CN107125653A - The preparation method of pomelo peel Epigallo-catechin gallate (EGCG) compound - Google Patents
The preparation method of pomelo peel Epigallo-catechin gallate (EGCG) compound Download PDFInfo
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- CN107125653A CN107125653A CN201710325956.2A CN201710325956A CN107125653A CN 107125653 A CN107125653 A CN 107125653A CN 201710325956 A CN201710325956 A CN 201710325956A CN 107125653 A CN107125653 A CN 107125653A
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- pomelo peel
- egcg
- epigallo
- catechin gallate
- compound
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L19/00—Products from fruits or vegetables; Preparation or treatment thereof
- A23L19/03—Products from fruits or vegetables; Preparation or treatment thereof consisting of whole pieces or fragments without mashing the original pieces
- A23L19/07—Fruit waste products, e.g. from citrus peel or seeds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Mycology (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Pyrane Compounds (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention belongs to field of deep processing of farm products, a kind of preparation method of pomelo peel Epigallo-catechin gallate (EGCG) compound is specifically provided.Using cheap, wide material sources pomelo peels as raw material in described method, shaddock peel adsorbent is obtained by elution, drying process, then the pomelo peel after rinse and Epigallo-catechin gallate (EGCG) are acted on forming adsorption complex in solution absorption system by physical/chemical adsorption, compound after adsorption equilibrium is passed through into frozen drying, the pomelo peel Epigallo-catechin gallate (EGCG) adsorption complex of solid forms is obtained, the compound can improve the efficiency that Epigallo-catechin gallate (EGCG) is absorbed by the body；And the Epigallo-catechin gallate (EGCG) dietary fiber complex not decomposed by absorption can remove excessive free radicals in colonic lumen, reaching improves the effect that colon gives birth to environment, so as to improve the bioavailability of Epigallo-catechin gallate (EGCG).
Description
Technical field
The invention belongs to field of deep processing of farm products, a kind of pomelo peel-EGCG is specifically provided
The preparation method of ester complexes.
Background technology
Pomelo peel is the accessory substance of gardening product shaddock, is typically directly handled as food/agricultural wastes, neither
Environmental protection, wastes resource again, lacks the mode of the comprehensive utilization to pomelo peel.Epigallo-catechin gallate (EGCG) is a kind of
The catechins extracted in tealeaves, with various health care functions, but its intake human body after due to environmental pH and enzyme
The effect of class, its bioavailability is relatively low.At present, most of consumer can only be taken in by way of drinking tea.The present invention passes through
Series of process method, pomelo peel and Epigallo-catechin gallate (EGCG) are combined by suction-operated, form compound,
The purpose for the bioavailability for improving Epigallo-catechin gallate (EGCG) is reached, not yet there is pomelo peel and epi-nutgall at present
The preparation method report of catechin and gallate compound.
The content of the invention
It is an object of the invention to provide a kind of preparation side of pomelo peel-Epigallo-catechin gallate (EGCG) compound
Method.Additives are free of by preparing, is convenient for carrying and preserves, remain with original EGCG
The compound of ester healthcare function, improves pomelo peel comprehensive utilization value.
To achieve the above object, the present invention is adopted the following technical scheme that：
（1）Pomelo peel is dried to constant weight in 100-110 DEG C, 40 mesh sieves are crossed after crushing and obtain pomelo peel powder；
（2）Pomelo peel powder is positioned over and added in the Buchner funnel of No. 4 filter paper of Whatman, the NaOH for adding 0.1mol/L is molten
Liquid, carries out suction filtration, to filtrate pH stable, adds pure water and eluted；
（3）By step（2）Obtained pomelo peel powder, which is replaced in, to be added in the Buchner funnel of No. 4 filter paper of Whatman, is added
0.1mol/L HCl solution, carries out suction filtration, to filtrate pH stable, adds pure water and eluted；
（4）By step（3）Obtained pomelo peel powder, which is replaced in, to be added in the Buchner funnel of No. 4 filter paper of Whatman, is added
Ethanol solution, carries out suction filtration, adds pure water and eluted；
（5）By step（4）Obtained pomelo peel powder carries out frozen drying, obtains Solid Modified pomelo peel powder；
（6）Rinse solid pomelo peel powder, obtains the solid pomelo peel powder after rinse.By 10 g/L epi-nutgall catechu
Plain gallate is with the pomelo peel powder after rinse according to 1：4 ratio（v/w）Mixing, adsorption reaction is carried out at 25 DEG C.
（7）After 8 hours, it is up to the composite body of adsorption equilibrium and lies at -20 DEG C and be freeze-dried, obtains solid shape
The pomelo peel of state-Epigallo-catechin gallate (EGCG) compound.
