CN107090415A - The Stenotrophomonas of one plant of pedo relict dichloro quinolinic acid that can degrade - Google Patents
The Stenotrophomonas of one plant of pedo relict dichloro quinolinic acid that can degrade Download PDFInfo
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- CN107090415A CN107090415A CN201710267838.0A CN201710267838A CN107090415A CN 107090415 A CN107090415 A CN 107090415A CN 201710267838 A CN201710267838 A CN 201710267838A CN 107090415 A CN107090415 A CN 107090415A
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- quinolinic acid
- dichloro quinolinic
- stenotrophomonas
- plant
- degrade
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
Abstract
The invention discloses one plant of can degrade dichloro quinolinic acid degradation bacteria and its application.One plant of dichloro quinolinic acid degradation bacteria provided by the present invention is Stenotrophomonas J3（Stenotrophomonassp.J3）, China typical culture collection center (CCTCC) is preserved in, preservation date is on March 16th, 2017, and deposit number is CCTCC NO:M 2017128, Stenotrophomonas is accredited as using 16S rDNA.The bacterium is Gram-negative bacteria, and bacterium colony is in yellow, circle, protuberance, opaque, neat in edge.The bacterium has the function of degraded dichloro quinolinic acid.
Description
Technical field
The invention belongs to environmental pollution microbiological treatment technical field, and in particular to one plant of pedo relict dichloro that can degrade
The Stenotrophomonas of quinolinic acid.
Background technology
Tobacco（Nicotiana tabacum）Belong to that Solanaceae is annual or limited herbaceos perennial, originating from South America,
Throughout world various regions are planted at present.Tobacco is a kind of special industrial crops, China from nineteen eighty-two carry out tobacco monopoly system with
Come, tobacco business obtains tremendous development and a mainstay industry as national economy.Tobacco is in each provinces and regions in China north and south
There is plantation, its main application is processing leaf tobacco production cigarette.Therefore, the tobacco leaf of high-quality is the important goal of tobacco leaf production, such as
The tobacco leaf of what guarantee high-quality is also the major issue that tobacco grower is concerned about.In Fujian, tobacco planting mainly based on rice cigarette crop rotation method,
But the use of the conventional agricultural chemicals such as herbicide of first crop crop paddy rice, have a strong impact on the growth of rear stubble tobacco.Tobacco is to weeding
Agent is more sensitive, with herbicide widely using in rice terrace, and tobacco leaf production is happened occasionally by the phenomenon of herbicide damage,
Great harm is brought to tobacco leaf production.
After being applied there are some researches show dichloro quinolinic acid, there is researcher to detect water near rice terrace in the U.S. Arkansas State
The residual quantity of domain dichloro quinolinic acid is up to g/l grades of μ;The environmental behaviour of dichloro quinolinic acid is applied using lisimeter simulation water rice field,
As a result show that 95% remains in 30 cm topsoils of rice terrace.The dichloro quinolinic acid remained in soil can influence tobacco to plant
The teratogenesis of strain, causes young leaves to be crispaturaed to blade back, is then developing progressively wire rat-tail shape leaf.Due to dichloro quinolinic acid
Poisoning, the yield and the output value of flue-cured tobacco are substantially reduced, and more than 80%, or even total crop failure can be reduced during serious harm.
Microbial method deteriorating pesticide residue have it is environment-friendly, it is pollution-free, the features such as efficient, therefore utilize and biological carry out ring
Border reparation is study hotspot in recent years.The present invention is used for a long time separation screening in the soil of dichloro quinolinic acid by field and obtained
Degradation bacteria strains, bacterial strain of the present invention is general Pseudomonas bacterial strain, and having not yet to see general Pseudomonas is used for pedo relict dichloroquinoline
The report of acid degradation；Bacterium source of the present invention in soil, with it is environment-friendly the characteristics of.Vega residual is released using bioanalysis to remove
Careless agent dichloro quinolinic acid can not only reduce the economic loss that poisoning brings tobacco, and to environmental protection, purification soil has not
The meaning that can be despised.
