CN107121208A - The data acquisition device and lifetime of excited state measuring method of lifetime of excited state measurement based on frequency counting card - Google Patents
The data acquisition device and lifetime of excited state measuring method of lifetime of excited state measurement based on frequency counting card Download PDFInfo
- Publication number
- CN107121208A CN107121208A CN201710427606.7A CN201710427606A CN107121208A CN 107121208 A CN107121208 A CN 107121208A CN 201710427606 A CN201710427606 A CN 201710427606A CN 107121208 A CN107121208 A CN 107121208A
- Authority
- CN
- China
- Prior art keywords
- lifetime
- light
- excited state
- counting card
- frequency counting
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 230000005281 excited state Effects 0.000 title claims abstract description 42
- 238000005259 measurement Methods 0.000 title claims abstract description 39
- 238000000034 method Methods 0.000 title claims abstract description 24
- 230000005284 excitation Effects 0.000 claims abstract description 46
- 238000004020 luminiscence type Methods 0.000 claims abstract description 25
- 238000012360 testing method Methods 0.000 claims abstract description 25
- 238000013016 damping Methods 0.000 claims abstract description 9
- 230000003287 optical effect Effects 0.000 claims description 15
- 238000005424 photoluminescence Methods 0.000 claims description 8
- 238000001914 filtration Methods 0.000 claims description 7
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims description 2
- 210000001367 artery Anatomy 0.000 claims 1
- 238000012512 characterization method Methods 0.000 claims 1
- 230000013011 mating Effects 0.000 claims 1
- 210000003462 vein Anatomy 0.000 claims 1
- 238000001514 detection method Methods 0.000 abstract description 7
- 230000035945 sensitivity Effects 0.000 abstract description 3
- 238000001161 time-correlated single photon counting Methods 0.000 description 8
- 238000005516 engineering process Methods 0.000 description 5
- 238000001857 fluorescence decay curve Methods 0.000 description 4
- 238000011160 research Methods 0.000 description 3
- 239000000463 material Substances 0.000 description 2
- 238000000691 measurement method Methods 0.000 description 2
- 241001269238 Data Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 238000013480 data collection Methods 0.000 description 1
- 230000001934 delay Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000009738 saturating Methods 0.000 description 1
- 238000007619 statistical method Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J11/00—Measuring the characteristics of individual optical pulses or of optical pulse trains
Landscapes
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The data acquisition device and lifetime of excited state measuring method of lifetime of excited state measurement based on frequency counting card, measuring method comprise the following steps：Excite testing sample to produce luminescence generated by light with the pulse laser of high repetition frequency, detect the luminescence generated by light signal of the sample, the photon time difference measured is divided into each time interval using the time interval between the detected photonic pulsed signals of frequency counting card measurement and excitation light pulse signal, the photon with different time difference data is dispensed into corresponding time zone；Photon in each time interval is counted respectively, its luminescence generated by light photon luminous intensity attenuation curve can be obtained, the lifetime of excited state that exponential damping fitting obtains testing sample is carried out to the intensity profile of light emission.Compare other fluorescence or phosphorescent lifetime measuring method, and the measurable lifetime of excited state data area of the inventive method is wider, and cost is lower；Simultaneously also with very high time resolution and detection sensitivity.
Description
Technical field
The invention belongs to lifetime of excited state field of measuring technique, specifically, it is related to a kind of swashing based on frequency counting card
Send out state lifetime measurement method and device.
Background technology
In scientific research field, the lifetime of excited state measurement of sample is the structure of matter and physics of the various molecules of research or material
One of important means of chemical property etc.；Especially in the field such as life science and material science, institute's study sample is carried out glimmering
Light lifetime measurement is detection method most conventional in the field scientific research.At present, measurement sample excitation state life-span (including fluorescence
Or phosphorescent lifetime) method mainly have following several：1) using the single photon counting technology (TCSPC) of time correlation, with reference to special
The TCSPC cards and highly sensitive photoelectric detector of door are measurable fluorescence lifetime；2) using gate photon counting technique, repeated measurement
Different delays time after each excitation pulse, the fluorescence signal intensity in same time width (gate-width), then can obtain its fluorescence
Attenuation curve；3) use high-speed photodetector and can directly gather the attenuation curve of fluorescence signal with reference to oscillograph.
