CN107096580A - A kind of micro-fluidic chip with rotary valve structure - Google Patents
A kind of micro-fluidic chip with rotary valve structure Download PDFInfo
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- CN107096580A CN107096580A CN201710433647.7A CN201710433647A CN107096580A CN 107096580 A CN107096580 A CN 107096580A CN 201710433647 A CN201710433647 A CN 201710433647A CN 107096580 A CN107096580 A CN 107096580A
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- sealant
- supporting layer
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- gland
- substrate
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- 239000000565 sealant Substances 0.000 claims abstract description 76
- 239000000758 substrate Substances 0.000 claims abstract description 62
- 230000008676 import Effects 0.000 claims abstract description 6
- 210000004907 gland Anatomy 0.000 claims description 65
- 229920002725 thermoplastic elastomer Polymers 0.000 claims description 16
- 238000007789 sealing Methods 0.000 claims description 13
- 239000004215 Carbon black (E152) Substances 0.000 claims description 10
- 229920001577 copolymer Polymers 0.000 claims description 10
- 229930195733 hydrocarbon Natural products 0.000 claims description 10
- 150000002430 hydrocarbons Chemical class 0.000 claims description 10
- 239000002861 polymer material Substances 0.000 claims description 8
- 229910001220 stainless steel Inorganic materials 0.000 claims description 7
- 239000010935 stainless steel Substances 0.000 claims description 7
- 239000000126 substance Substances 0.000 claims description 7
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 claims description 6
- 229910052802 copper Inorganic materials 0.000 claims description 6
- 239000010949 copper Substances 0.000 claims description 6
- 239000003292 glue Substances 0.000 claims description 6
- 238000001746 injection moulding Methods 0.000 claims description 6
- 238000003466 welding Methods 0.000 claims description 6
- 229920001971 elastomer Polymers 0.000 claims description 5
- 239000004743 Polypropylene Substances 0.000 claims description 3
- 229920003229 poly(methyl methacrylate) Polymers 0.000 claims description 3
- 239000004926 polymethyl methacrylate Substances 0.000 claims description 3
- -1 polypropylene Polymers 0.000 claims description 3
- 229920001155 polypropylene Polymers 0.000 claims description 3
- 239000004793 Polystyrene Substances 0.000 claims description 2
- 229920000139 polyethylene terephthalate Polymers 0.000 claims description 2
- 229920002223 polystyrene Polymers 0.000 claims description 2
- 150000002148 esters Chemical class 0.000 claims 3
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims 2
- 239000002253 acid Substances 0.000 claims 2
- 229910052799 carbon Inorganic materials 0.000 claims 2
- 239000005020 polyethylene terephthalate Substances 0.000 claims 1
- 238000000034 method Methods 0.000 abstract description 18
- 238000004519 manufacturing process Methods 0.000 abstract description 5
- 239000000463 material Substances 0.000 description 14
- 239000003153 chemical reaction reagent Substances 0.000 description 13
- 230000006872 improvement Effects 0.000 description 11
- 239000004425 Makrolon Substances 0.000 description 9
- 229920000515 polycarbonate Polymers 0.000 description 9
- 238000005516 engineering process Methods 0.000 description 8
- 238000005260 corrosion Methods 0.000 description 7
- 230000007797 corrosion Effects 0.000 description 7
- 238000010276 construction Methods 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 230000008901 benefit Effects 0.000 description 4
- 238000006243 chemical reaction Methods 0.000 description 4
- 238000013461 design Methods 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 description 3
- 238000001514 detection method Methods 0.000 description 3
- PPBRXRYQALVLMV-UHFFFAOYSA-N Styrene Chemical compound C=CC1=CC=CC=C1 PPBRXRYQALVLMV-UHFFFAOYSA-N 0.000 description 2
- 230000003321 amplification Effects 0.000 description 2
- 238000010923 batch production Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 230000006835 compression Effects 0.000 description 2
- 238000007906 compression Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- BDAGIHXWWSANSR-UHFFFAOYSA-N methanoic acid Natural products OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 description 2
- 238000003199 nucleic acid amplification method Methods 0.000 description 2
- 150000007523 nucleic acids Chemical class 0.000 description 2
- 102000039446 nucleic acids Human genes 0.000 description 2
- 108020004707 nucleic acids Proteins 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 229920001169 thermoplastic Polymers 0.000 description 2
- 239000004416 thermosoftening plastic Substances 0.000 description 2
- OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 description 1
- 238000004026 adhesive bonding Methods 0.000 description 1
- 238000001311 chemical methods and process Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
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- 238000011161 development Methods 0.000 description 1
- 150000005690 diesters Chemical class 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 239000000686 essence Substances 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000002360 explosive Substances 0.000 description 1
- 235000019253 formic acid Nutrition 0.000 description 1
- 238000007306 functionalization reaction Methods 0.000 description 1
- 230000028993 immune response Effects 0.000 description 1
- 238000010030 laminating Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000000465 moulding Methods 0.000 description 1
