CN107099461B - Biological cell culture ware strorage device - Google Patents
Biological cell culture ware strorage device Download PDFInfo
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- CN107099461B CN107099461B CN201710450178.XA CN201710450178A CN107099461B CN 107099461 B CN107099461 B CN 107099461B CN 201710450178 A CN201710450178 A CN 201710450178A CN 107099461 B CN107099461 B CN 107099461B
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- culture dish
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- circulation groove
- impeller
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/22—Heat exchange systems, e.g. heat jackets or outer envelopes in contact with the bioreactor walls
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- Bioinformatics & Cheminformatics (AREA)
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- General Health & Medical Sciences (AREA)
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Abstract
The invention discloses a biological cell culture dish storage device which comprises a storage rack, wherein the storage rack is provided with two culture dish storage grooves, the two culture dish storage grooves are communicated with each other, one end, far away from each other, of each culture dish storage groove is respectively provided with a first circulation groove and a second circulation groove, the first circulation groove and the second circulation groove are both communicated with the culture dish storage grooves, a first heating element and a first impeller are arranged in the first circulation groove, a second heating element and a second impeller are arranged in the second circulation groove, the first impeller is used for blowing fluid in the first circulation groove into the culture dish storage grooves, and the second impeller is used for blowing fluid in the second circulation groove into the culture dish storage grooves so as to enable the fluid to form circulation in the culture dish storage grooves; a culture dish storage platform is arranged in each culture dish storage tank. This strorage device accuse temperature is accurate, and the temperature that two culture dishes are located can be guaranteed unanimously all the time, and the error is little, guarantees that the contrast experiment can go on accurately.
Description
Technical Field
the invention relates to the technical field of biology, in particular to a biological cell culture dish storage device.
background
In the biological relevant culture experiment process, contrast experiments are often needed, the experiments are usually carried out in culture dishes, the contrast experiments are carried out on the premise that the culture conditions of the two culture dishes are guaranteed, such as temperature, humidity and the like are kept consistent, the experiment conclusion obtained under the single variable condition is more reliable, in the prior art, when isothermal culture is carried out, the culture dishes for the two contrast experiments cannot be fully guaranteed to be always under the same temperature condition, the experiment result is obtained with larger errors, meanwhile, the experiment conclusion is also greatly influenced, and therefore, a biological cell culture dish storage device capable of effectively realizing the isothermal contrast experiments is urgently needed.
Disclosure of Invention
The invention aims to provide a biological cell culture dish storage device to solve the technical problem.
In order to achieve the purpose, the invention provides the following technical scheme:
A biological cell culture dish storage device comprises a storage rack, wherein the storage rack is provided with two culture dish storage grooves which are communicated with each other, one end, far away from each other, of each culture dish storage groove is respectively provided with a first circulation groove and a second circulation groove, the first circulation groove and the second circulation groove are both communicated with the culture dish storage grooves, a first heating element and a first impeller are arranged in the first circulation groove, a second heating element and a second impeller are arranged in the second circulation groove, the first impeller is used for blowing fluid in the first circulation groove into the culture dish storage grooves, and the second impeller is used for blowing fluid in the second circulation groove into the culture dish storage grooves so that the fluid forms circulation in the culture dish storage grooves;
Every be provided with the culture dish in the culture dish storage tank and put the thing platform.
preferably, the first circulation groove and the second circulation groove are both U-shaped grooves, the first circulation groove and the second circulation groove correspond in position, and the first impeller and the second impeller are respectively located at staggered positions of the first circulation groove and the second circulation groove.
Preferably, the two culture dish storage tanks are communicated through a communication groove, a separation block is arranged in the communication groove and divides the communication groove into a first communication groove and a second communication groove, and the first communication groove and the second communication groove are communicated with the two culture dish storage tanks respectively.
Preferably, two ends of the separation block respectively extend into the two culture dish storage grooves.
Preferably, the end surfaces of the two ends of the separation block are arc surfaces.
preferably, the culture dish placing table is rotatably arranged in the culture dish placing groove through a bearing, and a cross for bearing the culture dish is arranged on the culture dish placing table.
preferably, the culture dish is put the thing platform outside be provided with the buffering bead in the culture dish storage tank, the buffering bead set up in the diapire of culture dish storage tank.
Preferably, the extending direction of the buffer protruding edge is matched with the output direction of the first impeller and the second impeller.