The content of the invention
Being remained for the above-mentioned dichloro quinolinic acid of solution in soil causes the problem of tobacco production and the output value are reduced, and the present invention is carried
For the Stenotrophomonas of one plant of pedo relict dichloro quinolinic acid that can degrade.The bacterial strain can effectively degrade the dichloro remained in soil
Quinolinic acid, so as to improve tobacco production.
To achieve the above object, the present invention is adopted the following technical scheme that：
The Stenotrophomonas of one plant of pedo relict dichloro quinolinic acid that can degrade, identified, the bacterial strain is Stenotrophomonas J3
（Stenotrophomonassp. J3）, it has been preserved in China typical culture collection center CCTCC, address：Wuhan, China
Wuhan University, postcode：430072；Preservation date is on March 16th, 2017, and deposit number is CCTCC NO: M
2017128。
Bacterial strain J3 of the present invention is enriched with by the following method to be obtained：
This experiment gathers soil from Fujian vega, is screened and obtained using concentration method, its screening process is as follows：
（1）The enrichment of dichloro quinolinic acid degradation bacteria：Take 0.1g soil to be mixed with 100 ml MM culture mediums, and add dichloroquinoline
Acid makes its final concentration of 100 μ g/ml, and in 30 DEG C, 150 r/min are cultivated 1 week；The ml of nutrient solution 1 is taken to add 100 ml containing same
In the fresh culture of concentration herbicidal agent, while ruling to confirm to have bacterial growth, similarity condition culture 1 in solid medium
Week；It is continuous to turn to be commissioned to train after foster 10 times, separated through plate streaking, obtain single bacterium colony bacterial strain.
（2）The liquid chromatogram measuring bacterium degradation rate：Obtained strains are respectively placed in containing 5 μ g/ml dichloro quinolinic acids and not
Cultivated in MM culture mediums containing dichloro quinolinic acid, to be not added with MM culture medium of the bacterium solution containing only same concentrations dichloro quinolinic acid as ginseng
According to being measured using high performance liquid chromatography；Sample time is respectively the 6th d and the 23rd d, and sample volume is 1 ml；By sample
Product centrifuge 10 min under the conditions of 8000 r/min, and supernatant is used for HPLC after 0.22 μm of membrane filtration and analyzed, each place
Manage three repetitions.HPLC testing conditions are：Stationary phase is C18 posts（4.6 × 250 nm, 5 μm, Agilent）；Mobile phase is methanol
+ 0.5% acetic acid water（Volume ratio is 65:35）；Flow velocity is 1.0 ml/min；Column temperature is 30 DEG C；Detection wavelength is 240 nm；Sample introduction
Volume is 10 μ l.
Utilize（2）Described in testing conditions the dichloro quinolinic acid degradation capability of the bacterial strain is detected, and by following
Formula tries to achieve its dichloro quinolinic acid degradation rate：
Bacterial strain J3 of the present invention can be used for degraded dichloro quinolinic acid.
The advantage of the invention is that：
The present invention is used for a long time separation screening in the soil of dichloro quinolinic acid by field and obtains degradation bacteria strains, is dropped using bioanalysis
Solution vega residual herbicide dichloro quinolinic acid can not only reduce the economic loss that poisoning brings tobacco, and to environmental protection,
Purification soil has the meaning that can not be despised.
Brief description of the drawings
Fig. 1 concentration methods screen the microbial strains flow chart of degradable dichloro quinolinic acid；
Fig. 2 J3 colonial morphology figures：Bacterium colony is in yellow, circle, protuberance, opaque, neat in edge；
Fig. 3 standard items dichloro quinolinic acids（5 mg/L）Chromatogram in MM culture mediums and methanol：A is MM culture mediums, and B is to add
Plus the MM culture mediums of bacterium solution, C is the MM culture mediums of addition dichloro quinolinic acid, dichloro quinolinic acids of the D for dissolving in methyl alcohol；
Fig. 4 high performance liquid chromatographies detect the standard curve of dichloro quinolinic acid；
The degradation rate to dichloro quinolinic acid after bacterial strain J3 cultures 6d and 23d is added in Fig. 5 MM culture mediums.
