CN107114031A - A kind of method of Nepal open country paulownia seed endosperm vigor after raising Cord blood - Google Patents
A kind of method of Nepal open country paulownia seed endosperm vigor after raising Cord blood Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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Abstract
A kind of method of Nepal open country paulownia seed endosperm vigor after raising Cord blood, including：Closed container by 18 DEG C of dry seeds to 20 DEG C of Cord bloods together with storage seed takes out；It is 15 DEG C, places 24 hours in the environment of relative humidity 15% in temperature, takes out seed；Seed is placed in the incubator that temperature is 45 DEG C, heat treatment is taken out after 6 hours.Seed is returned to after room temperature and is positioned on 1% agar medium, is placed between 20 DEG C of cultures 24 hours；Seed after processing, which is cut, to break in the seed coat and endosperm, is soaked in 1% triphenyltetrazolium chloride (TTC) solution, is positioned over 30 DEG C of dark conditions lower 48 hours；Take out the seed after immersion and cut observation endosperm staining conditions, have great-hearted endosperm and show uniform red.This method significantly improves the endosperm vigor for the wild paulownia seed of Nepal that different time is stored under different sources, low temperature.Solve the problem of endosperm vigor is reduced after the wild paulownia seed low-temperature preservation of Nepal.
Description
Technical field
The invention belongs to Preservation of plant germplasin technical field, in particular it relates to which a kind of improve (- 18 DEG C to -20 of low temperature
DEG C) preserve after the wild paulownia seed endosperm vigor of Nepal method.
Background technology
Nepal open country paulownia (Mallotus nepalensis M ü ll.Arg.) is the wild paulownia of Euphorbiaceae (Euphorbiaceae)
Belong to (Mallotus Lour.) machaka or dungarunga seeds.Be distributed in 1700 to 2500 meters of Tibet Yunnan height above sea level hillside or
In cheuch, foreign countries are mainly distributed on Bhutan, India northeast, Burma and Nepal, and studies in China report is less, and foreign countries have been reported that it
Extract has the gentle effect for suppressing nervous centralis.Have no the research report in terms of the wild paulownia seed storage of Nepal.
Low temperature (- 18 DEG C to -20 DEG C) preservation can greatly extend its life-span after orthodox seed is dried, and this is in the world
On built consensus, and multiple crops and Seeds of Wild Plants storehouse are established based on this theory；But some plants
The vigor of seed endosperm after refrigeration can change, and endosperm is also a kind of important germ plasm resource, and be natural three
Times body breeding material.Therefore improve and improve the vigor of seed endosperm after deepfreeze for efficiently preserving and rationally utilizing for a long time
Plant germplasm resource has great significance.
Mainly there are sprouting and triphenyltetrazolium chloride (TTC) decoration method to the method that seed carries out viability examination at present
(being also TZ decoration methods).Present invention discover that the wild paulownia seed of Nepal can hardly be sprouted after refrigeration in practice is preserved, it is entered
Found after row TTC dyeing, embryo can be dyed to red, and endosperm can not be dyed；Therefore it is probably due to endosperm that the present invention, which judges,
Vigor declines caused by certain change occurs in cold storage procedure, and then influences the overall activity and germination rate of seed；For this
The method of the present invention, the vigor to improve endosperm are invented.The evaluation method of endosperm vigor is aided with kind based on dyeing with TTC
Sub- germination rate is reference, to ensure the preciseness and confidence level of experimental result.
The content of the invention
It is an object of the invention to for Buddhist nun in the prior art after (- 18 DEG C to -20 DEG C) preservation a period of times of low temperature
The technical barrier that your wild paulownia seed endosperm vigor of pool declines to a great extent or even almost completely lost improves low there is provided a kind of simple and effective
The method of the wild paulownia seed endosperm vigor of Nepal after temperature is preserved.For realize the permanently effective preservation of the species, particularly endosperm this
Plant the permanently effective preservation of natural triploid germ plasm resource and efficiently utilize and technical support is provided.
In order to realize the above-mentioned purpose of the present invention, the invention provides following technical scheme：
The method of Nepal open country paulownia seed endosperm vigor, comprises the following steps after a kind of raising Cord blood：
(1) seed after will be stored refrigerated at -18 DEG C to -20 DEG C takes out together with its closed container, is 15 DEG C, phase in temperature
Risen again within 24 hours to being placed in the environment of humidity 15%；
(2) seed after rising again is taken out from closed container, be placed in the incubator that temperature is 45 DEG C, heat treatment 6
Taken out after hour；
(3) seed after step (2) processing is taken out, 1% agar training is positioned over after seed temperature returns to room temperature
Support on base, be positioned between 20 DEG C of cultures 24 hours；
(4) seed after above-mentioned steps (3) processing is cut and broken in the seed coat and endosperm；
(5) seed after above-mentioned steps (4) processing is soaked in 1% triphenyltetrazolium chloride (TTC) solution, put
It is placed in 30 DEG C of dark conditions lower 48 hours；
(6) seed after above-mentioned steps (5) processing is cut into observation endosperm staining conditions, has great-hearted endosperm and show
Even red.