Preferably, a first isolation block and a second isolation block are respectively arranged in the first circulation groove and the second circulation groove, and both the first isolation block and the second isolation block extend into the adjacent culture dish storage groove.
the invention has the beneficial effects that:
The invention has scientific and reasonable structure, because the fluid circulation is formed in the two culture dish storage tanks, and the two heating parts are respectively positioned at two sides of the two culture dish storage tanks, during the circulation, the fluid heated by the first heating part sequentially reaches the two culture dish storage tanks, the temperature is gradually reduced through the heat exchange with the culture dishes, and the fluid with the reduced temperature is heated by the second heating part to form the circulation. And, because the culture dish of placing in two culture dish storage tanks all can with partly hot-fluid and partly cold fluid contact, the temperature keeps moderate scope all the time, and the accuse temperature is more accurate, and the temperature that two culture dishes are located simultaneously can be guaranteed unanimously all the time, and the error is less, guarantees that the contrast experiment can go on accurately.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings needed to be used in the embodiments will be briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present invention and therefore should not be considered as limiting the scope, and for those skilled in the art, other related drawings can be obtained according to the drawings without inventive efforts.
FIG. 1 is a top view of a biological cell culture dish storage device of the present invention;
FIG. 2 is a schematic structural view of a culture dish platform in the biological cell culture dish storing device of the present invention;
Fig. 3 is an enlarged view of a point a in fig. 1.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
referring to fig. 1 to 3, the present invention provides a technical solution: the utility model provides a biological cell culture dish strorage device, includes storage rack 100, and storage rack 100 is provided with two culture dish storage tank 120, and two culture dish storage tank 120 all are circular, and two culture dish storage tank 120's size is roughly the same.
The two culture dish storage grooves 120 are communicated with each other through the communication groove, the separation block 400 is arranged in the communication groove, the communication groove is separated into a first communication groove 410 and a second communication groove 420 by the separation block 400, and the first communication groove 410 and the second communication groove 420 are communicated with the two culture dish storage grooves 120 respectively.
The end of each culture dish storage groove 120 far away from each other is provided with a first circulation groove 200 and a second circulation groove 300 respectively, namely the first circulation groove 200 is adjacent to one culture dish storage groove 120, the second circulation groove 300 is adjacent to the other culture dish storage groove 120, the first circulation groove 200 and the second circulation groove 300 are positioned at two sides of the two culture dish storage grooves 120, and the two culture dish storage grooves 120 are positioned between the first circulation groove 200 and the second circulation groove 300. Preferably, in this embodiment, the first circulation tank 200 and the second circulation tank 300 are disposed correspondingly, and a central connecting line of the first circulation tank and the second circulation tank passes through the centers of the two culture dish storage tanks 120.
The first circulation tank 200 and the second circulation tank 300 are both communicated with the culture dish storage tank 120, a first heating member 202 and a first impeller 203 are arranged in the first circulation tank 200, the first heating member 202 is used for controlled heating to heat fluid (water) flowing in the culture dish storage tank 120, and the heating temperature of the first heating member 202 is adjustable and controllable. The first impeller 203 is used to blow the fluid heated by the first heating member 202 into the dish storing tank 120 adjacent to the first circulation tank 200.
A second heating member 302 and a second impeller 303 are provided in the second circulation tank 300, and the first heating member 202 is used for controlled heating to heat the fluid (water) flowing in the petri dish storage tank 120, wherein the heating temperature of the second heating member 302 is adjustable and controllable. The second impeller 303 is used to blow the fluid heated by the second heating member 302 into the dish storing tank 120 adjacent to the second circulation tank 300.
The fluid is circulated within the two culture dish storage tanks 120 by the first impeller 203 and the second impeller 303. Specifically, the first circulation tank 200 and the second circulation tank 300 are both "U" shaped tanks, and the first impeller 203 and the second impeller 303 are respectively located at staggered positions of the first circulation tank 200 and the second circulation tank 300. Namely, the two free ends of the two U-shaped grooves correspond to each other, and the first impellers 203 are disposed on both sides of a line connecting the centers of the two petri dish storage grooves 120, and have opposite output directions.
specifically, a first isolation block 201 and a second isolation block 301 are respectively arranged in the first circulation tank 200 and the second circulation tank 300, and the first isolation block 201 and the second isolation block 301 respectively separate the first circulation tank 200 and the second circulation tank 300 into U-shaped grooves. The first and second isolation blocks 201 and 301 extend into the adjacent culture dish storage tanks 120, so that when fluid flows in the culture dish storage tanks 120 and moves to the first or second circulation tanks 200 and 300, the fluid is blocked by the first or second isolation blocks 201 and 301, and then the fluid can enter the first or second circulation tanks 200 and 300 to be heated, and cannot self-circulate in one culture dish storage tank 120.
