8 hours in 37 °C.Add the Valine 7- amino -4- methylcoumarin trifluoros of aminopeptidase response
Acetate hydrochloride, is cultivated 30 minutes, with the excitation source actuated sensor platform of microbe diagnosis instrument, produces light
Signal, measurement obtains the microbial signals under the concentration.
The data of summary, draw the standard curve of microorganism under various concentrations.
Embodiment 3：
Staphylococcus aureus detects
The microorganism used in experiment utilizes bacteriolyze meat soup（Peptone 1%, sodium chloride 1%, yeast extract 0.5%, the mL of water 100）Suspend
Culture, single bacterium colony is centrifuged ten minutes in 4500 revs/min after incubated overnight under the conditions of 30 DEG C, 200 turns of shaking tables, and is buffered with PBS
Solution is diluted to various concentrations.
By 100 μ L concentration（10 cfu ml-1）The bacteriophage of bacterium solution and aminopeptidase mark is added to batch cultur
Base, and reacted 8 hours in 37 °C.Add the Valine 7- amino -4- methylcoumarin trifluoros of aminopeptidase response
Acetate hydrochloride, is cultivated 30 minutes, with the excitation source actuated sensor platform of microbe diagnosis instrument, produces light
Signal, measurement obtains the microbial signals under the concentration.
By 100 μ L concentration（102 cfu ml-1）The bacteriophage of bacterium solution and aminopeptidase mark is added to batch cultur
Base, and reacted 8 hours in 37 °C.Add the Valine 7- amino -4- methylcoumarin trifluoros of aminopeptidase response
Acetate hydrochloride, is cultivated 30 minutes, with the excitation source actuated sensor platform of microbe diagnosis instrument, produces light
Signal, measurement obtains the microbial signals under the concentration.
By 100 μ L concentration（103 cfu ml-1）The bacteriophage of bacterium solution and aminopeptidase mark is added to batch cultur
Base, and reacted 8 hours in 37 °C.Add the Valine 7- amino -4- methylcoumarin trifluoros of aminopeptidase response
Acetate hydrochloride, is cultivated 30 minutes, with the excitation source actuated sensor platform of microbe diagnosis instrument, produces light
Signal, measurement obtains the microbial signals under the concentration.
By 100 μ L concentration（104 cfu ml-1）The bacteriophage of bacterium solution and aminopeptidase mark is added to batch cultur
Base, and reacted 8 hours in 37 °C.Add the Valine 7- amino -4- methylcoumarin trifluoros of aminopeptidase response
Acetate hydrochloride, is cultivated 30 minutes, with the excitation source actuated sensor platform of microbe diagnosis instrument, produces light
Signal, measurement obtains the microbial signals under the concentration.
By 100 μ L concentration（105 cfu ml-1）The bacteriophage of bacterium solution and aminopeptidase mark is added to batch cultur
Base, and reacted 8 hours in 37 °C.Add the Valine 7- amino -4- methylcoumarin trifluoros of aminopeptidase response
Acetate hydrochloride, is cultivated 30 minutes, with the excitation source actuated sensor platform of microbe diagnosis instrument, produces light
Signal, measurement obtains the microbial signals under the concentration.
By 100 μ L concentration（106 cfu ml-1）The bacteriophage of bacterium solution and aminopeptidase mark is added to batch cultur
Base, and reacted 8 hours in 37 °C.Add the Valine 7- amino -4- methylcoumarin trifluoros of aminopeptidase response
Acetate hydrochloride, is cultivated 30 minutes, with the excitation source actuated sensor platform of microbe diagnosis instrument, produces light
Signal, measurement obtains the microbial signals under the concentration.
By 100 μ L concentration（107 cfu ml-1）The bacteriophage of bacterium solution and aminopeptidase mark is added to batch cultur
Base, and reacted 8 hours in 37 °C.Add the Valine 7- amino -4- methylcoumarin trifluoros of aminopeptidase response
Acetate hydrochloride, is cultivated 30 minutes, with the excitation source actuated sensor platform of microbe diagnosis instrument, produces light
Signal, measurement obtains the microbial signals under the concentration.
By 100 μ L concentration（108 cfu ml-1）The bacteriophage of bacterium solution and aminopeptidase mark is added to batch cultur
Base, and reacted 8 hours in 37 °C.Add the Valine 7- amino -4- methylcoumarin trifluoros of aminopeptidase response
Acetate hydrochloride, is cultivated 30 minutes, with the excitation source actuated sensor platform of microbe diagnosis instrument, produces light
Signal, measurement obtains the microbial signals under the concentration.
The data of summary, draw the standard curve of microorganism under various concentrations.
Embodiment 4：
Salmonella detection detection
The microorganism used in experiment utilizes bacteriolyze meat soup（Peptone 1%, sodium chloride 1%, yeast extract 0.5%, the mL of water 100）Suspend
Culture, single bacterium colony is centrifuged ten minutes in 4500 revs/min after incubated overnight under the conditions of 30 DEG C, 200 turns of shaking tables, and is buffered with PBS
Solution is diluted to various concentrations.