According to the method for Nepal open country paulownia seed endosperm vigor after a kind of described raising Cord blood, wherein the step
(1) in -18 DEG C to -20 DEG C stored refrigerated seeds it is the seed that gathers mature and plump, seed is first placed in temperature for 15 DEG C, phase
To until the equilibrium relative humidity (ERH) of seed reaches 15%, (now the water content of seed is usually less than in the environment of humidity 15%
10%), then seed is put into closed container again, -18 DEG C to -20 DEG C are stored in.
The present invention is a kind of method for improving the wild paulownia seed endosperm vigor of Nepal after Cord blood, more specifically step
It is：
Gather the seed of mature and plump, by seed be placed in temperature for 15 DEG C, in the environment of relative humidity 15%, to seed
When equilibrium relative humidity (ERH) reaches 15%, seed is put into closed container, is stored in -18 DEG C to -20 DEG C；During one section of Seed storage
Between after, the closed container that will be equipped with seed takes out, and is 15 DEG C, places in the environment of relative humidity 15% 24 hours and return in temperature
Temperature；Then seed is taken out from container, is placed in the incubator that incubator temperature is 45 DEG C, heat treatment is taken out after 6 hours,
It is positioned on 1% agar medium, is positioned between 20 DEG C of cultures 24 hours after seed temperature returns to room temperature；After processing
Seed, which is cut, to break in the seed coat and endosperm, is soaked in 1% triphenyltetrazolium chloride (TTC) solution, is positioned over 30 DEG C of dark conditions
Lower 48 hours；Take out seed and cut observation endosperm staining conditions, have great-hearted endosperm and show uniform red.
Embodiment
The essentiality content of the present invention is further illustrated with the embodiment of the present invention below, but this hair is not limited with this
It is bright.
Embodiment 1：
1. material and method：
1.1 research material：
In October, 2014, the wild paulownia of the ripe fresh Nepal of Xin Tian villages of Mt. Huang in Anhui Huizhou District Fu Xi townshiies collection
(Mallotus nepalensis) seed.Transport Institute of Zoology southwestern wildlife germplasm resource bank seed in Kunming after collection back
Collection.
1.2 research method：
Rising again after 1.2.1 drying refrigeration and refrigerating：The seed that 1.1 steps are collected is placed in 15 DEG C, and 15% is relatively wet
Dried in the drying room of degree.The Hygrolab C1unit relative humidity analyzer knots periodically produced with Rotronic Ltd. companies
The Rotronic HC 2-AW probes of the company of contract one production are measured to seed relative humidity, and ready to balance relative humidity reaches
It is fitted into after 15% in closed container.The closed container that September in 2015 will be equipped with seed on the 14th is put into -18 DEG C to -20 DEG C refrigerations.
Chilling treatment will be equipped with seed closed container after 1 year takes out.It is put into 24 hours in 15 DEG C, the drying room of 15% relative humidity and returns
Opened after temperature and fill seed-bearing container, it is random to take out seed needed for experiment.
1.2.2 experimental design：Seed is divided into 2 groups of processing, respectively heat treatment group after control group and refrigeration；Heat treatment temperature
Degree is set to 45,60 and 70 DEG C, the time is 6 hours.Each 3 repetitions of processing, each repeat 20 seeds.
1.2.3 heat treatment method：Seed is put into incubator, incubator temperature is set as 45,60 and 70 DEG C.Handle 6 small
When after random take out 60 seeds and be used to detect endosperm vigor.For 45 and 60 DEG C of seed, sprouted while taking out 60 at random and doing
Hair experiment, influence of the heat treatment to endosperm vigor is proved with germination rate.
1.2.4TTC dyeing：Seed temperature after to be heated returns to room temperature, and seed is positioned over to 1% agar culture
On base, it is positioned between 20 DEG C of cultures 24 hours；Take out seed to cut and breaks in the seed coat and endosperm, and seed is soaked in 1% chlorination three
In phenyl tetrazole (TTC) solution, it is positioned over 30 DEG C of dark conditions lower 48 hours, seed is taken out after the completion of dyeing, blotting paper is used
Surface residual solution is blotted, seed is cut, endosperm staining conditions are observed, has great-hearted endosperm and shows uniform red.