Because the main component of pomelo peel is the lignocellulosic based on dietary fiber, and epigallocatechin does not have food
Sub- acid esters contains the functional groups such as hydroxyl, aromatic rings, can with the glucosides oxygen atom in dietary fiber component by Hydrogenbond,
It can be combined by covalent bonds such as ester bonds with polysaccharide, form chemisorbed；, can by transmission electron microscope and laser particle size analysis
It is made up of with seeing pomelo peel powder the little particle containing a large amount of micropores, these micropores can not have food as physical absorption table
The structure of sub- catechin and gallate.
Pomelo peel containing a large amount of dietary fibers is added in Epigallo-catechin gallate (EGCG) solution, passes through hydrogen
The physics chemical actions such as key, covalent bond or intermolecular attraction, form Epigallo-catechin gallate (EGCG)-dietary fiber multiple
Zoarium, in the presence of gastro-intestinal digestion liquid, these intermolecular weak forces can progressively be cleared up, and epigallocatechin is not had food
Sub- acid esters is slowly released from complex, and this process can increase Epigallo-catechin gallate (EGCG) in enteron aisle
RT, it is to avoid its too fast degraded under enteron aisle digestion, reach enhancing Epigallo-catechin gallate (EGCG)
The purpose being absorbed and utilized by the body；And the Epigallo-catechin gallate (EGCG) not being decomposed-dietary fiber complex is also
Colon can be entered, excessive free radicals in colonic lumen are removed, reaching improves the effect that colon gives birth to environment, so that improving table does not have food
The bioavailability of sub- catechin and gallate.
The remarkable advantage of the present invention is：
Additives are free of by preparing, is convenient for carrying and preserves, remain with original epigallocatechin nutgall
The compound of acid esters healthcare function, improves the bioavailability of Epigallo-catechin gallate (EGCG).
Brief description of the drawings
The FTIR spectrum figure of the Solid Modified pomelo peel powder of Fig. 1；
The laser particle size analysis of the Solid Modified pomelo peel powder of Fig. 2；
The SEM aspect graph of the Solid Modified pomelo peel powder of Fig. 3.
Embodiment：
For the further open rather than limitation present invention, below in conjunction with example, the present invention is described in further detail.
Embodiment 1
A kind of preparation method of pomelo peel-Epigallo-catechin gallate (EGCG) compound：
（1）Pomelo peel is dried to constant weight in 100-110 DEG C, 40 mesh sieves are crossed after crushing and obtain pomelo peel powder；
（2）Pomelo peel powder is positioned over and added in the Buchner funnel of No. 4 filter paper of Whatman, the NaOH for adding 0.1mol/L is molten
Liquid, carries out suction filtration, to filtrate pH stable, adds pure water and eluted；
（3）By step（2）Obtained pomelo peel powder, which is replaced in, to be added in the Buchner funnel of No. 4 filter paper of Whatman, is added
0.1mol/L HCl solution, carries out suction filtration, to filtrate pH stable, adds pure water and eluted；
（4）By step（3）Obtained pomelo peel powder, which is replaced in, to be added in the Buchner funnel of No. 4 filter paper of Whatman, is added
Ethanol solution, carries out suction filtration, adds pure water and eluted；
（5）By step（4）Obtained pomelo peel powder carries out frozen drying, obtains Solid Modified pomelo peel powder；
（6）Rinse solid pomelo peel powder, obtains the solid pomelo peel powder after rinse.By 10 g/L epi-nutgall catechu
Plain gallate is with the pomelo peel powder after rinse according to 1：4 ratio（v/w）Mixing, adsorption reaction is carried out at 25 DEG C.
（7）After 8 hours, it is up to the composite body of adsorption equilibrium and lies at -20 DEG C and be freeze-dried, obtains solid shape
The pomelo peel of state-Epigallo-catechin gallate (EGCG) compound.
The composition detection data of the Solid Modified pomelo peel of table 1
Table 1 illustrates：Content of material detection method in table, dietary fiber is pressed according to the methods describeds of GB/T 22224-2008, pectin
According to the methods describeds of NY/T 2016-2011, acidic cleaning lignin is pressed according to the methods describeds of GB/T 20805-2006, protein
Detected according to GB/T 5009.5-2010 methods describeds, the Solid Modified pomelo peel that detection data result is dried according to every 100g
In the contained component content（g）Represent, individual in adsorption process, the pH value of absorption system maintains 4.0.
