CN107119134B - Chimonanthus chinensis EST-SSR marker and application thereof in genetic diversity analysis - Google Patents

Abstract

The invention provides a development method of endangered plant chimonanthus nitens EST-SSR molecular marker, which mainly comprises the following steps: the method comprises the EST sequence of calyx canthus, identification and screening of SSR sites and the sequence of EST-SSR molecular marker primers. In the invention, 200 sequences containing different repeat units are searched from Chimonanthus praecox transcriptome data, and 23 EST-SSR markers with rich polymorphism are obtained by combining software analysis and molecular marker screening. The method can be applied to genetic diversity analysis, germplasm identification, variation and evolution research, molecular marker assisted breeding and the like among different species and populations of the endangered plant Chimonanthus praecox. Good repeatability and high reliability, and is a very effective molecular marker.

Description

Chimonanthus chinensis EST-SSR marker and application thereof in genetic diversity analysis

Technical Field

The invention relates to the field of biotechnology, in particular to a molecular marker technology based on transcriptome high-throughput sequencing of the whole wintersweet.

Background

The Chimonanthus chinensis (Cheng et S.Y. Chang) belongs to Chimonanthus chinensis of Chimonadaceae, is a third-class wiggery plant, is mainly distributed in Zhejiang province (Lingan city and Tiantai county) in China, belongs to a second-class important protection frequent species in China, and has great scientific research value in plant system research. The calyx canthus is characterized by dicotyledonous plants, shrubs, stem clusters, flower tops, non-scale buds and the like, and has great ornamental value and medicinal value. The wild wintersweet resources are few, and the living environment of the wintersweet is influenced by natural disasters and human interference and is divided into a plurality of small populations, so that different populations of the wintersweet are isolated at different distances, gene exchange among the populations is reduced, the internal inbreeding rate is increased, the genetic diversity in the populations is reduced, and the genetic differentiation among the populations is increased. Therefore, the effective identification and local protection of the summer wintersweet germplasm resources are urgently needed.

Simple Sequence Repeat (SSR) is a 1-6 nucleotide tandem repeat unit widely distributed in eukaryotic and prokaryotic genomes, such as (GA)_n，(AC)_n，(GAA)_nEtc., the same type of SSR can be distributed over different locations in the genome, forming polymorphisms at each locus due to their varying number and degree of repetition. Although various markers (e.g., isozymes, RAPD, ISSR, and AFLP) have been used for genetic diversity and mating system analysis, the polymorphic information revealed by these markers is generally low and does not reflect the genetic diversity and genetic differentiation characteristics between species well. Compared with the molecular marker technologies, the SSR marker has the advantages of abundant polymorphic information, codominant inheritance, strong specificity and the like. Therefore, it is necessary to develop a codominant SSR molecular marker primer for related researches such as genetic diversity and variety identification protection of Chimonanthus praecox.

At present, relatively few SSR primer development and related genetic research reports are reported for Chimonanthus nitens, and the related researches such as genetic diversity analysis, variety identification and protection and the like of the Chimonanthus nitens are greatly limited.

Disclosure of Invention

The invention aims to provide an effective EST-SSR molecular marker of Chimonanthus nitens and application thereof.

The prune EST-SSR marker comprises 23 microsatellite markers, and the number, nucleotide sequence, primer sequence and characteristics of the microsatellite markers are shown in the following table:

the sequence numbers of the nucleotide sequence and the primer sequence are the numbers in the sequence table.

A Chimonanthus nitens EST-SSR marker comprises the following steps:

(1) wintersweet transcriptome sequencing;

(2) screening and identifying SSR sequences to obtain 23 microsatellite markers;

(3) designing and synthesizing SSR primers, and randomly selecting a primer 220 pair of 2-6 nucleotide repetitive motifs;

(4) screening SSR primers to obtain 23 pairs of primers;

wherein, the 23 microsatellite markers, the numbers, the nucleotide sequences and the 23 primer sequences are adopted.

The invention relates to application of a calyx canthus EST-SSR marker in genetic diversity analysis.

The invention has the beneficial effects that:

the SSR markers can be divided into genome SSR (gSSR) and transcriptome SSR (EST-SSR), and compared with the gSSR markers, the EST-SSR markers obtained from the EST database are more economical and efficient to establish. Therefore, the invention provides an effective EST-SSR molecular marker of the Chimonanthus chinensis and utilizes the established high-efficiency SSR-PCR system of the Chimonanthus chinensis to carry out genetic diversity analysis on different levels. The invention mainly comprises the following steps: the method comprises the EST sequence of calyx canthus, identification and screening of SSR sites and the sequence of EST-SSR molecular marker primers. The invention searches 200 sequences containing different repeat units from Chimonanthus praecox transcriptome data, and obtains 23 EST-SSR markers with rich polymorphism by combining software analysis and molecular marker screening, wherein the EST-SSR markers are respectively as follows: XLM001, XLM003, XLM004, XLM007, XLM020, XLM021, XLM023, XLM035, XLM046, XLM061, XLM075, XLM076, XLM078, XLM088, XLM089, XLM091, XLM095, XLM098, XLM100, XLM112, XLM118, XLM139, XLM 199. The method can be applied to genetic diversity analysis, germplasm identification, variation and evolution research, molecular marker assisted breeding and the like among different species and populations of the endangered plant Chimonanthus praecox. Good repeatability and high reliability, and is a very effective molecular marker.

The EST-SSR molecular marker of Chimonanthus nitens and the application thereof are further explained in the following by combining the attached drawings.

Drawings

FIG. 1: amplifying SSR graphs of 15 Chimonanthus nitens samples in 5 populations by XLM001 site specific primers;

FIG. 2: amplifying SSR graphs of 15 Chimonanthus nitens samples in 5 populations by using XLM003 site specific primers;

FIG. 3: amplifying SSR graphs of 15 Chimonanthus nitens samples in 5 populations by using XLM004 site specific primers;

FIG. 4: amplifying SSR graphs of 15 prunella vulgaris samples in 5 populations by using XLM007 site specific primers;

FIG. 5: amplifying SSR graphs of 15 prunella vulgaris samples in 5 populations by using XLM020 site specific primers;

FIG. 6: amplifying SSR graphs of 15 Chimonanthus nitens samples in 5 populations by XLM021 site specific primers;

FIG. 7: amplifying SSR graphs of 15 Chimonanthus nitens samples in 5 populations by using XLM023 site specific primers;

FIG. 8: amplifying SSR graphs of 15 prunella vulgaris samples in 5 populations by using XLM035 site specific primers;

FIG. 9: amplifying SSR graphs of 15 Chimonanthus nitens samples in 5 populations by XLM046 site specific primers;

FIG. 10: amplifying SSR graphs of 15 Chimonanthus nitens samples in 5 populations by XLM061 site specific primers;

FIG. 11: amplifying SSR (simple sequence repeat) graphs of 15 prunella vulgaris samples in 5 populations by using XLM075 site specific primers;

FIG. 12: amplifying SSR graphs of 15 prunella vulgaris samples in 5 populations by XLM076 site specific primers;

FIG. 13: amplifying SSR graphs of 15 prunella vulgaris samples in 5 populations by XLM078 site specific primers;

FIG. 14: amplifying SSR graphs of 15 Chimonanthus nitens samples in 5 populations by using XLM088 site specific primers;

FIG. 15: amplifying SSR graphs of 15 Chimonanthus nitens samples in 5 populations by using XLM089 site specific primers;

FIG. 16: amplifying SSR graphs of 15 prunella vulgaris samples in 5 populations by using XLM091 site specific primers;

FIG. 17: amplifying SSR graphs of 15 Chimonanthus nitens samples in 5 populations by XLM095 site specific primers;

FIG. 18: amplifying SSR graphs of 15 Chimonanthus nitens samples in 5 populations by XLM098 site specific primers;

FIG. 19: amplifying SSR graphs of 15 Chimonanthus nitens samples in 5 populations by XLM100 site specific primers;

FIG. 20: amplifying SSR graphs of 15 Chimonanthus nitens samples in 5 populations by XLM112 site specific primers;

FIG. 21: amplifying SSR graphs of 15 Chimonanthus nitens samples in 5 populations by using XLM118 site specific primers;

FIG. 22: amplifying SSR graphs of 15 Chimonanthus nitens samples in 5 populations by using XLM139 site specific primers;

FIG. 23: XLM199 site-specific primers were used to amplify SSR patterns of 15 Chimonanthus praecox samples in total in 5 populations.

Detailed Description

(1) Transcriptome sequencing

Collecting fresh tender leaf of wild Fraxinus chinensis 0.5g, wrapping with tinfoil paper, quickly freezing in liquid nitrogen, and storing at-80 deg.C. Total RNA of Chimonanthus praecox was extracted using RNAioso Plus (TaKaRa). Before sequencing, the concentration and quality of total RNA were determined using Nanodrop and Agilent 2100, OD260/280 and OD260/230 were between 1.8-2.2, 28S:18S>1.3，RIN>7, no degradation and no pollution. QUBIT RNA ASSAY KIT was used to accurately quantify the starting total RNA at 0.1-1. mu.g. Enriching and purifying mRNA in total RNA by using Beads with Oligod (T), adding fragmentation buffer to break the mRNA into short segments, and synthesizing a first cDNA chain by using a hexabasic random primer (randomhexamers) by using the mRNA as a template; then adding buffer solution, dNTPs, RNase H and DNA polymerase I to synthesize a second cDNA chain; purifying by a kit, adding EB buffer solution for elution, performing end repair, adding A and connecting a sequencing joint, performing fragment size selection by agarose gel electrophoresis, performing PCR amplification, and accurately quantifying a PCR product by QUBIT DNA HS ASSAY KIT; constructed sequencing library Illumina HiSeq^TM2000 in-silico sequencing was performed with three biological replicates.

(2) Screening and identification of SSR sequences

A script MISA (MicrosAtellite identification tool, http:// pgrc. ipk-gatesleben. de/missa /) written in perl language and capable of identifying simple Repeat sequences (SSR) from fasta sequences can effectively identify and identify SSR sequences and loci, and the parameter setting standard is as follows: the number of times of mononucleotide repeat is 15, the number of times of dinucleotide repeat is 6, and the number of times of three to six nucleotide repeat is 5; the length of the SSR locus flanking sequence is more than or equal to 150 bp. In this example, 23 microsatellite markers were selected and assigned the respective numbers XLM001, XLM003, XLM004, XLM007, XLM020, XLM021, XLM023, XLM035, XLM046, XLM061, XLM075, XLM076, XLM078, XLM088, XLM089, XLM091, XLM095, XLM098, XLM100, XLM112, XLM118, XLM139, XLM199, and the respective nucleotide sequences shown in SEQ ID NO 1-SEQ ID NO 23 of the sequence Listing.

(3) Design and synthesis of SSR primers

SSR primers were designed in batches using primer3(https:// sourceforce. net/targets/primer 3/) based on the sequences and sites detected in (2). The design criteria of the primers are as follows: there is no SSR in the primer sequences; the sequence is in a DNA conserved sequence region; the length is between 15 and 30 bp; the upstream and downstream primers do not have complementary sequences; the primers themselves do not have complementary sequences; the primer annealing temperature (Tm) is between 53 and 63 ℃; the difference of the Tm values of the upstream primer and the downstream primer is less than or equal to 5 ℃; the GC content is between 40% and 60%; the size of the product is 100-400 bp. Primer 220 pairs (No.: XLM001-XLM220) of 2-6 nucleotide repeat motifs were randomly selected, synthesized by Shanghai Sangon bioengineering, Inc., and used for SSR primer screening.

(4) Screening of SSR primers

Selecting 5 wild populations of chimonanthus salicifolius wild materials: the method comprises the steps of preparing 3 samples from a Zhejiang balcony through plateau (JGP) and Zhejiang mortar hole (DJK), Yuntangshan mountain (LTS) of Zhejiang Linan, Zhejiang West pit (XK), Zhejiang front pit (QK) and Longxushan (LXS) of Anhui, wherein each population is randomly selected, collecting fresh leaves, drying silica gel and storing at low temperature. Extracting genome DNA by CTAB method, detecting its quality by 0.8% agarose gel electrophoresis, and diluting DNA concentration to 75 ng. mu.L^-1Then storing at-20 ℃ for later use.

SSR-PCR reaction system adopts 20 mu L reaction system which comprises 75ng of templateDNA、250μmol·L^{- 1}dNTPs、1.5mmol·L^-1Mg²⁺0.6U TaqDNA polymerase, 0.2. mu. mol. L^-1Primers and 10 × PCR buffer. The SSR reaction program is as follows: pre-denaturation at 94 ℃ for 3 min; 30 cycles (denaturation at 94 ℃ for 30s, renaturation at 55-60 ℃ for 30s, extension at 72 ℃ for 1min) and extension at 72 ℃ for 10 min.

After the reaction is finished, 2 mu L of loading buffer is added into the product, 100bp DNA ladder is used as DNA molecular weight standard, 8% non-denatured polyacrylamide is adopted for electrophoresis, the electrophoresis buffer solution is 0.5 xTBE, 160V voltage stabilization electrophoresis is carried out for 90min, and a blue band is preferably used for running to a position which is about 1/3 away from the bottom of the polypropylene gel. After electrophoresis, the gel is dyed by a silver staining method, and finally the gel is placed on a gel imaging system for photographing. A primer for stably amplifying a clear target band according to the band amplification condition statistics; and counting the primers with different bands in the target range according to the band migration condition. In this example, 23 pairs of primers were obtained by screening, and the sequences of the primers are shown in SEQ ID NO. 24-SEQ ID NO. 69 of the sequence Listing.

(5) Application of SSR primers in different populations of Chimonanthus praecox and genetic diversity analysis

Selecting 5 wild populations of chimonanthus salicifolius wild materials: the method comprises the steps of selecting 3 samples from each population of Zhejiang sky terrace through plateau (JGP), Zhejiang mortar hole (DJK), Zhejiang Lin Anlongtang (LTS), Zhejiang West pit (XK) and Zhejiang forepit (QK), extracting genome DNA by a CTAB method, performing SSR amplification on 23 pairs of primers obtained by screening in the step (4), marking different classification band types of the bands of the sizes of the bands as A, B, C, D …, marking homozygote bands as AA, BB, CC and DD …, marking heterozygote bands as AB, BD, CD and BD … according to the band types, and using band type data for subsequent genetic analysis such as genetic diversity. SSR-PCR amplification is carried out on the 23 pairs of primers with the inter-population polymorphism, and the result shows that 12 pairs of primers amplify polymorphic bands: namely XLM003, XLM007, XLM021, XLM035, XLM046, XLM061, XLM078, XLM089, XLM095, XLM098, XLM100, XLM 139; the bands of amplification products of XLM007, XLM078, XLM098 and XLM139 in the population are rich, and the allelic variation is more. The test results are shown in FIGS. 1 to 23. Therefore, the method can be applied to genetic diversity analysis, germplasm identification, mutation and evolution research, molecular marker assisted breeding and the like among different species and populations of the endangered plant Chimonanthus praecox. Good repeatability and high reliability, and is a very effective molecular marker.