By 100 μ L concentration（10 cfu ml-1）The bacteriophage of bacterium solution and aminopeptidase mark is added to batch cultur
Base, and reacted 8 hours in 37 °C.Add the Valine 7- amino -4- methylcoumarin trifluoros of aminopeptidase response
Acetate hydrochloride, is cultivated 30 minutes, with the excitation source actuated sensor platform of microbe diagnosis instrument, produces light
Signal, measurement obtains the microbial signals under the concentration.
By 100 μ L concentration（102 cfu ml-1）The bacteriophage of bacterium solution and aminopeptidase mark is added to batch cultur
Base, and reacted 8 hours in 37 °C.Add the Valine 7- amino -4- methylcoumarin trifluoros of aminopeptidase response
Acetate hydrochloride, is cultivated 30 minutes, with the excitation source actuated sensor platform of microbe diagnosis instrument, produces light
Signal, measurement obtains the microbial signals under the concentration.
By 100 μ L concentration（103 cfu ml-1）The bacteriophage of bacterium solution and aminopeptidase mark is added to batch cultur
Base, and reacted 8 hours in 37 °C.Add the Valine 7- amino -4- methylcoumarin trifluoros of aminopeptidase response
Acetate hydrochloride, is cultivated 30 minutes, with the excitation source actuated sensor platform of microbe diagnosis instrument, produces light
Signal, measurement obtains the microbial signals under the concentration.
By 100 μ L concentration（104 cfu ml-1）The bacteriophage of bacterium solution and aminopeptidase mark is added to batch cultur
Base, and reacted 8 hours in 37 °C.Add the Valine 7- amino -4- methylcoumarin trifluoros of aminopeptidase response
Acetate hydrochloride, is cultivated 30 minutes, with the excitation source actuated sensor platform of microbe diagnosis instrument, produces light
Signal, measurement obtains the microbial signals under the concentration.
By 100 μ L concentration（105 cfu ml-1）The bacteriophage of bacterium solution and aminopeptidase mark is added to batch cultur
Base, and reacted 8 hours in 37 °C.Add the Valine 7- amino -4- methylcoumarin trifluoros of aminopeptidase response
Acetate hydrochloride, is cultivated 30 minutes, with the excitation source actuated sensor platform of microbe diagnosis instrument, produces light
Signal, measurement obtains the microbial signals under the concentration.
By 100 μ L concentration（106 cfu ml-1）The bacteriophage of bacterium solution and aminopeptidase mark is added to batch cultur
Base, and reacted 8 hours in 37 °C.Add the Valine 7- amino -4- methylcoumarin trifluoros of aminopeptidase response
Acetate hydrochloride, is cultivated 30 minutes, with the excitation source actuated sensor platform of microbe diagnosis instrument, produces light
Signal, measurement obtains the microbial signals under the concentration.
By 100 μ L concentration（107 cfu ml-1）The bacteriophage of bacterium solution and aminopeptidase mark is added to batch cultur
Base, and reacted 8 hours in 37 °C.Add the Valine 7- amino -4- methylcoumarin trifluoros of aminopeptidase response
Acetate hydrochloride, is cultivated 30 minutes, with the excitation source actuated sensor platform of microbe diagnosis instrument, produces light
Signal, measurement obtains the microbial signals under the concentration.
By 100 μ L concentration（108 cfu ml-1）The bacteriophage of bacterium solution and aminopeptidase mark is added to batch cultur
Base, and reacted 8 hours in 37 °C.Add the Valine 7- amino -4- methylcoumarin trifluoros of aminopeptidase response
Acetate hydrochloride, is cultivated 30 minutes, with the excitation source actuated sensor platform of microbe diagnosis instrument, produces light
Signal, measurement obtains the microbial signals under the concentration.
The data of summary, draw the standard curve of microorganism under various concentrations.
Claims (4)
1. a kind of engineering bacteriophage quick detection microorganism of aminopeptidase mark, it is characterised in that：Bitten including specific
The aminopeptidase gene of thalline, functionalization.
2. such as the bacteriophage of specificity microorganism in right 1, specificity microorganism includes bacteriophage for Escherichia coli, golden yellow
The staphylococcic bacteriophage of color, the bacteriophage of salmonella, the bacteriophage of vibrios, the bacteriophage of Enterobacter sakazakii, small intestine colon
The bacteriophage of scorching Yersinia ruckeri, the bacteriophage of C.perfringens, the bacteriophage of clostridium botulinum, the bacteriophage of anaerobic bacteria, wax
The bacteriophage of sample bacillus.
3. such as the bacteriophage of aminopeptidase functionalization in right 2：Its feature includes：T4 bacteriophages, T7 bacteriophages, P2 bacteriophages,
P22 bacteriophages, bacteriophage lambda, the bacteriophages of φ 29.
4. such as the bacteriophage of aminopeptidase functionalization in right 3, its feature is included in gp10B GFP terminal fusion amino peptides
Enzyme gene.
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