In above-mentioned 3 kinds of methods, first method (i.e. single photon counting technology) has very high fluoroscopic examination sensitivity,
And its time resolution is also higher (can be to 100 psecs), therefore it is well suited for time of measuring yardstick shorter (such as hundred psecs is received to hundred
Second level) fluorescence lifetime.However, the fluorescence lifetime measurement device based on this method is required for purchase to hold high very much in the market
Expensive equipment, such as special TCSPC cards and its software kit；And these special TCSPC cards are due to laser triggering frequency
Limit and (typically required that repetition rate have to be larger than hundreds of KHz), it is shorter (such as most long that former capital is only used for time of measuring yardstick
To hundreds of nanoseconds) fluorescence lifetime.To two kinds of measuring methods later, cause its detection sensitive due in measuring principle
Degree and time resolution are only used for the fluorescent/phosphorescent life-span of time of measuring yardstick longer (such as more than Microsecond grade) all than relatively low,
But it is unsuitable in very low and the life-span shorter (such as nanosecond) the fluorescent samples of measurement photoluminescence intensity.
Found by substantial amounts of experiment and practice：Time interval between photonic pulsed signals and excitation light pulse signal is made
The photoluminescence intensity and the fluorescent samples in life-span for measuring wide scope can be used as photon time difference.
The content of the invention
In order to solve the confinement problems of existing lifetime of excited state measurement, the present invention provides following technical scheme：
A kind of data acquisition device of the lifetime of excited state measurement based on frequency counting card, described device includes pulse excitation
Light source, sample cell, fluorescence gathering system, the light outlets and fluorescence gathering system of the excitation source are towards sample cell, the collection
Device：Also include data collecting system, the data collecting system includes frequency counting card, and it is connected to pulse excitation light source
Signal synchronism output pin, and be connected with the signal output pin of the photoelectric detector of light collector.
Further, described device also includes light collector, the light outlets of the excitation source and sample cell center and
Light collector is in a straight line.
The fluorescence gathering system includes following each components that longitudinal arrangement is placed successively：Receive optical lens, notch filtering light piece,
Arrowband fluorescent optical filter, convergent lens and photoelectric detector, and the center of each component and the line at sample cell center are in a straight line,
The direction of the line is with exciting optical propagation direction vertical.
Further, each component is all enclosed in the seal closure of an internal blacking.
Further, the data collecting system also includes computer, the synchronization output signal and light of pulse excitation light source
The output signal of photodetector is transferred to frequency counting card by standard BNC lines respectively, between frequency counting card and computer
It is connected using USB data line.
A kind of lifetime of excited state measuring method based on frequency counting card, step is：
1) excite testing sample to produce luminescence generated by light with the pulse laser of repetition rate；
2) the luminescence generated by light signal of the testing sample, the detected photon pulse of frequency of use numbered card measurement are detected
Time interval between signal and excitation light pulse signal is poor as photon time；
3) measured photon time difference is divided into each time interval, by the photon with different time difference data
It is dispensed into corresponding time interval；
4) photon in each time interval is counted respectively, obtains luminescence generated by light number of photons with retouching of changing of time difference
State；
5) exponential damping fitting is made to the description that measured obtained luminescence generated by light number of photons changes with the time difference, is obtained
The lifetime of excited state of testing sample.
Further, the luminescence generated by light number of photons with the time difference change be described as luminous intensity attenuation curve, to institute
Measure obtained luminous intensity attenuation curve and make exponential damping fitting, obtain the lifetime of excited state of testing sample.
Further, the repetition rate of the pulse excitation light source is adjustable；It is this that the testing sample, which produces luminescence generated by light,
Fluorescence or phosphorescence that testing sample is sent after excitation state is transitted to.
Further, the repetition frequency range of the pulse laser of the repetition rate is 1KHz~20MHz.
