CN107079806A - A kind of selection of Resistant Special Rices - Google Patents

A kind of selection of Resistant Special Rices Download PDF

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Publication number
CN107079806A
CN107079806A CN201710251283.0A CN201710251283A CN107079806A CN 107079806 A CN107079806 A CN 107079806A CN 201710251283 A CN201710251283 A CN 201710251283A CN 107079806 A CN107079806 A CN 107079806A
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China
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parent
rice
resistant
strain
selection
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CN201710251283.0A
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梅淑芳
张树生
赵华
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Jinhua Polytechnic
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Jinhua Polytechnic
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Catching Or Destruction (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Pretreatment Of Seeds And Plants (AREA)

Abstract

The invention discloses a kind of selection of Resistant Special Rices, including：Disease-resistant parent I is selected, type pest-resistant parent II, Special Rices parent III is walked quickly and kept away, hybridization obtains F1(parent I/ parents II), hybridizes with parent III, obtains three and hands over F1(parent I/ parents II//parent III) seed, three hand over F1Doubled haploid (DH) colony is built using anther culture technique, DH is harvested0For seed；DH1In generation, DNA is extracted, molecular markers for identification selection carries Pi2 disease-resistant gene strains, then detects the strain of selection kind skin black；DH2Generation, interior combines outdoor great Qu and identifies that selection has the strain for walking quickly and keeping away insect resistace to planthopper and striped rice borer；DH3In generation, indica rice type is identified using Cheng's index Method, select the strain of japonica-cline type；DH4In generation, obtain the extraordinary rice varieties of black rice, the sizing strain of Resistant japonica-cline type, i.e. Resistant；The paddy rice of seed selection of the present invention, a variety of nutrition being not only rich in black rice, and because Resistant can carry out green production, suitably as the special meals rice of safe nutrition.