- 238000002203 pretreatment Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000001039 wet etching Methods 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/06—Valves, specific forms thereof
- B01L2400/0633—Valves, specific forms thereof with moving parts
- B01L2400/0644—Valves, specific forms thereof with moving parts rotary valves
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Dispersion Chemistry (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Hematology (AREA)
- Clinical Laboratory Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Multiple-Way Valves (AREA)
Abstract
A kind of micro-fluidic chip with rotary valve structure, including valve element, substrate and egative film, fitted with egative film in one face of the substrate, another face of the substrate is provided with valve chamber and runner, at least two valve chamber through holes are provided with the valve chamber, each valve chamber through hole is connected with runner respectively, and the import being connected respectively with runner, outlet are additionally provided with the substrate；The valve element includes sealant and supporting layer, and the sealant is located in the valve chamber, and the supporting layer is located on the sealant, and the sealant is provided with ALT-CH alternate channel；During supporting layer rotation, the sealant rotation is driven, the ALT-CH alternate channel is connected with least two valve chamber through holes, it is of the invention to only need to a valve element and just realize freely changing and lead to and closing for different streams, meet the control requirement of a variety of streams；Improve production efficiency；The control of stream can just be realized by only needing to rotary spool, and control method is simply efficient.
Description
Technical field
The present invention relates to nucleic acid amplification detection technique field, more particularly to a kind of micro-fluidic core with rotary valve structure
Piece.
Background technology
Micro-fluidic chip is that based on micro electro mechanical processing technology, network is formed on chip by micro-pipe road, with controllable micro-
Stream runs through whole system and completes a kind of technology of various biological and chemical processes.Develop early stage in microfluidic chip technology,
Chip capillary cataphoresis is its mainstream technology, and chip used simple in construction, function is single；In recent years, micro-fluidic chip start to
Functionalization, integrated direction are developed rapidly, the important biology such as nucleic acid amplification reaction, immune response, cell cracking and change
Process turns into new focus.With the development of industry, the technology of micro-fluidic chip, which is applied, enters the clinical practice stage, is related to examination
Will be met on the Complicated Flows such as mixing, reaction, the pre-treatment of agent, chip structure the switching of a variety of streams, it is logical the control such as close, this
It is required for accurate reliable valve arrangement to complete.
Current detection chip has a variety of, such as CN1996009B, CN101590389A, US6627159,
US20050199500A1、US2004120856A1、US6919058B2、US20030166265A1、 WO9533986A1.But this
A little chips are without valve arrangement or using simply logical valve closing structure, it is difficult to carry out Complicated Flow.
Requirement in actual use to the valve of chip has：
1st, feature is strong.Valve realizes the various control function of stream with minimum valve as far as possible as the core cell of chip, such as many
The switching of individual stream is closed with logical.
2nd, it is reliable.Valve usually requires contact liq reagent or gaseous reagent, it is necessary to assure reagent No leakage, switching are rapid,
This needs corrosion-resistant valve, high-low temperature resistant, wear-resistant.
3rd, control simple.Chip is generally supported the use with instrument, and valve control is simpler, and Instrument Design manufacture is easier.
4th, batch micro operations.The sales volume of clinical market is generally very big and inexpensive, and this requires chip will be from price just
Suitable material, and processing and forming technology is ripe；Wet etching can not be used, because the process-cycle is long.
The content of the invention
It is an object of the invention to provide a kind of micro-fluidic chip with rotary valve structure, it can meet a variety of streams
Control is required, and sealed reliable, control method are simple, moreover it is possible to meet batch micro operations, requirement with low cost.
What the present invention was realized in：A kind of micro-fluidic chip with rotary valve structure, including valve element, substrate and bottom
Piece a, face of the substrate is fitted with egative film, and another face of the substrate is provided with valve chamber and runner, the valve chamber
Provided with least two valve chamber through holes, each valve chamber through hole is connected with runner respectively, be additionally provided with the substrate respectively with
Import, the outlet of runner connection；The valve element includes sealant and supporting layer, and the sealant is located in the valve chamber, institute
State supporting layer to be located on the sealant, the sealant is provided with ALT-CH alternate channel；During the supporting layer rotation, the sealing is driven
Layer rotation, makes the ALT-CH alternate channel be connected with least two valve chamber through holes.