Beneficial effect：Time interval between discovery photonic pulsed signals and excitation light pulse signal is used as photon time
Difference can be poor in order to time of measuring as the fluorescent samples that measurement photoluminescence intensity is very low and the life-span is shorter, in data
In harvester, Usage data collection system, i.e., the signal synchronism output that pulse excitation light source is connected to frequency counting card draws
Pin, and be connected with the signal output pin of the photoelectric detector of light collector, it is with this, two signal acquisitions are described to obtain
Time difference, measured for lifetime of excited state.And the measuring method in the present invention is used, and by the time difference signal acquisition, and it is right
Exponential damping fitting is made in the description of time difference change, can measure lifetime of excited state measurement.
Brief description of the drawings
Fig. 1 is the structural representation of the lifetime of excited state measurement apparatus of the present invention.
Fig. 2 is the excitation state fluorescence decay curve of the short life sample measured using the measurement apparatus.
Fig. 3 is the excitation state fluorescence decay curve of the long-life sample measured using the measurement apparatus.
Wherein：1st, pulse excitation light source, 2, sample cell, 3, light collector, 4, fluorescence gathering system, 5, receive optical lens,
6th, notch filtering light piece, 7, arrowband fluorescent optical filter, 8, convergent lens, 9, photoelectric detector, 10, frequency counting card, 11, calculate
Machine.
Embodiment
Embodiment 1：A kind of data acquisition device of the lifetime of excited state measurement based on frequency counting card, described device includes
Pulse excitation light source 1, sample cell 2, fluorescence gathering system 4, the light outlets and fluorescence gathering system of the excitation source 1 are towards sample
Product pond, in order to obtain the time interval between photonic pulsed signals and excitation light pulse signal, the device also includes data
Acquisition system, the data collecting system includes frequency counting card, and its signal synchronism output for being connected to pulse excitation light source 1 draws
Pin, and be connected with the signal output pin of the photoelectric detector of light collector 3.
Described device also includes light collector, light outlets and the center of sample cell 2 and the ray-collecting of the excitation source 1
Device 3 is in a straight line.The purpose is to be collected using light collector to remaining laser, and this kind of position so that sample cell, which is received, to swash
Light is more abundant, it also avoid detection of the residual excitation light to fluorescent and interferes.
The fluorescence gathering system 4 includes following each components that longitudinal arrangement is placed successively：Receive optical lens 5, notch filtering light
Piece 6, arrowband fluorescent optical filter 7, convergent lens 8 and photoelectric detector 9, and the line at the center and the center of sample cell 2 of each component
In a straight line, the direction of the line is with exciting optical propagation direction vertical.This kind of fluorescence gathering system proposed, for laser pick-off
There is well adapting to property with processing, obtain more accurate for time difference signal.And be vertically arranged and further avoid
Detection of the residual excitation light to fluorescent is interfered.
In order to reduce ambient noise, each component is all enclosed in the seal closure of an internal blacking,
In one embodiment, the data collecting system also includes computer 11, the synchronism output of pulse excitation light source 1
The output signal of signal and photoelectric detector 9 is transferred to frequency counting card 10, frequency counting card 10 by standard BNC lines respectively
It is connected between computer 11 using USB data line, now, the data acquisition device has become one and is applied to measurement
In a computer the measurement apparatus of fluorescence lifetime, i.e., handled gathered data, relates generally to statistics and fitting, successively
Fluorescence lifetime can be obtained.
In one embodiment, the disclosure records a kind of lifetime of excited state measuring method based on frequency counting card, comprising
Following steps：
1) excite testing sample to produce luminescence generated by light with the pulse laser of repetition rate；The weight of the pulse excitation light source
Complex frequency is adjustable；It is the fluorescence or phosphorus that the testing sample is sent after excitation state is transitted to that the testing sample, which produces luminescence generated by light,
Light.