Embodiment
The bacterial strain J3 of embodiment 1 screening
This experiment gathers soil from vega, and the microbial strains of degradable dichloro quinolinic acid, process such as Fig. 1 are screened using concentration method
It is shown.This experiment sieving obtains the bacterial strain of one plant of soil dichloro quinolinic acid that can degrade, and is named as J3；Its screening process is such as
Under：
（1）The enrichment of dichloro quinolinic acid degradation bacteria：Take 0.1g soil to be mixed with 100 ml MM culture mediums, and add dichloroquinoline
Acid makes its final concentration of 100 μ g/ml, and in 30 DEG C, 150 r/min are cultivated 1 week；The ml of nutrient solution 1 is taken to add 100 ml containing same
In the fresh culture of concentration herbicidal agent, while ruling to confirm to have bacterial growth, similarity condition culture 1 in solid medium
Week；It is continuous to turn to be commissioned to train after foster 10 times, separated through plate streaking, obtain single bacterium colony bacterial strain.The bacterium is Gram-negative bacteria, and bacterium colony is in
Yellow, circle, protuberance, opaque, neat in edge；Its colonial morphology figure is shown in Fig. 2.
（2）The liquid chromatogram measuring bacterium degradation rate：Obtained strains are respectively placed in containing 5 μ g/ml dichloro quinolinic acids and not
Cultivated in MM culture mediums containing dichloro quinolinic acid, to be not added with MM culture medium of the bacterium solution containing only same concentrations dichloro quinolinic acid as ginseng
According to being measured using high performance liquid chromatography；Sample time is respectively the 6th d and the 23rd d, and sample volume is 1 ml；By sample
Product centrifuge 10 min under the conditions of 8000 r/min, and supernatant is used for HPLC after 0.22 μm of membrane filtration and analyzed, each place
Manage three repetitions.HPLC testing conditions are：Stationary phase is C18 posts（4.6 × 250 nm, 5 μm, Agilent）；Mobile phase is methanol
+ 0.5% acetic acid water（Volume ratio is 65:35）；Flow velocity is 1.0 ml/min；Column temperature is 30 DEG C；Detection wavelength is 240 nm；Sample introduction
Volume is 10 μ l.Its chromatogram is shown in Fig. 3, as a result show under this condition, can be very good by the target peak of dichloro quinolinic acid
Separated with impurity.
（3）Make standard curve：Prepared with chromatogram methanol and obtain 500 μ g/ml dichloro quinolinic acid Standard Stock solutions, then
0.10,0.20,0.50,1.0,2.0,5.0 and 10.0 μ g/ml dichloro quinolinic acid series standard works are obtained with chromatogram methanol dilution
Make solution.Standard liquid is distinguished into sample introduction, the peak area figure related to liquor strength work of acquisition seeks coefficient correlation.Standard curve
As shown in figure 4, as a result showing that the linearly dependent coefficient r between various concentrations dichloro quinolinic acid and peak area is 0.9980；Show two
Chloro-quinolinic acid content is in good linear relationship with response.
Utilize（2）Described in testing conditions the dichloro quinolinic acid degradation capability of the bacterial strain is measured, and by following
Formula tries to achieve its dichloro quinolinic acid degradation rate：
Measurement result is as shown in figure 5, show after culture 6d, and bacterial strain J3 is 10.3% to the degradation rate of dichloro quinolinic acid；Cultivate 23d
Afterwards, bacterial strain J3 is 33.5% to the degradation rate of dichloro quinolinic acid.As can be seen here bacterial strain J3 to the dichloro quinolinic acid in soil have compared with
Good degradation capability.
