CN107095829A - A kind of bacteriostatic hand sanitizer containing nanometer carbon crystal element and preparation method thereof - Google Patents

Abstract

The present invention discloses a kind of bacteriostatic hand sanitizer containing nanometer carbon crystal element, belongs to commodity technical field, comprises the following raw materials by weight percent：Alkylpolyoxyethylene sodium sulphate 8.0 12.0%, Cocoamidopropyl betaine 6.0 10.0%, Dodecyl Glucoside 4.0 8.0%, coconut oil diethanol amine 2.0 6.0%, modified Nano carbon crystal element 0.05 2.0%, NMF 2.0 5.0%, mixed plant extract solution 0.05 2%, sodium citrate 0.01 2.0%, citric acid 0.05 0.3%, preservative 0.1 2.0%, deionized water 65.0 85.0%.Its preparation method is provided simultaneously.Citric acid is added in the bacteriostatic hand sanitizer containing nanometer carbon crystal element of the present invention, it ensure that hand cleanser has good clean result in hard water, the pH value of hand cleanser is can adjust simultaneously, approach the pH value of hand cleanser and the acid-base value of human body skin, stimulation will not be produced to skin, be a kind of health and safety, non-stimulated bacteriostatic hand sanitizer.

Description

A kind of bacteriostatic hand sanitizer containing nanometer carbon crystal element and preparation method thereof

Technical field

The invention belongs to commodity technical field, and in particular to a kind of bacteriostatic hand sanitizer containing nanometer carbon crystal element and its system Preparation Method.

Background technology

For a long time, it has always been believed that " illness finds its way in by the mouth ", but research has shown that, numerous diseases are from " hand enters ", a lot Pathogen is taken advantage of a weak point by hand feed mouthful, or by location contacts such as hand and nose, eyes, therefore, is washed one's hands as preventing One of maximally effective approach of disease, usually using hand cleanser when washing one's hands, what most consumers were taken a fancy to when buying hand cleanser Its antibacterial or sterilizing function, most consumers think antibacterial/antibacterial hand sanitizer compared with soap, more can effectively sterilize and It is antibacterial.

At present, the common commercially available bacteriostatic hand sanitizer of China is mainly the effect for reaching that sterilization is antibacterial by adding chemical raw material Really, its antipathogenic composition mainly has o-phenyl phenol (OPP), Triclosan (DP300), chloro- 3, the 5- xylenols of 4- (PCMX), TCC (TCC) and bromogeramine thimerosal etc., above-mentioned traditional antiseptic are usually to human body and ring Border has what is necessarily endangered, and the hand cleanser containing above-mentioned antipathogenic composition is used for a long time can cause larger stimulation to skin, and skin is easy There is dry, coarse and chap phenomenon, furthermore, if cleaning not thorough during use, chemical raw material can be incoming by hand It is intraoral, larger harm is produced to human body.It is reported that the material containing phenyl ring can influence the degradation capability of microorganism, so as to make Into secondary pollution.Therefore develop a kind of clean effect it is good, with antibacterial ability, safe, non-stimulated, environmentally friendly and low cost New hand sanitizer it is significant.

The content of the invention

Present invention aims at providing, a kind of clean effect is good, safe, non-stimulated antibacterial the washing containing nanometer carbon crystal element Hand liquid, while providing the another goal of the invention that its corresponding preparation method is the present invention.

Based on above-mentioned purpose, the present invention takes following technical scheme：

A kind of bacteriostatic hand sanitizer containing nanometer carbon crystal element, is comprised the following raw materials by weight percent：Dodecyl polyoxy second Alkene ether sodium sulfate 8.0-12.0%, Cocoamidopropyl betaine 6.0-10.0%, Dodecyl Glucoside 4.0-8.0%, coconut oil two Monoethanolamine 2.0-6.0%, modified Nano carbon crystal element 0.05-2.0%, NMF 2.0-5.0%, mixed plant extract solution 0.05-2%, Sodium citrate 0.01-2.0%, citric acid 0.05-0.3%, preservative 0.1-2.0%, deionized water 65.0-85.0%.