1.2.5 sprout：Sprouting experiment is carried out to the seed of 45 and 60 DEG C of heat treatment 6 hours after control group and refrigeration, to help
Demonstrate,prove endosperm vigour changes.Seed is seeded on 1% agar medium containing 200 milligrams per liter of gibberellin, culture medium is filled
Entering in transparent valve bag prevents moisture loss, is subsequently placed in 20 DEG C of illumination box, and intensity of illumination is 1000 luxs,
Photoperiod is 12 hours illumination/12 hour dark.Checked and once sprout at interval of 2-3 days, radicle extends over 5mm and thinks kind
Son is sprouted, and germination seed is taken out, and record sprouting quantity；Terminate experiment without sprouting within continuous 2 weeks after experiment starts 4 weeks.
1.2.6 data analysis：Endosperm colored proportion data are analyzed using SPSS16.0 softwares, using One-Way
ANOVA carries out variance analysis, and does Multiple range test to the colored proportion of different disposal time with S-N-K methods.
2. result
2.1 are heat-treated the influence to picking up from the wild paulownia seed endosperm vigor of Nepal after Mt. Huang in Anhui refrigeration：
Table 1 is heat-treated the shadow to Mt. Huang in Anhui Nepal open country paulownia seed endosperm rate of dyeing (%) and germination rate (%) after refrigeration
Ring
Note：Numeral is average value ± standard error, and different letters represent that the rate of dyeing between different disposal has significant difference
As can be seen from Table 1, seed endosperm rate of dyeing not thermally treated after refrigeration 1 year at -20 DEG C only has 4.2%, right
The seed germination rate answered is 0；Endosperm colored proportion is to start rise after 45 DEG C of processing 6 hours, and germination rate also brings up to 28.3%,
60 DEG C are risen to temperature, endosperm vigor and sprouting are showed no notable rising, 70 DEG C of endosperm vigor compared with 45 and 60 DEG C
It is decreased significantly.
Consider effect and energy consumption, selected 45 DEG C of the present invention is handled 6 hours, can effectively improve endosperm vigor, and
Using the method for 45 DEG C of processing 6 hours in following examples.
Embodiment 2：
1. material and method：
1.1 research material：
The ripe wild paulownia (Mallotus of fresh Nepal of Wenshan Prefecture of the Yunnan Province Wenshan County collection on the 4th of September in 2012
Nepalensis) seed.Transport Institute of Zoology southwestern wildlife germplasm resource bank Seed Deposit center in Kunming after collection back.
1.2 research method：
Rising again after 1.2.1 drying refrigeration and refrigerating：1.1 seeds collected are placed in 15 DEG C, 15% relative humidity
Dried in drying room.The Hygrolab C1unit relative humidity analyzer periodically produced with Rotronic Ltd. companies combines same
The Rotronic HC 2-AW probes of one company production are measured to seed relative humidity, and ready to balance relative humidity reaches 15%
It is fitted into afterwards in closed container.The closed container that August in 2013 will be equipped with seed on the 6th is put into -18 DEG C to -20 DEG C refrigerations.Refrigeration 3
The container that seed is will be equipped with after 2 months years takes out.It is put into after rising again within 24 hours in 15 DEG C, the drying room of 15% relative humidity and opens
Seed-bearing container is filled, it is random to take out seed needed for experiment.
1.2.2 experimental design：Seed is divided into 2 groups of processing, respectively control group and heat treatment group；Heat treatment temperature is set
45 DEG C, handle 6 hours.Each 3 repetitions of processing, each repeat 20 seeds.
1.2.3 heat treatment method：Seed is put into incubator, incubator temperature is set as 45 DEG C.Processing is taken out after 6 hours
60 seeds are used for TTC Coloration experiments.
1.2.4TTC dyeing：Seed temperature after to be heated returns to room temperature, and seed is positioned over to 1% agar culture
On base, it is positioned between 20 DEG C of cultures 24 hours；Take out seed to cut and breaks in the seed coat and endosperm, and seed is soaked in 1% chlorination three
In phenyl tetrazole (TTC) solution, it is positioned over 30 DEG C of dark conditions lower 48 hours, seed is taken out after the completion of dyeing, blotting paper is used
Surface residual solution is blotted, seed is cut, endosperm staining conditions are observed, has great-hearted endosperm and shows uniform red.
1.2.6 data analysis：Endosperm rate of dyeing data are analyzed using SPSS16.0 softwares, using Paired-
Samples T Test carry out variance analysis.
