CN107115905B - A kind of the batch calibrating installation and calibration method of hand-held pipettor capacity - Google Patents
A kind of the batch calibrating installation and calibration method of hand-held pipettor capacity Download PDFInfo
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- CN107115905B CN107115905B CN201710318255.6A CN201710318255A CN107115905B CN 107115905 B CN107115905 B CN 107115905B CN 201710318255 A CN201710318255 A CN 201710318255A CN 107115905 B CN107115905 B CN 107115905B
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- pipettor
- electric cylinders
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/02—Burettes; Pipettes
- B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0605—Metering of fluids
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/14—Process control and prevention of errors
- B01L2200/148—Specific details about calibrations
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- Health & Medical Sciences (AREA)
- Clinical Laboratory Science (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Fluid Pressure (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
The invention discloses a kind of batch calibrating installation of hand-held pipettor capacity and calibration methods, electronic slide unit is driven to vertically move by machine control modules, electric cylinders push rod is flexible, mutual movement of electrical turntable rotation etc. realizes that manipulator takes liquid to pipettor, transfer, drain operation, the pressure feedback of pressing pipettor button is acquired to host computer by pressure sensor, host computer simulates manually pressing operation by respective algorithms, pass through electronic balance, the acquisition of temperature sensor realization liquid relief data, and program is write in host computer according to vertification regulation " JJG 646-2006 " and realizes data processing.Drip problem that may be present that present invention eliminates pipettors in transfer process, it realizes the mass when single calibration point calibrates pipettor, automate, bring error when reducing artificial repetitive operation and big data quantity processing, while calibration data can be analyzed and provide Calibration Report.
Description
Technical field
The present invention relates to pipettor field more particularly to a kind of hand-held pipettor capacity batch calibrating installation and calibration sides
Method, this method and device relate generally to calibrate (in defined condition liquid-transfering gun capacity relative error and capacity repeatability
Under, the standard that can refer to one to the characteristic assignment of the measuring appliance including reference material, and determines that its indicating value is missed
Difference).
Background technique
Pipettor be widely used in Biochemical Lab, environmental laboratory, food analytical laboratories, hospital, health and epidemic prevention station,
In blood donor center, belong to liquid precision sample apparatus, rapid, accurate quantitative sampling can be carried out to a small amount of fluid sample and test solution
And sample-adding, for adjustable pipette, operator can also adjust the capability value of pipettor according to actual needs.Pipettor conduct
Necessary equipment when liquid relief, the calibration of capacity directly affect measurement result, but in the long-term use, operation, temperature, people
Its precision can all be impacted for factor etc., it is insufficient or excessive that pipettor not up to standard has a liquid relief in use, can
Production accident or research achievement deviation can be will lead to, to guarantee that result data has good precision, accuracy and confidence level,
Periodic calibration must be carried out to it.
Traditional pipettor calibration method uses measurement method, that is, weighs the pure water released in the tested a certain scale of pipettor
Quality, then thus and the density of water acquires the actual volume of tested pipettor, and be compared with a calibrating point capacity.Due to root
It needs to detect 6 times according to each capacity calibrating point of national rule, 3 capability values for detecting an adjustable pipette need to carry out 18
It is secondary that liquid and drain is taken to operate, it manually presses pipettor difficulty or ease and guarantees that the degree pressed every time all meets measurement verification regulations.
Therefore, this method operating procedure repeats, is complicated, and manual operation can bring certain error, in the shifting of calibration batch time
When liquid device, these defects are become apparent.
Hardware aspect, the high-freedom degree simulation manipulator that foreign countries have part company to produce may be implemented to move to a certain extent
The operation of liquid device, however mechanical excessive freedom degree, excessive freedom degree are not needed in the actual use of calibration pipettor
It will cause waste, and high expense also counteracts its application in pipettor calibration.Software aspects state, it is interior designed
Pipettor calibration system is also being constantly progressive, and can the mass data of acquisition is efficiently treated through and be analyzed, however only real
The automation for having showed data processing, in the automation that pipettor controls, there is also vacancies.
Summary of the invention
The purpose of the present invention is to provide a kind of hand-held pipettor capacity batch calibrating installation and calibration methods, solve back
Defect existing for scape technology calibration method.