The NMF is the mixture of glycerine and NMF；Mixed plant extract be selected from extract solution from aloe, Two or three of mixture in rose extract and SIMMONDSIA CHINENSIS SEED OIL extract solution.

The modified Nano carbon crystal element is made by following methods：Under rotating speed is 1500-2000n/min, to nanometer carbon crystal Element carries out mechanical lapping 1-2h, and after then mixing, centrifuging with the water of surfactant and 100g, centrifugation time is 30-40min, The sediment of acquirement deionized water centrifuge washing, vacuum drying is produced；Surfactant and the plain amount ratio of nanometer carbon crystal are 1:1。

The abrasive media of the mechanical lapping is diameter 4nm zirconium oxide, and the filling rate of abrasive media is 60-70%, grinding The quality of medium is 200-250g；Surfactant is Tween-80；Drying temperature is 20-30 DEG C, and drying time is 24h.

The preparation method of bacteriostatic hand sanitizer containing nanometer carbon crystal element, comprises the following steps：

1）Alkylpolyoxyethylene sodium sulphate, Cocoamidopropyl betaine and coconut oil diethanol amine are added into deionization In water, in being stirred at 50-70 DEG C to being completely dissolved, room temperature is cooled to, mixed liquor A is obtained；

2) after mixing NMF, modified Nano carbon crystal element with Dodecyl Glucoside, step 1 is added）In mixed liquor A, Ran Houyi Secondary addition mixed plant extract solution, preservative and citric acid, sodium citrate, produce bacteriostatic hand sanitizer.

Nanometer carbon crystal element is a kind of inorganic matter, environmentally friendly, safe and free of toxic and side effects, gentle to skin, non-stimulated, nano-sized carbon Oleophylic performance is good after modified for brilliant element, synergy can be played with other surfaces activating agent, so as to increase dirt-removing power；Nanometer Carbon crystal element bactericidal effect is strong, to the bactericidal effect of Escherichia coli, staphylococcus aureus and Candida albicans significantly, can be with As bacteriostatic active ingredients, it is widely used in bactericide.

Alkylpolyoxyethylene sodium sulphate has excellent wash characteristics, is that dirt-removing power is strong, excitant is low, nothing Poison, good biological degradability, resistance to hard water and cheap anion surfactant；Cocoamidopropyl betaine is compatibility Can good zwitterionic surfactant, strong detergency, excitant are small, foam is more, with excellent stability, flexibility, Antistatic behaviour, hard water resistance property；Dodecyl Glucoside is the nonionic surfactant to skin with certain protective role, is had Moisturizing power is strong, detergency is notable, non-stimulated to skin, biodegradable good advantage, and compatibility is good.

Compared with prior art, the invention has the advantages that：

The bacteriostatic hand sanitizer containing nanometer carbon crystal element that the present invention is provided has advantages below：

1）Citric acid is added in the bacteriostatic hand sanitizer containing nanometer carbon crystal element of the present invention, it is ensured that hand cleanser has in hard water Good clean result, while the pH value of adjustable hand cleanser, approaches the pH value of hand cleanser and the acid-base value of human body skin, no Stimulation can be produced to skin, be a kind of health and safety, non-stimulated bacteriostatic hand sanitizer；

2）The bacteriostatic hand sanitizer of the present invention makes to be made using anion surfactant and nonionic surfactant compounding use Hand cleanser have foam it is soft and smooth it is fine and smooth, non-stimulated, do not hurt hands, and the advantages of dirt-removing power is strong, biological degradability is good so that Reach the effect for fast and effectively cleaning both hands；