Description

A kind of selection of Resistant Special Rices
Technical field
The present invention relates to rice breeding technology field, especially a kind of selection of Resistant Special Rices.
Background technology
Black rice is the appreciable variety in rice, nutritious rich in iron, thick protein and fat, with good nourishing Effect, therefore be referred to as " rice of enriching blood ", " long-lived rice " etc., folks of china has saying for " meeting black must mend ".It is worth noting that, black rice General kind of the skin that retains is eaten, and residues of pesticides are relatively easily high.
China has supported the population in the world 22% with the arable land in the world 7%, at the same also consumes the world 35% chemical fertilizer and 33% agricultural chemicals.Green production and food security are increasingly becoming the focus of public attention.In addition, with Global climate change, planting Structural adjustment and cultivar change are planted, the biotic that Rice Production faces is increasingly severe.Rice blast, two move insect rice Original pest and disease damage such as plant hopper and striped rice borer frequently breaks out, and causes huge economic loss, to paddy rice caused by prevention not in time Yield and health and the serious harm of security quality are very universal.Therefore, strengthen Resistant rice variety selective, contribute to water The environment-friendly production of rice, promotes the development of safer green agriculture.
It is contemplated that polymerization black rice, 3 kinds of disease-resistant, pest-resistant good characteristics, develop safe green Rice Production, peace is cultivated The complete special meals paddy rice of nutrition.
The content of the invention
The invention provides a kind of selection of Resistant Special Rices, the sizing strain of incubation, polymerization black rice, it is disease-resistant, 3 kinds of pest-resistant good characteristics, a variety of nutrition being not only rich in black rice, and because Resistant can carry out green production, it is suitable to make For the special meals rice of safe nutrition.
The technical problems to be solved by the invention are realized by following technical scheme：
A kind of selection of Resistant Special Rices, including：
(1) selection disease resisting rice is parent I, walks quickly and keeps away type insect-proof rice for parent II, and Special Rices are parent III, carry out parent This I and parent II hybridizes, and obtains F1(parent I/ parents II) seed；
(2) F is planted1(parent I/ parents II) hybridizes for plant with parent III, obtains three and hands over F1(parent I/ parents II// Parent III) seed；
(3) F is handed in plantation three1In (parent I/ parents II//parent III) generation, doubled haploid is built using anther culture technique (DH) colony, DH is harvested by individual plant0For seed；
(4) DH is planted by strain1Generation, extract DNA, with molecular markers for identification select blast resisting strain, then observe into The kind skin of roguing system, retains the strain of kind of skin black；
(5) DH is planted by strain2Generation, interior combination outdoor great Qu identifies that selection has to planthopper and striped rice borer and walks quickly and keeps away type The elite plant strain of insect resistace；
(6) DH is planted by strain3In generation, indica rice type is identified, to the strain of japonica-cline type；
(7) DH is planted by strain4In generation, obtain black rice, disease-resistant, pest-resistant and japonica-cline type sizing strain, i.e. Resistant extraordinary Rice.
Preferably, the disease resisting rice parent I described in step (1) accounts for for China, it is the strong advantage of China Paddy Rice Inst's seed selection Indica restorer line (new varieties Authorization Notice No.：CNA004168G), the eastern seminars of China Paddy Rice Inst Zhu Xu can be purchased from.The product Blast resisting is planted, disease-resistant gene Pi2 is carried, height sense worm, to planthopper and striped rice borer without type insect resistace is walked quickly and kept away, is kind of a skin white Common rice；
It is Zhejiang University's seed selection preferably, walking quickly and keeping away type insect-proof rice parent II described in step (1) for the anti-rice in Zhejiang No. 2 Have to planthopper and striped rice borer and walk quickly and keep away type insect resistace japonica rice new varieties.The kind is handled official business to Ministry of Agriculture's Plant new variety protection Room application accepts New variety protection, application number：20130458.8, agricultural college of Zhejiang University can be purchased from.The Rice Varieties fly Lice and striped rice borer, which have, walks quickly and keeps away type insect resistace, middle sense rice blast, is kind of a common rice for skin white；
Preferably, the parental rice III described in step (1) is middle Ze Heizhen.Middle Ze Heizhen is the biological sections of Hangzhou Zhong Ze The extraordinary japonica rice variety of the black rice of skill Co., Ltd seed selection.The kind is accepted to the application of Plant new variety protection office of the Ministry of Agriculture New variety protection, application number：20150962.5, Hangzhou Zhong Ze bio tech ltd can be purchased from.The rice varieties are black rice Special Rices, height sense rice blast, height sense worm, to planthopper and striped rice borer without walking quickly and keeping away insect resistace；
Preferably, the molecular markers for identification selection disease-resistant gene described in step (5) is the Pi2 blast resistings that China accounts for carrying Gene.Specific design of primers and operating method referring to：The rice restorers such as Chen Shen China accounts for blast resisting genetic analysis and gene Identify Plant Pathologies, 2015,45 (6)：598-605；
Preferably, that being used for described in step (7) identify indica rice type is the specific methods of Cheng's index Method and behaviour Make referring to：The expensive of Jiang Yong and Li Shi are circulated a notice of the Chinese agronomy of the discriminating of 173 parts of rice material Indica or japonicas using Cheng's index Method, 2005,21 (8)：180-183,187；
Preferably, the selection China described in step (8) accounts for, the anti-rice in Zhejiang No. 2 hybridizes combo with middle Ze Heizhen and trained through flower pesticide Support, the extraordinary rice varieties of Resistant of seed selection are black rice Special Rices, plant skin black, blast resisting has to planthopper and striped rice borer Walk quickly and keep away type insect resistace.
Compared with prior art, beneficial effects of the present invention are：
(1) rice varieties that are bred as of the present invention, with black rice, blast resisting and walk quickly and keep away pest-resistant characteristic, can both utilize black A variety of nutrition of rice, can ensure edible safety with green production again；
(2) Anther Culture is used, can be judged than hybridization technique fast and stable and with Character, and uses first indica-japonica hybrid Three hybridized again with japonica rice hand over strategy, are conducive to the technical problem for overcoming anther culture of indica rice difficult.
Embodiment
The invention will be further described with reference to embodiments.The present embodiment is under premised on technical solution of the present invention Implemented, give detailed embodiment and process, but protection scope of the present invention includes but is not limited to following implementations Example.
1st, breeding material：
(1) using disease resisting rice parent I as female parent.China accounts for blast resisting, carries blast resistant gene Pi2, height sense worm, to rice Plant hopper and striped rice borer, without insect resistace is walked quickly and kept away, are kind of a common rice for skin white.
(2) with the anti-rice in Zhejiang No. 2 for male parent.The anti-rice 2 in Zhejiang has to planthopper and striped rice borer walks quickly and keeps away type insect resistace, middle sense rice blast Disease, is kind of a common rice for skin white.
(3) using middle Ze Heizhen as male parent.Middle Ze Heizhen is the extraordinary japonica rice of black rice, plants skin black, height sense rice blast, height sense Worm, to planthopper and striped rice borer without walking quickly and keeping away type insect resistace.
2nd, the Breeding Process of the extraordinary rice varieties of Resistant is：
(1) 2011 year spring and summer, Zhejiang Hangzhou is female parent with blast resisting parent I (China accounts for), with walking quickly and keeping away the pest-resistant parent of type II (the anti-rice in Zhejiang No. 2) is male parent, hybridizes to obtain F1For 8, seed；
(2) 2012 years winter-spring seasons, F is planted in Hainan Lingshui18 plants of plant, be with high zinc content parent III (middle Ze Heizhen) Paternal hybrid, obtains three and hands over F1(China accounts for the/anti-rice in Zhejiang No. 2 // middle Ze Heizhen) 91, seed；
(3) 2012 year spring and summer, Zhejiang Hangzhou plants F1(China accounts for the/anti-rice in Zhejiang No. 2 // middle Ze Heizhen) for 91 plants of plant, Doubled haploid (DH) colony is built using anther culture technique；
(4) 2013 years winter-spring seasons, Hainan Lingshui harvests DH by individual plant0For seed, 419, DH systems are built；
(5) 2013 year spring and summer, Zhejiang Hangzhou plants DH1Plant, extracts DNA, and molecular markers for identification selection carries Pi2 87 plants of disease-resistant gene, the selected strain seed of harvest, the selected strain kind skin of detection is the strain 26 of black；
(6) 2014 year spring and summer, Zhejiang Hangzhou plants DH by strain2Generation, interior combines outdoor great Qu identification identification selections There is the strain 7 for walking quickly and keeping away insect resistace to planthopper and striped rice borer；
(7) 2015 year spring and summer, Zhejiang Hangzhou plants DH by strain3In generation, indica rice class is identified using Cheng's index Method Type, selects the strain 2 of japonica-cline type；
(8) 2016 year summer and autumn, Zhejiang Hangzhou plants DH by strain4In generation, obtain black rice, the sizing of Resistant japonica-cline type Strain 1, i.e. Resistant Special Rices.
The extraordinary rice varieties of the Resistant of the present embodiment seed selection：Black rice Special Rices, plant skin black, blast resisting, to planthopper Have with striped rice borer and walk quickly and keep away type insect resistace, the good characteristic of effective polyase 13 parent.
Technical scheme and beneficial effect are described in detail embodiment described above, it should be understood that The specific embodiment of the present invention is the foregoing is only, is not intended to limit the invention, it is all to be done in the spirit of the present invention Any modification, supplement and equivalent substitution etc., should be included in the scope of the protection.