Above-mentioned valve chamber includes the sunk structure being located on substrate.Above-mentioned ALT-CH alternate channel refers to be located on sealant
Passage, it is when sealant rotates, with valve chamber through hole in the state for connecting or closing so that realize stream runner it
Between free switching or logical close.
Using above-mentioned technical proposal, it is only necessary to which a valve element can just realize freely changing and lead to and closing for different streams, full
The control requirement of a variety of streams of foot；Improve production efficiency；The control of stream can just be realized by only needing to rotation supporting layer, be controlled
Method processed is simply efficient.
As a further improvement on the present invention, in addition to the gland that is located above the valve chamber, the top of the gland
Provided with gland hole, the bottom of the gland is connected with substrate, and the gland connects to form valve element accommodating chamber with the inside of valve chamber；
The supporting layer and sealant are located in the valve element accommodating chamber, the supporting layer and the tip contact of the valve element accommodating chamber,
The diameter in the gland hole is less than the diameter of the supporting layer, and the diameter of the gland is more than the diameter of supporting layer.
Using this technical scheme, supporting layer, supporting layer are fixed by gland, valve chamber, the gland is to supporting layer
Pressure, makes the sealant sealing of lower section more firm, and because the diameter of gland is more than the diameter of supporting layer, therefore supporting layer is remained to
Sealant is driven flexibly to be rotated, user can carry out rotation control, the diameter in gland hole by gland hole to supporting layer
Diameter than supporting layer is small, therefore supporting layer will not be run out of from gland.
As a further improvement on the present invention, the top of the supporting layer is provided with rotation fixing component, and the rotation is fixed
Lower section of the component in the gland hole.In the use of the present invention, can be contacted by the swingle of external instrument with supporting layer, enter
Row rotation control, need to only insert swingle in the rotation fixing component, it is possible to by external instrument to described micro-fluidic
The stream of chip is switched over or the logical control closed.
As a further improvement on the present invention, the rotation fixing component includes groove；Further, the groove includes
One word groove and cross recess.It is, because its is simple in construction, easily to produce, use using groove structure.
As a further improvement on the present invention, the sealant is elastic polymer material；The supporting layer and sealant
Total height be more than the valve element accommodating chamber depth.Using this technical scheme, the sealant is in confined state, it is ensured that
Reagent will not be volatilized or be revealed flow through the ALT-CH alternate channel of sealant when.
As a further improvement on the present invention, the elastic polymer material is one in thermoplastic elastomer (TPE) or rubber
Kind.Using thermoplastic elastomer (TPE) or rubber, first make use of its corrosion-resistant, high-low temperature resistant, wear-resistant and cheap advantage,
Second make use of its flexible, and yielding advantage is not allowed after compression.
As a further improvement on the present invention, the supporting layer is makrolon, cyclenes hydrocarbon type copolymer, thermoplastic elastic
One kind in body, copper or stainless steel.The supporting layer can be identical or different material from sealant.Using this technical side
Case, the supporting layer is wear-resistant, cheap.
As a further improvement on the present invention, the substrate be makrolon, it is polymethyl methacrylate, polypropylene, poly-
One kind in styrene, PET or cyclenes hydrocarbon type copolymer.Because sealant is in compressive state, cause whole
The chip of body is easily deformed, therefore substrate is necessary for sufficiently rigid material to ensure the long-time stability of said chip, adopts
With above-mentioned technical proposal, the rigidity of substrate is stronger, and rigid support is provided for valve element, gland.
As a further improvement on the present invention, the gland is in makrolon, cyclenes hydrocarbon type copolymer, copper or stainless steel
One kind.Using this technical scheme, the gland is wear-resistant, cheap.
As a further improvement on the present invention, the combination of the sealant and supporting layer is chemical glue sticking, laser
Weld, inlay, one kind in buckle or injection molding；The combination of the gland and substrate is hot-press sealing, high intensity
One kind in chemical glue sticking, laser welding, nesting or buckle.
As a further improvement on the present invention, the combination of the sealant and supporting layer is injection molding, the pressure
The combination of lid and substrate is to snap connection.In this way, the sealant and supporting layer are connected firmly, the pressure
The connected mode of lid and substrate flexibly, can be dismantled as needed.