3）The bacteriostatic hand sanitizer of the present invention has the bacteriostasis of wide spectrum, has stronger sterilizing ability to microorganism, to golden yellow The sterilizing rate of color staphylococcus, Escherichia coli and Candida albicans reaches more than 99%, and nanometer carbon crystal element is mixed with preservative Play anti-corrosive antibacterial, can prevent and suppress the growth of hand cleanser bacterium, extend the storage time limit of product；

4）The bacteriostatic hand sanitizer of the present invention with the addition of NMF and mixed plant extract solution, and NMF can not only promote each component molten The stabilization of liquid, is also acted on good skin moisture-keeping；Mixed plant extract solution is by extract solution from aloe, rose extract and lotus Lotus bar oil extract liquid is mixed, and is had no toxic side effect, pollution-free, containing abundant vitamin and mineral, can be carried for skin For enough nutrition, after the hand cleanser use, there are nourishing, moisturizing to skin, can effectively prevent from using hand cleanser There is the phenomenon dried, peeled in skin afterwards；

5）The bacteriostatic hand sanitizer preparation technology of the present invention is simple, environmental protection, with good economic benefit.

Embodiment

Embodiment 1

A kind of bacteriostatic hand sanitizer containing nanometer carbon crystal element, is comprised the following raw materials by weight percent：Dodecyl polyoxy second Alkene ether sodium sulfate 8.0%, Cocoamidopropyl betaine 6.0%, Dodecyl Glucoside 8.0%, coconut oil diethanol amine 6.0%, change Property nanometer carbon crystal element 0.05%, NMF 2.0%（Glycerine 1.0%, NMF 1.0%）, mixed plant extract solution 2%（Aloe Extract solution 1.0%, the % of rose extract 0.5, the % of SIMMONDSIA CHINENSIS SEED OIL extract solution 0.5）, it is sodium citrate 2.0%, citric acid 0.05%, anti- Rotten agent 0.1%, surplus is deionized water.

The modified Nano carbon crystal element is made by following methods：Under rotating speed is 2000n/min, to nanometer carbon crystal element（Receive Rice carbon crystal element is less than or equal to 20nm nanometer carbon crystal for particle mean size）Mechanical lapping 1h is carried out, abrasive media is diameter 4nm oxygen Change zirconium, the filling rate of abrasive media is 60%, the quality of abrasive media is 250g；Then the water with Tween-80 and 100g is mixed, Under rotating speed is 10000r/min, centrifugation, 40min, the sediment deionized water centrifuge washing of acquirement, vacuum drying is produced, Drying temperature is 20 DEG C, and drying time is 24h, and Tween-80 is 1 with the plain amount ratio of nanometer carbon crystal:1.

The preparation method of bacteriostatic hand sanitizer containing nanometer carbon crystal element, comprises the following steps：

1）Alkylpolyoxyethylene sodium sulphate, Cocoamidopropyl betaine and coconut oil diethanol amine are added into deionization In water, in being stirred at 50-70 DEG C to being completely dissolved, room temperature is cooled to, mixed liquor A is obtained；

2) after mixing NMF, modified Nano carbon crystal element with Dodecyl Glucoside, step 1 is added）In mixed liquor A, Ran Houyi Secondary addition mixed plant extract solution, preservative and citric acid, sodium citrate, produce bacteriostatic hand sanitizer.

Embodiment 2

A kind of bacteriostatic hand sanitizer containing nanometer carbon crystal element, is comprised the following raw materials by weight percent：Dodecyl polyoxy second Alkene ether sodium sulfate 10.0%, Cocoamidopropyl betaine 10.0%, Dodecyl Glucoside 4.0%, coconut oil diethanol amine 2.0%, Modified Nano carbon crystal element 2.0%, NMF 5.0%（Glycerine 2.0%, NMF 3.0%）, mixed plant extract solution 0.05% （Extract solution from aloe 0.02%, rose extract 0.03%）, sodium citrate 0.01-2.0%, citric acid 0.05-0.3%, preservative 0.1-2.0%, surplus is deionized water.