Claims (6)

1. a kind of selection of Resistant Special Rices, it is characterised in that including：
(1) selection disease resisting rice is parent I, walks quickly and keeps away type insect-proof rice for parent II, and Special Rices are parent III, carry out parent I with Parent II hybridizes, and obtains F1(parent I/ parents II) seed；
(2) F is planted1(parent I/ parents II) hybridizes for plant with parent III, obtains three and hands over F1(parent I/ parents II//parent III) seed；
(3) F is handed in plantation three1In (parent I/ parents II//parent III) generation, doubled haploid (DH) is built using anther culture technique Colony, DH is harvested by individual plant0For seed；
(4) DH is planted by strain1In generation, DNA is extracted, the strain of blast resisting is selected with molecular markers for identification, then observe selected strain Kind skin, retain the strain of kind of skin black；
(5) DH is planted by strain2Generation, interior combines outdoor great Qu and identifies that selection is pest-resistant with type is walked quickly and kept away to planthopper and striped rice borer The elite plant strain of property；
(6) DH is planted by strain3In generation, indica rice type is identified, to the strain of japonica-cline type；
(7) DH is planted by strain4In generation, obtain black rice, disease-resistant, pest-resistant and japonica-cline type sizing strain, i.e. Resistant Special Rices.
2. Resistant Special Rices selection according to claim 1, it is characterised in that walk quickly and keep away type described in step (1) Insect-proof rice parent I accounts for for China.
3. Resistant Special Rices selection according to claim 1, it is characterised in that walk quickly and keep away type described in step (1) Insect-proof rice parent II is the anti-rice in Zhejiang No. 2.
4. Resistant Special Rices selection according to claim 1, it is characterised in that the paddy rice parent described in step (1) This III is middle Ze Heizhen.
5. Resistant Special Rices selection according to claim 1, it is characterised in that the molecule mark described in step (5) Note identification selection disease-resistant gene is the Pi2 blast resistant genes that China accounts for carrying.
6. Resistant Special Rices selection according to claim 1, it is characterised in that being used for described in step (7) reflects Determine indica rice type is Cheng's index Method.
CN201710251283.0A 2017-04-14 2017-04-14 A kind of selection of Resistant Special Rices Pending CN107079806A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110896851A (en) * 2019-10-22 2020-03-24 湖南金源种业有限公司 Breeding method of early maturing rice variety

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102783407A (en) * 2012-08-15 2012-11-21 江苏丰源种业有限公司 Breeding method of high-rice-blast-resistance rice intermediate materials
CN104273023A (en) * 2014-05-15 2015-01-14 浙江大学 Polymerization breeding method for rice

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102783407A (en) * 2012-08-15 2012-11-21 江苏丰源种业有限公司 Breeding method of high-rice-blast-resistance rice intermediate materials
CN104273023A (en) * 2014-05-15 2015-01-14 浙江大学 Polymerization breeding method for rice

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110896851A (en) * 2019-10-22 2020-03-24 湖南金源种业有限公司 Breeding method of early maturing rice variety

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Application publication date: 20170822

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