2) the luminescence generated by light signal of the testing sample, the detected photon pulse of frequency of use numbered card measurement are detected
Time interval between signal and excitation light pulse signal is poor as photon time；
3) measured photon time difference is divided into each time interval, by the photon with different time difference data
It is dispensed into corresponding time interval；
4) photon in each time interval is counted respectively, obtains luminescence generated by light number of photons with retouching of changing of time difference
State；
5) exponential damping fitting is made to the description that measured obtained luminescence generated by light number of photons changes with the time difference, is obtained
The lifetime of excited state of testing sample.In this step, can using specific explanations as：The luminescence generated by light number of photons changes with the time difference
Be described as luminous intensity attenuation curve, measured obtained luminous intensity attenuation curve is made exponential damping fitting, obtain
The lifetime of excited state of testing sample.
Embodiment 2：The purpose of the present embodiment is to provide a kind of compatible very wide life span (such as hundred picoseconds to millisecond
Level) and the relatively inexpensive lifetime of excited state measuring method of price and device, you can for measuring short-life fluorescent samples, it is also possible to
In long-life phosphorescence sample.
Lifetime of excited state measuring method based on frequency counting card, includes following steps：
1) excite testing sample to produce luminescence generated by light with the pulsed light of high repetition frequency；
2) the luminescence generated by light signal of the sample is detected, detected photonic pulsed signals are measured using frequency counting card
Time interval (time difference) between excitation light pulse signal；
3) the photon time difference measured is divided into each time interval, by the photon with different time difference data point
In the corresponding time interval of supplying；
4) photon in each time interval is counted respectively, its luminescence generated by light number of photons can be obtained with the time difference
Change (i.e. luminous intensity attenuation curve)；
5) exponential damping fitting is carried out to the photoluminescence intensity curve measured, you can obtain the excitation state of testing sample
Life-span.
The repetition rate of the pulse excitation light source requires adjustable；The testing sample requirement can produce luminescence generated by light, i.e.,
The sample can send fluorescence or phosphorescence after excitation state is transitted to.
As shown in figure 1, the fluorescence lifetime measurement device for implementing above-mentioned measuring method, mainly includes：Pulse excitation light
Source 1, sample cell 2, light collector 3, fluorescence gathering system 4 and data collecting system.
Wherein, the fluorescence gathering system is by the receipts optical lens 5 set gradually, notch filtering light piece 6, arrowband fluorescent optical filter
7, convergent lens 8 and photoelectric detector 9 are constituted.The light outlets of excitation source 1 are in the center of sample cell 2, the position of light collector 3
One straight line；More precisely, exciting light should finally enter light collector 3 by sample cell center, to avoid residual excitation light
Detection to fluorescent is interfered.Each component center and sample cell center are in a straight line in fluorescence gathering system, direction with
Excite optical propagation direction vertical；And its each component is all enclosed in the seal closure of an internal blacking, to reduce ambient noise.
The data collecting system includes a frequency counting card 10 and a computer 11.The synchronism output of excitation source
The output signal of signal and photoelectric detector is connected to frequency counting card by standard BNC lines respectively, and frequency counting card is with counting
It is connected between calculation machine using USB data line.
The main composition structure of the lifetime of excited state measurement apparatus provided above for the present embodiment, in order to more clearly understand
The lifetime measurement device, is described further to its operation principle below.
In actual lifetime of excited state measurement process, pulse excitation light source is first turned on, and appropriate repetition rate is set,
Then luminescence generated by light signal of the testing sample produced by after being excited is detected by fluorescence gathering system and is converted into telecommunications
Number.Time difference between all photonic pulsed signals detected excitation pulse signal corresponding to its will be born by frequency counting card
Duty is measured and gathered, and these time difference datas measured are sent into computer by USB data line afterwards, is finally being calculated
It is uniformly processed in machine using data processor：I.e. by the data distribution of these plenty of time of statistical analysis difference, to obtain
Obtain photoluminescence intensity to change with time (i.e. luminous intensity attenuation curve), final fitting obtains lifetime of excited state.