The modified Nano carbon crystal element is made by following methods：Under rotating speed is 1500n/min, nanometer carbon crystal element is carried out Mechanical lapping 2h, abrasive media is diameter 4nm zirconium oxide, and the filling rate of abrasive media is 70%, and the quality of abrasive media is 200g；Then the water with Tween-80 and 100g is mixed, and under rotating speed is 10000r/min, centrifuges 30min, the sediment of acquirement Deionized water centrifuge washing is used, vacuum drying is produced, drying temperature is 30 DEG C, and drying time is 24h, Tween-80 and nano-sized carbon The amount ratio of brilliant element is 1:1.

Its preparation method is with reference to embodiment 1.

Embodiment 3

A kind of bacteriostatic hand sanitizer containing nanometer carbon crystal element, is comprised the following raw materials by weight percent：Dodecyl polyoxy second Alkene ether sodium sulfate 12.0%, Cocoamidopropyl betaine 7.0%, Dodecyl Glucoside 5.0%, coconut oil diethanol amine 4.0%, change Property nanometer carbon crystal element 0.1%, NMF 3.0%（Glycerine 1.0%, NMF 2.0%）, mixed plant extract solution 1.0%（Reed Luxuriant growth extract solution 0.1%, SIMMONDSIA CHINENSIS SEED OIL extract solution 0.9%）, sodium citrate 1.0%, citric acid 0.1%, preservative 1.0%, surplus is goes Ionized water.

The method of modifying of modified Nano carbon crystal element and the preparation method of bacteriostatic hand sanitizer are with reference to embodiment 1.

Embodiment 4

A kind of bacteriostatic hand sanitizer containing nanometer carbon crystal element, is comprised the following raw materials by weight percent：Dodecyl polyoxy second Alkene ether sodium sulfate 9.0%, Cocoamidopropyl betaine 8.0%, Dodecyl Glucoside 6.0%, coconut oil diethanol amine 3.0%, change Property nanometer carbon crystal element 1.0%, NMF 4.0%（Glycerine 2.0%, NMF 2.0%）, mixed plant extract solution 1.5%（Reed Luxuriant growth extract solution 0.4%, rose extract 0.4%, SIMMONDSIA CHINENSIS SEED OIL extract solution 0.7%）, it is sodium citrate 0.5%, citric acid 0.2%, anti- Rotten agent 0.5%, surplus is deionized water.

The method of modifying of modified Nano carbon crystal element and the preparation method of bacteriostatic hand sanitizer are with reference to embodiment 2.

The performance test of embodiment 5

PH value, gross activity thing content and the biological degradability containing the plain bacteriostatic hand sanitizer of nanometer carbon crystal of 5.1 present invention determines examination Test

In at 25 DEG C, Example 1-4 bacteriostatic hand sanitizer sample tests its pH value respectively, method of testing with reference to GB/T6368, Gross activity thing content assaying method is with reference to 8.1 in GB/T13173.2-2000, and surfactant biodegradation is with reference to GB/ T15815.Each sample does three parallel tests, and measurement result takes its average value, determination data statistics such as table 1 below.

As shown in Table 1, the pH value of bacteriostatic hand sanitizer of the invention is 6.0-6.5, non-stimulated to skin, and several surfactants are answered With using gross activity thing content in hand cleanser is improved, so as to enhance the detergency of hand cleanser, hand cleanser biology of the present invention drops Xie Du is more than 90%, and therefore, bacteriostatic hand sanitizer dirt-removing power of the invention is strong, biological degradability is good.