The application method of this micro-fluidic chip with rotary valve structure is：The swingle of external instrument is inserted into branch
In the groove for supportting layer, swingle drives supporting layer rotation, so as to adjust the direction of the ALT-CH alternate channel on sealant, makes ALT-CH alternate channel
From different flow passages, or the corresponding runner of closing.Under current intelligence, stream enters a valve from a runner of substrate
Chamber through hole, subsequently into the ALT-CH alternate channel of sealant, and then enters another valve chamber through hole, from another runner of substrate
Outflow.In the case of closing, ALT-CH alternate channel does not connect valve chamber through hole, and reagent can not flow out.
Compared with prior art, the beneficial effects of the invention are as follows：
1st, feature is strong.The present invention need to only use a rotary valve structure to can be achieved with freely changing for multiple streams and be closed with logical,
Realize the obtainable effect of multiple logical valve closing ability in the prior art.
2nd, it is reliability and durability.Sealant, the substrate contacted in the present invention with stream is corrosion resistant high polymer material, resistance to height
It is low temperature, wear-resistant.
3rd, control simple.The present invention is only needed to manually or using the swingle of external instrument, can be achieved with operation, operation
Simply, it is to avoid complicated Instrument Design.
4th, batch micro operations.As a rule, the material used in the present invention is common material, cheap and easy to get, note
Modeling technique, to snap connection be medical device industry conventional process, easily realizes batch production.
Brief description of the drawings
Fig. 1 is a kind of explosive view of the micro-fluidic chip with rotary valve structure.
Fig. 2 is a kind of sectional view of the micro-fluidic chip with rotary valve structure.
Fig. 3 is a kind of front view of the micro-fluidic chip with rotary valve structure.
Fig. 4 is a kind of structural representation of the micro-fluidic chip with rotary valve structure.
Fig. 5 is a kind of structural representation of the micro-fluidic chip with rotary valve structure.
Fig. 6 is a kind of structural representation of the micro-fluidic chip with rotary valve structure.
Fig. 7 is a kind of structural representation of the micro-fluidic chip with rotary valve structure.
Fig. 8 is a kind of structural representation of the micro-fluidic chip with rotary valve structure.
Fig. 9 is a kind of structural representation of the micro-fluidic chip with rotary valve structure.
Brief description of the drawings：1- valve elements, 11- sealants, 110- ALT-CH alternate channels, 12- supporting layers, 120- grooves, 2- substrates, 21-
Valve chamber, 210- valve chamber through holes, 3- glands, 31- glands hole, 4- egative films, 501-A streams, 502-B streams, 503-C streams,
504-D streams, 505-E streams, 506-F streams, 507-G streams.
Embodiment
In order to illustrate more clearly of the embodiment of the present application or technical scheme of the prior art, below in conjunction with the accompanying drawings and specifically
The present invention is further described for embodiment.
Experimental method used in following embodiments is conventional method unless otherwise specified.
Material, reagent used etc., unless otherwise specified, are commercially obtained in following embodiments.
In following embodiments, chip manufacturing technology and application method are micro-fluidic chip field and field of biological detection
Routine techniques and method.
Embodiment 1
A kind of micro-fluidic chip with rotary valve structure, including valve element, substrate and egative film, a face of the substrate and egative film
Laminating, another face of the substrate, which is provided with valve chamber and runner, the valve chamber, is provided with least two valve chamber through holes, institute
State each valve chamber through hole to be connected with runner respectively, the import being connected respectively with runner, outlet are additionally provided with the substrate；The valve
Core includes sealant and supporting layer, and the sealant is located in the valve chamber, and the supporting layer is located on the sealant, institute
Sealant is stated provided with ALT-CH alternate channel；During supporting layer rotation, drive the sealant rotation, make the ALT-CH alternate channel with least
Two valve chamber through hole connections.
Above-mentioned valve chamber is the sunk structure being located on substrate.Above-mentioned ALT-CH alternate channel refers to be located at logical on sealant
Road, it is when sealant rotates, with valve chamber through hole in the state for connecting or closing, so as to realize stream between runner
Free switching or logical close.
Using above-mentioned technical proposal, it is only necessary to which a valve element can just realize freely changing and lead to and closing for different streams, full
The control requirement of a variety of streams of foot；Improve production efficiency；The control of stream can just be realized by only needing to rotation supporting layer, be controlled
Method processed is simply efficient.
Further, in addition to the gland that is located above the valve chamber, the top of the gland is provided with gland hole, described
The bottom of gland is connected with substrate, and the gland connects to form valve element accommodating chamber with the inside of valve chamber；The supporting layer and close
Sealing is located in the valve element accommodating chamber, the tip contact of the supporting layer and the valve element accommodating chamber, the gland hole it is straight
Footpath is less than the diameter of the supporting layer, and the diameter of the gland is more than the diameter of supporting layer.
