CN107118588B - A kind of method and apparatus for extracting marine streptomyces natural blue pigment - Google Patents
A kind of method and apparatus for extracting marine streptomyces natural blue pigment Download PDFInfo
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- CN107118588B CN107118588B CN201710306411.7A CN201710306411A CN107118588B CN 107118588 B CN107118588 B CN 107118588B CN 201710306411 A CN201710306411 A CN 201710306411A CN 107118588 B CN107118588 B CN 107118588B
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- marine streptomyces
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- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
- C09B61/00—Dyes of natural origin prepared from natural sources, e.g. vegetable sources
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Abstract
The present invention provides a kind of method and apparatus for extracting marine streptomyces natural blue pigment, including feeding port, control panel, fluid reservoir, spray equipment, agitating device, Suction filtration device, ultrasonic disruption tank, extractor, tophan box, crude liquid collecting tank and pH purified pool tank, the extracellular fermentation liquid of marine streptomyces and the two different cyanine correspondences of streptomycete intracellular are taken into different processing methods, the characteristics of its cohesion form changes under different pH value using the cyanine of marine streptomyces, purifying is extracted to it repeatedly, filtration efficiency is reduced simultaneously to prevent cell from accumulating on filtering film, devise agitating device, the design of agitating device increases the streptomycete rate of filtration intracellular and simplifies filter operation, the method and apparatus provided by the invention for extracting marine streptomyces natural blue pigment are to ocean The utilization rate of streptomycete is big, and production efficiency is fast, and the purity of gained cyanine is higher.
Description
Technical field
The present invention relates to microorganisms pigments fields, and in particular to it is a kind of extract marine streptomyces natural blue pigment method and
Device.
Background technique
Pigment is widely used in the industrial productions such as food, medicine, cosmetics, health care product in large quantities.As people live
Horizontal raising, the demand of pigment increased dramatically, and synthetic food color is not because having nutritive value and having toxic action whole
It will be unable to capture pigment market.Compared to animals and plants pigment, microorganisms pigments have not by the factors shadow such as time, space and environment
Loud advantage can use installation for fermenting and is mass produced.Blue has just been doomed answering for cyanine as one of three primary colors
Be with prospect and potentiality it is huge, cyanine can arrange in pairs or groups it is red, uranidin modulate multiple color tones pigment.
Microorganisms pigments as a kind of natural pigment because its safety, coloring is natural, have no toxic side effect many advantages, such as, in state
Border market sales revenue annual growth is maintained at 10% or more.Currently, the pigment of food applications is mainly red, uranidin, cyanine
More rare, to solve this problem, foreign countries were dedicated to researching and developing natural pigment since the sixties.Wherein, microorganism
Cyanine is exactly the research hotspot of a researcher, is identified and isolated from out a variety of micro- lifes that can synthesize cyanine
Object, including Streptomyces sp.ZLT (Streptomyces sp.), Pseudoalteromonas (Pseudoaltermonas sp.E18), Du
Gan formula bacterium B2 bacterial strain (Duganella sp.B2), Aureobasidium pullulans (Aureobasidium pullulans), Erythrobacillus
(Rhodobacter) and purple nonsulfur bacteria belongs to etc..
On industry requirement, microbe fermentation method prepare cyanine can be very good to solve pigment less varieties, source it is opposite
Insufficient and at high cost problem.But current cyanine industrialized production is there is also production technology is not perfect, energy consumption
Greatly, labor productivity is low, yield and the problems such as purity and quality are not high enough, so being badly in need of from new process, new clothes
It sets and high-efficiency strain screens several aspects such as domestication to solve these problems, to reach the economic benefit of increasing both production and income.
Summary of the invention
The present invention provides a kind of method and apparatus for extracting marine streptomyces natural blue pigment, and marine streptomyces can be simultaneously
Generate extracellular fermentation liquid and the two different fat-soluble and water-soluble cyanine of streptomycete intracellular, the present invention for this two
Kind different cyanine correspondences take different processing methods, using such cyanine under different pH value its agglomerate form
The characteristics of changing purifies it.