5.2 chmice acute Oral toxicities are tested

Selection body weight be 18-22g Kun Ming mices 20, female, male half and half, using disposable maximum test method(s), animal contaminated Dosage is 5000mg/kg (body weight), with embodiment 1, the oral gavage contamination of bacteriostatic hand sanitizer sample, gavage amount is 0.2mL/ 10g (body weight), continuous observation 14d after administration, the record various poisoning manifestations of mouse and death condition, calculate Oral toxicity LD_soValue. As a result show, animal subject observed after contamination during without obvious poisoning sign, no animal dead, to chmice acute Oral toxicity LD_soValue ＞ 5000mg/kg (body weight), illustrate hand cleanser of the bacteriostatic hand sanitizer of the present invention for actual nontoxic level, safety.

5. 3 Skin Irritation Tests

Using a Skin Irritation Test method, the healthy adult white rabbit 4 that selection body weight is 2.2-2.5kg, 24h before experiment Family's rabbit back backbone diamond wool is cut, the unhairing scope per side is respectively 3 cm × 3cm.Take the hand cleanser of the embodiment of the present invention 2 Stoste 0.5mL is applied on the skin of unhairing of rabbit back side, is washed away after 4h with warm water, and the opposite side skin of hand cleanser is not smeared Compare.Smear once daily, continuously smear 14d.24h observations dermoreaction, evaluates stimulus intensity after each smear.As a result Show, using bacteriostatic hand sanitizer stoste of the present invention as tested material, after contact 4 tested local skins of rabbit be showed no it is red and swollen, Inflammation, phenomenon, test group and the control group indifference such as fester, therefore, bacteriostatic hand sanitizer of the present invention are stimulated without any skin, can Safe handling.

5. 4 antibacterial contrast tests

Bacteriostasis property test is with reference to GB15979-2002.Test index bacterium is that Escherichia coli, staphylococcus aureus and white are read Pearl bacterium, provides by Chinese Academy of Sciences's Culture Collection.By each test organisms by Zengjing Granule and line separation training Support, the single colonies typical inoculation ordinary nutrient agar medium slant of selection, Candida albicans husky fort agar medium inclined-plane, 24h is cultivated at 37 DEG C.Fresh slant culture is washed down and diluted with the phosphate buffer (PBS) of the 10g/L containing peptone, is matched somebody with somebody Experiment bacteria suspension is made, it is standby.

Bacteriostasis property determines and is set to three groups, respectively A groups（Test group）, B groups（Control group 1）, C groups（Control group 2）, A groups Sample is the bacteriostatic hand sanitizer sample of the embodiment of the present invention 1, and B groups sample is the hand cleanser without nanometer carbon crystal element, other numbers According to 1 group of be the same as Example, the sample of C groups is commercially available bacteriostatic hand sanitizer.Using suspension quantitative test method, carried out in 20 DEG C of water-baths real Test.Hand cleanser stoste used in every group is diluted with the deionized water of same volume respectively, dilution is every group of experiment Sample liquid, adds sample liquid 5.0mL and PBS 5.0mL in sterile test tube, and every group of formula 4 is managed, be added dropwise respectively in every pipe 0.1mL bacteria suspension, is mixed immediately.Different time is acted on, takes the mL of bacterium medicine mixed liquor 0.5 to be added to containing 4.5 mL PBS bufferings Through fully mixing in liquid test tube, 0.5 mL inoculated and cultureds are taken, make viable bacteria calculating, bacteriostasis rate is calculated.Experiment is repeated 3 times, experiment knot Fruit is as shown under table 2.

As shown in Table 2, under the conditions of 20 DEG C, contrasted with commercially available anti-bacterial hand lotion, bacteriostatic hand sanitizer of the invention is imitated in antibacterial There is very big advantage on fruit, 100.00% is reached to the sterilizing rate of Escherichia coli and staphylococcus aureus；Candida albicans The equal ＞ 99.00% of sterilizing rate, with the extension of hand cleanser action time, bacteriostasis rate increase is not obvious.And B groups are tested, do not add The plain hand cleanser as bacteriostatic active ingredients of nanometer carbon crystal, without fungistatic effect.Therefore, bacteriostatic hand sanitizer of the invention is to micro- Biology has significant inhibitory action, with stronger bactericidal ability.The present invention is used as bactericidal using nanometer carbon crystal element Active component, the bacteriostatic hand sanitizer of preparation can have gentle, non-stimulated, shield concurrently while product decontamination, bactericidal is ensured The features such as skin, substantially, clean effect is good, small to skin irritatin, and has for the plain bacteriostatic hand sanitizer fungistatic effect of nanometer carbon crystal of preparation There is broad-spectrum antibacterial effect, have no toxic side effect, be a kind of hand cleanser with sterilization, decontamination and skin care multiple action.