TABLE 1 primer characterization sequence Listing

The above-mentioned embodiments are merely illustrative of the preferred embodiments of the present invention, and do not limit the scope of the present invention, and various modifications and improvements of the technical solution of the present invention by those skilled in the art should fall within the protection scope defined by the claims of the present invention without departing from the spirit of the present invention.

SEQUENCE LISTING

<110> Zhejiang agriculture and forestry university

<120> summer wax plum EST-SSR marker and application thereof in genetic diversity analysis

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<170>PatentIn version 3.5

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agggttaaat agatgcgaat acatgctaaa acagtgataa gtcccctcac ctggtgtgca 300

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gccgggtatg ggaagttggg gcgagttgga aaaccgggac gctccggcaa aactgggacg 420

atccctcaag ttcagttgga aacgggcttg caaaaggatc cagaaacggt ccaagaacgc 480

ttcaagaacg atcccaaagc gggccacgaa taggcccaaa atgagcctgg atcaagcctg 540

aactgggcct aaaatggact tggaatgggc ctgggtcggg cctgaaaccg gctgggcctg 600

actggatctg ttgaattggg cttggatcag attgggccta aggctgctag gcccaagtgc 660

tggccaggga tcaaggttga cgagttgggc atggattgga cgggtaaggc ttagagctgg 720

gtccgaacgg gtcactctct tcctcccacg attcggctca gtgactcgac ttagtcaact 780

cgatgactca gttgagtcgc tcagtgaatc aaatgagtcg agtcgaagac tcaaaagaga 840

gggaaaagag agggaaaaga agatatcaag ggggaggaaa atggcagtgg agaggaagaa 900

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gaggggagga gaggagacgg cggcagcggc agcggcacaa gagatttagg gttttggtta 1080

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gaaagagaaa aggggcgcgg cttggaggct tagg 1174

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cttagattat ttccatgtag gagatactag agaatgatgc gacaaatgtt ttttttgtta 120

agtgtatgtt tagctttttc ccttactgat ggtccttgga tctttggata agttaaggaa 180

tcatggtgta aatgaaataa aagtaaatcc cattaatcca ttgttccatt agcaattttg 240

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atttgcgaga gagacaataa caaatcaatt gataccactt ctttcatatc ttcaaatgat 540

taggcaatat ttgagtctta cgatggtttt tctgcagtag cccaattaag ataaagtggc 600

accacaactt gatttgtagt ggatagttga cttcatcatc catatccaaa taatgtccat 660

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actgtactcc aatgtaaaaa gtggatttga agctctaagc tagaaattgt atacactgtc 840

atttgtcata accatgacgt ctatgttaaa aaatacttca aatttccaag cgaagagttt 900

caaagtagac atgcatatcc ttgtcatttc tcaccaataa tccccacaag aacagaaccc 960

atcattgaaa tggtgaaacc gattggtatc tctaacaaat atctctctcc cttcaaattt 1020

ggatataaat tttatcactg catgacagtc accacagatt cggaggttct taataactcg 1080

gagtggggtt ccaggtggag tgttcagaag gccaaggcca attgccaatt tctcactgtg 1140

cccacccaag atatgctcct tctcttgctc ctcaacatcc tgcaggacaa agttggtact 1200

aggaaggaat ccagatcttt tcatctcaat gttcaatctc tccaatctta aaagaatttg 1260

ggtcatcata ggatgtgatt tgtcccctgc tagaagcatg tgaaccttgt tattaatctc 1320

aatccaactg cagccagggt tttttctcaa ttgcatgctc ttcatcgtat ccctcaccct 1380

atcaacttca tcccacattc ctttagaagc atatatatta gaaagaagaa catagttccc 1440

agcatttcct ggctctaact gaaaaagttt ctctgcagct acttcaccca agcttacatt 1500

tccatgaact ctgcaagaac taagcaaagc tccccaaaca caagcatctg gctctgaagg 1560

catctcctta atcatagcat atgcctcttc tagccttcct gcacgactaa gtagactaac 1620

catgcatgca taatgctcca ttctagcatc aaccccatgc tcatgagaca tgctatgaaa 1680

atagtaccat ccttcttctg ttaggcctgc ttggctgcaa gcagatagca gacaagtgaa 1740

actgatgaaa tcaggcttct gtccattcct ctgcatcaga agaaacagat ctatggcttc 1800

cttagccctc ccatgcattg catatccacc tataattgca ttccatgaaa cgagattttt 1860

taatggcatt acatcaaaaa cattctgggc atctctgatt cttccacatt tggaatacat 1920

atcagtcaat gcactaccta caaatacatc ctcacaaaag cccctcctaa gtgaaaaaca 1980

gtggattgcc tttccatgca tcaaggctgc aatatttcca catactggta gcaagcatgg 2040

aattgtcact gaattgggct taacaccgac aacctgcatc tctctgaaaa gctccaaggc 2100

ttcgatatcc tttccatttt ccacacaaca ggcaactact gatgtccaag atataacatt 2160

cagttcaatt ccttgatttt taaactgtct aaacattctc aatgcgtcat caacaaaacc 2220

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attaaacact tccaaaatct ctgttgtgca ccggcacttc ccatacatat caataagtgc 2340

acttacaaca caggcataag atccaaatcc ttgcttaata gtgtacccat gaatctgttt 2400

tccagtcgcc aggtcctcta actccccaac tgaaggaaga acactagaaa tgctagtccc 2460

atcaggctta aacccttgca ggtgcatctt atgcaacatg gagactgcttcaaaagagta 2520

gccattatgg ttcaacccag caatcaatcc attccatgat acagaattgg gttccacccc 2580

tgactctctc atctgattaa aaagttccat tgcctcatca acatagccca tacgggcata 2640

gcccgcaacc atggcactcc agccaaccac attatgttgg gaaattctgt cgaacaccaa 2700

gcgggcatca cacatttcac cgcatttgat gtacatgtgg acaagggagg actggataaa 2760

gggatcgaaa gcaaacccac aaacagatgc aaggcagtgg agttggcgtc caattgagac 2820

tgctgacagg ccggcacagg ccttgaagac gctagggaat atgaagttat ctggcaagat 2880

gttttgggaa agcatgattg agagaagatg gagggcagag gagaagcggc catctttgct 2940

gtaggtggaa atgagggttg agaaggaacc aatgttggga atcgagtgga gggaagcatc 3000

agaagagttg aaatgagtgg catagcggga gatttgattg gtaattaggc gagtgtggtt 3060

ggataagctg gtcattgatt tttcatggtc cctaattaaa agacttgacc caacttttgt 3120

tttgattggg cacctatcct cagttggatt gatcttcgta tactgaaaac aacatgtaat 3180

gatttaccat ccctactccc caagtcgtgg aatgattgtt accaaatcag aacctgcaaa 3240

tgaaggcact gtactacacg gtggtagaga gg 3272

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<213> calyx canthus Sinocalycanthus chinensis (Cheng et S.Y. Chang)

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gaagaagaag agatgtcctc gccacctcgt tttcctcgtt tctgaacttt ttcattacag 120

caagattctg cttggagagc attttcaaga aaggtcacta tttcttaaaa tattctaacc 180

attctggcaa agtaattgtt gttgttgttg ttgggctgcg tttggatgga tggtaacttt 240

gttcacccta tgtggtggtg atagtaagag ctctagtaaa aaaggaatgg tttatcgtaa 300

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cttc 364

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<213> calyx canthus Sinocalycanthus chinensis (Cheng et S.Y. Chang)

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ggatggattt gatactcaat tttggatggg tttgggttag gtaaatgtgt caacctattt 120

ggttcaatgg ataaatgggt ttggatagag ctggaccaat tatttttaag ggggggtgtt 180

tacccattta ctaatataag taatgggttg ggggttcatg accctatccc gaattagatc 240

tgatctgatc tgaactgact tattgccatg cctagttagt tatgttatta ttattacttt 300

aattggttaa ttttttatag ttaatagaca ttctacacag ttttatggat ggaaatatgg 360

aaagggaaca tctatttcta aaaagaagta ggtttaaata ggtcatgttg gattcgcaca 420

ataaatgttt aatgggttta ttaggtcaaa ccattcattt aagtgtgggt tggattttaa 480

ggaaggggtg ggggcgtctc aatacccttg taatctttgt ttgtttgtga cttttcaaaa 540

aaaaatcttt ttttgtttgt atgagattac atattcaagc tagaccatga ttggactgaa 600

gaatgataaa attgcaccca cagatactgt tgattcttgg gtcctgtaaa accatatcta 660

gtgtcattaa aatttggcca agtaatagag ttatatggat gttcatattc tatttaaatt 720

gcttgttgtt ttcatgatct tttgatgata tcagaaagtt tggtgaaaga gcttgttcaa 780

atgtttacct tggagctaag tttgaagcgg ttgcttgtta ttgagctact ctccatcaat 840

tacaaagagt atccacaagg acaaaagcat agattacatt ggtcagatga gatctatcta 900

ggcgagtttg atgatttaag taaaagtgac atgtattcca gtgaacaatg ccagtcaact 960

cctccaagga taattggctg cgagtttgat aacactaatt ccaaacaaac tgactgtcgt 1020

catgaccgtg agattttgca ggtctattta acgacatggc tagtggaagt gaatgtcgat 1080

tggtacaggg ttgatgaaat ctttgccatg gtgggggagg aaatgcatgt cagtctttca 1140

tgagataccc ttctaagtga aatcttgaag attgcagtgt cttgtgcctt gggagtgcat 1200

gcttttattg agctatttca tacatgtgag tgcatgtgct ctcatgtttg cgatcatgag 1260

aatttaaaat atgcagcaac tgtatatttt gagatgccca aaagacttga tcaatgagtg 1320

agagattaaa gatacgtctt ttctgccttt tcaattgaac agttcacttt gtctttgttt 1380

aattgtaggc tgccaataaa tgggttgcta tatggttggg acatcatata tctaaaatac 1440

caaaaatgta ctttctctta attcaaataa tatttataaa aagggaaatg atagagacca 1500

gtgttttgat aacaagggac ttcaggcttg tctttttgtc cacttgtcat catttaagtg 1560

gtgaaaatgt tcgatggtgt ctattgatat gaaaaacacc ggtgtgacca tcatttccct 1620

tgtagaaaag gcaaaactgt attgattatc atgtaaaaga aatgatactt ttggtgtttt 1680

ggaaatatgg tatttcaata aatgaaacaa tggttttcca attgatggcc tgtcatcatc 1740

atcatcaacc attttctcat ttatttggga gcgacctttt gggtattgca tttccatttg 1800

ttctgattga agatcatatc ctaggtctat ctaggatcaa ttgctctatt aattactaaa 1860

accaaagtat gaattttact atcgtacttt tttttttcca atttcttgta gaaatgtatt 1920

tgctggtttt agttctatga ggctgtaaga tgtattttgc tttttgaatc agtttttccc 1980

agtgggggtc tgttttcagt cttctttaag actgttttga gtctgtaatg gtggagtcta 2040

gtttgggttt gttgctgtct gtatggagtt gccttgtttc ttgtattgtc cccacttttg 2100

ggtctgtaat gtaatgttga gaggtttccc c 2131

<210>5

<211>2139

<212>DNA

<213> calyx canthus Sinocalycanthus chinensis (Cheng et S.Y. Chang)

<400>5

ctttggtagc cggattggcc atgtttttgc ataataaata aataaaatca ttgctacata 60

catttccaga taaaaatcac aaataccata gcttctaatg tgggatagag atacatatga 120

caatcacccc cctcccctgt caggtttgtg gagcctgatt gtcaaatatt taatggaggg 180

caattttgta atttttgcat aacatatgtt gtagccctaa ttagggcagt atataatgtc 240

tcttttcatg agagaggagt gagagagcaa aaattgtaac aggcacggcc gtatcttctt 300

cgtctttttt caccttcgag catagtggat ctggtttgaa cgttattgtg agggcgttca 360

aggtctttcc tttctgttga tatcataaag cacacacaca tccgctgctg ttttcatctg 420

tatgacattg atcacttgct tgattgccca tccaaacagt gaaaaaacag ctggaataga 480

aagaagaaaa ccctgtttca ctgcattcac aaaaacatca atcacacttt gtgattggtc 540

cccagcaagg aggaaaagca tgatgtaaag aacctctgat gctgcacagc aataacacat 600

gaataaccga tttccataat atgcctttaa aagccaattg gtactgtctt ttacatcttt 660

gtgactagcc ttccctgcaa ggaaagtact gtacatttga aaccagtggc tggtaatatc 720

cagtgcaagc aaagacaaga aaatcaagcc aggtctgtaa atttgtgaga ggataaccaa 780

tagacaagca gtgctaaccc tgtctgttac catatccaaa actgctccaa aagtagacac 840

tgaatcagca aatgagagag ataagtcaaa tgctaaaggc taaagcaaaa ttgattttta 900

ctctgattca ctagaggatc tttgcttccc atctctgaca ttggtaccag atgttcatct 960

caccacagtg ttcaaatcaa ccaagagatt tcctccttga tttttggtta ttagtgtaga 1020

acatttcacg tatgaaggag caggtgaaaa aatggataaa aagagatgag gtttaaggtt 1080

atgaacaaag aaaatgtaga aacaaatgta agaggttaaa ttagagacta acacaaagat 1140

ttggagtata aaggtgtaaa ggagttcaac cacgaaaata tcaatactcc aagagttgat 1200

agtccacatc agtaaccaga tgctcaatcc cctgtaattt gttaaacaac caaagagatc 1260

acctccttga gttttgtttt tagtaataga cctcttcatg tatataggag tagatgaaag 1320

gaaaagaggg ggggagagag agagagattt gaggttattg acaaagaaaa tgtagaaaga 1380

aaggtgagtg gttaaattag aggtcgacac agataggact ataacgtgta tgttagtctg 1440

atacagtacc ctataattgg acatggaact tgacagcaaa aagacggatg attatgggtt 1500

tatagcagcc atcccaaggt caaggccaac acaggctaga tctgttgagt ccaagcccag 1560

acttggattg acatttgaaa ggctgaagtt ggcccattaa aatgtgggcc ccacattggt 1620

aggaggttgt gttagcacat ggatgggggg tgagtgaagt ttagccccca tccaactatc 1680

ttaggcatgt gaatttggtt cttcaaaata aatggtttgc aataaacatt tccaaagcca 1740

tgatcacatg gctgagatga ttaaataggg atgaagcttc actcataccc gcttcatgag 1800

gtaccctacc cataccttct ggacacatcg aggctaatct acagctgata gacaatctcc 1860

ttcaaagagt ccctatcact gctcaaggga agagaagcaa aaaaatggga taggaaggca 1920

taaaatgata acaatgaaga gaggcatgga taaattcaat gcagttagac acaccgtggg 1980

aggaaatcct atgcaattaa aacagtaagg caataaacca aaggtgcaga aaagaatgat 2040

agtagatgaa ttaaatcaaa tttgcagtta taggcaatga atatcatggg agatcagttc 2100

tttcatctta aatgtctaaa atatgttatc actagttaa 2139

<210>6

<211>760

<212>DNA

<213> calyx canthus Sinocalycanthus chinensis (Cheng et S.Y. Chang)