2. result
Influence of 2.1 heat treatments to Yunnan mountain of papers Nepal open country paulownia seed endosperm vigor after refrigeration.
Table 2 is heat-treated the influence to Yunnan mountain of papers Nepal open country paulownia seed endosperm rate of dyeing (%) after refrigeration
Note：Numeral is average value ± standard error, and different letters represent that the rate of dyeing between different disposal has significant difference
As can be seen from Table 2, the wild paulownia seed of the Nepal of refrigeration 2 months 3 years, not thermally treated seed embryo at -20 DEG C
Newborn rate of dyeing is 0；After being heat-treated through 45 DEG C, endosperm rate of dyeing brings up to 42.4%, illustrate to be heat-treated effectively improve -20 DEG C cold
Seed endosperm vigor behind Tibetan.
Embodiment 3：
1. material and method：
1.1 research material：
On October 08th, 2009, the wild paulownia (Mallotus of the ripe fresh Nepal of Yunnan Pu'er Zhen Yuan Jiu Jia townshiies collection
Nepalensis) seed.Transport Institute of Zoology southwestern wildlife germplasm resource bank Seed Deposit center in Kunming after collection back.
1.2 research method：
Rising again after 1.2.1 drying refrigeration and refrigerating：1.1 seeds collected are placed in 15 DEG C, 15% relative humidity
Dried in drying room.The Hygrolab C1unit relative humidity analyzer periodically produced with Rotronic Ltd. companies combines same
The Rotronic HC 2-AW probes of one company production are measured to seed relative humidity, and ready to balance relative humidity reaches 15%
It is fitted into afterwards in closed container.The closed container that in September, 2011 will be equipped with seed is put into -18 DEG C to -20 DEG C refrigerations.Refrigeration 5 years
The container that will be equipped with seed afterwards takes out.It is put into after rising again within 24 hours in 15 DEG C, the drying room of 15% relative humidity to open to be equipped with and plants
The container of son, the random seed for taking out respective numbers is needed according to experiment.
1.2.2 experimental design：Seed is divided into 2 groups of processing, respectively control group and heat treatment group；Heat treatment temperature is set
45 DEG C, handle 6 hours.Each 3 repetitions of processing, each repeat 20 seeds.
1.2.3 heat treatment method：Seed is put into incubator, incubator temperature is set as 45 DEG C.Processing is taken out after 6 hours
60 seeds are used for TTC Coloration experiments.
1.2.4TTC dyeing：Seed temperature after to be heated returns to room temperature, and seed is positioned over to 1% agar culture
On base, it is positioned between 20 DEG C of cultures 24 hours；Take out seed to cut and breaks in the seed coat and endosperm, and seed is soaked in 1% chlorination three
In phenyl tetrazole (TTC) solution, it is positioned over 30 DEG C of dark conditions lower 48 hours, seed is taken out after the completion of dyeing, blotting paper is used
Surface residual solution is blotted, seed is cut, endosperm staining conditions are observed, has great-hearted endosperm and shows uniform red.
1.2.6 data analysis：Endosperm rate of dyeing data are analyzed using SPSS16.0 softwares, using Paired-
Samples T Test carry out variance analysis.
2. result is with discussing
Influence of 2.1 heat treatments to Yunnan Pu'er Nepal open country paulownia seed endosperm rate of dyeing after refrigeration.
Table 4 is heat-treated the influence to Yunnan Pu'er Nepal open country paulownia seed endosperm rate of dyeing (%) after refrigeration
Note：Numeral is average value ± standard error, and different letters represent that the rate of dyeing between different disposal has significant difference
As can be seen from Table 4, the wild paulownia seed of the Nepal of refrigeration 5 years at -20 DEG C, not thermally treated seed endosperm dyeing
Rate is 0；After being heat-treated through 45 DEG C, endosperm rate of dyeing has reached 48.1%, and the vigor of showing is significantly improved.
3rd, the positive effect of the present invention is as follows：
Compared with prior art, invention significantly improves different sources (Anhui and Yunnan), (- 18 DEG C of low temperature after drying
To -20 DEG C) under storage different time (1 year, 2 months 3 years and 5 years) the wild paulownia seed endosperm of Nepal vigor.Preferably solve
The problem of endosperm vigor is reduced after Jue Liao Nepal open country paulownia seed low-temperature preservation.And processing method is simple and easy to do, 45 DEG C handle 6
There is remarkable result hour, and without any dangerous and toxic side effect.The present invention is advantageously implemented the species in seed bank bar
Long-term preservation under part, and the permanently effective preservation of this natural triploid germ plasm resource and reasonable utilize provide for endosperm
Technical support.
