CN107080544A - A kind of animal hearts blood-sampling method - Google Patents
A kind of animal hearts blood-sampling method Download PDFInfo
- Publication number
- CN107080544A CN107080544A CN201710369207.XA CN201710369207A CN107080544A CN 107080544 A CN107080544 A CN 107080544A CN 201710369207 A CN201710369207 A CN 201710369207A CN 107080544 A CN107080544 A CN 107080544A
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- China
- Prior art keywords
- animal
- blood
- entry point
- inserting needle
- sampling
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Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/15—Devices for taking samples of blood
- A61B5/153—Devices specially adapted for taking samples of venous or arterial blood, e.g. with syringes
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/15—Devices for taking samples of blood
- A61B5/150007—Details
- A61B5/150015—Source of blood
- A61B5/15003—Source of blood for venous or arterial blood
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D3/00—Appliances for supporting or fettering animals for operative purposes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B2503/00—Evaluating a particular growth phase or type of persons or animals
- A61B2503/40—Animals
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B2503/00—Evaluating a particular growth phase or type of persons or animals
- A61B2503/42—Evaluating a particular growth phase or type of persons or animals for laboratory research
Abstract
The present invention relates to a kind of animal hearts blood-sampling method, including animal is fixed, finger flicking animal chest rib triangle intersection finds xiphoid-process, pressed up again towards head position with finger, circular exoskeletal region is found, is defined as entry point, centered on entry point, the vertical inserting needle in exoskeletal area in the range of radius 1cm, blood sampling.The inventive method can realize the blood collection of the animals such as rabbit, cavy, rat, mouse, blood sampling position is easily determined, Culling heart blood method in the prior art is overcome to adopt less than blood or the not enough defect of blood sampling volume, more blood flow volume can be obtained, it is easy to operate, quick, accurate, it is small to animal hearts degree of injury, taken a blood sample beneficial to repetition.
Description
Technical field
The present invention relates to a kind of animal hearts blood-sampling method.
Background technology
The blood sampling of the animal hearts such as new zealand white rabbit is the essential link of university student's laboratory, even more numerous scientific research persons
Required skill, especially a large amount of serum prepare and need accurately to position and take a blood sample.Conventional Culling heart blood method describes scope
Wider, position is not accurate enough, and operator often fails, it is necessary to ask veteran to help through；Some multiple ability of trial
Animal hearts are damaged larger by success, are unfavorable for reusing.
The content of the invention
In view of existing deficiency, the present invention is intended to provide a kind of fast and accurately animal hearts blood-sampling method.
Technical solution of the present invention is as follows
A kind of animal hearts blood-sampling method, including：Animal is fixed, finger flicking animal chest rib triangle intersection looks for
To xiphoid-process, pressed up again towards head position with finger (preferably forefinger), find circular exoskeletal region, be defined as entry point
(heartbeat is the most obvious herein), the vertical inserting needle centered on the entry point, blood sampling.
Further, the animal includes rabbit, cavy, rat, mouse etc..Further, the animal is New Zealand
White Rabbit.
Further, the method for fixed animal includes：Animal is placed on operating desk (such as autopsy table), back down,
Upward, four limbs are fixed on making platform the outside of belly after stretching, and keep chest straight.
Further, blood sampling instrument used is syringe (such as 20mL syringes), can use No. 7 or No. 8 syringe needles.
Further, centered on entry point (center of circle), can in the range of radius 1cm vertical inserting needle.
Further, this operation is along entry point to vertical inserting needle between 1cm on the right side of animal or so place's rib, and blood is automatically flowed into
Syringe is taken a blood sample.
Further, inserting needle can not be too deep, enters needle speed slow, it is seen that have and stop inserting needle after blood back at once, it is to avoid heart
It is inserted through.
Further, when the animal is new zealand white rabbit, the depth that blood sampling syringe needl enters animal thoracic cavity is no more than
2-3cm；It can take a blood sample weekly once；Different blood volumes are taken according to the size of rabbit, can typically take a blood sample 20mL, 4kg or so every time
New zealand white rabbit can once take a blood sample 50mL every time.