Relative to existing fluorescent/phosphorescent lifetime measurement technology, the scheme of the disclosure has following advantages：
1st, the skill of waveform is directly gathered relative to traditional gate photon counting technique and high-speed photodetector+oscillograph
Art, the present invention is poor (precision is up to 50ps) come time of measuring by frequency counting card, with very high time resolution, therefore can be used for
Measure the lifetime of excited state of short-term time scale.
2nd, relative to traditional fluorescent/phosphorescent lifetime measurement technology, the present invention is excited using dim light, will not damage sample,
Also it can be measured in the case of very low sample concentration, singl e photon detection can be accomplished in principle；And ambient noise disturbs small, signal to noise ratio
Height, causes lifetime of excited state measurement accuracy high.
3rd, compared with fluorescence lifetime measurement instrument of the in the market based on TCSPC cards, the present invention is with same fluoroscopic examination spirit
Sensitivity and time resolution, it can also be used to measure the fluorescence lifetime of short-term time scale (such as hundred psecs to hundred nanoseconds)；But the present invention
The special TCSPC cards due to not using, it is not necessary to limit exciting light triggering frequency, therefore can also be used to measure swashing for long time scale
Send out the state life-span.Therefore, measurable non-constant width of lifetime of excited state scope (such as may span across hundred picoseconds to millisecond magnitude) of the invention.
4th, compared with fluorescence lifetime measurement instrument of the in the market based on TCSPC cards, the present invention uses common frequency counting card,
And use existing data acquisition and procession program, relative to special TCSPC cards and related software, in cost can significantly under
Drop.
Embodiment 3：For investigation of the lifetime of excited state measurement apparatus of the present invention when measuring short life sample, adopt
Tested with No. 1 short life sample (its photoluminescence peak position is about in 610nm).The cuvette containing No. 1 sample is put first
Enter in sample cell, sample is excited using picosecond pulse laser (405nm), and set laser repetition rate to be 20MHz；Using
405nm notch filtering light pieces, fluorescent optical filter is 610nm narrow band pass filters, receives light and convergent lens and uses 60mm focal length lenses,
The fluorescence decay curve of the measured sample is as shown in Figure 2.Using single exponent ring-down function I (t)=I0exp(-t/τ)+c
(τ is the fluorescence lifetime of testing sample in formula, and c is ambient noise) carries out data fitting to the attenuation curve, can obtain this to be measured
The fluorescence lifetime of sample is about τ=6.37ns.
Embodiment 4：For lifetime of excited state measurement apparatus of the present invention examining when measuring long-life phosphors sample
Examine, tested using No. 2 long-life samples (its photoluminescence peak position is about in 560nm).First the colorimetric containing No. 2 samples
Ware is put into sample cell, sample is then excited using 405nm pulse lasers, and set laser repetition rate to be 1KHz；Adopt
With 405nm notch filtering light pieces, fluorescent optical filter is 560nm narrow band pass filters, receives light and convergent lens saturating using 60mm focal lengths
Mirror, the fluorescence decay curve of the measured sample is as shown in Figure 3.Using single exponent ring-down function I (t)=I0exp(-t/τ)+
C (τ is the fluorescence lifetime of testing sample in formula, and c is ambient noise) carries out data fitting to the attenuation curve, can obtain this to be measured
The fluorescence lifetime of sample is about 52.0 μ s.
In the various embodiments described above, laser repetition rate can use 0.05MHz, 3MHz etc. in 1KHz-20MHz scope
Interior to realize, certainly, laser repetition rate is not limited to this scope, sets according to the actual requirements, in the present embodiment scope
Laser repetition rate it is more preferable for the measure accuracy of fluorescence lifetime.
It is described above, only the invention preferably embodiment, but the invention protection domain not
This is confined to, any one skilled in the art is in the technical scope that the invention is disclosed, according to the present invention
The technical scheme of creation and its inventive concept are subject to equivalent substitution or change, should all cover the invention protection domain it
It is interior.