The purpose of the present invention is what is be achieved through the following technical solutions: a kind of batch calibration cartridge of hand-held pipettor capacity
It sets, including host computer, electric cylinders, electrical turntable, cross bracket, horizontal bracket, frame, and is vertically installed at frame side electricity
Dynamic slide unit, is mounted on relatively another side rails, is mounted on the gentle container being placed on balance in day of bottom surface, has liquid in container
And temperature sensor.Sliding block is installed, the electronic slide unit is made of straight line slide unit and drive motor, the water on the guide rail
One end of flat bracket is fixedly connected with the straight line slide unit, and the other end is connect with sliding block.The electric cylinders are mounted on a horizontal bracket,
Output shaft passes through horizontal bracket from top to bottom, and end installation by adhering has pressure sensor；The electrical turntable is mounted on level
The bottom surface of bracket, the cross bracket are connected with the output shaft of electrical turntable, and four branches ends of cross bracket have
The fixture of fixed pipettor.The length of the branch is equal to the horizontal distance between electric cylinders and electrical turntable output shaft.The electricity
The output shaft of cylinder and the container are located on same vertical axis.The electric cylinders, electrical turntable, electronic slide unit, pressure sensor,
Temperature sensor and balance are connected with host computer respectively.
A kind of calibration method of claim 1 described device, comprising the following steps:
(1) 4 pipettors to be checked are fixed on cross bracket by fixture, and set the range V1 of pipettor；
Pass through the electronic slide unit of PC control, electrical turntable, electric cylinders integral reset, electrical turntable rotational angle A1, so that electric cylinders is defeated
Shaft is directed at first pipettor button to be checked；
(2) worked by PC control electric cylinders so that with pressure sensor output end pressing be calibrated pipettor by
Button, until being pressed into complete halt；During pressure sensor acquisition pressing, the pressure of button press, and by pressure
Value feeds back to host computer.According to pressure value, acquisition is calibrated pipettor (8) button and presses the stage and second first host computer
Pressure value F1, F2 in pressing stage, the first pressing stage are to start to be pressed into the stage for being depressed into the first halt；Second presses
The pressure stage is the stage that the first halt is depressed into complete halt, and PC control electric cylinders reset.
(3) PC control electric cylinders work, when the collected contact pressure value of pressure sensor is greater than F1, host computer control
Electric cylinders processed stop.The electronic slide unit of PC control, so that the vertical dropping distance X1 of straight line slide unit, is calibrated the suction of pipettor at this time
Head 2 to 3mm under vessel level at.PC control electric cylinders reset, and draw liquid into suction nozzle, wait PC control electricity after 2s
Dynamic slide unit resets.
(4) host computer obtains initial mass value m1 by electronic balance.
(5) the electronic slide unit of PC control, so that the vertical dropping distance X2 of straight line slide unit, is calibrated the suction of pipettor at this time
Head 2 to 3mm above the vessel level at.PC control electric cylinders output shaft is mobile, when the collected contact pressure of pressure sensor
When force value is greater than F1, PC control electric cylinders stop, and wait 2s, and PC control electric cylinders output shaft is mobile, works as pressure sensor
When collected contact pressure value is more than or equal to F2, PC control electric cylinders stop.Pipettor arranges the liquid in suction nozzle at this time
It removes.
(6) host computer obtains final mass value m2 by electronic balance.
(7) host computer obtains liquid actual temperature t in container by temperature sensor, obtains corresponding under fluid temperature
Calibration factor related coefficient K (t) obtains actual volume v '=K (t) (m2-m1).
(8) it is resetted by the electronic slide unit of PC control, electric cylinders.
(9) k measurement is carried out according to step 3~8, obtains the volume number that first pipettor is measured at range V1
According to for v111 '~v11k '；
(10) host computer successively controls electrical turntable and rotates forward 90 °, surveys according to step 2-9 to remaining three pipettor
Examination, obtains its volume data of excess-three branch pipettor at range V1 respectively；
(11) range for adjusting 4 pipettors obtains body of 4 pipettors at range V2, V3 according to step 2-10
Volume data；
(12) according to measurement volume and range, the capacity relative error and capacity repeatability of pipettor are obtained.Wherein, i-th
Capacity relative error of the branch pipettor at range Vj are as follows:
Wherein
v′ijnFor n-th experimental data of i-th pipettor at range Vj.
Capacity repeatability of i-th pipettor at range Vj are as follows:
Wherein
Wherein, i indicates the serial number for being calibrated pipettor, and j indicates the range serial number being calibrated, and k expression is tested
Further, the first pressing staged pressure value F1 is the maximum pressure value measured in the first pressing stage；Second
Pressing staged pressure value F2 is the maximum pressure value measured in the second pressing stage.
The beneficial effects of the present invention are: the present invention is realized for the automatic of hand-held pipettor capacity batch calibration
Change, national inspection is met so that the operation of two halts of pressing pipettor button is effectively controlled based on the analysis for surveying pressure
Set pattern journey.The repetitive operation of calibration person is greatly reduced, while reducing manually-operated error.