It is a kind of extract marine streptomyces natural blue pigment method the following steps are included:
A. the extracellular fermentation liquid of marine streptomyces and streptomycete intracellular are separated；
B. the streptomycete intracellular separated is subjected to clasmatosis, then the streptomycete and cell wall intracellular that will be crushed
Separation；
C. by extracellular fermentation liquid acid isolated in the streptomycete intracellular separated in step b and step a
Property solution adjusts its pH value to 2~5 to get to the solid-state cyanine settled under acidity.
Preferably, pH value described in step c is 4.
Preferably, acid solution described in step c is hydrochloric acid.
A kind of device extracting marine streptomyces natural blue pigment, including fluid reservoir and controller, the fluid reservoir it is upper
End is equipped with feeding port, and the lower part of fluid reservoir is equipped with spray equipment, and the lower section of spray equipment is equipped with Suction filtration device, Suction filtration device and spray
Agitating device is equipped between shower device, the bottom end of agitating device is equipped with rotary blade, and controller passes through spray motor control spray dress
The spray rate set, controller control the rotary rpm of the rotary blade of agitating device by stirring motor.
Suction filtration device includes suction funnel, filters film and suction filter pump, and suction funnel is inverted triangle structure, filters film setting and exists
On the top margin of the inverted triangle of suction funnel, the rotary blade of agitating device keeps micro- with the upper plane for filtering film and contacts, pivoting leaf
Wheel can be rotated in the Plane Rotation that the micro- contact in the top of suction filtration film filters film, and rotary blade is for preventing suction filtration film from blocking and adding
Fast filtering, suction filter pump, which can drive, to be filtered substance to be filtered on film and moves downward, the lower section of suction funnel be equipped with crude liquid collecting tank and
Ultrasonic disruption tank can enter ultrasonic wave by suction funnel under the driving of suction filter pump by filtering the streptomycete intracellular of film
Broken tank cannot enter crude liquid collecting tank along the outer of suction funnel by filtering the extracellular fermentation liquid of film.
Extractor is connected with below the ultrasonic disruption tank, the upper end of extractor is equipped with extract liquor injection port, ultrasound
Broken cell slurries are obtained after wave is broken, including cyanine intracellular and cell wall residue, are injected by extract liquor injection port
The extract liquor that cyanine intracellular can be extracted, mixes to come into full contact with extract liquor with broken cell slurries, is equipped in extractor
Horizontal mixer, is connected with tophan box below extractor, and extraction can be had the clear liquid of streptomycete intracellular and thin by tophan box
Cell wall separation, the bottom end of tophan box are equipped with discharge gate, and the side of tophan box is connected with vacuum filtration pump, the working method of tophan box
For controllable intermittent running, the tail end that pump is filtered by vacuum is connect with the crude liquid collecting tank, and extraction is had indigo plant by vacuum filtration pump
The clear liquid of pigment pumps in crude liquid collecting tank.
PH purified pool tank is connected with below the crude liquid collecting tank, the upper side of pH purified pool tank is provided with pH
The bottom end of regulator injection port, pH purified pool tank is provided with discharge port.
Preferably, the controller includes control panel.
Preferably, the aperture for filtering film is 0.22 μm, can be by the cell free fermentation liquid and chain intracellular of marine streptomyces
The two different cyanine separation of mould.
Preferably, be equipped with monitoring temperature regulating device in the ultrasonic disruption tank, monitoring temperature regulating device can monitoring temperature simultaneously
Temperature is controlled, makes temperature control in the optimum activity state of cyanine.
Preferably, the extract liquor injection port injects CCl4Extract liquor.