Using this technical scheme, supporting layer, supporting layer are fixed by gland, valve chamber, the gland is to supporting layer
Pressure, makes the sealant sealing of lower section more firm, and because the diameter of gland is more than the diameter of supporting layer, therefore supporting layer is remained to
Sealant is driven flexibly to be rotated, user can carry out rotation control, the diameter in gland hole by gland hole to supporting layer
Diameter than supporting layer is small, therefore supporting layer will not be run out of from gland.
Further, the top of the supporting layer is provided with rotation fixing component, and the rotation fixing component is in the gland
The lower section in hole.In the use of the present invention, can be contacted by the swingle of external instrument with supporting layer, rotation control is carried out, only
Swingle need to be inserted in the rotation fixing component, it is possible to the stream of the micro-fluidic chip is carried out by external instrument
Switching or the logical control closed.
Further, the rotation fixing component includes groove；Further, the groove includes a word groove and cross
Groove.It is, because its is simple in construction, easily to produce, use using groove structure.
Embodiment 2
On the basis of embodiment 1, the sealant is elastic polymer material；The total height of the supporting layer and sealant is big
In the depth of the valve element accommodating chamber.Using this technical scheme, the sealant is in confined state, it is ensured that reagent is in stream
When the ALT-CH alternate channel of sealed layer, it will not volatilize or reveal.
Further, the elastic polymer material is one kind in thermoplastic elastomer (TPE) or rubber.Using thermoplastic elastomehc
Property body or rubber, first make use of its corrosion-resistant, high-low temperature resistant, wear-resistant and cheap advantage, and second make use of it to have
It is flexible, yielding advantage is not allowed after compression.
Further, the supporting layer is makrolon, cyclenes hydrocarbon type copolymer, thermoplastic elastomer (TPE), copper or stainless steel
In one kind.The supporting layer can be identical or different material from sealant.Using this technical scheme, the supporting layer
It is wear-resistant, it is cheap.
Further, the substrate be makrolon, it is polymethyl methacrylate, polypropylene, polystyrene, poly- to benzene two
One kind in formic acid second diester or cyclenes hydrocarbon type copolymer.
In embodiment 2, the effect of substrate has two：
1st, the structures such as valve chamber, runner, import, outlet are formed, with converging roads switching, and subsequent reactions process are carried out.
2nd, be valve element, gland rigid support is provided.Because sealant is in compressive state, overall chip is caused easily to become
Shape, therefore substrate is necessary for sufficiently rigid material to ensure the long-time stability of said chip, using above-mentioned technical proposal,
The rigidity of substrate is stronger, and rigid support is provided for valve element, gland.
Further, the gland is one kind in makrolon, cyclenes hydrocarbon type copolymer, copper or stainless steel.Using this
Technical scheme, the gland is wear-resistant, cheap.
Further, the egative film is one kind in makrolon or cyclenes hydrocarbon type copolymer.
Further, the combination of the sealant and supporting layer be chemical glue sticking, laser welding, inlay, buckle
Or one kind in injection molding；The combination of the gland and substrate is hot-press sealing, the chemical glue sticking of high intensity, laser
One kind in welding, nested or buckle.
In embodiment 2, the effect of valve element has two：
1st, effective sealing of liquid or gaseous reagent is ensured.Stream switching in sealing ensured by the sealant of valve element, when
After gland is combined with substrate, sealant is in compressive state, it is ensured that the sealing of stream；Simultaneously as sealant have it is excellent
Good decay resistance, it can be ensured that the sealing property of various property reagents under condition of different temperatures.
2nd, the stream of control liquid or gaseous reagent switches and led to and closes.Due to turning on the groove on supporting layer, sealant
Change passage, the runner of substrate has corresponding position relationship, as long as therefore control external instrument swingle angle just can control
The switching of stream processed and logical close.
The application method of this micro-fluidic chip with rotary valve structure is：The swingle of external instrument is inserted into groove
Interior, swingle drives supporting layer rotation, and then adjusts the direction of the ALT-CH alternate channel on sealant, makes ALT-CH alternate channel and different stream
Road is connected, or closes corresponding runner.Under current intelligence, stream enters a valve chamber through hole from a runner of substrate,
And then enter the ALT-CH alternate channel of sealant, another valve chamber through hole is then again introduced into, is flowed out from another runner of substrate.