<400>6

actgaatgtc atgttaaaca ggtccattta tctataaaag aaataagaag caagaaatta 60

taacttcacc aaccatttca gtctaatcaa gctcctcttc aatttcaata aaagatgttg 120

aattccaata actacattca cccaatcctt catcgtgcca acgaaccagt acatcatctt 180

tccttaagaa tggaatgatg cagaggtatt cgaaaactcc agctatatct gatttcttct 240

atctttatcg atgaaaacag ataagaagct gaacatggta gatttcatgg gaaggtaaat 300

ttgagatggg atcacatgca aacctacaaa catctcttgc gtcaagtcac gtaaagagtt 360

gatgtcccca cttaacccac tctctctgca aagtgcaaag tgccaagtgg aaagtgcaaa 420

gagcaaatgg ttgttttctg tgacacggga aagccctcaa ttccattttt atcggggggc 480

atactcttca ttagattcca tgctaaagat gagaagtgat tgccctcaat tgtgaaaggt 540

ttgtctgcta ctctactcct actcctactc ctactctctc ttcctatgcc tgtaacaact 600

cctcattgtt gttgagttct ctgcttaaca gctcttcaac aaataattct aaagagaatc 660

ttgaggagaa ttttaaatgg taaagtcaaa tttcaagtgg atctcatagt cctactttaa 720

gctttctcat tcaaatatac ccataaacga gcccttttta 760

<210>7

<211>5539

<212>DNA

<213> calyx canthus Sinocalycanthus chinensis (Cheng et S.Y. Chang)

<400>7

gaagggttca gcgttcactg tgttctctaa gtattttata tgggagactc gtcctcctcg 60

acaagaatat aaaagtataa aacccttcca ccaaatctaa aaccaaacct ctgcactcca 120

actctctctt tcaatggctt ttcctctctg caactcccct ctgctgctct tctctttcgt 180

cgtcttcttc ttcttcttct tctttacgaa ataattgcat ttttcatgtt tccatagatt 240

cattcctctc atcttttctt tgattttgga ggatgtcaaa ttaccttttc ttcctcttcc 300

aaatgtttga ttctctgtaa tttcttctag caaaccccgt tattttacaa tccctttctt 360

cttgacctgc ttttctgcca cttcgatggt ggcctgatct cgtctcttct aatttctacg 420

gttttgaaca atatttcact cttttactgg tgaaaagggg gagcattttt accctatcag 480

gtttccgatc ggtggggctc tccaattgct tgtcaaaagg ttctgttttt tttttctgct 540

gcatttcctt ctttatgtgg aaaactattg ctttcatggc attttatttt cttaagaagg 600

tctagaacgg gtcctttttt taccctctct ctctctctct gcatcttctt ctttccaagg 660

aaaataatac agttttcact gcatatggtt ttcttgtgca cgcccacgcc caaaaattgt 720

ttttcccccc actctcattc tgcttattct tctttccgac aaagttacag ctttcaccgc 780

ttttagtttt gatagaaaac cgtgtttttt ctgcaccttt gttcttttcc atgaaaacta 840

cagtttttaa cgctctcttc gaaatggggt cctagaaact tgtatttatt tttttcctct 900

gaattttctt ctttctatgg aaacggtagc tttcttggct tttgcatttg atatgttggc 960

ctagaaagtt ccttgtctct cctagagggt agttcctact tgggtttctc gttacacctg 1020

ataagatatg gattcgtcta agaggaaaag ggtatccaca gaaaatggag atggaagtcg 1080

ggtttctgcc ttcccggatg aggttctgga gcgggttctc ggctttgtga aatcgcgaaa 1140

ggacaggagc gctgtctctc tggtttgcaa ggactggtac aatgcggatc ggtggagccg 1200

gatcgatgtg ttcatcggca actgctatgc agtttctccg gctatccttg tcctgagatt 1260

tcccaacatt cgtagtgtta cactgaaagg gaagccccgg ttctcagatt ttgaccttgt 1320

gccaccgaat tggggcgctg atattcattc ctggctcatt gcattcgcat ccgcttaccc 1380

atttcttgaa gagcttaggt tgaagagaat gactgtcaca gatgagagct tggagtttct 1440

ggcacgatca tttccgaact tccgagcatt aacgcttgtc agctgtgatg gattcagcac 1500

tgatggactt gccagtattg cagctaattg caggaacttg actaagctgg acatacagga 1560

aagtgggatt catgatcttg ggggcagctg gttgagttac ttccctgaaa gtttttcatc 1620

tttggaagta ctcaactttt caaacctgag cagtgaagtg aaatttgacg ccttggagag 1680

gcttgtggcc aggtgcaagt cactgaaggt ccttaaagtt aataagaatg ttactttgga 1740

acagttacag aggttacttg tgaaatctcc tcaattaaca gagcttggaa ctggctcttt 1800

tttgcaagag ctaactgttc ggcagtatgc agaattggaa aatgcatttg gcaactgcaa 1860

gaatctacac acactttctg gtttatggga agtcacatcc ctgtatcttc cagcactcta 1920

tccagcctgc ataaatttga cattcttgaa tttgagctat gctactcttc aaagtagtga 1980

gctagctaaa cttttgccaa gctgtccaaa tcttcgacgc ctttgggttc ttgatacagt 2040

cgaggacaaa gggctggagg ctgtgtcaac tagctgccct ttgcttgaag agttacgggt 2100

ctttccagcg gacccctttg atcaggacat tgccattgaa gggggtggtg gggtgacaga 2160

atctggcttt gttgccatct ctcaaggttg tcgcaacctc cattacgttc tgtatttctg 2220

ccgtcagatg actaatgctg cagttgagac agttgtccgc aactgtccaa acttcactca 2280

cttccgcctc tgcatcatga acccttgcca gcctgactac ctgaccaaca agcccatgga 2340

tggtgccttt ggtgctgttg tcaagacctg caccaagctc cagagactct ctgtatcggg 2400

ccttttaaca gaccagacat ttgagtatat tggaaagtat gccaagaacc ttgagatgct 2460

gtcagttgct tttgcaggca gaactgacag ggggatgcag tgtgtgttgg agggctgtcc 2520

aaagctgcgt aagctggaga taagggactg cccctttggg aatgaagctt tgctttcagg 2580

gctggagaag tatgaatcaa tgaggtcact ctggatgtca gcatgtaatg taacaataaa 2640

tggatgtaga tttcttgcga gggagatgcc gaggttaaat gtggaggtca tcaaggaaga 2700

tgagagtgat gaaactcagg ctgagaaggt ttatgtatac aggtcggtag ctggaccaag 2760

aaaagatgca ccacattttg tcctcaccct ctgagaattg gaaggccagg taaccataac 2820

aattaaaggg ttctacaccc cgttgtaata taggtgcatg taggctaatg cttcgatttt 2880

atacaatgcg gataaggctt gtgacctgta ctatcaaatc taatgtgcct cacagttaat 2940

taggggtacc caataaattt cactagttca aatattgtag ctatcgagtg aaatagcccc 3000

ctttcttttc aaatttctgc tgcaaatatc ttcttattgt caatttgtag atacaatatt 3060

atatggttgg gaccttctaa tgtgggtcat tggatgccca gcatctacct tggggtatgt 3120

gattagcctt ttggacaggc tacttttttt gaatgactgg tcttggggct tcctccttgt 3180

acactgaggc atgaggtcaa aacctacgtg tacccctatt atttggatca tctggatgca 3240

aacacttatt ggttatgaac caagtttctg gattccatgc ttctagttat gctgcagatg 3300

ttcaatgcct tgatggaagt tctgcttttc catctgcatt ttcctttaac ccaatacaac 3360

agagtgaaag agtttaggct cttcaaattc aaagaataca tgattgctgt atcagtgata 3420

tagagcatgg actacacatg cttaacagtg gattgctatt cctttccatg tgattgcttt 3480

tcgctgttga tggcttttga gaaccttgac cttatcgtga aacccagagt tgatctggag 3540

gacaatggtg gccaaatcct ttttctgtta cctgacaagt gattttaaac taataaattt 3600

ttgtcttttt tacatattca ttttgcatac aggacaatca ggcgtttgaa aatctacagt 3660

atcaggccca gttcttgtta agcagaccat cagatgaatc tttactgttg gaaaattgca 3720

aatatccgaa tatcagttag attgtacaga aaatatttac cctggtggtt taaatccatc 3780

gtttgcatgt ggtgatctcc catatcatat tagaaggaag aaaatgactt agatctatga 3840

ctcactatgt tattagtagt tgcaaggcat tggggttgtg atttgttgat tattgttcct 3900

atggtattac ttgagaatcg ggtcccactt gcctgtctac tgaagagttc cagcaagatg 3960

atgctgaagt aattgtttct tattttcaaa aagtgcaggt ttcgtggagt aaggtctaac 4020

atgggaagaa ctggagaccc agaatgtaat gaatgctggc aatctaaaag agagaagcaa 4080

aaaggaaaaa ttagctctgc ggaacatgaa agacattctc tcctttacaa agcagaaaac 4140

actgggggaa acaggggcat aaaaaaaaca acaaaacaaa gcagcagtat tttgaagaag 4200

aaaagagaaa tcaagccttt acagtgccta tggtgaatgg agctgcatca gtctcagcac 4260

ccaatgctgt acccctggag catgatcatg agactgccaa tacagaagta gatgaagcat 4320

ccatagcagt gggttacaaa tgacccaaaa tcgatcccca caatgcattg ccaccctgga 4380

ccatactccc tatcaaattc ctgcaaagaa agaagcctca gataaaatgc agttctccac 4440

aaacgtatgt attgcatcac acatactcaa attgcaagga gatatgaaaa tgaagtggga 4500

aggccatagt acccattcaa caccccctgc tgaaagtaac caatttggta attttagaga 4560

aactctgcaa tggcgacact tagccacata attaatttgt tcagtttgag agaatgattt 4620

tgtaccgtcc tctttagtag tggggaaaaa ctctcactac tttctattga gaattttctg 4680

ctactaaata gatagttggt atgatgaatt tcgtcgagtc caaatgtagc ctatgcacct 4740

tcttgatata gcatgcaatc tctatgcagt cagtgacatc gaaggaatcg agagccttgg 4800

aggcgagcct gagcgcatcc tgctgcatgt tctgcagcat gtctgtctcg ccaatgacgg 4860

ccttcccttc caacatggtg ggaagaatat gaatgaatga agagttgaaa agggggaaag 4920

aaaggtgaga aggtgagaaa cacggaagac tgcaagagta ggagatggat aagaggatta 4980

tatattgggt ttggaattag aaagtggata agtgctgttg ggcaagccac gtatatttgg 5040

aataatggat gatgtgtagc tgctggtggt tgtcaagatg ctctggaata tacaaattgg 5100

gcggtggata tggacaatat gtggggccta caaagtgggt gtgcctcaaa tcctggagat 5160

gcagcatttg gaagtaggga gggaatagct tggggtctgt tcatttcata ttgaaggtga 5220

atctaattcc acggataaag aaactattcc tatggtgtat aggttagtat ccatgcttaa 5280

catagttagt aaattccatt cacatgctaa attgcctata ggttttgttt tttctcttgg 5340

tggtcagtat tctgtccttg taggaagagg ttgatggttc tactaataaa tgataggcag 5400

gaagcagatg tagttaggaa gattttgaaa aataggaggt aagctgagaa atggaggtga 5460

tttagaggga cttgaatttt atttatccca caaggctatg gaatttggat gtggccattt 5520

cgcttatcaa tctccaaag 5539

<210>8

<211>2001

<212>DNA

<213> calyx canthus Sinocalycanthus chinensis (Cheng et S.Y. Chang)

<400>8

gaagcgcgtt actcctcaaa tacagactac agagcagagc tccagaaacc ccgcggaaag 60

ccctagaaag atatctagct gcatttttta cggcgcattt ccgacgagtg ccagtgtgga 120

atctcctcat cggtggagaa aaggaggggg atgggaagaa aatgaggatc tccgggcggc 180

ggcggcggcg gcgacgatgt cgtacacctg cctcgattgc ttctccgacc tactttgcgg 240

cgaggacgct ggaaccctcg ctgaagatgt ggaggattgt tcgccggatc ttgagttccc 300

ggccgatatc gaggattcga ttgccggatt tgtagagggg gagggagact acttgccggc 360

tttcgactat ccggcaagat tccggtcccg gtctctcgac gcctccgccc gggaggaggc 420

ggtggcttgg attctcaagg tgcatgcgta ctaccgtttc cagccgttaa cggcgtatct 480

ttccgttaac tacatggacc gcttcctctc ttctcatcgt ttgccgcaag cgaatggatg 540

gccattacag ctattgtgcg ttgcttgctt atccttggct gctaagatgg aggaaaccct 600

tgttccttct cttctcgatc tgcagattga ggggacccgg ttcatctttg aacctcgaac 660

cattagaagg atggagcttc tggtcctcac tgctttgaac tggagacttc gatcgataac 720

accctttagc ttcatcgatt tcttcgcgta taaggtcgat tcaagtggaa cctctatcag 780

gttcttgatt tcacgcgcca cccaactcat cttagccacc attcaagtga ttgattttct 840

ggaatactgg ccatcatctg tggctgcggc cgcgatacta tgcgcggctg attcaatccc 900

tgatctatct ttggctggtc ctgacgacgt ggtgtcttgg tgtgttggct tgagcaatga 960

gaggattgcg agttgctatc gattaatcca agatattgtc gtcgtcgacg gcggcggcgg 1020

cggtcgccgg aagcccccaa aaatcctgcc acagctcaga gtaacgatcc cggccacagt 1080

gggtgctgat tcatctccct cttcctcatc atcatcatct tctaacaaac ggagaaagct 1140

aaacaattgc ttgtgggcag atgatgacaa ggaaagagaa taataagaat aatagtaatg 1200

aaaaggaaat atggtgatat aagtaaaaga gttctttatt ttttcttttt cgagttgggg 1260

ttccgcagaa tcctcagtat tttttagagg gttttgagat tggattaaca aagtgagaga 1320

gaggcagatt agtatatttg ggagttggga gggttgatga tttgtttact ttcaccttct 1380

tctttattta tatgggtatt gcttatttgt taatgtcctg atgatgggga ttgcagattc 1440

ccaagggcgt ggaatttgtt aatttttttt ttccttcttt tcttttgttt gtgtgagaaa 1500

gcaagaggga gatgagatgc atttgtttgt ggattggaga gaagtgggta ttgaattcaa 1560

aggcagcatt gattgttgaa tgaaggaggg gaggagatgg tggggcaatg ttaagagttg 1620

ggaaaagaga catcatgtgg gttggtaaat aagtgaagaa gaaagaaatg ggagcgtctg 1680

ctactgtggc ggaagatagt caagcttctc actcagtcca ccatcattgc ttcaacttcc 1740

tcatttatta ttaaatgtag aaaaaaaaat atagctcttt aggctttggg gtctagggct 1800

atatgtgtgt atatgatgta cacgtgtcca aatcctgcgt gtacaggtat gtgtggggct 1860

tggagagttg ctaacttgac tggcctctct ccattccctt tggcctaatt ttggcctaac 1920

aaatactaaa ggcttttggg agcctttttg agtgctgtat ttgaaatcat taatgcaaat 1980

ttatcttctt tgataaaaaa a 2001

<210>9

<211>4320

<212>DNA

<213> calyx canthus Sinocalycanthus chinensis (Cheng et S.Y. Chang)