The invention has the following advantages:
(1) it is intracellular it is outer all there are problems that pigment, devise Suction filtration device, ultrasonic disruption tank, extractor and from
Outer two different cyanine correspondences intracellular of marine streptomyces are taken different processing methods, make ocean strepto- by heart tank
The cyanine of bacterium extracts more abundant.
(2) to solve the problems, such as to reduce filtration efficiency because of cell accumulation, devise agitating device, agitating device can be by controlling
Its revolving speed is arranged in panel processed, and agitating device can clear away the cell on filter membrane, into next device, increases the rate of filtration
And simplify filter operation.
(3) according to the cyanine of marine streptomyces, it agglomerates the characteristics of form changes, design under different pH value
PH purified pool tank injects pH adjusting agent by pH adjusting agent injection port come extraction purification cyanine.
Detailed description of the invention
Fig. 1 is the structural schematic diagram for extracting the device of marine streptomyces natural blue pigment.
Wherein, 1 is feeding port, and 2 be fluid reservoir, and 3 be spray equipment, and 4 be agitating device, and 5 be control panel, and 6 be suction filtration
Funnel, 7 is filter film, and 8 be suction filter pump, and 9 be ultrasonic disruption tank, and 10 be extractor, and 11 be extract liquor injection port, and 12 be horizontal
Blender, 13 be tophan box, and 14 be discharge gate, and 15 be vacuum filtration pump, and 16 be crude liquid collecting tank, and 17 be pH purified pool tank,
18 be pH adjusting agent injection port, and 19 be discharge port.
Specific embodiment
Technical scheme is described further in the following with reference to the drawings and specific embodiments.
The present invention provides a kind of methods for extracting marine streptomyces natural blue pigment, in order to better describe this method,
First description is used for the device of this method.
In conjunction with Fig. 1, a kind of device extracting marine streptomyces natural blue pigment, including fluid reservoir 2 and controller, fluid reservoir
2 upper end is equipped with feeding port 1, and the lower part of fluid reservoir 2 is equipped with spray equipment 3, and the lower section of spray equipment 3 is equipped with Suction filtration device, takes out
It filters and is equipped with agitating device 4 between device and spray equipment 3, the bottom end of agitating device 4 is equipped with rotary blade, the spray of spray equipment 3
Motor is electrically connected with the controller, and the stirring motor of agitating device 4 is electrically connected with the controller.
Suction filtration device includes suction funnel 6, filters film 7 and suction filter pump 8, and suction funnel 6 is inverted triangle structure, filters film 7
It is arranged on the top margin of the inverted triangle of suction funnel 6, the rotary blade of agitating device 4 keeps micro- with the upper plane for filtering film 7 and connects
The rotary blade of touching, agitating device 4 can be rotated in the Plane Rotation that the micro- contact in top for filtering film 7 filters film 7, rotary blade
It can prevent from filtering film 7 and block and accelerate to filter, suction filter pump 8, which can drive, to be filtered substance to be filtered on film 7 and move downward, and leakage is filtered
The lower section of bucket 6 is equipped with crude liquid collecting tank 16 and ultrasonic disruption tank 9, and streptomycete intracellular enters ultrasonic wave by suction funnel 6
Broken tank 9, extracellular fermentation liquid enter crude liquid collecting tank 16 along the outer of suction funnel 6.
The lower section of ultrasonic disruption tank 9 is connected with extractor 10, and the upper end of extractor 10 is equipped with extract liquor injection port 11, surpasses
Broken cell slurries are obtained after sonication, including cyanine intracellular and cell wall residue, pass through extract liquor injection port 11
Injection can extract the extract liquor of streptomycete intracellular, mix to come into full contact with extract liquor with broken cell slurries, extractor
Horizontal mixer 12 is equipped in 10, the lower section of extractor 10 is connected with tophan box 13, and tophan box 13 there can be extraction intracellular
The clear liquid and cell wall of streptomycete separate, and the bottom end of tophan box 13 is equipped with discharge gate 14, and the side of tophan box 13 is connected with vacuum
Suction filter pump 15, the working method of tophan box 13 are controllable intermittent running, the tail end and crude liquid collecting tank of vacuum filtration pump 15
16 connections, vacuum filtration pump 15 pump to the clear liquid that extraction has cyanine intracellular in crude liquid collecting tank 16.