Detailed description of the invention
Fig. 1, Fig. 2 are the structural schematic diagram of calibrating installation of the present invention；
Schematic diagram when Fig. 3 is device entire lowering；
Fig. 4 is electric cylinders schematic device；
Fig. 5 is pipettor button press pressure curve；
In figure, frame 1, electronic slide unit 3, straight line slide unit 4, horizontal bracket 5, electrical turntable 6, guide rail 7, is calibrated bottom plate 2
Pipettor 8, electric cylinders 10, pressure sensor 11, electronic balance 12, container 13, temperature sensor 14, is slided the bracket 9 of fixed electric cylinders
Block 15, cross bracket 16.
Specific embodiment
A kind of batch calibrating installation of hand-held pipettor capacity, including host computer, electric cylinders 10, electrical turntable 6, cross
Shape bracket 16, horizontal bracket 5, frame 1, and it is vertically installed at the electronic slide unit 3 in 1 side of frame, it is mounted on the opposite other side and leads
Rail 7 is mounted on the balance 12 of bottom surface and the container 13 being placed on balance, has liquid and temperature sensor 14 in container 13.Institute
It states and sliding block 15 is installed on guide rail 7, the electronic slide unit 3 is made of straight line slide unit 4 and drive motor, the horizontal bracket 5
One end is fixedly connected with the straight line slide unit 4, and the other end is connect with sliding block 15.The electric cylinders 10 are mounted on horizontal bracket 5,
Output shaft passes through horizontal bracket 5 from top to bottom, and end installation by adhering has pressure sensor 11；The electrical turntable 6 is mounted on
The bottom surface of horizontal bracket 5, the cross bracket 16 are connected with the output shaft of electrical turntable 6, and four points of cross bracket 16
Branch end has the fixture of fixed pipettor.The branch divide length be equal between electric cylinders and electrical turntable output shaft it is horizontal away from
From.The output shaft of the electric cylinders and the container 13 are located on same vertical axis.The electric cylinders 10, electrical turntable 6, electric sliding
Platform 3, pressure sensor 11, temperature sensor 14 and balance 12 are connected with host computer respectively.
A kind of batch calibration method of hand-held pipettor capacity, comprising the following steps:
(1) 4 pipettors to be checked are fixed on cross bracket 16 by fixture, and set the range of pipettor
V1；By the electronic slide unit 3 of PC control, electrical turntable 6,10 integral reset of electric cylinders, 6 rotational angle A1 of electrical turntable, so that
The output shaft of electric cylinders 10 is directed at first 8 button of pipettor to be checked；
(2) it is worked by PC control electric cylinders 10, so that the pressing of the output end with pressure sensor 11 is calibrated liquid relief
Device button, until being pressed into complete halt；During the acquisition pressing of pressure sensor 11, the pressure of button press, and
Pressure value being fed back into host computer, as shown in Figure 5, wherein initial pressure is fixation pressure caused by pressure sensor is installed,
First stage is when sensor contacts pipettor button until being pressed into the first halt, and second stage is to press from the first halt
It is depressed into complete halt.Host computer is calibrated 8 button of pipettor and presses in the first pressing stage and second according to pressure value, acquisition
Pressure value F1, F2 in stage, to guarantee accuracy, the maximum pressure value that pressure value F1 took for the first pressing stage measured；F2 takes
The maximum pressure value that two pressing stages measured；The first pressing stage is to start to be pressed into the stage for being depressed into the first halt；
Second pressing stage was the stage that the first halt is depressed into complete halt, and PC control electric cylinders 10 reset.
(3) PC control electric cylinders 10 work, when the collected contact pressure value of pressure sensor 11 is greater than F1, on
Position machine control electric cylinders 10 stop.The electronic slide unit 3 of PC control, so that the vertical dropping distance X1 of straight line slide unit 4, is calibrated at this time
The suction nozzle of pipettor 2 to 3mm under 13 liquid level of container at.PC control electric cylinders 10 reset, and draw liquid into suction nozzle, wait 2s
The electronic slide unit 3 of PC control resets afterwards.
(4) host computer obtains initial mass value m1 by electronic balance 12.
(5) the electronic slide unit 3 of PC control, so that the vertical dropping distance X2 of straight line slide unit 4, is calibrated pipettor at this time
Suction nozzle is at 13 ullage 2 to 3mm of container.10 output shaft of PC control electric cylinders is mobile, when pressure sensor 11 collects
Contact pressure value when being greater than F1, PC control electric cylinders 10 stop, and wait 2s, and 10 output shaft of PC control electric cylinders is mobile,
When the collected contact pressure value of pressure sensor 11 is more than or equal to F2, PC control electric cylinders 10 stop.Pipettor at this time
By the fluid removal in suction nozzle.
