CN107091835A - A kind of discrimination method of Idesia polycarpa seedling male and female plant - Google Patents
A kind of discrimination method of Idesia polycarpa seedling male and female plant Download PDFInfo
- Publication number
- CN107091835A CN107091835A CN201710128456.XA CN201710128456A CN107091835A CN 107091835 A CN107091835 A CN 107091835A CN 201710128456 A CN201710128456 A CN 201710128456A CN 107091835 A CN107091835 A CN 107091835A
- Authority
- CN
- China
- Prior art keywords
- female plant
- idesia polycarpa
- male
- mixed liquor
- discrimination method
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
Abstract
The invention discloses a kind of discrimination method of Idesia polycarpa seedling male and female plant, the discrimination method complex operation of Idesia polycarpa seedling male and female plant in the prior art is solved, specialized equipment need to be used, be unsuitable for the problem of basic unit's popularization and application.This method comprises the following steps：The seedling leaves 10g of the Idesia polycarpa of clean dried is taken, is shredded, phosphate buffer is added, homogenate obtains homogenate；By homogenate in being stored at room temperature after 1-2 hours, centrifuge, take supernatant 2ml, add 2 μ g/ml methyl red ethanol solution, mix, obtain mixed liquor；Mixed liquor is placed at a temperature of 30 DEG C, its color change is observed, and color change is recorded every 2 3min.Result of the present invention is accurate, and easy to operate, detection time is short, with low cost, suitable in basic unit's popularization and application.
Description
Technical field
The invention belongs to biological technical field, and in particular to a kind of discrimination method of Idesia polycarpa seedling male and female plant.
Background technology
Idesia polycarpa (Idesia polycarpa Maxim.var.vestita Diels) is Flacourtiaceae Mao Yeshan
The deciduous tree of seeds of a tung oil tree category, with high economic value and ornamental value.Idesia polycarpa is dioecian plant, and female is planted
Strain can also grow some fruits under conditions of male plants are departed from, but go for fruit and seed must be while cultivate female plant
And staminiferous plant.Idesia polycarpa fertility under natural endowment is extremely weak, therefore in plantation early stage, is entered according to the male and female of seedling plants
Professional etiquette is drawn plantation and had great importance.
The discriminating to Idesia polycarpa plant can use isozymogram or karyotype identification method in the prior art, accurately
Rate is higher.But when being identified early stage seedling, selectable enzyme class is less, the enzyme band of colour developing is few, can not distinguish sometimes
Open male and female plant.Identified using karyotype, complex operation is, it is necessary to which special instrument, is unsuitable for the popularization and application of basic unit.
Therefore a kind of discrimination method of Idesia polycarpa seedling male and female plant is provided, it is simple to operate, it is not necessary to special instrument,
Suitable for the popularization and application in basic unit, become those skilled in the art's urgent problem to be solved.
The content of the invention
The technical problem to be solved in the present invention is：A kind of discrimination method of Idesia polycarpa seedling male and female plant is provided, solved
The discrimination method complex operation of Idesia polycarpa seedling male and female plant, need to use specialized equipment, be unsuitable for pushing away in basic unit in the prior art
The problem of extensively applying.
To achieve the above object, the technical solution adopted by the present invention is as follows：
A kind of discrimination method of Idesia polycarpa seedling male and female plant disclosed by the invention, this method comprises the following steps：
A：The seedling leaves 10g of the Idesia polycarpa of clean dried is taken, is shredded, phosphate buffer is added, homogenate must be homogenized
Liquid；
B：By homogenate in being stored at room temperature after 1-2 hours, centrifuge, take supernatant, it is standby；
C：Supernatant 2ml is taken, 2 μ g/ml methyl red ethanol solution is added, mixes, obtains mixed liquor；
D：Mixed liquor obtained by step C is placed at a temperature of 30 DEG C, its color change is observed, and color is recorded every 2-3min
Change.
Preferably, the pH value of the phosphate buffer is 6.
Preferably, centrifugation centrifuges 5min for 5000rpm in the step B.
Preferably, male and female plant is judged according to the color change of the mixed liquor, specific method is：Gained in the step B
Mixed liquor is yellow, as time went on, and female plant mixed liquor goes to red by yellow in 10-15min, and staminiferous plant mixed liquor is still
Yellow.
Compared with prior art, the invention has the advantages that：
(1) Idesia polycarpa seedling male and female plant is differentiated using the inventive method, as a result accurately, easy to operate, inspection
The survey time is short, with low cost, suitable in basic unit's popularization and application.