The first impeller 203 corresponds to the position of the first communicating groove 410, and the second impeller 303 corresponds to the position of the second communicating groove 420. The two ends of the partition block 400 are extended into the two petri dish storage tanks 120, respectively. The advantage of this design is that when fluid flows in the dish storing groove 120 and moves to the communication groove, the fluid is blocked by the partition block 400 and enters the other dish storing groove 120 along the first communication groove 410 or the second communication groove 420, and the fluid does not self-circulate in the other dish storing groove 120. And preferably, as shown in fig. 3, end surfaces of both ends of the spacer 400 are arc-shaped surfaces 401. After the design, a small amount of fluid which does not enter the other culture dish storage groove 120 through the first communicating groove 410 or the second communicating groove 420 can flow along the current annular flow direction, so that large splash is not generated at the position of the separation block 400, and the splash is prevented from splashing into the culture dish to influence the experiment.
A culture dish placing table 101 is provided in each culture dish storage tank 120. The culture dish placing table 101 is rotatably arranged on a fixed shaft in the culture dish placing groove through a bearing 103, and a cross 102 for bearing a culture dish is arranged on the culture dish placing table 101. The cross 102 is provided with anti-slip threads to prevent the petri dish from sliding under the wash of fluid when placed thereon. When the proper fluid flow rate is adjusted, the fluid can circulate along the fixed direction, at the moment, the culture dish placing table 101 can rotate along the fixed shaft under the driving of the fluid, the purpose of shaking the culture dish is achieved, and microorganisms needing to be subjected to shaking culture do not need to be placed into a shaking box for carrying out. When the rotation of the petri dish platform 101 is not required, the flow rate of the fluid is adjusted to be reduced. The fluid flow rate can be adjusted by adjusting the output power of the first impeller 203 and the second impeller 303, and preferably, the output power of the first impeller 203 and the output power of the second impeller 303 are kept the same.
when it is rotatory in order to drive the culture dish when needing culture dish to put thing platform 101, the fluid flow rate need be transferred high some, and the velocity of flow accelerates this moment, and the fluid is when striking culture dish and putting thing platform 101, easily produces the splash, and in this embodiment, the culture dish is put and is provided with buffering bead 111 in the culture dish storing tank 120 of thing platform 101 outside, and buffering bead 111 sets up in the diapire of culture dish storing tank 120. As shown in FIG. 1, the height of the buffer projected ridges 111 is preferably 0.2 to 0.5 cm. And the extending direction of the buffer rib 111 matches with the output direction of the first impeller 203 and the second impeller 303, that is, the buffer rib 111 is used for buffering the flow speed and suppressing the splash, and the extending direction is as follows: the flow direction of the water flow forms an obtuse angle with the buffer rib 111. Preferably, the obtuse angle is 120 ° to 150 °.
The working principle of the biological cell culture dish storage device provided by the embodiment is as follows: when a contrast test is performed, the two culture dishes for the contrast test are respectively placed on the culture dish placing tables 101 in the two culture dish storing grooves 120, a fluid (water) is placed in the culture dish storing grooves 120, the first impeller 203 and the second impeller 303 are started to circulate the fluid, the temperatures of the first heating element 202 and the second heating element 302 are set to be consistent, and the output powers of the first impeller 203 and the second impeller 303 are set to be consistent as required. At the moment, the fluid can form thermal circulation, and the fluid in the circulation is contacted with the two culture dishes, so that the external temperature environments of the two culture dishes are consistent, and the test result is more accurate.