(6) host computer obtains final mass value m2 by electronic balance 12.
(7) host computer obtains liquid actual temperature t in container by temperature sensor 14, obtains corresponding under fluid temperature
Calibration factor related coefficient K (t), obtain actual volume v '=K (t) (m2-m1).
(8) it is resetted by the electronic slide unit 3 of PC control, electric cylinders 10.
(9) k measurement is carried out according to step 3~8, obtains the volume number that first pipettor is measured at range V1
According to for v111 '~v11k '；
(10) host computer successively controls electrical turntable 6 and rotates forward 90 °, carries out according to step 2-9 to remaining three pipettor
Test, obtains its volume data of excess-three branch pipettor at range V1 respectively；
(11) range for adjusting 4 pipettors obtains body of 4 pipettors at range V2, V3 according to step 2-10
Volume data；
(12) according to measurement volume and range, the capacity relative error and capacity repeatability of pipettor are obtained.Wherein, i-th
Capacity relative error of the branch pipettor at range Vj are as follows:
Wherein
v′ijnFor n-th experimental data of i-th pipettor at range Vj.
Capacity repeatability of i-th pipettor at range Vj are as follows:
Wherein
Wherein, i indicates the serial number for being calibrated pipettor, and j indicates the range serial number being calibrated, and k expression is tested.
According to the method described above, the pipettor that the range of commercially available four brands is 100-1000ul is calibrated, 15
The capacity relative error and capacity repeatability of 4 pipettors are obtained in minute, it is as shown in the table respectively.
Claims (3)
1. a kind of batch calibrating installation of hand-held pipettor capacity, which is characterized in that including host computer, electric cylinders (10), electronic
Turntable (6), cross bracket (16), horizontal bracket (5), frame (1), and it is vertically installed at the electronic slide unit in frame (1) side
(3), relatively another side rails (7) are mounted on, being mounted on frame (1) bottom surface balance (12) and the container that is placed on balance
(13), there is liquid and temperature sensor (14) in container (13)；It is equipped with sliding block (15) on the guide rail (7), it is described electronic
Slide unit (3) is made of straight line slide unit (4) and drive motor, and one end of the horizontal bracket (5) and the straight line slide unit (4) are fixed
Connection, the other end are connect with sliding block (15)；The electric cylinders (10) are mounted on horizontal bracket (5), and output shaft passes through from top to bottom
Horizontal bracket (5), end installation by adhering have pressure sensor (11)；The electrical turntable (6) is mounted on horizontal bracket (5)
Bottom surface, the cross bracket (16) are connected with the output shaft of electrical turntable (6), four branches ends of cross bracket (16)
Fixture with fixed pipettor；The length of the branch is equal to the level between electric cylinders (10) and electrical turntable (6) output shaft
Distance；The output shaft of the electric cylinders (10) and the container (13) are located on same vertical axis；The electric cylinders (10), electric rotating
Platform (6), electronic slide unit (3), pressure sensor (11), temperature sensor (14) and balance (12) are connected with host computer respectively.
2. a kind of calibration method of claim 1 described device, which comprises the following steps:
(1) 4 pipettors to be checked are fixed on cross bracket (16) by fixture, and set the range V1 of pipettor；
By the electronic slide unit of PC control (3), electrical turntable (6), electric cylinders (10) integral reset, electrical turntable (6) rotational angle A1,
So that the output shaft of electric cylinders (10) is directed at first pipettor (8) button to be checked；
(2) it is worked by PC control electric cylinders (10), so that the pressing of the output end with pressure sensor (11) is calibrated liquid relief
Device button, until being pressed into complete halt；During pressure sensor (11) acquisition pressing, the pressure of button press,
And pressure value is fed back into host computer；According to pressure value, acquisition is calibrated pipettor (8) button and presses the stage first host computer
With pressure value F1, F2 in the second pressing stage, the first pressing stage is to start to be pressed into the stage for being depressed into the first halt；
Second pressing stage was the stage that the first halt is depressed into complete halt, and PC control electric cylinders (10) reset；
(3) PC control electric cylinders (10) work, upper when the collected contact pressure value of pressure sensor (11) is greater than F1
Machine, which controls electric cylinders (10), to be stopped；The electronic slide unit of PC control (3) so that the vertical dropping distance X1 of straight line slide unit (4), at this time by