The Molecular Identification of embodiment 2
Using bacterial 16 S rDNA universal primer fD2/ rP1, carried out after the 16s rDNA sequencings that bacterial strain is expanded by bacterium colony PCR
Bacterial strain is identified in comparative analysis：
fD2：5 '-AGAGTTTGATCATGGCTCAG-3 ',
rP1：5’-ACGGTTACCTTGTTACGACTT-3’；
PCR reaction systems：PCR reaction systems are：1 × PCR MIX reaction solutions（Quan Shijin bio tech ltd, Beijing）,
0.4 μM of primer, and it is used as template by the use of a small amount of bacterium colony of toothpick picking；
PCR response procedures：32 circulations are carried out after 95 DEG C of min of pre-degeneration 4, circulation every time includes 95 DEG C of 30 s of denaturation, and 58 DEG C are moved back
30 s and 72 DEG C of 1 min of extension of fire；Finally 10 min are re-extended at 72 DEG C.
PCR primer J3-16S rDNA carry out DNA recovery, sequencing after being verified through agarose gel electrophoresis, sequencing result with
Genbank databases are compared.As a result show, the Stenotrophomonas in present invention screening obtained strains J3 and Genbank belongs to
Germ oligotrophy unit cell（Stenotrophomonas maltophilia）Homology is up to 100%, and its homologous sequence accession number is
NR_112030.1.Identified, bacterial strain is Stenotrophomonas J3（Stenotrophomonas sp. J3）, it has been preserved in Chinese allusion quotation
Type culture collection CCTCC, address：Wuhan, China Wuhan University, postcode：430072；Preservation date is 2017
On March 16, in, deposit number is CCTCC NO: M 2017128.
The foregoing is only presently preferred embodiments of the present invention, all equivalent changes done according to scope of the present invention patent with
Modification, should all belong to the covering scope of the present invention.
SEQUENCE LISTING
<110>Fujian Prov. Co., China Tobacco Corp.
University Of Agriculture and Forestry In Fujian
<120>The Stenotrophomonas of one plant of pedo relict dichloro quinolinic acid that can degrade
<130> 3
<160> 3
<170> PatentIn version 3.3
<210> 1
<211> 20
<212> DNA
<213> fD2
<400> 1
agagtttgat catggctcag 20
<210> 2
<211> 21
<212> DNA
<213> rP1
<400> 2
acggttacct tgttacgact t 21
<210> 3
<211> 1411
<212> DNA
<213> J3-16S rDNA
<400> 3
gcagtcgacg gcagcacagg agagcttgct ctctgggtgg cgagtggcgg acgggtgagg 60
aatacatcgg aatctacttt ttcgtggggg ataacgtagg gaaacttacg ctaataccgc 120
atacgaccta cgggtgaaag caggggacct tcgggccttg cgcgattgaa tgagccgatg 180
tcggattagc tagttggcgg ggtaaaggcc caccaaggcg acgatccgta gctggtctga 240
gaggatgatc agccacactg gaactgagac acggtccaga ctcctacggg aggcagcagt 300
ggggaatatt ggacaatggg cgcaagcctg atccagccat accgcgtggg tgaagaaggc 360
cttcgggttg taaagccctt ttgttgggaa agaaatccag ccggctaata cctggttggg 420
atgacggtac ccaaagaata agcaccggct aacttcgtgc cagcagccgc ggtaatacga 480
agggtgcaag cgttactcgg aattactggg cgtaaagcgt gcgtaggtgg tcgtttaagt 540
ctgttgtgaa agccctgggc tcaacctggg aactgcagtg gaaactggac gactagagtg 600
tggtagaggg tagcggaatt cctggtgtag cagtgaaatg cgtagagatc aggaggaaca 660
tccatggcga aggcagctac ctggaccaac actgacactg aggcacgaaa gcgtggggag 720
caaacaggat tagataccct ggtagtccac gccctaaacg atgcgaactg gatgttgggt 780
gcaatttggc acgcagtatc gaagctaacg cgttaagttc gccgcctggg gagtacggtc 840
gcaagactga aactcaaagg aattgacggg ggcccgcaca agcggtggag tatgtggttt 900
aattcgatgc aacgcgaaga accttacctg gccttgacat gtcgagaact ttccagagat 960
ggatgggtgc cttcgggaac tcgaacacag gtgctgcatg gctgtcgtca gctcgtgtcg 1020
tgagatgttg ggttaagtcc cgcaacgagc gcaacccttg tccttagttg ccagcacgta 1080
atggtgggaa ctctaaggag accgccggtg acaaaccgga ggaaggtggg gatgacgtca 1140
agtcatcatg gcccttacgg ccagggctac acacgtacta caatggtggg gacagagggc 1200
tgcaagccgg cgacggtaag ccaatcccag aaaccccatc tcagtccgga ttggagtctg 1260
caactcgact ccatgaagtc ggaatcgcta gtaatcgcag atcagcattg ctgcggtgaa 1320
tacgttcccg ggccttgtac acaccgcccg tcacaccatg ggagtttgtt gcaccagaag 1380
caggtagctt aaccttcggg agggcgctgc c 1411
Claims (2)
1. the Stenotrophomonas of one plant of pedo relict dichloro quinolinic acid that can degrade, it is characterised in that：The bacterial strain is oligotrophy list
Born of the same parents bacterium J3（Stenotrophomonas sp. J3）, it has been preserved in China typical culture collection center CCTCC, preservation date
For on March 16th, 2017, deposit number was CCTCC No: M 2017128.