Fig. 1 is the FTIR spectrum figure of Solid Modified pomelo peel powder；Infrared spectrogram threshold range is from 4000cm-1
To 500cm-1, as a result show in Solid Modified pomelo peel powder containing groups such as hydroxyl, carbonyl, alkyl.Fig. 2 is Solid Modified shaddock
The laser particle size analysis of sub- skin powder；Laser particle size analysis is carried out to Solid Modified pomelo peel powder, as a result shown, it is Solid Modified
The particle diameter distribution of the powder of pomelo peel has two peak values at 101.1 μm and 309.6 μm, and average particulate diameter exists
171.3μm.Fig. 3：Learn that it is straight that Solid Modified pomelo peel shows particle not of uniform size by SEM observation
Footpath, this is similar with the analysis result of laser particle analyzer, and amplifies after 12000 times, it can be seen that include in ultramicro powder structure
Small-bore, this is the architecture basics that pomelo peel carries out physical absorption to Epigallo-catechin gallate (EGCG).
Claims (3)
1. a kind of preparation method of pomelo peel-Epigallo-catechin gallate (EGCG) compound, it is characterised in that：With pomelo peel
For raw material, by different temperatures acid, alkali, ethanol, water elution, modified shaddock peel adsorbent is prepared into after drying, after being made
Adsorbent is mixed with Epigallo-catechin gallate (EGCG), by physics and Chemisorption, forms pomelo peel-table and does not have food
Sub- catechin and gallate compound.
2. a kind of preparation method of pomelo peel-Epigallo-catechin gallate (EGCG) compound according to claim 1,
It is characterized in that：Concretely comprise the following steps：
（1）Pomelo peel is dried to constant weight in 100-110 DEG C, 40 mesh sieves are crossed after crushing and obtain pomelo peel powder；
（2）Pomelo peel powder is positioned over and added in the Buchner funnel of No. 4 filter paper of Whatman, the NaOH for adding 0.1mol/L is molten
Liquid, carries out suction filtration, to filtrate pH stable, adds pure water and eluted；
（3）By step（2）Obtained pomelo peel powder, which is replaced in, to be added in the Buchner funnel of No. 4 filter paper of Whatman, is added
0.1mol/L HCl solution, carries out suction filtration, to filtrate pH stable, adds pure water and eluted；
（4）By step（3）Obtained pomelo peel powder, which is replaced in, to be added in the Buchner funnel of No. 4 filter paper of Whatman, is added
Ethanol solution, carries out suction filtration, adds pure water and eluted；
（5）By step（4）Obtained pomelo peel powder carries out frozen drying, obtains Solid Modified pomelo peel powder；
（6）With distilled water rinse solid pomelo peel powder, the solid pomelo peel powder after rinse is obtained, 10 g/L table is not had
Infanticide catechin and gallate is mixed with the pomelo peel powder after rinse, and adsorption reaction is carried out at 25 DEG C；
（7）After 8 hours, it is up to the composite body of adsorption equilibrium and lies at -20 DEG C and be freeze-dried, obtains solid forms
Pomelo peel-Epigallo-catechin gallate (EGCG) compound.
3. a kind of preparation method of pomelo peel-Epigallo-catechin gallate (EGCG) compound according to claim 2,
It is characterized in that：Step（6）Middle Epigallo-catechin gallate (EGCG) is with the pomelo peel powder after rinse according to volume mass
Than 1:4 ratio mixing.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710325956.2A CN107125653A (en) | 2017-05-10 | 2017-05-10 | The preparation method of pomelo peel Epigallo-catechin gallate (EGCG) compound |
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| CN201710325956.2A CN107125653A (en) | 2017-05-10 | 2017-05-10 | The preparation method of pomelo peel Epigallo-catechin gallate (EGCG) compound |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107535657A (en) * | 2017-10-24 | 2018-01-05 | 福建农林大学 | A kind of health protection tea of the powder containing Phyllanthus embical fruit and preparation method thereof |
| CN110250444A (en) * | 2019-07-26 | 2019-09-20 | 福建农林大学 | It is a kind of to contain bamboo shoot fiber-polyphenol complex sausage and preparation method thereof |
| CN110292071A (en) * | 2019-07-26 | 2019-10-01 | 福建省农业科学院农业工程技术研究所 | A kind of Yoghourt and preparation method thereof containing citrus peel fibres and tea polyphenols |
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| CN1457689A (en) * | 2003-05-15 | 2003-11-26 | 高景曦 | Function food with diet fibre and anthocyanidin |
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| CN104604977A (en) * | 2013-12-31 | 2015-05-13 | 刘葵 | Application of peel powder of citrus plants as filler in food processing |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107535657A (en) * | 2017-10-24 | 2018-01-05 | 福建农林大学 | A kind of health protection tea of the powder containing Phyllanthus embical fruit and preparation method thereof |
| CN110250444A (en) * | 2019-07-26 | 2019-09-20 | 福建农林大学 | It is a kind of to contain bamboo shoot fiber-polyphenol complex sausage and preparation method thereof |
| CN110292071A (en) * | 2019-07-26 | 2019-10-01 | 福建省农业科学院农业工程技术研究所 | A kind of Yoghourt and preparation method thereof containing citrus peel fibres and tea polyphenols |
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Application publication date: 20170905 |
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