<400>9

gccatcatat ctgatcaaca caataagaca tgcaaatcct gtgaatatat gcatatactc 60

ggagctttac aaatattaag tcactgctta aaatgatctc aaacctagct gtcagtacaa 120

cagttagtga ggtcaaatat ggacagcacc aatcagcatc tctaaatcat atggtaaaat 180

aaggaaaaca tacaccagaa cacatttaaa gaaaattccc cacagaagaa aaacatagta 240

aatctctgca tgagcatgaa gcgtgaaatt cccaatcatc ttcaagttag cctttatgca 300

gataaaatgc cgacaaaata ttatattcac cataagagca ctgttgatga acaaaatgca 360

gagagcagta ctcaatatca gtcccagcca ctggcaacaa tagacccatg tccaagacca 420

taatcatagg aagagggatg agtgtgagaa gagggatgag cagtgccact gccaccgccg 480

gcaccataag agttatagta gtgattccct cttgaagagt tgtcccttcg gtagtcataa 540

ccaccgaatc gcccactgcc agatctgcga cttctccctc cgccattata ggagttcctc 600

tcagcatatt ggttgagcca ggacggaacc tcttgatttg attcctgcat gagttcaaca 660

agtgccttgg ccaaggactg gtttccttca ttgaaaaatg ctgttgctaa gccagtcttg 720

ccagcacgac ctgtccttcc aattcgatga acataatcgt caatgtcttt tggtaagtca 780

aaattgacta catgcgcaac atgtgggaca tccaaaccac gagcagcaac gtcagttgct 840

accataattg gagaaacacc agatttgaat gatcgcaacg cttgctcccg ctccggctgt 900

gttctgtctc catggatagc tgttgcagga aacccattca tacacaacca ctgctccaaa 960

gaatctgcac ttctctttgt ttccacaaaa actagcgtca aaactggctt cccatgggtt 1020

ccatttgctt tttgggcgtg aagaagatgc aagagatgac ttcttttctc catgtgaggg 1080

acaaaatcaa ctctctgcgc aatcaggtca gtactcgaac caacccttcc aaccgccaga 1140

aaaatgtaat ttgaaagaaa atcggaagct aatctctgta tctcattagg aaaagtggca 1200

ctgaaaagca ttgtctgcct cataccaggc tgaggcatgt ccatttgttc aacaatcttc 1260

cgtatctgtg gctcaaagcc catatccagc atcctatcag cctcatctaa agccaagtac 1320

ttgatcattt gtaaagaaac ccttgctctc tccatcaaat ccactaatcg cccaggagtg 1380

gcaactaaga tatcaactcc cctttccaac tcacgcaact gttcagaaag acatttaacc 1440

aaacatcagg aagcacatgt ccataataaa atgcaaaaat caatctccag atacatcaac 1500

agtcatctaa agcaaagggt actatagatc atacactgaa aagtgaaaaa tataggggat 1560

cgtgaaaaac aaatcaaata agatcatcaa ataagatcag agaaccaata atgagcttat 1620

ccatagaaaa ccaataatct aagcaaatca taagggggga aatttctgta aattcataaa 1680

actagaacaa gaactacaaa atatgaaaga atgaaaaaat gtgcagtcca ttaagagatc 1740

aaatctacct catcaaaact caataaatga cagtgccagt aagccactcg atttcatcat 1800

ccatttatct tttcttatca tcactgttgt cgtcgacaaa gcttgatatt tactatccgg 1860

ggctaagttt tagagaatgt ctaatccatg acagaacgca catcacaaat tttccatata 1920

cggtttcaga agaaaagaac aagcacagaa tgggctgtta tatacagaat ggatgcccat 1980

accacagatg tcatgggtgc aaacaggaac gcagcggcca tgtcaccttg aattaagaca 2040

actatgtcac aactacaaga aggtaaatta agacaacaga ctcaaggatt aaaacataaa 2100

atagacccct gcagagtatg atcattactt ctggtcaact atgcatgtgt ccaaaccatt 2160

tctacaagct tccttagttc tcttaatgga atcacccctg acctctttta caattctcac 2220

tcttagctcc accctatctt gttcatccgc atattaggct taaaatacag acatcagcca 2280

catatactat tacttctagt caattttgtg ctcgtacaac ttcatactct aatgcagtta 2340

caacaattgt gttgaaaaag tgaaccaatc gatccaactg ccccatcaac ctaagcagta 2400

catgccttca acaagagtag tttgataggt taaggcatcc caacagagac gagtcccgag 2460

aggttactac aattatcacc tgtaggggtc aattaccaaa aaagtcagta tcagtcttca 2520

ccatccccat tctattaact tgtgacccta aaattgttct tgctaccagt tttatgcatg 2580

caagccagaa ccatcttaaa tggcaataga gaccacattc aataagtcca tcttaatatg 2640

cattatgtat aaatgctttt caatcacagt ggcacacata aacagttttt gggatcaaat 2700

caaactgttg ctgcagccaa actccaacca aaaccctgtc tctccacatg cagctcacac 2760

gtatagacac aacttcgtac atactctgta atctcataac caactttaaa aacaactcaa 2820

gattgaaaac gaagtatttc gaccatctgc aagaataaat aataaataaa taaataaata 2880

aataggctca aagtgtcaca atgtgaaaca ctccaaaagc ttgtctcgat gcaacataaa 2940

ccatcgtctc ctgttcacac atggcggaaa tgcatggcat gttgatttgg tgggccagcg 3000

tgtggataag aggagatcag tgacccattt ctttttcaca caaggctgcc atcattccag 3060

taaacaatta ttaaagaaat aagtataaaa taagcacaaa acagcaaacc tgctgatgaa 3120

ttggtgcccc tccgtaagcg acagccacct tgactcccgt ctgataggag aactttttag 3180

cctcttcatg tatctggcac gaaagctccc tcgttggtga gaggatcaga gctaccggat 3240

atacagttct ggcaccgcgc tgcaaacgcg gcggccccga ccccaaccgg ttcctcatta 3300

tcccgcttat gatggggaag cagaacgctg ccgtcttccc agaacccgtc tgagcgcagg 3360

ccattaggtc ccgtccagca agcgatatag gaatagcatg ccgctggact ggggtgggct 3420

tgacatactt gcaccgccga atgttctcat tcagggcatc acccaaatca atctcagcaa 3480

atgtactgac aggaggcggc acattctcac cacttgtctc gacgggtatg tcgtcatatg 3540

catcgaatat tatcccccca gtaccaccgt cagcattctc ttcttcttcg gatatcccaa 3600

cttgggtgaa ttgatcgact acctcatcaa aagggtttgg ctcctcccgc aaacgtggag 3660

ctcgacggtt gctgcgaatg gaccgaccgg actcccgaaa actagaacgc ttcgaagaat 3720

ttgaagcaga gtttgaattc aaaggcagag gtagatccgg agaattgtca gtctggggaa 3780

taagagattg gtttcgaagg tgtggcggaa catacacaga gcgcggcagg cgggagcgag 3840

attgaggttg tggttgatct tcttcgacag aatcagacca agacgtcggc attgcgagtg 3900

gaaagagtag aaaaccaaaa ccccaagaat tcttcttttc tagaaagtag aaacctcaaa 3960

aaccctagaa atctggtaga aacctaaagc tctggaaacc cacattcgca agaaacctaa 4020

cgattcctgg aaaatgcacg ggaattcact ttccccaaat gctcaaaaca ccccgacact 4080

tttctacttc ccttccttcc ttacgaagta agctaaattt ctttaaaaaa aaaaaacaga 4140

aattcttttt ccatgggatt ccaatatcct ggaaattttc tagaagagaa attcagaaaa 4200

tcgaaaaaca ttactttttc tctttctacc cttcacccaa tttcaaaagt atttagaaaa 4260

tatcatggcg tgaaatggaa tcttatatat aggaatcttt ttctttacag gagattggaa 4320

<210>10

<211>437

<212>DNA

<213> calyx canthus Sinocalycanthus chinensis (Cheng et S.Y. Chang)

<400>10

gaattgtgtt tgaaagaaaa atggctactc tactttaaaa tgagttattg agtaaaactc 60

aacttggtta aagttttggt tgcgtcactt atttagtttt accatgttta tctcatcttt 120

ttgtttagaa caaacttgtt tatttattgg actaatttct ggtccaatca agtctttatc 180

tttgaaatct tgaggtggac tattaagcag tgggaccttt tgattccctt cccttccctt 240

cccaaattca ataaataata atgttaatga caaggaagtt atggtgtcat tttcatatga 300

aaaagagaaa agaagaaaag gttcgtgaac aaagggccca cttgaacctt agcaacatgg 360

catgtggttg aatgatggct catgaggacc cccactccgt gttgtttttt taatcttttg 420

tcattttttt ttaaatt 437

<210>11

<211>4433

<212>DNA

<213> calyx canthus Sinocalycanthus chinensis (Cheng et S.Y. Chang)