the fluid circulates through the path from the first impeller 203 to the dish storage tank 120 adjacent to the first impeller 203, then to the first connecting groove 410, to another dish storage tank 120, then to the second circulation tank 300, through the second circulation tank 300, into the dish circulation tank adjacent to the second circulation tank 300, then through the second connecting groove 420 into the dish storage tank 120 adjacent to the first circulation tank 200, and then into the first circulation tank 200 to complete one cycle.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (1)
1. The utility model provides a biological cell culture dish strorage device, characterized by, includes storage rack (100), storage rack (100) is provided with two culture dish storing tank (120), two culture dish storing tank (120) communicate each other, and the one end of keeping away from each other of every culture dish storing tank (120) is provided with first circulation groove (200) and second circulation groove (300) respectively, first circulation groove (200), second circulation groove (300) all with culture dish storing tank (120) intercommunication, be provided with first heating member (202) and first impeller (203) in first circulation groove (200), be provided with second heating member (302) and second impeller (303) in second circulation groove (300), first impeller (203) are used for with the fluid in first circulation groove (200) is bloated into culture dish storing tank (120), second impeller (303) are used for with the fluid in the second circulation groove (300) is bloated into culture dish storing tank (120) To circulate fluid within the culture dish holding tank (120);
A culture dish placing table (101) is arranged in each culture dish storage groove (120);
The first circulation groove (200) and the second circulation groove (300) are both U-shaped grooves, the first circulation groove (200) and the second circulation groove (300) are corresponding in position, and the first impeller (203) and the second impeller (303) are respectively positioned at the staggered positions of the first circulation groove (200) and the second circulation groove (300);
The two culture dish storage grooves (120) are communicated through a communication groove, a separating block (400) is arranged in the communication groove, the separating block (400) separates the communication groove into a first communication groove (410) and a second communication groove (420), and the first communication groove (410) and the second communication groove (420) are respectively communicated with the two culture dish storage grooves (120);
Two ends of the separating block (400) respectively extend into the two culture dish storage grooves (120);
the end surfaces of two ends of the separating block (400) are arc-shaped surfaces (401);
The culture dish placing table (101) is rotatably arranged in the culture dish placing groove (120) through a bearing (103), and a cross (102) for bearing a culture dish is arranged on the culture dish placing table (101);
a buffer rib (111) is arranged in the culture dish storage groove (120) on the outer side of the culture dish placing table (101), and the buffer rib (111) is arranged on the bottom wall of the culture dish storage groove (120);
The extending direction of the buffer convex rib (111) is matched with the output directions of the first impeller (203) and the second impeller (303);
The first circulation groove (200) and the second circulation groove (300) are internally provided with a first isolation block (201) and a second isolation block (301) respectively, and the first isolation block (201) and the second isolation block (301) extend into the adjacent culture dish storage groove (120).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710450178.XA CN107099461B (en) | 2017-06-15 | 2017-06-15 | Biological cell culture ware strorage device |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710450178.XA CN107099461B (en) | 2017-06-15 | 2017-06-15 | Biological cell culture ware strorage device |
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| Publication Number | Publication Date |
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| CN107099461A CN107099461A (en) | 2017-08-29 |
| CN107099461B true CN107099461B (en) | 2019-12-10 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201710450178.XA Active CN107099461B (en) | 2017-06-15 | 2017-06-15 | Biological cell culture ware strorage device |
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Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109609372A (en) * | 2018-12-29 | 2019-04-12 | 乐清市足资初生物科技有限公司 | A kind of culture dish storing unit |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201648393U (en) * | 2010-04-14 | 2010-11-24 | 张桂玲 | Culture dish retainer |
| CN202116540U (en) * | 2011-05-13 | 2012-01-18 | 山东轻工业学院 | Culture tank |
| CN204032002U (en) * | 2014-06-18 | 2014-12-24 | 徐州沃尔地农业科技有限公司 | Plant culture dish dispenser |
| CN205035357U (en) * | 2015-10-08 | 2016-02-17 | 乐山师范学院 | Deposit device of culture dish |
| CN106068124A (en) * | 2014-02-28 | 2016-11-02 | Bdbc科学公司 | A kind of system for tissue manipulation |
-
2017
- 2017-06-15 CN CN201710450178.XA patent/CN107099461B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201648393U (en) * | 2010-04-14 | 2010-11-24 | 张桂玲 | Culture dish retainer |
| CN202116540U (en) * | 2011-05-13 | 2012-01-18 | 山东轻工业学院 | Culture tank |
| CN106068124A (en) * | 2014-02-28 | 2016-11-02 | Bdbc科学公司 | A kind of system for tissue manipulation |
| CN204032002U (en) * | 2014-06-18 | 2014-12-24 | 徐州沃尔地农业科技有限公司 | Plant culture dish dispenser |
| CN205035357U (en) * | 2015-10-08 | 2016-02-17 | 乐山师范学院 | Deposit device of culture dish |
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| CN107099461A (en) | 2017-08-29 |
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Effective date of registration: 20191120 Address after: 264000 Room 215, Yuhuangding West Road, Zhifu District, Yantai, Shandong, 14 Applicant after: YANTAI ZHIXING INTELLECTUAL PROPERTY CONSULTING SERVICE Co.,Ltd. Address before: 265300 No. 199 Tengfei Road, Yantai, Shandong, Qixia Applicant before: Yang Cheng |
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Effective date of registration: 20220624 Address after: Room 501, floor 5, building 14, No. 615, Lianhe Road, Chedun Town, Songjiang District, Shanghai 201600 Patentee after: Shanghai lanweisaier Biotechnology Co.,Ltd. Address before: 264000 Room 215, Yuhuangding West Road, Zhifu District, Yantai, Shandong, 14 Patentee before: YANTAI ZHIXING INTELLECTUAL PROPERTY CONSULTING SERVICE Co.,Ltd. |