Calibrate pipettor suction nozzle 2 to 3mm under container (13) liquid level at；PC control electric cylinders (10) reset, and draw liquid into suction
Head waits the electronic slide unit of PC control (3) after 2s to reset；
(4) host computer obtains initial mass value m1 by balance (12)；
(5) the electronic slide unit of PC control (3), so that the vertical dropping distance X2 of straight line slide unit (4), is calibrated pipettor at this time
Suction nozzle is at container (13) ullage 2 to 3mm；PC control electric cylinders (10) output shaft is mobile, when pressure sensor (11)
When collected contact pressure value is greater than F1, PC control electric cylinders (10) stop, and wait 2s, and PC control electric cylinders (10) are defeated
Shaft is mobile, and when the collected contact pressure value of pressure sensor (11) is more than or equal to F2, PC control electric cylinders (10) are stopped
Only；Pipettor is by the fluid removal in suction nozzle at this time；
(6) host computer obtains final mass value m2 by balance (12)；
(7) host computer obtains liquid actual temperature t in container by temperature sensor (14), obtains corresponding under fluid temperature
Calibration factor related coefficient K (t) obtains actual volume v '=K (t) (m2-m1)；
(8) it is resetted by the electronic slide unit of PC control (3), electric cylinders (10)；
(9) k measurement is carried out according to step 3 ~ 8, obtains the volume data that first pipettor is measured at range V1, is
v111' ~ v11k'；
(10) host computer successively controls electrical turntable (6) and rotates forward 90 °, surveys according to step 2-9 to remaining three pipettor
Examination, obtains its volume data of excess-three branch pipettor at range V1 respectively；
(11) range for adjusting 4 pipettors obtains volume number of 4 pipettors at range V2, V3 according to step 2-10
According to；
(12) according to measurement volume and range, the capacity relative error and capacity repeatability of pipettor are obtained；Wherein, i-th shifting
Capacity relative error of the liquid device at range Vj are as follows:
, wherein,
For n-th experimental data of i-th pipettor at range Vj；
Capacity repeatability of i-th pipettor at range Vj are as follows:
, wherein,
Wherein, i indicates the serial number for being calibrated pipettor, and j indicates the range serial number being calibrated, and k indicates the number tested.
3. according to the method described in claim 2, it is characterized in that, the first pressing staged pressure value F1 is the first pressing rank
The maximum pressure value that section measures；Second pressing staged pressure value F2 is the maximum pressure value measured in the second pressing stage.
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| CN201710318255.6A CN107115905B (en) | 2017-05-08 | 2017-05-08 | A kind of the batch calibrating installation and calibration method of hand-held pipettor capacity |
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| CN201710318255.6A CN107115905B (en) | 2017-05-08 | 2017-05-08 | A kind of the batch calibrating installation and calibration method of hand-held pipettor capacity |
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| CN107115905B true CN107115905B (en) | 2019-04-09 |
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Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107917747A (en) * | 2017-11-15 | 2018-04-17 | 中国计量大学 | A kind of hand-held capacity calibration device of pipettor and its calibration method |
| CN108414057B (en) * | 2018-02-08 | 2024-07-02 | 水利部交通运输部国家能源局南京水利科学研究院 | Non-contact type water level gauge detection and calibration device |
| CN108489576A (en) * | 2018-07-09 | 2018-09-04 | 中国计量大学 | A kind of calibrating installation and calibration method of small volumetric(al) standards device |
| PL3911442T3 (en) * | 2019-01-17 | 2024-08-05 | Dan Yehoshoa SHAHAR | Pipettor calibration system devices and methods thereof |
| CN110075940A (en) * | 2019-06-03 | 2019-08-02 | 熙迈(上海)检测技术服务有限公司 | A kind of pipettor calibrator (-ter) unit |
| JP2022535424A (en) | 2019-06-05 | 2022-08-08 | コーニング インコーポレイテッド | portable pipettor stand |
| CN112061799B (en) * | 2020-09-29 | 2021-09-07 | 闽江学院 | Nylon spinning cake stacking device |
| CN116754053B (en) * | 2023-08-16 | 2023-11-14 | 中国测试技术研究院 | Trace instrument and dynamic ultra-micro pipetting device calibration method |
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| CN102974415A (en) * | 2012-11-14 | 2013-03-20 | 宁波拓普森科学仪器有限公司 | Capacity calibration device of pipettor |
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| WO2008128864A1 (en) * | 2007-04-20 | 2008-10-30 | Qiagen Instruments Ag | Pipette device with weight measurement, together with method |
| CN102974415A (en) * | 2012-11-14 | 2013-03-20 | 宁波拓普森科学仪器有限公司 | Capacity calibration device of pipettor |
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