<400>11

tagaaagaag agacagaatg atctattgct tgcttaatct caaggtgaga ctaactccaa 60

aattacacag caaaaatgct actggagctt taattaaggg gttaaaacgg aaaaagttaa 120

actacaaatg taacaacatg atgaatataa ggcatggtat accacaatga tccatagctg 180

tgtatttcta cataggatca tctgaatatg ttctcaacac atcaatgtca aattccctgc 240

acaattggat cttcctctgc atgtggattc tcgagagcag taacatgctt cttgaaaaaa 300

gttgccgctt cttccctttg ttccagatat ccgagcttgt tgaagaccat agccatcaaa 360

tagaaggctt ctgcagccag ttcatgatac tctaaaattt ctagctcact agcagcttgt 420

cttaaaggat ccaacacaat ttcaggatct tcagaaacta caaaacttgg atctgataga 480

tagcattttg aaactgcaat atttgcccgt gcacgtagct ccaacccacc atggccaaga 540

atgattggaa gtgaccgatg tatgagtgat aatgcacgtt ttgcatggtt atatccgaga 600

gccagccaca attcagcaag tgtcagagtg gctgatgcct caagaagatc caggttaaat 660

gattggcaaa atgacagact tgctaacgca tatgggagac cacaaactgc attgcctgat 720

ttcttgtgga tctctgcgag taagagcaga acagtcgcat tttccacctg caggttgaat 780

ttgtaacaca tgcaaaaaag agagtgcgct actgctcctg cctggctgaa ctgattggca 840

gcaagcagtg tacgagcatg acgaacactt gcttcagttt tcagctccat atctactcct 900

gttacagagg atgccaacac tctaaattca tcacacaatt gctgcgctag tttgagatgc 960

ccccgatgta aagcacgttc atggagcatc tgcaacttta atagttgaat acgtgatttt 1020

gacacagata aaaacttctc ttctgccagc ttcagggcat tgaaggcatc tttgtaacct 1080

ttatatactg caaggtgttg aatgagtttg acataagcca atgacacatc tgctgaactt 1140

gaagcatcag caaagcaagt tacatggacc aaagcattta gtcgaacaag aggggcactt 1200

ccatacagct cccaggaagt cgctctaagc aagtatgatg caccagccag ttgtaataca 1260

gatcctggaa ttggattcgg ctgtgcacca aagtaaaatg aatcataatc acttccaaat 1320

ccactctctt gcaacaacac tgatgaagac cttgacttct gtaaattttt aagccatgca 1380

gtgctaaaag caccatcaat cgtcagcaat gccccatcag atccaaattc cctaagtaaa 1440

taggaactca gccttagttc cttggtgacg ctagttgggc atgttttcat ctttgtgggg 1500

gctttgggac caaaggatac cattggcctt ttcacatgct tgagatcaaa ttttgccaat 1560

tcaagacggt taaaggccac caagcgatta agcttcaaac tatctgccct ctttagagac 1620

cttttcaaga gtacgagtaa ttgttgttgt gtcgacaatg aagtacctaa accagtactc 1680

gccgctggtg aatgtgatga accaaccatt ccatttctgc ttggaatacc tatttcagac 1740

aagaggtcac aaatagctgc caatgtgtat gccagacaat tatcatcatt gttctgttga 1800

gatatttgaa ctgctacagc tagggcctcc aaagcttgtt tagaatgccc aaaatgacta 1860

tgcataatac ccaagcacaa taaagcagtt tgataccttc cagcaggaat caatggtgaa 1920

gaagatgaac cagtaacagt tccttccatc cctgcactgt aatcgaaata gcgatgaaga 1980

ttttccaaag cagcaggata atcttcatga taaagactat ttaggtagcg caagtaatgg 2040

acctaaagct tacacgatgt agctctggag ctagcttctg aagttgtgat agaatagctt 2100

caaatgggat caacggaaat gagagagcat gcttctcaat caaatcagcc tgctccttta 2160

agtaaccttg cacttgccag tttgtacgta ggaacaaacc tccatggtta tcatcagctt 2220

tcaatgcatc atctatagga gacaggaaat cactacctag tgtgccatca gaaccatatc 2280

tgttcttggc cttgacagaa acctggatat cttcaaccaa tccagaaagt gaacgaggtg 2340

tatgaatatg aaatgtagaa ctttctccaa cacttgttcc agtttctgat tcatgtctgt 2400

tccgctcaga ggcatagctt tcaaaatcca agtcatcgtc ctccaataac tctggttcat 2460

tattgaagtc atcttcttca ggcaactcat aggcaggatc attcgagacc tcgctacaat 2520

atgtccctat attggtcaaa aggtggcaca cgccctcaaa cgatagcaga ctgaaggcca 2580

acatgcaacg acggagaaac atccccaagt ggctgtttgg atccaaatag atctggtcat 2640

cttccatatt catttcaggg tttgcaagca cacctcgcaa attattaaag aagttaaaca 2700

aatcatcagg tgaagataaa gatgcaagac gactggtcaa attttgaccc aaccaatcat 2760

ggagtaaacc atcaactgcc ttcaattgac tagtcaattc atccagtggt ggctccaaaa 2820

aatcatcgca agacttggtg atggaaaaga ggaagagacc tagtcgattg tgctgaggta 2880

tggaagagaa tggaaaagga acggctgatt gagaaggggg agcatagatg gttatgagga 2940

agcagattga gactttgtgt ggagtgagag tgaatgaccc cactgttttc gagttccccg 3000

acatggcctc tctgcttact ctgctgcttt ttgatgcaac gggtaggact gggttaaaag 3060

gatgagacgg gtttgatgaa agggttagat ggagatggtg gattaaagga aaataattga 3120

tttaaggtat ggtgggtggc attaatgggt attaaagata agttggtaaa gggatctcgg 3180

gtggatggtg gctggatttg gttgagattg gagaaagggc agcaatggcg tgggttgttc 3240

ggggcgaaat tggagaatgg gagttgcaga tctgggacga ttgatgcagg caaccagcaa 3300

acggaatgga ggttgaagag aagaggccag gcagaggacg gcgacggcga cggcgacggc 3360

gacgagaacc agaagagcag gaacacgctg gaataacagg cgccctccga cttcagtctg 3420

gggatcagga actcatatct ggccgtgaga gttggcgacg tgaatcgacg acggcgttga 3480

ttcagatctg taatagagga ggaagcagga gtaggccggc gagcagaggg ggaggaagaa 3540

gagagattgg caagggatcg ggagaggcga cgctagggaa ggaaagagta aagggaaggg 3600

acgtgtctgg cggtttgatt gcagagaaag gggaaagcac ggggaaagcc aaaagggata 3660

actcttatta attctttctt gtaagaccac ccatctctaa tttataataa aacttaaatc 3720

taaaaataga aaataaatct aaaacgaaaa tagaaaaaat aaaaatagaa ataaccccta 3780

ataaatctaa tattacaaat aagacaataa aaataaataa aaaatagaaa cttcccggat 3840

ctcttcaatt ctttggccgg atttccaatt cgagcgagat tctgattttc gggtcgatac 3900

ccgtgctaca tcagtctccc ctggttgaaa aaaaaaaact cgtcatcgag ttcggatcca 3960

atgtgagaat cgccgatact tgatcacaca tttcttgaac ttcgacggtt cgatcacgca 4020

aataatatga tctggacatt catgatgaac cttgacgttt gtgatttcaa taggcgtgga 4080

ttgcgtcgac tggaacgcca gaaattcaac tgattggctc tccaacactg gtactgacag 4140

tggtgtttgc acaggttgca actgtagaaa ggatactgtc tctcggagaa ctggcatgca 4200

tcccgaggta gtagacacta attttggaag agcgggagca agttctgaag ttgtctcttg 4260

ggaaagaggc acgattcccg atgtcaactc tgattcaacc aatgtcggga cgttgtctgt 4320

gtctagtggt tcaagtgaga ggaccgggac cgatgcaagg tccgggacag tcccctgttc 4380

gagagacact acgacgtcgc ttgcttcttt aaacttttgc tcattagcag caa 4433

<210>12

<211>763

<212>DNA

<213> calyx canthus Sinocalycanthus chinensis (Cheng et S.Y. Chang)

<400>12

ctctagatca acaaaatcca ttcatcttcg agttcaagtt ttcaaaattc aagtttgaga 60

ggaggagaaa ggtttggatc tagagatggg gaggagaaaa atcaacatcg cgaagatcga 120

agatcgccag aagcgacatg tatctttcag caagaggcgg atggggcttt tcaagaaagc 180

ctcagagctt tcaaccctat gtgatgccca agttgctatt gttgtctact ctgcgtcggg 240

aaaaccctac atctgtagca gccctagcag tgccgaaact gtctttcacc gttacctcgc 300

cacagatgat tcttcttctt cttccaccaa caaaccccag tcgtcggatt ctgccatttc 360

caatctcgtc ttgctttcca acctcgccac ccaaattgaa gatgatgagg gtcatgtcgt 420

tcccgaggag aagaggactc aattggttga gactgggaag gggaagggga tcaatgttga 480

agccatcgac gattttgatg agttggagca actgcagaaa cttttgaata agaagaggga 540

gatgttgctg gcccaggcta aacaatcgct agatgaacca tctgcgtctg gtgcagattc 600

tgatgaattt gatattgatg acgaagaaac gatggagaga tctgctgtac cattgcttcc 660

atggtcctac aacaaagagg atccattgta ggcgatggcc attcactgct ggtatgggca 720

gattttaggg ttagctaggg gggccataag gccttatggc ccc 763

<210>13

<211>3855

<212>DNA

<213> calyx canthus Sinocalycanthus chinensis (Cheng et S.Y. Chang)

<400>13

ggaacggtca tagtatttaa aagtaagaca aagaaggaag atctagtgaa actctatcct 60

gtgaagaagt tcattatgca tggagctaca ttgttgtcat tgtcccacat atcaagtgga 120

tcatcaaact aaactcatct atttgaagtt ggctttctgt aagatatttt ttccactcaa 180

tcctgtcaac tctattttac atcattccac aaccaagagc ttctcctaat caaatcagca 240

gctatgtttc ttgaataaat gaattttggg aagtgttttt agccatccaa taatcccttc 300

taattgccct aaaaatggaa tcatctaatt tcctaatcaa tacatttaca tgatggacca 360

acccacctat tggatggctt agatgttgta tgtgcgtgcc acgtccgcat gtcgagtcaa 420

gcatatcgag ctgcacaagt aatctctgac tgtgtgctca tctccctggc accaacatca 480

gaagcttatt tatgcaagtg caaagaagcc atttttgccg atgctacttt cctccaaaaa 540

atattacaag tagagcaata actagagcat gatcagttcc aggatatatg gtcagccgaa 600

acttgctcct gccaacaatg actttcccaa gcatgtgcat ggcactagtc tgtgcaatta 660

tggaattccc cttgtaaatg atacatgatc tgtggaaagg actaccttgc accctaaaat 720

ctgatgttga ctcttcccag ttttcaccta cgagaaacac ttgtagctct gttctcaacc 780

aggaatgcaa ggtcctcacc actcgaaaaa tcgaatcttt caagtcacaa ctctcccctc 840

tataaccttt ccatccatca ccctgaaaga agcatcatac aagtaaaaat aaggatatca 900

tagttttgtt tttgttttta aggagacatc atagaataga atgatttgct aaagatcagg 960

tggaataaga aacagtattt tccattttat atggatcaca aggagtgaat ctactccact 1020

aatggcacag ttaaagccat tcctgccact tatttaaggt atatatggta ttatttgata 1080

acctgaacca tcaaaagttg tagttgtgct gatgacagaa cagtttaaat cttatctagt 1140

gccacatgca atttttgaca tgggagaaag tagcagcatc aatttagcag taggttcact 1200

aatgtagcgg aagcgggaga aaatcgaaat aagtcaattc cggctcgaaa tcgggactca 1260

cacccacgta cgttgttcac acgtgtgaag tggacttaag tcacacacgt gggaagttgc 1320

tgaagttagg gttgcgaatg ggttttaaac aggaaaaggg gaaagtgggt ttcgacagaa 1380

aggaaagacg aaagggctag ggagcggcgg aaaagggaga ggttgcggca atggaaacgg 1440

aagcttggag acgtaaggga agggatcgga gggaaaagga gatgaagatg gaaagagacg 1500

atggttgttc ctgggttaag gtgaggatgg cggtgaaggt atcactggga taaagccaag 1560

gtatttgttg tagcagtgac agaaaacctt cctcttggga tgaagcccaa gagtcgtggc 1620

cggagtaaac cagcagtagg ggaagacctg caacacggca gaggtgcacg gtaagagtaa 1680

actctctttg attgaacaaa tattttcgga aaatagggag tgttcttttt cttctccctt 1740

cgtttattat ttataatcaa atctatggcc gactattaca atgattcaaa gggtatttcg 1800

gccgactatc ctagaccctc ttttggaggt ttcctactta cacacaactt ctaatcctta 1860

tcaaagactc cttctataac aatctaacta taacaacgac tcttttactg atccctaagc 1920

caaaatacga aaattaaaaa ttaaataaac taaatgaata aggcttaatt cgtgatcaaa 1980

cttgcgggcc acgcttcttg ttgacccggc gtgcatattt cttcaaaaat ctgtcggcca 2040

catgttctac atcagaaggt gtggtggaga aggctgcaac aggtgcggtg cctgctctac 2100

aggggcagta gagaggaaca agagaatcaa acgaatggga atccacccga agagagagaa 2160

gagaagagaa gagaagtgaa gagaagagga ggttggcttt ggccatggca gcagcctctg 2220

cgagggctct tctcatctct cgcttcaatg acttccgtct ttccctcaaa ttccctcctc 2280

ctcatcacct taatctccac cgtccatctc atcggactca atcccagaca cccgccaaat 2340

ccctctcccc atcggttctc tgctgtctcg tctccggagt cgatggtggc ggtgttgccg 2400

acgatttcgt gcccacccag aaatccgccc ttcgccgaga attttctgtt cttgccactg 2460

tgctccggca gattgagcca ctcgacacgt ccatcattgc agagggtgtt tctggcgccg 2520

ccaagaactc catgaagcgg accatctcca ccatgcttgg cctcctcccc tctgatctct 2580

tccgtgttac catcagggtt accaaccttc ccctccatcg cctccttgtc tcttccatca 2640

tcaccgggta tacactttgg aatgcagagt accgggtttc tttgatgaga aactttggtg 2700

cttcttctgg ggagacgagg acaaatgaag tgtcatcaaa tctgtccagt gagtgtgaga 2760

caccaagcga tgagtgtgag gacagagtag gaggagatgg ggtggatggt tgttattcta 2820

gcaaaagtag tgagggcaat ttggttcaaa tgcttggtga tttacctcct gctgccttga 2880

attacataca agagctgaaa tcgcaattgg cagatgttga ggaggagctt aatactcaga 2940

agcaggaaaa ttatcatatg gaatcaaaaa aagaagagga caatgatcta ctagattact 3000

tgagatcctt ggaacctgat atggtaactg agttaagtca accgtcgtca tcagaggtgg 3060

aggaaattat tcaactactt gttcaaaaca tattgcagaa attctttaag gatgacactg 3120

ctagtttctt tgaagattca gctataggaa agacagacag ttgcactgat gataatgccg 3180

agcattgcga tgctattgaa acatctcgag attatcttgc aaaactactt ttctggtgta 3240

tgctattggg tcatcacatg agaggcttgg agtacagatt gcacctaagc tgcatagttg 3300

gtatactgta gaggggaaaa tagaaatgat gtatattatg gatcagcttc aggaaactga 3360

tttatatggg tttgtatcct ctttttctat gtattattta tcttttattt gttaaaagga 3420

gttttccttg ttaatggtgg taattttata ggatcattaa atggagtttt gtccaacatc 3480

ttgtttcttt gtgatgataa ttatgaggcg gttaaattgt gccagcatcc acaacttgtc 3540

tgactctgct tcaggcattt cttagttctc actgcccttc ctaagatcat ccctttgtgc 3600

tgtgctttta atcctgttct cattaatgct tttttttttt ttttgtgcac agaaggatca 3660

ttttccagtg tattttaggg gattttgatg agattccttg ttgttccttt tcggaagtta 3720

ctttatgttt ggagaatgac ggcaagaaat taaggaattg ctttgtgcag aactattttc 3780

ccccatcccc caatctaatg ttttggttgc tttgctgagt gtaagacata gtcactttgg 3840

ccgctttgag aagtg 3855

<210>14

<211>3259

<212>DNA

<213> calyx canthus Sinocalycanthus chinensis (Cheng et S.Y. Chang)