(2) result of Idesia polycarpa seedling male and female plant is differentiated using the inventive method compared with using form differential method,
As a result it is consistent, show that this method result is accurate.
(3) the inventive method is differentiated using development process, easy to operate, it is not necessary to special instrument, with low cost, common skill
Art workman can just grasp, suitable for popularization and application, with stronger industrialization meaning.
Embodiment
With reference to specific embodiment, the invention will be further described, but do not limit the scope of the invention and apply
Scope.
Embodiment 1
The determination of phosphate buffer pH value
The seedling leaves 10g of the Idesia polycarpa of clean dried is taken, is shredded, the phosphorus of different pH value as shown in table 1 is added
Acid buffer, homogenate, obtains homogenate；By homogenate in being stored at room temperature after 1-2 hours, 5000rpm centrifugation 5min take supernatant,
It is standby；Supernatant 2ml is taken, 2 μ g/ml methyl red ethanol solution is added, mixes, obtains mixed liquor；Gained mixed liquor is placed in 30
At a temperature of DEG C, its color change is observed, and color change is recorded every 2-3min, 1 is the results are shown in Table.
The phosphate buffer result of the test table of the different pH value of table 1
| Sequence number | PH value | Result of the test |
| 1 | 5.6 | Male and female plant leaching liquor color is had any different, but longer the time required to discoloration |
| 2 | 5.8 | Male and female plant leaching liquor color is had any different, but longer the time required to discoloration |
| 3 | 6.0 | Male and female plant leaching liquor color is had any different, shorter the time required to discoloration |
| 4 | 6.2 | Male and female plant leaching liquor color indistinction |
| 5 | 6.4 | Male and female plant leaching liquor color indistinction |
As shown in Table 1, it is convenient when phosphate buffer pH value is 6.0.
Embodiment 2
Male and female plant discriminating is carried out to different Idesia polycarpa seedling using the inventive method, by acquired results with using shape
The identification result of state method compares, and see the table below.
The distinct methods identification result table of table 2
As shown in Table 2, Idesia polycarpa seedling male and female plant is differentiated using the inventive method, as a result accurately.
Above-described embodiment is only one of the preferred embodiment of the present invention, should not be taken to limit the protection model of the present invention
Enclose, as long as the present invention body design thought and mentally make have no the change of essential meaning or polishing, it is solved
Technical problem it is still consistent with the present invention, should be included in protection scope of the present invention within.
Claims (4)
1. a kind of discrimination method of Idesia polycarpa seedling male and female plant, it is characterised in that：Comprise the following steps：
A：The seedling leaves 10g of the Idesia polycarpa of clean dried is taken, is shredded, phosphate buffer is added, homogenate obtains homogenate；
B：By homogenate in being stored at room temperature after 1-2 hours, centrifuge, take supernatant, it is standby；
C：Supernatant 2ml is taken, 2 μ g/ml methyl red ethanol solution is added, mixes, obtains mixed liquor；
D：Mixed liquor obtained by step C is placed at a temperature of 30 DEG C, its color change is observed, and become every 2-3min record colors
Change.
2. a kind of discrimination method of Idesia polycarpa seedling male and female plant according to claim 1, it is characterised in that：The phosphorus
The pH value of acid buffer is 6.
3. a kind of discrimination method of Idesia polycarpa seedling male and female plant according to claim 1 or 2, it is characterised in that：Institute
State centrifugation in step B and centrifuge 5min for 5000rpm.