The lower section of crude liquid collecting tank 16 is connected with pH purified pool tank 17, and the upper side of pH purified pool tank 17 is provided with
The bottom end of pH adjusting agent injection port 18, pH purified pool tank 17 is provided with discharge port 19.
Wherein, controller includes control panel, and the aperture for filtering film 7 is 0.22 μm, can be by the born of the same parents of marine streptomyces
Outer fermentation liquid and the two different cyanine separation of streptomycete intracellular, are equipped with monitoring temperature regulating device, prison in ultrasonic disruption tank 9
Surveying temperature regulating device monitoring temperature and can control temperature, make temperature control in the optimum activity state of cyanine.
In conjunction with the present apparatus, a method of marine streptomyces natural blue pigment is extracted, marine streptomyces are thrown through feeding port 1
Enter into fluid reservoir 2；The spray equipment 3 of 2 lower part of fluid reservoir sprays marine streptomyces onto the suction filtration film 7 of Suction filtration device, stirs
The rotary blade for mixing device 4 filters the Plane Rotation rotation of film 7, rotation in the micro- contact of pushed upwards marine streptomyces for filtering film 7
Rotary impeller can accelerate filter process and prevent from filtering the obstruction of film 7, can pass through the chain intracellular for filtering film 7 in marine streptomyces
Mould enters in ultrasonic disruption tank 9 through filtering film 7, can not pass through the cell free fermentation liquid edge of suction filtration film 7 in marine streptomyces
The outer of suction funnel 6 enter crude liquid collecting tank 16, so far by the extracellular fermentation liquid of marine streptomyces and strepto- intracellular
Bacterium is separated.
Streptomycete intracellular carries out ultrasonication, supersonic frequency 300W, between every ultrasonic ten seconds in ultrasonic disruption tank 9
Every ten seconds, continue 20 minutes；Cell slurries (streptomycete and cell wall intracellular) after ultrasonication are pumped to extractor
In 10, CCl is injected by extract liquor injection port 114Extract liquor, horizontal mixer 12 is by cell slurries and CCl4Extract liquor is sufficiently mixed
It closes；After completing extraction, by ultrasonication and after extract liquor is added, obtained mixed liquor is pushed in tophan box 13, centrifugal rotational speed
Mixed liquor is subjected to centrifugal treating under 12000r/min, obtain upper layer extraction have streptomycete intracellular clear liquid and lower layer it is thin
Cell wall residue；The extraction on upper layer has the clear liquid of streptomycete intracellular to be extracted into crude liquid collecting tank 16 by vacuum filtration pump 15, lower layer
Cell wall residue be discharged through discharge gate 14.The streptomycete intracellular that will be separated has carried out clasmatosis, then will be crushed
Streptomycete intracellular and cell wall separation.
Liquid in crude liquid collecting tank 16 is pumped into pH purified pool tank 17, because it is solidifying under different pH value for cyanine
Combinate form state changes, and puts into the hydrochloric acid solution that concentration is 2mol/L by pH adjusting agent injection port 18 to get arriving under acidity
The cyanine of sedimentation, by this solid cyanine through 19 output of discharge port.
Certainly, the above description is not a limitation of the present invention, and the present invention is also not limited to the example above, this technology neck
The variations, modifications, additions or substitutions that the technical staff in domain is made within the essential scope of the present invention also should belong to of the invention
Protection scope.