Claims (9)
1. a kind of data acquisition device of the lifetime of excited state measurement based on frequency counting card, described device includes pulse excitation light
Source (1), sample cell (2), fluorescence gathering system (4), the light outlets and fluorescence gathering system of the excitation source (1) are towards sample
Pond, it is characterised in that：Also include data collecting system, the data collecting system includes frequency counting card, and it is connected to pulse
The signal synchronism output pin of excitation source (1), and connect with the signal output pin of the photoelectric detector of light collector (3)
Connect.
2. the data acquisition device of the lifetime of excited state measurement according to claim 1 based on frequency counting card, its feature
It is, described device also includes light collector, light outlets and sample cell (2) center and light of the excitation source (1) are received
Storage (3) is in a straight line.
3. the data acquisition device of lifetime of excited state measurement according to claim 1, it is characterised in that：The phosphor collection
System (4) includes following each components that longitudinal arrangement is placed successively：Receive optical lens (5), notch filtering light piece (6), the filter of arrowband fluorescence
Mating plate (7), convergent lens (8) and photoelectric detector (9), and the center of each component and the line at sample cell (2) center are in always
Line, the direction of the line is with exciting optical propagation direction vertical.
4. the data acquisition device of lifetime of excited state measurement according to claim 3, it is characterised in that：Each component is complete
Portion is enclosed in the seal closure of an internal blacking.
5. the data acquisition device of lifetime of excited state measurement according to claim 1, it is characterised in that：The data acquisition
System also includes computer (11), the synchronization output signal of pulse excitation light source (1) and the output signal of photoelectric detector (9) point
Frequency counting card (10) is not transferred to by standard BNC lines, USB numbers are used between frequency counting card (10) and computer (11)
It is connected according to line.
6. a kind of lifetime of excited state measuring method based on frequency counting card, its characterization step is：
1) excite testing sample to produce luminescence generated by light with the pulse laser of repetition rate；
2) the luminescence generated by light signal of the testing sample, the detected photonic pulsed signals of frequency of use numbered card measurement are detected
Time interval between excitation light pulse signal is poor as photon time；
3) measured photon time difference is divided into each time interval, the photon with different time difference data is distributed
Enter in corresponding time interval；
4) photon in each time interval is counted respectively, obtains the description that luminescence generated by light number of photons changes with the time difference；
5) exponential damping fitting is made to the description that measured obtained luminescence generated by light number of photons changes with the time difference, obtains to be measured
The lifetime of excited state of sample.
7. the lifetime of excited state measuring method according to claim 6 based on frequency counting card, it is characterised in that the light
Photoluminescence number of photons with the time difference change be described as luminous intensity attenuation curve, measured obtained luminous intensity is decayed bent
Line makes exponential damping fitting, obtains the lifetime of excited state of testing sample.
8. the lifetime of excited state measuring method according to claim 1 based on frequency counting card, it is characterised in that：The arteries and veins
The repetition rate of impulse light emitting source is adjustable；The testing sample generation luminescence generated by light is the testing sample after excitation state is transitted to
The fluorescence or phosphorescence sent.