In the case of closing, ALT-CH alternate channel does not connect valve chamber through hole, and reagent can not flow out.
Compared with prior art, beneficial effect is for embodiment 1, embodiment 2：
1st, feature is strong.The present invention need to only use a rotary valve structure to can be achieved with freely changing for multiple streams and be closed with logical,
Realize the obtainable effect of multiple logical valve closing ability in the prior art.
2nd, it is reliability and durability.Sealant, the substrate contacted in the present invention with stream is corrosion resistant high polymer material, resistance to height
It is low temperature, wear-resistant.
3rd, control simple.The present invention is only needed to manually or using the swingle of external instrument, can be achieved with operation, operation
Simply, it is to avoid complicated Instrument Design.
4th, batch micro operations.As a rule, the material used in the present invention is common material, cheap and easy to get, note
Modeling technique, to snap connection be medical device industry conventional process, easily realizes batch production.
Embodiment 3
On the basis of embodiment 2, the present invention will be described by the present embodiment combination Fig. 1-Fig. 5.
A kind of micro-fluidic chip with rotary valve structure as shown in Figure 1-Figure 3, including valve element 1, substrate 2, the and of gland 3
Egative film 4.The maximum gauge of substrate 2 is 5mm, and the thickness of gland 3 is 2mm, and the thickness of egative film 4 is 1mm, the substrate 2, gland 3
It is that makrolon material is made with egative film 4, the substrate 1, gland 3 and egative film 4 pass through injection molding respectively.
Egative film is combined with substrate by chemical glue bonding way.Using this technical scheme, the gland and substrate
Be connected firmly.
The substrate 2 is provided with a valve chamber 21 and runner, and valve chamber 21 is on the top of substrate 2, and runner is in substrate 2
Provided with two valve chamber through holes 210 in bottom, the valve chamber 21, each valve chamber through hole 210 is connected with runner respectively, institute
State and an import being connected respectively with runner and one outlet are additionally provided with substrate 2.The depth of valve chamber 21 is 3.5mm, institute
The width for stating runner is 0.5mm, and the depth of the runner is 0.5mm.
The valve element 1 includes sealant 11 and supporting layer 12, and the sealant 11 is located in the valve chamber 21, the branch
Support layer 12 is located on the sealant 11, and the sealant 11 is provided with ALT-CH alternate channel 110.Wherein, the valve element 1 is cylinder,
The total height of the valve element 1 is 3.6mm, a diameter of 5mm of valve element 1.The supporting layer 12 is made up of makrolon material, described
The height of supporting layer 12 is 3mm；The sealant 11 is made up of thermoplastic elastomer (TPE), and the hardness of the sealant 11 is 70 degree,
The height of sealant 11 is 0.6mm, and the supporting layer 12, sealant 11 pass through injection moulding integrated molding.
Fluted 120 on the supporting layer 12 of valve element 1, long 5mm, wide 2mm, deep 2mm, the effect of groove 120 are to coordinate outside
The swingle of instrument, is easy to instrument controlling to rotate valve angle.
Gland 3 is provided with gland hole 31, and a diameter of 3mm in gland hole 31, the diameter than supporting layer 12 is small, so can guarantee that branch
Support layer 12 is not run out of out of gland 3.
Gland is combined with substrate by laser welding mode, and the gland connects to form valve element receiving with the inside of valve chamber
Chamber.In this way, the sealant and supporting layer are connected firmly.
The height of valve element is more bigger than valve element accommodating chamber depth, and sealant decrement is 0.1mm, so when chip portfolio is finished
Afterwards, the sealant of valve element is in compressive state.There are ALT-CH alternate channel, long 3mm, wide 1mm, deep 0.6mm on the sealant of valve element.
The application method of the above-mentioned micro-fluidic chip with rotary valve structure is to comprise the following steps：
Step S1, as shown in figure 4, under original state, the ALT-CH alternate channel is not connected with the valve chamber through hole, stream is in closing
State, reagent can not pass through valve element；
Step S2, as shown in figure 5, the swingle of external instrument is inserted in groove, drives supporting layer to be rotated by 90 °, now seals
The ALT-CH alternate channel of layer rotates with 90 °, and the ALT-CH alternate channel just connects two valve chamber through holes, and stream is in opening.
Reagent enters a valve chamber through hole from a runner of substrate, and then enters ALT-CH alternate channel, subsequently into another valve chamber
Through hole, then flowed out from another runner of substrate.