Claims (3)
1. a kind of method for improving the wild paulownia seed endosperm vigor of Nepal after Cord blood, it is characterised in that：This method is included such as
Lower step：
(1) closed container by the dry seed after -18 DEG C to -20 DEG C Cord bloods together with stored seed takes out, in temperature
For 15 DEG C, place and rise again in the environment of relative humidity 15% for 24 hours；
(2) seed after rising again is placed in the incubator that temperature is 45 DEG C, heat treatment is taken out after 6 hours；
(3) seed after step (2) processing is taken out, 1% agar medium is positioned over after seed temperature returns to room temperature
On, it is positioned between 20 DEG C of cultures 24 hours；
(4) seed after above-mentioned steps (3) processing is cut and broken in the seed coat and endosperm；
(5) seed after above-mentioned steps (4) processing is soaked in 1% triphenyltetrazolium chloride solution, is positioned over 30 DEG C
Lower 48 hours of dark condition；
(6) seed after above-mentioned steps (5) processing is cut into observation endosperm staining conditions, has great-hearted endosperm and show uniformly
It is red.
2. a kind of method for improving the wild paulownia seed endosperm vigor of Nepal after Cord blood according to claim 1, it is special
Levy and be：The step (1) is the seed for gathering mature and plump in -18 DEG C to -20 DEG C stored refrigerated seeds, first puts seed
In temperature be 15 DEG C, in the environment of relative humidity 15% until the equilibrium relative humidity (ERH) of seed reaches 15%, seed moisture content is low
It is put into when 10%, then by seed in closed container, is stored in -18 DEG C to -20 DEG C.
3. a kind of method for improving the wild paulownia seed endosperm vigor of Nepal after Cord blood according to claim 1, it is special
Levy and be：This method is the seed for gathering mature and plump, by seed be placed in temperature for 15 DEG C, in the environment of relative humidity 15%,
When reaching 15% to the equilibrium relative humidity (ERH) of seed, seed is put into closed container, is stored in -18 DEG C to -20 DEG C；According to need
The closed container that will be equipped with seed over time takes out, and is 15 DEG C, places in the environment of relative humidity 15% in temperature
Rise again within 24 hours；Then seed is taken out from container, is placed in the incubator that temperature is 45 DEG C, will after being heat-treated 6 hours
Seed after processing takes out, and is positioned over after seed temperature returns to room temperature on 1% agar medium, is positioned over 20 DEG C of cultures
Between 24 hours；Seed after processing, which is cut, to break in the seed coat and endosperm, is soaked in 1% triphenyltetrazolium chloride solution, is positioned over
Lower 48 hours of 30 DEG C of dark conditions；Take out seed and cut observation endosperm staining conditions, have great-hearted endosperm and show uniform red
Color.
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| CN201710330337.2A CN107114031B (en) | 2017-05-11 | 2017-05-11 | Method for improving endosperm activity of mallotus nepalensis seeds after low-temperature storage |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH01168601A (en) * | 1987-12-24 | 1989-07-04 | Gakken Co Ltd | Preservation of seed of japanese horse radish |
| CN106508182A (en) * | 2016-12-16 | 2017-03-22 | 界首市兴邦家庭农场 | Handling method of accelerating germination of seed of fructus lycii |
| CN106508183A (en) * | 2016-12-21 | 2017-03-22 | 阜阳市颍泉区玉寿种植专业合作社 | Germination accelerating method for fructus crataegi seeds |
-
2017
- 2017-05-11 CN CN201710330337.2A patent/CN107114031B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH01168601A (en) * | 1987-12-24 | 1989-07-04 | Gakken Co Ltd | Preservation of seed of japanese horse radish |
| CN106508182A (en) * | 2016-12-16 | 2017-03-22 | 界首市兴邦家庭农场 | Handling method of accelerating germination of seed of fructus lycii |
| CN106508183A (en) * | 2016-12-21 | 2017-03-22 | 阜阳市颍泉区玉寿种植专业合作社 | Germination accelerating method for fructus crataegi seeds |
Non-Patent Citations (5)
| Title |
|---|
| 侯冬花等: "种子休眠与休眠解除的研究进展", 《新疆农业科学》 * |
| 张百俊: "干热处理对番茄种子活力的影响", 《长江蔬菜》 * |
| 蒋先明: "《蔬菜栽培生理学》", 31 October 1996, 中国农业出版社 * |
| 颜启传: "《种子学》", 30 April 2004, 中国农业出版社 * |
| 龙文靖等: "不同温度和时间干热处理对高粱种子萌发的影响", 《大麦与谷类科学》 * |
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