9. the lifetime of excited state measuring method according to claim 1 based on frequency counting card, it is characterised in that：It is described heavy
The repetition frequency range of the pulse laser of complex frequency is 1KHz~20MHz.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710427606.7A CN107121208A (en) | 2017-06-08 | 2017-06-08 | The data acquisition device and lifetime of excited state measuring method of lifetime of excited state measurement based on frequency counting card |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710427606.7A CN107121208A (en) | 2017-06-08 | 2017-06-08 | The data acquisition device and lifetime of excited state measuring method of lifetime of excited state measurement based on frequency counting card |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107121208A true CN107121208A (en) | 2017-09-01 |
Family
ID=59729643
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710427606.7A Withdrawn CN107121208A (en) | 2017-06-08 | 2017-06-08 | The data acquisition device and lifetime of excited state measuring method of lifetime of excited state measurement based on frequency counting card |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107121208A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108007584A (en) * | 2017-11-08 | 2018-05-08 | 南京邮电大学 | Rapid fluorescence service life imaging method based on single photon avalanche diode detector |
| CN111664951A (en) * | 2019-03-06 | 2020-09-15 | 中国科学院大连化学物理研究所 | Picosecond resolution single photon weak signal measuring device and measuring method |
| CN111712705A (en) * | 2018-02-16 | 2020-09-25 | 莱卡微系统Cms有限责任公司 | Fluorescence lifetime-microscopy-method using time-dependent single photon counting |
| CN113203487A (en) * | 2021-03-18 | 2021-08-03 | 深圳大学 | Quantitative correction method and device for fluorescence lifetime deviation |
-
2017
- 2017-06-08 CN CN201710427606.7A patent/CN107121208A/en not_active Withdrawn
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108007584A (en) * | 2017-11-08 | 2018-05-08 | 南京邮电大学 | Rapid fluorescence service life imaging method based on single photon avalanche diode detector |
| CN108007584B (en) * | 2017-11-08 | 2019-10-11 | 南京邮电大学 | Rapid fluorescence service life imaging method based on single photon avalanche diode detector |
| CN111712705A (en) * | 2018-02-16 | 2020-09-25 | 莱卡微系统Cms有限责任公司 | Fluorescence lifetime-microscopy-method using time-dependent single photon counting |
| CN111664951A (en) * | 2019-03-06 | 2020-09-15 | 中国科学院大连化学物理研究所 | Picosecond resolution single photon weak signal measuring device and measuring method |
| CN111664951B (en) * | 2019-03-06 | 2021-10-15 | 中国科学院大连化学物理研究所 | Picosecond resolution single photon weak signal measuring device and measuring method |
| CN113203487A (en) * | 2021-03-18 | 2021-08-03 | 深圳大学 | Quantitative correction method and device for fluorescence lifetime deviation |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107121208A (en) | The data acquisition device and lifetime of excited state measuring method of lifetime of excited state measurement based on frequency counting card | |
| Smedley et al. | Determining the K-content of single-grains of feldspar for luminescence dating | |
| US8325337B2 (en) | Time resolved raman spectroscopy | |
| CN100590422C (en) | Water body pollution laser induction fluorescence remote-measuring method | |
| JP4397692B2 (en) | System and method for multi-photon counting with time correlation | |
| CN103728446B (en) | Photon counting-type hyperchannel time resolved fluoro-immunoassay system and method for counting | |
| Harris et al. | Measurement of subnanosecond fluorescence decays by sampled single‐photon detection | |
| US20060243911A1 (en) | Measuring Technique | |
| AU769370B2 (en) | Method and apparatus for spectrometric analysis of turbid, pharmaceutical samples | |
| CN107003239B (en) | Self-triggering flow cytometer | |
| EP0420944A1 (en) | Optical inspection of food products | |
| CN101196471A (en) | Soil heavy metal pollution quantification detecting system and detecting method | |
| EP2362207A1 (en) | Measuring system and method, in particular for determining blood sugar | |
| CN104964964A (en) | Portable laser raman spectrometer based on prismatic decomposition | |
| CN106093962B (en) | A kind of interference velocity-measuring system and method | |
| CN108463714A (en) | The emission lifetime measurement method and equipment of average life span for measuring excited electronic state | |
| CN202049112U (en) | Pearl quality detecting device | |
| CN106290286A (en) | Time-resolved fluorescence assay system and detection device | |
| CN107643272A (en) | A kind of time-resolved fluorescence measuring system based on few passage TCSPC and multi-detector | |
| CN206804165U (en) | The data acquisition device and the measurement apparatus of lifetime of excited state measurement based on frequency counting card | |
| CN106970058A (en) | The minimal feeding instrument and detection method in a kind of pair of fluorescent emission face | |
| McCoy et al. | Some aspects of single-grain luminescence dating | |
| Sabol et al. | Absolute fluorescence quantum yields by relative fluorescence and photoacoustic measurements of low level luminescence quenching | |
| Moger et al. | The application of fluorescence lifetime readouts in high-throughput screening | |
| CN208902611U (en) | Thin film damage discriminating gear based on the plasma ignition time |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20170901 |
|
| WW01 | Invention patent application withdrawn after publication |