Specifically, the present invention provides a kind of animal hearts blood-sampling method, and the animal is new zealand white rabbit, methods described
Including：Animal level is lain on the back and is fixed on autopsy table, keeps chest straight, finger flicking animal chest rib triangle intersection
Xiphoid-process is found, is pressed up again towards head position with forefinger, circular exoskeletal region is found, is defined as the entry point (heart herein
Dirty bounce is the most obvious), along entry point to vertical inserting needle between 1cm on the right side of animal or so place's rib, blood automatically flows into syringe
Taken a blood sample；The depth that blood sampling syringe needl enters animal thoracic cavity is no more than 2-3cm.
Beneficial effect：
The inventive method can realize the blood collection of the animals such as rabbit, cavy, rat, mouse, and blood sampling position is easily determined,
Overcome Culling heart blood method in the prior art to adopt less than blood or the not enough defect of blood sampling volume, more blood flow volume can be obtained, grasped
Make easy, quick, accurate, it is small to animal hearts degree of injury, taken a blood sample beneficial to repetition.
Brief description of the drawings
Fig. 1 represents the inventive method new zealand white rabbit Culling heart blood position.
Fig. 2 represents commonsense method new zealand white rabbit Culling heart blood position.
Fig. 3 represents the inventive method Culling heart blood inserting needle position and commonsense method Culling heart blood inserting needle position versus.
In Fig. 3：1 is commonsense method inserting needle position；2 be the inventive method inserting needle position.
Embodiment
Following examples are used to illustrate the present invention, but are not limited to the scope of the present invention.It is unreceipted specific in embodiment
Technology or condition person, are carried out according to the technology or condition described by document in the art, or according to product description.It is used
Reagent or the unreceipted production firm person of instrument, are the conventional products that can be commercially available by regular distributor.
Embodiment 1
New zealand white rabbit Culling heart blood method, concrete operation step is as follows：
1) animal level is lain on the back and be fixed on autopsy table (down, the outside of belly is upward at back), keep chest straight.
If rabbit individual is larger, autopsy table can not effectively fix rabbit, also manually actuated fixation：Two people are big by New Zealand
White rabbit Baoding is good, and a people catches two ears and forelimb, and another hind leg of people Baoding two is upward facing away from lower breast, it is impossible to allows rabbit to struggle and rocks.
The degree that animal is fixed will directly affect the effect of blood sampling.Keep new zealand white rabbit static not in blood collection procedure
It is dynamic, it is easier to take a blood sample successfully；Furthermore it is also possible to ensure that the heart of rabbit is damaged less, beneficial to the fast quick-recovery of heart, to carry out one
Blood sampling again after week.
2) the chest rib triangle intersection of left index finger flicking rabbit finds xiphoid-process, then is pressed up towards head position,
A circular exoskeletal region is found, heartbeat is the most obvious herein, is defined as entry point.
3) entry point is sterilized with 75% cotton ball soaked in alcohol, using 20mL syringes, (No. 8 syringe needles, syringe extracts few in advance
Measure air) along the entry point to vertical inserting needle 2-3cm between 1cm on the right side of animal or so place's rib, blood is automatically flowed into needle tubing.
Different blood volumes are taken according to the Individual Size difference of rabbit, can typically take a blood sample 20mL/ rabbit, 4kg or so new zealand white rabbits one
The secondary 50mL that takes a blood sample.Syringe needle is removed after blood sampling, blood is slowly injected in ready sterile centrifugation tube.
Fig. 1 shows this method new zealand white rabbit Culling heart blood position.
Comparative example 1
Common new zealand white rabbit Culling heart blood method, including fixed rabbit, make rabbit belly towards the person that takes blood.In the 3rd intercostal
Injection needle is vertically pierced into heart at left border of sternum 3mm, when syringe needle is correctly pierced into heart, and due to the strength of heartbeat, blood can nature
Into syringe；If blood back should extract syringe when bad, puncture blood collecting again after reaffirming.Slowly extract the desired amount of blood
Liquid.Action should be rapid, to shorten the time of retaining needle in heart and prevent blood clotting.Can not left and right pendulum in intrathoracic syringe needle
Move to prevent from injuring the heart, lung.
Fig. 2 shows this method new zealand white rabbit Culling heart blood position.