5. 5 stability tests

The bacteriostatic hand sanitizer of the embodiment of the present invention 2 is taken to pack sample, 3 points of a formula is placed in storage 90d in 37 DEG C of insulating boxs. The sample before storage and after storage is taken, Candida albicans is pressed down by the bacteriostatic test method in 5. 4 antibacterial contrast tests Bacterium is tested, and calculates bacteriostasis rate, and result of the test is shown in Table 3.Changed by bacteriostasis rate before and after storage, judge its stability.

As shown in Table 3, the average bacteriostasis rate ＞ 98.00% of product after storage, compared with before storage, does not change, illustrates this hair substantially Bright bacteriostatic hand sanitizer stability is good.

Claims (5)

1. a kind of bacteriostatic hand sanitizer containing nanometer carbon crystal element, it is characterised in that comprise the following raw materials by weight percent：Ten Dialkyl group polyethenoxy ether sodium sulfate 8.0-12.0%, Cocoamidopropyl betaine 6.0-10.0%, Dodecyl Glucoside 4.0- 8.0%th, coconut oil diethanol amine 2.0-6.0%, modified Nano carbon crystal element 0.05-2.0%, NMF 2.0-5.0%, mixed plant are carried Take liquid 0.05-2%, sodium citrate 0.01-2.0%, citric acid 0.05-0.3%, preservative 0.1-2.0%, deionized water 65.0- 85.0%。

2. the bacteriostatic hand sanitizer as claimed in claim 1 containing nanometer carbon crystal element, it is characterised in that the NMF is glycerine With the mixture of NMF；Mixed plant extract is carried selected from extract solution from aloe, rose extract and SIMMONDSIA CHINENSIS SEED OIL Take two or three of mixture in liquid.

3. the bacteriostatic hand sanitizer as claimed in claim 1 containing nanometer carbon crystal element, it is characterised in that the modified Nano carbon crystal Element is made by following methods：Under rotating speed is 1500-2000n/min, mechanical lapping 1-2h, Ran Houyu are carried out to nanometer carbon crystal element Surfactant and 100g water mixing, centrifugation after, centrifugation time is 30-40min, and the sediment of acquirement is centrifuged with deionized water Washing, vacuum drying is produced；The amount ratio of surfactant and nanometer carbon crystal element is 1:1.

4. the bacteriostatic hand sanitizer containing nanometer carbon crystal element as claimed in claim 3, it is characterised in that the mechanical lapping is ground Grinding media is diameter 4nm zirconium oxide, and the filling rate of abrasive media is 60-70%, and the quality of abrasive media is 200-250g；Table Face activating agent is Tween-80；Drying temperature is 20-30 DEG C, and drying time is 24h.

5. the preparation method of the bacteriostatic hand sanitizer containing nanometer carbon crystal element described in claim 1, it is characterised in that including following Step：

1）Alkylpolyoxyethylene sodium sulphate, Cocoamidopropyl betaine and coconut oil diethanol amine are added into deionization In water, in being stirred at 50-70 DEG C to being completely dissolved, room temperature is cooled to, mixed liquor A is obtained；

2) after mixing NMF, modified Nano carbon crystal element with Dodecyl Glucoside, step 1 is added）In mixed liquor A, Ran Houyi Secondary addition mixed plant extract solution, preservative and citric acid, sodium citrate, produce bacteriostatic hand sanitizer.