2. using one plant described in claim 1 can degrade pedo relict dichloro quinolinic acid Stenotrophomonas degraded dichloro
Application in quinolinic acid.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111378591A (en) * | 2018-12-27 | 2020-07-07 | 有研工程技术研究院有限公司 | Oligotrophomonas and method for treating benzene-containing organic waste gas by using same |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101818124A (en) * | 2010-03-15 | 2010-09-01 | 沈阳农业大学 | Stenotrophomonas capable of improving herbicide resistance in soybeans, method for preparing same and application thereof |
| CN102046778A (en) * | 2008-05-29 | 2011-05-04 | 日本烟草产业株式会社 | Bacterium capable of reducing heavy metal content in plant |
| CN102559496A (en) * | 2010-12-13 | 2012-07-11 | 北京师范大学 | Pyrene-degrading bacterial strain |
| CN102586160A (en) * | 2012-03-14 | 2012-07-18 | 中国地质大学（武汉） | Stenotrophomonas maltophilia DS4 |
-
2017
- 2017-04-22 CN CN201710267838.0A patent/CN107090415A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102046778A (en) * | 2008-05-29 | 2011-05-04 | 日本烟草产业株式会社 | Bacterium capable of reducing heavy metal content in plant |
| CN101818124A (en) * | 2010-03-15 | 2010-09-01 | 沈阳农业大学 | Stenotrophomonas capable of improving herbicide resistance in soybeans, method for preparing same and application thereof |
| CN102559496A (en) * | 2010-12-13 | 2012-07-11 | 北京师范大学 | Pyrene-degrading bacterial strain |
| CN102586160A (en) * | 2012-03-14 | 2012-07-18 | 中国地质大学（武汉） | Stenotrophomonas maltophilia DS4 |
Non-Patent Citations (5)
| Title |
|---|
| IIZUKA,T. ET AL.: ""Stenotrophomonas maltophilia strain ATCC 1 3637 1 6S ribosomal RNA gene, partial sequence"， Accession Number：NR_11 2030.1", 《GENBANK》 * |
| XIONG-ZHI ZHENG ET AL.: ""Research on the Degradation of Herbicide----Quinclorac by Microorganism"", 《ADVANCED MATERIALS RESEARCH》 * |
| ZENG WEIAI ET AL.: ""Isolation and Screening of Acetochlor-degrading Strain and Its Degradation Characteristics"", 《APPLIED MECHANICS AND MATERIALS》 * |
| ZHENMEI LU ET AL.: ""Catalase and superoxide dismutase activities in a Stenotrophomonas maltophilia WZ2 resistant to herbicide pollution"", 《ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY》 * |
| 范俊 等: ""1株二氯喹啉酸降解菌QC06的筛选鉴定及其降解特性"", 《中国生物防治学报》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111378591A (en) * | 2018-12-27 | 2020-07-07 | 有研工程技术研究院有限公司 | Oligotrophomonas and method for treating benzene-containing organic waste gas by using same |
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Application publication date: 20170825 |