<400>14

cctagacaag gtgtcaatta cggatgtacc catgaataac agaagggtac tattcgaaag 60

atctggaagg tacatcagct gccaaagatt ctgctaatgt attagagaaa aacaagaatc 120

aaagaaagtg gcctggacct ctcctcattt caccaacttt aacacagaga actcttttat 180

tcaattcctc ccattccaaa catagaaatc agctgtacaa ttaaaaaata acttggagcc 240

attcaacttc tgcgttggat gcttcgatgc ttcactaaca cagggatgaa tgcaaagttg 300

cttgttcact aagctcgcaa agatgacccg gccaaccctc gattaagcag tcttttctag 360

ccacccaagt caataaatca tgctcacatt ctacccaata tgatagctag ttatctattg 420

tcaaaacagg tgattcagtt tctgtcctac caatcatttg ccatgcagta accaatagga 480

agtgtgatat ggatataaat taagccttcg gttagtgccc atttgtttcc tttgacaaac 540

atgagtgatc agcttccctg acaaaacgag atttggctac ttctggagat gagaatgaac 600

tccatgtaaa cattttggct tccagctgat tgttcaggaa ctcattggcc aaaatggact 660

gcaagatgca cttaattcca ggtctaagtc atcccccacc accaccaccc tcaaagccat 720

aaccatccat cacagctgag gctttgtacg ttttactttc tagtcatctc tgtttggagc 780

tactcttaaa ccttcaccac atgccatttc tattgtccca agctttcttt caaaaagtgt 840

tcacagagag agagagaggg ggttgcaacg ccagcacttc ctagttgtca acacatccta 900

gtaccatttc aagtattctc agctgcggta cttattggat ctgatggagg actactggta 960

cagtgacatg taactattgt ttcaagtgtc tcagaatgaa tatataatca gtatattcca 1020

acttttcatt gtacagcagt atctgcaagc actgaataat atcagcagtt gatgcatgct 1080

ggcaatcggt acataaagcc tacctgtagg tggttcacta aaacagatgt atctatacaa 1140

acatgaaagg ggtagttaca atgtctggaa acgcttcatt gaactgatgc ttaggttaga 1200

ttgagaacct ttaacagcat atacaattgt gaacatcagt attagcatag ataactttgc 1260

atttagtgga caatagacaa aagaagccaa agatggaagc acagaccagg acactgttct 1320

gagaaatgct cgtcagcagc agaatcttca gtatcctaaa aactggtttg atccattgac 1380

atttagatca ccgtttcgga caccctctaa tccgtcaaca ttctgacacc ttgatacatg 1440

gcaccaggac ggaggtcatc catagtatct acatttctga agccacttaa ctctctcaga 1500

ccaagccacc cacgtcgcga gaggaaatca cggaagtcat cttcagtgta cagcgtcttt 1560

tcttcggtat ggacaggaat gcggtcagat tcatcataga ggcaaacttt tattgtcagc 1620

ccctcatcga gatgaagggt gtaggtaccc aacggcatgt ccctgtcaag gctgcgaaca 1680

actgcatctt catcctcaag ccaaaatgcc cttttcgttc tcagtccaaa ggctgattta 1740

attgcctcct tgattgcatc tgcagtgcca tcgatgccaa tccttctggt gtactcaccc 1800

cacttgacta aaataaccct tcccccataa gaagaatgat catctccacc atttgcaggg 1860

gtgtctctcc aattccatgg tggaacgccg ttggcagcat cagctgcagt gatggcaaga 1920

ggatgtccat catgatccaa acgccgttcc aaattgagag ttgatctatt gccttctaca 1980

gttccatagg aaatgccagc ctcttcaaga cctttatctg agaattgcag gtaggaatca 2040

accttggatg gggtggggct cttataagct gaagtcttag tcctctccct gattaattcc 2100

tcgagttcct tgtaatatga catctttgcc gatccactcc ctctatcttg attcctaacc 2160

ttcttgtact ccttcaacag attcctccat ttatcagtgc acattgttgg agaccgatca 2220

aaccccttct ctctcatctt tgacgaaatc tgctcccaga gatgcttgtt tgactttgat 2280

gtattgaaaa gcccgtccat ttctcgccgg agactgatga ggcttcgtgt ctcctcctgc 2340

acccacgtct cggcacgttt ctttggcgcc ttcatctcat ggtcttctcc actgctctca 2400

gcaagtatca tctgctgctg ctgttggggc tgcaagctac cattgtccac aacttccatc 2460

atcatctctc ttcccccctc cttgtagaaa tcaattggtt gcggcttcac agataagtac 2520

atatcgtgcc ctcccttctt ctctcaaaca cttggccaaa aggaatatga gttattctag 2580

ggggtgaagg aaacggaaac gggggaaaag gcaaataggg ttttgaggag aataaagaga 2640

gaaagagaac ccatgtcttg gattgaaatt cagcagactg gaaatagaag aaaagggtaa 2700

cgagtgtagg agagaaaccg ttctagctcg agaagggaat ggagatacgc catccgaaga 2760

catcgatttc aaagagcata aagtgagatc gaaaacccat ttcctagatt ttgaaattga 2820

gttagtttag aaatggaaga aaaagggcaa cgcctgaggt agagaaacac gttatggctt 2880

gagatgggaa cagagataga caaaagaagg acatggtgtt gtaaaaggag agatggagaa 2940

caacccaagt cttgaattag gaaattgatg caagaatgga aaggagaagc tgttctagtt 3000

agaaaatgaa agagaaaaat gcaaaagaaa gaaaagggat ttgtgggaaa agcaaacccg 3060

aggaattttg agatagaaga agacaaagaa agaagcaacg gcagaaagaa cttcttcttc 3120

gatttttgga ggggacgtaa gaggaaagaa aggctgattt gcaggaaatc gaagataaac 3180

ggaggagaga agaagaaggg aagggaagta gaagcgaagt ggaccctttt gctgtattgt 3240

gtcacccagt ggcagattt 3259

<210>15

<211>4605

<212>DNA

<213> calyx canthus Sinocalycanthus chinensis (Cheng et S.Y. Chang)

<400>15

agagaaaaaa agggccaaga aaaagaaaaa aagaagaaga gaaaacaata gaaaaaaaag 60

gaaaagaaaa aacaagtggc cgaaaaaaaa aggagagaaa aaattcaaaa aaaaagtcac 120

aaaaaaataa attcatggct ttttactctt acggctcgag caaagctgat aaggatgaat 180

tctacatgcg gcgtacatgt agacgtgcaa atcaaggcta ccgtagcaag gtagaattgc 240

ctcctttcaa tggtcaattg tacgacgaag atttcattga ctggctttta gaagttgaaa 300

tctttttcga tttcatggaa atcgacgagg cgaagaaagt gaagcttgtt gcttaccgat 360

tcaatggtta tgcttctgct tggtgggaac agctacaagc cacctgttac tgacaaggca 420

agcttcctat caagtcatga ccacgaatga aaatgttgct acggactcat ttccttgatg 480

ccgactatga gcaattcttg tttgagcaat atcagacttg tcggcaagta gatcgcacgg 540

ttcgtgacta tgcagctgag ttctaccatc tctccgcgat aaacgtctta gtcgagactg 600

agtctcaaca agtcttcaga ttcatcgtag gactccgtac tacaattttt gcctgcattg 660

aattccactc tatcttcact ttgagagatg cagtacgaat gaccagaaga gcagatggtc 720

aattagagct actcaaggaa aaaaatgctc ctatgactgt tgctaatgaa ccagagtcaa 780

tagagacaag cgaagccttt gtgtctattg aacaagagat agtcccgaac attgcattgg 840

tcccggatct cgcacctgaa ctatcacaga caaacaagat acaaacattg gtcgaatcga 900

agttgacctc gggaatcgtg catgatcaat caaagtttgt gcctatgcct ctagagtcaa 960

ccttggaatg tgtgtcagtt tcctcagaat ttattccatt ccactaaaga cattctcatc 1020

tccgcacttg caatcagtgc aagcactaca gtcggtgcta gtgcataatg tggagtcaac 1080

tcacctacca ttggtgttcc aactaccaca cacacgtgga gtactactca acatgttcaa 1140

gcctttgaag cgcaaaatag caaccatcaa atatcgaagg ttttcccttt ggttacgaac 1200

tcgaggacga gttcctttca accatagggg actgatgtag ggagcttcct ctttaaattt 1260

tcttggatct atcatgcgtt tgaaattaaa tttttgaaat caaatagcat gttaagatcg 1320

tttgaaatta agatgtaata ggatggaagt tgttttttaa atcaaaattt gaaaatcaga 1380

tttgatttca aattttgata attagatagt atttttattt tatctttatt ttatttcttt 1440

gcattttcct cttgtattaa tacccccttg ggctgtaaat gaaataggac aagagtatta 1500

caatgaattg aaactcagtt ttgtttgggt tctacccatt tctatcttct cgtggattcg 1560

agatctttcc tgtccgacgg tgatcgaacg aagttatccc ttcctccttg ggtacctgct 1620

accaatcacg tttggagcat cccgggagtc tcaccggtcc tttctttgga agaaggacga 1680

cactttctcg gatcagcaaa gtgccatcga ccaaggcttg gctattgttg ctgctgccgt 1740

gactcttctg accacaacct gtcagcccct ttgctgctgt ctaaacacca ttcctgcctg 1800

agaggactgc cctctctctg ttccatgtat tccctccaat cattaatcca cttttacccg 1860

atccccgctg cgtcagaagg gaatggaaaa aggaatgggt cggaacaagg gttggcttac 1920

gtgttttagc tggaggatgt tactagagaa gaataagaat aggagaaaga ttagacgtgc 1980

cctgaggagc attatggtat ggtgcaaaaa gaataagaag aagaagagat tgaaatagga 2040

ccttctaaaa tcattgaggg gattagaaca aagaggtatt atttggtggg aagggtatta 2100

gagaacaagg ggtggcttgt gtgtattggc tggaggaagc taggaagtga atgatgagag 2160

gcgttcaaac gaagtcaaaa taccctaact taaagaagta cgagtaatat aaaactaggt 2220

gggagcttaa ttacctaatg cctgctttag gttgggtgag gcaaataaga tcttgacatt 2280

tcaagcacac caactatgcc tttgactaat attctagatg atcctctcca tggatgttga 2340

atttggaacc ccatgtctca aactctattt ttggtagatg gaagtacatc atcatcaggg 2400

ccatttattc cttctccctc tttttttttt taaagaaaaa atttgttcct tctatttggg 2460

cgtgtcactt gctacttgta aatagatttg gcaagccttt tcttctgact gacttagttg 2520

cctatgtgat gctcatagac aagaacttgc attcttatga ttgcaacgat gagcacttaa 2580

tcaactgtct tacaagctct taccctttag atgggagtac atacaaattg tgtatgtgat 2640

caaactaccc ttgcaagtgg acagtgaatg aggagctagt ccagtgacta ctatttctag 2700

catagaatgc cagggaatgt tccatagtac ctatctgacc tttttgtttt cacgagttcg 2760

tttttctttc taattactac taggtagctg agaatttgtt tagaagctat cctataattt 2820

ttgctgggaa agtagcaatc tctagccact aaagtctctg tgatactgtg acgttcatat 2880

tagaaataaa atgataatcg tcgacaacca tgttccaaaa catacacctt ttttgtgtgt 2940

gtgtgtggat tagggacatt tttctattct gcattggata ttctggaaag ttccttgttt 3000

ggtgattctg attgtggaag gctttaatag aaacttctta cggggacaag tttccttatt 3060

tatcattact tgatatttct tgtcgagtgg gatgtactta atgttcccat ggacctgaat 3120

aacatttggt cttgtttatg cagattatta aactgaaaag agaccctaaa gaatacatgg 3180

attagtaaag attaaggaga aaagagacat acccaaaagt gactgactac ttgttacttt 3240

gaaagctatt tgagaagtat tcactagtga tcatcttcat caacttttaa atgtacaaat 3300

gacttgtatg ttccagtttt tggacttttg gcttgtaacg ttccccacta actggaactg 3360

tcaaaaccat cttttcatgg tttggaaatt gttctgcatg ttttcattta catttgtagt 3420

ttgatacctt ggttttgcat actatccagt caacaaagaa tctagagcat atagttgcca 3480

aaagagaaag gatccttttc tccagagtat gggagcccat caaagtcctt acagctaaag 3540

taaagagaat cttctacatg gggttactat tgttgaatgt aaagataaaa tcgagtttaa 3600

gaaagatggg gcaaagagca aagtgattgg accaaagttc ataattttgt tcagaaatct 3660

gtgaaactgg tgcaggtaaa agtcatgatt ttgacgttta gattctagtc agaagatatt 3720

tggggatgtt aaaagcatgt ttctcccttt ggcacgtgaa accaatttga tttatcacat 3780

ttatatcttc tatcatttca atgacacgat aattgttctg ttagtttgaa ctcggttcta 3840

ttcaaacatc tacactgttc tgatggtaga tgggttaaga cttaaactat aatgatatat 3900

tactatgctg aagctataaa atggttgtca gtctggccag gagatggtag ggcataactt 3960

tgtcttgatc aaggttgcat caacatctta cttgaatccg atgcaccatc ctcttattct 4020

ttacatcttc atggggtgat atttatataa acgttgtgta ttcacatgtc gttgtaaatg 4080

cagagacagg tacaaaagtt cacgggatga tcatgactca cttcggaggt ctatctccat 4140

ctcaccctct ccacgtagca gaagggatta caggtcacct caaaggtctt tgagtccgag 4200

ggagaattat catagtccct cgaggtctcg ttcctacagc cgctgatgag agaatatgtt 4260

cgaagaatgc tggctccagt cagtcatgat ggtatcggta ttattgatag gttttcagta 4320

tgttgaccag cctggtgaat ttaagttgaa gggagtttga atctgcagtt tctggtatgt 4380

ggactcgtag atcaagttaa gatggtggct tgctcttgga tttatgggtt ttgatatgcc 4440

tctcttgcca gcttcttgaa aagaacttgt agttgtaatt atcgactgta gtgtgagact 4500

ctttccatat ttacgaagtt atctattggg tttatactat gaaaagttaa acttgaaact 4560

tcaggtgagt ctagcagtta aaatttcagt ggatgcccct tatac 4605

<210>16

<211>1507

<212>DNA

<213> calyx canthus Sinocalycanthus chinensis (Cheng et S.Y. Chang)

<400>16

aagctaaact gaaatcactt gaggtatgtt tctccaaatt tcttttgtca gcatttgtgc 60

attatgtata gtcattttat acaaaatgtg tttttgaatt gaatgagtaa atgtatttac 120

caaaatattt aagggataaa cgtatgtggc catgcgccaa cacactccta tagacaaata 180

aattgtgtat tgtaatccta aatgttctaa tacgtgtgat gaatgcatat tagacttgtt 240

actagcagga aagaattcta tatgtattcc gtatacgtgc acctacttta ttctatagga 300

cagattagta acaaaacaat gaaaatagaa tcagacacgc ttgcatgaac attccaaaga 360

aatttcagtt caaaaggcag cccaaaattt tcattcatta tatgccctat gtacagatgc 420

cccaacagta cagtggtgat cccagctgat gtttggcctg tttcttccca ttgaatttct 480

cctgttgttt ggaccttttc tttttaagat gccctcatat aattcccatt agttcagttt 540

ctctattgaa taataaggaa attataaata aaaggaaaga aaagccaata taatctttgg 600

agtatcctat tcaaagtagc tcaagcattt gtcatctaca tcaacattgt agatggttcc 660

aactaataac aaataaagtt tcctctctct ctctcataga ctataaaaaa aaaaagaaga 720

atacagaatt ttgaatcgca tgaccaactc tcgtcaggat gctttgcaac ttgtcacacc 780

tctctaagtt taaggaactg attacaaagt tcttaatcac atgactaact cttttgaggt 840

tacttgacaa cgggatctgc tgcttttcta gttttgcatt taagctttga atctatggca 900

atgataacaa agatccttct aacacctgca aaagatgaga acaacttaat gtgccaactt 960

attgaacagg aacaattgga aaagaaaaag gctgagtatg cagagaaaat gaagaatcag 1020

attgctatca tccacaagga agcagaagag aagagggcaa tggttgcagc gaagcgtggc 1080

gaggagattc tcaaggctga ggagatggct gctaagtatc gtgccaccgg gcttgcccca 1140

aagaagctcc ttggatgttt tggtgcttgaagttatcaga catgcacaag gggggcttga 1200

aaaagaagaa aattaaaaac tgataaaaaa aaggaaggaa aaaagtgtgt tttttctgta 1260

attttcttat tgtggttgct gtttgcaagg gtttagtgtt ttaggtaggc ttctgctgtt 1320

ttatgtgtat agtgtgatct gtttattgtt tatatttttt tatatctgca tataagattg 1380

atgacaatta agtttcaaaa tattctttag tttgaattat tagattctta tgtttatctt 1440

gtagctattt ctgatctggt aaatattaga caaagagtgc agttaattga aaaacaagtt 1500

tgtcaaa 1507

<210>17

<211>958

<212>DNA

<213> calyx canthus Sinocalycanthus chinensis (Cheng et S.Y. Chang)