Found by the test to embodiment 2, the depth of valve element accommodating chamber is crucial, and the too deep then valve element of valve element accommodating chamber is close
Feng Buyan, valve element accommodating chamber is too shallow, and valve element is difficult rotation.For the sealant being made of thermoplastic elastomer (TPE), work as sealing
The hardness of layer is 70 degree, and when the decrement that sealant is found after tested is 0.1 ~ 0.2mm, sealing property, flexibility of revolution is best.
Using the technical scheme of embodiment 3, the micro-fluidic chip with rotary valve structure it is simple in construction, material is easy
, easily by batch micro operations；The logical control closed of flow path can be just realized by a valve element, feature is strong；It is simple to operate；
Sealant and substrate are corrosion-resistant, and good airproof performance is reliability and durability.
Embodiment 4
On the basis of embodiment 2, the present embodiment combination Fig. 6-Fig. 7 is illustrated to invention.
A kind of micro-fluidic chip with rotary valve structure, the gland be thickness 1mm stainless steel plate, the gland with
The combination of substrate is to snap connection.Using this technical scheme, the connected mode of the gland and substrate is flexible, Ke Yigen
According to need dismounting.
The substrate is provided with a valve chamber and runner, the valve chamber provided with seven valve chamber through holes, described each
Valve chamber through hole is connected with runner respectively, forms A streams 501, B streams 502, C streams 503, D streams 504, E streams 505, F streams
Road 506 and G streams 507.
The sealant is provided with two ALT-CH alternate channels, relative position relation such as Fig. 6 or Fig. 7 of described two ALT-CH alternate channels
It is shown.
The application method of the above-mentioned micro-fluidic chip with rotary valve structure is to comprise the following steps：
Step H1, as shown in fig. 6, under original state, the first ALT-CH alternate channel connection A streams 501 and G streams 507 of valve element, the
Two ALT-CH alternate channel connection F streams 506 and E streams 505.
Step H2, as shown in fig. 7, the swingle of external instrument is inserted into groove, drives supporting layer to turn clockwise 90 °,
Now first ALT-CH alternate channel and second ALT-CH alternate channel not with flow passage, each stream be in closed mode.
Using the technical scheme of embodiment 4, the micro-fluidic chip with rotary valve structure it is simple in construction, material is easy
, easily by batch micro operations；The control that the free switching of flow path, the logical of stream are closed, work(can just be realized by a valve element
Energy property is strong；It is simple to operate；Sealant and substrate are corrosion-resistant, and good airproof performance is reliability and durability, and the buckle-type design of gland and substrate is held
It is quick detachable.
Embodiment 5
The present invention will be described by the present embodiment combination Fig. 8-9.
A kind of micro-fluidic chip with rotary valve structure, the difference of itself and embodiment 4 is：Two on the sealant
The relative position relation of individual ALT-CH alternate channel is different, as shown in Fig. 8 or Fig. 9.
The application method of the above-mentioned micro-fluidic chip with rotary valve structure is to comprise the following steps：
Step J1, as shown in figure 8, under original state, first ALT-CH alternate channel of valve element is not connected with runner, second conversion is logical
Road connection F streams 506 and E streams 505.
Step J2, as shown in figure 9, the swingle of external instrument is inserted into groove, 90 ° of band movable valve plug rotate counterclockwise, this
When first ALT-CH alternate channel connection A streams 501 and G streams 507, first ALT-CH alternate channel do not connect with runner.
Embodiment 4, the technical scheme of embodiment 5 are also shown that the valve element can be set according to the actual demand of user
Meter, is solved the switching of multiple streams and is led to close, function is strong, simple to operate by a valve element.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention
Any modifications, equivalent substitutions and improvements made within refreshing and principle etc., should be included in the scope of the protection.
Claims (10)
1. a kind of micro-fluidic chip with rotary valve structure, it is characterised in that including valve element, substrate and egative film, the substrate
A face fitted with egative film, another face of the substrate, which is provided with valve chamber and runner, the valve chamber, is provided with least two
Individual valve chamber through hole, each valve chamber through hole is connected with runner respectively, is additionally provided with what is be connected respectively with runner on the substrate
Import, outlet；The valve element includes sealant and supporting layer, and the sealant is located in the valve chamber, and the supporting layer is set
On the sealant, the sealant is provided with ALT-CH alternate channel；During the supporting layer rotation, the sealant rotation is driven, is made
The ALT-CH alternate channel is connected with least two valve chamber through holes.