The inventive method Culling heart blood inserting needle position and commonsense method Culling heart blood inserting needle position versus are shown in that (animal is Fig. 3
New zealand white rabbit), wherein：1 is commonsense method inserting needle position；2 be the inventive method inserting needle position.Comparing result shows：
Generic cardiac blood-sampling method is using the 3rd intercostal as description inserting needle position, and side rib is longer, and entry point is not easy to look for
The bad inserting needle again of blood back is referred in standard, description, the uncertainty of this method is also absolutely proved, and then cause again to animal
Injury；And if the method inserting needle position is on the lower side, easily injuring lungs and causing animal dead, cause irremediable to experiment
Loss.Heartbeat is the most substantially located above present invention selection chest, in this way it is easy to determine position, then to the left or to the right in this position
The vertical inserting needles of 1cm, lock-in range is small, and blood sampling easily succeeds；In addition, this is located away from lungs, animal dead is not easily caused.
Although above the present invention is described in detail with a general description of the specific embodiments,
On the basis of the present invention, it can be made some modifications or improvements, this will be apparent to those skilled in the art.Cause
This, these modifications or improvements, belong to the scope of protection of present invention without departing from theon the basis of the spirit of the present invention.
Claims (8)
1. a kind of animal hearts blood-sampling method, it is characterised in that including：Animal is fixed, finger flicking animal chest rib three
Angle intersection finds xiphoid-process, is pressed up again towards head position with finger, finds circular exoskeletal region, is defined as inserting needle
Point, the vertical inserting needle centered on the entry point, blood sampling；The animal includes rabbit, cavy, rat, mouse.
2. according to the method described in claim 1, it is characterised in that the animal is new zealand white rabbit.
3. method according to claim 1 or 2, it is characterised in that centered on the entry point, in radius 1cm scopes
Interior vertical inserting needle.
4. method according to claim 1 or 2, it is characterised in that centered on the entry point, this operation is along described
Entry point automatically flows into syringe and taken a blood sample to vertical inserting needle between 1cm on the right side of animal or so place's rib, blood.
5. the method according to claim any one of 1-4, it is characterised in that the method for the fixed animal includes：Will be dynamic
Thing is placed on operating desk, and down, upward, four limbs are fixed on making platform the outside of belly after stretching, and keep chest straight at back.
6. the method according to claim any one of 1-4, it is characterised in that blood sampling instrument used is syringe, can use No. 7
Or No. 8 syringe needles.
7. the method according to claim any one of 1-6, it is characterised in that the animal is new zealand white rabbit, blood sampling
The depth for entering animal thoracic cavity with pin is no more than 2-3cm.
8. the method according to claim any one of 1-7, it is characterised in that the animal is new zealand white rabbit, described
Method includes：Animal level is lain on the back and is fixed on autopsy table, keeps chest straight, finger flicking animal chest rib triangle is handed over
Xiphoid-process is found at remittance, is pressed up again towards head position with forefinger, circular exoskeletal region is found, is defined as entry point, it is suitable
Entry point to vertical inserting needle between 1cm on the right side of animal or so place's rib, blood automatically flows into syringe and taken a blood sample；Blood sampling syringe needl is entered
The depth for entering animal thoracic cavity is no more than 2-3cm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710369207.XA CN107080544A (en) | 2017-05-23 | 2017-05-23 | A kind of animal hearts blood-sampling method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710369207.XA CN107080544A (en) | 2017-05-23 | 2017-05-23 | A kind of animal hearts blood-sampling method |
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| CN107080544A true CN107080544A (en) | 2017-08-22 |
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| CN201710369207.XA Pending CN107080544A (en) | 2017-05-23 | 2017-05-23 | A kind of animal hearts blood-sampling method |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108420566A (en) * | 2018-03-09 | 2018-08-21 | 重庆医药高等专科学校 | Blood-taking device for experimental mouse |
-
2017
- 2017-05-23 CN CN201710369207.XA patent/CN107080544A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108420566A (en) * | 2018-03-09 | 2018-08-21 | 重庆医药高等专科学校 | Blood-taking device for experimental mouse |
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| PB01 | Publication | ||
| PB01 | Publication | ||
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| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170822 |
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| RJ01 | Rejection of invention patent application after publication |