<400>17

cttccgatca cagaagcatc aagaaatctg aaggttggac ttactgcact caagaggaaa 60

tgcagagaat ttggtattcc tcgctggcca cacaggaaga tgaaatctct tgacactctc 120

atacatgatc ttcaggaaga ggcagtgcgg caggagaggg agaacaaagc agctgccatg 180

gcagtgacaa agaggcaaag aatcctggag agtgaaaagg caagcattaa gaggaagccc 240

ttcatggaca tacaaagcga aacaaagaga ttcaggcagg atgttttcaa gcgaagacat 300

agagcgaaag ctctgaacgg tagcagcagc agcagcttct gcgacaacat gtacccatct 360

gaagaaacag catcactaaa catatgatta agaaataaat acaaataaca atgttaagaa 420

atcttgtgac agaaataact cagcaattca caacatgaag gtgatgtcaa caacaacaaa 480

accaaaattg actctgtttg ttttctggtt gactgagtgc tatttttaag agctgcaatc 540

ctaattccat tcctccagaa gaactctcga gtttatgaag ttttttactc aatgtggtga 600

cagcagcagt ggtgttaatg ttaatgatgg tggcagctgt gatgaagact gtgatccttg 660

taattataga aattattagg gactagagat ttttgaaatt ggctgttact ggatcatcca 720

caattttgtg ggtgaattat gctgtgtaat gtggatatat atatatatat atataagtat 780

acattagcta gttgaacaat attattatct caaaactttc ttcctttgga gttaagatga 840

aaataccatt gaataacgct ggggactggt ttttacaagg tctttcactt ccagagttgc 900

acaagaggaa tgtggcttta aatgaagcac ataagatctt tgttcacctg tcgcatat 958

<210>18

<211>1672

<212>DNA

<213> calyx canthus Sinocalycanthus chinensis (Cheng et S.Y. Chang)

<400>18

gaacgaatgc agcaggaaaa agaagaaaga gattaattta ttgatcaacc attcaactac 60

attctctaat aacagggaag caaagttcaa actctcttcc catcaacata ttttctttcc 120

ttctctctta ttccaaactt aagctgtcac agatgtctcg atcgcaacct tgcccgagta 180

cctaacgcct tcagacttgt cggcgtcttc ccagagctcc ggcaacgctt ctctcatgtt 240

gtggatgctt tctagtgcaa agtcagcacc tttagttctt tgagaagttc caactagcac 300

tgtgtggaga ccgacgcgct tccctgattg gatgttacga acactgtcct cgaagaaaat 360

tgttttttga gggttgatgt ttgcaattct catagcttct tcaattgcac tttcagatgg 420

tttgcagaga actggtgtct taggaagggc cgtgccggca ttcggtcgag aaaaatgctc 480

aagaatgtca aaaatacgag agccttcaat cttgaatttt tcatcatcat catcatcatc 540

atcaacaaca taagtcttgt tgggattcaa tgtctcaaag caaataattc cttcaaaaca 600

atcttccagc ccgagtcggc ttagggcttt agcagcatga atcttatccg catttgtaaa 660

tataagtttt cgaagaggca gactcattag gaggcttctc agaatcgggt ctggttttag 720

cttttcataa ggtaaccttc cgtgaacaaa actatggtaa tcgtcgtagt caaattcata 780

cccaactgcc cttaggccag ccattgttgt cccatagttc ttgtagagta gattgcacat 840

atcagggatc ttgctctcat ctattccaag cttctcgacc atatagtctc caatgttctt 900

gcggcattct gtcgccaaac cagaactgag gggatagaga gtgtcatcta gatcaaaaag 960

aaggcaatca tacttaggtc tgtgtgtttg gtgatatcca tattccatct cgatttaaga 1020

agcagatttg gcagaaacag agagagagag actatgggag aattggagac gaggatgggg 1080

aggaggagaa gagtcgggta gagcggttta aatagaaaag gattgcaagt gttaaacgtg 1140

attagagatg aagattactt tattagtgtt tgattggagt ttttaaagga atttagtggt 1200

tctgaacttc tgatgtaaaa ttagagagag cttcccatat cgaaagcatt agattcctga 1260

attcggacat gatccacagt acaggcagta gctttttcaa tcagaaccgt agatcctttt 1320

gtcgccgaca acataaacat cacgaattag aatcattttg tcgcaagcaa ttttgaataa 1380

acgggggaag cgctctttca agagtctatc acctaaccat aagtcctctc aaaagaaagt 1440

gtgatcaccc ttaaccacta gaaatctaag gcgctccttg aaaaacttat caacttcagt 1500

cctcgcctga cacacctttc accaaacttt aagcatccta gaactcacct tgtccacctc 1560

cttctccaaa gaccacttgt tcaaatcgga agtaccatac ttggcacaca aaacacgaat 1620

ccgtaagaca ttaagttcac gaccgaaacg ccaactccat ttacataata ag 1672

<210>19

<211>3046

<212>DNA

<213> calyx canthus Sinocalycanthus chinensis (Cheng et S.Y. Chang)

<400>19

catcaacaaa tacgctatac caaaatccgt gcgtaggtgc gctagcgatt gcaactcttg 60

cgcgcttcct cacctcaagc ctctagcttt agtgggaaat ccttcacttc ggcccttcaa 120

gctcctcttc ttcttgtttt ctccttcttc ttctttgttt ctttgcttcc tagggttcta 180

agtcttagag tggaagatag agtggagatg gagaaaaggt gaaaatgaaa tgcaagagag 240

caagggtcgg ctatgcatgg ttcttctcct tgggttttat ttcaaatgca accctaggcc 300

aaccactttt acctcatttg tttagaccat ttcctcccaa ccccaaaaga tcttttctct 360

ctttcatcca ttcagccatg atgaggtcgg cattgcatca taaagcttgg gttccattta 420

aaatcttttc tccattttgg gttatgtttc tcccaccctt tgaacctact tttctactag 480

cacaagaaag gcaaaaagcc tttgcttttc caagattcaa tcacaacctt tggtttaaag 540

caagatctaa tcttagccat ggattttaaa tttgatcaaa tcctagccac tagttcaagc 600

tagattaaat cctatccact ccctcatctc tttgttttcc ctccttaagt ccaaacccta 660

gaatttaaat ttgagtttgg ttgaccatgg gtcaacccat gcttaggtgg atgccatgtc 720

atcaatccac taagtccatg tggggtccac attgccactt agtaatgcca catcatccaa 780

tttattaatt aattagtaaa ttgctaatcg gagaaataat tcattaaaca agctactaat 840

tttatttatg ctaagtaatt tattctccaa taccatgacc aaatttaatt taatatttta 900

atcatattgt cactttcaat ttatttgata atataatcca tcataaacaa tttaattcac 960

ccaatataat attactttaa tcacttatga cccattgggt caaaattggt caattggcca 1020

aattgaccca tccaattcca tttccaactt agactttaaa tccagtgcat ccataattga 1080

ataatataat catcaaagtg gggtccaacc cttgatttaa ttagcggata aaatttcggg 1140

gtgttacatt tttcatctaa atttatcaac ttattttatt taaaaaaaaa ttagtctaaa 1200

taatgaatta ctgaacccta ccggatttga acacgcgccc tgtgtattaa aggacagaca 1260

cactaccact gcgctacaaa ttcacttgct ataaaagtaa acattaaaat aaatttataa 1320

cataatattt ggtgcagaaa cttaacaatt gaacccaact tggaggccct aactatttgg 1380

gggccctagg cgaaggccta gttcgcgtaa ggccagggcc gagccaggga gggagggagg 1440

gagggaacag accagttact ttctctgtct acttgttcaa actcaatgct tctatcaacc 1500

gtcaagttct tgcttctgcc tgcttggaaa cgtgcaaagc taatccacat ctggtggtga 1560

atgcctctcc acataatcaa aggaaagatg agaaaatacg ccatatcagt gtctcttcct 1620

ttcaccacaa atgtatacaa actgtgtgta acccatgggg ccacaatcac atacttaaag 1680

tttcgaagct ttttccatgg ccattcagtg aagattcctg ggtcagaagc cattttctat 1740

tgctttctct tctctgctta atgcctctat ttctacccat tttctaatgg ctacctttct 1800

actcagaagc ttagaagacc gtataaattt tggttgggaa ggcctacatt aaatgctaat 1860

atatctattt ctctactctc acccattaat tcatgtcagt tatacttgca ctaaaggaat 1920

tcaatttttc acgcccattt gcctaacata gtgaattgtt tctaggaacc aaaatagatc 1980

atctaattct cttctaacac ccacctatct ctcatgaatt tggtgatata ctcatttgaa 2040

atattaattt ttaaggatta ctaaaataaa tttcaaccaa actaaatttt ttttattaaa 2100

tttaatattt cggtgttttt caccgtcctt aaggtatagg gtataccagg tgaggtcatg 2160

ggggctcgaa ccttacacct ctcgctctat gagacgagag attaccggtt gaattataca 2220

gatgtgttct tttagtgcat ttgcttatca tattttaatt tggactcctc tattttacag 2280

tgattcacgt gtctcgcagg attgagtagt gaagtggcca caactgttct ttcaattaaa 2340

acaatcaggt gccaccacta aagcactcct ccttttcttc tgcttgtttt ctggtgattt 2400

ccattcatag cttcctctaa tgccctcttt tctctcttct tttaatactt gccattctca 2460

gcattgctca agtcttcgta tgctcctttc ctcctttctc ttgtttcatt tcacaacccc 2520

atgtgattca tacatgtaag acctgttatg ctttcttagg aattgatggt acaagaatca 2580

aagaaattgt atttggtggg ttagctaaaa ctaggaccct cctgtgtgct tttctaatgc 2640

tagtattggg atatgtagct caacagaatt gggatctgta gctcaactga ttgtattccg 2700

tctcaaaaaa cgggaggtcg cggattcgag ccctcgacct aactttggtg tgccctaggt 2760

gtagtgggct cacccatgag gattttacgc gcggtatccg atgtttccca cacgtaaatt 2820

cagaaagaaa aaagtaattt caattgttta ttttttagag ttgcaacttg ggtttggtca 2880

atctacactc gattaattga tctaaaaata tgggtgaact aattcaatat atataaaata 2940

taggttggtt tgagtttaga aaattttgac atgtttttaa tttgaattgg gttagaaaat 3000

atatactcac tgtgttcttt tggcttgcaa tttggacttg ccttca 3046

<210>20

<211>3523

<212>DNA

<213> calyx canthus Sinocalycanthus chinensis (Cheng et S.Y. Chang)

<400>20

attcctactt cctaatccca aaccagcccc tctgccagct gacgccatgg ctcacatgct 60

ccatcacacg caactgcaag tgctccgcca cctaaatctc cctttcctcc tcccatggcc 120

acctttctgg tacctaaata ccgcttgcgc ttttctttct aattcatcct tcacttctta 180

gatctatcta tctatctatc tatctttctt gttataacat ctctcaattt ctatctgtct 240

ctgcctcttt cgaagtgttc cattgcattc attgacactg aagattttcg aggatgagca 300

tgagggcgag gggttcttcc ccttttgctt gctttggctt ctgtaaggca tttcaggtgg 360

gactatggtc agtttggcta tctgggtttc tcttgatatg tctatccctt tataccactc 420

agagcttgcc ttcatttaga gaccatatca agagacccaa gctgaccctc agaggatttg 480

gacattctga gcaacccaac ataaccatct tctcggcacc aagtctcttt gagggtttgg 540

tgggggcaag gcagctcctg gcagtccaat catggcttgc attatcaccg tctatcatgg 600

tgattttgtt tgcacagcac ccatcatcta tcctccacat tgcagcttcc cttggtcctc 660

gagttttggt tgaagcttcc attgatttca cgtttcttgg taccccttta tttcattcca 720

tggttgcaag agcacaagca tctacttcag acatttctat tttgattgat cctgagattg 780

ttctcttgcc tgacttcatc tccaccatgc attatgtcca caagctgaag gatgattggc 840

tccttgttgg catgtcgcct aacatttctc acttcccatt tcatttggac aacactatgc 900

agcattggct tcaggaagat ggtgaacgag tagaaaacag gaagttgtgg gagtttctag 960

ttcagcaacg gcagtggagt gattgtggtg gcaaggtgct aatggcatgg aatacagggg 1020

agctgccctt gcatgcaggg gtccttcctc ctttcttata cagaaaaggg tttcacaacc 1080

aatgggttgt taatgaggca ttgtcatctg gttttaggtt tgtgtttgat gccagtgagg 1140

ccatttcaag cttctaccca gaaaatattg gtccttggcc aaaccggttc tttagaggtt 1200

ccaacaacag agatgttaaa gagagagagt gggaatttgg gggtaatttc catcttgcgt 1260

tttcatatgg atcgttctat ttaaacctgg ccaatttttc taacagacta gtaaggcttc 1320

tcaagtgtga tgggcaatat ttgttcttta acatggctga agagaatgct aacacttcta 1380

ttggacaaga tttccacaca tccatcaagc tgcagcaagc atcacgtcaa gagctgggga 1440

ggtccagttt aaggactgga aagatcttgc aattgaggaa agagaagaaa tggatgtctt 1500

gtgcggagga tagtttctcg ctcgacagaa aaatggactg ctcagccatg gagccatccg 1560

agaattcttt taatctgtca gggcaattag ttcttccatt ttcccttgag tcacttctac 1620

aacaaattgc tgatgacaat aggtctgttg tgcttgcagt tgctgggaac aattataggg 1680

acatgcttat gagttgggtt tgccggttac accacctctc tgtcacaaat ttcatcatct 1740

gtgcccttga tcctgaaatt tatcagttct ctgtactgca gggattgcct gttttcatag 1800

acccattagc tccaaccaac atcagcttca atgactgcca cttcggcaca aagtgctttc 1860

agagggtgac caaggtgaag tctcggttag tattgcagat actgaagcta ggatacaatg 1920

tcctcctgag tgatgttgat gtcttctggt tcggcaatcc tttgccatttcttctttcct 1980

ttggccctgc tgttttagta gcacagtcag atgaatacaa agagacagga cctataaact 2040

tacctcgacg attaaactct ggtttctatt ttggacgttc tgatcatact actattgctg 2100

caatggacaa ggttgtaagg cacgcattgg cttcggacct atctgaacag ccaagcttct 2160

atgatgtatt atgtggagaa ggtggtgcca accgccttgg agatgagaaa tgtcttgagc 2220

cagaaacaaa cctgacagta catttcttag acaggaatca ctttccaaat ggagcttatc 2280

aaggcctttg ggagaaggat gatgtaaaat atgcctgcat gttgcagggc tgcctcattc 2340

ttcataacaa ttggataagt gggcggaaga agaagcttga acgccagatg ctttcaggcc 2400

tgtgggagta tgatgccggc agtaggatgt gtttacagga ttggcatggg acaaagttaa 2460

tcagttttgg ttaatgaatt ctgatttgta attactcagc tttgtttttc acgatgagat 2520

gaatacaaca agtggtatct cacccttctc tgtgtttgta catagcattg gtatctatga 2580

ttattccttc aaaagctatt aaaatatgga tctagcacta cagaacaaaa gcacagaaag 2640

gcagaaaagc cttgacaaag tgagtcttaa ttgttgaatt atgttgaagc aagcactgag 2700

gctctcttcc ttccctatca actgatcatt gattacatat tccctgttct ggagatgctg 2760

aagggatacg tatgtgtgtt tggggtcacg cgatattgct gcagaaaacc gagaaggaat 2820

aaggaacttg aggaaggttc aatgtcaatt aatgtaagct cacaacaaga cttcactgta 2880

agaatcatcc atgccggtgg gatcatagaa aggtatgaag gtgtggttcg tgctagtcgt 2940

ttgatggaga agtacccgaa gatgtgtatt gcaaagcctg aagttttcaa gaagccacat 3000

gagtccatca tcagggcaga tgagtacctg ttgccgggtc aaaaatttta cttggtccct 3060

cagtctacaa tgaagaaact gaagcgtaag caccctgaaa agtttcaagt aggcaatgac 3120

aagcagagca acacattgga atatatggat aggaagaaaa tgcttgattc ggaaagcggt 3180

gaggactctg atgaatctgt ttgttctgca aaggattttt atgtttctag agagaggtgg 3240

tctagatgtg ttcaaggatc caataagccc ttcactccac ccattaagaa ggtaaaaatg 3300

tggaagccaa tagaatggca acctagcctt gattctgtag aagaggtctc tccatgactt 3360

ccaaccttcg tttgcatgat ttattttccc atgtttattt ttgtattctg ggcttcctcc 3420

caataacttc acaaattgtg atgagctaat aatgtttcag tacactgaga aagtatatga 3480

tgtattgcat ttgtcgattg gccccctgaa ggcataccat tga 3523

<210>21

<211>930

<212>DNA

<213> calyx canthus Sinocalycanthus chinensis (Cheng et S.Y. Chang)