2. the micro-fluidic chip according to claim 1 with rotary valve structure, it is characterised in that also described including being located at
Gland above valve chamber, the top of the gland is provided with gland hole, and the bottom of the gland is connected with substrate, the gland with
The inside of valve chamber connects to form valve element accommodating chamber；The supporting layer and sealant are located in the valve element accommodating chamber, the branch
Layer and the tip contact of the valve element accommodating chamber are supportted, the diameter in the gland hole is less than the diameter of the supporting layer, the gland
Diameter be more than supporting layer diameter.
3. the micro-fluidic chip according to claim 2 with rotary valve structure, it is characterised in that the top of the supporting layer
End is provided with rotation fixing component, lower section of the rotation fixing component in the gland hole.
4. the micro-fluidic chip according to claim 2 with rotary valve structure, it is characterised in that the sealant is bullet
Property high polymer material；The total height of the supporting layer and sealant is more than the depth of the valve element accommodating chamber.
5. the micro-fluidic chip according to claim 3 with rotary valve structure, it is characterised in that structure is fixed in the rotation
Part includes groove.
6. the micro-fluidic chip according to claim 4 with rotary valve structure, it is characterised in that the elastic polymer
Material is one kind in thermoplastic elastomer (TPE) or rubber.
7. the micro-fluidic chip according to claim 4 with rotary valve structure, it is characterised in that the supporting layer is poly-
One kind in carbonic ester, cyclenes hydrocarbon type copolymer, thermoplastic elastomer (TPE), copper or stainless steel.
8. the micro-fluidic chip according to claim 4 with rotary valve structure, it is characterised in that the substrate is poly- carbon
In acid esters, polymethyl methacrylate, polypropylene, polystyrene, PET or cyclenes hydrocarbon type copolymer
It is a kind of.
9. the micro-fluidic chip according to claim 4 with rotary valve structure, it is characterised in that the gland is poly- carbon
One kind in acid esters, cyclenes hydrocarbon type copolymer, copper or stainless steel.
10. the micro-fluidic chip with rotary valve structure according to claim any one of 2-9, it is characterised in that described
The combination of sealant and supporting layer is chemical glue sticking, laser welding, inlay, one kind in buckle or injection molding；
The combination of the gland and substrate is hot-press sealing, one in the chemical glue sticking of high intensity, laser welding, nested or buckle
Kind.
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| CN201710433647.7A CN107096580A (en) | 2017-06-09 | 2017-06-09 | A kind of micro-fluidic chip with rotary valve structure |
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| CN201710433647.7A CN107096580A (en) | 2017-06-09 | 2017-06-09 | A kind of micro-fluidic chip with rotary valve structure |
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ID=59660631
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| CN201710433647.7A Pending CN107096580A (en) | 2017-06-09 | 2017-06-09 | A kind of micro-fluidic chip with rotary valve structure |
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| CN109058582A (en) * | 2018-09-07 | 2018-12-21 | 深圳市刚竹医疗科技有限公司 | Valve gear |
| CN110173572A (en) * | 2019-06-17 | 2019-08-27 | 凡知医疗科技(江苏)有限公司 | A kind of microfluidic control valve mechanism |
| CN110856823A (en) * | 2018-08-22 | 2020-03-03 | 厦门大学 | Microfluidic chip and operation method thereof |
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| CN110935493A (en) * | 2019-11-28 | 2020-03-31 | 北京乐普医疗科技有限责任公司 | Micro-fluidic chip and detection method for detection by using same |
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| WO2023189118A1 (en) * | 2022-03-30 | 2023-10-05 | 富士フイルム株式会社 | Test container and nucleic acid test method |
| WO2023189119A1 (en) * | 2022-03-30 | 2023-10-05 | 富士フイルム株式会社 | Test container and nucleic acid test method |
| CN114985027B (en) * | 2022-06-13 | 2023-09-26 | 武汉纳达康生物科技有限公司 | Microfluidic valve and application method thereof |
| CN114985027A (en) * | 2022-06-13 | 2022-09-02 | 广东粤港澳大湾区国家纳米科技创新研究院 | Micro-fluidic valve and application method thereof |
| CN115155682A (en) * | 2022-06-30 | 2022-10-11 | 中国科学院苏州生物医学工程技术研究所 | Micro-fluidic chip based on rotary valve and detection method |
| CN115138409A (en) * | 2022-06-30 | 2022-10-04 | 中国科学院苏州生物医学工程技术研究所 | Rotary valve suitable for microfluidic system integration |
| CN115155682B (en) * | 2022-06-30 | 2024-03-12 | 中国科学院苏州生物医学工程技术研究所 | Microfluidic chip based on rotary valve and detection method |
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Application publication date: 20170829 |