Claims (3)
1. a kind of device for extracting marine streptomyces natural blue pigment, which is characterized in that including fluid reservoir and controller, the storage
The upper end of flow container is equipped with feeding port, and the lower part of fluid reservoir is equipped with spray equipment, and the lower section of spray equipment is equipped with Suction filtration device, filters
Be equipped with agitating device between device and spray equipment, the bottom end of agitating device is equipped with rotary blade, the spray motor of spray equipment with
Controller electrical connection, the stirring motor of agitating device are electrically connected with the controller；
Suction filtration device includes suction funnel, filters film and suction filter pump, and suction funnel is inverted triangle structure, filters film setting and is filtering
On the top margin of the inverted triangle of funnel, the rotary blade of agitating device keeps micro- with the upper plane for filtering film and contacts, suction funnel
Lower section is equipped with crude liquid collecting tank and ultrasonic disruption tank, can be passed through under the driving of suction filter pump by filtering the streptomycete intracellular of film
Suction funnel enters ultrasonic disruption tank, cannot be entered slightly by filtering the extracellular fermentation liquid of film along the outer of suction funnel
Liquid collecting tank；
It is connected with extractor below the ultrasonic disruption tank, the upper end of extractor is equipped with extract liquor injection port, in extractor
Equipped with horizontal mixer, tophan box is connected with below extractor, extraction there can be the clear liquid of streptomycete intracellular by tophan box
It is separated with cell wall, the bottom end of tophan box is equipped with discharge gate, and the side of tophan box is connected with vacuum filtration pump, pump is filtered by vacuum
Tail end is connect with the crude liquid collecting tank；
PH purified pool tank is connected with below the crude liquid collecting tank, the upper side of pH purified pool tank is provided with pH adjusting
The bottom end of agent injection port, pH purified pool tank is provided with discharge port；
The controller includes control panel；
Monitoring temperature regulating device is equipped in the ultrasonic disruption tank.
2. a kind of device for extracting marine streptomyces natural blue pigment according to claim 1, which is characterized in that the pumping
The aperture of filter membrane is 0.22 μm.
3. a kind of device for extracting marine streptomyces natural blue pigment according to claim 1, which is characterized in that the extraction
Liquid injection port is taken to inject CCl4Extract liquor.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710306411.7A CN107118588B (en) | 2017-05-04 | 2017-05-04 | A kind of method and apparatus for extracting marine streptomyces natural blue pigment |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710306411.7A CN107118588B (en) | 2017-05-04 | 2017-05-04 | A kind of method and apparatus for extracting marine streptomyces natural blue pigment |
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| CN107118588B true CN107118588B (en) | 2019-05-24 |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103602615A (en) * | 2013-11-13 | 2014-02-26 | 嘉兴学院 | Streptomyces Iividans and application thereof |
| CN106497980A (en) * | 2016-10-11 | 2017-03-15 | 嘉兴学院 | A kind of extracting method of natural blue pigment and colouring method |
| CN206814678U (en) * | 2017-05-04 | 2017-12-29 | 山东科技大学 | A kind of device for extracting marine streptomyces natural blue pigment |
-
2017
- 2017-05-04 CN CN201710306411.7A patent/CN107118588B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103602615A (en) * | 2013-11-13 | 2014-02-26 | 嘉兴学院 | Streptomyces Iividans and application thereof |
| CN106497980A (en) * | 2016-10-11 | 2017-03-15 | 嘉兴学院 | A kind of extracting method of natural blue pigment and colouring method |
| CN206814678U (en) * | 2017-05-04 | 2017-12-29 | 山东科技大学 | A kind of device for extracting marine streptomyces natural blue pigment |
Non-Patent Citations (2)
| Title |
|---|
| 天蓝色链霉菌胞内蓝色素提取方法研究;张和春等;《生物技术》;20000630;第10卷(第3期);19-22 |
| 海洋链霉菌M259高产蓝色素理化性质研究;邓祥元等;《食品科学》;20061231;第27卷(第7期);35-39 |
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Granted publication date: 20190524 Termination date: 20210504 |