<400>21

accaatagta gagacaccca agaggtatgg cgactgtttg ctttatcagt aaacactatt 60

atgtgtgcag ctaaatttca ggtgcattaa tttctaaaca taaaagcatg cagatgagtg 120

gcaccgatat gtacacagtg aatatgtgca ttaatttaat ttttggtttt gataatcaat 180

taccatgttc atgtggcatt ctttttagtg gttttgctga agcttgtgaa tgtttcattt 240

ctaaggactt tttttttgtt gaacactttt tgtataactc attacaattc ttgatatatt 300

ttttttaaaa tagcagtagg gagtgcattc tttttatatc tatgacgagg acctaatgta 360

gtctgcttat gtcgtcgtcg tcgtcgtcat catcagccta tatcccatca aagtagtcta 420

cttatgtcct gagctctttt agtttttctt tgcctgtcaa ggatgttgat gcttttccct 480

tgcaaaattc tgctgacaat tttagtactc ctttttcttc atgcttctgc aatggcctga 540

gacttgacct tgattttatt gatgccctca tctaatttat gattggagtt ctgttaattt 600

tgttgttgtc tcccccacca ccccaaaccc ccaacccaca aaaaaaaaag gagcattgct 660

tagcttctct agtcataact ctgcctcata tctttgtgct catcatcctt ttttttttgg 720

ttgtccatgt gactgcataa ctacaagcat ggcggaccca atttagagaa tctagaatgg 780

ccaagtttga atctttgtgg tcatagatgt tcttttaact tggaactttt gtgttgttat 840

ctgcagaaca atctttagga cttgattctg aaatctacat ggtctttgac atctttgttc 900

acctgcagat accatacatg caaggtatgg 930

<210>22

<211>970

<212>DNA

<213> calyx canthus Sinocalycanthus chinensis (Cheng et S.Y. Chang)

<400>22

gccgagccac catttgggtt catgcctttg cattgagaaa aagtctccgg agtttggatc 60

aagaaaatag ttgttgacta ttccatggta agacggatag aggccagtgg caatcgagta 120

gtggttgggg aatgtgaggg tcgggaagac ggggatgaga ccggtttcgg cttcagttcc 180

attcgcaata aggcgggcaa tgttgggggt tggggtcttg aattggtaac cgaaacggaa 240

cccatcagtg gagatgagta gaacaatggg tcgccggagt ttggtaagag ggcgggcagt 300

caattgagga gaattttggg aggattggct tgaatgggat tcagaagagg aggagaagaa 360

taggaaagca aaagcgatgg aggtggcgag agctaggcag gtggtgatgc caagagaggt 420

gagcagaagg gtgttatttg aggttttagg agaggtgatg ggagggtcat tgttgggaga 480

taagagaaga gcagtagagg ggttttgcgg gtcttcctct tctggggctg gcgttggttt 540

agaaggggct aggatggaaa aagaagcact acccatgaat ggatctttac ccctctctct 600

ctctctgaaa ggggaggtaa gagatgaagg attggagatg cgagaatttc agaaattgaa 660

aggtttgctg tctctgtgta tttatttttt cccccgtccc tcgaggattt ggggaaggaa 720

agagaaggga gagttggaaa aaggtaagaa agggtattgt gggtgctgtt aggaagaaag 780

tttggtgtag agtcgtgatt gagtaaattc aaagaaagaa ggggatgtgc tctgcttgga 840

tctctttctt tccgctgctg ttacctgtat cattcgcttg tttcgagaga ttcacttgga 900

agaagaggag gagaagcgtt tggattggtc ttcttgatgt aagattccag cgctttggtc 960

ccactagaga 970

<210>23

<211>1829

<212>DNA

<213> calyx canthus Sinocalycanthus chinensis (Cheng et S.Y. Chang)

<400>23

gatttggtca gcaaaggatc ctgattcgtt gagatcttta ctcgtactaa attactgggg 60

cccacacaaa cacacgtcac ctgccccagt gcccccacat aaaatgggtc cgttcacaat 120

tgggtagtca cgcggagtgt ccaacagttt ttgcttttcg tgtacatctc ttttccgcac 180

ttcggcctct tcacaatttc accgccaaca gataataagc gttcttcctt cccatttcac 240

ccacaaatcc ccaccacctc cttaagatcc ccactctcag tctctcctct cccactccca 300

ctcccactcc cattccccttttcttctttg ttcttttgcc ttcaatggat tcccttctcc 360

tcttcttcac cacggccatt ttgggctcag ccctgtactg gttcgtctgc gtgcttggct 420

cagcagagca gaaaggcaag cgggcgatca acctaaccag cggctccatc tctagagaga 480

aagttcatga caaatacaag cagtactggt ccttcttccg tcgccccaag gagatcgaga 540

ctgcagagat agtgccccaa ttcgtagata ccttctacaa tctcgtcact gacatatacg 600

aatggggctg ggggcagagc ttccacttct ccccttccat tcccggaaag tcccacaagg 660

acgccacccg tctccacgaa cagatggccg tagatcttct ccgcgtcggc cccggcaacc 720

gaatcgtgga cgtcggatgc ggagtagggg gccccatgcg cgcaatcgcc gcccactcta 780

aggcccatgt caccggcatc accatcaacg agtatcaagt aaagagggcc aaggcccaca 840

acaagaaggc gggcctgggc gacctctgcg acgtcgtctg cggcaacttc ctccagatgc 900

cgttcccaga ctcatccttc gatggggcct attccatcga ggcgacgtgt cacgctccaa 960

gcctcgaaga tgtctacgcc gagatcttcc gtgtcctccg ccccggggcc ctctacgtct 1020

cctacgagtg ggtcaccacc gacaagtacc aatccgacaa ccccgtgcac atcgagaccg 1080

tgcaggggat cgagcggggc gatgcgctgc cgggcctccg gcgatacgac cagatctccc 1140

aaattgcgaa gaaggtcggg tttgaagttg tcgaggagcg ggatctggcc cggcctccag 1200

ccggagagtg gtggaagagg ctcaaaatgg gtcgaatcgc ctactggagg aaccatgttg 1260

tggtcaccgt gctcacattg ctcggagttg cccctaaagg tgtggtggac gttcacgaga 1320

tgctgttcaa gaccgccgga tacctgaccc acggcggcga gaccggaatt tttactccga 1380

tgcatatgat tctctgcaga aagcccgcaa actaggttct ctcctttttt ttattactct 1440

cggtagtttt ttttgttaaa cctttctttc taagttattt tgattatgtt tttatgttat 1500

ttttgttctg tttggtgatt ggaactttgg aagaagggat tggagatggg tttttaattt 1560

tattttttaa ttttattttg gaatgtggtg tggtggactg ggggagatgt ttaatgtgtg 1620

gaagaggaga aagatggtaa tttttttgga aatctgcact aatttttatt ctacctttta 1680

gtttctactg ctgccttttt acttgctttt catgacaatg atgattcttg gtaacttata 1740

acgaatcttc tttattcttt ttaacctttc taacattaga aattgagatg tggagatgct 1800

tggaatgttt aagatttgta gcgtacggg 1829

<210>24

<211>21

<212>DNA

<213> Artificial sequence

<400>24

gaggagaaag gcgagaaggt a 21

<210>25

<211>23

<212>DNA

<213> Artificial sequence

<400>25

caaccctaac caaaacccta aat 23

<210>26

<211>23

<212>DNA

<213> Artificial sequence

<400>26

atattagggg ccttcatagc gta 23

<210>27

<211>23

<212>DNA

<213> Artificial sequence

<400>27

gccgtggtat aattgatttg gta 23

<210>28

<211>23

<212>DNA

<213> Artificial sequence

<400>28

gagagcattt tcaagaaagg tca 23

<210>29

<211>23

<212>DNA

<213> Artificial sequence

<400>29

gtgaacaaag ttaccatcca tcc 23

<210>30

<211>23

<212>DNA

<213> Artificial sequence

<400>30

cttttggtgt tttggaaata tgg 23

<210>31

<211>23

<212>DNA

<213> Artificial sequence

<400>31

tcagaacaaa tggaaatgca ata 23

<210>32

<211>23

<212>DNA

<213> Artificial sequence

<400>32

tcacctcctt gagttttgtt ttt 23

<210>33

<211>23

<212>DNA

<213> Artificial sequence

<400>33

tgtgtcgacc tctaatttaa cca 23

<210>34

<211>23

<212>DNA

<213> Artificial sequence

<400>34

aagccctcaa ttccattttt atc 23

<210>35

<211>23

<212>DNA

<213> Artificial sequence

<400>35

caatgaggag ttgttacagg cat 23

<210>36

<211>23

<212>DNA

<213> Artificial sequence

<400>36

gtggaaaact attgctttca tgg 23

<210>37

<211>23

<212>DNA

<213> Artificial sequence

<400>37

cacaagaaaa ccatatgcag tga 23

<210>38

<211>21

<212>DNA

<213> Artificial sequence

<400>38

ggaagaaaat gaggatctcc g 21

<210>39

<211>22

<212>DNA

<213> Artificial sequence

<400>39

aacaatcctc cacatcttca gc 22

<210>40

<211>23

<212>DNA

<213> Artificial sequence

<400>40

atttcgacca tctgcaagaa taa 23

<210>41

<211>23

<212>DNA

<213> Artificial sequence

<400>41

agacaagctt ttggagtgtt tca 23

<210>42

<211>23

<212>DNA

<213> Artificial sequence

<400>42

tattaagcag tgggaccttt tga 23

<210>43

<211>23

<212>DNA

<213> Artificial sequence

<400>43

ctttgttcac gaaccttttc ttc 23

<210>44

<211>23

<212>DNA

<213> Artificial sequence

<400>44

gaatggaggt tgaagagaag agg 23

<210>45

<211>23

<212>DNA

<213> Artificial sequence

<400>45

agatatgagt tcctgatccc cag 23

<210>46

<211>23

<212>DNA

<213> Artificial sequence

<400>46

gaaactgtct ttcaccgtta cct 23

<210>47

<211>23

<212>DNA

<213> Artificial sequence

<400>47

acatgaccct catcatcttc aat 23

<210>48

<211>23

<212>DNA

<213> Artificial sequence

<400>48

aagagaatca aacgaatggg aat 23

<210>49

<211>23

<212>DNA

<213> Artificial sequence

<400>49

aagtcattga agcgagagat gag 23

<210>50

<211>23

<212>DNA

<213> Artificial sequence

<400>50

tattgtccca agctttcttt caa 23

<210>51

<211>23

<212>DNA

<213> Artificial sequence

<400>51

tccatcagat ccaataagta ccg 23

<210>52

<211>23

<212>DNA

<213> Artificial sequence

<400>52

ccatgttcca aaacatacac ctt 23

<210>53

<211>23

<212>DNA

<213> Artificial sequence

<400>53

aataaggaaa cttgtccccg taa 23

<210>54

<211>24

<212>DNA

<213> Artificial sequence

<400>54

ttcaaagtag ctcaagcatt tgtc 24

<210>55

<211>22

<212>DNA

<213> Artificial sequence

<400>55

agttggtcat gcgattcaaa at 22

<210>56

<211>23

<212>DNA

<213> Artificial sequence

<400>56

tgggtgaatt atgctgtgta atg 23

<210>57

<211>23

<212>DNA

<213> Artificial sequence

<400>57

aagaccttgt aaaaaccagt ccc 23

<210>58

<211>23

<212>DNA

<213> Artificial sequence

<400>58

gcagagaact ggtgtcttag gaa 23

<210>59

<211>23

<212>DNA

<213> Artificial sequence

<400>59

gagacattga atcccaacaa gac 23

<210>60

<211>23

<212>DNA

<213> Artificial sequence

<400>60

aacttggagg ccctaactat ttg 23

<210>61

<211>23

<212>DNA

<213> Artificial sequence

<400>61

agcaagaact tgacggttga tag 23

<210>62

<211>23

<212>DNA

<213> Artificial sequence

<400>62

cttgcgcttt tctttctaat tca 23

<210>63

<211>23

<212>DNA

<213> Artificial sequence

<400>63

atcctcgaaa atcttcagtg tca 23

<210>64

<211>23

<212>DNA

<213> Artificial sequence

<400>64

gacgaggacc taatgtagtc tgc 23

<210>65

<211>23

<212>DNA

<213> Artificial sequence

<400>65

aaaagcatca acatccttga cag 23

<210>66

<211>23

<212>DNA

<213> Artificial sequence

<400>66

aggatggaaa aagaagcact acc 23

<210>67

<211>23

<212>DNA

<213> Artificial sequence

<400>67

caatccttca tctcttacct ccc 23

<210>68

<211>23

<212>DNA

<213> Artificial sequence

<400>68

taataagcgt tcttccttcc cat 23

<210>69

<211>23

<212>DNA

<213> Artificial sequence

<400>69

ttgaaggcaa aagaacaaag aag 23

Claims (2)

1. A Chimonanthus nitens EST-SSR marker is characterized in that: includes 23 microsatellite markers whose numbering, nucleotide sequence, primer sequence and characteristics are shown in the following table:

the sequence numbers of the nucleotide sequence and the primer sequence are the numbers in the sequence table.

2. Use of the prune EST-SSR marker of claim 1 in the analysis of prune genetic diversity.

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