4. a kind of discrimination method of Idesia polycarpa seedling male and female plant according to claim 1-3 any one, its feature
It is：Male and female plant is judged according to the color change of the mixed liquor, specific method is：Gained mixed liquor is Huang in the step B
Color, as time went on, female plant mixed liquor goes to red by yellow in 10-15min, and staminiferous plant mixed liquor is still yellow.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710128456.XA CN107091835A (en) | 2017-03-06 | 2017-03-06 | A kind of discrimination method of Idesia polycarpa seedling male and female plant |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710128456.XA CN107091835A (en) | 2017-03-06 | 2017-03-06 | A kind of discrimination method of Idesia polycarpa seedling male and female plant |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107091835A true CN107091835A (en) | 2017-08-25 |
Family
ID=59648853
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710128456.XA Pending CN107091835A (en) | 2017-03-06 | 2017-03-06 | A kind of discrimination method of Idesia polycarpa seedling male and female plant |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107091835A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109724977A (en) * | 2018-12-21 | 2019-05-07 | 塔里木大学 | A kind of trees Sex judging method of simplicity |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103149159A (en) * | 2013-02-06 | 2013-06-12 | 辽宁大学 | Identification method for ginkgo seedling male and female plants |
| CN103926244A (en) * | 2014-04-22 | 2014-07-16 | 辽宁大学 | Method for identifying male and female plants of populus tomentosa seedlings |
| US20160273038A1 (en) * | 2015-03-19 | 2016-09-22 | King Saud University | Method of identifying date palm gender using scar primers |
-
2017
- 2017-03-06 CN CN201710128456.XA patent/CN107091835A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103149159A (en) * | 2013-02-06 | 2013-06-12 | 辽宁大学 | Identification method for ginkgo seedling male and female plants |
| CN103926244A (en) * | 2014-04-22 | 2014-07-16 | 辽宁大学 | Method for identifying male and female plants of populus tomentosa seedlings |
| US20160273038A1 (en) * | 2015-03-19 | 2016-09-22 | King Saud University | Method of identifying date palm gender using scar primers |
Non-Patent Citations (2)
| Title |
|---|
| 冯莉等: "芦笋雌雄株鉴定方法初探", 《浙江农业学报》 * |
| 彭子模，原惠: "用甲基红鉴定几种植物雌雄株", 《生物学通报》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109724977A (en) * | 2018-12-21 | 2019-05-07 | 塔里木大学 | A kind of trees Sex judging method of simplicity |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104711361A (en) | Method for quickly identifying purity of new watermelon species namely red peace hybrid seeds as well as primer and kit adopted by method | |
| CN103361425A (en) | Primer and method for identifying purity of cucumber 'Yuexiu No.3' hybrid seed | |
| CN109832188B (en) | Preparation method of corn double haploid | |
| CN103993073B (en) | The rapid identification method of green No. 1 cross-fertilize seed of broccoli kind platform | |
| CN107091835A (en) | A kind of discrimination method of Idesia polycarpa seedling male and female plant | |
| CN103149159A (en) | Identification method for ginkgo seedling male and female plants | |
| CN103926244A (en) | Method for identifying male and female plants of populus tomentosa seedlings | |
| CN103993070B (en) | A kind of SSR primer for exquisite joint melon hybrid seed purity qualification and method | |
| Baghyalakshmi et al. | Development and characterization of tetraploid castor plants | |
| CN105907848A (en) | SSR primer group and method for identification of purity of flue-cured tobacco variety Yunyan 87 seeds | |
| CN108707692B (en) | Method and primer for rapidly identifying purity of hybrid seed of watermelon variety' Hubei watermelon No. 16 | |
| CN113637791B (en) | Molecular marker for simultaneously identifying restorability and authenticity of pepper male sterile three-line hybrid and identification method thereof | |
| CN103627799B (en) | A kind of longan filial generation Purity method based on EST-SSR mark | |
| CN108977573A (en) | A method of seven-star radish purity of hybrid is identified using SSR molecular marker | |
| CN105613256A (en) | Watermelon hybridization breeding and hybrid identification method | |
| CN108165652A (en) | For the specific molecular marker TGMI001 of Chinese torreya seedling stage sex identification | |
| CN112725521B (en) | Dendrobium chrysotoxum SSR molecular marker primer composition and application thereof | |
| CN103131755A (en) | Method for rapidly detecting brassica oleracea var. italic hybrid purity by using SRAP mark | |
| CN108531636A (en) | A kind of molecular marked compound TJcM01 and its application for identifying muskmelon unisexual flower | |
| CN108165655A (en) | A kind of SSR marker SP_SSR19 with spinach male close linkage and its application in spinach sex identification | |
| CN108411029B (en) | The long green No. 2 hybrid seed purities identification specific marker of balsam pear and method | |
| CN107130034A (en) | A kind of method that utilization SSR marker identifies flat Europe hybrid hazel kind | |
| CN103589799B (en) | A kind ofly detect the method whether having selfed seed in cotton crossbreed prepared by seeding farmer to be measured | |
| Partelli et al. | Nutritional diagnosis of the organic Conilon coffee trees (Coffea canephora Pierre ex Froehn): suffiency range approach for leaves and soil | |
| CN107164539B (en) | SSR molecular marker for identifying purity of pepper variety XingShu Lvyan hybrid and method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20170825 |